Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010643

Physical Location & Seq

Genomic Coordinates Reverse (-)

11087602 .. 11093966

Gene Structure 6365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1150300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3207 bp

ATGGAAGCCAGCGCCGGACTTGTCGCAGGGTCTCACAACCGCAATGAACTCGTCGTCATTCGTGGCCATGAAGAGCACAAGCCATTGAGAAACTTGGACGGACAGGTTTGCGAAATATGCGGCGACGACGTGGGGCTGACGGTGGACGGAGACATGTTCGTGGCGTGCAACGAGTGTGGCTTCCCAGTGTGCAGGCCTTGCTACGAGTACGAGCGGAGGGAAGGCTCCCAGAACTGCCCTCAGTGCAAAACCAGATACAAGCGTCTCAAAGGCAGCCCCCGAGTCGAAGGAGACGATGACGAAGAGGACCTTGATGACATTGAACACGAATTCAACATTAATCACGATCATCCGGAAGATGATCACGATCAGATTAACGGCAACAACTCCGGCAGATCGCCCTATTATAAGCCAAACAATGAGCAGGTGGCTCAAGCCATGCTCCATGGAAAGATGAGCTATGGAAGAGGCCCTGATGATCCTGATCAGGAGTCACATTTCCCACCCGTAATCGCTGGAGTCAGATCCCGCCCGGTAAGTGGTGAGCTCACATACTCAACGCACGGTCATGGAGATCCGCAGATGTTATCATCTTCGTTGCATAAACGAGTGCATCCATATCCGGCTTCTGAGGCTGGAAGTGAAAGATGGGATGCTGAGAAGAGAGAGGGAGGTGGATGGAAAGAAAGAATGGATGACTGGAAAATGCAGCAAGGCAACCTTGGCGGCAATGATCAACCCAATGACTTTAATGATGCCGACATGGCCATGATTGATGAAGCTAGGCAGCCACTGTCAAGGAAAGTACCAATTGCGTCGAGCAAGATCAACCCTTATCGGATGATCATTGTGGCTCGGCTTTTCATTTTGGCCTTCTTCCTACGATACAGGCTTTTGCATCCGGTCAATGACGCATTTGGTCTCTGGTTAGTGTCTGTCACATGTGAAATATGGTTTGCAGTTTCATGGATCCTTGATCAGTTTCCAAAGTGGTACCCCATTGATCGTGAGACCTACCTCGATCGCCTTTCCCTCAGATATGAAAGGGAGGGTGAACCAAACATGCTCTGCCCGGTAGATGTGTTTGTGAGTACTGTGGATCCCATGAAGGAACCTCCACTTAATACCGCCAACACAGTTCTTTCAATCTTGTCCATGGACTACCCTGTTGACAAGATCTCATGCTACATCTCTGATGATGGAGCTTCCATGCTCACCTTCGAAGCCTTGTCAGAAACCGCTGAATTTGCGCGCAAATGGGTACCCTTCTGCAAGAAATTCTCCATTGAGCCTCGAGCCCCTGAGTTCTACTTCTGTGAGAAGATTGATTATCTCAAGGACAAGGTCCAGCCAACATTTGTTAAGGAGCGTCGAGCTATGAAGCGAGAGTATGAGGAATTCAAGGTTAGGATAAACGCACTTGTTGCGAAAGCCACGAAGGTTCCGCCAGAAGGGTGGATTATGCAAGATGGGACACCGTGGCCGGGAAACAACACTAAGGATCACCCTGGCATGATTCAAGTCTTTCTTGGTCACAGCGGAGGCCTTGATACCGAAGGGAATGAGCTTCCTCGTCTTGTCTACGTGTCTCGTGAGAAGAGGCCGGGTTTTCAACATCATAAGAAGGCCGGTGCCATGAATGCCCTGGTTCGTGTCTCGGGAGTACTCACAAATGCTCCTTTCATGCTCAACTTGGATTGTGATCATTATGTAAACAACAGCAAGGCTGCAAGAGAGGCCATGTGTTTCTTGATGGACCCTCAAATTGGAAGGAAGGTTTGCTATGTCCAGTTCCCTCAAAGATTTGACGGCATTGACAGAAATGATCGTTACGCGAACAGAAATACAGTCTTCTTTGATATTAACATGAAAGGTCTGGATGGAATCCAAGGTCCAGTGTATGTGGGCACAGGATGTGTGTTCAGAAGGCAAGCTTTATATGGCTACGATCCTCCGAAGGGTCCTAAGCGCCCGAAGATGGTAAGCTGTGACTGTTGCCCGTGTTTTGGACGCCGCAAGAAGCTTCCAAAGTACTCCAAGCATGATGCAAATGAAAATGCTGCAAACCTACAAGGAACGCATGATGACAAAGAGCTACTGATGTCCCAAATGAATTTTGAAAAGAAATTCGGACAGTCTGCAATTTTCGTGACTTCAACAATGATGGAACAAGGCGGTGTCCCTCCTTCCTCGAGCCCAGCAGCGATGCTTAAAGAAGCCATTCATGTGATCAGTTGCGGTTATGAAGACAAAACCGAATGGGGTTTAGAGCTAGGTTGGATTTACGGATCTATCACAGAGGACATCCTAACGGGTTTCAAGATGCATTGCCGTGGTTGGAGGTCAATTTACTGTATGCCAGATAGACCTGCATTCAAGGGTACAGCTCCCATCAACTTGTCTGATCGGCTCAACCAGGTTCTTCGTTGGGCACTTGGTTCCATTGAGATCTTTTTCAGTCGCCACAGTCCACTCTGGTATGGCTACAAGGGAGGGAAGCTCAAGTGGCTCGAGCGATTTGCTTATGTCAACACTACTGTCTACCCTTTCACCTCCCTTCCTCTTCTTGCCTATTGTATCCTCCCCGCTGTCTGCTTGCTCACTGACAAATTCATCATGCCACAGATAAGCACCTTTGCAAGTCTCTTCTTCATCGCGCTGTTTCTCTCAATCTTTGGTACTGGCATTCTTGAGCTGAGATGGAGTGGAGTGAGCATCGAAGAATGGTGGAGAAACGAGCAGTTCTGGGTGATTGGTGGTGTCTCAGCTCACCTCTTCGCCGTTGTGCAAGGTCTTCTCAAGGTTTTAGCAGGCATTGACACCAACTTCACAGTCACGGCCAAGTCATCAGATGATGAGGATTTCGGAGAATTATACGCGTTTAAATGGACAACCCTCCTCATCCCTCCAACCACAATCTTAGTCATCAACCTTGTTGGAGTCGTTGCTGGGGTTTCGGACGCCATAAACAATGGGTACCAATCATGGGGGCCTCTGTTTGGGAAGCTCTTCTTTGCTTTTTGGGTGATTGTCCATCTCTATCCCTTCCTTAAAGGTCTTATGGGGAGACAGAACCGGACGCCAACCATTGTCGTCATTTGGTCAATTCTCCTGGCCTCCATCTTCTCGTTACTCTGGGTCCGAATTGATCCATTTGTGTTGAAAACCAAGGGACCTGACACGAAACAATGCGGAATCAATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009507 GO:0009536 GO:0009664 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0010330 GO:0010393 GO:0010395 GO:0010400 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0017144 GO:0022402 GO:0030243 GO:0030244 GO:0032991 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0046527 GO:0051273 GO:0051274 GO:0051301 GO:0052546 GO:0061640 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1069

Amino Acids

121.06

Weight (kDa)

6.26

Isoelectric Point (pI)

40.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	27 - 103	2.8e-42	Zinc-binding RING-finger
Cellulose_synt	PF03552	359 - 1062	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	742 - 864	1.3e-06	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17420
fragaria_vesca	FvH4_1g00670
malus_domestica	MD02G1005600.v1.1 MD15G1150200.v1.1 MD15G1150300.v1.1
prunus_persica	Prupe.7G265400_v2.0.a1
pyrus_communis	pycom02g00450 pycom15g13420 pycom15g13430 pycom15g13440
rosa_chinensis	RchiOBHm_Chr2g0085301 RchiOBHm_Chr2g0175181
rosa_laevigata	RLG00000015676
rosa_multiflora	Rmu_sc0008510.1_g000012
rosa_rugosa	Rorug01G0459400
rosa_samantha	Rh2AG007700 Rh2BG008400 Rh2CG009100 Rh2DG009600
rosa_wichuraiana	Rw2G000740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	408
AarI	CACCTGC	1 cut(s)	415
Acc36I	ACCTGC	2 cut(s)	415, 2377
Acc65I	GGTACC	3 cut(s)	993, 1261, 2976
AccB1I	GGYRCC	4 cut(s)	993, 1261, 1631, 2976
AccBSI	CCGCTC	1 cut(s)	214
AccI	GTMKAC	2 cut(s)	1581, 2541
AccII	CGCG	4 cut(s)	1252, 1835, 2657, 2879
AccIII	TCCGGA	1 cut(s)	352
AcoI	YGGCCR	4 cut(s)	64, 765, 1481, 2838
AcsI	RAATTY	7 cut(s)	329, 1244, 1277, 1397, 2113, 2126, 2609
AcyI	GRCGYC	3 cut(s)	2011, 2961, 3080
AfiI	CCNNNNNNNGG	9 cut(s)	539, 1451, 1484, 1766, 1958, 1978, 2006, 2986, 2999
AflIII	ACRYGT	4 cut(s)	153, 941, 1584, 2877
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	4 cut(s)	1507, 1644, 2415, 3111
Alw21I	GWGCWC	2 cut(s)	78, 549
AlwNI	CAGNNNCTG	1 cut(s)	793
Ama87I	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
Aor13HI	TCCGGA	1 cut(s)	352
ApeKI	GCWGC	6 cut(s)	273, 709, 787, 1727, 2060, 2201
ApoI	RAATTY	7 cut(s)	329, 1244, 1277, 1397, 2113, 2126, 2609
AseI	ATTAAT	1 cut(s)	339
Asp700I	GAANNNNTTC	2 cut(s)	2220, 3008
Asp718I	GGTACC	3 cut(s)	993, 1261, 2976
AspLEI	GCGC	5 cut(s)	14, 1252, 1254, 1971, 2659
AspS9I	GGNCC	9 cut(s)	307, 470, 1345, 1756, 1892, 1961, 2990, 3139, 3173
AsuC2I	CCSGG	4 cut(s)	533, 1073, 1485, 1605
AsuHPI	GGTGA	8 cut(s)	554, 1064, 1207, 1496, 2542, 2761, 2762, 3037
AsuII	TTCGAA	1 cut(s)	1221
AvaI	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
AvaII	GGWCC	7 cut(s)	307, 1345, 1756, 1892, 1961, 3139, 3173
BaeGI	GKGCMC	2 cut(s)	1910, 2434
BaeI	ACNNNNGTAYC	2 cut(s)	247, 280
BalI	TGGCCA	2 cut(s)	66, 767
BamHI	GGATCC	2 cut(s)	969, 1099
BanI	GGYRCC	4 cut(s)	993, 1261, 1631, 2976
BanII	GRGCYC	3 cut(s)	549, 1300, 2198
BauI	CACGAG	1 cut(s)	1590
BbsI	GAAGAC	3 cut(s)	1843, 2253, 2786
Bbv12I	GWGCWC	2 cut(s)	78, 549
BbvI	GCAGC	6 cut(s)	285, 721, 799, 1714, 2047, 2213
BceAI	ACGGC	5 cut(s)	394, 1825, 2316, 2765, 2853
BcgI	CGANNNNNNTGC	4 cut(s)	31, 65, 2501, 2535
BciT130I	CCWGG	4 cut(s)	1509, 1646, 2417, 3113
BciVI	GTATCC	1 cut(s)	2588
BclI	TGATCA	7 cut(s)	361, 484, 733, 843, 976, 1702, 2229
BcnI	CCSGG	4 cut(s)	533, 1073, 1485, 1605
BfaI	CTAG	2 cut(s)	783, 2273
BfoI	RGCGCY	2 cut(s)	15, 1972
BfuAI	ACCTGC	2 cut(s)	415, 2377
BfuI	GTATCC	1 cut(s)	2588
BglI	GCCNNNNNGGC	1 cut(s)	764
BglII	AGATCT	2 cut(s)	1176, 2448
BisI	GCNGC	9 cut(s)	121, 274, 710, 727, 788, 1728, 2014, 2061, 2202
BlsI	GCNGC	9 cut(s)	122, 275, 711, 728, 789, 1729, 2015, 2062, 2203
BmcAI	AGTACT	3 cut(s)	1093, 1665, 2033
Bme1390I	CCNGG	8 cut(s)	533, 1073, 1485, 1509, 1605, 1646, 2417, 3113
Bme18I	GGWCC	7 cut(s)	307, 1345, 1756, 1892, 1961, 3139, 3173
BmeT110I	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	9 cut(s)	307, 470, 1345, 1756, 1892, 1961, 2990, 3139, 3173
BmrFI	CCNGG	8 cut(s)	533, 1073, 1485, 1509, 1605, 1646, 2417, 3113
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	8 cut(s)	622, 643, 745, 907, 2035, 2196, 2313, 2724
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	3 cut(s)	1843, 2253, 2786
BplI	GAGNNNNNCTC	2 cut(s)	2484, 2516
BpmI	CTGGAG	1 cut(s)	537
Bpu10I	CCTNAGC	1 cut(s)	1965
Bpu14I	TTCGAA	1 cut(s)	1221
BpuEI	CTTGAG	5 cut(s)	417, 1319, 2486, 2711, 2783
BpuMI	CCSGG	4 cut(s)	533, 1073, 1485, 1605
BsaAI	YACGTR	1 cut(s)	1585
BsaBI	GATNNNNATC	3 cut(s)	366, 483, 1701
BsaHI	GRCGYC	3 cut(s)	2011, 2961, 3080
BsaI	GGTCTC	3 cut(s)	36, 926, 1004
BsaJI	CCNNGG	9 cut(s)	445, 721, 1155, 1478, 1507, 1644, 1888, 2332, 3168
BsaWI	WCCGGW	3 cut(s)	352, 901, 3075
BsaXI	ACNNNNNCTCC	2 cut(s)	1660, 1690
Bsc4I	CCNNNNNNNGG	9 cut(s)	539, 1451, 1484, 1766, 1958, 1978, 2006, 2986, 2999
Bse118I	RCCGGY	1 cut(s)	1628
Bse1I	ACTGG	5 cut(s)	185, 704, 1789, 1895, 2686
Bse3DI	GCAATG	3 cut(s)	49, 736, 2326
Bse8I	GATNNNNATC	3 cut(s)	366, 483, 1701
BseAI	TCCGGA	1 cut(s)	352
BseBI	CCWGG	4 cut(s)	1509, 1646, 2417, 3113
BseDI	CCNNGG	9 cut(s)	445, 721, 1155, 1478, 1507, 1644, 1888, 2332, 3168
BseJI	GATNNNNATC	3 cut(s)	366, 483, 1701
BseLI	CCNNNNNNNGG	9 cut(s)	539, 1451, 1484, 1766, 1958, 1978, 2006, 2986, 2999
BseMI	GCAATG	3 cut(s)	49, 736, 2326
BseMII	CTCAG	7 cut(s)	254, 621, 648, 1048, 1293, 2687, 2778
BseNI	ACTGG	5 cut(s)	185, 704, 1789, 1895, 2686
BsePI	GCGCGC	1 cut(s)	1250
BseRI	GAGGAG	1 cut(s)	2888
BseSI	GKGCMC	2 cut(s)	1910, 2434
BseXI	GCAGC	6 cut(s)	285, 721, 799, 1714, 2047, 2213
BseYI	CCCAGC	2 cut(s)	2197, 2948
BsgI	GTGCAG	1 cut(s)	211
Bsh1236I	CGCG	4 cut(s)	1252, 1835, 2657, 2879
Bsh1285I	CGRYCG	1 cut(s)	1024
BshNI	GGYRCC	4 cut(s)	993, 1261, 1631, 2976
BsiEI	CGRYCG	1 cut(s)	1024
BsiHKAI	GWGCWC	2 cut(s)	78, 549
BsiHKCI	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
BslFI	GGGAC	4 cut(s)	1486, 2089, 2165, 3186
BslI	CCNNNNNNNGG	9 cut(s)	539, 1451, 1484, 1766, 1958, 1978, 2006, 2986, 2999
BsmBI	CGTCTC	2 cut(s)	269, 285
BsmFI	GGGAC	4 cut(s)	1486, 2089, 2165, 3186
BsmI	GAATGC	3 cut(s)	1645, 2372, 2685
Bso31I	GGTCTC	3 cut(s)	36, 926, 1004
BsoBI	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
Bsp119I	TTCGAA	1 cut(s)	1221
Bsp1286I	GDGCHC	6 cut(s)	78, 549, 1300, 1910, 2198, 2434
Bsp13I	TCCGGA	1 cut(s)	352
Bsp19I	CCATGG	2 cut(s)	445, 1155
BspCNI	CTCAG	7 cut(s)	253, 622, 649, 1047, 1294, 2688, 2777
BspEI	TCCGGA	1 cut(s)	352
BspFNI	CGCG	4 cut(s)	1252, 1835, 2657, 2879
BspMI	ACCTGC	2 cut(s)	415, 2377
BspQI	GCTCTTC	2 cut(s)	66, 3014
BspT104I	TTCGAA	1 cut(s)	1221
BspT107I	GGYRCC	4 cut(s)	993, 1261, 1631, 2976
BspTNI	GGTCTC	3 cut(s)	36, 926, 1004
BsrBI	CCGCTC	1 cut(s)	214
BsrDI	GCAATG	3 cut(s)	49, 736, 2326
BsrFI	RCCGGY	1 cut(s)	1628
BsrI	ACTGG	5 cut(s)	185, 704, 1789, 1895, 2686
BssAI	RCCGGY	1 cut(s)	1628
BssECI	CCNNGG	9 cut(s)	445, 721, 1155, 1478, 1507, 1644, 1888, 2332, 3168
BssHII	GCGCGC	1 cut(s)	1250
BssNI	GRCGYC	3 cut(s)	2011, 2961, 3080
BssSI	CACGAG	1 cut(s)	1590
BssT1I	CCWWGG	5 cut(s)	445, 721, 1155, 1888, 3168
Bst2BI	CACGAG	1 cut(s)	1590
Bst2UI	CCWGG	4 cut(s)	1509, 1646, 2417, 3113
Bst6I	CTCTTC	9 cut(s)	66, 297, 460, 656, 1592, 2568, 2651, 2780, 3014
BstACI	GRCGYC	3 cut(s)	2011, 2961, 3080
BstAPI	GCANNNNNTGC	2 cut(s)	198, 1424
BstBAI	YACGTR	1 cut(s)	1585
BstBI	TTCGAA	1 cut(s)	1221
BstC8I	GCNNGC	7 cut(s)	10, 166, 194, 1252, 1932, 2597, 2812
BstDSI	CCRYGG	4 cut(s)	445, 1155, 1478, 2332
BstFNI	CGCG	4 cut(s)	1252, 1835, 2657, 2879
BstH2I	RGCGCY	2 cut(s)	15, 1972
BstHHI	GCGC	5 cut(s)	14, 1252, 1254, 1971, 2659
BstMCI	CGRYCG	1 cut(s)	1024
BstNI	CCWGG	4 cut(s)	1509, 1646, 2417, 3113
BstNSI	RCATGY	3 cut(s)	157, 945, 1066
BstSCI	CCNGG	8 cut(s)	531, 1071, 1483, 1507, 1603, 1644, 2415, 3111
BstSLI	GKGCMC	2 cut(s)	1910, 2434
BstUI	CGCG	4 cut(s)	1252, 1835, 2657, 2879
BstV1I	GCAGC	6 cut(s)	285, 721, 799, 1714, 2047, 2213
BstV2I	GAAGAC	3 cut(s)	1843, 2253, 2786
BstX2I	RGATCY	7 cut(s)	524, 574, 969, 1099, 1176, 2288, 2448
BstXI	CCANNNNNNTGG	1 cut(s)	1455
BstYI	RGATCY	7 cut(s)	524, 574, 969, 1099, 1176, 2288, 2448
BsuI	GTATCC	1 cut(s)	2588
BtgI	CCRYGG	4 cut(s)	445, 1155, 1478, 2332
BtgZI	GCGATG	2 cut(s)	2219, 2638
BtrI	CACGTC	1 cut(s)	130
BtsIMutI	CAGTG	5 cut(s)	192, 248, 791, 1902, 2601
BveI	ACCTGC	2 cut(s)	415, 2377
Cac8I	GCNNGC	7 cut(s)	10, 166, 194, 1252, 1932, 2597, 2812
CaiI	CAGNNNCTG	1 cut(s)	793
CfoI	GCGC	5 cut(s)	14, 1252, 1254, 1971, 2659
Cfr10I	RCCGGY	1 cut(s)	1628
Cfr13I	GGNCC	9 cut(s)	307, 470, 1345, 1756, 1892, 1961, 2990, 3139, 3173
CseI	GACGC	7 cut(s)	251, 804, 920, 1358, 2019, 2969, 3088
CsiI	ACCWGGT	1 cut(s)	2415
CspCI	CAANNNNNGTGG	2 cut(s)	157, 192
DraI	TTTAAA	1 cut(s)	2884
EaeI	YGGCCR	4 cut(s)	64, 765, 1481, 2838
Eam1104I	CTCTTC	9 cut(s)	66, 297, 460, 656, 1592, 2568, 2651, 2780, 3014
EarI	CTCTTC	9 cut(s)	66, 297, 460, 656, 1592, 2568, 2651, 2780, 3014
EciI	GGCGGA	1 cut(s)	1434
Ecl136II	GAGCTC	1 cut(s)	547
Eco130I	CCWWGG	5 cut(s)	445, 721, 1155, 1888, 3168
Eco147I	AGGCCT	2 cut(s)	196, 1545
Eco24I	GRGCYC	3 cut(s)	549, 1300, 2198
Eco31I	GGTCTC	3 cut(s)	36, 926, 1004
Eco47I	GGWCC	7 cut(s)	307, 1345, 1756, 1892, 1961, 3139, 3173
Eco53kI	GAGCTC	1 cut(s)	547
Eco88I	CYCGRG	5 cut(s)	279, 1293, 1657, 2191, 2510
EcoICRI	GAGCTC	1 cut(s)	547
EcoO109I	RGGNCCY	5 cut(s)	307, 470, 1961, 2990, 3173
EcoRI	GAATTC	2 cut(s)	329, 1397
EcoRII	CCWGG	4 cut(s)	1507, 1644, 2415, 3111
EcoT14I	CCWWGG	5 cut(s)	445, 721, 1155, 1888, 3168
EcoT22I	ATGCAT	1 cut(s)	2328
EcoT38I	GRGCYC	3 cut(s)	549, 1300, 2198
ErhI	CCWWGG	5 cut(s)	445, 721, 1155, 1888, 3168
Esp3I	CGTCTC	2 cut(s)	269, 285
FaqI	GGGAC	4 cut(s)	1486, 2089, 2165, 3186
FauI	CCCGC	2 cut(s)	536, 2593
FbaI	TGATCA	7 cut(s)	361, 484, 733, 843, 976, 1702, 2229
FblI	GTMKAC	2 cut(s)	1581, 2541
Fnu4HI	GCNGC	9 cut(s)	121, 274, 710, 727, 788, 1728, 2014, 2061, 2202
FriOI	GRGCYC	3 cut(s)	549, 1300, 2198
Fsp4HI	GCNGC	9 cut(s)	121, 274, 710, 727, 788, 1728, 2014, 2061, 2202
FspBI	CTAG	2 cut(s)	783, 2273
GlaI	GCGC	5 cut(s)	13, 1251, 1253, 1970, 2658
GluI	GCNGC	9 cut(s)	121, 274, 710, 727, 788, 1728, 2014, 2061, 2202
GsaI	CCCAGC	2 cut(s)	2201, 2952
GsuI	CTGGAG	1 cut(s)	537
HaeII	RGCGCY	2 cut(s)	15, 1972
HgaI	GACGC	7 cut(s)	251, 804, 920, 1358, 2019, 2969, 3088
HhaI	GCGC	5 cut(s)	14, 1252, 1254, 1971, 2659
Hin1I	GRCGYC	3 cut(s)	2011, 2961, 3080
Hin6I	GCGC	5 cut(s)	12, 1250, 1252, 1969, 2657
HinP1I	GCGC	5 cut(s)	12, 1250, 1252, 1969, 2657
HincII	GTYRAC	2 cut(s)	1171, 2530
HindII	GTYRAC	2 cut(s)	1171, 2530
HindIII	AAGCTT	2 cut(s)	1932, 2021
HinfI	GANTC	7 cut(s)	282, 491, 519, 1516, 1884, 2940, 3195
HphI	GGTGA	8 cut(s)	554, 1064, 1207, 1496, 2542, 2761, 2762, 3037
Hpy166II	GTNNAC	8 cut(s)	145, 1055, 1171, 1582, 1714, 2471, 2530, 2542
Hpy8I	GTNNAC	8 cut(s)	145, 1055, 1171, 1582, 1714, 2471, 2530, 2542
Hpy99I	CGWCG	5 cut(s)	56, 128, 131, 820, 1374
HpyCH4IV	ACGT	2 cut(s)	129, 1584
HpySE526I	ACGT	2 cut(s)	129, 1584
Hsp92I	GRCGYC	3 cut(s)	2011, 2961, 3080
HspAI	GCGC	5 cut(s)	12, 1250, 1252, 1969, 2657
Kpn2I	TCCGGA	1 cut(s)	352
KpnI	GGTACC	3 cut(s)	997, 1265, 2980
Ksp22I	TGATCA	7 cut(s)	361, 484, 733, 843, 976, 1702, 2229
LguI	GCTCTTC	2 cut(s)	66, 3014
LmnI	GCTCC	6 cut(s)	230, 447, 1202, 1366, 1681, 2392
Lsp1109I	GCAGC	6 cut(s)	285, 721, 799, 1714, 2047, 2213
LweI	GCATC	8 cut(s)	622, 643, 745, 907, 2035, 2196, 2313, 2724
MabI	ACCWGGT	1 cut(s)	2415
MaeI	CTAG	2 cut(s)	783, 2273
MaeII	ACGT	2 cut(s)	129, 1584
MaeIII	GTNAC	8 cut(s)	492, 937, 1532, 1829, 1988, 2149, 2833, 3129
MbiI	CCGCTC	1 cut(s)	214
MfeI	CAATTG	2 cut(s)	810, 3199
MflI	RGATCY	7 cut(s)	524, 574, 969, 1099, 1176, 2288, 2448
MhlI	GDGCHC	6 cut(s)	78, 549, 1300, 1910, 2198, 2434
MlsI	TGGCCA	2 cut(s)	66, 767
MluI	ACGCGT	1 cut(s)	2877
MluNI	TGGCCA	2 cut(s)	66, 767
MlyI	GAGTC	4 cut(s)	291, 500, 528, 2949
MmeI	TCCRAC	4 cut(s)	2258, 2318, 2917, 2933
Mox20I	TGGCCA	2 cut(s)	66, 767
Mph1103I	ATGCAT	1 cut(s)	2328
MroI	TCCGGA	1 cut(s)	352
MroXI	GAANNNNTTC	2 cut(s)	2220, 3008
MscI	TGGCCA	2 cut(s)	66, 767
MseI	TTAA	9 cut(s)	339, 375, 750, 1122, 1362, 1863, 2211, 2883, 3051
MslI	CAYNNNNRTG	5 cut(s)	158, 567, 767, 2225, 2331
Msp20I	TGGCCA	2 cut(s)	66, 767
MspA1I	CMGCKG	3 cut(s)	1241, 1539, 2588
MspR9I	CCNGG	8 cut(s)	533, 1073, 1485, 1509, 1605, 1646, 2417, 3113
MunI	CAATTG	2 cut(s)	810, 3199
Mva1269I	GAATGC	3 cut(s)	1645, 2372, 2685
MvaI	CCWGG	4 cut(s)	1509, 1646, 2417, 3113
MvnI	CGCG	4 cut(s)	1252, 1835, 2657, 2879
NciI	CCSGG	4 cut(s)	533, 1073, 1485, 1605
NcoI	CCATGG	2 cut(s)	445, 1155
NmeAIII	GCCGAG	1 cut(s)	835
NmuCI	GTSAC	6 cut(s)	492, 937, 1532, 1988, 2149, 2833
NsiI	ATGCAT	1 cut(s)	2328
NspI	RCATGY	3 cut(s)	157, 945, 1066
NspV	TTCGAA	1 cut(s)	1221
PaeR7I	CTCGAG	3 cut(s)	1293, 2191, 2510
PaqCI	CACCTGC	1 cut(s)	415
PauI	GCGCGC	1 cut(s)	1250
PceI	AGGCCT	2 cut(s)	196, 1545
PciI	ACATGT	2 cut(s)	153, 941
PciSI	GCTCTTC	2 cut(s)	66, 3014
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PctI	GAATGC	3 cut(s)	1645, 2372, 2685
PdmI	GAANNNNTTC	2 cut(s)	2220, 3008
PfeI	GAWTC	3 cut(s)	1516, 1884, 3195
PflFI	GACNNNGTC	1 cut(s)	1343
PkrI	GCNGC	9 cut(s)	122, 275, 711, 728, 789, 1729, 2015, 2062, 2203
Ple19I	CGATCG	1 cut(s)	1024
PleI	GAGTC	4 cut(s)	290, 499, 527, 2948
PpsI	GAGTC	4 cut(s)	290, 499, 527, 2948
Ppu21I	YACGTR	1 cut(s)	1585
PpuMI	RGGWCCY	3 cut(s)	307, 1961, 3173
PscI	ACATGT	2 cut(s)	153, 941
PshBI	ATTAAT	1 cut(s)	339
PsiI	TTATAA	1 cut(s)	408
Psp124BI	GAGCTC	1 cut(s)	549
Psp5II	RGGWCCY	3 cut(s)	307, 1961, 3173
Psp6I	CCWGG	4 cut(s)	1507, 1644, 2415, 3111
PspFI	CCCAGC	2 cut(s)	2197, 2948
PspGI	CCWGG	4 cut(s)	1507, 1644, 2415, 3111
PspPI	GGNCC	9 cut(s)	307, 470, 1345, 1756, 1892, 1961, 2990, 3139, 3173
PspPPI	RGGWCCY	3 cut(s)	307, 1961, 3173
PspXI	VCTCGAGB	3 cut(s)	1293, 2191, 2510
PstNI	CAGNNNCTG	1 cut(s)	793
PsuI	RGATCY	7 cut(s)	524, 574, 969, 1099, 1176, 2288, 2448
PsyI	GACNNNGTC	1 cut(s)	1343
PteI	GCGCGC	1 cut(s)	1250
PvuI	CGATCG	1 cut(s)	1024
RseI	CAYNNNNRTG	5 cut(s)	158, 567, 767, 2225, 2331
SacI	GAGCTC	1 cut(s)	549
SapI	GCTCTTC	2 cut(s)	66, 3014
SaqAI	TTAA	9 cut(s)	339, 375, 750, 1122, 1362, 1863, 2211, 2883, 3051
SatI	GCNGC	9 cut(s)	121, 274, 710, 727, 788, 1728, 2014, 2061, 2202
Sau96I	GGNCC	9 cut(s)	307, 470, 1345, 1756, 1892, 1961, 2990, 3139, 3173
ScaI	AGTACT	3 cut(s)	1093, 1665, 2033
SchI	GAGTC	4 cut(s)	291, 500, 528, 2949
ScrFI	CCNGG	8 cut(s)	533, 1073, 1485, 1509, 1605, 1646, 2417, 3113
SduI	GDGCHC	6 cut(s)	78, 549, 1300, 1910, 2198, 2434
SexAI	ACCWGGT	1 cut(s)	2415
SfaNI	GCATC	8 cut(s)	622, 643, 745, 907, 2035, 2196, 2313, 2724
Sfr274I	CTCGAG	3 cut(s)	1293, 2191, 2510
SfuI	TTCGAA	1 cut(s)	1221
SinI	GGWCC	7 cut(s)	307, 1345, 1756, 1892, 1961, 3139, 3173
SlaI	CTCGAG	3 cut(s)	1293, 2191, 2510
SmiMI	CAYNNNNRTG	5 cut(s)	158, 567, 767, 2225, 2331
SmlI	CTYRAG	8 cut(s)	432, 1293, 1334, 2191, 2501, 2510, 2690, 2798
SmoI	CTYRAG	8 cut(s)	432, 1293, 1334, 2191, 2501, 2510, 2690, 2798
SseBI	AGGCCT	2 cut(s)	196, 1545
SspMI	CTAG	2 cut(s)	783, 2273
SstI	GAGCTC	1 cut(s)	549
StuI	AGGCCT	2 cut(s)	196, 1545
StyD4I	CCNGG	8 cut(s)	531, 1071, 1483, 1507, 1603, 1644, 2415, 3111
StyI	CCWWGG	5 cut(s)	445, 721, 1155, 1888, 3168
TaiI	ACGT	2 cut(s)	132, 1587
TaqI	TCGA	9 cut(s)	285, 818, 1020, 1221, 1294, 1372, 2192, 2511, 2718
TatI	WGTACW	3 cut(s)	1091, 1663, 2031
TauI	GCSGC	3 cut(s)	123, 729, 2016
TfiI	GAWTC	3 cut(s)	1516, 1884, 3195
Tru1I	TTAA	9 cut(s)	339, 375, 750, 1122, 1362, 1863, 2211, 2883, 3051
Tru9I	TTAA	9 cut(s)	339, 375, 750, 1122, 1362, 1863, 2211, 2883, 3051
TscAI	CASTG	5 cut(s)	192, 248, 798, 1902, 2608
TseFI	GTSAC	6 cut(s)	492, 937, 1532, 1988, 2149, 2833
TseI	GCWGC	6 cut(s)	273, 709, 787, 1727, 2060, 2201
Tsp45I	GTSAC	6 cut(s)	492, 937, 1532, 1988, 2149, 2833
TspGWI	ACGGA	3 cut(s)	114, 162, 2301
TspRI	CASTG	5 cut(s)	192, 248, 798, 1902, 2608
Tth111I	GACNNNGTC	1 cut(s)	1343
VpaK11BI	GGWCC	7 cut(s)	307, 1345, 1756, 1892, 1961, 3139, 3173
VspI	ATTAAT	1 cut(s)	339
XapI	RAATTY	7 cut(s)	329, 1244, 1277, 1397, 2113, 2126, 2609
XceI	RCATGY	3 cut(s)	157, 945, 1066
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1642
XhoI	CTCGAG	3 cut(s)	1293, 2191, 2510
XmiI	GTMKAC	2 cut(s)	1581, 2541
XmnI	GAANNNNTTC	2 cut(s)	2220, 3008
XspI	CTAG	2 cut(s)	783, 2273
ZrmI	AGTACT	3 cut(s)	1093, 1665, 2033
Zsp2I	ATGCAT	1 cut(s)	2328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.