MD15G1155600.v1.1

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015455
Physical Location & Seq
Forward (+)
11573003 .. 11575339
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1155600.v1.1.491

Sequence Viewer

Length: 519 bp
ATGTCAAGACGATCTGCTACCTGTTTGAGGTGCTGTTTGGTGGTGTTTGCGGTGGTTTCTGCATTGGGGGTTTGCGGGCCGGCCTTGTATTGGCGGTTCAAGAAGACCGTAAAGTTGGGGGGTTCTAAAATCTCTTGCCCTTCTTGCAACTGCGATTGCCCTCCTCCTTTGTCTCTCCTCAAGATTGCTCCTGGATTGGCCAATCTCTCAGTCACAGATTGCGGAAGTAATGACCCAGATCTGAAGCGGGAGATGGAGAAGCAATTTGTGGACCTTTTAACAGAGGAATTGAAGCTGCAAGAGGCTGTTGCTGCAGAGCACACCCGACACATGAACATCACATGTGCTGAAGCAAAGAGGGTGGCTTCTCAGTACCAGAAAGAAGCAGAAAAGTGCAATGCTGCAACAGAAACTTGCGAGGAGGCGAGAGAGCGCGCTGAGGCATTGCTCATCAAGGAGAGAAAGGTGACTTTCTTGTGGGAAAGACGAGCCCGTCAAATGGGTTGGGCAGGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

173

Amino Acids

19.13

Weight (kDa)

8.45

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 7 - 170 2.8e-72 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30996
fragaria_vesca FvH4_1g01280
malus_domestica MD02G1010700.v1.1 MD15G1155600.v1.1
prunus_persica Prupe.7G259500_v2.0.a1
pyrus_communis pycom02g00960 pycom15g13960
rosa_chinensis RchiOBHm_Chr2g0086111
rosa_laevigata RLG00000015744
rosa_multiflora Rmu_sc0004510.1_g000001
rosa_roxburghii Rroxscaffold_2G00154800
rosa_rugosa Rorug01G0465800
rosa_samantha Rh2BG015500 Rh2CG016400 Rh2DG017500
rosa_wichuraiana Rw2G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 492
AccII CGCG 1 cut(s) 435
AciI CCGC 5 cut(s) 50, 75, 94, 222, 247
AcoI YGGCCR 1 cut(s) 198
AcuI CTGAAG 2 cut(s) 263, 369
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 27, 90, 499
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 2 cut(s) 100, 292
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 177
AoxI GGCC 3 cut(s) 77, 81, 198
ApeKI GCWGC 3 cut(s) 295, 311, 401
AspLEI GCGC 2 cut(s) 435, 437
AspS9I GGNCC 2 cut(s) 77, 271
AsuHPI GGTGA 1 cut(s) 478
AvaII GGWCC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 282, 298, 388
BccI CCATC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 312
BglII AGATCT 1 cut(s) 238
BisI GCNGC 3 cut(s) 296, 312, 402
BlsI GCNGC 3 cut(s) 297, 313, 403
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 77, 271
BmrFI CCNGG 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 90, 499
Bse118I RCCGGY 1 cut(s) 79
Bse3DI GCAATG 2 cut(s) 403, 443
BseBI CCWGG 1 cut(s) 192
BseLI CCNNNNNNNGG 3 cut(s) 27, 90, 499
BseMI GCAATG 2 cut(s) 403, 443
BseMII CTCAG 3 cut(s) 222, 383, 429
BsePI GCGCGC 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 153, 167, 434
BseXI GCAGC 3 cut(s) 282, 298, 388
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 79, 83, 200
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 27, 90, 499
BsmAI GTCTC 1 cut(s) 177
BsnI GGCC 3 cut(s) 79, 83, 200
Bsp1286I GDGCHC 2 cut(s) 321, 493
Bsp143I GATC 2 cut(s) 11, 238
BspACI CCGC 5 cut(s) 50, 75, 94, 222, 247
BspANI GGCC 3 cut(s) 79, 83, 200
BspCNI CTCAG 3 cut(s) 221, 382, 430
BspFNI CGCG 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 316
BsrDI GCAATG 2 cut(s) 403, 443
BsrFI RCCGGY 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 79
BssHII GCGCGC 1 cut(s) 433
BssMI GATC 2 cut(s) 11, 238
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 77, 81, 435
BstDEI CTNAG 3 cut(s) 208, 369, 438
BstENI CCTNNNNNAGG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 2 cut(s) 435, 437
BstKTI GATC 2 cut(s) 14, 241
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 11, 238
BstMWI GCNNNNNNNGC 2 cut(s) 144, 311
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 282, 298, 388
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 3 cut(s) 79, 83, 200
Cac8I GCNNGC 3 cut(s) 77, 81, 435
CfoI GCGC 2 cut(s) 435, 437
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 77, 271
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 2 cut(s) 331, 342
CviJI RGCY 7 cut(s) 79, 83, 200, 295, 305, 365, 491
CviKI_1 RGCY 7 cut(s) 79, 83, 200, 295, 305, 365, 491
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 3 cut(s) 208, 369, 438
DpnI GATC 2 cut(s) 13, 240
DpnII GATC 2 cut(s) 11, 238
DrdI GACNNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 263, 369
EcoNI CCTNNNNNAGG 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 2 cut(s) 334, 345
FaiI YATR 2 cut(s) 332, 343
FatI CATG 2 cut(s) 330, 341
FauI CCCGC 2 cut(s) 68, 240
Fnu4HI GCNGC 3 cut(s) 296, 312, 402
FriOI GRGCYC 1 cut(s) 493
FseI GGCCGGCC 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 296, 312, 402
GlaI GCGC 2 cut(s) 434, 436
GluI GCNGC 3 cut(s) 296, 312, 402
HaeIII GGCC 3 cut(s) 79, 83, 200
HapII CCGG 1 cut(s) 80
HhaI GCGC 2 cut(s) 435, 437
Hin1II CATG 2 cut(s) 334, 345
Hin6I GCGC 2 cut(s) 433, 435
HinP1I GCGC 2 cut(s) 433, 435
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 3 cut(s) 6, 100, 181
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 6 cut(s) 62, 147, 298, 314, 396, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 311
HpyF3I CTNAG 3 cut(s) 208, 369, 438
Hsp92II CATG 2 cut(s) 334, 345
HspAI GCGC 2 cut(s) 433, 435
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 11, 238
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 7 cut(s) 34, 93, 177, 204, 249, 389, 495
Lsp1109I GCAGC 3 cut(s) 282, 298, 388
MaeIII GTNAC 2 cut(s) 211, 466
MalI GATC 2 cut(s) 13, 240
MboI GATC 2 cut(s) 11, 238
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 321, 493
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 2 cut(s) 263, 287
MluNI TGGCCA 1 cut(s) 200
Mox20I TGGCCA 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 79
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 278
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 144, 311
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 2 cut(s) 11, 238
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 2 cut(s) 334, 345
NmuCI GTSAC 2 cut(s) 211, 466
NspI RCATGY 1 cut(s) 345
PauI GCGCGC 1 cut(s) 433
PciI ACATGT 1 cut(s) 341
PdiI GCCGGC 1 cut(s) 81
PfoI TCCNGGA 1 cut(s) 190
PkrI GCNGC 3 cut(s) 297, 313, 403
PscI ACATGT 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspPI GGNCC 2 cut(s) 77, 271
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstI CTGCAG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 238
PteI GCGCGC 1 cut(s) 433
RigI GGCCGGCC 1 cut(s) 83
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 3 cut(s) 296, 312, 402
Sau3AI GATC 2 cut(s) 11, 238
Sau96I GGNCC 2 cut(s) 77, 271
ScrFI CCNGG 1 cut(s) 192
SduI GDGCHC 2 cut(s) 321, 493
SetI ASST 5 cut(s) 23, 32, 276, 297, 468
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 2 cut(s) 263, 287
SsiI CCGC 5 cut(s) 50, 75, 94, 222, 247
StyD4I CCNGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 109
TasI AATT 2 cut(s) 263, 287
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseFI GTSAC 2 cut(s) 211, 466
TseI GCWGC 3 cut(s) 295, 311, 401
Tsp45I GTSAC 2 cut(s) 211, 466
TspDTI ATGAA 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 25
XceI RCATGY 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.