MD17G1097200.v1.1

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011282
Physical Location & Seq
Reverse (-)
8234095 .. 8235261
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1097200.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGCGATTTACCTGTCCAATAAGCAGCAGGAGCAGGTTGTGAGGAATGACAACAAAGAAGCCAAGATTGAGAATGCACGACCATCAAGGCAGCCCCTTGCTCCTCGAGACAATGTCAATACGGGAGGGAATTTGAATTCAAATTCAATTTCGGATGAGGCTAAGAAAATCTCAGATCGGCCGACTTCCAGGTTTTCGTCTCCGGCAGGTGCAAAGATGTCTATTTCTGTCGGGAAGAAGAACCCTGCATCGGTCGAGAGAGATCCATCGCTGGCAGGAAAAGGAAAGAGATCGGGATATCCAACCCTTTCGAAATGCGTGGTTCTAAGCTTAATTGTGGCGAAGGAAGAGGATAGGAAGGCGTCGAAAGAGATGGCGATTATAGTGACGTCAAGGTACCGACAGCCGTCCCCAATGGCAGGTATAGTTGCAGGGATTTCGAAGGTTTCGGAAGCCCTCATTGGGTCTGGAAAGGGTAATCGAAAGGGCTGGGATCAGTCGCCGGCGGTGGAGCAGAAAGAGAAGCATGCGGCGAAGAACAAACCAGATTTGCAGTCATTTATAAGGACTCAGGCTGCCCTTGCGCGACGATTAAGCGATGCGAAAGGTGGAATTCCGAACGGCGGTGATGATTCTTCCAGCAATGAGAAAACGAAATCAGGATCACTGAAAGATTTGGTGGTCCAAGAGAGACCCAGCTGTGCAGCAGCTCCAGGCATCACTGTTCGTGAAAAAAAAAAATGGACTGATGAAAGTGTCTCACTTGATGCAGTCTCTTCAGACCTTGCAAGGCTTGCAAAGGAAGCTTTGCAGAGGAAAGTTCTAGCTTCTGCAGCCGCAGCTGAAGCTCTGGAGGGGGCTATTACTACTGAGTCTCTAGTGAGGAAATTAAGCATGTTTACAGAACTCGCTTCTACATCCAAGGTTGGGAGCCCTCTACCCGCCATTGACCGCTTCTTTTCGATCTATGATGAAGTTGTTAAATTAACCACACTTACTGAATCAATTGCTAGTAACCGCAATTCAGATACATGTTACAATGATCAAGTCTCCCCATCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

355

Amino Acids

37.9

Weight (kDa)

9.58

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6857 PF21647 244 - 342 9.9e-33 Domain of unknown function (DUF6857)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13370
fragaria_vesca FvH4_1g05440 FvH4_1g05440
malus_domestica MD02G1062500.v1.1 MD15G1190300.v1.1 MD17G1097200.v1.1
prunus_persica Prupe.7G226800_v2.0.a1
pyrus_communis pycom02g04340 pycom15g17050
rosa_chinensis RchiOBHm_Chr2g0091101
rosa_laevigata RLG00000016180
rosa_multiflora Rmu_sc0004851.1_g000007 Rmu_sc0005171.1_g000002
rosa_roxburghii Rroxscaffold_2G00150540
rosa_rugosa Rorug02G0013000
rosa_samantha Rh2AG059200 Rh2BG057600 Rh2CG059500 Rh2DG058600
rosa_wichuraiana Rw2G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
AarI CACCTGC 1 cut(s) 197
AatII GACGTC 1 cut(s) 392
Acc36I ACCTGC 3 cut(s) 25, 197, 410
Acc65I GGTACC 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 396
AccII CGCG 1 cut(s) 586
AciI CCGC 7 cut(s) 506, 530, 624, 837, 942, 952, 1018
AclWI GGATC 3 cut(s) 257, 501, 670
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 4 cut(s) 130, 136, 142, 612
AcuI CTGAAG 2 cut(s) 762, 864
AcyI GRCGYC 2 cut(s) 362, 389
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 5 cut(s) 250, 419, 462, 623, 927
AflIII ACRYGT 1 cut(s) 1031
AgsI TTSAA 3 cut(s) 136, 141, 147
AjnI CCWGG 2 cut(s) 188, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 7 cut(s) 330, 699, 710, 806, 827, 842, 848
AluI AGCT 7 cut(s) 330, 699, 710, 806, 827, 842, 848
Alw26I GTCTC 7 cut(s) 102, 204, 685, 763, 778, 879, 1054
AlwI GGATC 3 cut(s) 257, 501, 670
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 7 cut(s) 25, 91, 575, 704, 707, 833, 839
ApoI RAATTY 4 cut(s) 130, 136, 142, 612
Asp718I GGTACC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 1 cut(s) 638
AsuII TTCGAA 2 cut(s) 311, 440
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 935
BbvI GCAGC 7 cut(s) 37, 103, 562, 716, 719, 845, 851
BccI CCATC 4 cut(s) 91, 274, 367, 1063
BceAI ACGGC 2 cut(s) 391, 637
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BciT130I CCWGG 2 cut(s) 190, 714
BclI TGATCA 1 cut(s) 1042
BcoDI GTCTC 7 cut(s) 102, 204, 685, 763, 778, 879, 1054
BfaI CTAG 4 cut(s) 824, 878, 1011, 1063
BfmI CTRYAG 1 cut(s) 831
BfuAI ACCTGC 3 cut(s) 25, 197, 410
BisI GCNGC 9 cut(s) 26, 92, 531, 576, 705, 708, 834, 837, 840
BlsI GCNGC 9 cut(s) 27, 93, 532, 577, 706, 709, 835, 838, 841
Bme1390I CCNGG 2 cut(s) 190, 714
Bme18I GGWCC 1 cut(s) 682
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 2 cut(s) 398, 932
BmrFI CCNGG 2 cut(s) 190, 714
BmsI GCATC 4 cut(s) 257, 589, 726, 757
BoxI GACNNNNGTC 1 cut(s) 406
BpmI CTGGAG 2 cut(s) 696, 872
Bpu14I TTCGAA 2 cut(s) 311, 440
BsaHI GRCGYC 2 cut(s) 362, 389
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 921
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 419, 462, 623, 927
Bse118I RCCGGY 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 649
BseBI CCWGG 2 cut(s) 190, 714
BseDI CCNNGG 1 cut(s) 921
BseGI GGATG 3 cut(s) 160, 917, 1055
BseLI CCNNNNNNNGG 5 cut(s) 250, 419, 462, 623, 927
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 3 cut(s) 186, 584, 861
BseRI GAGGAG 1 cut(s) 93
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 7 cut(s) 37, 103, 562, 716, 719, 845, 851
BseYI CCCAGC 2 cut(s) 489, 695
BsgI GTGCAG 1 cut(s) 723
Bsh1236I CGCG 1 cut(s) 586
Bsh1285I CGRYCG 2 cut(s) 182, 255
BshFI GGCC 1 cut(s) 181
BshNI GGYRCC 1 cut(s) 396
BsiEI CGRYCG 2 cut(s) 182, 255
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 2 cut(s) 203, 503
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 5 cut(s) 250, 419, 462, 623, 927
BsmAI GTCTC 7 cut(s) 102, 204, 685, 763, 778, 879, 1054
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 394
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 105
Bsp119I TTCGAA 2 cut(s) 311, 440
Bsp1286I GDGCHC 1 cut(s) 935
Bsp143I GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
BspACI CCGC 7 cut(s) 506, 530, 624, 837, 942, 952, 1018
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 3 cut(s) 185, 583, 862
BspFNI CGCG 1 cut(s) 586
BspLI GGNNCC 2 cut(s) 398, 932
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 3 cut(s) 25, 197, 410
BspPI GGATC 3 cut(s) 257, 501, 670
BspT104I TTCGAA 2 cut(s) 311, 440
BspT107I GGYRCC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 649
BsrFI RCCGGY 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 1 cut(s) 921
BssMI GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
BssNI GRCGYC 2 cut(s) 362, 389
BssT1I CCWWGG 1 cut(s) 921
Bst2UI CCWGG 2 cut(s) 190, 714
Bst4CI ACNGT 1 cut(s) 724
Bst6I CTCTTC 2 cut(s) 342, 781
BstACI GRCGYC 2 cut(s) 362, 389
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 2 cut(s) 311, 440
BstC8I GCNNGC 4 cut(s) 273, 504, 528, 795
BstDEI CTNAG 5 cut(s) 162, 172, 326, 570, 870
BstF5I GGATG 3 cut(s) 160, 917, 1055
BstFNI CGCG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 7 cut(s) 178, 265, 293, 496, 665, 966, 1045
BstMAI GTCTC 7 cut(s) 102, 204, 685, 763, 778, 879, 1054
BstMBI GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
BstMCI CGRYCG 2 cut(s) 182, 255
BstMWI GCNNNNNNNGC 7 cut(s) 31, 581, 794, 803, 833, 839, 845
BstNI CCWGG 2 cut(s) 190, 714
BstNSI RCATGY 3 cut(s) 530, 898, 1035
BstPAI GACNNNNGTC 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 188, 712
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 7 cut(s) 37, 103, 562, 716, 719, 845, 851
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 1 cut(s) 181
BtgZI GCGATG 2 cut(s) 252, 612
BtsCI GGATG 3 cut(s) 160, 917, 1055
BtsIMutI CAGTG 2 cut(s) 665, 720
BveI ACCTGC 3 cut(s) 25, 197, 410
Cac8I GCNNGC 4 cut(s) 273, 504, 528, 795
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 682
CseI GACGC 1 cut(s) 351
Csp6I GTAC 1 cut(s) 397
CviAII CATG 3 cut(s) 527, 895, 1032
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 5 cut(s) 162, 172, 326, 570, 870
DpnI GATC 7 cut(s) 177, 264, 292, 495, 664, 965, 1044
DpnII GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
EaeI YGGCCR 1 cut(s) 179
EagI CGGCCG 1 cut(s) 179
Eam1104I CTCTTC 2 cut(s) 342, 781
EarI CTCTTC 2 cut(s) 342, 781
EclXI CGGCCG 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 935
Eco31I GGTCTC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 682
Eco52I CGGCCG 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 762, 864
Eco88I CYCGRG 1 cut(s) 105
EcoRI GAATTC 2 cut(s) 136, 612
EcoRII CCWGG 2 cut(s) 188, 712
EcoRV GATATC 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 921
EcoT38I GRGCYC 1 cut(s) 935
ErhI CCWWGG 1 cut(s) 921
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 3 cut(s) 530, 898, 1035
FaiI YATR 7 cut(s) 383, 425, 528, 563, 896, 969, 1033
FaqI GGGAC 1 cut(s) 394
FatI CATG 3 cut(s) 526, 894, 1031
FauI CCCGC 1 cut(s) 949
FbaI TGATCA 1 cut(s) 1042
Fnu4HI GCNGC 9 cut(s) 26, 92, 531, 576, 705, 708, 834, 837, 840
FokI GGATG 3 cut(s) 167, 904, 1042
FriOI GRGCYC 1 cut(s) 935
Fsp4HI GCNGC 9 cut(s) 26, 92, 531, 576, 705, 708, 834, 837, 840
FspBI CTAG 4 cut(s) 824, 878, 1011, 1063
GlaI GCGC 1 cut(s) 585
GluI GCNGC 9 cut(s) 26, 92, 531, 576, 705, 708, 834, 837, 840
GsaI CCCAGC 2 cut(s) 493, 699
GsuI CTGGAG 2 cut(s) 696, 872
HaeIII GGCC 1 cut(s) 181
HapII CCGG 2 cut(s) 203, 503
HgaI GACGC 1 cut(s) 351
HhaI GCGC 1 cut(s) 586
Hin1I GRCGYC 2 cut(s) 362, 389
Hin1II CATG 3 cut(s) 530, 898, 1035
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HindIII AAGCTT 2 cut(s) 328, 804
HinfI GANTC 4 cut(s) 568, 632, 872, 1001
HpaII CCGG 2 cut(s) 203, 503
HphI GGTGA 1 cut(s) 638
Hpy166II GTNNAC 1 cut(s) 900
Hpy188I TCNGA 6 cut(s) 154, 175, 451, 618, 781, 1027
Hpy188III TCNNGA 8 cut(s) 107, 232, 256, 294, 468, 660, 728, 851
Hpy8I GTNNAC 1 cut(s) 900
Hpy99I CGWCG 2 cut(s) 367, 591
HpyAV CCTTC 3 cut(s) 337, 352, 436
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 581, 794, 803, 833, 839, 845
HpyF3I CTNAG 5 cut(s) 162, 172, 326, 570, 870
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 2 cut(s) 362, 389
Hsp92II CATG 3 cut(s) 530, 898, 1035
HspAI GCGC 1 cut(s) 584
KpnI GGTACC 1 cut(s) 400
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
LmnI GCTCC 5 cut(s) 31, 106, 511, 715, 930
Lsp1109I GCAGC 7 cut(s) 37, 103, 562, 716, 719, 845, 851
LweI GCATC 4 cut(s) 257, 589, 726, 757
MaeI CTAG 4 cut(s) 824, 878, 1011, 1063
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 3 cut(s) 385, 1013, 1034
MalI GATC 7 cut(s) 177, 264, 292, 495, 664, 965, 1044
MboI GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
MboII GAAGA 6 cut(s) 247, 250, 359, 547, 627, 768
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 935
MlyI GAGTC 2 cut(s) 562, 881
MmeI TCCRAC 1 cut(s) 326
MreI CGCCGGCG 1 cut(s) 502
MroNI GCCGGC 1 cut(s) 502
MseI TTAA 5 cut(s) 332, 593, 890, 981, 986
MspA1I CMGCKG 2 cut(s) 699, 842
MspI CCGG 2 cut(s) 203, 503
MspR9I CCNGG 2 cut(s) 190, 714
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 2 cut(s) 190, 714
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 7 cut(s) 31, 581, 794, 803, 833, 839, 845
NaeI GCCGGC 1 cut(s) 504
NdeII GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
NgoMIV GCCGGC 1 cut(s) 502
NlaIII CATG 3 cut(s) 530, 898, 1035
NlaIV GGNNCC 2 cut(s) 398, 932
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 3 cut(s) 530, 898, 1035
NspV TTCGAA 2 cut(s) 311, 440
PaeI GCATGC 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 105
PaqCI CACCTGC 1 cut(s) 197
PciI ACATGT 1 cut(s) 1031
PctI GAATGC 1 cut(s) 79
PdiI GCCGGC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 632, 1001
PflFI GACNNNGTC 1 cut(s) 113
PkrI GCNGC 9 cut(s) 27, 93, 532, 577, 706, 709, 835, 838, 841
PleI GAGTC 2 cut(s) 562, 880
PpsI GAGTC 2 cut(s) 562, 880
PscI ACATGT 1 cut(s) 1031
PshAI GACNNNNGTC 1 cut(s) 406
PsiI TTATAA 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 188, 712
PspFI CCCAGC 2 cut(s) 489, 695
PspGI CCWGG 2 cut(s) 188, 712
PspN4I GGNNCC 2 cut(s) 398, 932
PspPI GGNCC 1 cut(s) 682
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 262
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 2 cut(s) 699, 842
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
SaqAI TTAA 5 cut(s) 332, 593, 890, 981, 986
SatI GCNGC 9 cut(s) 26, 92, 531, 576, 705, 708, 834, 837, 840
Sau3AI GATC 7 cut(s) 175, 262, 290, 493, 662, 963, 1042
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 2 cut(s) 562, 881
ScrFI CCNGG 2 cut(s) 190, 714
SduI GDGCHC 1 cut(s) 935
SfaNI GCATC 4 cut(s) 257, 589, 726, 757
SfcI CTRYAG 1 cut(s) 831
Sfr274I CTCGAG 1 cut(s) 105
SfuI TTCGAA 2 cut(s) 311, 440
SgrAI CRCCGGYG 1 cut(s) 502
SinI GGWCC 1 cut(s) 682
SlaI CTCGAG 1 cut(s) 105
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
SphI GCATGC 1 cut(s) 530
SsiI CCGC 7 cut(s) 506, 530, 624, 837, 942, 952, 1018
SspMI CTAG 4 cut(s) 824, 878, 1011, 1063
StyD4I CCNGG 2 cut(s) 188, 712
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 392
TaqI TCGA 7 cut(s) 106, 255, 311, 365, 440, 481, 962
TaqII GACCGA 1 cut(s) 241
TauI GCSGC 2 cut(s) 533, 839
TfiI GAWTC 2 cut(s) 632, 1001
Tru1I TTAA 5 cut(s) 332, 593, 890, 981, 986
Tru9I TTAA 5 cut(s) 332, 593, 890, 981, 986
TscAI CASTG 2 cut(s) 672, 727
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 7 cut(s) 25, 91, 575, 704, 707, 833, 839
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 2 cut(s) 765, 987
TspRI CASTG 2 cut(s) 672, 727
Tth111I GACNNNGTC 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 4 cut(s) 130, 136, 142, 612
XceI RCATGY 3 cut(s) 530, 898, 1035
XhoI CTCGAG 1 cut(s) 105
XspI CTAG 4 cut(s) 824, 878, 1011, 1063
ZraI GACGTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.