MD02G1087700.v1.1
MYB Family

transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017380
Physical Location & Seq
Forward (+)
6903721 .. 6905572
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1087700.v1.1.491

Sequence Viewer

Length: 915 bp
ATGGGAAGAAAACCATGCTCTGATAAAGAAGGGCTAAACAGGGGAGCATGGTTAGCTTGGGAAGATAAAATCCTTACCAACTACATTGAAACAAATGGAGAAGGAAAATGGCGAGACCTTCCTCGAAGAGCTGGGTTAAACCGGTGTGGAAAGAGCTGCAGACTGCGATGGTTGAACTATCTGAGGCCTGACATTAAGAGAGGTAACATTTCCAGTGAAGAAGAAGAGCTCATCATTAGACTACATAAGCTTCTTGGAAACAGGTGGTCATTGATCGCTGGAAGACTGCCGGGACGAACCGACAATGAGATCAAGAACTACTGGAACACCACTCTGAGCAAGAAAACGGAGCAAGGAAGCGACAAGGCTCCTCATCATGAACTTACTAGTACTACTAACTCAAAAGAGTACACACCAAAACCGAAACCAGCATCAAAGTCTCATCAGACGGTCCAGGTGATTCGAACGAAAGCGTTTAAGTGCAGGAAGGTTGTCATCCCATCACATTTGGACGGTCACGATCAAATGATTGATAGGTTAAATGTAAACCCTGGTTTGTTCCCGTGGGAAAGCCCGAGTTCATCAGCGTCTCAGGATCCGCAGGAGGACTCACCGCCTTGTGGTTTTCTCATAGATTTCGATATCGATGACCTTTTCATATCTGATTTATTAAATACAGATAATTTTCTCCAAGATCAAATCAATAAGTGTGATCAAGAAATGGAAAATGGAATCACCAAGCTTGAAGGTTTGATGGGTGAAGCAATGTTGGAAAATTGGACCGCTAGTGTTCATGATCATCACGATGACCAGATATTTCAGCCAAATGATGATCATGATCGTGTCCATGGCCTCAATGCAACTTCATCTGTTCTGAATTTAGAAGACGATCATGAAAAGTGGAGCAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

305

Amino Acids

34.78

Weight (kDa)

5.75

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.6e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 76 3.8e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 120 5.5e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017380)

Species Orthologous Gene IDs
malus_domestica MD02G1087700.v1.1 MD15G1215400.v1.1
prunus_persica Prupe.7G201700_v2.0.a1 Prupe.7G201800_v2.0.a1
pyrus_communis pycom02g07050
rosa_chinensis RchiOBHm_Chr2g0094481
rosa_laevigata RLG00000016494
rosa_multiflora Rmu_sc0004359.1_g000049
rosa_roxburghii Rroxscaffold_2G00147140
rosa_samantha Rh2AG092400 Rh2BG092600 Rh2CG095100 Rh2DG091400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 599, 614, 783
AclWI GGATC 2 cut(s) 590, 603
AcsI RAATTY 1 cut(s) 877
AfaI GTAC 2 cut(s) 391, 410
AfiI CCNNNNNNNGG 1 cut(s) 620
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 3 cut(s) 89, 175, 746
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 2 cut(s) 453, 550
AluBI AGCT 6 cut(s) 56, 131, 156, 229, 250, 742
AluI AGCT 6 cut(s) 56, 131, 156, 229, 250, 742
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 3 cut(s) 108, 444, 594
AlwI GGATC 2 cut(s) 590, 603
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 2 cut(s) 185, 850
ApeKI GCWGC 1 cut(s) 156
ApoI RAATTY 1 cut(s) 877
AsiGI ACCGGT 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 451, 780
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 469, 603, 727, 770
AsuII TTCGAA 1 cut(s) 463
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 2 cut(s) 451, 780
BamHI GGATCC 1 cut(s) 595
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 2 cut(s) 289, 891
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 143
BccI CCATC 3 cut(s) 162, 508, 748
BciT130I CCWGG 2 cut(s) 455, 552
BclI TGATCA 3 cut(s) 712, 796, 832
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 3 cut(s) 108, 444, 594
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 2 cut(s) 387, 786
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 3 cut(s) 291, 455, 552
Bme18I GGWCC 2 cut(s) 451, 780
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 451, 780
BmiI GGNNCC 2 cut(s) 369, 597
BmrFI CCNGG 3 cut(s) 291, 455, 552
BmsI GCATC 1 cut(s) 440
BpiI GAAGAC 2 cut(s) 289, 891
Bpu14I TTCGAA 1 cut(s) 463
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 645
BsaBI GATNNNNATC 1 cut(s) 837
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 550, 563, 847
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 213, 326
Bse3DI GCAATG 1 cut(s) 771
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 2 cut(s) 455, 552
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 3 cut(s) 550, 563, 847
BseGI GGATG 1 cut(s) 495
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 3 cut(s) 173, 326, 605
BseNI ACTGG 2 cut(s) 213, 326
BseRI GAGGAG 1 cut(s) 360
BseXI GCAGC 1 cut(s) 143
BseYI CCCAGC 1 cut(s) 131
BsgI GTGCAG 1 cut(s) 502
BshFI GGCC 2 cut(s) 187, 852
BshTI ACCGGT 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 2 cut(s) 142, 290
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 620
BsmAI GTCTC 3 cut(s) 108, 444, 594
BsmBI CGTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 2 cut(s) 187, 852
Bso31I GGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 574
Bsp119I TTCGAA 1 cut(s) 463
Bsp1286I GDGCHC 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 847
BspACI CCGC 3 cut(s) 599, 614, 783
BspANI GGCC 2 cut(s) 187, 852
BspCNI CTCAG 3 cut(s) 174, 327, 604
BspDI ATCGAT 1 cut(s) 645
BspHI TCATGA 4 cut(s) 376, 793, 835, 892
BspLI GGNNCC 2 cut(s) 369, 597
BspMAI CTGCAG 1 cut(s) 161
BspPI GGATC 2 cut(s) 590, 603
BspQI GCTCTTC 2 cut(s) 121, 219
BspT104I TTCGAA 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 2 cut(s) 213, 326
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 3 cut(s) 550, 563, 847
BssT1I CCWWGG 1 cut(s) 847
Bst2UI CCWGG 2 cut(s) 455, 552
Bst4CI ACNGT 2 cut(s) 451, 515
Bst6I CTCTTC 2 cut(s) 121, 219
BstBI TTCGAA 1 cut(s) 463
BstDEI CTNAG 3 cut(s) 182, 335, 591
BstDSI CCRYGG 2 cut(s) 563, 847
BstF5I GGATG 1 cut(s) 495
BstMAI GTCTC 3 cut(s) 108, 444, 594
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 2 cut(s) 455, 552
BstSCI CCNGG 3 cut(s) 289, 453, 550
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 143
BstV2I GAAGAC 2 cut(s) 289, 891
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 2 cut(s) 187, 852
BsuTUI ATCGAT 1 cut(s) 645
BtgI CCRYGG 2 cut(s) 563, 847
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 220
CciI TCATGA 4 cut(s) 376, 793, 835, 892
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 451, 780
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 1 cut(s) 576
Csp6I GTAC 2 cut(s) 390, 409
CspAI ACCGGT 1 cut(s) 141
CviAII CATG 7 cut(s) 15, 48, 377, 794, 836, 848, 893
CviQI GTAC 2 cut(s) 390, 409
DdeI CTNAG 3 cut(s) 182, 335, 591
Eam1104I CTCTTC 2 cut(s) 121, 219
EarI CTCTTC 2 cut(s) 121, 219
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 847
Eco147I AGGCCT 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 108
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 451, 780
Eco53kI GAGCTC 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 229
EcoRII CCWGG 2 cut(s) 453, 550
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 847
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 847
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 7 cut(s) 18, 51, 380, 797, 839, 851, 896
FaqI GGGAC 1 cut(s) 306
FatI CATG 7 cut(s) 14, 47, 376, 793, 835, 847, 892
FbaI TGATCA 3 cut(s) 712, 796, 832
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 157
FspBI CTAG 2 cut(s) 387, 786
GluI GCNGC 1 cut(s) 157
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 2 cut(s) 187, 852
HapII CCGG 2 cut(s) 142, 290
HgaI GACGC 1 cut(s) 576
Hin1II CATG 7 cut(s) 18, 51, 380, 797, 839, 851, 896
HindIII AAGCTT 2 cut(s) 248, 740
HinfI GANTC 3 cut(s) 460, 608, 732
HpaII CCGG 2 cut(s) 142, 290
HphI GGTGA 4 cut(s) 469, 603, 727, 770
Hpy166II GTNNAC 2 cut(s) 411, 547
Hpy188I TCNGA 6 cut(s) 22, 183, 336, 447, 664, 876
Hpy188III TCNNGA 9 cut(s) 313, 377, 518, 593, 716, 794, 803, 836, 893
Hpy8I GTNNAC 2 cut(s) 411, 547
HpyAV CCTTC 5 cut(s) 23, 95, 128, 481, 740
HpyCH4III ACNGT 2 cut(s) 451, 515
HpyCH4V TGCA 3 cut(s) 159, 483, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 3 cut(s) 182, 335, 591
Hsp92II CATG 7 cut(s) 18, 51, 380, 797, 839, 851, 896
Ksp22I TGATCA 3 cut(s) 712, 796, 832
LguI GCTCTTC 2 cut(s) 121, 219
LmnI GCTCC 4 cut(s) 44, 349, 373, 903
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 1 cut(s) 440
MaeI CTAG 2 cut(s) 387, 786
MaeIII GTNAC 2 cut(s) 203, 515
MboII GAAGA 8 cut(s) 18, 74, 138, 230, 233, 236, 294, 896
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 4 cut(s) 682, 775, 877, 910
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 6 cut(s) 132, 177, 194, 381, 598, 863
MseI TTAA 5 cut(s) 137, 195, 477, 539, 671
MslI CAYNNNNRTG 2 cut(s) 804, 840
MspI CCGG 2 cut(s) 142, 290
MspR9I CCNGG 3 cut(s) 291, 455, 552
MvaI CCWGG 2 cut(s) 455, 552
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 1 cut(s) 847
NlaIII CATG 7 cut(s) 18, 51, 380, 797, 839, 851, 896
NlaIV GGNNCC 2 cut(s) 369, 597
NmuCI GTSAC 1 cut(s) 515
NspV TTCGAA 1 cut(s) 463
PagI TCATGA 4 cut(s) 376, 793, 835, 892
PceI AGGCCT 1 cut(s) 187
PciSI GCTCTTC 2 cut(s) 121, 219
PfeI GAWTC 2 cut(s) 460, 732
PinAI ACCGGT 1 cut(s) 141
PkrI GCNGC 1 cut(s) 158
PleI GAGTC 1 cut(s) 602
PpsI GAGTC 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 453, 550
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 2 cut(s) 453, 550
PspN4I GGNNCC 2 cut(s) 369, 597
PspPI GGNCC 2 cut(s) 451, 780
PstI CTGCAG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 2 cut(s) 391, 410
RsaNI GTAC 2 cut(s) 390, 409
RseI CAYNNNNRTG 2 cut(s) 804, 840
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 2 cut(s) 121, 219
SaqAI TTAA 5 cut(s) 137, 195, 477, 539, 671
SatI GCNGC 1 cut(s) 157
Sau96I GGNCC 2 cut(s) 451, 780
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 3 cut(s) 291, 455, 552
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 1 cut(s) 440
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 463
SinI GGWCC 2 cut(s) 451, 780
SmiMI CAYNNNNRTG 2 cut(s) 804, 840
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 4 cut(s) 682, 775, 877, 910
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 3 cut(s) 599, 614, 783
SspMI CTAG 2 cut(s) 387, 786
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 289, 453, 550
StyI CCWWGG 1 cut(s) 847
TaaI ACNGT 2 cut(s) 451, 515
TaqI TCGA 4 cut(s) 124, 463, 639, 645
TasI AATT 4 cut(s) 682, 775, 877, 910
TatI WGTACW 2 cut(s) 389, 408
TfiI GAWTC 2 cut(s) 460, 732
Tru1I TTAA 5 cut(s) 137, 195, 477, 539, 671
Tru9I TTAA 5 cut(s) 137, 195, 477, 539, 671
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 6 cut(s) 393, 570, 646, 782, 855, 909
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 220
VpaK11BI GGWCC 2 cut(s) 451, 780
XapI RAATTY 1 cut(s) 877
XspI CTAG 2 cut(s) 387, 786
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.