MD15G1174900.v1.1

Adenine nucleotide transporter BT1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014630
Physical Location & Seq
Forward (+)
13610278 .. 13611840
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1174900.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGGTGGAAGAAGATTGCAGGGTACTGGAAATACAAACGACGATGTAGTATTCTCTTGTTCTTCCGGGATTAGAATCCGATGCAGTCCTCTAAATAATACTTCGCAGTCCGGTGGACTATTTGCCAGCGTTCAAGTTGCAAGAGGATTTGGTATCACACCGAGTCCTCAGAACTCCATAGACCAAACCACAAAGCTTCTAAGCTCCACGTGCATGACATACATGTCGACGCCGGGTTTCGGGTTTTGGATTGGCAGGGTTAATGAGTTGGTGGAGGCAGATGCATTGCTGGAGGTTGCAGAAGCAGAAGAGCTGTTGAAGATTCAGAAAATTAATGGTAACGGTGGTGGTTTGAGATTGAAAATAAAGGTTGGACATGCATCGCTGAGGCGGCTGCTGAGCGGAGCAGTGGCGGGAGTGGTGTCGCGGACGGCGGTTGCTCCATTGGAGACGATACGGCCGCATTTGATGGTGGAGGGCAGTGGCCACTCCAGTGCTTCTCAAGTGTTTCATTCTATCATGGAAGCTGATGGATGGCAGGGCTTGTTTAGGGGTACTTTGGTCAATGTCATCAGGGTTGCTCCATGCAAGGCTATTGAGTTATTTGCTTATGACACGGTGAAGAAGCACTTAACTCGACCCGGTGAAGAGCTGAAAATCCCTATCCCCGCTTCATCAATCGCAGGCGCTGTTGCTGGATTCAGCTCAACCTTGTGTACATACCCTCTAGAGTTGTTAAAAACACGATTAACCATCCAGAGAGGAGTGTACAATAACTTTTTCGACGTAGTTTCAAAAATTATCCGACAGGAGGGACCTGCAGGGCTATACAGAGGACTCACCCCGAGTTTAATTGGCGTAATTCCATACGCTGCCGCCAACTATTTTGCTTATGATGCCCTCTCAAAAGCATACAAGAAAGCTTTCAAAAAGGAGGAAATCGGAAACATAACGACTCTCTTGATCGGATCAGCAGCTGCTGCAATTTCTAGCAGCGCAACTTTCCCGCTTGAGGTGGCTCGGAAGAGAATGCAGGTTGGGGCTCTCAGTGGGAGGCAGTACAAGCACATACTTGATGCTCTTTCAAGCATACATGCGAGTGAAGGAATCGGTGGCCTGTACAAAGGGTTAGGGCCGAGCTACATGAAATTTGTCCCAGCTGCTGGGATTTCCTTCATGTGCTATGAAGCATGCAAGCGAATACTGACTGAGAACGAGGAAGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

410

Amino Acids

43.8

Weight (kDa)

9.28

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 129 - 213 9.2e-21 Mitochondrial carrier protein
Mito_carr PF00153 221 - 307 1.7e-24 Mitochondrial carrier protein
Mito_carr PF00153 316 - 405 2e-23 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014630)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03510
malus_domestica MD02G1034100.v1.1 MD15G1174900.v1.1
prunus_persica Prupe.7G241000_v2.0.a1
pyrus_communis pycom02g02790 pycom15g15640
rosa_chinensis RchiOBHm_Chr2g0088721
rosa_laevigata RLG00000015979
rosa_multiflora Rmu_sc0006835.1_g000004
rosa_roxburghii Rroxscaffold_2G00152370
rosa_rugosa Rorug01G0486500 Rorug01G0486600
rosa_samantha Rh2AG041700 Rh2BG040400 Rh2CG041200 Rh2DG040800
rosa_wichuraiana Rw2G003270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 223
Acc36I ACCTGC 2 cut(s) 826, 1024
AccB7I CCANNNNNTGG 1 cut(s) 1163
AccBSI CCGCTC 1 cut(s) 402
AccI GTMKAC 1 cut(s) 227
AccII CGCG 1 cut(s) 427
AciI CCGC 9 cut(s) 391, 402, 413, 427, 434, 461, 669, 876, 1007
AclWI GGATC 1 cut(s) 976
AcoI YGGCCR 2 cut(s) 458, 484
AcsI RAATTY 1 cut(s) 1148
AcvI CACGTG 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 6 cut(s) 25, 556, 718, 770, 1061, 1121
AfiI CCNNNNNNNGG 4 cut(s) 239, 811, 1012, 1163
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 6 cut(s) 134, 319, 361, 795, 928, 1086
AleI CACNNNNGTG 1 cut(s) 492
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 976
AlwNI CAGNNNCTG 4 cut(s) 689, 977, 980, 1163
Ama87I CYCGRG 1 cut(s) 844
AoxI GGCC 4 cut(s) 458, 484, 1114, 1133
ApeKI GCWGC 7 cut(s) 394, 872, 974, 977, 980, 993, 1160
ApoI RAATTY 1 cut(s) 1148
AseI ATTAAT 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 923
AspLEI GCGC 2 cut(s) 689, 998
AspS9I GGNCC 2 cut(s) 815, 1133
AsuC2I CCSGG 3 cut(s) 67, 234, 642
AsuHPI GGTGA 3 cut(s) 631, 656, 832
AvaI CYCGRG 1 cut(s) 844
AvaII GGWCC 1 cut(s) 815
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BalI TGGCCA 1 cut(s) 486
BanII GRGCYC 1 cut(s) 1045
BbrPI CACGTG 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 7 cut(s) 381, 859, 964, 967, 986, 1005, 1147
BccI CCATC 4 cut(s) 463, 524, 528, 761
BceAI ACGGC 2 cut(s) 447, 473
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BcnI CCSGG 3 cut(s) 67, 234, 642
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 728, 990
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 690
BfuAI ACCTGC 2 cut(s) 826, 1024
BlpI GCTNAGC 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 67, 234, 642
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 844
BmgT120I GGNCC 2 cut(s) 815, 1133
BmiI GGNNCC 1 cut(s) 816
BmrFI CCNGG 3 cut(s) 67, 234, 642
BmsI GCATC 5 cut(s) 71, 271, 389, 886, 1066
BpmI CTGGAG 2 cut(s) 311, 475
Bpu10I CCTNAGC 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 398
BpuEI CTTGAG 2 cut(s) 486, 1031
BpuMI CCSGG 3 cut(s) 67, 234, 642
BsaAI YACGTR 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 811, 1012, 1163
Bse1I ACTGG 2 cut(s) 31, 492
Bse3DI GCAATG 1 cut(s) 284
Bse8I GATNNNNATC 1 cut(s) 74
BseGI GGATG 2 cut(s) 539, 753
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 4 cut(s) 239, 811, 1012, 1163
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 5 cut(s) 182, 377, 389, 1060, 1200
BseNI ACTGG 2 cut(s) 31, 492
BseRI GAGGAG 1 cut(s) 777
BseX3I CGGCCG 1 cut(s) 458
BseXI GCAGC 7 cut(s) 381, 859, 964, 967, 986, 1005, 1147
BseYI CCCAGC 2 cut(s) 1156, 1163
Bsh1236I CGCG 1 cut(s) 427
Bsh1285I CGRYCG 1 cut(s) 461
BshFI GGCC 4 cut(s) 460, 486, 1116, 1135
BsiEI CGRYCG 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 844
BsiSI CCGG 4 cut(s) 66, 111, 233, 642
BslFI GGGAC 2 cut(s) 828, 1139
BslI CCNNNNNNNGG 4 cut(s) 239, 811, 1012, 1163
BsmAI GTCTC 1 cut(s) 443
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 2 cut(s) 828, 1139
BsmI GAATGC 1 cut(s) 1035
BsnI GGCC 4 cut(s) 460, 486, 1116, 1135
BsoBI CYCGRG 1 cut(s) 844
Bsp1286I GDGCHC 1 cut(s) 1045
Bsp1407I TGTACA 3 cut(s) 716, 768, 1119
Bsp143I GATC 2 cut(s) 963, 968
Bsp1720I GCTNAGC 1 cut(s) 398
BspACI CCGC 9 cut(s) 391, 402, 413, 427, 434, 461, 669, 876, 1007
BspANI GGCC 4 cut(s) 460, 486, 1116, 1135
BspCNI CTCAG 5 cut(s) 181, 378, 390, 1059, 1201
BspFNI CGCG 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 816
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 2 cut(s) 826, 1024
BspPI GGATC 1 cut(s) 976
BspQI GCTCTTC 2 cut(s) 303, 642
BsrBI CCGCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 284
BsrGI TGTACA 3 cut(s) 716, 768, 1119
BsrI ACTGG 2 cut(s) 31, 492
BssMI GATC 2 cut(s) 963, 968
BssNI GRCGYC 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 344, 619
Bst6I CTCTTC 3 cut(s) 303, 642, 1019
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 980
BstAUI TGTACA 3 cut(s) 716, 768, 1119
BstBAI YACGTR 1 cut(s) 210
BstC8I GCNNGC 4 cut(s) 127, 685, 1192, 1196
BstDEI CTNAG 6 cut(s) 168, 200, 386, 398, 1046, 1209
BstF5I GGATG 2 cut(s) 539, 753
BstFNI CGCG 1 cut(s) 427
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 2 cut(s) 689, 998
BstKTI GATC 2 cut(s) 966, 971
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 963, 968
BstMCI CGRYCG 1 cut(s) 461
BstMWI GCNNNNNNNGC 5 cut(s) 210, 391, 896, 980, 1063
BstNSI RCATGY 4 cut(s) 226, 380, 1097, 1194
BstSCI CCNGG 3 cut(s) 65, 232, 640
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 427
BstV1I GCAGC 7 cut(s) 381, 859, 964, 967, 986, 1005, 1147
BstZI CGGCCG 1 cut(s) 458
BsuRI GGCC 4 cut(s) 460, 486, 1116, 1135
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 2 cut(s) 539, 753
BtsI GCAGTG 2 cut(s) 414, 487
BtsIMutI CAGTG 4 cut(s) 414, 487, 499, 1054
BveI ACCTGC 2 cut(s) 826, 1024
Cac8I GCNNGC 4 cut(s) 127, 685, 1192, 1196
CaiI CAGNNNCTG 4 cut(s) 689, 977, 980, 1163
CfoI GCGC 2 cut(s) 689, 998
Cfr13I GGNCC 2 cut(s) 815, 1133
CseI GACGC 1 cut(s) 238
Csp6I GTAC 6 cut(s) 24, 555, 717, 769, 1060, 1120
CviAII CATG 9 cut(s) 214, 223, 377, 520, 585, 1094, 1144, 1177, 1191
CviQI GTAC 6 cut(s) 24, 555, 717, 769, 1060, 1120
DdeI CTNAG 6 cut(s) 168, 200, 386, 398, 1046, 1209
DpnI GATC 2 cut(s) 965, 970
DpnII GATC 2 cut(s) 963, 968
DrdI GACNNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 458, 484
EagI CGGCCG 1 cut(s) 458
Eam1104I CTCTTC 3 cut(s) 303, 642, 1019
EarI CTCTTC 3 cut(s) 303, 642, 1019
EclXI CGGCCG 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 1045
Eco47I GGWCC 1 cut(s) 815
Eco52I CGGCCG 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 210
Eco88I CYCGRG 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 815
EcoT22I ATGCAT 2 cut(s) 286, 382
EcoT38I GRGCYC 1 cut(s) 1045
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 9 cut(s) 217, 226, 380, 523, 588, 1097, 1147, 1180, 1194
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 2 cut(s) 828, 1139
FatI CATG 9 cut(s) 213, 222, 376, 519, 584, 1093, 1143, 1176, 1190
FauI CCCGC 3 cut(s) 406, 676, 1014
FblI GTMKAC 1 cut(s) 227
FokI GGATG 2 cut(s) 546, 740
FriOI GRGCYC 1 cut(s) 1045
FspBI CTAG 2 cut(s) 728, 990
GlaI GCGC 2 cut(s) 688, 997
GsaI CCCAGC 2 cut(s) 1160, 1167
GsuI CTGGAG 2 cut(s) 311, 475
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 4 cut(s) 460, 486, 1116, 1135
HapII CCGG 4 cut(s) 66, 111, 233, 642
HgaI GACGC 1 cut(s) 238
HhaI GCGC 2 cut(s) 689, 998
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 9 cut(s) 217, 226, 380, 523, 588, 1097, 1147, 1180, 1194
Hin6I GCGC 2 cut(s) 687, 996
HinP1I GCGC 2 cut(s) 687, 996
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HindIII AAGCTT 2 cut(s) 194, 921
HinfI GANTC 7 cut(s) 75, 163, 322, 699, 837, 955, 1107
HpaII CCGG 4 cut(s) 66, 111, 233, 642
HphI GGTGA 3 cut(s) 631, 656, 832
Hpy166II GTNNAC 4 cut(s) 116, 228, 717, 769
Hpy188I TCNGA 7 cut(s) 80, 171, 327, 806, 944, 968, 1023
Hpy188III TCNNGA 3 cut(s) 728, 757, 961
Hpy8I GTNNAC 4 cut(s) 116, 228, 717, 769
Hpy99I CGWCG 3 cut(s) 44, 232, 788
HpyAV CCTTC 2 cut(s) 1097, 1183
HpyCH4III ACNGT 2 cut(s) 344, 619
HpyCH4IV ACGT 2 cut(s) 209, 786
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 391, 896, 980, 1063
HpyF3I CTNAG 6 cut(s) 168, 200, 386, 398, 1046, 1209
HpySE526I ACGT 2 cut(s) 209, 786
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 9 cut(s) 217, 226, 380, 523, 588, 1097, 1147, 1180, 1194
HspAI GCGC 2 cut(s) 687, 996
Kzo9I GATC 2 cut(s) 963, 968
LguI GCTCTTC 2 cut(s) 303, 642
LmnI GCTCC 4 cut(s) 209, 404, 445, 586
Lsp1109I GCAGC 7 cut(s) 381, 859, 964, 967, 986, 1005, 1147
LweI GCATC 5 cut(s) 71, 271, 389, 886, 1066
MaeI CTAG 2 cut(s) 728, 990
MaeII ACGT 2 cut(s) 209, 786
MaeIII GTNAC 1 cut(s) 338
MalI GATC 2 cut(s) 965, 970
MbiI CCGCTC 1 cut(s) 402
MboI GATC 2 cut(s) 963, 968
MboII GAAGA 8 cut(s) 21, 24, 54, 320, 331, 634, 659, 1036
MhlI GDGCHC 1 cut(s) 1045
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 6 cut(s) 330, 798, 852, 861, 984, 1148
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 3 cut(s) 172, 831, 949
MmeI TCCRAC 2 cut(s) 352, 829
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 2 cut(s) 286, 382
MroXI GAANNNNTTC 1 cut(s) 923
MscI TGGCCA 1 cut(s) 486
MseI TTAA 6 cut(s) 261, 333, 632, 737, 749, 851
MslI CAYNNNNRTG 3 cut(s) 212, 492, 1098
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 977, 1160
MspI CCGG 4 cut(s) 66, 111, 233, 642
MspR9I CCNGG 3 cut(s) 67, 234, 642
Mva1269I GAATGC 1 cut(s) 1035
MvnI CGCG 1 cut(s) 427
MwoI GCNNNNNNNGC 5 cut(s) 210, 391, 896, 980, 1063
NciI CCSGG 3 cut(s) 67, 234, 642
NdeII GATC 2 cut(s) 963, 968
NlaIII CATG 9 cut(s) 217, 226, 380, 523, 588, 1097, 1147, 1180, 1194
NlaIV GGNNCC 1 cut(s) 816
NmeAIII GCCGAG 1 cut(s) 1161
NsiI ATGCAT 2 cut(s) 286, 382
NspI RCATGY 4 cut(s) 226, 380, 1097, 1194
OliI CACNNNNGTG 1 cut(s) 492
PaeI GCATGC 1 cut(s) 1194
PciI ACATGT 1 cut(s) 222
PciSI GCTCTTC 2 cut(s) 303, 642
PctI GAATGC 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 923
PfeI GAWTC 4 cut(s) 75, 322, 699, 1107
PflMI CCANNNNNTGG 1 cut(s) 1163
PfoI TCCNGGA 1 cut(s) 65
PleI GAGTC 3 cut(s) 171, 831, 949
PmaCI CACGTG 1 cut(s) 210
PmlI CACGTG 1 cut(s) 210
PpsI GAGTC 3 cut(s) 171, 831, 949
Ppu21I YACGTR 1 cut(s) 210
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 222
PshBI ATTAAT 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 815
PspCI CACGTG 1 cut(s) 210
PspFI CCCAGC 2 cut(s) 1156, 1163
PspN4I GGNNCC 1 cut(s) 816
PspPI GGNCC 2 cut(s) 815, 1133
PspPPI RGGWCCY 1 cut(s) 815
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 4 cut(s) 689, 977, 980, 1163
PvuII CAGCTG 2 cut(s) 977, 1160
RsaI GTAC 6 cut(s) 25, 556, 718, 770, 1061, 1121
RsaNI GTAC 6 cut(s) 24, 555, 717, 769, 1060, 1120
RseI CAYNNNNRTG 3 cut(s) 212, 492, 1098
SalI GTCGAC 1 cut(s) 226
SapI GCTCTTC 2 cut(s) 303, 642
SaqAI TTAA 6 cut(s) 261, 333, 632, 737, 749, 851
Sau3AI GATC 2 cut(s) 963, 968
Sau96I GGNCC 2 cut(s) 815, 1133
SbfI CCTGCAGG 1 cut(s) 823
SchI GAGTC 3 cut(s) 172, 831, 949
ScrFI CCNGG 3 cut(s) 67, 234, 642
SdaI CCTGCAGG 1 cut(s) 823
SduI GDGCHC 1 cut(s) 1045
SfaNI GCATC 5 cut(s) 71, 271, 389, 886, 1066
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 3 cut(s) 212, 492, 1098
SmlI CTYRAG 2 cut(s) 501, 1010
SmoI CTYRAG 2 cut(s) 501, 1010
SphI GCATGC 1 cut(s) 1194
Sse8387I CCTGCAGG 1 cut(s) 823
Sse9I AATT 6 cut(s) 330, 798, 852, 861, 984, 1148
SsiI CCGC 9 cut(s) 391, 402, 413, 427, 434, 461, 669, 876, 1007
SspMI CTAG 2 cut(s) 728, 990
StyD4I CCNGG 3 cut(s) 65, 232, 640
TaaI ACNGT 2 cut(s) 344, 619
TaiI ACGT 2 cut(s) 212, 789
TaqI TCGA 3 cut(s) 227, 637, 783
TasI AATT 6 cut(s) 330, 798, 852, 861, 984, 1148
TatI WGTACW 4 cut(s) 716, 768, 1059, 1119
TauI GCSGC 3 cut(s) 394, 463, 878
TfiI GAWTC 4 cut(s) 75, 322, 699, 1107
Tru1I TTAA 6 cut(s) 261, 333, 632, 737, 749, 851
Tru9I TTAA 6 cut(s) 261, 333, 632, 737, 749, 851
TscAI CASTG 4 cut(s) 414, 487, 499, 1054
TseI GCWGC 7 cut(s) 394, 872, 974, 977, 980, 993, 1160
TspDTI ATGAA 5 cut(s) 500, 663, 1160, 1165, 1200
TspRI CASTG 4 cut(s) 414, 487, 499, 1054
Van91I CCANNNNNTGG 1 cut(s) 1163
VpaK11BI GGWCC 1 cut(s) 815
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 1 cut(s) 1148
XbaI TCTAGA 1 cut(s) 727
XceI RCATGY 4 cut(s) 226, 380, 1097, 1194
XmiI GTMKAC 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 923
XspI CTAG 2 cut(s) 728, 990
Zsp2I ATGCAT 2 cut(s) 286, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.