MD02G1094400.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013373
Physical Location & Seq
Reverse (-)
7491531 .. 7491980
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1094400.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCTATAACAGGCACAGACTCCCAGCACCAAACCTCATCTAAACCATCCCCAGTTCCTCCACCAGTCGAAGTCCAGTGCGTAAAATGCCACTCCTGCGGGTTCACAGAGGATTGCACCCCAGCCTACATCTCCCGCGTCCGAGACCGCTACCAGGGCCGTTGGATTTGCGGGCTCTGCGTCGAGGCGGTGAAAGACGAACTGGTGCGATCAGATAGGCTCATCTCCACAGAGGAAGCCCTGAACCGCCACATCAACTTCTGCAAAAAGTTCCGGTCGCAGAAAGAAACAGAGCACCCCATCTTCGCCATGGGTCGGATTTTCCGGCGGAGCTTGGATACTCCGAGAGCCCTGAGGTCAAATTCCGGTAGTAACCTCGGGGAAGTGGAAGGGGTTCGCCGGCCGCCGCTTGTTCGATCGGGAAGTTGCTTCCCTTCTCTGTCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

150

Amino Acids

16.75

Weight (kDa)

9.2

Isoelectric Point (pI)

74.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 25 - 114 2.6e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20670
fragaria_vesca FvH4_1g08900
malus_domestica MD02G1094400.v1.1 MD15G1219300.v1.1
prunus_persica Prupe.7G196600_v2.0.a1
pyrus_communis pycom02g07470 pycom15g19460
rosa_chinensis RchiOBHm_Chr2g0095361 RchiOBHm_Chr6g0253361
rosa_laevigata RLG00000016558
rosa_multiflora Rmu_ssc0000183.1_g000036
rosa_roxburghii Rroxscaffold_2G00146440
rosa_rugosa Rorug02G0050000
rosa_samantha Rh2AG098200 Rh2BG099400 Rh2CG101400 Rh2DG099000
rosa_wichuraiana Rw2G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 138
AciI CCGC 9 cut(s) 99, 136, 148, 171, 188, 247, 328, 404, 407
AcoI YGGCCR 1 cut(s) 401
AcsI RAATTY 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 154, 315
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 2 cut(s) 157, 401
ApoI RAATTY 1 cut(s) 361
Asp700I GAANNNNTTC 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 353
BanII GRGCYC 2 cut(s) 177, 352
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 2 cut(s) 55, 308
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 155
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 444
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 2 cut(s) 404, 407
BlsI GCNGC 2 cut(s) 405, 408
Bme1390I CCNGG 1 cut(s) 155
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 155
BmrI ACTGGG 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 154, 309, 376
BsaWI WCCGGW 2 cut(s) 273, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 315
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 4 cut(s) 53, 65, 76, 207
Bse21I CCTNAGG 1 cut(s) 353
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 3 cut(s) 154, 309, 376
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 2 cut(s) 154, 315
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 4 cut(s) 53, 65, 76, 207
BseX3I CGGCCG 1 cut(s) 401
BseYI CCCAGC 2 cut(s) 24, 121
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 3 cut(s) 278, 404, 419
BshFI GGCC 2 cut(s) 159, 403
BsiEI CGRYCG 3 cut(s) 278, 404, 419
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 4 cut(s) 274, 325, 366, 400
BslI CCNNNNNNNGG 2 cut(s) 154, 315
BsmAI GTCTC 1 cut(s) 138
BsnI GGCC 2 cut(s) 159, 403
Bso31I GGTCTC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 177, 297, 352
Bsp143I GATC 2 cut(s) 209, 416
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 9 cut(s) 99, 136, 148, 171, 188, 247, 328, 404, 407
BspANI GGCC 2 cut(s) 159, 403
BspCNI CTCAG 1 cut(s) 345
BspFNI CGCG 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 4 cut(s) 53, 65, 76, 207
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 3 cut(s) 154, 309, 376
BssMI GATC 2 cut(s) 209, 416
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 155
BstC8I GCNNGC 2 cut(s) 173, 401
BstDEI CTNAG 1 cut(s) 353
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 47
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 2 cut(s) 212, 419
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 209, 416
BstMCI CGRYCG 3 cut(s) 278, 404, 419
BstMWI GCNNNNNNNGC 4 cut(s) 87, 96, 156, 177
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 153
BstUI CGCG 1 cut(s) 138
BstZI CGGCCG 1 cut(s) 401
Bsu36I CCTNAGG 1 cut(s) 353
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 159, 403
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 173, 401
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 2 cut(s) 127, 169
CviAII CATG 1 cut(s) 310
CviJI RGCY 9 cut(s) 5, 125, 159, 175, 220, 239, 333, 350, 403
CviKI_1 RGCY 9 cut(s) 5, 125, 159, 175, 220, 239, 333, 350, 403
DdeI CTNAG 1 cut(s) 353
DpnI GATC 2 cut(s) 211, 418
DpnII GATC 2 cut(s) 209, 416
EaeI YGGCCR 1 cut(s) 401
EagI CGGCCG 1 cut(s) 401
EciI GGCGGA 1 cut(s) 343
EclXI CGGCCG 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 2 cut(s) 177, 352
Eco31I GGTCTC 1 cut(s) 138
Eco52I CGGCCG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 2 cut(s) 177, 352
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 1 cut(s) 313
FaiI YATR 2 cut(s) 8, 311
FatI CATG 1 cut(s) 309
FauI CCCGC 3 cut(s) 92, 143, 164
Fnu4HI GCNGC 2 cut(s) 404, 407
FokI GGATG 1 cut(s) 34
FriOI GRGCYC 2 cut(s) 177, 352
Fsp4HI GCNGC 2 cut(s) 404, 407
FspBI CTAG 1 cut(s) 444
GluI GCNGC 2 cut(s) 404, 407
GsaI CCCAGC 2 cut(s) 28, 125
HaeIII GGCC 2 cut(s) 159, 403
HapII CCGG 4 cut(s) 274, 325, 366, 400
HgaI GACGC 2 cut(s) 127, 169
Hin1II CATG 1 cut(s) 313
HinfI GANTC 1 cut(s) 20
HpaII CCGG 4 cut(s) 274, 325, 366, 400
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 4 cut(s) 143, 214, 318, 345
Hpy188III TCNNGA 1 cut(s) 420
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 383, 444
HpyCH4V TGCA 2 cut(s) 117, 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 96, 156, 177
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 1 cut(s) 313
KroI GCCGGC 1 cut(s) 399
KroNI GCCGGC 1 cut(s) 401
Kzo9I GATC 2 cut(s) 209, 416
LmnI GCTCC 1 cut(s) 330
MaeI CTAG 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 371
MalI GATC 2 cut(s) 211, 418
MboI GATC 2 cut(s) 209, 416
MboII GAAGA 1 cut(s) 295
MhlI GDGCHC 3 cut(s) 177, 297, 352
MluCI AATT 1 cut(s) 361
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 143, 296
MnlI CCTC 7 cut(s) 46, 69, 103, 178, 226, 348, 386
MroNI GCCGGC 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 393
MspI CCGG 4 cut(s) 274, 325, 366, 400
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 87, 96, 156, 177
NaeI GCCGGC 1 cut(s) 401
NcoI CCATGG 1 cut(s) 309
NdeII GATC 2 cut(s) 209, 416
NgoMIV GCCGGC 1 cut(s) 399
NlaIII CATG 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 393
PkrI GCNGC 2 cut(s) 405, 408
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 2 cut(s) 24, 121
PspGI CCWGG 1 cut(s) 153
PspPI GGNCC 1 cut(s) 157
PvuI CGATCG 1 cut(s) 419
SatI GCNGC 2 cut(s) 404, 407
Sau3AI GATC 2 cut(s) 209, 416
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 3 cut(s) 177, 297, 352
SetI ASST 5 cut(s) 38, 335, 359, 378, 449
Sse9I AATT 1 cut(s) 361
SsiI CCGC 9 cut(s) 99, 136, 148, 171, 188, 247, 328, 404, 407
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 1 cut(s) 309
TaqI TCGA 3 cut(s) 69, 183, 415
TasI AATT 1 cut(s) 361
TauI GCSGC 2 cut(s) 406, 409
TscAI CASTG 1 cut(s) 83
TspRI CASTG 1 cut(s) 83
XapI RAATTY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.