Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010643

Physical Location & Seq

Genomic Coordinates Reverse (-)

11078068 .. 11084582

Gene Structure 6515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1150200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3039 bp

ATGGAAGCCAGCGCCGGACTTGTTGCAGGGTCTCACAACCGCAATGAACTCGTCGTCATTCATGGCCATGAAGAGCACAAGCCATCGAGGAACTTGGACGTACAGGTGTGCGAGATATGCGGCGATGGCGTGGGGTTGACGGTGGACGGAAACTTGTTCGTGGCGTGCAACGATTGTGGCTTCCCAGTGTGCAGGCCTTGCTACGAGTACGAGCGGAGGGAAGGCTCCCAGAACTGCCCTCAGTGCAAAACCAGATACAAGCGTCTCAAAGGCAGCCCCCGAATCGAAGGAGACGACGACGAAGAGGACATTGATGACATTGAACACGAATTCAACATTAATCACGATCATCCGGAAGATGATCACGATCAGATTAACGGCAACAACGCCGCCAGATCGCCCTATTATAAGACTAACAATAAGCAGCTGGCTGAAGCCCAGCTCCGTGGAACGAAGAGCTATGGAAGAGGCCCTGATGATCCTGATCGGGAGTCACATATCCCACCCGTAATCGCCGGAGTCAGATCCCACACGGTAATGCAGCAGGGCAACCTTGGCGGCAGTGATCAACCTGACGGATTGAATGATGCCGACATGGCCATGATAGATGGAGCTAGGCAGCCACTCTCAAGGAAAGTACCAATTGCGTTGGGCAAGATCAACCCTTATCGGATGATCATTGTGGCTCGGCTTTTCATTTTAGCATTGTTTCTTCGATACAGGCTTTTGAATCCGGTCAATGACGCATTTGGTCTCTGGTTAGTGTCTGTCATGTGTGAAATATGGTTTGCAGTTTCATGGATCCTTGATCAGTTCCCAAAGTGGTACCCCATTGATCGTAAGACCTACCTCGATCGCCTTTCCCTCAGATATGAGAGGGAGGGTGAACCGAACATGCTCTGCTCGGTAGATGTGTTTGTGAGTACTGTGGATCCCATGAAAGAACCTCCACTTAATACCGCCAACACTGTTCTTTCAATCTTGTCCATGGACTACCCTGTCGACAAGATCTCATGCTACATCTCTGATGATGGAGCTTCCATGCTCACCTTCGAAGCCTTGTCAGAAACCGCTGAATTTGCACGCAAATGGGTACCTTTCAGCAAGAAATTCTCTATTGAGCCTCGAGCCCCTGAGTTCTACTTCTGTGAGAAGATTGATTATCTCAAGGACAAGGCCCAGCCAACATTTGTTGAGGAGCGTCGAGCTATGAAGAGAGAGTACGAGGAATTCAAGGTTAGGATAAACGCACTTGTTGCGAAATCCACAAAGGTTCCGCCAGAAGGGTGGATTATGCAGGATGGGACACCGTGGCCGGGAAACAACACTCAGGATCACCCTGGCATGATCCAAGTCTTTCTTGGTCACAGCGGAGGCCTTGATACCGAAGGGAATGAGCTTCCTCGTCTTGTCTACATGTCTCGTGAGAAGAGGCCTGGTTTTCAACATCATAAGAAGGCCGGTGCCATGAATGCCCTGGTTCGTGTCTCGGGAGTACTTACAAATGCTCCTTTCCTGCTCAACTTGGATTGTGATCATTATGTAAACAACAGCAAGGCTGCAAGAGAGGCCATGTGTTTCTTGATGGACCCTCAAATTGGAAGGAAGGTTTGTTATGTCCAGTTCCCTCAAAGATTTGACGGCATTGACCGAAATGATCGTTACGCGAACAGAAATAGAATCTTTTTCGATATTAACATGAAAGGTCTGGATGGAATTCAAGGTCCTGTGTGTGTGGGCACAGGATGTGTGTTCAGAAGGCAAGCTTTATATGGCTATGATCCTCCGAAGGGTCCTAAGCGCCCAAAGATGGCAAGCTGTGACTGCTTCCCGTGTATTGGACGCCGCAAGAAGCTTCCAAAGTACTCCAAGGATGATGCAATTGGAGATGCTCCAAACCTACAAGGAATGCATGGTGACAAAAAGCTACTGATGTCCCAAATGAATTTTGAAAAGAAATTCGGACAGTCTGCAATTTTCGTGACTTCAACAATGATGGAACAAGGCGGTGTCCCTTCTTCCTCAAGCCCAGCAGCATTGCTTAAAGAAGCCATTCTTGTGATCAGTTGCGGTTATGAAGACAAAACTGATTGGGGTTTAGAGCTAGGTTGGATTTACGGATCTGTCACAGAGGACATCTTAACGGGTTTCAAGATGCATTGCCGTGGTTGGAGGTCAATTTACTGTATGCCAGATAGACCTGCATTCAAGGGTACAGCTCCCATCAACTTGTCAGATCGGCTCAACCAGGTTCTTCGTTGGGCACTTGGTTCCATTGAGATCTTTTTCAGTCGCCACAGTCCACTCTGGTATGGCTACAAGGGAGGGAAGCTCAAGTGGCTCGAGCGATTTGCTTATGTCAACACTACTGTCTACCCTTTCACCTCCCTTCCTCTTCTTGCCTATTGTATCCTCCCCGCCGTCTGCTTGCTCACTGATAAATTCATCATGCCAGAGATAAGCACTTTTGCAAGCCTCATCGTCATTGCGCTGTTTCTCTCAATCTTTGGAACTGGCATTCTTGAGCTTAGATGGAGCGGAGTGAGCATCGAAGAATGGTGGAGAAACGAGCAATTCTGGGTGATTGGTGGTGTCTCAGCTTACCTCTTCGCTGTTGTGCAAGGTCTTCTCAAGGTTTTAGGCGGCAATGACACCAATTTCACAGTCACAGCCAAGTCACCAGATGATGAGGATTTTGGAGAATTATACGCGTTGAAATGGACAACCCTCCTCATCCCTCCAACCACAATCTTAGTCATCAACCTTATTGGAGTCGTTGCTGGGATTTCGGACGCCGTAAACAACGGGAACCAATCTATGGGGCCTCTTCTTGGGAAGCTCTTCTTTGCTTTTTGGGTGATCGTTCATCTCTATCCCTTCCTTAAAGGTCTTCTGGGGAGGCAGAACCGGACGCCAACCATTGTCATCATTTGGTCAATTCTCCTGGCTTCCATCTTCTCGTTACTCTGGATCCGAATTGATCCATTTGTGTTGCAAACCAAGGGACCTGACACGAAACAGTGCGGAATCAATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009507 GO:0009536 GO:0009664 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0010330 GO:0010393 GO:0010395 GO:0010400 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0017144 GO:0022402 GO:0030243 GO:0030244 GO:0032991 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0046527 GO:0051273 GO:0051274 GO:0051301 GO:0052546 GO:0061640 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1013

Amino Acids

113.97

Weight (kDa)

6.58

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	27 - 103	2.9e-40	Zinc-binding RING-finger
Cellulose_synt	PF03552	303 - 1006	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	670 - 808	4.2e-07	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17420
fragaria_vesca	FvH4_1g00670
malus_domestica	MD02G1005600.v1.1 MD15G1150200.v1.1 MD15G1150300.v1.1
prunus_persica	Prupe.7G265400_v2.0.a1
pyrus_communis	pycom02g00450 pycom15g13420 pycom15g13430 pycom15g13440
rosa_chinensis	RchiOBHm_Chr2g0085301 RchiOBHm_Chr2g0175181
rosa_laevigata	RLG00000015676
rosa_multiflora	Rmu_sc0008510.1_g000012
rosa_rugosa	Rorug01G0459400
rosa_samantha	Rh2AG007700 Rh2BG008400 Rh2CG009100 Rh2DG009600
rosa_wichuraiana	Rw2G000740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	408
AasI	GACNNNNNNGTC	1 cut(s)	998
Acc36I	ACCTGC	1 cut(s)	2209
Acc65I	GGTACC	2 cut(s)	825, 1093
AccB1I	GGYRCC	3 cut(s)	825, 1093, 1463
AccB7I	CCANNNNNTGG	1 cut(s)	2818
AccBSI	CCGCTC	2 cut(s)	214, 2538
AccI	GTMKAC	3 cut(s)	1002, 1413, 2373
AccII	CGCG	2 cut(s)	1667, 2711
AccIII	TCCGGA	1 cut(s)	352
AcoI	YGGCCR	3 cut(s)	64, 597, 1313
AcsI	RAATTY	8 cut(s)	329, 1076, 1109, 1229, 1716, 1945, 1958, 2441
AcuI	CTGAAG	1 cut(s)	453
AcyI	GRCGYC	3 cut(s)	1843, 2793, 2912
AfiI	CCNNNNNNNGG	8 cut(s)	1283, 1316, 1598, 1790, 1810, 1838, 2818, 2831
AflIII	ACRYGT	2 cut(s)	1416, 2709
AjnI	CCWGG	5 cut(s)	1339, 1435, 1476, 2247, 2943
Alw21I	GWGCWC	1 cut(s)	78
Alw26I	GTCTC	7 cut(s)	36, 269, 285, 758, 1425, 1492, 2599
Ama87I	CYCGRG	3 cut(s)	1125, 1489, 2342
Aor13HI	TCCGGA	1 cut(s)	352
ApeKI	GCWGC	6 cut(s)	273, 424, 541, 619, 1559, 2033
ApoI	RAATTY	8 cut(s)	329, 1076, 1109, 1229, 1716, 1945, 1958, 2441
AseI	ATTAAT	1 cut(s)	339
Asp700I	GAANNNNTTC	2 cut(s)	2052, 2840
Asp718I	GGTACC	2 cut(s)	825, 1093
AspLEI	GCGC	3 cut(s)	14, 1803, 2491
AspS9I	GGNCC	7 cut(s)	470, 1177, 1588, 1724, 1793, 2822, 3005
AsuC2I	CCSGG	1 cut(s)	1317
AsuHPI	GGTGA	8 cut(s)	896, 1039, 1328, 1928, 2374, 2593, 2670, 2869
AsuII	TTCGAA	1 cut(s)	1053
AvaI	CYCGRG	3 cut(s)	1125, 1489, 2342
AvaII	GGWCC	4 cut(s)	1588, 1724, 1793, 3005
BaeGI	GKGCMC	2 cut(s)	1742, 2266
BaeI	ACNNNNGTAYC	2 cut(s)	247, 280
BalI	TGGCCA	2 cut(s)	66, 599
BamHI	GGATCC	3 cut(s)	801, 931, 2970
BanI	GGYRCC	3 cut(s)	825, 1093, 1463
BanII	GRGCYC	1 cut(s)	1132
BarI	GAAGNNNNNNTAC	2 cut(s)	1205, 1237
BauI	CACGAG	1 cut(s)	1422
BbsI	GAAGAC	3 cut(s)	2085, 2618, 2882
Bbv12I	GWGCWC	1 cut(s)	78
BbvI	GCAGC	6 cut(s)	285, 436, 553, 631, 1546, 2045
BceAI	ACGGC	5 cut(s)	394, 1657, 2148, 2405, 2780
BcgI	CGANNNNNNTGC	4 cut(s)	31, 65, 2333, 2367
BciT130I	CCWGG	5 cut(s)	1341, 1437, 1478, 2249, 2945
BciVI	GTATCC	1 cut(s)	2420
BclI	TGATCA	6 cut(s)	361, 565, 675, 808, 1534, 2061
BcnI	CCSGG	1 cut(s)	1317
BcoDI	GTCTC	7 cut(s)	36, 269, 285, 758, 1425, 1492, 2599
BfaI	CTAG	2 cut(s)	615, 2105
BfoI	RGCGCY	2 cut(s)	15, 1804
BfuAI	ACCTGC	1 cut(s)	2209
BfuI	GTATCC	1 cut(s)	2420
BglI	GCCNNNNNGGC	1 cut(s)	596
BglII	AGATCT	2 cut(s)	1008, 2280
BmcAI	AGTACT	3 cut(s)	925, 1497, 1865
Bme1390I	CCNGG	6 cut(s)	1317, 1341, 1437, 1478, 2249, 2945
Bme18I	GGWCC	4 cut(s)	1588, 1724, 1793, 3005
BmeT110I	CYCGRG	3 cut(s)	1125, 1489, 2342
BmgT120I	GGNCC	7 cut(s)	470, 1177, 1588, 1724, 1793, 2822, 3005
BmrFI	CCNGG	6 cut(s)	1317, 1341, 1437, 1478, 2249, 2945
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	5 cut(s)	577, 1867, 1879, 2145, 2556
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	3 cut(s)	2085, 2618, 2882
BplI	GAGNNNNNCTC	2 cut(s)	2316, 2348
Bpu10I	CCTNAGC	1 cut(s)	1797
Bpu14I	TTCGAA	1 cut(s)	1053
BpuEI	CTTGAG	6 cut(s)	613, 1151, 2008, 2318, 2543, 2615
BpuMI	CCSGG	1 cut(s)	1317
BsaBI	GATNNNNATC	3 cut(s)	366, 483, 1533
BsaHI	GRCGYC	3 cut(s)	1843, 2793, 2912
BsaI	GGTCTC	2 cut(s)	36, 758
BsaJI	CCNNGG	9 cut(s)	445, 553, 987, 1310, 1339, 1476, 1869, 2164, 3000
BsaWI	WCCGGW	3 cut(s)	352, 733, 2907
BsaXI	ACNNNNNCTCC	2 cut(s)	1492, 1522
Bsc4I	CCNNNNNNNGG	8 cut(s)	1283, 1316, 1598, 1790, 1810, 1838, 2818, 2831
Bse118I	RCCGGY	1 cut(s)	1460
Bse1I	ACTGG	3 cut(s)	185, 1621, 2518
Bse3DI	GCAATG	5 cut(s)	49, 2036, 2158, 2484, 2653
Bse8I	GATNNNNATC	3 cut(s)	366, 483, 1533
BseAI	TCCGGA	1 cut(s)	352
BseBI	CCWGG	5 cut(s)	1341, 1437, 1478, 2249, 2945
BseDI	CCNNGG	9 cut(s)	445, 553, 987, 1310, 1339, 1476, 1869, 2164, 3000
BseGI	GGATG	7 cut(s)	349, 678, 1306, 1717, 1751, 1879, 2733
BseJI	GATNNNNATC	3 cut(s)	366, 483, 1533
BseLI	CCNNNNNNNGG	8 cut(s)	1283, 1316, 1598, 1790, 1810, 1838, 2818, 2831
BseMI	GCAATG	5 cut(s)	49, 2036, 2158, 2484, 2653
BseMII	CTCAG	5 cut(s)	254, 880, 1125, 1343, 2610
BseNI	ACTGG	3 cut(s)	185, 1621, 2518
BseRI	GAGGAG	2 cut(s)	1211, 2720
BseSI	GKGCMC	2 cut(s)	1742, 2266
BseXI	GCAGC	6 cut(s)	285, 436, 553, 631, 1546, 2045
BseYI	CCCAGC	4 cut(s)	438, 1179, 2029, 2780
BsgI	GTGCAG	1 cut(s)	211
Bsh1236I	CGCG	2 cut(s)	1667, 2711
Bsh1285I	CGRYCG	1 cut(s)	856
BshNI	GGYRCC	3 cut(s)	825, 1093, 1463
BsiEI	CGRYCG	1 cut(s)	856
BsiHKAI	GWGCWC	1 cut(s)	78
BsiHKCI	CYCGRG	3 cut(s)	1125, 1489, 2342
BsiSI	CCGG	7 cut(s)	15, 353, 516, 734, 1316, 1461, 2908
BslFI	GGGAC	4 cut(s)	1318, 1921, 1997, 3018
BslI	CCNNNNNNNGG	8 cut(s)	1283, 1316, 1598, 1790, 1810, 1838, 2818, 2831
BsmAI	GTCTC	7 cut(s)	36, 269, 285, 758, 1425, 1492, 2599
BsmBI	CGTCTC	2 cut(s)	269, 285
BsmFI	GGGAC	4 cut(s)	1318, 1921, 1997, 3018
BsmI	GAATGC	4 cut(s)	1477, 1914, 2204, 2517
Bso31I	GGTCTC	2 cut(s)	36, 758
BsoBI	CYCGRG	3 cut(s)	1125, 1489, 2342
Bsp119I	TTCGAA	1 cut(s)	1053
Bsp1286I	GDGCHC	4 cut(s)	78, 1132, 1742, 2266
Bsp13I	TCCGGA	1 cut(s)	352
Bsp19I	CCATGG	1 cut(s)	987
BspCNI	CTCAG	5 cut(s)	253, 879, 1126, 1342, 2609
BspEI	TCCGGA	1 cut(s)	352
BspFNI	CGCG	2 cut(s)	1667, 2711
BspMI	ACCTGC	1 cut(s)	2209
BspQI	GCTCTTC	3 cut(s)	66, 449, 2846
BspT104I	TTCGAA	1 cut(s)	1053
BspT107I	GGYRCC	3 cut(s)	825, 1093, 1463
BspTNI	GGTCTC	2 cut(s)	36, 758
BsrBI	CCGCTC	2 cut(s)	214, 2538
BsrDI	GCAATG	5 cut(s)	49, 2036, 2158, 2484, 2653
BsrFI	RCCGGY	1 cut(s)	1460
BsrI	ACTGG	3 cut(s)	185, 1621, 2518
BssAI	RCCGGY	1 cut(s)	1460
BssECI	CCNNGG	9 cut(s)	445, 553, 987, 1310, 1339, 1476, 1869, 2164, 3000
BssNI	GRCGYC	3 cut(s)	1843, 2793, 2912
BssSI	CACGAG	1 cut(s)	1422
BssT1I	CCWWGG	4 cut(s)	553, 987, 1869, 3000
Bst2BI	CACGAG	1 cut(s)	1422
Bst2UI	CCWGG	5 cut(s)	1341, 1437, 1478, 2249, 2945
BstACI	GRCGYC	3 cut(s)	1843, 2793, 2912
BstAPI	GCANNNNNTGC	2 cut(s)	198, 1256
BstBI	TTCGAA	1 cut(s)	1053
BstC8I	GCNNGC	9 cut(s)	10, 166, 194, 429, 1084, 1764, 1816, 2429, 2473
BstDEI	CTNAG	8 cut(s)	240, 866, 1134, 1329, 1797, 2528, 2596, 2752
BstDSI	CCRYGG	4 cut(s)	445, 987, 1310, 2164
BstF5I	GGATG	7 cut(s)	349, 678, 1306, 1717, 1751, 1879, 2733
BstFNI	CGCG	2 cut(s)	1667, 2711
BstH2I	RGCGCY	2 cut(s)	15, 1804
BstHHI	GCGC	3 cut(s)	14, 1803, 2491
BstMAI	GTCTC	7 cut(s)	36, 269, 285, 758, 1425, 1492, 2599
BstMCI	CGRYCG	1 cut(s)	856
BstNI	CCWGG	5 cut(s)	1341, 1437, 1478, 2249, 2945
BstNSI	RCATGY	2 cut(s)	898, 1420
BstSCI	CCNGG	6 cut(s)	1315, 1339, 1435, 1476, 2247, 2943
BstSLI	GKGCMC	2 cut(s)	1742, 2266
BstUI	CGCG	2 cut(s)	1667, 2711
BstV1I	GCAGC	6 cut(s)	285, 436, 553, 631, 1546, 2045
BstV2I	GAAGAC	3 cut(s)	2085, 2618, 2882
BstX2I	RGATCY	7 cut(s)	524, 801, 931, 1008, 2120, 2280, 2970
BstXI	CCANNNNNNTGG	2 cut(s)	446, 1287
BstYI	RGATCY	7 cut(s)	524, 801, 931, 1008, 2120, 2280, 2970
BsuI	GTATCC	1 cut(s)	2420
BtgI	CCRYGG	4 cut(s)	445, 987, 1310, 2164
BtgZI	GCGATG	1 cut(s)	138
BtsCI	GGATG	7 cut(s)	349, 678, 1306, 1717, 1751, 1879, 2733
BtsI	GCAGTG	1 cut(s)	568
BtsIMutI	CAGTG	6 cut(s)	192, 248, 568, 966, 2433, 3026
BveI	ACCTGC	1 cut(s)	2209
Cac8I	GCNNGC	9 cut(s)	10, 166, 194, 429, 1084, 1764, 1816, 2429, 2473
CfoI	GCGC	3 cut(s)	14, 1803, 2491
Cfr10I	RCCGGY	1 cut(s)	1460
Cfr13I	GGNCC	7 cut(s)	470, 1177, 1588, 1724, 1793, 2822, 3005
CseI	GACGC	6 cut(s)	251, 752, 1190, 1851, 2801, 2920
CsiI	ACCWGGT	1 cut(s)	2247
CspCI	CAANNNNNGTGG	2 cut(s)	157, 192
DdeI	CTNAG	8 cut(s)	240, 866, 1134, 1329, 1797, 2528, 2596, 2752
DrdI	GACNNNNNNGTC	1 cut(s)	998
DseDI	GACNNNNNNGTC	1 cut(s)	998
EaeI	YGGCCR	3 cut(s)	64, 597, 1313
EciI	GGCGGA	1 cut(s)	1266
Eco130I	CCWWGG	4 cut(s)	553, 987, 1869, 3000
Eco147I	AGGCCT	3 cut(s)	196, 1377, 1435
Eco24I	GRGCYC	1 cut(s)	1132
Eco31I	GGTCTC	2 cut(s)	36, 758
Eco47I	GGWCC	4 cut(s)	1588, 1724, 1793, 3005
Eco57I	CTGAAG	1 cut(s)	453
Eco88I	CYCGRG	3 cut(s)	1125, 1489, 2342
EcoO109I	RGGNCCY	5 cut(s)	470, 1724, 1793, 2822, 3005
EcoRI	GAATTC	3 cut(s)	329, 1229, 1716
EcoRII	CCWGG	5 cut(s)	1339, 1435, 1476, 2247, 2943
EcoT14I	CCWWGG	4 cut(s)	553, 987, 1869, 3000
EcoT22I	ATGCAT	2 cut(s)	1914, 2160
EcoT38I	GRGCYC	1 cut(s)	1132
ErhI	CCWWGG	4 cut(s)	553, 987, 1869, 3000
Esp3I	CGTCTC	2 cut(s)	269, 285
FalI	AAGNNNNNCTT	8 cut(s)	1344, 1376, 1750, 1782, 2040, 2072, 2828, 2860
FaqI	GGGAC	4 cut(s)	1318, 1921, 1997, 3018
FauI	CCCGC	1 cut(s)	2425
FbaI	TGATCA	6 cut(s)	361, 565, 675, 808, 1534, 2061
FblI	GTMKAC	3 cut(s)	1002, 1413, 2373
FokI	GGATG	7 cut(s)	336, 685, 1313, 1724, 1758, 1886, 2720
FriOI	GRGCYC	1 cut(s)	1132
FspBI	CTAG	2 cut(s)	615, 2105
GlaI	GCGC	3 cut(s)	13, 1802, 2490
GsaI	CCCAGC	4 cut(s)	442, 1183, 2033, 2784
HaeII	RGCGCY	2 cut(s)	15, 1804
HapII	CCGG	7 cut(s)	15, 353, 516, 734, 1316, 1461, 2908
HgaI	GACGC	6 cut(s)	251, 752, 1190, 1851, 2801, 2920
HhaI	GCGC	3 cut(s)	14, 1803, 2491
Hin1I	GRCGYC	3 cut(s)	1843, 2793, 2912
Hin6I	GCGC	3 cut(s)	12, 1801, 2489
HinP1I	GCGC	3 cut(s)	12, 1801, 2489
HincII	GTYRAC	3 cut(s)	138, 1003, 2362
HindII	GTYRAC	3 cut(s)	138, 1003, 2362
HindIII	AAGCTT	2 cut(s)	1764, 1853
HinfI	GANTC	7 cut(s)	282, 491, 519, 730, 1680, 2772, 3027
HpaII	CCGG	7 cut(s)	15, 353, 516, 734, 1316, 1461, 2908
HphI	GGTGA	8 cut(s)	896, 1039, 1328, 1928, 2374, 2593, 2670, 2869
Hpy99I	CGWCG	4 cut(s)	56, 299, 302, 1206
HpyCH4IV	ACGT	1 cut(s)	99
HpyF3I	CTNAG	8 cut(s)	240, 866, 1134, 1329, 1797, 2528, 2596, 2752
HpySE526I	ACGT	1 cut(s)	99
Hsp92I	GRCGYC	3 cut(s)	1843, 2793, 2912
HspAI	GCGC	3 cut(s)	12, 1801, 2489
Kpn2I	TCCGGA	1 cut(s)	352
KpnI	GGTACC	2 cut(s)	829, 1097
Ksp22I	TGATCA	6 cut(s)	361, 565, 675, 808, 1534, 2061
LguI	GCTCTTC	3 cut(s)	66, 449, 2846
LmnI	GCTCC	9 cut(s)	230, 447, 611, 1034, 1198, 1513, 1897, 2224, 2535
Lsp1109I	GCAGC	6 cut(s)	285, 436, 553, 631, 1546, 2045
LweI	GCATC	5 cut(s)	577, 1867, 1879, 2145, 2556
MabI	ACCWGGT	1 cut(s)	2247
MaeI	CTAG	2 cut(s)	615, 2105
MaeII	ACGT	1 cut(s)	99
MbiI	CCGCTC	2 cut(s)	214, 2538
MfeI	CAATTG	3 cut(s)	642, 1881, 3031
MflI	RGATCY	7 cut(s)	524, 801, 931, 1008, 2120, 2280, 2970
MhlI	GDGCHC	4 cut(s)	78, 1132, 1742, 2266
MlsI	TGGCCA	2 cut(s)	66, 599
MluI	ACGCGT	1 cut(s)	2709
MluNI	TGGCCA	2 cut(s)	66, 599
MlyI	GAGTC	3 cut(s)	500, 528, 2781
MmeI	TCCRAC	3 cut(s)	2090, 2150, 2765
Mox20I	TGGCCA	2 cut(s)	66, 599
Mph1103I	ATGCAT	2 cut(s)	1914, 2160
MroI	TCCGGA	1 cut(s)	352
MroXI	GAANNNNTTC	2 cut(s)	2052, 2840
MscI	TGGCCA	2 cut(s)	66, 599
MseI	TTAA	7 cut(s)	339, 375, 954, 1695, 2043, 2141, 2883
MslI	CAYNNNNRTG	6 cut(s)	66, 536, 599, 1087, 2057, 2163
Msp20I	TGGCCA	2 cut(s)	66, 599
MspA1I	CMGCKG	3 cut(s)	427, 1073, 1371
MspI	CCGG	7 cut(s)	15, 353, 516, 734, 1316, 1461, 2908
MspR9I	CCNGG	6 cut(s)	1317, 1341, 1437, 1478, 2249, 2945
MunI	CAATTG	3 cut(s)	642, 1881, 3031
Mva1269I	GAATGC	4 cut(s)	1477, 1914, 2204, 2517
MvaI	CCWGG	5 cut(s)	1341, 1437, 1478, 2249, 2945
MvnI	CGCG	2 cut(s)	1667, 2711
NciI	CCSGG	1 cut(s)	1317
NcoI	CCATGG	1 cut(s)	987
NmeAIII	GCCGAG	1 cut(s)	667
NmuCI	GTSAC	8 cut(s)	492, 1364, 1820, 1916, 1981, 2125, 2665, 2676
NsiI	ATGCAT	2 cut(s)	1914, 2160
NspI	RCATGY	2 cut(s)	898, 1420
NspV	TTCGAA	1 cut(s)	1053
PaeR7I	CTCGAG	2 cut(s)	1125, 2342
PceI	AGGCCT	3 cut(s)	196, 1377, 1435
PciI	ACATGT	1 cut(s)	1416
PciSI	GCTCTTC	3 cut(s)	66, 449, 2846
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PctI	GAATGC	4 cut(s)	1477, 1914, 2204, 2517
PdmI	GAANNNNTTC	2 cut(s)	2052, 2840
PfeI	GAWTC	4 cut(s)	282, 730, 1680, 3027
PflMI	CCANNNNNTGG	1 cut(s)	2818
Ple19I	CGATCG	1 cut(s)	856
PleI	GAGTC	3 cut(s)	499, 527, 2780
PpsI	GAGTC	3 cut(s)	499, 527, 2780
PpuMI	RGGWCCY	3 cut(s)	1724, 1793, 3005
PscI	ACATGT	1 cut(s)	1416
PshBI	ATTAAT	1 cut(s)	339
PsiI	TTATAA	1 cut(s)	408
Psp5II	RGGWCCY	3 cut(s)	1724, 1793, 3005
Psp6I	CCWGG	5 cut(s)	1339, 1435, 1476, 2247, 2943
PspFI	CCCAGC	4 cut(s)	438, 1179, 2029, 2780
PspGI	CCWGG	5 cut(s)	1339, 1435, 1476, 2247, 2943
PspPI	GGNCC	7 cut(s)	470, 1177, 1588, 1724, 1793, 2822, 3005
PspPPI	RGGWCCY	3 cut(s)	1724, 1793, 3005
PspXI	VCTCGAGB	2 cut(s)	1125, 2342
PsuI	RGATCY	7 cut(s)	524, 801, 931, 1008, 2120, 2280, 2970
PvuI	CGATCG	1 cut(s)	856
PvuII	CAGCTG	1 cut(s)	427
RseI	CAYNNNNRTG	6 cut(s)	66, 536, 599, 1087, 2057, 2163
SalI	GTCGAC	1 cut(s)	1001
SapI	GCTCTTC	3 cut(s)	66, 449, 2846
SaqAI	TTAA	7 cut(s)	339, 375, 954, 1695, 2043, 2141, 2883
Sau96I	GGNCC	7 cut(s)	470, 1177, 1588, 1724, 1793, 2822, 3005
ScaI	AGTACT	3 cut(s)	925, 1497, 1865
SchI	GAGTC	3 cut(s)	500, 528, 2781
ScrFI	CCNGG	6 cut(s)	1317, 1341, 1437, 1478, 2249, 2945
SduI	GDGCHC	4 cut(s)	78, 1132, 1742, 2266
SexAI	ACCWGGT	1 cut(s)	2247
SfaNI	GCATC	5 cut(s)	577, 1867, 1879, 2145, 2556
Sfr274I	CTCGAG	2 cut(s)	1125, 2342
SfuI	TTCGAA	1 cut(s)	1053
SinI	GGWCC	4 cut(s)	1588, 1724, 1793, 3005
SlaI	CTCGAG	2 cut(s)	1125, 2342
SmiMI	CAYNNNNRTG	6 cut(s)	66, 536, 599, 1087, 2057, 2163
SmlI	CTYRAG	8 cut(s)	628, 1125, 1166, 2023, 2333, 2342, 2522, 2630
SmoI	CTYRAG	8 cut(s)	628, 1125, 1166, 2023, 2333, 2342, 2522, 2630
SseBI	AGGCCT	3 cut(s)	196, 1377, 1435
SspMI	CTAG	2 cut(s)	615, 2105
StuI	AGGCCT	3 cut(s)	196, 1377, 1435
StyD4I	CCNGG	6 cut(s)	1315, 1339, 1435, 1476, 2247, 2943
StyI	CCWWGG	4 cut(s)	553, 987, 1869, 3000
TaiI	ACGT	1 cut(s)	102
TaqII	GACCGA	1 cut(s)	1665
TatI	WGTACW	3 cut(s)	923, 1495, 1863
TauI	GCSGC	5 cut(s)	123, 392, 561, 1848, 2646
TfiI	GAWTC	4 cut(s)	282, 730, 1680, 3027
Tru1I	TTAA	7 cut(s)	339, 375, 954, 1695, 2043, 2141, 2883
Tru9I	TTAA	7 cut(s)	339, 375, 954, 1695, 2043, 2141, 2883
TscAI	CASTG	6 cut(s)	192, 248, 568, 973, 2440, 3026
TseFI	GTSAC	8 cut(s)	492, 1364, 1820, 1916, 1981, 2125, 2665, 2676
TseI	GCWGC	6 cut(s)	273, 424, 541, 619, 1559, 2033
Tsp45I	GTSAC	8 cut(s)	492, 1364, 1820, 1916, 1981, 2125, 2665, 2676
TspGWI	ACGGA	4 cut(s)	162, 434, 591, 2133
TspRI	CASTG	6 cut(s)	192, 248, 568, 973, 2440, 3026
Van91I	CCANNNNNTGG	1 cut(s)	2818
VpaK11BI	GGWCC	4 cut(s)	1588, 1724, 1793, 3005
VspI	ATTAAT	1 cut(s)	339
XapI	RAATTY	8 cut(s)	329, 1076, 1109, 1229, 1716, 1945, 1958, 2441
XceI	RCATGY	2 cut(s)	898, 1420
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1358, 1474
XhoI	CTCGAG	2 cut(s)	1125, 2342
XmiI	GTMKAC	3 cut(s)	1002, 1413, 2373
XmnI	GAANNNNTTC	2 cut(s)	2052, 2840
XspI	CTAG	2 cut(s)	615, 2105
ZrmI	AGTACT	3 cut(s)	925, 1497, 1865
Zsp2I	ATGCAT	2 cut(s)	1914, 2160

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.