Rroxscaffold_2G00150530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014631
Physical Location & Seq
Reverse (-)
87879384 .. 87882834
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150530.1

Sequence Viewer

Length: 870 bp
ATGGCGGGAGGCGGGAGCAGGAAGGACGAGTCGGTGGTGCTGAACAGCACGAACGTGTTTGCGGCGCTGGGGAGCTTGAAGAAGAAGAAAAAGTCCGATAAGTCAAAGAGTGGGAAGAGCGGTCAGGCGGAGCCGGAGAAGGAGGTTTATTGGGCCCCGGCACCGCTCAACGTCAAGTCATGGGCCGATGTCGATGACGAGGATGATGACGACTACTTTGCGACTACTGCGCCGCCGGAATTGGGCTGGGCCGGCGGTGACTCCAAGGACCACAAGGAGAGTGAGCTGGAGCCTGAGCATGTGGAGGTCGGTGATTTGGTAAGTGACAGTGAAGAGGAAGGTCTCGATGATGTTGATGATGATGTGGACGAGGATCATGATAATGAACTTGAAGCACCAGTTGAAACTGAGACAGTTCTTGCTGAGCTAGGACTTGGCAAAAATGAGACCAGTGGCCACATTGAATCTGGAGATGGCCCCCAAGAGAAGAAATTAGAGAACCTAAATGGTGACATAGACAAGAAGGAAACCTCTGGGGAGAGCAAAAGTGCCAAAAAGAAGAAAAAGAAGGATAAGTCATCGAAGGAGTCGAGAGAATCCCAGGACCAGCCTGATGGCGTTGGAAATGCAGCAGCAGAAGAAACCACTGAAACTGAGAAGGTAGAAGATACGTCAACTGGTGATGTTAAAGAGCGGCTAAAGAAAGTGGCATCTATGAAGAAGAAGAAATCAAGCAAGGAAATGGATGCTGCAGCCCGAGCTGCTGCAAGTGAAGCTGCTGCCAGGAATGCTAGGCTGGCTTCAGCAAAGAAAAAGAGAAGAATCACTACAATCAGCAGCCGGTGCGATAAGCCAGCTATAAACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

31.14

Weight (kDa)

4.86

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014631)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05450
malus_domestica MD02G1062600.v1.1 MD15G1190400.v1.1
prunus_persica Prupe.7G226700_v2.0.a1
pyrus_communis pycom02g04330 pycom15g17060
rosa_chinensis RchiOBHm_Chr2g0091121
rosa_laevigata RLG00000016181
rosa_multiflora Rmu_sc0004851.1_g000008
rosa_roxburghii Rroxscaffold_2G00150530
rosa_rugosa Rorug02G0013100 Rorug02G0013200
rosa_samantha Rh2AG059400 Rh2BG057800 Rh2CG059600 Rh2DG058700
rosa_wichuraiana Rw2G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 160
AccBSI CCGCTC 3 cut(s) 120, 166, 694
AciI CCGC 9 cut(s) 5, 12, 62, 120, 128, 164, 233, 255, 694
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 454
AcuI CTGAAG 1 cut(s) 786
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 79, 392, 404, 464
AjnI CCWGG 2 cut(s) 600, 782
AleI CACNNNNGTG 1 cut(s) 53
AluBI AGCT 6 cut(s) 75, 286, 427, 761, 776, 857
AluI AGCT 6 cut(s) 75, 286, 427, 761, 776, 857
Alw26I GTCTC 3 cut(s) 347, 404, 440
AlwI GGATC 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 5 cut(s) 153, 183, 249, 454, 475
ApaI GGGCCC 1 cut(s) 157
ApeKI GCWGC 9 cut(s) 629, 632, 749, 752, 761, 764, 776, 779, 837
ArsI GACNNNNNNTTYG 2 cut(s) 200, 232
AspLEI GCGC 2 cut(s) 67, 232
AspS9I GGNCC 7 cut(s) 153, 154, 183, 249, 268, 476, 604
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 4 cut(s) 269, 323, 521, 692
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 2 cut(s) 268, 604
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 456
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 157
BarI GAAGNNNNNNTAC 2 cut(s) 811, 843
BbvI GCAGC 9 cut(s) 641, 644, 736, 748, 751, 763, 764, 766, 849
BccI CCATC 2 cut(s) 467, 608
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 2 cut(s) 602, 784
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 3 cut(s) 347, 404, 440
BfaI CTAG 2 cut(s) 428, 792
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 68
BlpI GCTNAGC 1 cut(s) 423
Bme1390I CCNGG 3 cut(s) 158, 602, 784
Bme18I GGWCC 2 cut(s) 268, 604
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 7 cut(s) 153, 154, 183, 249, 268, 476, 604
BmiI GGNNCC 6 cut(s) 132, 155, 156, 162, 291, 478
BmrFI CCNGG 3 cut(s) 158, 602, 784
BmsI GCATC 2 cut(s) 719, 736
BpmI CTGGAG 2 cut(s) 308, 489
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 158
BsaI GGTCTC 2 cut(s) 347, 440
BsaJI CCNNGG 3 cut(s) 156, 264, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse118I RCCGGY 2 cut(s) 251, 840
Bse1I ACTGG 3 cut(s) 398, 450, 682
BseBI CCWGG 2 cut(s) 602, 784
BseDI CCNNGG 3 cut(s) 156, 264, 600
BseGI GGATG 2 cut(s) 208, 751
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 4 cut(s) 285, 399, 414, 645
BseNI ACTGG 3 cut(s) 398, 450, 682
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 9 cut(s) 641, 644, 736, 748, 751, 763, 764, 766, 849
BseYI CCCAGC 2 cut(s) 67, 246
BshFI GGCC 5 cut(s) 155, 185, 251, 456, 477
BshNI GGYRCC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 5 cut(s) 134, 158, 236, 252, 841
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 3 cut(s) 347, 404, 440
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 5 cut(s) 155, 185, 251, 456, 477
Bso31I GGTCTC 2 cut(s) 347, 440
BsoBI CYCGRG 1 cut(s) 756
Bsp120I GGGCCC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 1 cut(s) 373
Bsp1720I GCTNAGC 1 cut(s) 423
BspACI CCGC 9 cut(s) 5, 12, 62, 120, 128, 164, 233, 255, 694
BspANI GGCC 5 cut(s) 155, 185, 251, 456, 477
BspCNI CTCAG 4 cut(s) 286, 400, 415, 646
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 6 cut(s) 132, 155, 156, 162, 291, 478
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 160
BspTNI GGTCTC 2 cut(s) 347, 440
BsrBI CCGCTC 3 cut(s) 120, 166, 694
BsrFI RCCGGY 2 cut(s) 251, 840
BsrI ACTGG 3 cut(s) 398, 450, 682
BssAI RCCGGY 2 cut(s) 251, 840
BssECI CCNNGG 3 cut(s) 156, 264, 600
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 602, 784
Bst4CI ACNGT 3 cut(s) 329, 415, 866
Bst6I CTCTTC 2 cut(s) 110, 327
BstAPI GCANNNNNTGC 1 cut(s) 843
BstC8I GCNNGC 3 cut(s) 253, 798, 855
BstDEI CTNAG 4 cut(s) 294, 408, 423, 654
BstF5I GGATG 2 cut(s) 208, 751
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 232
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 3 cut(s) 347, 404, 440
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 8 cut(s) 227, 252, 758, 761, 773, 788, 797, 843
BstNI CCWGG 2 cut(s) 602, 784
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 156, 600, 782
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 9 cut(s) 641, 644, 736, 748, 751, 763, 764, 766, 849
BstXI CCANNNNNNTGG 1 cut(s) 614
BsuRI GGCC 5 cut(s) 155, 185, 251, 456, 477
BtsCI GGATG 2 cut(s) 208, 751
BtsIMutI CAGTG 3 cut(s) 334, 457, 645
Cac8I GCNNGC 3 cut(s) 253, 798, 855
CciI TCATGA 1 cut(s) 376
CfoI GCGC 2 cut(s) 67, 232
Cfr10I RCCGGY 2 cut(s) 251, 840
Cfr13I GGNCC 7 cut(s) 153, 154, 183, 249, 268, 476, 604
CviAII CATG 3 cut(s) 180, 299, 377
DdeI CTNAG 4 cut(s) 294, 408, 423, 654
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 2 cut(s) 110, 327
EarI CTCTTC 2 cut(s) 110, 327
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 2 cut(s) 347, 440
Eco47I GGWCC 2 cut(s) 268, 604
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 600, 782
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 3 cut(s) 183, 302, 380
FaiI YATR 6 cut(s) 181, 300, 378, 515, 716, 860
FatI CATG 3 cut(s) 179, 298, 376
FauI CCCGC 1 cut(s) 5
FokI GGATG 2 cut(s) 215, 758
FriOI GRGCYC 1 cut(s) 157
FspBI CTAG 2 cut(s) 428, 792
GlaI GCGC 2 cut(s) 66, 231
GsaI CCCAGC 2 cut(s) 71, 250
GsuI CTGGAG 2 cut(s) 308, 489
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 5 cut(s) 155, 185, 251, 456, 477
HapII CCGG 5 cut(s) 134, 158, 236, 252, 841
HhaI GCGC 2 cut(s) 67, 232
Hin1II CATG 3 cut(s) 183, 302, 380
Hin6I GCGC 2 cut(s) 65, 230
HinP1I GCGC 2 cut(s) 65, 230
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 6 cut(s) 29, 260, 464, 587, 596, 822
HpaII CCGG 5 cut(s) 134, 158, 236, 252, 841
HphI GGTGA 4 cut(s) 269, 323, 521, 692
Hpy166II GTNNAC 2 cut(s) 367, 675
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 4 cut(s) 344, 377, 468, 591
Hpy8I GTNNAC 2 cut(s) 367, 675
HpyAV CCTTC 7 cut(s) 16, 133, 332, 517, 562, 577, 652
HpyCH4III ACNGT 3 cut(s) 329, 415, 866
HpyCH4IV ACGT 3 cut(s) 54, 171, 671
HpyCH4V TGCA 3 cut(s) 629, 752, 767
HpyF10VI GCNNNNNNNGC 8 cut(s) 227, 252, 758, 761, 773, 788, 797, 843
HpyF3I CTNAG 4 cut(s) 294, 408, 423, 654
HpySE526I ACGT 3 cut(s) 54, 171, 671
Hsp92II CATG 3 cut(s) 183, 302, 380
HspAI GCGC 2 cut(s) 65, 230
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 15, 72, 130, 289
Lsp1109I GCAGC 9 cut(s) 641, 644, 736, 748, 751, 763, 764, 766, 849
LweI GCATC 2 cut(s) 719, 736
MaeI CTAG 2 cut(s) 428, 792
MaeII ACGT 3 cut(s) 54, 171, 671
MaeIII GTNAC 3 cut(s) 257, 323, 509
MalI GATC 1 cut(s) 375
MbiI CCGCTC 3 cut(s) 120, 166, 694
MboI GATC 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 157
MlsI TGGCCA 1 cut(s) 456
MluCI AATT 2 cut(s) 239, 491
MluNI TGGCCA 1 cut(s) 456
MlyI GAGTC 3 cut(s) 38, 254, 596
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 6 cut(s) 136, 193, 298, 328, 364, 541
Mox20I TGGCCA 1 cut(s) 456
MroNI GCCGGC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 456
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 2 cut(s) 53, 381
Msp20I TGGCCA 1 cut(s) 456
MspI CCGG 5 cut(s) 134, 158, 236, 252, 841
MspR9I CCNGG 3 cut(s) 158, 602, 784
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 2 cut(s) 602, 784
MwoI GCNNNNNNNGC 8 cut(s) 227, 252, 758, 761, 773, 788, 797, 843
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 1 cut(s) 158
NdeII GATC 1 cut(s) 373
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 183, 302, 380
NlaIV GGNNCC 6 cut(s) 132, 155, 156, 162, 291, 478
NmuCI GTSAC 3 cut(s) 257, 323, 509
NspI RCATGY 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 53
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PctI GAATGC 1 cut(s) 793
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 464, 596, 822
PleI GAGTC 3 cut(s) 37, 254, 595
PpsI GAGTC 3 cut(s) 37, 254, 595
Psp6I CCWGG 2 cut(s) 600, 782
PspFI CCCAGC 2 cut(s) 67, 246
PspGI CCWGG 2 cut(s) 600, 782
PspN4I GGNNCC 6 cut(s) 132, 155, 156, 162, 291, 478
PspOMI GGGCCC 1 cut(s) 153
PspPI GGNCC 7 cut(s) 153, 154, 183, 249, 268, 476, 604
PstI CTGCAG 1 cut(s) 754
RseI CAYNNNNRTG 2 cut(s) 53, 381
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 687
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 7 cut(s) 153, 154, 183, 249, 268, 476, 604
SchI GAGTC 3 cut(s) 38, 254, 596
ScrFI CCNGG 3 cut(s) 158, 602, 784
SduI GDGCHC 1 cut(s) 157
SfaNI GCATC 2 cut(s) 719, 736
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 2 cut(s) 268, 604
SmiMI CAYNNNNRTG 2 cut(s) 53, 381
Sse9I AATT 2 cut(s) 239, 491
SsiI CCGC 9 cut(s) 5, 12, 62, 120, 128, 164, 233, 255, 694
SspMI CTAG 2 cut(s) 428, 792
StyD4I CCNGG 3 cut(s) 156, 600, 782
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 3 cut(s) 329, 415, 866
TaiI ACGT 3 cut(s) 57, 174, 674
TaqI TCGA 4 cut(s) 192, 345, 581, 590
TasI AATT 2 cut(s) 239, 491
TauI GCSGC 3 cut(s) 65, 235, 697
TfiI GAWTC 3 cut(s) 464, 596, 822
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 3 cut(s) 334, 457, 652
TseFI GTSAC 3 cut(s) 257, 323, 509
TseI GCWGC 9 cut(s) 629, 632, 749, 752, 761, 764, 776, 779, 837
Tsp45I GTSAC 3 cut(s) 257, 323, 509
TspDTI ATGAA 2 cut(s) 399, 731
TspRI CASTG 3 cut(s) 334, 457, 652
VpaK11BI GGWCC 2 cut(s) 268, 604
XceI RCATGY 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XspI CTAG 2 cut(s) 428, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.