RLG00000016558

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013373
Physical Location & Seq
Reverse (-)
7643465 .. 7644132
668 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016558

Sequence Viewer

Length: 465 bp
ATGCTCTGCTCTGCAACAATGGCTATCAGCGAGTCTCACCCCCAAGCCTCCACCAAACCAAACCCTTCTCCTCCAATGGAAGTCCACTGCGTCAAATGCGACTCCTGCGGCTTCACAGAGGATTGCACCCCCGCGTACATCGCACGCGTCCGTGAGCGCTACCAGGGACGGTGGATATGTGGGCTCTGCGTGGAGGCCGTGAAAGACGAGGCGCTCAGATCAGATAGGCTCATCTCCACCGAGGAGGCCCTGAACCGCCACATAAGCTTCTGCAAGAATTTCAGATCGTCCAGTAAGGAAAAAACAGAGCACCCTATTCTGGCCATGGGCCGGGTTTTCCGCCGGAGCTTGGATACTCCGAGGGCTCTCCGGTCCAATTCTAGCTCCTCTCTCGGTGGCGTTGAGAGGGTTGGTGGGCCGGCGCTGGTTAGGTCCGGCAGTTGCTTCCCTGCTCTCAATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

155

Amino Acids

16.89

Weight (kDa)

8.81

Isoelectric Point (pI)

70.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 29 - 119 1.4e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20670
fragaria_vesca FvH4_1g08900
malus_domestica MD02G1094400.v1.1 MD15G1219300.v1.1
prunus_persica Prupe.7G196600_v2.0.a1
pyrus_communis pycom02g07470 pycom15g19460
rosa_chinensis RchiOBHm_Chr2g0095361 RchiOBHm_Chr6g0253361
rosa_laevigata RLG00000016558
rosa_multiflora Rmu_ssc0000183.1_g000036
rosa_roxburghii Rroxscaffold_2G00146440
rosa_rugosa Rorug02G0050000
rosa_samantha Rh2AG098200 Rh2BG099400 Rh2CG101400 Rh2DG099000
rosa_wichuraiana Rw2G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 134, 147
AciI CCGC 4 cut(s) 108, 132, 256, 340
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 277
AfaI GTAC 1 cut(s) 137
AfeI AGCGCT 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 319, 330, 349
AflIII ACRYGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 4 cut(s) 267, 348, 384, 462
AluI AGCT 4 cut(s) 267, 348, 384, 462
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 39
Aor51HI AGCGCT 1 cut(s) 158
AoxI GGCC 5 cut(s) 195, 246, 321, 328, 416
ApoI RAATTY 1 cut(s) 277
AspLEI GCGC 3 cut(s) 159, 214, 424
AspS9I GGNCC 5 cut(s) 247, 328, 372, 416, 432
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 29
AvaII GGWCC 2 cut(s) 372, 432
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 2 cut(s) 186, 367
Bbv12I GWGCWC 1 cut(s) 312
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 164
BciVI GTATCC 1 cut(s) 346
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 1 cut(s) 381
BfoI RGCGCY 3 cut(s) 160, 215, 425
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
Bme1390I CCNGG 2 cut(s) 164, 332
Bme18I GGWCC 2 cut(s) 372, 432
BmgT120I GGNCC 5 cut(s) 247, 328, 372, 416, 432
BmrFI CCNGG 2 cut(s) 164, 332
BpuMI CCSGG 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 163, 240, 324, 359
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 330, 349
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 4 cut(s) 163, 240, 324, 359
BseLI CCNNNNNNNGG 3 cut(s) 319, 330, 349
BseMII CTCAG 1 cut(s) 229
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 3 cut(s) 60, 257, 376
Bsh1236I CGCG 2 cut(s) 134, 147
BshFI GGCC 5 cut(s) 197, 248, 323, 330, 418
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 5 cut(s) 331, 343, 370, 419, 435
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 319, 330, 349
BsmAI GTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 5 cut(s) 197, 248, 323, 330, 418
Bsp1286I GDGCHC 3 cut(s) 186, 312, 367
Bsp143I GATC 2 cut(s) 218, 284
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 4 cut(s) 108, 132, 256, 340
BspANI GGCC 5 cut(s) 197, 248, 323, 330, 418
BspCNI CTCAG 1 cut(s) 228
BspFNI CGCG 2 cut(s) 134, 147
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 4 cut(s) 163, 240, 324, 359
BssMI GATC 2 cut(s) 218, 284
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 145, 420
BstDEI CTNAG 1 cut(s) 215
BstDSI CCRYGG 1 cut(s) 324
BstFNI CGCG 2 cut(s) 134, 147
BstH2I RGCGCY 3 cut(s) 160, 215, 425
BstHHI GCGC 3 cut(s) 159, 214, 424
BstKTI GATC 2 cut(s) 221, 287
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 2 cut(s) 218, 284
BstMWI GCNNNNNNNGC 5 cut(s) 20, 96, 105, 140, 264
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 162, 330
BstUI CGCG 2 cut(s) 134, 147
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 5 cut(s) 197, 248, 323, 330, 418
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 145, 420
CfoI GCGC 3 cut(s) 159, 214, 424
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 5 cut(s) 247, 328, 372, 416, 432
CseI GACGC 2 cut(s) 79, 136
Csp6I GTAC 1 cut(s) 136
CviAII CATG 1 cut(s) 325
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 215
DpnI GATC 2 cut(s) 220, 286
DpnII GATC 2 cut(s) 218, 284
EaeI YGGCCR 1 cut(s) 321
EciI GGCGGA 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 2 cut(s) 186, 367
Eco47I GGWCC 2 cut(s) 372, 432
Eco47III AGCGCT 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 186, 367
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 1 cut(s) 328
FaiI YATR 3 cut(s) 178, 263, 326
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 324
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 109
FriOI GRGCYC 2 cut(s) 186, 367
Fsp4HI GCNGC 1 cut(s) 109
FspBI CTAG 1 cut(s) 381
GlaI GCGC 3 cut(s) 158, 213, 423
GluI GCNGC 1 cut(s) 109
HaeII RGCGCY 3 cut(s) 160, 215, 425
HaeIII GGCC 5 cut(s) 197, 248, 323, 330, 418
HapII CCGG 5 cut(s) 331, 343, 370, 419, 435
HgaI GACGC 2 cut(s) 79, 136
HhaI GCGC 3 cut(s) 159, 214, 424
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 3 cut(s) 157, 212, 422
HinP1I GCGC 3 cut(s) 157, 212, 422
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 2 cut(s) 32, 101
HpaII CCGG 5 cut(s) 331, 343, 370, 419, 435
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 4 cut(s) 218, 223, 284, 360
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 14, 126, 273
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 96, 105, 140, 264
HpyF3I CTNAG 1 cut(s) 215
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 3 cut(s) 157, 212, 422
KroI GCCGGC 1 cut(s) 418
KroNI GCCGGC 1 cut(s) 420
Kzo9I GATC 2 cut(s) 218, 284
LmnI GCTCC 2 cut(s) 345, 389
MaeI CTAG 1 cut(s) 381
MalI GATC 2 cut(s) 220, 286
MboI GATC 2 cut(s) 218, 284
MhlI GDGCHC 3 cut(s) 186, 312, 367
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 2 cut(s) 277, 376
MluI ACGCGT 1 cut(s) 145
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 41, 95
Mox20I TGGCCA 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 323
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 5 cut(s) 331, 343, 370, 419, 435
MspR9I CCNGG 2 cut(s) 164, 332
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 134, 147
MwoI GCNNNNNNNGC 5 cut(s) 20, 96, 105, 140, 264
NaeI GCCGGC 1 cut(s) 420
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 324
NdeII GATC 2 cut(s) 218, 284
NgoMIV GCCGGC 1 cut(s) 418
NlaIII CATG 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 420
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 2 cut(s) 40, 95
PpsI GAGTC 2 cut(s) 40, 95
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspPI GGNCC 5 cut(s) 247, 328, 372, 416, 432
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 2 cut(s) 218, 284
Sau96I GGNCC 5 cut(s) 247, 328, 372, 416, 432
SchI GAGTC 2 cut(s) 41, 95
ScrFI CCNGG 2 cut(s) 164, 332
SduI GDGCHC 3 cut(s) 186, 312, 367
SetI ASST 5 cut(s) 269, 350, 386, 434, 464
SinI GGWCC 2 cut(s) 372, 432
Sse9I AATT 2 cut(s) 277, 376
SsiI CCGC 4 cut(s) 108, 132, 256, 340
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 2 cut(s) 162, 330
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 171
TasI AATT 2 cut(s) 277, 376
TauI GCSGC 1 cut(s) 111
TscAI CASTG 1 cut(s) 92
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 372, 432
XapI RAATTY 1 cut(s) 277
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.