Rw2G003270

Adenine nucleotide transporter BT1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014630
Physical Location & Seq
Reverse (-)
3068773 .. 3070239
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003270.1

Sequence Viewer

Length: 1239 bp
ATGGGTAGAAGAAGATTGCAAGTGATTGAACATGCGAGTAAAGACTTTGTGTTGTTCTCTTCTGGGATCAAGATTCAATGGAGTTTTCTAGAGAACGCTTCTCGTGGCTTATTTGCAAGTATTGGTCAAGTGGCAATGGGAATGGGATTTGGTATTTCACCAAATCCTCAGAATCTTAGGATGGACAGCACCACAAAGCTTCTGAGTTCCACACACATGAGATATGTGCCTATGCCGATTGTGAGCATTGGTGGAGTTGAAGAGTTGGTGATGGAAGAAGTACTAGAGATTGAAGAAGAAGCAGATGTTGTGAGCATGAACAAGATGAATAACAGAAGAGATGGTTTTGGAATGAAAATCAAGATTGCTAATTCATCACTAAGAAGGTTGCTGAGTGGAGCAATAGCAGGTGTTGTGTCAAGGACAGCAGTTGCACCATTGGAGACCATAAGGACCAACTTAATGGTGGGGAGCTGTGGCAACTCATCCAGTGCAGTATTTCAATCCATCATGGGAGCTGATGGATGGCAAGGCTTGTATAGGGGCAATTTGGTCAATGTCATTCGCGTTGCCCCAAGCAAGGGTATTGAGTTATTTGTCTTTGATATGGTGAACAAGTACTTGAGCAGACCTGGGGAAGAGCTGAAAATCCCTATTCCTGCTTCATCAATCGCAGGCGCCCTTGCTGGATTTAGCTCAACCTTGTGTACATACCCTCTCGAGCTGTTAAAAACACGATTAACTGTTCAGAGAGGAGTGTACAAGAACTTTTTCGATGCATTTTCCAAAATTGTTAAAGAGGGAGGACCTGCAGAGCTATATAGAGGCCTTACACCAAGCCTAATAGGAGTCATCCCATATGCTGCCACCAACTATTTCGCTTATGATACACTTTCAAAAGCTTACAAGAAGGCTTTTAAAAAGGAGGAAATCGGAAATGTAACGACTTTGTTGATTGGCTCAGCAGCTGCTGCATTTTCAAGCAGTGCAACTTTCCCACTTGAGGTGGCTCGGAAGCAAATGCAGGTCAATGGGAGACAATACAAGCACATATTTCATGCTCTTTGTAGCATTTTTGAAAGTGAAGGAGTCGCTGGTTTGTACAGAGGGTTAGGGCCAAGCTACATGAAATTGGTCCCTGCTGCTGGGATATCCTTCATGTGCTACGAAGCATGCAAGAGGATACTCACTGAGAACAAGAAGATACTCACTGAGAACGGGAATCAGGAAGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

412

Amino Acids

45.02

Weight (kDa)

9.32

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 125 - 210 4.9e-19 Mitochondrial carrier protein
Mito_carr PF00153 218 - 304 3.7e-24 Mitochondrial carrier protein
Mito_carr PF00153 313 - 399 4.3e-23 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014630)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03510
malus_domestica MD02G1034100.v1.1 MD15G1174900.v1.1
prunus_persica Prupe.7G241000_v2.0.a1
pyrus_communis pycom02g02790 pycom15g15640
rosa_chinensis RchiOBHm_Chr2g0088721
rosa_laevigata RLG00000015979
rosa_multiflora Rmu_sc0006835.1_g000004
rosa_roxburghii Rroxscaffold_2G00152370
rosa_rugosa Rorug01G0486500 Rorug01G0486600
rosa_samantha Rh2AG041700 Rh2BG040400 Rh2CG041200 Rh2DG040800
rosa_wichuraiana Rw2G003270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc36I ACCTGC 3 cut(s) 398, 817, 1015
AccB1I GGYRCC 1 cut(s) 677
AccII CGCG 1 cut(s) 567
AclWI GGATC 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 678
AfaI GTAC 5 cut(s) 282, 620, 709, 761, 1103
AfiI CCNNNNNNNGG 4 cut(s) 580, 581, 1003, 1145
AgsI TTSAA 8 cut(s) 29, 77, 260, 293, 503, 897, 981, 1079
AjnI CCWGG 1 cut(s) 631
Alw26I GTCTC 2 cut(s) 437, 1030
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 2 cut(s) 968, 971
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 2 cut(s) 826, 1115
ApeKI GCWGC 5 cut(s) 863, 965, 968, 971, 1142
Asp700I GAANNNNTTC 1 cut(s) 770
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 4 cut(s) 453, 806, 1115, 1135
AsuHPI GGTGA 3 cut(s) 150, 280, 622
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 3 cut(s) 453, 806, 1135
BanI GGYRCC 1 cut(s) 677
BauI CACGAG 1 cut(s) 102
BbvI GCAGC 5 cut(s) 850, 955, 958, 977, 1129
BccI CCATC 6 cut(s) 175, 265, 335, 515, 515, 519
BciT130I CCWGG 1 cut(s) 633
BciVI GTATCC 1 cut(s) 1176
BcoDI GTCTC 2 cut(s) 437, 1030
BfaI CTAG 2 cut(s) 89, 284
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 681
BfuAI ACCTGC 3 cut(s) 398, 817, 1015
BfuI GTATCC 1 cut(s) 1176
BisI GCNGC 5 cut(s) 864, 966, 969, 972, 1143
BlpI GCTNAGC 1 cut(s) 961
BlsI GCNGC 5 cut(s) 865, 967, 970, 973, 1144
BmcAI AGTACT 2 cut(s) 282, 620
Bme1390I CCNGG 1 cut(s) 633
Bme18I GGWCC 3 cut(s) 453, 806, 1135
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 4 cut(s) 453, 806, 1115, 1135
BmiI GGNNCC 2 cut(s) 679, 1137
BmrFI CCNGG 1 cut(s) 633
BmsI GCATC 1 cut(s) 766
Bpu1102I GCTNAGC 1 cut(s) 961
BpuEI CTTGAG 2 cut(s) 643, 1022
BsaHI GRCGYC 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 632
Bsc4I CCNNNNNNNGG 4 cut(s) 580, 581, 1003, 1145
Bse1I ACTGG 1 cut(s) 489
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 632
BseGI GGATG 4 cut(s) 186, 485, 530, 852
BseLI CCNNNNNNNGG 4 cut(s) 580, 581, 1003, 1145
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 6 cut(s) 182, 194, 383, 975, 1182, 1203
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 5 cut(s) 850, 955, 958, 977, 1129
BseYI CCCAGC 1 cut(s) 1145
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 2 cut(s) 828, 1117
BshNI GGYRCC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 1121
BslI CCNNNNNNNGG 4 cut(s) 580, 581, 1003, 1145
BsmAI GTCTC 2 cut(s) 437, 1030
BsmFI GGGAC 1 cut(s) 1121
BsnI GGCC 2 cut(s) 828, 1117
Bso31I GGTCTC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 719
Bsp1407I TGTACA 3 cut(s) 707, 759, 1101
Bsp143I GATC 1 cut(s) 66
Bsp1720I GCTNAGC 1 cut(s) 961
BspANI GGCC 2 cut(s) 828, 1117
BspCNI CTCAG 6 cut(s) 181, 195, 384, 974, 1183, 1204
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 2 cut(s) 679, 1137
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 3 cut(s) 398, 817, 1015
BspPI GGATC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 141
BsrGI TGTACA 3 cut(s) 707, 759, 1101
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 1 cut(s) 632
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 1 cut(s) 678
BssSI CACGAG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 1 cut(s) 745
Bst6I CTCTTC 4 cut(s) 64, 255, 331, 633
BstACI GRCGYC 1 cut(s) 678
BstAPI GCANNNNNTGC 1 cut(s) 971
BstAUI TGTACA 3 cut(s) 707, 759, 1101
BstC8I GCNNGC 2 cut(s) 676, 1174
BstDEI CTNAG 8 cut(s) 168, 176, 203, 380, 392, 961, 1191, 1212
BstF5I GGATG 4 cut(s) 186, 485, 530, 852
BstFNI CGCG 1 cut(s) 567
BstH2I RGCGCY 1 cut(s) 681
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 2 cut(s) 437, 1030
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 971
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 2 cut(s) 35, 1176
BstSCI CCNGG 1 cut(s) 631
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 5 cut(s) 850, 955, 958, 977, 1129
BstXI CCANNNNNNTGG 1 cut(s) 463
BsuI GTATCC 1 cut(s) 1176
BsuRI GGCC 2 cut(s) 828, 1117
BtsCI GGATG 4 cut(s) 186, 485, 530, 852
BtsI GCAGTG 1 cut(s) 991
BtsIMutI CAGTG 4 cut(s) 496, 991, 1188, 1209
BveI ACCTGC 3 cut(s) 398, 817, 1015
Cac8I GCNNGC 2 cut(s) 676, 1174
CaiI CAGNNNCTG 2 cut(s) 968, 971
CfoI GCGC 1 cut(s) 680
Cfr13I GGNCC 4 cut(s) 453, 806, 1115, 1135
Csp6I GTAC 5 cut(s) 281, 619, 708, 760, 1102
CviAII CATG 8 cut(s) 32, 217, 316, 511, 1058, 1126, 1159, 1173
CviQI GTAC 5 cut(s) 281, 619, 708, 760, 1102
DdeI CTNAG 8 cut(s) 168, 176, 203, 380, 392, 961, 1191, 1212
DinI GGCGCC 1 cut(s) 679
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
DraI TTTAAA 1 cut(s) 919
Eam1104I CTCTTC 4 cut(s) 64, 255, 331, 633
EarI CTCTTC 4 cut(s) 64, 255, 331, 633
Eco147I AGGCCT 1 cut(s) 828
Eco31I GGTCTC 1 cut(s) 437
Eco32I GATATC 1 cut(s) 1152
Eco47I GGWCC 3 cut(s) 453, 806, 1135
Eco88I CYCGRG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 631
EcoRV GATATC 1 cut(s) 1152
EcoT22I ATGCAT 1 cut(s) 781
EgeI GGCGCC 1 cut(s) 679
EheI GGCGCC 1 cut(s) 679
FaeI CATG 8 cut(s) 35, 220, 319, 514, 1061, 1129, 1162, 1176
FaqI GGGAC 1 cut(s) 1121
FatI CATG 8 cut(s) 31, 216, 315, 510, 1057, 1125, 1158, 1172
FauNDI CATATG 1 cut(s) 859
Fnu4HI GCNGC 5 cut(s) 864, 966, 969, 972, 1143
FokI GGATG 4 cut(s) 193, 472, 537, 839
Fsp4HI GCNGC 5 cut(s) 864, 966, 969, 972, 1143
FspBI CTAG 2 cut(s) 89, 284
GlaI GCGC 1 cut(s) 679
GluI GCNGC 5 cut(s) 864, 966, 969, 972, 1143
GsaI CCCAGC 1 cut(s) 1149
HaeII RGCGCY 1 cut(s) 681
HaeIII GGCC 2 cut(s) 828, 1117
HhaI GCGC 1 cut(s) 680
Hin1I GRCGYC 1 cut(s) 678
Hin1II CATG 8 cut(s) 35, 220, 319, 514, 1061, 1129, 1162, 1176
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HindIII AAGCTT 2 cut(s) 197, 900
HinfI GANTC 5 cut(s) 73, 172, 849, 1089, 1222
HphI GGTGA 3 cut(s) 150, 280, 622
Hpy166II GTNNAC 3 cut(s) 613, 708, 760
Hpy188I TCNGA 5 cut(s) 171, 204, 750, 935, 1014
Hpy188III TCNNGA 5 cut(s) 70, 89, 361, 719, 1226
Hpy8I GTNNAC 3 cut(s) 613, 708, 760
HpyAV CCTTC 4 cut(s) 378, 904, 1079, 1165
HpyCH4III ACNGT 1 cut(s) 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 971
HpyF3I CTNAG 8 cut(s) 168, 176, 203, 380, 392, 961, 1191, 1212
Hsp92I GRCGYC 1 cut(s) 678
Hsp92II CATG 8 cut(s) 35, 220, 319, 514, 1061, 1129, 1162, 1176
HspAI GCGC 1 cut(s) 678
KasI GGCGCC 1 cut(s) 677
Kzo9I GATC 1 cut(s) 66
LguI GCTCTTC 1 cut(s) 633
LmnI GCTCC 3 cut(s) 398, 471, 515
Lsp1109I GCAGC 5 cut(s) 850, 955, 958, 977, 1129
LweI GCATC 1 cut(s) 766
MaeI CTAG 2 cut(s) 89, 284
MaeIII GTNAC 1 cut(s) 940
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MluCI AATT 4 cut(s) 370, 547, 789, 1130
Mly113I GGCGCC 1 cut(s) 678
MlyI GAGTC 2 cut(s) 858, 1098
Mph1103I ATGCAT 1 cut(s) 781
MroXI GAANNNNTTC 1 cut(s) 770
MseI TTAA 5 cut(s) 461, 728, 740, 795, 918
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 968
MspR9I CCNGG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 1 cut(s) 971
NarI GGCGCC 1 cut(s) 678
NdeI CATATG 1 cut(s) 859
NdeII GATC 1 cut(s) 66
NlaIII CATG 8 cut(s) 35, 220, 319, 514, 1061, 1129, 1162, 1176
NlaIV GGNNCC 2 cut(s) 679, 1137
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 2 cut(s) 35, 1176
PaeI GCATGC 1 cut(s) 1176
PaeR7I CTCGAG 1 cut(s) 719
PaqCI CACCTGC 1 cut(s) 398
PceI AGGCCT 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 770
PfeI GAWTC 3 cut(s) 73, 172, 1222
PkrI GCNGC 5 cut(s) 865, 967, 970, 973, 1144
PleI GAGTC 2 cut(s) 857, 1097
PluTI GGCGCC 1 cut(s) 681
PpsI GAGTC 2 cut(s) 857, 1097
PpuMI RGGWCCY 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 1145
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 2 cut(s) 679, 1137
PspPI GGNCC 4 cut(s) 453, 806, 1115, 1135
PspPPI RGGWCCY 1 cut(s) 806
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 2 cut(s) 968, 971
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 5 cut(s) 282, 620, 709, 761, 1103
RsaNI GTAC 5 cut(s) 281, 619, 708, 760, 1102
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 5 cut(s) 461, 728, 740, 795, 918
SatI GCNGC 5 cut(s) 864, 966, 969, 972, 1143
Sau3AI GATC 1 cut(s) 66
Sau96I GGNCC 4 cut(s) 453, 806, 1115, 1135
ScaI AGTACT 2 cut(s) 282, 620
SchI GAGTC 2 cut(s) 858, 1098
ScrFI CCNGG 1 cut(s) 633
SfaNI GCATC 1 cut(s) 766
SfcI CTRYAG 1 cut(s) 810
SfoI GGCGCC 1 cut(s) 679
Sfr274I CTCGAG 1 cut(s) 719
SinI GGWCC 3 cut(s) 453, 806, 1135
SlaI CTCGAG 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 3 cut(s) 622, 719, 1001
SmoI CTYRAG 3 cut(s) 622, 719, 1001
SphI GCATGC 1 cut(s) 1176
Sse9I AATT 4 cut(s) 370, 547, 789, 1130
SseBI AGGCCT 1 cut(s) 828
SspDI GGCGCC 1 cut(s) 677
SspMI CTAG 2 cut(s) 89, 284
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 1 cut(s) 631
TaaI ACNGT 1 cut(s) 745
TaqI TCGA 2 cut(s) 720, 774
TasI AATT 4 cut(s) 370, 547, 789, 1130
TatI WGTACW 5 cut(s) 280, 618, 707, 759, 1101
TfiI GAWTC 3 cut(s) 73, 172, 1222
Tru1I TTAA 5 cut(s) 461, 728, 740, 795, 918
Tru9I TTAA 5 cut(s) 461, 728, 740, 795, 918
TscAI CASTG 4 cut(s) 496, 991, 1195, 1216
TseI GCWGC 5 cut(s) 863, 965, 968, 971, 1142
TspDTI ATGAA 8 cut(s) 332, 341, 363, 368, 654, 1046, 1142, 1147
TspRI CASTG 4 cut(s) 496, 991, 1195, 1216
VpaK11BI GGWCC 3 cut(s) 453, 806, 1135
XbaI TCTAGA 1 cut(s) 88
XceI RCATGY 2 cut(s) 35, 1176
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 719
XmnI GAANNNNTTC 1 cut(s) 770
XspI CTAG 2 cut(s) 89, 284
ZrmI AGTACT 2 cut(s) 282, 620
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.