Rorug02G0050000

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013373
Physical Location & Seq
Forward (+)
3864793 .. 3867875
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0050000.1

Sequence Viewer

Length: 1863 bp
ATGCTGGACGGACTGCTCGGCCGTGGTTTCGGCGCCAAGTGTAAATCGCTGATAAAATTGACGAAAACTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTTTGAAGAAAGATATAGCTGATCTACTCAACCATGGCCTCGAAATTAATGCTTTCGGAAGGGCTGAGGGACTTACAGTGGAGGTGATACTTTCGGCGTGTTACGATTTCGTTGAGCGATGCTGTGATTTGGTGTTGAAGCATCTTTCAGTCATGCAGAAACAAAGTGAATGCCCCGAGGACTGTAAGGAAGCTGTTGCATCACTGATGGTTGCTGCGGCAAGATTTTCTGATTTACCAGAGTTGCGTGACATTAGGCAACTATTTCAAGAGAGATACGCGAATTCTTTGGATTACTGTGCGGATCAAAAGTTTGTTGAGAACATAGCTTCCAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATTAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGCAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCTGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAAGGAGGTTATAGCCTTGAAGAAGGATGAGCTCGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTCAAAGGCGGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGGCATTATGAAGCAGAGAATAGCGTTCGTAAAAGAGACGACCCCAACACTATTCCTCATGTAAAGCCAAATGTTGCTGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAGCTAATTTCGGTCATGATTGTTCTCAAGGTACCGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCTTTGCTGATAATGTGTTGGAAAGGGTACAACTAGGTCGTGATCATGAGAGGCGTGCTACTGCGAAAGTTGATAGTTATGATCATGAGAACGAGTATGTTCATCAGGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAATGAGCAAACCAAGAAGCAGGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAGCTGTTGTTTGATGATGACCACCACCACAGGAAAGATGATGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATCTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCCAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCATGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

620

Amino Acids

69.83

Weight (kDa)

9.29

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 170 2.1e-42 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20670
fragaria_vesca FvH4_1g08900
malus_domestica MD02G1094400.v1.1 MD15G1219300.v1.1
prunus_persica Prupe.7G196600_v2.0.a1
pyrus_communis pycom02g07470 pycom15g19460
rosa_chinensis RchiOBHm_Chr2g0095361 RchiOBHm_Chr6g0253361
rosa_laevigata RLG00000016558
rosa_multiflora Rmu_ssc0000183.1_g000036
rosa_roxburghii Rroxscaffold_2G00146440
rosa_rugosa Rorug02G0050000
rosa_samantha Rh2AG098200 Rh2BG099400 Rh2CG101400 Rh2DG099000
rosa_wichuraiana Rw2G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1196
AccB1I GGYRCC 2 cut(s) 32, 1196
AccII CGCG 3 cut(s) 101, 392, 1405
AciI CCGC 4 cut(s) 329, 413, 804, 1757
AclWI GGATC 5 cut(s) 80, 423, 680, 1208, 1221
AcoI YGGCCR 2 cut(s) 19, 697
AcsI RAATTY 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 33, 1736
AfaI GTAC 4 cut(s) 639, 1198, 1263, 1365
AfiI CCNNNNNNNGG 1 cut(s) 695
AflII CTTAAG 1 cut(s) 1849
AgsI TTSAA 9 cut(s) 118, 250, 380, 473, 504, 530, 736, 903, 1093
AhdI GACNNNNNGTC 1 cut(s) 664
AjiI CACGTC 1 cut(s) 1499
AjnI CCWGG 2 cut(s) 849, 1609
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 9 cut(s) 131, 305, 440, 748, 1169, 1352, 1448, 1510, 1790
AluI AGCT 9 cut(s) 131, 305, 440, 748, 1169, 1352, 1448, 1510, 1790
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 6 cut(s) 673, 798, 821, 978, 1384, 1727
AlwI GGATC 5 cut(s) 80, 423, 680, 1208, 1221
Ama87I CYCGRG 2 cut(s) 287, 930
AoxI GGCC 7 cut(s) 19, 148, 522, 697, 832, 1111, 1501
ApeKI GCWGC 7 cut(s) 326, 1220, 1448, 1507, 1573, 1832, 1844
ApoI RAATTY 1 cut(s) 394
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 1097
Asp718I GGTACC 1 cut(s) 1196
AspLEI GCGC 2 cut(s) 35, 1407
AspS9I GGNCC 4 cut(s) 832, 1028, 1724, 1779
AsuHPI GGTGA 2 cut(s) 208, 1445
AvaI CYCGRG 2 cut(s) 287, 930
AvaII GGWCC 3 cut(s) 1028, 1724, 1779
BalI TGGCCA 1 cut(s) 699
BamHI GGATCC 1 cut(s) 1213
BanI GGYRCC 2 cut(s) 32, 1196
BanII GRGCYC 1 cut(s) 750
BauI CACGAG 1 cut(s) 1752
Bbv12I GWGCWC 1 cut(s) 750
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 7 cut(s) 313, 1232, 1435, 1519, 1560, 1819, 1856
BccI CCATC 6 cut(s) 313, 669, 1040, 1603, 1735, 1769
BceAI ACGGC 1 cut(s) 6
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 851, 1611
BclI TGATCA 3 cut(s) 754, 1276, 1315
BcoDI GTCTC 6 cut(s) 673, 798, 821, 978, 1384, 1727
BfaI CTAG 3 cut(s) 762, 1268, 1383
BfmI CTRYAG 2 cut(s) 1218, 1505
BfoI RGCGCY 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 1849
BisI GCNGC 9 cut(s) 327, 330, 1221, 1449, 1508, 1574, 1758, 1833, 1845
BlsI GCNGC 9 cut(s) 328, 331, 1222, 1450, 1509, 1575, 1759, 1834, 1846
BmcAI AGTACT 1 cut(s) 639
Bme1390I CCNGG 2 cut(s) 851, 1611
Bme18I GGWCC 3 cut(s) 1028, 1724, 1779
BmeRI GACNNNNNGTC 1 cut(s) 664
BmeT110I CYCGRG 2 cut(s) 287, 930
BmgBI CACGTC 1 cut(s) 1499
BmgT120I GGNCC 4 cut(s) 832, 1028, 1724, 1779
BmiI GGNNCC 5 cut(s) 34, 1198, 1215, 1608, 1780
BmrFI CCNGG 2 cut(s) 851, 1611
BmsI GCATC 8 cut(s) 221, 262, 320, 849, 883, 1212, 1411, 1810
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 3 cut(s) 803, 1176, 1381
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 33, 1736
BsaI GGTCTC 1 cut(s) 1384
BsaJI CCNNGG 6 cut(s) 22, 145, 288, 519, 835, 1610
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse118I RCCGGY 1 cut(s) 1199
Bse1I ACTGG 1 cut(s) 908
Bse3DI GCAATG 1 cut(s) 1458
BseBI CCWGG 2 cut(s) 851, 1611
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 6 cut(s) 22, 145, 288, 519, 835, 1610
BseGI GGATG 5 cut(s) 748, 898, 1348, 1492, 1801
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 1 cut(s) 1458
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 908
BseRI GAGGAG 3 cut(s) 1110, 1399, 1685
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 7 cut(s) 313, 1232, 1435, 1519, 1560, 1819, 1856
BseYI CCCAGC 2 cut(s) 1664, 1786
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 3 cut(s) 101, 392, 1405
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 7 cut(s) 21, 150, 524, 699, 834, 1113, 1503
BshNI GGYRCC 2 cut(s) 32, 1196
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 750
BsiHKCI CYCGRG 2 cut(s) 287, 930
BsiSI CCGG 1 cut(s) 1200
BslFI GGGAC 2 cut(s) 195, 527
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 6 cut(s) 673, 798, 821, 978, 1384, 1727
BsmBI CGTCTC 2 cut(s) 978, 1727
BsmFI GGGAC 2 cut(s) 195, 527
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 7 cut(s) 21, 150, 524, 699, 834, 1113, 1503
Bso31I GGTCTC 1 cut(s) 1384
BsoBI CYCGRG 2 cut(s) 287, 930
Bsp1286I GDGCHC 1 cut(s) 750
Bsp19I CCATGG 2 cut(s) 145, 835
BspACI CCGC 4 cut(s) 329, 413, 804, 1757
BspANI GGCC 7 cut(s) 21, 150, 524, 699, 834, 1113, 1503
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 3 cut(s) 101, 392, 1405
BspHI TCATGA 4 cut(s) 1180, 1279, 1318, 1711
BspLI GGNNCC 5 cut(s) 34, 1198, 1215, 1608, 1780
BspMAI CTGCAG 2 cut(s) 1222, 1509
BspPI GGATC 5 cut(s) 80, 423, 680, 1208, 1221
BspT107I GGYRCC 2 cut(s) 32, 1196
BspTI CTTAAG 1 cut(s) 1849
BspTNI GGTCTC 1 cut(s) 1384
BsrDI GCAATG 1 cut(s) 1458
BsrFI RCCGGY 1 cut(s) 1199
BsrI ACTGG 1 cut(s) 908
BssAI RCCGGY 1 cut(s) 1199
BssECI CCNNGG 6 cut(s) 22, 145, 288, 519, 835, 1610
BssNI GRCGYC 2 cut(s) 33, 1736
BssSI CACGAG 1 cut(s) 1752
BssT1I CCWWGG 3 cut(s) 145, 519, 835
Bst2BI CACGAG 1 cut(s) 1752
Bst2UI CCWGG 2 cut(s) 851, 1611
Bst4CI ACNGT 4 cut(s) 190, 296, 410, 1433
Bst6I CTCTTC 2 cut(s) 86, 1545
BstACI GRCGYC 2 cut(s) 33, 1736
BstAFI CTTAAG 1 cut(s) 1849
BstC8I GCNNGC 5 cut(s) 1290, 1426, 1505, 1648, 1662
BstDEI CTNAG 2 cut(s) 177, 1691
BstDSI CCRYGG 3 cut(s) 22, 145, 835
BstF5I GGATG 5 cut(s) 748, 898, 1348, 1492, 1801
BstFNI CGCG 3 cut(s) 101, 392, 1405
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 1407
BstMAI GTCTC 6 cut(s) 673, 798, 821, 978, 1384, 1727
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 1757, 1841
BstNI CCWGG 2 cut(s) 851, 1611
BstNSI RCATGY 1 cut(s) 1799
BstSCI CCNGG 2 cut(s) 849, 1609
BstSFI CTRYAG 2 cut(s) 1218, 1505
BstUI CGCG 3 cut(s) 101, 392, 1405
BstV1I GCAGC 7 cut(s) 313, 1232, 1435, 1519, 1560, 1819, 1856
BstX2I RGATCY 1 cut(s) 1213
BstXI CCANNNNNNTGG 1 cut(s) 1775
BstYI RGATCY 1 cut(s) 1213
BstZI CGGCCG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 7 cut(s) 21, 150, 524, 699, 834, 1113, 1503
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 3 cut(s) 22, 145, 835
BtgZI GCGATG 1 cut(s) 244
BtrI CACGTC 1 cut(s) 1499
BtsCI GGATG 5 cut(s) 748, 898, 1348, 1492, 1801
BtsIMutI CAGTG 2 cut(s) 195, 314
Cac8I GCNNGC 5 cut(s) 1290, 1426, 1505, 1648, 1662
CciI TCATGA 4 cut(s) 1180, 1279, 1318, 1711
CfoI GCGC 2 cut(s) 35, 1407
Cfr10I RCCGGY 1 cut(s) 1199
Cfr13I GGNCC 4 cut(s) 832, 1028, 1724, 1779
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 112, 1744
Csp6I GTAC 4 cut(s) 638, 1197, 1262, 1364
CviQI GTAC 4 cut(s) 638, 1197, 1262, 1364
DdeI CTNAG 2 cut(s) 177, 1691
DinI GGCGCC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 2 cut(s) 19, 697
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 86, 1545
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 2 cut(s) 86, 1545
Ecl136II GAGCTC 1 cut(s) 748
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 145, 519, 835
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 1384
Eco47I GGWCC 3 cut(s) 1028, 1724, 1779
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 748
Eco88I CYCGRG 2 cut(s) 287, 930
EcoICRI GAGCTC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 849, 1609
EcoT14I CCWWGG 3 cut(s) 145, 519, 835
EcoT38I GRGCYC 1 cut(s) 750
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 3 cut(s) 145, 519, 835
Esp3I CGTCTC 2 cut(s) 978, 1727
FalI AAGNNNNNCTT 4 cut(s) 1220, 1252, 1684, 1716
FaqI GGGAC 2 cut(s) 195, 527
FauI CCCGC 1 cut(s) 797
FbaI TGATCA 3 cut(s) 754, 1276, 1315
Fnu4HI GCNGC 9 cut(s) 327, 330, 1221, 1449, 1508, 1574, 1758, 1833, 1845
FokI GGATG 5 cut(s) 755, 905, 1355, 1499, 1788
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 9 cut(s) 327, 330, 1221, 1449, 1508, 1574, 1758, 1833, 1845
FspBI CTAG 3 cut(s) 762, 1268, 1383
GlaI GCGC 2 cut(s) 34, 1406
GluI GCNGC 9 cut(s) 327, 330, 1221, 1449, 1508, 1574, 1758, 1833, 1845
GsaI CCCAGC 2 cut(s) 1668, 1790
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 7 cut(s) 21, 150, 524, 699, 834, 1113, 1503
HapII CCGG 1 cut(s) 1200
HgaI GACGC 2 cut(s) 112, 1744
HhaI GCGC 2 cut(s) 35, 1407
Hin1I GRCGYC 2 cut(s) 33, 1736
Hin6I GCGC 2 cut(s) 33, 1405
HinP1I GCGC 2 cut(s) 33, 1405
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HinfI GANTC 5 cut(s) 82, 515, 1055, 1490, 1673
HpaII CCGG 1 cut(s) 1200
HphI GGTGA 2 cut(s) 208, 1445
Hpy166II GTNNAC 1 cut(s) 669
Hpy188I TCNGA 8 cut(s) 72, 87, 170, 343, 759, 1145, 1678, 1732
Hpy8I GTNNAC 1 cut(s) 669
Hpy99I CGWCG 1 cut(s) 106
HpyCH4III ACNGT 4 cut(s) 190, 296, 410, 1433
HpyCH4IV ACGT 1 cut(s) 1498
HpyCH4V TGCA 9 cut(s) 268, 311, 635, 1220, 1451, 1507, 1646, 1801, 1844
HpyF10VI GCNNNNNNNGC 2 cut(s) 1757, 1841
HpyF3I CTNAG 2 cut(s) 177, 1691
HpySE526I ACGT 1 cut(s) 1498
Hsp92I GRCGYC 2 cut(s) 33, 1736
HspAI GCGC 2 cut(s) 33, 1405
KasI GGCGCC 1 cut(s) 32
KpnI GGTACC 1 cut(s) 1200
Ksp22I TGATCA 3 cut(s) 754, 1276, 1315
LmnI GCTCC 5 cut(s) 901, 941, 1412, 1445, 1606
Lsp1109I GCAGC 7 cut(s) 313, 1232, 1435, 1519, 1560, 1819, 1856
LweI GCATC 8 cut(s) 221, 262, 320, 849, 883, 1212, 1411, 1810
MaeI CTAG 3 cut(s) 762, 1268, 1383
MaeII ACGT 1 cut(s) 1498
MaeIII GTNAC 3 cut(s) 212, 359, 595
MboII GAAGA 9 cut(s) 103, 130, 748, 918, 1085, 1448, 1562, 1569, 1635
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 1 cut(s) 750
MlsI TGGCCA 1 cut(s) 699
MluCI AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1088, 1171, 1560
MluNI TGGCCA 1 cut(s) 699
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 509, 1499, 1682
MmeI TCCRAC 4 cut(s) 1128, 1233, 1792, 1806
Mox20I TGGCCA 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 1097
MscI TGGCCA 1 cut(s) 699
MseI TTAA 2 cut(s) 159, 1850
MslI CAYNNNNRTG 3 cut(s) 876, 1208, 1341
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 1510
MspCI CTTAAG 1 cut(s) 1849
MspI CCGG 1 cut(s) 1200
MspR9I CCNGG 2 cut(s) 851, 1611
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 2 cut(s) 851, 1611
MvnI CGCG 3 cut(s) 101, 392, 1405
MwoI GCNNNNNNNGC 2 cut(s) 1757, 1841
NarI GGCGCC 1 cut(s) 33
NcoI CCATGG 2 cut(s) 145, 835
NlaIV GGNNCC 5 cut(s) 34, 1198, 1215, 1608, 1780
NmeAIII GCCGAG 1 cut(s) 1771
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 1799
PagI TCATGA 4 cut(s) 1180, 1279, 1318, 1711
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 2 cut(s) 82, 1055
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 9 cut(s) 328, 331, 1222, 1450, 1509, 1575, 1759, 1834, 1846
PleI GAGTC 3 cut(s) 509, 1498, 1681
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 3 cut(s) 509, 1498, 1681
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 849, 1609
PspFI CCCAGC 2 cut(s) 1664, 1786
PspGI CCWGG 2 cut(s) 849, 1609
PspN4I GGNNCC 5 cut(s) 34, 1198, 1215, 1608, 1780
PspPI GGNCC 4 cut(s) 832, 1028, 1724, 1779
PstI CTGCAG 2 cut(s) 1222, 1509
PsuI RGATCY 1 cut(s) 1213
PvuII CAGCTG 1 cut(s) 1510
RsaI GTAC 4 cut(s) 639, 1198, 1263, 1365
RsaNI GTAC 4 cut(s) 638, 1197, 1262, 1364
RseI CAYNNNNRTG 3 cut(s) 876, 1208, 1341
SacI GAGCTC 1 cut(s) 750
SaqAI TTAA 2 cut(s) 159, 1850
SatI GCNGC 9 cut(s) 327, 330, 1221, 1449, 1508, 1574, 1758, 1833, 1845
Sau96I GGNCC 4 cut(s) 832, 1028, 1724, 1779
ScaI AGTACT 1 cut(s) 639
SchI GAGTC 3 cut(s) 509, 1499, 1682
ScrFI CCNGG 2 cut(s) 851, 1611
SduI GDGCHC 1 cut(s) 750
SfaNI GCATC 8 cut(s) 221, 262, 320, 849, 883, 1212, 1411, 1810
SfcI CTRYAG 2 cut(s) 1218, 1505
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 3 cut(s) 1028, 1724, 1779
SmiMI CAYNNNNRTG 3 cut(s) 876, 1208, 1341
SmlI CTYRAG 4 cut(s) 818, 1191, 1396, 1849
SmoI CTYRAG 4 cut(s) 818, 1191, 1396, 1849
Sse9I AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1088, 1171, 1560
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 4 cut(s) 329, 413, 804, 1757
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 762, 1268, 1383
SstI GAGCTC 1 cut(s) 750
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 2 cut(s) 849, 1609
StyI CCWWGG 3 cut(s) 145, 519, 835
TaaI ACNGT 4 cut(s) 190, 296, 410, 1433
TaiI ACGT 1 cut(s) 1501
TaqI TCGA 3 cut(s) 75, 153, 750
TaqII GACCGA 1 cut(s) 1166
TasI AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1088, 1171, 1560
TatI WGTACW 1 cut(s) 637
TauI GCSGC 2 cut(s) 332, 1760
TfiI GAWTC 2 cut(s) 82, 1055
Tru1I TTAA 2 cut(s) 159, 1850
Tru9I TTAA 2 cut(s) 159, 1850
TscAI CASTG 2 cut(s) 195, 321
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 7 cut(s) 326, 1220, 1448, 1507, 1573, 1832, 1844
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 8 cut(s) 494, 894, 972, 1179, 1325, 1563, 1568, 1728
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 195, 321
Vha464I CTTAAG 1 cut(s) 1849
VpaK11BI GGWCC 3 cut(s) 1028, 1724, 1779
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 1 cut(s) 394
XbaI TCTAGA 1 cut(s) 1382
XceI RCATGY 1 cut(s) 1799
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 3 cut(s) 762, 1268, 1383
ZrmI AGTACT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.