MD02G1079700.v1.1

Transmembrane protein 258-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015445
Physical Location & Seq
Reverse (-)
6278721 .. 6280627
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1079700.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGAGCCAGACCCGCCAAATCACCTACATTTGCTACCGAACACCCACACGATTGTAGCCTCCTCTGTCAAATTTGACATAGAGCCTTCCCTTCCTGTACTCTCACTCGCCAAAGTCGCTGCTTCGATCTGTAGAGATCAAGGACCGAAGCTTTCAGCAACTTCCAACTCGCCGGCTACGATGGCTTCGAAGGCAATTTCCAGTCCCGTGCCGGTCACGTGGTACCCGACTCTCGCCGTCGTGATGGTCTCCCTCGGCCTCCTCTTCACCGCCTCCTTCTTCATCTATGAGGCAACCATTTCCAGGAAAAACCGCAGTCTTGCGAAGGAAGTCATAACAGCAACATTCGCTTCCTTCTTTTTGGGTTTTGGATCCTTGTTCTTGCTGCTTGCATCGGGCGTTTATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ost5

Protein Analysis

137

Amino Acids

14.51

Weight (kDa)

8.85

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ost5 PF05251 65 - 136 8.2e-24 Oligosaccharyltransferase subunit 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29735 AT4G29735
fragaria_vesca FvH4_1g07510
malus_domestica MD02G1079700.v1.1 MD15G1207400.v1.1
prunus_persica Prupe.7G209400_v2.0.a1
pyrus_communis pycom02g06350
rosa_chinensis RchiOBHm_Chr2g0093401
rosa_multiflora Rmu_sc0005227.1_g000016 Rmu_sc0032382.1_g000002
rosa_rugosa Rorug02G0035100
rosa_samantha Rh2AG081500 Rh2BG082800 Rh2CG084800 Rh2DG080500
rosa_wichuraiana Rw2G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AciI CCGC 3 cut(s) 14, 270, 313
AclWI GGATC 2 cut(s) 366, 379
AcsI RAATTY 1 cut(s) 71
AcvI CACGTG 1 cut(s) 219
AfaI GTAC 2 cut(s) 99, 224
AfiI CCNNNNNNNGG 1 cut(s) 303
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 1 cut(s) 151
AluI AGCT 1 cut(s) 151
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 2 cut(s) 366, 379
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 2 cut(s) 119, 385
ApoI RAATTY 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 14, 259
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 371
BanI GGYRCC 1 cut(s) 222
BbrPI CACGTG 1 cut(s) 219
BbvI GCAGC 2 cut(s) 106, 372
BccI CCATC 2 cut(s) 175, 238
BceAI ACGGC 1 cut(s) 221
BciT130I CCWGG 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 253
BfmI CTRYAG 1 cut(s) 130
BisI GCNGC 2 cut(s) 120, 386
BlsI GCNGC 2 cut(s) 121, 387
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 3 cut(s) 6, 224, 373
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 401
Bpu14I TTCGAA 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 253
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse118I RCCGGY 2 cut(s) 172, 211
Bse1I ACTGG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 52, 251
BseXI GCAGC 2 cut(s) 106, 372
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 173, 212
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 1 cut(s) 258
Bso31I GGTCTC 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 188
Bsp143I GATC 3 cut(s) 126, 136, 371
BspACI CCGC 3 cut(s) 14, 270, 313
BspANI GGCC 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 6, 224, 373
BspPI GGATC 2 cut(s) 366, 379
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 253
BsrFI RCCGGY 2 cut(s) 172, 211
BsrI ACTGG 1 cut(s) 201
BssAI RCCGGY 2 cut(s) 172, 211
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 126, 136, 371
Bst2UI CCWGG 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 174, 390
BstKTI GATC 3 cut(s) 129, 139, 374
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 3 cut(s) 126, 136, 371
BstMWI GCNNNNNNNGC 5 cut(s) 13, 116, 182, 191, 347
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 106, 372
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 174, 390
Cfr10I RCCGGY 2 cut(s) 172, 211
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 98, 223
CviJI RGCY 7 cut(s) 7, 59, 85, 151, 176, 185, 258
CviKI_1 RGCY 7 cut(s) 7, 59, 85, 151, 176, 185, 258
CviQI GTAC 2 cut(s) 98, 223
DpnI GATC 3 cut(s) 128, 138, 373
DpnII GATC 3 cut(s) 126, 136, 371
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 302
FaiI YATR 4 cut(s) 80, 288, 335, 405
FaqI GGGAC 1 cut(s) 189
FauI CCCGC 1 cut(s) 21
Fnu4HI GCNGC 2 cut(s) 120, 386
Fsp4HI GCNGC 2 cut(s) 120, 386
GluI GCNGC 2 cut(s) 120, 386
HaeIII GGCC 1 cut(s) 258
HapII CCGG 2 cut(s) 173, 212
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 1 cut(s) 229
HpaII CCGG 2 cut(s) 173, 212
HphI GGTGA 2 cut(s) 14, 259
Hpy188I TCNGA 1 cut(s) 410
Hpy188III TCNNGA 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 6 cut(s) 96, 101, 184, 286, 319, 364
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 1 cut(s) 392
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 116, 182, 191, 347
HpySE526I ACGT 1 cut(s) 218
KpnI GGTACC 1 cut(s) 226
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
Kzo9I GATC 3 cut(s) 126, 136, 371
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 21, 108, 186, 214, 225, 289, 316
Lsp1109I GCAGC 2 cut(s) 106, 372
LweI GCATC 1 cut(s) 401
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 128, 138, 373
MboI GATC 3 cut(s) 126, 136, 371
MboII GAAGA 2 cut(s) 256, 271
MflI RGATCY 1 cut(s) 371
MluCI AATT 2 cut(s) 71, 195
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 7 cut(s) 70, 73, 263, 269, 272, 283, 283
MroNI GCCGGC 1 cut(s) 172
MspI CCGG 2 cut(s) 173, 212
MspR9I CCNGG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 5 cut(s) 13, 116, 182, 191, 347
NaeI GCCGGC 1 cut(s) 174
NdeII GATC 3 cut(s) 126, 136, 371
NgoMIV GCCGGC 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 6, 224, 373
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 214
NspV TTCGAA 1 cut(s) 188
PcsI WCGNNNNNNNCGW 3 cut(s) 176, 185, 224
PdiI GCCGGC 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 2 cut(s) 121, 387
PleI GAGTC 1 cut(s) 223
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 302
PspCI CACGTG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 3 cut(s) 6, 224, 373
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 99, 224
RsaNI GTAC 2 cut(s) 98, 223
SatI GCNGC 2 cut(s) 120, 386
Sau3AI GATC 3 cut(s) 126, 136, 371
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 304
SetI ASST 3 cut(s) 27, 153, 221
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 143
Sse9I AATT 2 cut(s) 71, 195
SsiI CCGC 3 cut(s) 14, 270, 313
StyD4I CCNGG 1 cut(s) 302
TaiI ACGT 1 cut(s) 221
TaqI TCGA 2 cut(s) 125, 188
TaqII GACCGA 1 cut(s) 160
TasI AATT 2 cut(s) 71, 195
TatI WGTACW 1 cut(s) 97
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 119, 385
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.