Rw2G005040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014631
Physical Location & Seq
Reverse (-)
4732113 .. 4735923
3811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005040.1

Sequence Viewer

Length: 951 bp
ATGGCGGGAGGCGGGAGCAGGAAGGACGAGTCGGTGGTGCTGAACAGCACGAACGTGTTTGCGGCGCTGGGGAGCTTGAAGAAGAAGAAGAAGTCCGATAAGTCAAAGAGTGGGAAGAGCGGTCAGGCCGAGCCGGAGAAGGAGGTTTATTGGGCCCCGGCGCCGCTCACCGTCAAGTCATGGGCCGATGTCGATGACGAGGATGATGACGACTACTTTGCGACTACTGCGCCGCCGGAATTGGGCTGGGCCGGCGGTGACTCCAAGGACCACAAGGAGAGTGAGCTGGAGCCTGAGCATGTGGAGGTCGGTGATTTGGTAAGTGACAGTGAAGAGGAAGGTCTCGATGATGTTGATGATGATGTGGACGAGGATCATGATAACGAACTTGAAGCACCAGTTGAAACTGGGACAGGTAAGAAGCTAGCTGAAGCTTCTACAGCTCCTAAGGATACAGAAAGGCAGCTTTCTAAGAAGGAACTGAAGAAAAAAGGACTTGAAGAATTGGATGCAGTTCTTGCTGAGCTAGGACTTGGCAAAAATGAGACCAGTGGCCACATTGAATCTGGAGATGGCGCCCAAGAGAAGAAATTAGAGAACCTAAATGGTGACATAGACAAGAAGGAAACCTCTGGGGAGAGCAAAAGTGCCAAAAAGAAGAAAAAGAAGGATAAGTCATCAAAGGAGTCGAGAGAATCTCAGGACCAGCCTGATGGCGTTGGAAATGCAGCAGCAGAAGAAACCACTGAAACCGAGAAGGTAGAAGATACGTCTACTGGTGATGTTAAAGAGCGGCTAAAGAAAGTGGCATCTATGAAGAAGAAGAAATCAAGCAAGGAAATGGATGCTGCAGCCCGAGCTGCTGCAAGTGAAGCTGCTGCCAGGAATGCTAGGCTAGCTTCAGCAAAGAAAAAAGAGAAGAATCACTACAATCAGCAGCCGGTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

34.13

Weight (kDa)

4.99

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014631)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05450
malus_domestica MD02G1062600.v1.1 MD15G1190400.v1.1
prunus_persica Prupe.7G226700_v2.0.a1
pyrus_communis pycom02g04330 pycom15g17060
rosa_chinensis RchiOBHm_Chr2g0091121
rosa_laevigata RLG00000016181
rosa_multiflora Rmu_sc0004851.1_g000008
rosa_roxburghii Rroxscaffold_2G00150530
rosa_rugosa Rorug02G0013100 Rorug02G0013200
rosa_samantha Rh2AG059400 Rh2BG057800 Rh2CG059600 Rh2DG058700
rosa_wichuraiana Rw2G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 160, 575
AccBSI CCGCTC 3 cut(s) 120, 166, 793
AccI GTMKAC 1 cut(s) 773
AciI CCGC 8 cut(s) 5, 12, 62, 120, 164, 233, 255, 793
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 553
AcuI CTGAAG 3 cut(s) 450, 503, 885
AcyI GRCGYC 2 cut(s) 161, 576
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 5 cut(s) 79, 392, 404, 500, 563
AjnI CCWGG 1 cut(s) 881
AleI CACNNNNGTG 1 cut(s) 53
Alw26I GTCTC 2 cut(s) 347, 539
AlwI GGATC 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 855
AoxI GGCC 5 cut(s) 126, 153, 183, 249, 553
ApaI GGGCCC 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 200, 232
AspLEI GCGC 4 cut(s) 67, 163, 232, 578
AspS9I GGNCC 6 cut(s) 153, 154, 183, 249, 268, 703
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 5 cut(s) 160, 269, 323, 620, 791
AsuNHI GCTAGC 2 cut(s) 424, 895
AvaI CYCGRG 1 cut(s) 855
AvaII GGWCC 2 cut(s) 268, 703
AxyI CCTNAGG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 555
BanI GGYRCC 2 cut(s) 160, 575
BanII GRGCYC 1 cut(s) 157
BarI GAAGNNNNNNTAC 2 cut(s) 911, 943
BbvI GCAGC 9 cut(s) 475, 740, 743, 835, 847, 850, 862, 863, 865
BccI CCATC 2 cut(s) 566, 707
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 1 cut(s) 883
BciVI GTATCC 1 cut(s) 445
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 2 cut(s) 347, 539
BfaI CTAG 4 cut(s) 425, 527, 891, 896
BfmI CTRYAG 2 cut(s) 438, 849
BfoI RGCGCY 3 cut(s) 68, 164, 579
BfuI GTATCC 1 cut(s) 445
BlpI GCTNAGC 1 cut(s) 522
Bme1390I CCNGG 2 cut(s) 158, 883
Bme18I GGWCC 2 cut(s) 268, 703
BmeT110I CYCGRG 1 cut(s) 855
BmgT120I GGNCC 6 cut(s) 153, 154, 183, 249, 268, 703
BmiI GGNNCC 5 cut(s) 155, 156, 162, 291, 577
BmrFI CCNGG 2 cut(s) 158, 883
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 3 cut(s) 499, 818, 835
BmtI GCTAGC 2 cut(s) 428, 899
BmuI ACTGGG 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 682, 714
BpmI CTGGAG 2 cut(s) 308, 588
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 161, 576
BsaI GGTCTC 2 cut(s) 347, 539
BsaJI CCNNGG 2 cut(s) 156, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse118I RCCGGY 2 cut(s) 251, 940
Bse1I ACTGG 4 cut(s) 398, 412, 549, 781
Bse21I CCTNAGG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 2 cut(s) 156, 264
BseGI GGATG 3 cut(s) 208, 514, 850
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 3 cut(s) 285, 513, 713
BseNI ACTGG 4 cut(s) 398, 412, 549, 781
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 9 cut(s) 475, 740, 743, 835, 847, 850, 862, 863, 865
BseYI CCCAGC 2 cut(s) 67, 246
BshFI GGCC 5 cut(s) 128, 155, 185, 251, 555
BshNI GGYRCC 2 cut(s) 160, 575
BsiHKCI CYCGRG 1 cut(s) 855
BsiSI CCGG 5 cut(s) 134, 158, 236, 252, 941
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 347, 539
BsmFI GGGAC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 5 cut(s) 128, 155, 185, 251, 555
Bso31I GGTCTC 2 cut(s) 347, 539
BsoBI CYCGRG 1 cut(s) 855
Bsp120I GGGCCC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 1 cut(s) 373
Bsp1720I GCTNAGC 1 cut(s) 522
BspACI CCGC 8 cut(s) 5, 12, 62, 120, 164, 233, 255, 793
BspANI GGCC 5 cut(s) 128, 155, 185, 251, 555
BspCNI CTCAG 3 cut(s) 286, 514, 712
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 5 cut(s) 155, 156, 162, 291, 577
BspMAI CTGCAG 1 cut(s) 853
BspOI GCTAGC 2 cut(s) 428, 899
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 2 cut(s) 160, 575
BspTNI GGTCTC 2 cut(s) 347, 539
BsrBI CCGCTC 3 cut(s) 120, 166, 793
BsrFI RCCGGY 2 cut(s) 251, 940
BsrI ACTGG 4 cut(s) 398, 412, 549, 781
BssAI RCCGGY 2 cut(s) 251, 940
BssECI CCNNGG 2 cut(s) 156, 264
BssMI GATC 1 cut(s) 373
BssNI GRCGYC 2 cut(s) 161, 576
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 2 cut(s) 172, 329
Bst6I CTCTTC 2 cut(s) 110, 327
BstACI GRCGYC 2 cut(s) 161, 576
BstAPI GCANNNNNTGC 2 cut(s) 518, 943
BstC8I GCNNGC 3 cut(s) 253, 426, 897
BstDEI CTNAG 5 cut(s) 294, 447, 471, 522, 699
BstF5I GGATG 3 cut(s) 208, 514, 850
BstH2I RGCGCY 3 cut(s) 68, 164, 579
BstHHI GCGC 4 cut(s) 67, 163, 232, 578
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 2 cut(s) 347, 539
BstMBI GATC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 156, 881
BstSFI CTRYAG 2 cut(s) 438, 849
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 9 cut(s) 475, 740, 743, 835, 847, 850, 862, 863, 865
BstXI CCANNNNNNTGG 1 cut(s) 713
Bsu36I CCTNAGG 1 cut(s) 447
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 5 cut(s) 128, 155, 185, 251, 555
BtsCI GGATG 3 cut(s) 208, 514, 850
BtsIMutI CAGTG 3 cut(s) 334, 556, 744
Cac8I GCNNGC 3 cut(s) 253, 426, 897
CciI TCATGA 1 cut(s) 376
CfoI GCGC 4 cut(s) 67, 163, 232, 578
Cfr10I RCCGGY 2 cut(s) 251, 940
Cfr13I GGNCC 6 cut(s) 153, 154, 183, 249, 268, 703
CviAII CATG 3 cut(s) 180, 299, 377
DdeI CTNAG 5 cut(s) 294, 447, 471, 522, 699
DinI GGCGCC 2 cut(s) 162, 577
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 2 cut(s) 110, 327
EarI CTCTTC 2 cut(s) 110, 327
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 2 cut(s) 347, 539
Eco47I GGWCC 2 cut(s) 268, 703
Eco57I CTGAAG 3 cut(s) 450, 503, 885
Eco81I CCTNAGG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 855
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 881
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 157
EgeI GGCGCC 2 cut(s) 162, 577
EheI GGCGCC 2 cut(s) 162, 577
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 3 cut(s) 183, 302, 380
FaiI YATR 5 cut(s) 181, 300, 378, 614, 815
FaqI GGGAC 1 cut(s) 424
FatI CATG 3 cut(s) 179, 298, 376
FauI CCCGC 1 cut(s) 5
FblI GTMKAC 1 cut(s) 773
FokI GGATG 3 cut(s) 215, 521, 857
FriOI GRGCYC 1 cut(s) 157
FspBI CTAG 4 cut(s) 425, 527, 891, 896
GlaI GCGC 4 cut(s) 66, 162, 231, 577
GsaI CCCAGC 2 cut(s) 71, 250
GsuI CTGGAG 2 cut(s) 308, 588
HaeII RGCGCY 3 cut(s) 68, 164, 579
HaeIII GGCC 5 cut(s) 128, 155, 185, 251, 555
HapII CCGG 5 cut(s) 134, 158, 236, 252, 941
HhaI GCGC 4 cut(s) 67, 163, 232, 578
Hin1I GRCGYC 2 cut(s) 161, 576
Hin1II CATG 3 cut(s) 183, 302, 380
Hin6I GCGC 4 cut(s) 65, 161, 230, 576
HinP1I GCGC 4 cut(s) 65, 161, 230, 576
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 6 cut(s) 29, 260, 563, 686, 695, 922
HpaII CCGG 5 cut(s) 134, 158, 236, 252, 941
HphI GGTGA 5 cut(s) 160, 269, 323, 620, 791
Hpy166II GTNNAC 2 cut(s) 367, 774
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 5 cut(s) 344, 377, 567, 690, 701
Hpy8I GTNNAC 2 cut(s) 367, 774
HpyAV CCTTC 7 cut(s) 16, 133, 332, 469, 616, 661, 751
HpyCH4III ACNGT 2 cut(s) 172, 329
HpyCH4IV ACGT 2 cut(s) 54, 770
HpyCH4V TGCA 4 cut(s) 512, 728, 851, 866
HpyF3I CTNAG 5 cut(s) 294, 447, 471, 522, 699
HpySE526I ACGT 2 cut(s) 54, 770
Hsp92I GRCGYC 2 cut(s) 161, 576
Hsp92II CATG 3 cut(s) 183, 302, 380
HspAI GCGC 4 cut(s) 65, 161, 230, 576
KasI GGCGCC 2 cut(s) 160, 575
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 15, 72, 289, 448
Lsp1109I GCAGC 9 cut(s) 475, 740, 743, 835, 847, 850, 862, 863, 865
LweI GCATC 3 cut(s) 499, 818, 835
MaeI CTAG 4 cut(s) 425, 527, 891, 896
MaeII ACGT 2 cut(s) 54, 770
MaeIII GTNAC 3 cut(s) 257, 323, 608
MalI GATC 1 cut(s) 375
MbiI CCGCTC 3 cut(s) 120, 166, 793
MboI GATC 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 157
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 3 cut(s) 239, 503, 590
MluNI TGGCCA 1 cut(s) 555
Mly113I GGCGCC 2 cut(s) 161, 576
MlyI GAGTC 3 cut(s) 38, 254, 695
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 6 cut(s) 136, 193, 298, 328, 364, 640
Mox20I TGGCCA 1 cut(s) 555
MroNI GCCGGC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 555
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 555
MspI CCGG 5 cut(s) 134, 158, 236, 252, 941
MspR9I CCNGG 2 cut(s) 158, 883
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 1 cut(s) 883
NaeI GCCGGC 1 cut(s) 253
NarI GGCGCC 2 cut(s) 161, 576
NciI CCSGG 1 cut(s) 158
NdeII GATC 1 cut(s) 373
NgoMIV GCCGGC 1 cut(s) 251
NheI GCTAGC 2 cut(s) 424, 895
NlaIII CATG 3 cut(s) 183, 302, 380
NlaIV GGNNCC 5 cut(s) 155, 156, 162, 291, 577
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 3 cut(s) 257, 323, 608
NspI RCATGY 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 53
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 110
PctI GAATGC 1 cut(s) 892
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 563, 695, 922
PleI GAGTC 3 cut(s) 37, 254, 694
PluTI GGCGCC 2 cut(s) 164, 579
PpsI GAGTC 3 cut(s) 37, 254, 694
Psp6I CCWGG 1 cut(s) 881
PspFI CCCAGC 2 cut(s) 67, 246
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 5 cut(s) 155, 156, 162, 291, 577
PspOMI GGGCCC 1 cut(s) 153
PspPI GGNCC 6 cut(s) 153, 154, 183, 249, 268, 703
PstI CTGCAG 1 cut(s) 853
RseI CAYNNNNRTG 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 786
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 6 cut(s) 153, 154, 183, 249, 268, 703
SchI GAGTC 3 cut(s) 38, 254, 695
ScrFI CCNGG 2 cut(s) 158, 883
SduI GDGCHC 1 cut(s) 157
SfaNI GCATC 3 cut(s) 499, 818, 835
SfcI CTRYAG 2 cut(s) 438, 849
SfoI GGCGCC 2 cut(s) 162, 577
SinI GGWCC 2 cut(s) 268, 703
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 3 cut(s) 239, 503, 590
SsiI CCGC 8 cut(s) 5, 12, 62, 120, 164, 233, 255, 793
SspDI GGCGCC 2 cut(s) 160, 575
SspMI CTAG 4 cut(s) 425, 527, 891, 896
StyD4I CCNGG 2 cut(s) 156, 881
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 172, 329
TaiI ACGT 2 cut(s) 57, 773
TaqI TCGA 3 cut(s) 192, 345, 689
TasI AATT 3 cut(s) 239, 503, 590
TauI GCSGC 4 cut(s) 65, 166, 235, 796
TfiI GAWTC 3 cut(s) 563, 695, 922
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 3 cut(s) 334, 556, 751
TseFI GTSAC 3 cut(s) 257, 323, 608
Tsp45I GTSAC 3 cut(s) 257, 323, 608
TspDTI ATGAA 1 cut(s) 830
TspRI CASTG 3 cut(s) 334, 556, 751
VpaK11BI GGWCC 2 cut(s) 268, 703
XceI RCATGY 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 773
XspI CTAG 4 cut(s) 425, 527, 891, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.