MD02G1010700.v1.1

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015455
Physical Location & Seq
Forward (+)
694716 .. 696825
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1010700.v1.1.491

Sequence Viewer

Length: 519 bp
ATGTCAAGGCGATCTGCTGGCTGTTTGAGGTGCTGTTTGGTGGTGTTTGCGGTGGTTTCTGCATTGGGGGTTTGCGGGCCGGCCTTGTATTGGCGGTTTAAGAAGACCATAAAGTTGGGGGATTCTAAAATATCTTGCCCTTCTTGCAACTGCGATTGTCCTCCTCCTTTGTCTCTCCTCAAGATTGCTCCTGGATTGGCCAATCTCTCAGTCACAGATTGCGGAAGTAATGACCCGGATCTGAAGCAGGAGATGGAGAAGCAGTTCGTGGACCTTTTGACAGAGGAATTGAAGCTGCAAGAGGCCGTTGCCGCAGAGCACACCCGTCACATGAACATCACATTTGCTGAAGCAAAGAGGGTGGCTTCTCAGTACCAGAAGGAAGCAGAAAAGTGCAATGCTGCAACAGAAACTTGCGAGGAGGCGAGAGAGCGCGCTGAGGCATTGCTCATCAAGGAGAGAAAGGTGACTTTCTTGTGGGAAAGAAGAGCCCGTCAAATGGGTTGGGATAGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

173

Amino Acids

19.23

Weight (kDa)

7.99

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 7 - 170 2e-73 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30996
fragaria_vesca FvH4_1g01280
malus_domestica MD02G1010700.v1.1 MD15G1155600.v1.1
prunus_persica Prupe.7G259500_v2.0.a1
pyrus_communis pycom02g00960 pycom15g13960
rosa_chinensis RchiOBHm_Chr2g0086111
rosa_laevigata RLG00000015744
rosa_multiflora Rmu_sc0004510.1_g000001
rosa_roxburghii Rroxscaffold_2G00154800
rosa_rugosa Rorug01G0465800
rosa_samantha Rh2BG015500 Rh2CG016400 Rh2DG017500
rosa_wichuraiana Rw2G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 435
AciI CCGC 5 cut(s) 50, 75, 94, 222, 312
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 1 cut(s) 198
AcuI CTGAAG 2 cut(s) 263, 369
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 90, 499
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 190
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 246
AoxI GGCC 4 cut(s) 77, 81, 198, 303
ApeKI GCWGC 2 cut(s) 295, 401
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 435, 437
AspS9I GGNCC 2 cut(s) 77, 271
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 478
AvaII GGWCC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 282, 388
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 192
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 177
BisI GCNGC 3 cut(s) 296, 312, 402
BlsI GCNGC 3 cut(s) 297, 313, 403
Bme1390I CCNGG 2 cut(s) 192, 236
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 77, 271
BmrFI CCNGG 2 cut(s) 192, 236
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 236
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 499
Bse118I RCCGGY 1 cut(s) 79
Bse3DI GCAATG 2 cut(s) 403, 443
BseBI CCWGG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 90, 499
BseMI GCAATG 2 cut(s) 403, 443
BseMII CTCAG 3 cut(s) 222, 383, 429
BsePI GCGCGC 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 153, 167, 434
BseXI GCAGC 2 cut(s) 282, 388
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 4 cut(s) 79, 83, 200, 305
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 2 cut(s) 80, 236
BslI CCNNNNNNNGG 2 cut(s) 90, 499
BsmAI GTCTC 1 cut(s) 177
BsnI GGCC 4 cut(s) 79, 83, 200, 305
Bsp1286I GDGCHC 2 cut(s) 321, 493
Bsp143I GATC 2 cut(s) 11, 238
BspACI CCGC 5 cut(s) 50, 75, 94, 222, 312
BspANI GGCC 4 cut(s) 79, 83, 200, 305
BspCNI CTCAG 3 cut(s) 221, 382, 430
BspFNI CGCG 1 cut(s) 435
BspPI GGATC 1 cut(s) 246
BspQI GCTCTTC 1 cut(s) 481
BsrDI GCAATG 2 cut(s) 403, 443
BsrFI RCCGGY 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 79
BssHII GCGCGC 1 cut(s) 433
BssMI GATC 2 cut(s) 11, 238
Bst2UI CCWGG 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 481
BstC8I GCNNGC 4 cut(s) 19, 77, 81, 435
BstDEI CTNAG 3 cut(s) 208, 369, 438
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 2 cut(s) 435, 437
BstKTI GATC 2 cut(s) 14, 241
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 11, 238
BstMWI GCNNNNNNNGC 2 cut(s) 144, 311
BstNI CCWGG 1 cut(s) 192
BstSCI CCNGG 2 cut(s) 190, 234
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 282, 388
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 4 cut(s) 79, 83, 200, 305
Cac8I GCNNGC 4 cut(s) 19, 77, 81, 435
CfoI GCGC 2 cut(s) 435, 437
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 77, 271
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 1 cut(s) 331
CviJI RGCY 8 cut(s) 21, 79, 83, 200, 295, 305, 365, 491
CviKI_1 RGCY 8 cut(s) 21, 79, 83, 200, 295, 305, 365, 491
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 3 cut(s) 208, 369, 438
DpnI GATC 2 cut(s) 13, 240
DpnII GATC 2 cut(s) 11, 238
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 263, 369
EcoRII CCWGG 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 1 cut(s) 334
FaiI YATR 2 cut(s) 110, 332
FatI CATG 1 cut(s) 330
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 3 cut(s) 296, 312, 402
FriOI GRGCYC 1 cut(s) 493
FseI GGCCGGCC 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 296, 312, 402
GlaI GCGC 2 cut(s) 434, 436
GluI GCNGC 3 cut(s) 296, 312, 402
HaeIII GGCC 4 cut(s) 79, 83, 200, 305
HapII CCGG 2 cut(s) 80, 236
HhaI GCGC 2 cut(s) 435, 437
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 2 cut(s) 433, 435
HinP1I GCGC 2 cut(s) 433, 435
HinfI GANTC 1 cut(s) 122
HpaII CCGG 2 cut(s) 80, 236
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 150, 373
HpyCH4V TGCA 5 cut(s) 62, 147, 298, 396, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 311
HpyF3I CTNAG 3 cut(s) 208, 369, 438
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 2 cut(s) 433, 435
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 11, 238
LguI GCTCTTC 1 cut(s) 481
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 7 cut(s) 3, 93, 177, 204, 233, 249, 389
Lsp1109I GCAGC 2 cut(s) 282, 388
MaeIII GTNAC 3 cut(s) 211, 326, 466
MalI GATC 2 cut(s) 13, 240
MboI GATC 2 cut(s) 11, 238
MboII GAAGA 2 cut(s) 115, 498
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 321, 493
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 1 cut(s) 287
MluNI TGGCCA 1 cut(s) 200
Mox20I TGGCCA 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 2 cut(s) 80, 236
MspR9I CCNGG 2 cut(s) 192, 236
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 144, 311
NaeI GCCGGC 1 cut(s) 81
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 11, 238
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 211, 326, 466
PauI GCGCGC 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 481
PdiI GCCGGC 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 190
PkrI GCNGC 3 cut(s) 297, 313, 403
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspPI GGNCC 2 cut(s) 77, 271
PsuI RGATCY 1 cut(s) 238
PteI GCGCGC 1 cut(s) 433
RigI GGCCGGCC 1 cut(s) 83
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 3 cut(s) 296, 312, 402
Sau3AI GATC 2 cut(s) 11, 238
Sau96I GGNCC 2 cut(s) 77, 271
ScrFI CCNGG 2 cut(s) 192, 236
SduI GDGCHC 2 cut(s) 321, 493
SetI ASST 4 cut(s) 32, 276, 297, 468
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 1 cut(s) 287
SsiI CCGC 5 cut(s) 50, 75, 94, 222, 312
StyD4I CCNGG 2 cut(s) 190, 234
TasI AATT 1 cut(s) 287
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TseFI GTSAC 3 cut(s) 211, 326, 466
TseI GCWGC 2 cut(s) 295, 401
Tsp45I GTSAC 3 cut(s) 211, 326, 466
TspDTI ATGAA 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.