MD02G1062600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014631
Physical Location & Seq
Forward (+)
5019447 .. 5022835
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1062600.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGCGGGCAGGAAGGACGAGTCGGTGGTCCTGAACAGAACCAATGTTTTCGCGGCGCTCGGGAGCTTGAAGAAGAAGAAGAAGGGCGATAAGAGCTCGTCGAAGAGCTCGAAAGGGGGCGACGCAGCTCAGAACTCCGGGGAGGCGGAGAAGGAGGTGTTTTGGGCGCCGGCGCCCCTTATCGCGAAGTCGTGGGCCGATGTGGATGACGAGGACGATGATGACTACTATGCAACTACAGCTCCGCCGGAAATGGGTTGGGCCGGCGAGGATTCGAAGGCGGTGAAGGAGAGCGAGCTCGAAGCCGAGCATGCGGAGACCGTCGGTGAATTGGAAAGTGAAAGTGAGGAGGAAGGTCTTGATGAAGTTGATGACGTTGACGAGGAACATGAAAATGAACTTGAAGCCTCGGTCGAAACTGAGCCAGTTAAGAAGCTCCCTGAACCCTCTCCAGCCCCTAAGGAGACAGAAAGGCAGCTTTCTAAGAAGGAACTGAAGAAGAAGGGGCTTGAAGAACTTGAAGCAGTTCTTGCTGAGCTAGGATACCCTACCAAGACCGAGACCAGTGGCCAAGATGACTCCAATGGTATTGCCCAAGAGAAGAAAGCAGAGAATCAAAATGGCGATGTGGACAAGAAGGAGAATGTCACTGGGGAGAGCAAAGGTGCAAAAAAGAAGAAAAAGAAGGACAAGTCAGCGAAGGAGCCGAAAGAACCCCAGGACCAGCCTGATGGCACAAATGGTGGAAGTGCAGCAACAGATGAAAATGCTGGGACAGAGAAGGGAGAAGATGCATCTACTGGTGATGTTAAAGAGCGGCTGAAGAAAGTGGCGTCCATGAAGAAGAAGAAATCAAGCAAAGAGATGGATGCGGCCGCCCGAGCTGCTTCGAGTGAAGCTGCTGCCAGGAATGCTAGGCTAGCTGCAGCGAAGAAGAAAGAGAAGAATCACTACAACCAGCAGCCGGTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

35.0

Weight (kDa)

4.96

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014631)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05450
malus_domestica MD02G1062600.v1.1 MD15G1190400.v1.1
prunus_persica Prupe.7G226700_v2.0.a1
pyrus_communis pycom02g04330 pycom15g17060
rosa_chinensis RchiOBHm_Chr2g0091121
rosa_laevigata RLG00000016181
rosa_multiflora Rmu_sc0004851.1_g000008
rosa_roxburghii Rroxscaffold_2G00150530
rosa_rugosa Rorug02G0013100 Rorug02G0013200
rosa_samantha Rh2AG059400 Rh2BG057800 Rh2CG059600 Rh2DG058700
rosa_wichuraiana Rw2G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 166, 172
AccBSI CCGCTC 1 cut(s) 817
AccII CGCG 2 cut(s) 53, 185
AcoI YGGCCR 2 cut(s) 568, 873
AcuI CTGAAG 2 cut(s) 515, 842
AcyI GRCGYC 3 cut(s) 167, 173, 833
AfiI CCNNNNNNNGG 2 cut(s) 254, 964
AgsI TTSAA 4 cut(s) 70, 404, 512, 521
AjnI CCWGG 2 cut(s) 717, 905
Alw21I GWGCWC 3 cut(s) 98, 110, 300
Alw26I GTCTC 3 cut(s) 311, 458, 554
Ama87I CYCGRG 2 cut(s) 59, 879
AoxI GGCC 4 cut(s) 195, 261, 568, 873
ApeKI GCWGC 9 cut(s) 125, 475, 752, 884, 899, 902, 923, 926, 961
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 3 cut(s) 58, 169, 175
AspS9I GGNCC 4 cut(s) 28, 195, 261, 721
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 295, 338, 815
AsuII TTCGAA 1 cut(s) 275
AsuNHI GCTAGC 1 cut(s) 919
AvaI CYCGRG 2 cut(s) 59, 879
AvaII GGWCC 2 cut(s) 28, 721
AxyI CCTNAGG 1 cut(s) 459
BalI TGGCCA 1 cut(s) 570
BanI GGYRCC 2 cut(s) 166, 172
BanII GRGCYC 3 cut(s) 98, 110, 300
BarI GAAGNNNNNNTAC 2 cut(s) 935, 967
Bbv12I GWGCWC 3 cut(s) 98, 110, 300
BbvI GCAGC 8 cut(s) 137, 487, 764, 871, 886, 889, 910, 938
BccI CCATC 2 cut(s) 725, 859
BciT130I CCWGG 2 cut(s) 719, 907
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 3 cut(s) 311, 458, 554
BfaI CTAG 3 cut(s) 539, 915, 920
BfmI CTRYAG 2 cut(s) 237, 924
BfoI RGCGCY 3 cut(s) 59, 170, 176
BfuI GTATCC 1 cut(s) 536
BlpI GCTNAGC 1 cut(s) 534
Bme1390I CCNGG 3 cut(s) 139, 719, 907
Bme18I GGWCC 2 cut(s) 28, 721
BmeT110I CYCGRG 2 cut(s) 59, 879
BmgT120I GGNCC 4 cut(s) 28, 195, 261, 721
BmiI GGNNCC 3 cut(s) 168, 174, 705
BmrFI CCNGG 3 cut(s) 139, 719, 907
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 781, 803, 859
BmtI GCTAGC 1 cut(s) 923
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 139
BsaHI GRCGYC 3 cut(s) 167, 173, 833
BsaI GGTCTC 2 cut(s) 311, 554
BsaJI CCNNGG 3 cut(s) 138, 408, 717
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 964
Bse118I RCCGGY 3 cut(s) 169, 263, 964
Bse1I ACTGG 4 cut(s) 425, 564, 655, 805
Bse21I CCTNAGG 1 cut(s) 459
BseBI CCWGG 2 cut(s) 719, 907
BseDI CCNNGG 3 cut(s) 138, 408, 717
BseGI GGATG 2 cut(s) 211, 874
BseLI CCNNNNNNNGG 2 cut(s) 254, 964
BseMII CTCAG 3 cut(s) 143, 411, 525
BseNI ACTGG 4 cut(s) 425, 564, 655, 805
BseRI GAGGAG 1 cut(s) 362
BseX3I CGGCCG 1 cut(s) 873
BseXI GCAGC 8 cut(s) 137, 487, 764, 871, 886, 889, 910, 938
BseYI CCCAGC 1 cut(s) 770
BsgI GTGCAG 1 cut(s) 771
Bsh1236I CGCG 2 cut(s) 53, 185
Bsh1285I CGRYCG 2 cut(s) 414, 876
BshFI GGCC 4 cut(s) 197, 263, 570, 875
BshNI GGYRCC 2 cut(s) 166, 172
BsiEI CGRYCG 2 cut(s) 414, 876
BsiHKAI GWGCWC 3 cut(s) 98, 110, 300
BsiHKCI CYCGRG 2 cut(s) 59, 879
BsiSI CCGG 5 cut(s) 138, 170, 248, 264, 965
BslFI GGGAC 1 cut(s) 787
BslI CCNNNNNNNGG 2 cut(s) 254, 964
BsmAI GTCTC 3 cut(s) 311, 458, 554
BsmFI GGGAC 1 cut(s) 787
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 4 cut(s) 197, 263, 570, 875
Bso31I GGTCTC 2 cut(s) 311, 554
BsoBI CYCGRG 2 cut(s) 59, 879
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 3 cut(s) 98, 110, 300
Bsp1720I GCTNAGC 1 cut(s) 534
Bsp68I TCGCGA 1 cut(s) 185
BspANI GGCC 4 cut(s) 197, 263, 570, 875
BspCNI CTCAG 3 cut(s) 142, 412, 526
BspFNI CGCG 2 cut(s) 53, 185
BspLI GGNNCC 3 cut(s) 168, 174, 705
BspMAI CTGCAG 1 cut(s) 928
BspOI GCTAGC 1 cut(s) 923
BspQI GCTCTTC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 2 cut(s) 166, 172
BspTNI GGTCTC 2 cut(s) 311, 554
BsrBI CCGCTC 1 cut(s) 817
BsrFI RCCGGY 3 cut(s) 169, 263, 964
BsrI ACTGG 4 cut(s) 425, 564, 655, 805
BssAI RCCGGY 3 cut(s) 169, 263, 964
BssECI CCNNGG 3 cut(s) 138, 408, 717
BssNI GRCGYC 3 cut(s) 167, 173, 833
Bst2UI CCWGG 2 cut(s) 719, 907
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 3 cut(s) 167, 173, 833
BstAPI GCANNNNNTGC 2 cut(s) 530, 967
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 6 cut(s) 7, 171, 265, 296, 312, 921
BstDEI CTNAG 5 cut(s) 129, 420, 459, 483, 534
BstF5I GGATG 2 cut(s) 211, 874
BstFNI CGCG 2 cut(s) 53, 185
BstH2I RGCGCY 3 cut(s) 59, 170, 176
BstHHI GCGC 3 cut(s) 58, 169, 175
BstMAI GTCTC 3 cut(s) 311, 458, 554
BstMCI CGRYCG 2 cut(s) 414, 876
BstMWI GCNNNNNNNGC 9 cut(s) 93, 239, 311, 530, 881, 884, 911, 920, 967
BstNI CCWGG 2 cut(s) 719, 907
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 3 cut(s) 137, 717, 905
BstSFI CTRYAG 2 cut(s) 237, 924
BstUI CGCG 2 cut(s) 53, 185
BstV1I GCAGC 8 cut(s) 137, 487, 764, 871, 886, 889, 910, 938
BstXI CCANNNNNNTGG 1 cut(s) 731
BstZI CGGCCG 1 cut(s) 873
Bsu36I CCTNAGG 1 cut(s) 459
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 4 cut(s) 197, 263, 570, 875
BtgZI GCGATG 1 cut(s) 639
BtsCI GGATG 2 cut(s) 211, 874
BtsIMutI CAGTG 2 cut(s) 571, 648
BtuMI TCGCGA 1 cut(s) 185
Cac8I GCNNGC 6 cut(s) 7, 171, 265, 296, 312, 921
CciNI GCGGCCGC 1 cut(s) 873
CfoI GCGC 3 cut(s) 58, 169, 175
Cfr10I RCCGGY 3 cut(s) 169, 263, 964
Cfr13I GGNCC 4 cut(s) 28, 195, 261, 721
CseI GACGC 2 cut(s) 131, 822
CviAII CATG 3 cut(s) 311, 389, 838
DdeI CTNAG 5 cut(s) 129, 420, 459, 483, 534
DinI GGCGCC 2 cut(s) 168, 174
EaeI YGGCCR 2 cut(s) 568, 873
EagI CGGCCG 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 2 cut(s) 161, 234
Ecl136II GAGCTC 3 cut(s) 96, 108, 298
EclXI CGGCCG 1 cut(s) 873
Eco24I GRGCYC 3 cut(s) 98, 110, 300
Eco31I GGTCTC 2 cut(s) 311, 554
Eco47I GGWCC 2 cut(s) 28, 721
Eco52I CGGCCG 1 cut(s) 873
Eco53kI GAGCTC 3 cut(s) 96, 108, 298
Eco57I CTGAAG 2 cut(s) 515, 842
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 59, 879
EcoICRI GAGCTC 3 cut(s) 96, 108, 298
EcoRII CCWGG 2 cut(s) 717, 905
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 3 cut(s) 98, 110, 300
EgeI GGCGCC 2 cut(s) 168, 174
EheI GGCGCC 2 cut(s) 168, 174
FaeI CATG 3 cut(s) 314, 392, 841
FaiI YATR 4 cut(s) 231, 312, 390, 839
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 1 cut(s) 787
FatI CATG 3 cut(s) 310, 388, 837
FokI GGATG 2 cut(s) 218, 881
FriOI GRGCYC 3 cut(s) 98, 110, 300
FspBI CTAG 3 cut(s) 539, 915, 920
GlaI GCGC 3 cut(s) 57, 168, 174
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 3 cut(s) 59, 170, 176
HaeIII GGCC 4 cut(s) 197, 263, 570, 875
HapII CCGG 5 cut(s) 138, 170, 248, 264, 965
HgaI GACGC 2 cut(s) 131, 822
HhaI GCGC 3 cut(s) 58, 169, 175
Hin1I GRCGYC 3 cut(s) 167, 173, 833
Hin1II CATG 3 cut(s) 314, 392, 841
Hin6I GCGC 3 cut(s) 56, 167, 173
HinP1I GCGC 3 cut(s) 56, 167, 173
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 5 cut(s) 20, 272, 578, 613, 946
HpaII CCGG 5 cut(s) 138, 170, 248, 264, 965
HphI GGTGA 3 cut(s) 295, 338, 815
Hpy166II GTNNAC 2 cut(s) 379, 631
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 4 cut(s) 31, 61, 184, 359
Hpy8I GTNNAC 2 cut(s) 379, 631
Hpy99I CGWCG 3 cut(s) 103, 125, 326
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 233, 668, 752, 794, 926
HpyF10VI GCNNNNNNNGC 9 cut(s) 93, 239, 311, 530, 881, 884, 911, 920, 967
HpyF3I CTNAG 5 cut(s) 129, 420, 459, 483, 534
HpySE526I ACGT 1 cut(s) 375
Hsp92I GRCGYC 3 cut(s) 167, 173, 833
Hsp92II CATG 3 cut(s) 314, 392, 841
HspAI GCGC 3 cut(s) 56, 167, 173
KasI GGCGCC 2 cut(s) 166, 172
KroI GCCGGC 2 cut(s) 169, 263
KroNI GCCGGC 2 cut(s) 171, 265
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 4 cut(s) 63, 247, 441, 703
Lsp1109I GCAGC 8 cut(s) 137, 487, 764, 871, 886, 889, 910, 938
LweI GCATC 3 cut(s) 781, 803, 859
MaeI CTAG 3 cut(s) 539, 915, 920
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 646
MbiI CCGCTC 1 cut(s) 817
MhlI GDGCHC 3 cut(s) 98, 110, 300
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 1 cut(s) 329
MluNI TGGCCA 1 cut(s) 570
Mly113I GGCGCC 2 cut(s) 167, 173
MlyI GAGTC 2 cut(s) 29, 572
MnlI CCTC 9 cut(s) 136, 148, 205, 262, 340, 343, 376, 418, 457
Mox20I TGGCCA 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 796
MreI CGCCGGCG 1 cut(s) 169
MroNI GCCGGC 2 cut(s) 169, 263
MroXI GAANNNNTTC 1 cut(s) 525
MscI TGGCCA 1 cut(s) 570
MseI TTAA 2 cut(s) 429, 810
MslI CAYNNNNRTG 1 cut(s) 393
Msp20I TGGCCA 1 cut(s) 570
MspI CCGG 5 cut(s) 138, 170, 248, 264, 965
MspR9I CCNGG 3 cut(s) 139, 719, 907
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 2 cut(s) 719, 907
MvnI CGCG 2 cut(s) 53, 185
MwoI GCNNNNNNNGC 9 cut(s) 93, 239, 311, 530, 881, 884, 911, 920, 967
NaeI GCCGGC 2 cut(s) 171, 265
NarI GGCGCC 2 cut(s) 167, 173
NciI CCSGG 1 cut(s) 139
NgoMIV GCCGGC 2 cut(s) 169, 263
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 3 cut(s) 314, 392, 841
NlaIV GGNNCC 3 cut(s) 168, 174, 705
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 873
NruI TCGCGA 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 1 cut(s) 314
NspV TTCGAA 1 cut(s) 275
PaeI GCATGC 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 916
PdiI GCCGGC 2 cut(s) 171, 265
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 3 cut(s) 272, 613, 946
PleI GAGTC 2 cut(s) 28, 572
PluTI GGCGCC 2 cut(s) 170, 176
PpsI GAGTC 2 cut(s) 28, 572
Psp124BI GAGCTC 3 cut(s) 98, 110, 300
Psp6I CCWGG 2 cut(s) 717, 905
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 2 cut(s) 717, 905
PspN4I GGNNCC 3 cut(s) 168, 174, 705
PspPI GGNCC 4 cut(s) 28, 195, 261, 721
PstI CTGCAG 1 cut(s) 928
RruI TCGCGA 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 393
SacI GAGCTC 3 cut(s) 98, 110, 300
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 429, 810
Sau96I GGNCC 4 cut(s) 28, 195, 261, 721
SchI GAGTC 2 cut(s) 29, 572
ScrFI CCNGG 3 cut(s) 139, 719, 907
SduI GDGCHC 3 cut(s) 98, 110, 300
SfaNI GCATC 3 cut(s) 781, 803, 859
SfcI CTRYAG 2 cut(s) 237, 924
SfoI GGCGCC 2 cut(s) 168, 174
SfuI TTCGAA 1 cut(s) 275
SgrAI CRCCGGYG 1 cut(s) 169
SinI GGWCC 2 cut(s) 28, 721
SmiMI CAYNNNNRTG 1 cut(s) 393
SphI GCATGC 1 cut(s) 314
Sse9I AATT 1 cut(s) 329
SspDI GGCGCC 2 cut(s) 166, 172
SspMI CTAG 3 cut(s) 539, 915, 920
SstI GAGCTC 3 cut(s) 98, 110, 300
StyD4I CCNGG 3 cut(s) 137, 717, 905
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 378
TaqI TCGA 6 cut(s) 101, 110, 275, 300, 414, 890
TaqII GACCGA 2 cut(s) 400, 572
TasI AATT 1 cut(s) 329
TauI GCSGC 4 cut(s) 56, 820, 875, 878
TfiI GAWTC 3 cut(s) 272, 613, 946
Tru1I TTAA 2 cut(s) 429, 810
Tru9I TTAA 2 cut(s) 429, 810
TscAI CASTG 2 cut(s) 571, 655
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 9 cut(s) 125, 475, 752, 884, 899, 902, 923, 926, 961
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 5 cut(s) 378, 405, 411, 777, 854
TspRI CASTG 2 cut(s) 571, 655
VpaK11BI GGWCC 2 cut(s) 28, 721
XceI RCATGY 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 3 cut(s) 539, 915, 920
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.