MD15G1215400.v1.1
MYB Family

transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017380
Physical Location & Seq
Forward (+)
17357464 .. 17358916
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1215400.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGGGAGAAAACCTTGCTGTGATAAAGAAGGGCTAAACAGGGGAGCATGGTCAGCTTGGGAAGATAAGGCCCTTACCAACTACATTGAAACAAATGGAGAAGGAAAATGGCGAGACCTTCCTCAAAGAGCTGGACTAAACCGGTGTGGAAAGAGCTGTAGACTGCGATGGCTGAATTATCTTAGGCCTGGCATTAAGAGAGGTAACATTTCTAGTGAAGAAGAGGAGCTCATCATTAGACTGCACAAGCTTCTTGGAAACAGGTGGTCATTGATCGCTGGAAGACTGCCGGGGCGAACAGACAATGAGATCAAGAACTACTGGAACACCAGTCTGAGTAAGAAAACGGAACAAGGAAGTGACAAGGCTCCTCATGAGCGTAGTAGTACTACTAACTCAAACGAGTACACACCAAAACCGAAATCAGAATCAAAGTCACATCGGACGGTCCAGGTGATTCGAACAAAAGCATTCAAGTGCAGGAAGATTGTCATCCCATCACATTTGGACGGTCACGATCAAATGGTTGATAGGTCAAATGTAAACCCTGGTTTGGTCCCGTGTGAAAGCCCTAGTCCATCAGGGGCTCAGGATCATCAGGAGGACTCACTGCTGTGTGGTATGCTAATCGATTTCAATATCGATGACCTCATATCTGATTTGCTAAACACAGACAAATTTCTTCAAGATCAAATCAATAAATGTGATCAAGAAATGGAAGATGGAATCACCAAGCTTGAAGATTTGACGGGTGAACCAATGCTGGAAAATTGGACGGCTACTGATCATGATCATCACGATGACCCGATATTTCAGCCAAATGATGATCAAGATCGTGTCGACCGCCTCAATGCAATTTCATATTTTCTGAATTCTGAAGACGGTGATGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

299

Amino Acids

34.0

Weight (kDa)

5.45

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.1e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 2.4e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 110 1.6e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 120 5.4e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017380)

Species Orthologous Gene IDs
malus_domestica MD02G1087700.v1.1 MD15G1215400.v1.1
prunus_persica Prupe.7G201700_v2.0.a1 Prupe.7G201800_v2.0.a1
pyrus_communis pycom02g07050
rosa_chinensis RchiOBHm_Chr2g0094481
rosa_laevigata RLG00000016494
rosa_multiflora Rmu_sc0004359.1_g000049
rosa_roxburghii Rroxscaffold_2G00147140
rosa_samantha Rh2AG092400 Rh2BG092600 Rh2CG095100 Rh2DG091400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 160, 840
AciI CCGC 1 cut(s) 844
AclWI GGATC 1 cut(s) 600
AcsI RAATTY 2 cut(s) 677, 871
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 2 cut(s) 388, 407
AfiI CCNNNNNNNGG 1 cut(s) 553
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 5 cut(s) 89, 475, 637, 686, 740
AjnI CCWGG 3 cut(s) 187, 450, 547
AleI CACNNNNGTG 1 cut(s) 613
AluBI AGCT 6 cut(s) 56, 131, 156, 229, 250, 736
AluI AGCT 6 cut(s) 56, 131, 156, 229, 250, 736
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 1 cut(s) 600
AoxI GGCC 2 cut(s) 69, 185
ApoI RAATTY 2 cut(s) 677, 871
AsiGI ACCGGT 1 cut(s) 141
AspS9I GGNCC 3 cut(s) 70, 448, 556
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 466, 721, 764, 896
AsuII TTCGAA 1 cut(s) 460
AvaII GGWCC 2 cut(s) 448, 556
BanII GRGCYC 2 cut(s) 231, 589
BbsI GAAGAC 2 cut(s) 289, 885
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 4 cut(s) 162, 505, 586, 716
BceAI ACGGC 1 cut(s) 792
BciT130I CCWGG 3 cut(s) 189, 452, 549
BclI TGATCA 4 cut(s) 706, 784, 790, 826
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 2 cut(s) 213, 573
BfmI CTRYAG 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 4 cut(s) 189, 291, 452, 549
Bme18I GGWCC 2 cut(s) 448, 556
BmgT120I GGNCC 3 cut(s) 70, 448, 556
BmiI GGNNCC 2 cut(s) 369, 558
BmrFI CCNGG 4 cut(s) 189, 291, 452, 549
BpiI GAAGAC 2 cut(s) 289, 885
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 2 cut(s) 630, 642
BsaBI GATNNNNATC 3 cut(s) 491, 789, 831
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 290, 547
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 326, 330
Bse8I GATNNNNATC 3 cut(s) 491, 789, 831
BseBI CCWGG 3 cut(s) 189, 452, 549
BseCI ATCGAT 2 cut(s) 630, 642
BseDI CCNNGG 2 cut(s) 290, 547
BseGI GGATG 1 cut(s) 492
BseJI GATNNNNATC 3 cut(s) 491, 789, 831
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMII CTCAG 2 cut(s) 326, 602
BseNI ACTGG 2 cut(s) 326, 330
BseRI GAGGAG 2 cut(s) 239, 360
BsgI GTGCAG 2 cut(s) 227, 499
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 2 cut(s) 71, 187
BshTI ACCGGT 1 cut(s) 141
BshVI ATCGAT 2 cut(s) 630, 642
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 142, 290
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 2 cut(s) 71, 187
Bso31I GGTCTC 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 231, 589
BspACI CCGC 1 cut(s) 844
BspANI GGCC 2 cut(s) 71, 187
BspCNI CTCAG 2 cut(s) 327, 601
BspDI ATCGAT 2 cut(s) 630, 642
BspHI TCATGA 2 cut(s) 373, 787
BspLI GGNNCC 2 cut(s) 369, 558
BspPI GGATC 1 cut(s) 600
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 2 cut(s) 326, 330
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 290, 547
Bst2UI CCWGG 3 cut(s) 189, 452, 549
Bst4CI ACNGT 3 cut(s) 448, 512, 884
Bst6I CTCTTC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 460
BstDEI CTNAG 3 cut(s) 182, 335, 588
BstF5I GGATG 1 cut(s) 492
BstMAI GTCTC 1 cut(s) 108
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 3 cut(s) 189, 452, 549
BstSCI CCNGG 4 cut(s) 187, 289, 450, 547
BstSFI CTRYAG 1 cut(s) 157
BstV2I GAAGAC 2 cut(s) 289, 885
Bsu15I ATCGAT 2 cut(s) 630, 642
BsuRI GGCC 2 cut(s) 71, 187
BsuTUI ATCGAT 2 cut(s) 630, 642
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 492
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 1 cut(s) 608
CciI TCATGA 2 cut(s) 373, 787
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 70, 448, 556
ClaI ATCGAT 2 cut(s) 630, 642
Csp6I GTAC 2 cut(s) 387, 406
CspAI ACCGGT 1 cut(s) 141
CviAII CATG 3 cut(s) 48, 374, 788
CviQI GTAC 2 cut(s) 387, 406
DdeI CTNAG 3 cut(s) 182, 335, 588
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 187
Eco24I GRGCYC 2 cut(s) 231, 589
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 448, 556
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 897
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 871
EcoRII CCWGG 3 cut(s) 187, 450, 547
EcoT38I GRGCYC 2 cut(s) 231, 589
FaeI CATG 3 cut(s) 51, 377, 791
FaiI YATR 6 cut(s) 49, 375, 623, 653, 789, 862
FaqI GGGAC 1 cut(s) 542
FatI CATG 3 cut(s) 47, 373, 787
FbaI TGATCA 4 cut(s) 706, 784, 790, 826
FblI GTMKAC 2 cut(s) 160, 840
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 2 cut(s) 231, 589
FspBI CTAG 2 cut(s) 213, 573
HaeIII GGCC 2 cut(s) 71, 187
HapII CCGG 2 cut(s) 142, 290
Hin1II CATG 3 cut(s) 51, 377, 791
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HindIII AAGCTT 2 cut(s) 248, 734
HinfI GANTC 4 cut(s) 428, 457, 605, 726
HpaII CCGG 2 cut(s) 142, 290
HphI GGTGA 4 cut(s) 466, 721, 764, 896
Hpy166II GTNNAC 5 cut(s) 161, 408, 544, 755, 841
Hpy188I TCNGA 6 cut(s) 336, 427, 444, 658, 870, 877
Hpy8I GTNNAC 5 cut(s) 161, 408, 544, 755, 841
HpyAV CCTTC 3 cut(s) 23, 95, 128
HpyCH4III ACNGT 3 cut(s) 448, 512, 884
HpyCH4V TGCA 3 cut(s) 244, 480, 854
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 3 cut(s) 182, 335, 588
Hsp92II CATG 3 cut(s) 51, 377, 791
Ksp22I TGATCA 4 cut(s) 706, 784, 790, 826
LmnI GCTCC 3 cut(s) 44, 226, 373
MaeI CTAG 2 cut(s) 213, 573
MaeIII GTNAC 4 cut(s) 203, 359, 435, 512
MboII GAAGA 9 cut(s) 74, 230, 233, 294, 496, 674, 731, 752, 890
MhlI GDGCHC 2 cut(s) 231, 589
MluCI AATT 5 cut(s) 175, 677, 769, 855, 871
MlyI GAGTC 1 cut(s) 599
MnlI CCTC 7 cut(s) 132, 194, 217, 381, 595, 659, 857
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 3 cut(s) 475, 613, 798
MspI CCGG 2 cut(s) 142, 290
MspR9I CCNGG 4 cut(s) 189, 291, 452, 549
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 3 cut(s) 189, 452, 549
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 291
NlaIII CATG 3 cut(s) 51, 377, 791
NlaIV GGNNCC 2 cut(s) 369, 558
NmuCI GTSAC 3 cut(s) 359, 435, 512
NspV TTCGAA 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 613
PagI TCATGA 2 cut(s) 373, 787
PceI AGGCCT 1 cut(s) 187
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 428, 457, 726
PinAI ACCGGT 1 cut(s) 141
PleI GAGTC 1 cut(s) 599
PpsI GAGTC 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 3 cut(s) 187, 450, 547
PspGI CCWGG 3 cut(s) 187, 450, 547
PspN4I GGNNCC 2 cut(s) 369, 558
PspPI GGNCC 3 cut(s) 70, 448, 556
RsaI GTAC 2 cut(s) 388, 407
RsaNI GTAC 2 cut(s) 387, 406
RseI CAYNNNNRTG 3 cut(s) 475, 613, 798
SacI GAGCTC 1 cut(s) 231
SalI GTCGAC 1 cut(s) 839
SaqAI TTAA 1 cut(s) 195
Sau96I GGNCC 3 cut(s) 70, 448, 556
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 4 cut(s) 189, 291, 452, 549
SduI GDGCHC 2 cut(s) 231, 589
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 2 cut(s) 448, 556
SmiMI CAYNNNNRTG 3 cut(s) 475, 613, 798
Sse9I AATT 5 cut(s) 175, 677, 769, 855, 871
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 1 cut(s) 844
SspMI CTAG 2 cut(s) 213, 573
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 4 cut(s) 187, 289, 450, 547
TaaI ACNGT 3 cut(s) 448, 512, 884
TaqI TCGA 4 cut(s) 460, 630, 642, 840
TasI AATT 5 cut(s) 175, 677, 769, 855, 871
TatI WGTACW 2 cut(s) 386, 405
TfiI GAWTC 3 cut(s) 428, 457, 726
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 615
TseFI GTSAC 3 cut(s) 359, 435, 512
Tsp45I GTSAC 3 cut(s) 359, 435, 512
TspDTI ATGAA 1 cut(s) 849
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 615
VpaK11BI GGWCC 2 cut(s) 448, 556
XapI RAATTY 2 cut(s) 677, 871
XmiI GTMKAC 2 cut(s) 160, 840
XspI CTAG 2 cut(s) 213, 573
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.