MD02G1062500.v1.1

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011282
Physical Location & Seq
Forward (+)
5011943 .. 5018557
6615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1062500.v1.1.491

Sequence Viewer

Length: 3867 bp
ATGTTTGCACTTTTCACTGTATATATTCAGGAAGAAGGAACCAAAAGAATTCCCCTCTTCCTCCCTCTCTTTCTCTCTCTTTCCTGCATCTCCCAAATCATTGGAGCCTCGAAATCTTTCTTGATTCTTGCTCCATTCAACAAAGATTCAGATTCTAAGACAACCCTTTTGAAGATTTTAACCAAAAGAAATCGGAAAATGTCACAAGAAAGAGTGCATCTCTTGACATTAGTACTAATCCTTCTTGTTTTCATGACGATGGTCGAGAAAGGTGCGAGCTTTGGAGTGAACTGGGGGACCATGGCGACCCACCAGCTCCCGCCGGAGAAGGTAGTCCGGATTCTGGAGGACAACGGATTCGACAAGGTGAAATTGTTCGAAGCGGACGAGAGAATATTGGCCGCTCTCACTGGGACTAAAATTGAGGTGATGCTGGCCATACCCAATGTCATGTTGCAGGAGATGAGCCAGGATCCTGTGGCTGCAGCTACTTGGGTGGATGCCAATGTCACAAGTTACTGCTACTCTGGTGGAGTTGTTATCAAGTATGTAGCTGTAGGCAATGAGCCCTTTCTGAAAACGTACAATGGCACCTATCTACAAACCACATTACCAGCTCTCAAGAACATCCAGGAAGCCCTAAACAATGCAGGACTTGGGTCACAAGTCAAAGCCACCGTCCCCTTCAACGCCGACGTCTACTTCTCCCCTGACTCAGACCCGGTTCCATCTACTGGAGACTTCCGCCCTGAAGTTCGAGACTCCATAATCAAGATCATCCAGTACTTGTACACCAATGAGGCACCATTCACAGTTAACATTTACCCTTTTCTCAGCCTCTATGGCAATTCCTATTTTCCTTTCGGGTTTGCATTTTTCGATGGAAAAAGCAAGCCGATTAAAGATGGTGATCTGCTCTACACCAATGTGTTTGATGCAAACTTTGACACCCTAGTTTGGTCTCTGACCAAAGCCGGCTTCCCTGAAATGAAGATCATAGTTGGAGAGGTGGGCTGGCCAACTGATGGTGACATAAACGCGAATATCCGCAACGCGAAGAGGTTCAATCAGGGTATGATCCAGCATGCCATGAGTGGCAATGGAACCCCAGCAAGGACAGGCAAACTCGATGGCTACCTATTCAGCCTTATTGACGAAAACATGAAAAGCATTGCTCCCGGAAACTTTGAGAGGCACTGGGGGATATTCGAGTATGATGGGAAGCCGAAATATGATCTGGATTTGTCAGGCGCTATGCGAAACAAGGGCCTTGCAGCTGCAGAAGATGTGGAGTATATGCGGAGAAGTTGGTGTGTTTTGGACCCAGATGTTAAGTCTTTGGATGATTTGGCAAAAAGCATTGACTATGCTTGCACACTCTCAGATTGCACTTCATTGGGATATGGAAGTTCTTGCAACAATCTCACTCTCCAAGGGAATGCTTCGTATGCATTCAACATGTATTACCAGGTGAACAGCCAGAAGAGCTGGACCTGCGATTTTTCGGGTTTGGCTGTGGTGACCGATGAGGATCCTTCCGTTGGGGACTGCGAGTTTCCGGTGATGATTTCGTATGCTGCTCCATCACTGTTGCACAGCAGGGGACTTTTGCATTTTGTGATGAAAATTGTGGGAGGATTTCTGTTAGTACTCCTCCACAGATTCAAACCTCCACCTTCTCTCTCCGTCTCTCTCTCTCTCTCTCTCTCTCTTCTCACTCACACACACACAACAATGGCGTCGCTCACTCCAGGAATCCTCCTGAAGCTTCTCCAGTCAATGAACTCCTCTACCAAAGTCACCGGCGACCACCGCTCCGCCCTCCTCCAAGTCATCGGCATAGTCCCTGCTCTCGCCGGCTCCGACCTCTGGCCCAACCAGGGCTTCTACGTCCAGCTATCCGACTCCCTCAACTCCACTTATGTCTCCCTTTCTGATCGTGACACCGACCTCATCCTCACCAACCGCCTCCAGCTGGGCCAGTTCGCCTACGTCGACCGCTTCGATTTCGACTCCCCAGTCCCCCGCGTCGTCGGAATCCGTCCCATCGCCGGTCGACATCCCTTCCTCGGCACCCCGGAGCCCCTTGTCGCCCGTATCTCGGCCTCGAAACGAGAGTTTGTGATCCAGCCTGTGTCGGATTCCGACCAGTCCACCGATTTCATGGCGATTTACCTGTCCAATAAGAAGCAGGAGCAGGTTGTGAGGAATGACAACAAAGAAGCCAAGGTTGAGAAGGCACGACCATCAAGGCAGCCTCTTGCTCCTCGAGACAATGTCAATTCGGGTGGGAATGTGAATTCGAATTCAAGTACAGATGAGGCTAAGAAGATCCCCGATCGGCCGGCTTCCAGGTTTTCATCTCCGGCCGGTGCAAAGAGGCCCGTGTCAGTCGGAAAGAAGAACCCGGCGTCGGCTGAGAGAGATCCATCCCCCGCAGGAAAAGGAAAGAGATCGGGATCTCCAGCCCCGTCGAAATGCGTTGTTCCGAGCTTAATTGTGGCGAAGGAAGAGAACCGGAAGGTGTCGAAAGAGGCGGCGATTATAGTGCCGTCGAGGTACCGACAGCCATCCCCAATTGGGCAGCGGAGGCAGCCTTCACCAAATTCCCGGAGGGCTTCGCTTTCTCCCGGGAGGCGGTTGTCTGGAGGAGTGAAGGACTCTGCTGCCAAGAAGAAGACGATAAGTATCGTTGCAGGGATTTCGAAGGTTTCAGAAGCCCTCATTGGGTCTGGAAAGAGTAATCGGAAGGGCTGGGATGAGTCGCCGATGGTGGAGGAGAAAGAGAAGCCTTCGGCCAAGATCAAACCAGATTTGCAAGCATTTATAAGGACTCAGGCTGCTCTTGCGCGACGATTAAGCGATGCGAAAGGTGGAAGTCCGAATGGCGGTGATGATTCTTCCGGCAATGAGAAAACAAAATCTGGGTCACCGGCAGATTTTGTGGTCCAAGAGAAACCCAGCTGCGCAGCAGCTCCGAGCATCACCGTCCATGAAAAAAAATGGACTGATGGAAGTGTCTCACTTGATGCAGTCTCTTCAGACCTCGCAAGGCTTGGAAAGGAAGCTTTGCAGAGGAAAGTTCTTGCTTCTGCAGCTGCAGCTGAAGCACTCGAGGAGGCCATTGCCACTGAGTCTCTTGTGAGGAAATTAAGCATGTTTGCGGAACTCTCTTCCACATCCAAGGTCGGGAACCCTCTACCCGCCATTGACCGCTTCTTTTCGATCTATGATGAAGTTGTTAAATCAACCACACTTGTTGAATCAATTGCTGGTAATCGCAATTCGGATACATCTAACAATGCTCAAATTCCCACTGAGCAATCGAAACCCGTTTCACTTTGGGTTGAAGCTGCTTTAGCTACTGATCTCGGCATTGTCTCTCTCCTTACCACCCAAGACAATGAAACTCCATCAGCTCTGCAGAAAAGTCTGTCAAAACGACAATCCCTCAACACACCTCCCAAATCCCATCTCAAGATTTCCCCTTCCTCTTCACCGCAGTCCAATGCTCGCGTTGGGACGTGGACAAAGGGTCACGGAATGAAAGACACAGTCGAGCTTGCCACTCATCTGCAATCCGAGATGCAAATGTGGTTTCTGCAGTTTGTTGAGAAGGCCCTTGATGCCGGTTTTCGTGTGTTTGGAGAGTGCGCAGCTGATGGTGGCAAACTGCCAATCGACAGTGGCTCCATTGCAGCTGTTCTTTCGCAGTTGAAGCGGGTGAATGAGTGGCTGGATCGAGTCGTCTCGAAAAGGAATGAACTGAATGCGAAGGTTGATCGGCTGAAGAGGAAGATCTACGGCTTTGTTATTCAACATGTTGGGACGACGGTCGACAGCTCCACCCCTCTTGCTTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

1289

Amino Acids

140.26

Weight (kDa)

8.99

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 95 - 414 1.3e-59 Glycosyl hydrolases family 17
X8 PF07983 436 - 505 2.4e-16 X8 domain
DUF936 PF06075 582 - 698 7.7e-52 Plant protein of unknown function (DUF936)
DUF6857 PF21647 996 - 1281 5.7e-97 Domain of unknown function (DUF6857)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13370
fragaria_vesca FvH4_1g05440 FvH4_1g05440
malus_domestica MD02G1062500.v1.1 MD15G1190300.v1.1 MD17G1097200.v1.1
prunus_persica Prupe.7G226800_v2.0.a1
pyrus_communis pycom02g04340 pycom15g17050
rosa_chinensis RchiOBHm_Chr2g0091101
rosa_laevigata RLG00000016180
rosa_multiflora Rmu_sc0004851.1_g000007 Rmu_sc0005171.1_g000002
rosa_roxburghii Rroxscaffold_2G00150540
rosa_rugosa Rorug02G0013000
rosa_samantha Rh2AG059200 Rh2BG057600 Rh2CG059500 Rh2DG058600
rosa_wichuraiana Rw2G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2828
AasI GACNNNNNNGTC 1 cut(s) 2018
AatII GACGTC 1 cut(s) 699
Acc16I TGCGCA 2 cut(s) 2968, 3655
Acc36I ACCTGC 2 cut(s) 1502, 2188
Acc65I GGTACC 1 cut(s) 2559
AccB1I GGYRCC 4 cut(s) 590, 802, 2072, 2559
AccB7I CCANNNNNTGG 2 cut(s) 734, 1025
AccBSI CCGCTC 2 cut(s) 404, 1815
AccI GTMKAC 4 cut(s) 699, 1995, 2056, 3837
AccII CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
AccIII TCCGGA 1 cut(s) 336
AcoI YGGCCR 6 cut(s) 399, 435, 1016, 2342, 2367, 2796
AcsI RAATTY 5 cut(s) 48, 2299, 2305, 2605, 3309
AcuI CTGAAG 5 cut(s) 771, 1784, 3024, 3126, 3809
AcyI GRCGYC 3 cut(s) 696, 1739, 2411
AfaI GTAC 7 cut(s) 234, 584, 785, 791, 1650, 2314, 2561
AflIII ACRYGT 2 cut(s) 1458, 3820
AhdI GACNNNNNGTC 1 cut(s) 3534
AjiI CACGTC 1 cut(s) 3525
AjnI CCWGG 6 cut(s) 468, 630, 1467, 1750, 1878, 2351
AjuI GAANNNNNNNTTGG 6 cut(s) 962, 994, 1868, 1900, 2709, 2741
AleI CACNNNNGTG 1 cut(s) 926
Ama87I CYCGRG 3 cut(s) 2268, 2630, 3113
Aor13HI TCCGGA 1 cut(s) 336
ApoI RAATTY 5 cut(s) 48, 2299, 2305, 2605, 3309
ArsI GACNNNNNNTTYG 8 cut(s) 151, 183, 341, 373, 663, 695, 2912, 2944
Asp700I GAANNNNTTC 3 cut(s) 116, 374, 1061
Asp718I GGTACC 1 cut(s) 2559
AspLEI GCGC 4 cut(s) 1253, 2851, 2969, 3656
AspS9I GGNCC 9 cut(s) 297, 1267, 1321, 1491, 1872, 1978, 2382, 2947, 3619
AsuC2I CCSGG 7 cut(s) 722, 1179, 2078, 2408, 2611, 2631, 2632
AsuII TTCGAA 3 cut(s) 378, 2303, 2705
AvaI CYCGRG 3 cut(s) 2268, 2630, 3113
AvaII GGWCC 4 cut(s) 297, 1321, 1491, 2947
BalI TGGCCA 2 cut(s) 437, 1018
BamHI GGATCC 2 cut(s) 472, 1531
BanI GGYRCC 4 cut(s) 590, 802, 2072, 2559
BanII GRGCYC 2 cut(s) 570, 2085
BarI GAAGNNNNNNTAC 2 cut(s) 225, 257
BbsI GAAGAC 1 cut(s) 2684
BceAI ACGGC 2 cut(s) 2536, 3820
BcgI CGANNNNNNTGC 6 cut(s) 254, 288, 2530, 2564, 2685, 2719
BciT130I CCWGG 6 cut(s) 470, 632, 1469, 1752, 1880, 2353
BciVI GTATCC 1 cut(s) 3283
BcnI CCSGG 7 cut(s) 722, 1179, 2078, 2408, 2611, 2631, 2632
BfaI CTAG 1 cut(s) 953
BfmI CTRYAG 7 cut(s) 483, 555, 1278, 3093, 3099, 3422, 3602
BfoI RGCGCY 1 cut(s) 1254
BfuAI ACCTGC 2 cut(s) 1502, 2188
BfuI GTATCC 1 cut(s) 3283
BglI GCCNNNNNGGC 1 cut(s) 843
BglII AGATCT 1 cut(s) 3798
BmcAI AGTACT 3 cut(s) 234, 785, 1650
Bme18I GGWCC 4 cut(s) 297, 1321, 1491, 2947
BmeRI GACNNNNNGTC 1 cut(s) 3534
BmeT110I CYCGRG 3 cut(s) 2268, 2630, 3113
BmgBI CACGTC 1 cut(s) 3525
BmgT120I GGNCC 9 cut(s) 297, 1267, 1321, 1491, 1872, 1978, 2382, 2947, 3619
BmrI ACTGGG 4 cut(s) 301, 420, 1207, 2012
BmuI ACTGGG 4 cut(s) 301, 420, 1207, 2012
BoxI GACNNNNGTC 3 cut(s) 260, 658, 3833
BpiI GAAGAC 1 cut(s) 2684
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 7 cut(s) 365, 756, 1734, 1757, 1955, 2448, 2667
Bpu14I TTCGAA 3 cut(s) 378, 2303, 2705
BpuEI CTTGAG 2 cut(s) 605, 3461
BpuMI CCSGG 7 cut(s) 722, 1179, 2078, 2408, 2611, 2631, 2632
BsaBI GATNNNNATC 4 cut(s) 909, 1530, 2458, 2687
BsaHI GRCGYC 3 cut(s) 696, 1739, 2411
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 8 cut(s) 300, 1432, 1879, 2068, 2076, 2226, 2630, 3183
BsaWI WCCGGW 3 cut(s) 336, 1558, 2517
BsaXI ACNNNNNCTCC 4 cut(s) 1799, 1829, 3674, 3704
Bse118I RCCGGY 8 cut(s) 974, 1802, 1856, 2051, 2344, 2369, 2932, 3629
Bse1I ACTGG 9 cut(s) 296, 415, 739, 781, 1202, 1774, 1981, 2018, 2149
Bse3DI GCAATG 6 cut(s) 568, 1105, 1170, 2914, 3123, 3693
Bse8I GATNNNNATC 4 cut(s) 909, 1530, 2458, 2687
BseAI TCCGGA 1 cut(s) 336
BseBI CCWGG 6 cut(s) 470, 632, 1469, 1752, 1880, 2353
BseDI CCNNGG 8 cut(s) 300, 1432, 1879, 2068, 2076, 2226, 2630, 3183
BseJI GATNNNNATC 4 cut(s) 909, 1530, 2458, 2687
BseMI GCAATG 6 cut(s) 568, 1105, 1170, 2914, 3123, 3693
BseMII CTCAG 7 cut(s) 729, 847, 1395, 2409, 2849, 3123, 3309
BseNI ACTGG 9 cut(s) 296, 415, 739, 781, 1202, 1774, 1981, 2018, 2149
BseRI GAGGAG 7 cut(s) 1643, 1777, 1814, 2256, 2664, 2792, 3131
BseX3I CGGCCG 2 cut(s) 2342, 2367
BseYI CCCAGC 4 cut(s) 1108, 1975, 2754, 2960
Bsh1236I CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
Bsh1285I CGRYCG 6 cut(s) 1999, 2056, 2341, 2345, 2370, 3837
BshNI GGYRCC 4 cut(s) 590, 802, 2072, 2559
BsiEI CGRYCG 6 cut(s) 1999, 2056, 2341, 2345, 2370, 3837
BsiHKCI CYCGRG 3 cut(s) 2268, 2630, 3113
BsmBI CGTCTC 2 cut(s) 1693, 3754
BsmI GAATGC 3 cut(s) 1444, 1451, 3775
Bso31I GGTCTC 1 cut(s) 966
BsoBI CYCGRG 3 cut(s) 2268, 2630, 3113
Bsp119I TTCGAA 3 cut(s) 378, 2303, 2705
Bsp1286I GDGCHC 2 cut(s) 570, 2085
Bsp13I TCCGGA 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 789
Bsp19I CCATGG 1 cut(s) 300
BspCNI CTCAG 7 cut(s) 728, 846, 1394, 2410, 2848, 3124, 3310
BspEI TCCGGA 1 cut(s) 336
BspFNI CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
BspHI TCATGA 1 cut(s) 252
BspMAI CTGCAG 6 cut(s) 487, 1282, 3097, 3103, 3426, 3606
BspMI ACCTGC 2 cut(s) 1502, 2188
BspQI GCTCTTC 1 cut(s) 1478
BspT104I TTCGAA 3 cut(s) 378, 2303, 2705
BspT107I GGYRCC 4 cut(s) 590, 802, 2072, 2559
BspTNI GGTCTC 1 cut(s) 966
BsrBI CCGCTC 2 cut(s) 404, 1815
BsrDI GCAATG 6 cut(s) 568, 1105, 1170, 2914, 3123, 3693
BsrFI RCCGGY 8 cut(s) 974, 1802, 1856, 2051, 2344, 2369, 2932, 3629
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 9 cut(s) 296, 415, 739, 781, 1202, 1774, 1981, 2018, 2149
BssAI RCCGGY 8 cut(s) 974, 1802, 1856, 2051, 2344, 2369, 2932, 3629
BssECI CCNNGG 8 cut(s) 300, 1432, 1879, 2068, 2076, 2226, 2630, 3183
BssNI GRCGYC 3 cut(s) 696, 1739, 2411
BssT1I CCWWGG 4 cut(s) 300, 1432, 2226, 3183
Bst2UI CCWGG 6 cut(s) 470, 632, 1469, 1752, 1880, 2353
Bst4CI ACNGT 8 cut(s) 19, 679, 814, 1590, 2989, 3556, 3686, 3835
Bst6I CTCTTC 9 cut(s) 62, 1052, 1478, 1716, 2505, 3043, 3178, 3499, 3785
BstACI GRCGYC 3 cut(s) 696, 1739, 2411
BstAUI TGTACA 1 cut(s) 789
BstBI TTCGAA 3 cut(s) 378, 2303, 2705
BstDEI CTNAG 9 cut(s) 156, 715, 833, 1381, 2325, 2418, 2835, 3132, 3318
BstDSI CCRYGG 1 cut(s) 300
BstEII GGTNACC 2 cut(s) 1519, 2928
BstFNI CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
BstH2I RGCGCY 1 cut(s) 1254
BstHHI GCGC 4 cut(s) 1253, 2851, 2969, 3656
BstMCI CGRYCG 6 cut(s) 1999, 2056, 2341, 2345, 2370, 3837
BstNI CCWGG 6 cut(s) 470, 632, 1469, 1752, 1880, 2353
BstNSI RCATGY 4 cut(s) 1088, 1462, 3160, 3824
BstPAI GACNNNNGTC 3 cut(s) 260, 658, 3833
BstPI GGTNACC 2 cut(s) 1519, 2928
BstSFI CTRYAG 7 cut(s) 483, 555, 1278, 3093, 3099, 3422, 3602
BstUI CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
BstV2I GAAGAC 1 cut(s) 2684
BstX2I RGATCY 6 cut(s) 472, 1531, 2331, 2425, 2459, 3798
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 6 cut(s) 472, 1531, 2331, 2425, 2459, 3798
BstZI CGGCCG 2 cut(s) 2342, 2367
BsuI GTATCC 1 cut(s) 3283
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 2 cut(s) 2032, 2877
BtrI CACGTC 1 cut(s) 3525
BtsIMutI CAGTG 7 cut(s) 15, 408, 1195, 1586, 3129, 3315, 3691
BveI ACCTGC 2 cut(s) 1502, 2188
CciI TCATGA 1 cut(s) 252
CfoI GCGC 4 cut(s) 1253, 2851, 2969, 3656
Cfr10I RCCGGY 8 cut(s) 974, 1802, 1856, 2051, 2344, 2369, 2932, 3629
Cfr13I GGNCC 9 cut(s) 297, 1267, 1321, 1491, 1872, 1978, 2382, 2947, 3619
Cfr9I CCCGGG 1 cut(s) 2630
CseI GACGC 3 cut(s) 1728, 2017, 2400
CsiI ACCWGGT 1 cut(s) 1467
Csp6I GTAC 7 cut(s) 233, 583, 784, 790, 1649, 2313, 2560
CspCI CAANNNNNGTGG 5 cut(s) 2269, 2304, 3241, 3276, 3835
CviQI GTAC 7 cut(s) 233, 583, 784, 790, 1649, 2313, 2560
DdeI CTNAG 9 cut(s) 156, 715, 833, 1381, 2325, 2418, 2835, 3132, 3318
DrdI GACNNNNNNGTC 1 cut(s) 2018
DriI GACNNNNNGTC 1 cut(s) 3534
DseDI GACNNNNNNGTC 1 cut(s) 2018
EaeI YGGCCR 6 cut(s) 399, 435, 1016, 2342, 2367, 2796
EagI CGGCCG 2 cut(s) 2342, 2367
Eam1104I CTCTTC 9 cut(s) 62, 1052, 1478, 1716, 2505, 3043, 3178, 3499, 3785
Eam1105I GACNNNNNGTC 1 cut(s) 3534
EarI CTCTTC 9 cut(s) 62, 1052, 1478, 1716, 2505, 3043, 3178, 3499, 3785
EciI GGCGGA 2 cut(s) 734, 1807
EclXI CGGCCG 2 cut(s) 2342, 2367
Eco130I CCWWGG 4 cut(s) 300, 1432, 2226, 3183
Eco24I GRGCYC 2 cut(s) 570, 2085
Eco31I GGTCTC 1 cut(s) 966
Eco47I GGWCC 4 cut(s) 297, 1321, 1491, 2947
Eco52I CGGCCG 2 cut(s) 2342, 2367
Eco57I CTGAAG 5 cut(s) 771, 1784, 3024, 3126, 3809
Eco88I CYCGRG 3 cut(s) 2268, 2630, 3113
Eco91I GGTNACC 2 cut(s) 1519, 2928
EcoO109I RGGNCCY 2 cut(s) 1267, 3619
EcoO65I GGTNACC 2 cut(s) 1519, 2928
EcoRI GAATTC 3 cut(s) 48, 2299, 2305
EcoRII CCWGG 6 cut(s) 468, 630, 1467, 1750, 1878, 2351
EcoT14I CCWWGG 4 cut(s) 300, 1432, 2226, 3183
EcoT22I ATGCAT 1 cut(s) 1453
EcoT38I GRGCYC 2 cut(s) 570, 2085
ErhI CCWWGG 4 cut(s) 300, 1432, 2226, 3183
Esp3I CGTCTC 2 cut(s) 1693, 3754
FauI CCCGC 5 cut(s) 327, 2033, 2443, 3211, 3714
FblI GTMKAC 4 cut(s) 699, 1995, 2056, 3837
FriOI GRGCYC 2 cut(s) 570, 2085
FspBI CTAG 1 cut(s) 953
FspI TGCGCA 2 cut(s) 2968, 3655
GlaI GCGC 4 cut(s) 1252, 2850, 2968, 3655
GsaI CCCAGC 4 cut(s) 1112, 1979, 2758, 2964
GsuI CTGGAG 7 cut(s) 365, 756, 1734, 1757, 1955, 2448, 2667
HaeII RGCGCY 1 cut(s) 1254
HgaI GACGC 3 cut(s) 1728, 2017, 2400
HhaI GCGC 4 cut(s) 1253, 2851, 2969, 3656
Hin1I GRCGYC 3 cut(s) 696, 1739, 2411
Hin6I GCGC 4 cut(s) 1251, 2849, 2967, 3654
HinP1I GCGC 4 cut(s) 1251, 2849, 2967, 3654
HincII GTYRAC 4 cut(s) 817, 1996, 2057, 3838
HindII GTYRAC 4 cut(s) 817, 1996, 2057, 3838
HindIII AAGCTT 2 cut(s) 1766, 3066
HpaI GTTAAC 1 cut(s) 817
HpyCH4III ACNGT 8 cut(s) 19, 679, 814, 1590, 2989, 3556, 3686, 3835
HpyCH4IV ACGT 5 cut(s) 581, 696, 1890, 1992, 3524
HpyF3I CTNAG 9 cut(s) 156, 715, 833, 1381, 2325, 2418, 2835, 3132, 3318
HpySE526I ACGT 5 cut(s) 581, 696, 1890, 1992, 3524
Hsp92I GRCGYC 3 cut(s) 696, 1739, 2411
HspAI GCGC 4 cut(s) 1251, 2849, 2967, 3654
Kpn2I TCCGGA 1 cut(s) 336
KpnI GGTACC 1 cut(s) 2563
KroI GCCGGC 3 cut(s) 974, 1856, 2344
KroNI GCCGGC 3 cut(s) 976, 1858, 2346
KspAI GTTAAC 1 cut(s) 817
LguI GCTCTTC 1 cut(s) 1478
MabI ACCWGGT 1 cut(s) 1467
MaeI CTAG 1 cut(s) 953
MaeII ACGT 5 cut(s) 581, 696, 1890, 1992, 3524
MbiI CCGCTC 2 cut(s) 404, 1815
MfeI CAATTG 2 cut(s) 2577, 3267
MflI RGATCY 6 cut(s) 472, 1531, 2331, 2425, 2459, 3798
MhlI GDGCHC 2 cut(s) 570, 2085
MlsI TGGCCA 2 cut(s) 437, 1018
MluNI TGGCCA 2 cut(s) 437, 1018
MlyI GAGTC 9 cut(s) 707, 755, 1898, 2006, 2654, 2771, 2827, 3143, 3753
MmeI TCCRAC 7 cut(s) 982, 1887, 1926, 2014, 2118, 2169, 2374
Mox20I TGGCCA 2 cut(s) 437, 1018
Mph1103I ATGCAT 1 cut(s) 1453
MroI TCCGGA 1 cut(s) 336
MroNI GCCGGC 3 cut(s) 974, 1856, 2344
MroXI GAANNNNTTC 3 cut(s) 116, 374, 1061
MscI TGGCCA 2 cut(s) 437, 1018
MseI TTAA 8 cut(s) 179, 816, 900, 1332, 2495, 2858, 3152, 3243
MslI CAYNNNNRTG 3 cut(s) 257, 926, 1733
Msp20I TGGCCA 2 cut(s) 437, 1018
MspA1I CMGCKG 8 cut(s) 1277, 1975, 2587, 2964, 3098, 3104, 3659, 3701
MunI CAATTG 2 cut(s) 2577, 3267
Mva1269I GAATGC 3 cut(s) 1444, 1451, 3775
MvaI CCWGG 6 cut(s) 470, 632, 1469, 1752, 1880, 2353
MvnI CGCG 5 cut(s) 1040, 1055, 2028, 2851, 3516
NaeI GCCGGC 3 cut(s) 976, 1858, 2346
NciI CCSGG 7 cut(s) 722, 1179, 2078, 2408, 2611, 2631, 2632
NcoI CCATGG 1 cut(s) 300
NgoMIV GCCGGC 3 cut(s) 974, 1856, 2344
NmeAIII GCCGAG 3 cut(s) 2049, 2081, 3351
NmuCI GTSAC 9 cut(s) 201, 508, 660, 1028, 1519, 1798, 1940, 2928, 3536
NsbI TGCGCA 2 cut(s) 2968, 3655
NsiI ATGCAT 1 cut(s) 1453
NspI RCATGY 4 cut(s) 1088, 1462, 3160, 3824
NspV TTCGAA 3 cut(s) 378, 2303, 2705
OliI CACNNNNGTG 1 cut(s) 926
PaeI GCATGC 1 cut(s) 1088
PaeR7I CTCGAG 2 cut(s) 2268, 3113
PagI TCATGA 1 cut(s) 252
PciI ACATGT 2 cut(s) 1458, 3820
PciSI GCTCTTC 1 cut(s) 1478
PcsI WCGNNNNNNNCGW 4 cut(s) 384, 1860, 1992, 2001
PctI GAATGC 3 cut(s) 1444, 1451, 3775
PdiI GCCGGC 3 cut(s) 976, 1858, 2346
PdmI GAANNNNTTC 3 cut(s) 116, 374, 1061
PflFI GACNNNGTC 2 cut(s) 2276, 3554
PflMI CCANNNNNTGG 2 cut(s) 734, 1025
PfoI TCCNGGA 4 cut(s) 630, 1177, 1750, 2609
Ple19I CGATCG 1 cut(s) 2341
PleI GAGTC 9 cut(s) 707, 755, 1898, 2006, 2654, 2770, 2827, 3142, 3752
PpsI GAGTC 9 cut(s) 707, 755, 1898, 2006, 2654, 2770, 2827, 3142, 3752
PscI ACATGT 2 cut(s) 1458, 3820
PshAI GACNNNNGTC 3 cut(s) 260, 658, 3833
PsiI TTATAA 1 cut(s) 2828
Psp6I CCWGG 6 cut(s) 468, 630, 1467, 1750, 1878, 2351
PspEI GGTNACC 2 cut(s) 1519, 2928
PspFI CCCAGC 4 cut(s) 1108, 1975, 2754, 2960
PspGI CCWGG 6 cut(s) 468, 630, 1467, 1750, 1878, 2351
PspPI GGNCC 9 cut(s) 297, 1267, 1321, 1491, 1872, 1978, 2382, 2947, 3619
PspXI VCTCGAGB 1 cut(s) 3113
PstI CTGCAG 6 cut(s) 487, 1282, 3097, 3103, 3426, 3606
PsuI RGATCY 6 cut(s) 472, 1531, 2331, 2425, 2459, 3798
PsyI GACNNNGTC 2 cut(s) 2276, 3554
PvuI CGATCG 1 cut(s) 2341
PvuII CAGCTG 7 cut(s) 1277, 1975, 2964, 3098, 3104, 3659, 3701
RsaI GTAC 7 cut(s) 234, 584, 785, 791, 1650, 2314, 2561
RsaNI GTAC 7 cut(s) 233, 583, 784, 790, 1649, 2313, 2560
RseI CAYNNNNRTG 3 cut(s) 257, 926, 1733
SalI GTCGAC 3 cut(s) 1994, 2055, 3836
SapI GCTCTTC 1 cut(s) 1478
SaqAI TTAA 8 cut(s) 179, 816, 900, 1332, 2495, 2858, 3152, 3243
Sau96I GGNCC 9 cut(s) 297, 1267, 1321, 1491, 1872, 1978, 2382, 2947, 3619
ScaI AGTACT 3 cut(s) 234, 785, 1650
SchI GAGTC 9 cut(s) 707, 755, 1898, 2006, 2654, 2771, 2827, 3143, 3753
SduI GDGCHC 2 cut(s) 570, 2085
SexAI ACCWGGT 1 cut(s) 1467
SfcI CTRYAG 7 cut(s) 483, 555, 1278, 3093, 3099, 3422, 3602
Sfr274I CTCGAG 2 cut(s) 2268, 3113
SfuI TTCGAA 3 cut(s) 378, 2303, 2705
SgrAI CRCCGGYG 1 cut(s) 1802
SinI GGWCC 4 cut(s) 297, 1321, 1491, 2947
SlaI CTCGAG 2 cut(s) 2268, 3113
SmaI CCCGGG 1 cut(s) 2632
SmiMI CAYNNNNRTG 3 cut(s) 257, 926, 1733
SmlI CTYRAG 4 cut(s) 620, 2268, 3113, 3476
SmoI CTYRAG 4 cut(s) 620, 2268, 3113, 3476
SphI GCATGC 1 cut(s) 1088
SspI AATATT 1 cut(s) 396
SspMI CTAG 1 cut(s) 953
StyI CCWWGG 4 cut(s) 300, 1432, 2226, 3183
TaaI ACNGT 8 cut(s) 19, 679, 814, 1590, 2989, 3556, 3686, 3835
TaiI ACGT 5 cut(s) 584, 699, 1893, 1995, 3527
TaqII GACCGA 1 cut(s) 1538
TatI WGTACW 5 cut(s) 232, 783, 789, 1648, 2312
TauI GCSGC 2 cut(s) 404, 2540
Tru1I TTAA 8 cut(s) 179, 816, 900, 1332, 2495, 2858, 3152, 3243
Tru9I TTAA 8 cut(s) 179, 816, 900, 1332, 2495, 2858, 3152, 3243
TscAI CASTG 7 cut(s) 22, 415, 1202, 1593, 3136, 3322, 3691
TseFI GTSAC 9 cut(s) 201, 508, 660, 1028, 1519, 1798, 1940, 2928, 3536
Tsp45I GTSAC 9 cut(s) 201, 508, 660, 1028, 1519, 1798, 1940, 2928, 3536
TspGWI ACGGA 5 cut(s) 369, 1528, 1675, 2030, 3555
TspMI CCCGGG 1 cut(s) 2630
TspRI CASTG 7 cut(s) 22, 415, 1202, 1593, 3136, 3322, 3691
Tth111I GACNNNGTC 2 cut(s) 2276, 3554
Van91I CCANNNNNTGG 2 cut(s) 734, 1025
VpaK11BI GGWCC 4 cut(s) 297, 1321, 1491, 2947
XapI RAATTY 5 cut(s) 48, 2299, 2305, 2605, 3309
XceI RCATGY 4 cut(s) 1088, 1462, 3160, 3824
XcmI CCANNNNNNNNNTGG 1 cut(s) 2161
XhoI CTCGAG 2 cut(s) 2268, 3113
XmaI CCCGGG 1 cut(s) 2630
XmiI GTMKAC 4 cut(s) 699, 1995, 2056, 3837
XmnI GAANNNNTTC 3 cut(s) 116, 374, 1061
XspI CTAG 1 cut(s) 953
ZraI GACGTC 1 cut(s) 697
ZrmI AGTACT 3 cut(s) 234, 785, 1650
Zsp2I ATGCAT 1 cut(s) 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.