Rorug02G0013100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014631
Physical Location & Seq
Reverse (-)
1116988 .. 1118121
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0013100.1

Sequence Viewer

Length: 1134 bp
ATGTACACCAGACTGATGATGAATTATGAGGACGCTCCAATAGAAGATCGCAATCGTCACGAGGTCAAAGACCTCTCTATAGAGGAAGTACAGGAGAAACTTGAAGCTCTCAAGAAAAAATTAGATGATGGGAGCTTTAGTTGGATTTGTCTAGACAAGTCCAACGTGACCTATGGTGAGTGTAGCGATGGCGTCAAACGTCTTACAGTGAAGGATATAAATAAACGCTCTAAACCAAGAGCTGAAAAAAGAGAAAAGATCCGTTCGATGGTTGATCTTATTCTCGGGTTCTTCCAAGCAAACAAGCGAGAAAGACATCGTGGGGTGTTTTACGTAGGGAATAATACCACCAGTTCATTGTTCACACAAGACGAGGAGTACGCAATTCTTAATGACATCTGTTGCACTATAGGCTGCAGCAGTTCAAGTCTCTACTACATAGATGCTAGTGACGTAAGAGGCGTTGTTGGAGGTCTGCTTTCGTGGGTGCAGAATGGGTACGAAACCGATTGTCGAAGCCGGGATGATGGGGTGGCCATCCCGTCCATGCATTCAGATATTAGTTTGAAAAATTGTGGAGCCAAGTTCGTATTGGTGGTGGAAAACAAAGCCAGTTTTTATAATCTCATGCAAGACAGGTTTTACCGTGATTATCCATGCATAATCATCACCGGAATGGGAAAGCCCGGGGTGGCGACCAGAAGATTTTTAAAGCTATTGTCGGACAATTTTAGGCTTCCAGTGTATGGCCTTTTCGACTGTGATCCAGCAAGCATCAAAATGTTTTCGATTTATACTGTTGGGTCATATGAGGAGGCCTTCGATAGTGTAAACTTAACTTGCCCGTATATGACATGGTTGGGTGTTTGGCCGAGTGACCTATGTGCGCTTGACATACCCTGCGACGATATGAGTCCAAAAGAGATTAGAGACATGGATAATCTTTTGAAAGAAGATTTTGTGAAAGATAATCCGAGGTTAGTTGAGGAGCTGGAACTTATGAAAGCGACGGAGAAAAAAGGGAATCTTGAAGCTTTGAAGGAGTTTGGGCCCACTTGTTTGTCCAAGTGCTATTTGCCATATAAGTTGAATTATCTTTTGACCGCCGTTCGCCGTGACAAGTGCACCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

377

Amino Acids

43.15

Weight (kDa)

5.55

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOP6A-Spo11_Toprim PF21180 196 - 363 8.7e-48 Topoisomerase 6 subunit A/Spo11, Toprim domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014631)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05450
malus_domestica MD02G1062600.v1.1 MD15G1190400.v1.1
prunus_persica Prupe.7G226700_v2.0.a1
pyrus_communis pycom02g04330 pycom15g17060
rosa_chinensis RchiOBHm_Chr2g0091121
rosa_laevigata RLG00000016181
rosa_multiflora Rmu_sc0004851.1_g000008
rosa_roxburghii Rroxscaffold_2G00150530
rosa_rugosa Rorug02G0013100 Rorug02G0013200
rosa_samantha Rh2AG059400 Rh2BG057800 Rh2CG059600 Rh2DG058700
rosa_wichuraiana Rw2G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 746
AciI CCGC 1 cut(s) 1104
AclWI GGATC 2 cut(s) 253, 758
AcoI YGGCCR 2 cut(s) 534, 869
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 4 cut(s) 5, 90, 380, 500
AfiI CCNNNNNNNGG 2 cut(s) 268, 746
AgsI TTSAA 7 cut(s) 104, 426, 568, 949, 1031, 1039, 1090
AluBI AGCT 6 cut(s) 107, 135, 242, 715, 991, 1034
AluI AGCT 6 cut(s) 107, 135, 242, 715, 991, 1034
Alw21I GWGCWC 1 cut(s) 1127
Alw26I GTCTC 2 cut(s) 434, 924
Alw44I GTGCAC 1 cut(s) 1123
AlwI GGATC 2 cut(s) 253, 758
Ama87I CYCGRG 2 cut(s) 284, 686
AoxI GGCC 5 cut(s) 534, 748, 816, 869, 1049
ApaI GGGCCC 1 cut(s) 1053
ApaLI GTGCAC 1 cut(s) 1123
ApeKI GCWGC 2 cut(s) 414, 417
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 2 cut(s) 1049, 1050
AsuC2I CCSGG 3 cut(s) 521, 687, 688
AsuHPI GGTGA 2 cut(s) 188, 661
AvaI CYCGRG 2 cut(s) 284, 686
BaeGI GKGCMC 2 cut(s) 1053, 1127
BalI TGGCCA 1 cut(s) 536
BanII GRGCYC 1 cut(s) 1053
BauI CACGAG 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 1127
BbvI GCAGC 2 cut(s) 401, 429
BccI CCATC 5 cut(s) 122, 182, 262, 521, 545
BceAI ACGGC 2 cut(s) 1091, 1098
BcnI CCSGG 3 cut(s) 521, 687, 688
BcoDI GTCTC 2 cut(s) 434, 924
BfaI CTAG 2 cut(s) 152, 447
BfmI CTRYAG 3 cut(s) 78, 408, 415
BisI GCNGC 2 cut(s) 415, 418
BlsI GCNGC 2 cut(s) 416, 419
Bme1390I CCNGG 3 cut(s) 521, 687, 688
BmeT110I CYCGRG 2 cut(s) 284, 686
BmgT120I GGNCC 2 cut(s) 1049, 1050
BmiI GGNNCC 2 cut(s) 580, 1051
BmrFI CCNGG 3 cut(s) 521, 687, 688
BmsI GCATC 2 cut(s) 433, 783
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 3 cut(s) 521, 687, 688
BsaAI YACGTR 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 3 cut(s) 686, 687, 974
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 746
Bse1I ACTGG 3 cut(s) 351, 612, 740
Bse8I GATNNNNATC 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 686, 687, 974
BseGI GGATG 2 cut(s) 529, 537
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 268, 746
BseNI ACTGG 3 cut(s) 351, 612, 740
BseRI GAGGAG 3 cut(s) 389, 827, 1001
BseSI GKGCMC 2 cut(s) 1053, 1127
BseXI GCAGC 2 cut(s) 401, 429
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 5 cut(s) 536, 750, 818, 871, 1051
BsiHKAI GWGCWC 1 cut(s) 1127
BsiHKCI CYCGRG 2 cut(s) 284, 686
BsiSI CCGG 3 cut(s) 520, 672, 687
BslI CCNNNNNNNGG 2 cut(s) 268, 746
BsmAI GTCTC 2 cut(s) 434, 924
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 5 cut(s) 536, 750, 818, 871, 1051
BsoBI CYCGRG 2 cut(s) 284, 686
Bsp120I GGGCCC 1 cut(s) 1049
Bsp1286I GDGCHC 2 cut(s) 1053, 1127
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 46, 258, 274, 763
BspACI CCGC 1 cut(s) 1104
BspANI GGCC 5 cut(s) 536, 750, 818, 871, 1051
BspLI GGNNCC 2 cut(s) 580, 1051
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 2 cut(s) 253, 758
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 351, 612, 740
BssECI CCNNGG 3 cut(s) 686, 687, 974
BssMI GATC 4 cut(s) 46, 258, 274, 763
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 59
Bst4CI ACNGT 4 cut(s) 208, 647, 761, 799
BstACI GRCGYC 1 cut(s) 192
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 772
BstF5I GGATG 2 cut(s) 529, 537
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 4 cut(s) 49, 261, 277, 766
BstMAI GTCTC 2 cut(s) 434, 924
BstMBI GATC 4 cut(s) 46, 258, 274, 763
BstMWI GCNNNNNNNGC 1 cut(s) 411
BstSCI CCNGG 3 cut(s) 519, 685, 686
BstSFI CTRYAG 3 cut(s) 78, 408, 415
BstSLI GKGCMC 2 cut(s) 1053, 1127
BstSNI TACGTA 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 401, 429
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 5 cut(s) 536, 750, 818, 871, 1051
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 2 cut(s) 529, 537
BtsIMutI CAGTG 2 cut(s) 213, 747
Cac8I GCNNGC 1 cut(s) 772
CfoI GCGC 1 cut(s) 889
Cfr13I GGNCC 2 cut(s) 1049, 1050
Cfr9I CCCGGG 1 cut(s) 686
CseI GACGC 2 cut(s) 41, 181
Csp6I GTAC 4 cut(s) 4, 89, 379, 499
CviAII CATG 5 cut(s) 547, 628, 657, 855, 934
CviQI GTAC 4 cut(s) 4, 89, 379, 499
DpnI GATC 4 cut(s) 48, 260, 276, 765
DpnII GATC 4 cut(s) 46, 258, 274, 763
DraI TTTAAA 1 cut(s) 711
EaeI YGGCCR 2 cut(s) 534, 869
Eco105I TACGTA 1 cut(s) 334
Eco147I AGGCCT 1 cut(s) 818
Eco24I GRGCYC 1 cut(s) 1053
Eco88I CYCGRG 2 cut(s) 284, 686
EcoT22I ATGCAT 2 cut(s) 552, 662
EcoT38I GRGCYC 1 cut(s) 1053
FaeI CATG 5 cut(s) 550, 631, 660, 858, 937
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FatI CATG 5 cut(s) 546, 627, 656, 854, 933
FauNDI CATATG 1 cut(s) 808
Fnu4HI GCNGC 2 cut(s) 415, 418
FokI GGATG 2 cut(s) 524, 536
FriOI GRGCYC 1 cut(s) 1053
Fsp4HI GCNGC 2 cut(s) 415, 418
FspBI CTAG 2 cut(s) 152, 447
GlaI GCGC 1 cut(s) 888
GluI GCNGC 2 cut(s) 415, 418
HaeIII GGCC 5 cut(s) 536, 750, 818, 871, 1051
HapII CCGG 3 cut(s) 520, 672, 687
HgaI GACGC 2 cut(s) 41, 181
HhaI GCGC 1 cut(s) 889
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 5 cut(s) 550, 631, 660, 858, 937
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HindIII AAGCTT 1 cut(s) 1032
HinfI GANTC 2 cut(s) 913, 1024
HpaII CCGG 3 cut(s) 520, 672, 687
HphI GGTGA 2 cut(s) 188, 661
Hpy166II GTNNAC 4 cut(s) 6, 363, 832, 1125
Hpy188I TCNGA 3 cut(s) 556, 724, 975
Hpy188III TCNNGA 4 cut(s) 59, 112, 152, 1028
Hpy8I GTNNAC 4 cut(s) 6, 363, 832, 1125
Hpy99I CGWCG 2 cut(s) 908, 1012
HpyAV CCTTC 3 cut(s) 205, 829, 1033
HpyCH4III ACNGT 4 cut(s) 208, 647, 761, 799
HpyCH4IV ACGT 4 cut(s) 165, 199, 333, 453
HpyCH4V TGCA 7 cut(s) 405, 417, 490, 550, 631, 660, 1125
HpyF10VI GCNNNNNNNGC 1 cut(s) 411
HpySE526I ACGT 4 cut(s) 165, 199, 333, 453
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 5 cut(s) 550, 631, 660, 858, 937
HspAI GCGC 1 cut(s) 887
Kzo9I GATC 4 cut(s) 46, 258, 274, 763
LmnI GCTCC 4 cut(s) 40, 132, 578, 988
Lsp1109I GCAGC 2 cut(s) 401, 429
LweI GCATC 2 cut(s) 433, 783
MaeI CTAG 2 cut(s) 152, 447
MaeII ACGT 4 cut(s) 165, 199, 333, 453
MaeIII GTNAC 5 cut(s) 56, 166, 449, 875, 1115
MalI GATC 4 cut(s) 48, 260, 276, 765
MboI GATC 4 cut(s) 46, 258, 274, 763
MboII GAAGA 4 cut(s) 56, 283, 714, 965
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 2 cut(s) 1053, 1127
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 7 cut(s) 22, 119, 384, 571, 727, 1090, 1129
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 922
MmeI TCCRAC 4 cut(s) 122, 186, 448, 702
Mox20I TGGCCA 1 cut(s) 536
Mph1103I ATGCAT 2 cut(s) 552, 662
MscI TGGCCA 1 cut(s) 536
MseI TTAA 4 cut(s) 390, 710, 836, 1132
MslI CAYNNNNRTG 2 cut(s) 674, 779
Msp20I TGGCCA 1 cut(s) 536
MspI CCGG 3 cut(s) 520, 672, 687
MspR9I CCNGG 3 cut(s) 521, 687, 688
Mva1269I GAATGC 1 cut(s) 550
MwoI GCNNNNNNNGC 1 cut(s) 411
NciI CCSGG 3 cut(s) 521, 687, 688
NdeI CATATG 1 cut(s) 808
NdeII GATC 4 cut(s) 46, 258, 274, 763
NlaIII CATG 5 cut(s) 550, 631, 660, 858, 937
NlaIV GGNNCC 2 cut(s) 580, 1051
NmeAIII GCCGAG 1 cut(s) 897
NmuCI GTSAC 5 cut(s) 56, 166, 449, 875, 1115
NsiI ATGCAT 2 cut(s) 552, 662
PceI AGGCCT 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 1 cut(s) 550
PfeI GAWTC 1 cut(s) 1024
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 2 cut(s) 416, 419
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
Ppu21I YACGTR 1 cut(s) 334
PsiI TTATAA 1 cut(s) 621
PspN4I GGNNCC 2 cut(s) 580, 1051
PspOMI GGGCCC 1 cut(s) 1049
PspPI GGNCC 2 cut(s) 1049, 1050
PstI CTGCAG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 4 cut(s) 5, 90, 380, 500
RsaNI GTAC 4 cut(s) 4, 89, 379, 499
RseI CAYNNNNRTG 2 cut(s) 674, 779
SaqAI TTAA 4 cut(s) 390, 710, 836, 1132
SatI GCNGC 2 cut(s) 415, 418
Sau3AI GATC 4 cut(s) 46, 258, 274, 763
Sau96I GGNCC 2 cut(s) 1049, 1050
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 3 cut(s) 521, 687, 688
SduI GDGCHC 2 cut(s) 1053, 1127
SfaNI GCATC 2 cut(s) 433, 783
SfcI CTRYAG 3 cut(s) 78, 408, 415
SmaI CCCGGG 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 674, 779
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SnaBI TACGTA 1 cut(s) 334
Sse9I AATT 7 cut(s) 22, 119, 384, 571, 727, 1090, 1129
SseBI AGGCCT 1 cut(s) 818
SsiI CCGC 1 cut(s) 1104
SspMI CTAG 2 cut(s) 152, 447
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 3 cut(s) 519, 685, 686
TaaI ACNGT 4 cut(s) 208, 647, 761, 799
TaiI ACGT 4 cut(s) 168, 202, 336, 456
TaqI TCGA 5 cut(s) 266, 514, 756, 788, 822
TasI AATT 7 cut(s) 22, 119, 384, 571, 727, 1090, 1129
TatI WGTACW 2 cut(s) 3, 88
TfiI GAWTC 1 cut(s) 1024
Tru1I TTAA 4 cut(s) 390, 710, 836, 1132
Tru9I TTAA 4 cut(s) 390, 710, 836, 1132
TscAI CASTG 2 cut(s) 213, 747
TseFI GTSAC 5 cut(s) 56, 166, 449, 875, 1115
TseI GCWGC 2 cut(s) 414, 417
Tsp45I GTSAC 5 cut(s) 56, 166, 449, 875, 1115
TspDTI ATGAA 3 cut(s) 35, 345, 1016
TspGWI ACGGA 2 cut(s) 251, 1025
TspMI CCCGGG 1 cut(s) 686
TspRI CASTG 2 cut(s) 213, 747
Van91I CCANNNNNTGG 1 cut(s) 746
VneI GTGCAC 1 cut(s) 1123
XbaI TCTAGA 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmaI CCCGGG 1 cut(s) 686
XspI CTAG 2 cut(s) 152, 447
Zsp2I ATGCAT 2 cut(s) 552, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.