MD15G1190400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014631
Physical Location & Seq
Forward (+)
14990225 .. 14993676
3452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1190400.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCGGGAGGCGCGAGCAGGAAGGACGAGTCGGTGGTCCTGAACAGCACCAATGTTTTTGCGGCTCTTGGGAGCTTGAAGAAGAAGAAGAAGGGGGACAAGAGCTCGTCCAAAAGCTCGAAAGGCGGCGGCGCAGCTCAGGATTCTGGGGAGGCGGAGAAGGAGGTCTTTTGGGCGCCGGCGCCCCTTATCGCGAAGTCGTGGGCCGATGTCGATGACGAGGACGATGACGACTACTATGCAACTACAGCTCCGCCGGAAATGGGTTGGGCCGGTGAGGATTCGAAGGCGGCGAAGGAGAGCGAGCTGGAGGCTGAGCATGCGGAGGCCGGCGAATTGGAAAGTGAAAGTGAGGAGGAAGGTGGTGATGAAGTTGATGATGTTGATGAGGAACATGAAAATGAACTTGAAGCCCCATTGGAAACTGAGCCAGTTAAGAAGCTCCCTGAACCTTCTCCAGCCCCCAAGGATACAGAAAGGCAGCTTTCTAAGAAGGAACTGAAGAAGAAGGGGCTTGAAGAACTTGAAGCAGTTCTTGCTGAGCTAGGATACCCTACCAACACCGAGACTAGTGGCCAAGATGATTCCAGTGGTGTTACCCAAGAGAAGAAAGTAGAGAATCAAAATGGCGATGTGGACAAGAAGGAGAATGCCACTGGCGAGAGCAAAAGTGCTAAAAAGAAGAAAAAGAAGGACAAGTCATCGAAGGAGCCAAAAGAATCCCAAGACCAGCCCGATGGCACAAATGGTGGAAGTACAGCAGCAGATGAAAACACTGGGACGGATAAGGGAGAAGATGCATCTTCTGGTGATGTTAAAGAGCGGCTAAAGAAAGTGGCGTCCATGAAGAAGAAGAAGTCAAGCAAAGAAATGGATGCAGCCGCTCGAGCTGCTGCAAGTGAAGCTGCTGCCAGGAACGCTAGGCTAGCTGCCTCGAAGAAAAAAGAGAAGAATCACTACAATCAGCAGCCAGTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

34.9

Weight (kDa)

4.88

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014631)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05450
malus_domestica MD02G1062600.v1.1 MD15G1190400.v1.1
prunus_persica Prupe.7G226700_v2.0.a1
pyrus_communis pycom02g04330 pycom15g17060
rosa_chinensis RchiOBHm_Chr2g0091121
rosa_laevigata RLG00000016181
rosa_multiflora Rmu_sc0004851.1_g000008
rosa_roxburghii Rroxscaffold_2G00150530
rosa_rugosa Rorug02G0013100 Rorug02G0013200
rosa_samantha Rh2AG059400 Rh2BG057800 Rh2CG059600 Rh2DG058700
rosa_wichuraiana Rw2G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 175, 181
AccBSI CCGCTC 2 cut(s) 823, 884
AccII CGCG 2 cut(s) 14, 194
AcoI YGGCCR 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 521
AcyI GRCGYC 3 cut(s) 176, 182, 839
AfaI GTAC 1 cut(s) 757
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 4 cut(s) 79, 410, 518, 527
AhlI ACTAGT 1 cut(s) 569
AjnI CCWGG 1 cut(s) 911
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 885
AoxI GGCC 4 cut(s) 204, 270, 327, 574
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 4 cut(s) 14, 134, 178, 184
AspS9I GGNCC 3 cut(s) 37, 204, 270
AsuHPI GGTGA 3 cut(s) 287, 377, 821
AsuII TTCGAA 1 cut(s) 284
AsuNHI GCTAGC 1 cut(s) 925
AvaI CYCGRG 1 cut(s) 885
AvaII GGWCC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 576
BanI GGYRCC 2 cut(s) 175, 181
BanII GRGCYC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 9 cut(s) 146, 493, 773, 877, 880, 890, 892, 895, 916
BccI CCATC 1 cut(s) 731
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 1 cut(s) 913
BciVI GTATCC 2 cut(s) 463, 542
BcoDI GTCTC 1 cut(s) 560
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 4 cut(s) 545, 570, 921, 926
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 2 cut(s) 179, 185
BfuI GTATCC 2 cut(s) 463, 542
BlpI GCTNAGC 2 cut(s) 315, 540
Bme1390I CCNGG 1 cut(s) 913
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 885
BmgT120I GGNCC 3 cut(s) 37, 204, 270
BmiI GGNNCC 3 cut(s) 177, 183, 711
BmrFI CCNGG 1 cut(s) 913
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 3 cut(s) 787, 809, 865
BmtI GCTAGC 1 cut(s) 929
BmuI ACTGGG 1 cut(s) 786
BpmI CTGGAG 2 cut(s) 329, 441
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 2 cut(s) 315, 540
Bpu14I TTCGAA 1 cut(s) 284
BsaHI GRCGYC 3 cut(s) 176, 182, 839
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse118I RCCGGY 3 cut(s) 178, 272, 329
Bse1I ACTGG 5 cut(s) 431, 588, 661, 781, 971
BseBI CCWGG 1 cut(s) 913
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 880
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMII CTCAG 4 cut(s) 152, 306, 417, 531
BseNI ACTGG 5 cut(s) 431, 588, 661, 781, 971
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 9 cut(s) 146, 493, 773, 877, 880, 890, 892, 895, 916
Bsh1236I CGCG 2 cut(s) 14, 194
BshFI GGCC 4 cut(s) 206, 272, 329, 576
BshNI GGYRCC 2 cut(s) 175, 181
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 4 cut(s) 179, 257, 273, 330
BslFI GGGAC 2 cut(s) 110, 793
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 110, 793
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 4 cut(s) 206, 272, 329, 576
BsoBI CYCGRG 1 cut(s) 885
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 107
Bsp1720I GCTNAGC 2 cut(s) 315, 540
Bsp68I TCGCGA 1 cut(s) 194
BspANI GGCC 4 cut(s) 206, 272, 329, 576
BspCNI CTCAG 4 cut(s) 151, 307, 418, 532
BspFNI CGCG 2 cut(s) 14, 194
BspLI GGNNCC 3 cut(s) 177, 183, 711
BspOI GCTAGC 1 cut(s) 929
BspT104I TTCGAA 1 cut(s) 284
BspT107I GGYRCC 2 cut(s) 175, 181
BsrBI CCGCTC 2 cut(s) 823, 884
BsrFI RCCGGY 3 cut(s) 178, 272, 329
BsrI ACTGG 5 cut(s) 431, 588, 661, 781, 971
BssAI RCCGGY 3 cut(s) 178, 272, 329
BssECI CCNNGG 1 cut(s) 465
BssNI GRCGYC 3 cut(s) 176, 182, 839
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 913
BstACI GRCGYC 3 cut(s) 176, 182, 839
BstAPI GCANNNNNTGC 2 cut(s) 536, 973
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 6 cut(s) 16, 180, 305, 321, 331, 927
BstDEI CTNAG 5 cut(s) 138, 315, 426, 489, 540
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 2 cut(s) 14, 194
BstH2I RGCGCY 2 cut(s) 179, 185
BstHHI GCGC 4 cut(s) 14, 134, 178, 184
BstMAI GTCTC 1 cut(s) 560
BstNI CCWGG 1 cut(s) 913
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 911
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 2 cut(s) 14, 194
BstV1I GCAGC 9 cut(s) 146, 493, 773, 877, 880, 890, 892, 895, 916
BstXI CCANNNNNNTGG 1 cut(s) 737
BsuI GTATCC 2 cut(s) 463, 542
BsuRI GGCC 4 cut(s) 206, 272, 329, 576
BtgZI GCGATG 1 cut(s) 645
BtsCI GGATG 1 cut(s) 880
BtsIMutI CAGTG 4 cut(s) 595, 654, 774, 978
BtuMI TCGCGA 1 cut(s) 194
Cac8I GCNNGC 6 cut(s) 16, 180, 305, 321, 331, 927
CfoI GCGC 4 cut(s) 14, 134, 178, 184
Cfr10I RCCGGY 3 cut(s) 178, 272, 329
Cfr13I GGNCC 3 cut(s) 37, 204, 270
CseI GACGC 1 cut(s) 828
Csp6I GTAC 1 cut(s) 756
CviAII CATG 3 cut(s) 320, 395, 844
CviQI GTAC 1 cut(s) 756
DdeI CTNAG 5 cut(s) 138, 315, 426, 489, 540
DinI GGCGCC 2 cut(s) 177, 183
EaeI YGGCCR 1 cut(s) 574
EciI GGCGGA 2 cut(s) 170, 243
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 885
EcoICRI GAGCTC 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 911
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 107
EgeI GGCGCC 2 cut(s) 177, 183
EheI GGCGCC 2 cut(s) 177, 183
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 3 cut(s) 323, 398, 847
FaiI YATR 4 cut(s) 240, 321, 396, 845
FalI AAGNNNNNCTT 4 cut(s) 152, 184, 519, 551
FaqI GGGAC 2 cut(s) 110, 793
FatI CATG 3 cut(s) 319, 394, 843
FokI GGATG 1 cut(s) 887
FriOI GRGCYC 1 cut(s) 107
FspBI CTAG 4 cut(s) 545, 570, 921, 926
GlaI GCGC 4 cut(s) 13, 133, 177, 183
GsuI CTGGAG 2 cut(s) 329, 441
HaeII RGCGCY 2 cut(s) 179, 185
HaeIII GGCC 4 cut(s) 206, 272, 329, 576
HapII CCGG 4 cut(s) 179, 257, 273, 330
HgaI GACGC 1 cut(s) 828
HhaI GCGC 4 cut(s) 14, 134, 178, 184
Hin1I GRCGYC 3 cut(s) 176, 182, 839
Hin1II CATG 3 cut(s) 323, 398, 847
Hin6I GCGC 4 cut(s) 12, 132, 176, 182
HinP1I GCGC 4 cut(s) 12, 132, 176, 182
HinfI GANTC 7 cut(s) 29, 143, 281, 584, 619, 719, 952
HpaII CCGG 4 cut(s) 179, 257, 273, 330
HphI GGTGA 3 cut(s) 287, 377, 821
Hpy166II GTNNAC 1 cut(s) 637
Hpy188III TCNNGA 3 cut(s) 40, 140, 193
Hpy8I GTNNAC 1 cut(s) 637
HpyCH4V TGCA 4 cut(s) 242, 800, 878, 896
HpyF3I CTNAG 5 cut(s) 138, 315, 426, 489, 540
Hsp92I GRCGYC 3 cut(s) 176, 182, 839
Hsp92II CATG 3 cut(s) 323, 398, 847
HspAI GCGC 4 cut(s) 12, 132, 176, 182
KasI GGCGCC 2 cut(s) 175, 181
KroI GCCGGC 2 cut(s) 178, 329
KroNI GCCGGC 2 cut(s) 180, 331
LmnI GCTCC 4 cut(s) 72, 256, 447, 709
Lsp1109I GCAGC 9 cut(s) 146, 493, 773, 877, 880, 890, 892, 895, 916
LweI GCATC 3 cut(s) 787, 809, 865
MaeI CTAG 4 cut(s) 545, 570, 921, 926
MaeIII GTNAC 1 cut(s) 595
MbiI CCGCTC 2 cut(s) 823, 884
MhlI GDGCHC 1 cut(s) 107
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 1 cut(s) 335
MluNI TGGCCA 1 cut(s) 576
Mly113I GGCGCC 2 cut(s) 176, 182
MlyI GAGTC 1 cut(s) 38
Mox20I TGGCCA 1 cut(s) 576
Mph1103I ATGCAT 1 cut(s) 802
MreI CGCCGGCG 1 cut(s) 178
MroNI GCCGGC 2 cut(s) 178, 329
MroXI GAANNNNTTC 1 cut(s) 531
MscI TGGCCA 1 cut(s) 576
MseI TTAA 2 cut(s) 435, 816
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 576
MspI CCGG 4 cut(s) 179, 257, 273, 330
MspR9I CCNGG 1 cut(s) 913
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 1 cut(s) 913
MvnI CGCG 2 cut(s) 14, 194
NaeI GCCGGC 2 cut(s) 180, 331
NarI GGCGCC 2 cut(s) 176, 182
NgoMIV GCCGGC 2 cut(s) 178, 329
NheI GCTAGC 1 cut(s) 925
NlaIII CATG 3 cut(s) 323, 398, 847
NlaIV GGNNCC 3 cut(s) 177, 183, 711
NruI TCGCGA 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 1 cut(s) 323
NspV TTCGAA 1 cut(s) 284
PaeI GCATGC 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PctI GAATGC 1 cut(s) 655
PdiI GCCGGC 2 cut(s) 180, 331
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 6 cut(s) 143, 281, 584, 619, 719, 952
PleI GAGTC 1 cut(s) 37
PluTI GGCGCC 2 cut(s) 179, 185
PpsI GAGTC 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 911
PspGI CCWGG 1 cut(s) 911
PspN4I GGNNCC 3 cut(s) 177, 183, 711
PspPI GGNCC 3 cut(s) 37, 204, 270
PspXI VCTCGAGB 1 cut(s) 885
RruI TCGCGA 1 cut(s) 194
RsaI GTAC 1 cut(s) 757
RsaNI GTAC 1 cut(s) 756
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 435, 816
Sau96I GGNCC 3 cut(s) 37, 204, 270
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 913
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 3 cut(s) 787, 809, 865
SfcI CTRYAG 1 cut(s) 246
SfoI GGCGCC 2 cut(s) 177, 183
Sfr274I CTCGAG 1 cut(s) 885
SfuI TTCGAA 1 cut(s) 284
SgrAI CRCCGGYG 1 cut(s) 178
SinI GGWCC 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
SpeI ACTAGT 1 cut(s) 569
SphI GCATGC 1 cut(s) 323
Sse9I AATT 1 cut(s) 335
SspDI GGCGCC 2 cut(s) 175, 181
SspMI CTAG 4 cut(s) 545, 570, 921, 926
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 911
StyI CCWWGG 1 cut(s) 465
TaqI TCGA 6 cut(s) 119, 213, 284, 704, 886, 935
TasI AATT 1 cut(s) 335
TatI WGTACW 1 cut(s) 755
TauI GCSGC 6 cut(s) 65, 129, 132, 293, 826, 884
TfiI GAWTC 6 cut(s) 143, 281, 584, 619, 719, 952
Tru1I TTAA 2 cut(s) 435, 816
Tru9I TTAA 2 cut(s) 435, 816
TscAI CASTG 4 cut(s) 595, 661, 781, 978
TspDTI ATGAA 5 cut(s) 384, 411, 417, 783, 860
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 4 cut(s) 595, 661, 781, 978
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 885
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 4 cut(s) 545, 570, 921, 926
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.