MD02G1049000.v1.1

Aldehyde dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018897
Physical Location & Seq
Forward (+)
3849798 .. 3850322
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1049000.v1.1.491

Sequence Viewer

Length: 258 bp
ATGGCTAGTGATTCCAACGACAGCTTAGCTTCCGAGTCTTCCGTCAAGATTCCGACAATAAAGTACACCAAGCTCTTCATCAATGGAGAATTCGTTGATTCCGTTTCAGGGAAAACATTTGAGACGATGGATCCAAGAACAGGGGAGGTGAAAGCTAGAGTTGCAGAAGGAGATAAGGAACATGTTGATTTGGCTGTCGAGGCTGCACGCGCTGCCTTCGAAAATGGCCCTTGGCCTCGCTTGCCCGGCAGTGTATAG

Protein Analysis

86

Amino Acids

9.16

Weight (kDa)

4.86

Isoelectric Point (pI)

8.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 31 - 82 4.9e-07 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018897)

Species Orthologous Gene IDs
malus_domestica MD02G1049000.v1.1
pyrus_communis pycom02g04110 pycom02g04140
rosa_chinensis RchiOBHm_Chr3g0491961
rosa_multiflora Rmu_co8011570.1_g000001 Rmu_sc0004195.1_g000001
rosa_samantha Rh2AG056100 Rh2BG054700 Rh2CG057000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 210
AclWI GGATC 2 cut(s) 125, 138
AcsI RAATTY 1 cut(s) 89
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 2 cut(s) 108, 140
AflIII ACRYGT 1 cut(s) 181
AluBI AGCT 4 cut(s) 24, 29, 73, 155
AluI AGCT 4 cut(s) 24, 29, 73, 155
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 2 cut(s) 125, 138
AoxI GGCC 2 cut(s) 226, 233
ApeKI GCWGC 2 cut(s) 203, 212
ApoI RAATTY 1 cut(s) 89
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 219
BamHI GGATCC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 190, 199
BccI CCATC 1 cut(s) 121
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 6, 156
BisI GCNGC 2 cut(s) 204, 213
BlpI GCTNAGC 1 cut(s) 25
BlsI GCNGC 2 cut(s) 205, 214
Bme1390I CCNGG 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 140
BseDI CCNNGG 1 cut(s) 230
BseLI CCNNNNNNNGG 2 cut(s) 108, 140
BseXI GCAGC 2 cut(s) 190, 199
BsgI GTGCAG 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 228, 235
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 108, 140
BsmAI GTCTC 1 cut(s) 116
BsmBI CGTCTC 1 cut(s) 116
BsnI GGCC 2 cut(s) 228, 235
Bsp119I TTCGAA 1 cut(s) 219
Bsp143I GATC 1 cut(s) 130
Bsp1720I GCTNAGC 1 cut(s) 25
BspANI GGCC 2 cut(s) 228, 235
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 132
BspPI GGATC 2 cut(s) 125, 138
BspQI GCTCTTC 1 cut(s) 80
BspT104I TTCGAA 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 208, 242
BstDEI CTNAG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 6 cut(s) 161, 200, 209, 212, 241, 246
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 244
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 190, 199
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 2 cut(s) 228, 235
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 208, 242
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 64
CviAII CATG 1 cut(s) 182
CviJI RGCY 9 cut(s) 5, 24, 29, 73, 155, 194, 203, 228, 235
CviKI_1 RGCY 9 cut(s) 5, 24, 29, 73, 155, 194, 203, 228, 235
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 1 cut(s) 25
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 1 cut(s) 185
FaiI YATR 2 cut(s) 183, 256
FatI CATG 1 cut(s) 181
Fnu4HI GCNGC 2 cut(s) 204, 213
Fsp4HI GCNGC 2 cut(s) 204, 213
FspBI CTAG 2 cut(s) 6, 156
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 204, 213
HaeIII GGCC 2 cut(s) 228, 235
HapII CCGG 1 cut(s) 246
HhaI GCGC 1 cut(s) 212
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 4 cut(s) 11, 35, 49, 98
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 2 cut(s) 34, 54
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 1 cut(s) 66
HpyAV CCTTC 2 cut(s) 161, 226
HpyCH4V TGCA 2 cut(s) 164, 206
HpyF10VI GCNNNNNNNGC 6 cut(s) 161, 200, 209, 212, 241, 246
HpyF3I CTNAG 1 cut(s) 25
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 80
LpnPI CCDG 2 cut(s) 93, 126
Lsp1109I GCAGC 2 cut(s) 190, 199
MaeI CTAG 2 cut(s) 6, 156
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 2 cut(s) 30, 67
MflI RGATCY 1 cut(s) 130
MluCI AATT 1 cut(s) 89
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 39, 77
MnlI CCTC 3 cut(s) 139, 193, 246
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 6 cut(s) 161, 200, 209, 212, 241, 246
NciI CCSGG 1 cut(s) 246
NdeII GATC 1 cut(s) 130
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 1 cut(s) 132
NspI RCATGY 1 cut(s) 185
NspV TTCGAA 1 cut(s) 219
PciI ACATGT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 3 cut(s) 11, 49, 98
PkrI GCNGC 2 cut(s) 205, 214
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PscI ACATGT 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 80
SatI GCNGC 2 cut(s) 204, 213
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 246
SetI ASST 5 cut(s) 26, 31, 75, 150, 157
SfuI TTCGAA 1 cut(s) 219
Sse9I AATT 1 cut(s) 89
SspMI CTAG 2 cut(s) 6, 156
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 230
TaqI TCGA 2 cut(s) 198, 219
TasI AATT 1 cut(s) 89
TatI WGTACW 1 cut(s) 63
TfiI GAWTC 3 cut(s) 11, 49, 98
TscAI CASTG 1 cut(s) 256
TseI GCWGC 2 cut(s) 203, 212
TspDTI ATGAA 1 cut(s) 67
TspGWI ACGGA 2 cut(s) 31, 91
TspRI CASTG 1 cut(s) 256
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 185
XspI CTAG 2 cut(s) 6, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.