MD02G1034100.v1.1

Adenine nucleotide transporter BT1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014630
Physical Location & Seq
Forward (+)
2624655 .. 2626430
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1034100.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGATAGGAGAAGATTGCAGGCCACTGAAAATACAAACGACGATGTAGTATTCTCTTGTTCTTCCGGGATTAGAATCCGACGCAGTCCTCTAAATAGCACTTCGCAATCCGGTGGACTGTTTGCCAGCGTTGGACAAGTTGCAGGAGGATTTGGTATCACACCAAGTCCACAGAACTCCATAAACCAAACCACAAAGCTTCTGAGCTCCACGTACATGACATACATGCCCACGCCGGACTTCGGGCTGCGAATTAGTAGGGTTGACGAGTTGGTGGAGGCAGAGTCACTGTTCGAGGTTGGAGAAGCAGAAGAGCTGCTGAAAGTCAACAAAATCAAAGGTAATGGTGGTGGTTTGAGGTTGAAAATAAAGGTTGGGAATGCATCACTGAGGAGGCTGGTGAGTGGAGCGGTGGCGGGAGTGGTGTCGCGGATGGCGGTTGCGCCGCTGGAGACGATACGGACGCATTTGATGGTGGGGGGCAGTGGCCACTCCAGTGCTTCTCATGTCTTTCAGTCTATCATGGAAACTGATGGATGGCAGGGCTTGTTCAGGGGTACTTTGGTCAATGTCATCAGGGTTGCTCCAAACAAGGCTATTGAGTTATTTGCTTATGACACGGTGAAGAAGCACTTAACTCGACCCGAAGAAGACCTGAAAATCCCTATCCCGGCTTCATCAATCGCAGGCGCTGTTGCCGGATTTAGCTCAACCTTGTGTACATACCCTCTCGAGTTGTTAAAAACACGATTAACAGTCCAGAAAGGAGCGTACAACAATTTTTTCGACGCAGTTTCGAAAATTGTCCAACAGGAGGGAGCTGCAGGGCTATACAGAGGCCTCACCCCGAGTTTAATCGGCGTAATTCCATACGCTGCCTCCAACTATTTTGCTTACGATGCGCTCTCAAGAGCTTACAAGAAAGCTTTCAAAAAGGAGGAAATCGGAAACATAGCAACTCTTTTGATCGGATCAGCAGCCGCTGCAATTTCGAGCAGTGCAACTTTCCCGCTTGAGGTGGCTCGGAAGAGAATGCAGGTTGGGGCTCTCGGTGGGAGGCAATACAAGCACATACTTGATGCTCTTTCGAGCATACTTGCCAGTGAAGGAATCAGTGGCCTGTACAGAGGGTTGGGGCCGAGCTACATGAAATTGGTCCCTGCTGCTGGGATTTCCTTCATGTGCTACGAGGCTTGCAAGCGGATACTGAATGAGAACGATGAAGAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

411

Amino Acids

44.0

Weight (kDa)

9.33

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 130 - 214 2.6e-22 Mitochondrial carrier protein
Mito_carr PF00153 222 - 308 4e-24 Mitochondrial carrier protein
Mito_carr PF00153 317 - 406 2.9e-24 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014630)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03510
malus_domestica MD02G1034100.v1.1 MD15G1174900.v1.1
prunus_persica Prupe.7G241000_v2.0.a1
pyrus_communis pycom02g02790 pycom15g15640
rosa_chinensis RchiOBHm_Chr2g0088721
rosa_laevigata RLG00000015979
rosa_multiflora Rmu_sc0006835.1_g000004
rosa_roxburghii Rroxscaffold_2G00152370
rosa_rugosa Rorug01G0486500 Rorug01G0486600
rosa_samantha Rh2AG041700 Rh2BG040400 Rh2CG041200 Rh2DG040800
rosa_wichuraiana Rw2G003270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1027
AccBSI CCGCTC 1 cut(s) 410
AccII CGCG 1 cut(s) 430
AciI CCGC 8 cut(s) 410, 416, 430, 437, 446, 981, 1010, 1201
AclWI GGATC 1 cut(s) 979
AcoI YGGCCR 1 cut(s) 487
AfaI GTAC 5 cut(s) 215, 559, 721, 773, 1124
AfiI CCNNNNNNNGG 5 cut(s) 242, 670, 814, 1015, 1166
AgsI TTSAA 2 cut(s) 364, 931
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 8 cut(s) 199, 207, 316, 708, 821, 914, 926, 1143
AluI AGCT 8 cut(s) 199, 207, 316, 708, 821, 914, 926, 1143
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 1 cut(s) 979
AlwNI CAGNNNCTG 2 cut(s) 692, 983
Ama87I CYCGRG 2 cut(s) 731, 847
AoxI GGCC 5 cut(s) 21, 487, 838, 1117, 1136
ApeKI GCWGC 7 cut(s) 247, 316, 821, 875, 977, 983, 1163
Asp700I GAANNNNTTC 1 cut(s) 926
AspLEI GCGC 3 cut(s) 445, 692, 904
AspS9I GGNCC 2 cut(s) 1136, 1156
AsuC2I CCSGG 2 cut(s) 67, 671
AsuHPI GGTGA 3 cut(s) 412, 634, 835
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 2 cut(s) 731, 847
AvaII GGWCC 1 cut(s) 1156
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BalI TGGCCA 1 cut(s) 489
BanII GRGCYC 2 cut(s) 209, 1048
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 7 cut(s) 234, 303, 808, 862, 970, 989, 1150
BccI CCATC 4 cut(s) 427, 466, 527, 531
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciVI GTATCC 1 cut(s) 1197
BcnI CCSGG 2 cut(s) 67, 671
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 1 cut(s) 822
BfoI RGCGCY 1 cut(s) 693
BfuAI ACCTGC 1 cut(s) 1027
BfuI GTATCC 1 cut(s) 1197
BisI GCNGC 9 cut(s) 248, 317, 446, 822, 876, 978, 981, 984, 1164
BlsI GCNGC 9 cut(s) 249, 318, 447, 823, 877, 979, 982, 985, 1165
Bme1390I CCNGG 2 cut(s) 67, 671
Bme18I GGWCC 1 cut(s) 1156
BmeT110I CYCGRG 2 cut(s) 731, 847
BmgT120I GGNCC 2 cut(s) 1136, 1156
BmiI GGNNCC 2 cut(s) 1137, 1158
BmrFI CCNGG 2 cut(s) 67, 671
BmsI GCATC 3 cut(s) 392, 889, 1069
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 2 cut(s) 470, 478
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 2 cut(s) 892, 1034
BpuMI CCSGG 2 cut(s) 67, 671
BsaAI YACGTR 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 863, 893
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 670, 814, 1015, 1166
Bse1I ACTGG 2 cut(s) 495, 1101
Bse8I GATNNNNATC 1 cut(s) 74
BseGI GGATG 2 cut(s) 438, 542
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 5 cut(s) 242, 670, 814, 1015, 1166
BseMII CTCAG 2 cut(s) 194, 380
BseNI ACTGG 2 cut(s) 495, 1101
BseRI GAGGAG 1 cut(s) 406
BseXI GCAGC 7 cut(s) 234, 303, 808, 862, 970, 989, 1150
BseYI CCCAGC 1 cut(s) 1166
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 5 cut(s) 23, 489, 840, 1119, 1138
BsiHKAI GWGCWC 1 cut(s) 209
BsiHKCI CYCGRG 2 cut(s) 731, 847
BsiSI CCGG 5 cut(s) 66, 111, 236, 671, 699
BslFI GGGAC 1 cut(s) 1142
BslI CCNNNNNNNGG 5 cut(s) 242, 670, 814, 1015, 1166
BsmAI GTCTC 1 cut(s) 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 1142
BsmI GAATGC 2 cut(s) 385, 1038
BsnI GGCC 5 cut(s) 23, 489, 840, 1119, 1138
BsoBI CYCGRG 2 cut(s) 731, 847
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 2 cut(s) 209, 1048
Bsp1407I TGTACA 2 cut(s) 719, 1122
Bsp143I GATC 2 cut(s) 966, 971
BspACI CCGC 8 cut(s) 410, 416, 430, 437, 446, 981, 1010, 1201
BspANI GGCC 5 cut(s) 23, 489, 840, 1119, 1138
BspCNI CTCAG 2 cut(s) 195, 381
BspFNI CGCG 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 1137, 1158
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 1027
BspPI GGATC 1 cut(s) 979
BspQI GCTCTTC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 797
BsrBI CCGCTC 1 cut(s) 410
BsrGI TGTACA 2 cut(s) 719, 1122
BsrI ACTGG 2 cut(s) 495, 1101
BssMI GATC 2 cut(s) 966, 971
Bst4CI ACNGT 4 cut(s) 120, 291, 622, 757
Bst6I CTCTTC 2 cut(s) 306, 1022
BstAPI GCANNNNNTGC 1 cut(s) 983
BstAUI TGTACA 2 cut(s) 719, 1122
BstBAI YACGTR 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 5 cut(s) 21, 127, 688, 1195, 1199
BstDEI CTNAG 2 cut(s) 203, 389
BstF5I GGATG 2 cut(s) 438, 542
BstFNI CGCG 1 cut(s) 430
BstH2I RGCGCY 1 cut(s) 693
BstHHI GCGC 3 cut(s) 445, 692, 904
BstKTI GATC 2 cut(s) 969, 974
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 2 cut(s) 966, 971
BstMWI GCNNNNNNNGC 3 cut(s) 899, 983, 1066
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 65, 669
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 7 cut(s) 234, 303, 808, 862, 970, 989, 1150
BstV2I GAAGAC 1 cut(s) 657
BsuI GTATCC 1 cut(s) 1197
BsuRI GGCC 5 cut(s) 23, 489, 840, 1119, 1138
BtsCI GGATG 2 cut(s) 438, 542
BtsI GCAGTG 2 cut(s) 490, 1003
BtsIMutI CAGTG 8 cut(s) 24, 287, 386, 490, 502, 1003, 1108, 1120
BveI ACCTGC 1 cut(s) 1027
Cac8I GCNNGC 5 cut(s) 21, 127, 688, 1195, 1199
CaiI CAGNNNCTG 2 cut(s) 692, 983
CfoI GCGC 3 cut(s) 445, 692, 904
Cfr13I GGNCC 2 cut(s) 1136, 1156
CseI GACGC 3 cut(s) 90, 472, 797
Csp6I GTAC 5 cut(s) 214, 558, 720, 772, 1123
CviAII CATG 6 cut(s) 217, 226, 506, 523, 1147, 1180
CviQI GTAC 5 cut(s) 214, 558, 720, 772, 1123
DdeI CTNAG 2 cut(s) 203, 389
DpnI GATC 2 cut(s) 968, 973
DpnII GATC 2 cut(s) 966, 971
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 306, 1022
EarI CTCTTC 2 cut(s) 306, 1022
Ecl136II GAGCTC 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 840
Eco24I GRGCYC 2 cut(s) 209, 1048
Eco47I GGWCC 1 cut(s) 1156
Eco53kI GAGCTC 1 cut(s) 207
Eco88I CYCGRG 2 cut(s) 731, 847
EcoICRI GAGCTC 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 385
EcoT38I GRGCYC 2 cut(s) 209, 1048
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 6 cut(s) 220, 229, 509, 526, 1150, 1183
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 1142
FatI CATG 6 cut(s) 216, 225, 505, 522, 1146, 1179
FauI CCCGC 2 cut(s) 409, 1017
Fnu4HI GCNGC 9 cut(s) 248, 317, 446, 822, 876, 978, 981, 984, 1164
FokI GGATG 2 cut(s) 445, 549
FriOI GRGCYC 2 cut(s) 209, 1048
Fsp4HI GCNGC 9 cut(s) 248, 317, 446, 822, 876, 978, 981, 984, 1164
GlaI GCGC 3 cut(s) 444, 691, 903
GluI GCNGC 9 cut(s) 248, 317, 446, 822, 876, 978, 981, 984, 1164
GsaI CCCAGC 1 cut(s) 1170
GsuI CTGGAG 2 cut(s) 470, 478
HaeII RGCGCY 1 cut(s) 693
HaeIII GGCC 5 cut(s) 23, 489, 840, 1119, 1138
HapII CCGG 5 cut(s) 66, 111, 236, 671, 699
HgaI GACGC 3 cut(s) 90, 472, 797
HhaI GCGC 3 cut(s) 445, 692, 904
Hin1II CATG 6 cut(s) 220, 229, 509, 526, 1150, 1183
Hin6I GCGC 3 cut(s) 443, 690, 902
HinP1I GCGC 3 cut(s) 443, 690, 902
HincII GTYRAC 2 cut(s) 265, 328
HindII GTYRAC 2 cut(s) 265, 328
HindIII AAGCTT 2 cut(s) 197, 924
HinfI GANTC 3 cut(s) 75, 284, 1110
HpaII CCGG 5 cut(s) 66, 111, 236, 671, 699
HphI GGTGA 3 cut(s) 412, 634, 835
Hpy166II GTNNAC 5 cut(s) 116, 170, 265, 328, 720
Hpy188I TCNGA 5 cut(s) 80, 204, 947, 971, 1026
Hpy188III TCNNGA 3 cut(s) 731, 760, 909
Hpy8I GTNNAC 5 cut(s) 116, 170, 265, 328, 720
Hpy99I CGWCG 3 cut(s) 44, 84, 791
HpyAV CCTTC 2 cut(s) 1100, 1186
HpyCH4III ACNGT 4 cut(s) 120, 291, 622, 757
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 8 cut(s) 19, 143, 383, 824, 986, 1001, 1036, 1197
HpyF10VI GCNNNNNNNGC 3 cut(s) 899, 983, 1066
HpyF3I CTNAG 2 cut(s) 203, 389
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 6 cut(s) 220, 229, 509, 526, 1150, 1183
HspAI GCGC 3 cut(s) 443, 690, 902
Kzo9I GATC 2 cut(s) 966, 971
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 5 cut(s) 212, 407, 589, 767, 818
Lsp1109I GCAGC 7 cut(s) 234, 303, 808, 862, 970, 989, 1150
LweI GCATC 3 cut(s) 392, 889, 1069
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 285
MalI GATC 2 cut(s) 968, 973
MbiI CCGCTC 1 cut(s) 410
MboI GATC 2 cut(s) 966, 971
MboII GAAGA 7 cut(s) 24, 54, 323, 637, 659, 662, 1039
MhlI GDGCHC 2 cut(s) 209, 1048
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 6 cut(s) 252, 778, 801, 864, 987, 1151
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 5 cut(s) 103, 112, 280, 832, 906
Mox20I TGGCCA 1 cut(s) 489
Mph1103I ATGCAT 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 926
MscI TGGCCA 1 cut(s) 489
MseI TTAA 4 cut(s) 635, 740, 752, 854
MslI CAYNNNNRTG 2 cut(s) 215, 495
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 448, 983
MspI CCGG 5 cut(s) 66, 111, 236, 671, 699
MspR9I CCNGG 2 cut(s) 67, 671
Mva1269I GAATGC 2 cut(s) 385, 1038
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 3 cut(s) 899, 983, 1066
NciI CCSGG 2 cut(s) 67, 671
NdeII GATC 2 cut(s) 966, 971
NlaIII CATG 6 cut(s) 220, 229, 509, 526, 1150, 1183
NlaIV GGNNCC 2 cut(s) 1137, 1158
NmeAIII GCCGAG 1 cut(s) 1164
NmuCI GTSAC 1 cut(s) 285
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 229
NspV TTCGAA 1 cut(s) 797
OliI CACNNNNGTG 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 731
PceI AGGCCT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 2 cut(s) 385, 1038
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 2 cut(s) 75, 1110
PflFI GACNNNGTC 1 cut(s) 84
PfoI TCCNGGA 1 cut(s) 65
PkrI GCNGC 9 cut(s) 249, 318, 447, 823, 877, 979, 982, 985, 1165
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 1166
PspN4I GGNNCC 2 cut(s) 1137, 1158
PspPI GGNCC 2 cut(s) 1136, 1156
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 2 cut(s) 692, 983
PsyI GACNNNGTC 1 cut(s) 84
RsaI GTAC 5 cut(s) 215, 559, 721, 773, 1124
RsaNI GTAC 5 cut(s) 214, 558, 720, 772, 1123
RseI CAYNNNNRTG 2 cut(s) 215, 495
SacI GAGCTC 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 4 cut(s) 635, 740, 752, 854
SatI GCNGC 9 cut(s) 248, 317, 446, 822, 876, 978, 981, 984, 1164
Sau3AI GATC 2 cut(s) 966, 971
Sau96I GGNCC 2 cut(s) 1136, 1156
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 67, 671
SduI GDGCHC 2 cut(s) 209, 1048
SfaNI GCATC 3 cut(s) 392, 889, 1069
SfcI CTRYAG 1 cut(s) 822
Sfr274I CTCGAG 1 cut(s) 731
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 1 cut(s) 1156
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 2 cut(s) 215, 495
SmlI CTYRAG 3 cut(s) 731, 907, 1013
SmoI CTYRAG 3 cut(s) 731, 907, 1013
Sse9I AATT 6 cut(s) 252, 778, 801, 864, 987, 1151
SseBI AGGCCT 1 cut(s) 840
SsiI CCGC 8 cut(s) 410, 416, 430, 437, 446, 981, 1010, 1201
SstI GAGCTC 1 cut(s) 209
StuI AGGCCT 1 cut(s) 840
StyD4I CCNGG 2 cut(s) 65, 669
TaaI ACNGT 4 cut(s) 120, 291, 622, 757
TaiI ACGT 1 cut(s) 215
TaqI TCGA 7 cut(s) 294, 640, 732, 786, 797, 992, 1088
TasI AATT 6 cut(s) 252, 778, 801, 864, 987, 1151
TatI WGTACW 2 cut(s) 719, 1122
TauI GCSGC 2 cut(s) 448, 983
TfiI GAWTC 2 cut(s) 75, 1110
Tru1I TTAA 4 cut(s) 635, 740, 752, 854
Tru9I TTAA 4 cut(s) 635, 740, 752, 854
TscAI CASTG 8 cut(s) 31, 294, 393, 490, 502, 1003, 1108, 1120
TseFI GTSAC 1 cut(s) 285
TseI GCWGC 7 cut(s) 247, 316, 821, 875, 977, 983, 1163
Tsp45I GTSAC 1 cut(s) 285
TspDTI ATGAA 3 cut(s) 666, 1163, 1168
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 8 cut(s) 31, 294, 393, 490, 502, 1003, 1108, 1120
Tth111I GACNNNGTC 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 1156
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 926
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.