pycom02g06350

Transmembrane protein 258-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015445
Physical Location & Seq
Reverse (-)
4409209 .. 4410777
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06350.2

Sequence Viewer

Length: 462 bp
ATGAAAATAATACAGACTCCAAACGCTGCGTTTAGGAGACCAACCGTAGTGATGGAGCCAGACCCGCCAAATCACCTACGTTTGGTACCGAACACCCACAGGATTCTAGCCTCCTCCATCAAATTTGACATAGAGCCTTCCCTTCCTGTACTCTCAGTCGCCAAAGTCTCTGCTTCGATCTGTAGAGATCAAAGACCGAAGCTTTCAGCAACTTCCAACTCGCCGGCTACGATGGCTTCGAAGGCAATTTCCAGTCCCGTGCCCGTCACGTGGTACCCGACTCTCGCCGTCGTGATGGTCTCCCTCGGTCTCCTCTTCACCGCCTCCTTCTTCATCTATGAGGCTACCATTTCTCGGAAAAACCGCAGTCTTGCAAAGGAAGTCATAACAGCAACATTCGCTTCCTTCTTTTTGGGTTTCGGATCCTTGTTCTTGCTGCTTGCATCGGGCGTTTATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ost5

Protein Analysis

154

Amino Acids

16.63

Weight (kDa)

10.17

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29735 AT4G29735
fragaria_vesca FvH4_1g07510
malus_domestica MD02G1079700.v1.1 MD15G1207400.v1.1
prunus_persica Prupe.7G209400_v2.0.a1
pyrus_communis pycom02g06350
rosa_chinensis RchiOBHm_Chr2g0093401
rosa_multiflora Rmu_sc0005227.1_g000016 Rmu_sc0032382.1_g000002
rosa_rugosa Rorug02G0035100
rosa_samantha Rh2AG081500 Rh2BG082800 Rh2CG084800 Rh2DG080500
rosa_wichuraiana Rw2G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 85, 273
AccB1I GGYRCC 2 cut(s) 85, 273
AciI CCGC 3 cut(s) 65, 321, 364
AclWI GGATC 2 cut(s) 417, 430
AcsI RAATTY 1 cut(s) 122
AcvI CACGTG 1 cut(s) 270
AfaI GTAC 3 cut(s) 87, 150, 275
AfiI CCNNNNNNNGG 3 cut(s) 82, 270, 354
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 4 cut(s) 31, 172, 304, 314
AlwI GGATC 2 cut(s) 417, 430
ApeKI GCWGC 2 cut(s) 26, 436
ApoI RAATTY 1 cut(s) 122
Asp718I GGTACC 2 cut(s) 85, 273
AsuHPI GGTGA 2 cut(s) 65, 310
AsuII TTCGAA 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 264
BamHI GGATCC 1 cut(s) 422
BanI GGYRCC 2 cut(s) 85, 273
BbrPI CACGTG 1 cut(s) 270
BbvI GCAGC 2 cut(s) 13, 423
BccI CCATC 4 cut(s) 46, 125, 226, 289
BceAI ACGGC 1 cut(s) 272
BcoDI GTCTC 4 cut(s) 31, 172, 304, 314
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 181
BisI GCNGC 2 cut(s) 27, 437
BlsI GCNGC 2 cut(s) 28, 438
BmiI GGNNCC 4 cut(s) 57, 87, 275, 424
BmsI GCATC 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 270
BsaI GGTCTC 3 cut(s) 31, 304, 314
BsaJI CCNNGG 1 cut(s) 304
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 270, 354
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 304
BseLI CCNNNNNNNGG 3 cut(s) 82, 270, 354
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 2 cut(s) 103, 302
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 2 cut(s) 13, 423
BshNI GGYRCC 2 cut(s) 85, 273
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 240
BslI CCNNNNNNNGG 3 cut(s) 82, 270, 354
BsmAI GTCTC 4 cut(s) 31, 172, 304, 314
BsmFI GGGAC 1 cut(s) 240
Bso31I GGTCTC 3 cut(s) 31, 304, 314
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 177, 187, 422
BspACI CCGC 3 cut(s) 65, 321, 364
BspCNI CTCAG 1 cut(s) 167
BspLI GGNNCC 4 cut(s) 57, 87, 275, 424
BspPI GGATC 2 cut(s) 417, 430
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 85, 273
BspTNI GGTCTC 3 cut(s) 31, 304, 314
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 3 cut(s) 177, 187, 422
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 270
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 225, 441
BstDEI CTNAG 1 cut(s) 154
BstKTI GATC 3 cut(s) 180, 190, 425
BstMAI GTCTC 4 cut(s) 31, 172, 304, 314
BstMBI GATC 3 cut(s) 177, 187, 422
BstMWI GCNNNNNNNGC 4 cut(s) 64, 233, 242, 398
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 13, 423
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 225, 441
Cfr10I RCCGGY 1 cut(s) 223
Csp6I GTAC 3 cut(s) 86, 149, 274
CviJI RGCY 7 cut(s) 58, 110, 136, 202, 227, 236, 344
CviKI_1 RGCY 7 cut(s) 58, 110, 136, 202, 227, 236, 344
CviQI GTAC 3 cut(s) 86, 149, 274
DdeI CTNAG 1 cut(s) 154
DpnI GATC 3 cut(s) 179, 189, 424
DpnII GATC 3 cut(s) 177, 187, 422
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Eco31I GGTCTC 3 cut(s) 31, 304, 314
Eco72I CACGTG 1 cut(s) 270
FaiI YATR 4 cut(s) 131, 339, 386, 456
FaqI GGGAC 1 cut(s) 240
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 27, 437
Fsp4HI GCNGC 2 cut(s) 27, 437
FspBI CTAG 1 cut(s) 107
GluI GCNGC 2 cut(s) 27, 437
HapII CCGG 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 3 cut(s) 16, 103, 280
HpaII CCGG 1 cut(s) 224
HphI GGTGA 2 cut(s) 65, 310
Hpy188I TCNGA 3 cut(s) 357, 422, 461
Hpy188III TCNNGA 1 cut(s) 292
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 5 cut(s) 147, 152, 235, 337, 415
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 79, 269
HpyCH4V TGCA 2 cut(s) 374, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 233, 242, 398
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 2 cut(s) 79, 269
KpnI GGTACC 2 cut(s) 89, 277
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Kzo9I GATC 3 cut(s) 177, 187, 422
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 5 cut(s) 72, 85, 159, 237, 265
Lsp1109I GCAGC 2 cut(s) 13, 423
LweI GCATC 1 cut(s) 452
MaeI CTAG 1 cut(s) 107
MaeII ACGT 2 cut(s) 79, 269
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 179, 189, 424
MboI GATC 3 cut(s) 177, 187, 422
MboII GAAGA 2 cut(s) 307, 322
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 2 cut(s) 122, 246
MlyI GAGTC 2 cut(s) 10, 274
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 6 cut(s) 121, 124, 314, 323, 334, 334
MroNI GCCGGC 1 cut(s) 223
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 4 cut(s) 64, 233, 242, 398
NaeI GCCGGC 1 cut(s) 225
NdeII GATC 3 cut(s) 177, 187, 422
NgoMIV GCCGGC 1 cut(s) 223
NlaIV GGNNCC 4 cut(s) 57, 87, 275, 424
NmuCI GTSAC 1 cut(s) 265
NspV TTCGAA 1 cut(s) 239
PcsI WCGNNNNNNNCGW 3 cut(s) 227, 236, 275
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 28, 438
PleI GAGTC 2 cut(s) 10, 274
PmaCI CACGTG 1 cut(s) 270
PmlI CACGTG 1 cut(s) 270
PpsI GAGTC 2 cut(s) 10, 274
Ppu21I YACGTR 1 cut(s) 270
PspCI CACGTG 1 cut(s) 270
PspN4I GGNNCC 4 cut(s) 57, 87, 275, 424
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 3 cut(s) 87, 150, 275
RsaNI GTAC 3 cut(s) 86, 149, 274
SatI GCNGC 2 cut(s) 27, 437
Sau3AI GATC 3 cut(s) 177, 187, 422
SchI GAGTC 2 cut(s) 10, 274
SduI GDGCHC 1 cut(s) 264
SetI ASST 4 cut(s) 78, 82, 204, 272
SfaNI GCATC 1 cut(s) 452
SfcI CTRYAG 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 239
Sse9I AATT 2 cut(s) 122, 246
SsiI CCGC 3 cut(s) 65, 321, 364
SspMI CTAG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 2 cut(s) 82, 272
TaqI TCGA 2 cut(s) 176, 239
TaqII GACCGA 2 cut(s) 211, 296
TasI AATT 2 cut(s) 122, 246
TatI WGTACW 1 cut(s) 148
TfiI GAWTC 1 cut(s) 103
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 26, 436
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 17, 322
XapI RAATTY 1 cut(s) 122
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.