MD02G1096900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013587
Physical Location & Seq
Forward (+)
7663442 .. 7665603
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1096900.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGGGACGCGAAACGACGTTGTGGAGATTGATAGCTTGGAGGAGGGGTTACTTTCAGAAACTGCAACCGAAGCTGAAGGTGAAGAACCAGTTCTGTATTCAGCTTCGTTTCAGGAAAAAGAGGAGAATTTTGTGAAGTACCAAACTGCACGATGGCTTCTCTACTCTGGGCTTTTGGTACTGGCATGGGGGATTGGGATTTTTATGCTGCTTTATCTGCCCGTCCGCCGCTACATTCTGCGAAAGGATATTCGATCGCGGAAGCTCTATCTCACTCCCAATGCCATTGTCTACAAGTATGCAAAGCCGGTTCCATTTCCAATTTTCGGGGTGTTGAAGAAAGAGAAGCATTTTTTGTTGCCTTCAGTAGCGGATGTTGTGATCGAGCAAGGATATCTGCAGTCTCTCTTCGGTGTCTACTCACTTAGAATAGAACATGTTGGTGTCAGACGGCCGGCAAGCGATGATGTTCAAATTCATGGCATTGCGAATCCCAGTGCTTTCAGGAAGGCAGTCCTCATGCGCCTTTCAAGCATGACTAATGAGGTCTTCTCTAGACAAATTTCCACACTTGAAGATATTCCAAATTTGAAGCTTCAGGGGTCCCCGTCAAAGTCTCTCAGGTATGGGGATCTAGTGCTACTACAAAAACTTGAGGAAGTTGGAAGTTCTGTAAAGAGGGTTCAAACTATACTTGAGGAGCAACACCCTCAAACACCAGAACCTACAGATGGAAGCGCACGAAGAGTGTTAAATTTGATATGCAGAAGGTGGGAAATTTCATGTCAGGCCTTTGTTCGTACAAGTCGCACACCACTACCATGTTATTATCACCTATCTCGTCTGCCAAATCCAGGATTACAACCCCGCAAACGTTATACATTCTTCCTACATTTCTGCTGGTGCCCACTAACAAGGCCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

35.38

Weight (kDa)

9.23

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 82 - 180 6.3e-48 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013587)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09130
malus_domestica MD02G1096900.v1.1 MD15G1221700.v1.1
prunus_persica Prupe.7G194000_v2.0.a1
pyrus_communis pycom02g07690 pycom15g19620
rosa_chinensis RchiOBHm_Chr2g0095521
rosa_laevigata RLG00000016575
rosa_multiflora Rmu_sc0002541.1_g000031
rosa_roxburghii Rroxscaffold_2G00146230
rosa_rugosa Rorug02G0052100 Rorug02G0471700
rosa_samantha Rh2AG099900 Rh2BG101300 Rh2CG103500 Rh2DG100800
rosa_wichuraiana Rw2G007580 Rw7G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 903
AccI GTMKAC 2 cut(s) 291, 417
AccII CGCG 2 cut(s) 10, 259
AciI CCGC 5 cut(s) 226, 229, 259, 371, 868
AclI AACGTT 1 cut(s) 874
AclWI GGATC 1 cut(s) 639
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 6 cut(s) 127, 474, 561, 586, 754, 777
AcuI CTGAAG 3 cut(s) 96, 348, 581
AfaI GTAC 3 cut(s) 140, 180, 802
AfiI CCNNNNNNNGG 3 cut(s) 326, 731, 854
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 6 cut(s) 337, 473, 531, 575, 592, 686
AjnI CCWGG 1 cut(s) 853
AluBI AGCT 5 cut(s) 36, 74, 104, 265, 595
AluI AGCT 5 cut(s) 36, 74, 104, 265, 595
Alw26I GTCTC 2 cut(s) 408, 621
AlwI GGATC 1 cut(s) 639
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 3 cut(s) 452, 789, 917
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 6 cut(s) 127, 474, 561, 586, 754, 777
Asp700I GAANNNNTTC 2 cut(s) 90, 579
AspLEI GCGC 2 cut(s) 525, 740
AspS9I GGNCC 2 cut(s) 603, 918
AsuHPI GGTGA 2 cut(s) 92, 824
AvaII GGWCC 1 cut(s) 603
BaeGI GKGCMC 1 cut(s) 908
BanI GGYRCC 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 147, 725
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 1 cut(s) 855
BcoDI GTCTC 2 cut(s) 408, 621
BfaI CTAG 2 cut(s) 555, 635
BfmI CTRYAG 2 cut(s) 398, 726
BisI GCNGC 2 cut(s) 209, 229
BlsI GCNGC 2 cut(s) 210, 230
Bme1390I CCNGG 1 cut(s) 855
Bme18I GGWCC 1 cut(s) 603
BmgT120I GGNCC 2 cut(s) 603, 918
BmiI GGNNCC 4 cut(s) 312, 604, 605, 905
BmrFI CCNGG 1 cut(s) 855
BmrI ACTGGG 1 cut(s) 489
BmuI ACTGGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 541
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpuEI CTTGAG 2 cut(s) 674, 716
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 731, 854
Bse118I RCCGGY 2 cut(s) 307, 454
Bse1I ACTGG 3 cut(s) 89, 186, 495
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 855
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 3 cut(s) 326, 731, 854
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 634
BseNI ACTGG 3 cut(s) 89, 186, 495
BseRI GAGGAG 3 cut(s) 56, 137, 713
BseSI GKGCMC 1 cut(s) 908
BseX3I CGGCCG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 132
Bsh1236I CGCG 2 cut(s) 10, 259
Bsh1285I CGRYCG 2 cut(s) 257, 455
BshFI GGCC 3 cut(s) 454, 791, 919
BshNI GGYRCC 1 cut(s) 903
BsiEI CGRYCG 2 cut(s) 257, 455
BsiSI CCGG 2 cut(s) 308, 455
BslFI GGGAC 2 cut(s) 19, 589
BslI CCNNNNNNNGG 3 cut(s) 326, 731, 854
BsmAI GTCTC 2 cut(s) 408, 621
BsmFI GGGAC 2 cut(s) 19, 589
BsnI GGCC 3 cut(s) 454, 791, 919
Bsp1286I GDGCHC 1 cut(s) 908
Bsp143I GATC 3 cut(s) 254, 381, 631
BspACI CCGC 5 cut(s) 226, 229, 259, 371, 868
BspANI GGCC 3 cut(s) 454, 791, 919
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 2 cut(s) 10, 259
BspLI GGNNCC 4 cut(s) 312, 604, 605, 905
BspMAI CTGCAG 1 cut(s) 402
BspPI GGATC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 903
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 2 cut(s) 307, 454
BsrI ACTGG 3 cut(s) 89, 186, 495
BssAI RCCGGY 2 cut(s) 307, 454
BssMI GATC 3 cut(s) 254, 381, 631
Bst2UI CCWGG 1 cut(s) 855
Bst6I CTCTTC 2 cut(s) 413, 739
BstC8I GCNNGC 2 cut(s) 456, 460
BstDEI CTNAG 2 cut(s) 425, 620
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 2 cut(s) 10, 259
BstHHI GCGC 2 cut(s) 525, 740
BstKTI GATC 3 cut(s) 257, 384, 634
BstMAI GTCTC 2 cut(s) 408, 621
BstMBI GATC 3 cut(s) 254, 381, 631
BstMCI CGRYCG 2 cut(s) 257, 455
BstMWI GCNNNNNNNGC 3 cut(s) 71, 217, 531
BstNI CCWGG 1 cut(s) 855
BstNSI RCATGY 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 853
BstSFI CTRYAG 2 cut(s) 398, 726
BstSLI GKGCMC 1 cut(s) 908
BstUI CGCG 2 cut(s) 10, 259
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BstZI CGGCCG 1 cut(s) 452
BsuRI GGCC 3 cut(s) 454, 791, 919
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 2 cut(s) 456, 460
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 2 cut(s) 525, 740
Cfr10I RCCGGY 2 cut(s) 307, 454
Cfr13I GGNCC 2 cut(s) 603, 918
CseI GACGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 139, 179, 801
CviAII CATG 7 cut(s) 186, 437, 479, 520, 535, 783, 822
CviQI GTAC 3 cut(s) 139, 179, 801
DdeI CTNAG 2 cut(s) 425, 620
DpnI GATC 3 cut(s) 256, 383, 633
DpnII GATC 3 cut(s) 254, 381, 631
EaeI YGGCCR 1 cut(s) 452
EagI CGGCCG 1 cut(s) 452
Eam1104I CTCTTC 2 cut(s) 413, 739
EarI CTCTTC 2 cut(s) 413, 739
EciI GGCGGA 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 791
Eco32I GATATC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 603
Eco52I CGGCCG 1 cut(s) 452
Eco57I CTGAAG 3 cut(s) 96, 348, 581
EcoO109I RGGNCCY 2 cut(s) 603, 918
EcoRII CCWGG 1 cut(s) 853
EcoRV GATATC 1 cut(s) 395
FaeI CATG 7 cut(s) 189, 440, 482, 523, 538, 786, 825
FaqI GGGAC 2 cut(s) 19, 589
FatI CATG 7 cut(s) 185, 436, 478, 519, 534, 782, 821
FauI CCCGC 1 cut(s) 875
FblI GTMKAC 2 cut(s) 291, 417
Fnu4HI GCNGC 2 cut(s) 209, 229
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 209, 229
FspBI CTAG 2 cut(s) 555, 635
GlaI GCGC 2 cut(s) 524, 739
GluI GCNGC 2 cut(s) 209, 229
HaeIII GGCC 3 cut(s) 454, 791, 919
HapII CCGG 2 cut(s) 308, 455
HgaI GACGC 1 cut(s) 16
HhaI GCGC 2 cut(s) 525, 740
Hin1II CATG 7 cut(s) 189, 440, 482, 523, 538, 786, 825
Hin6I GCGC 2 cut(s) 523, 738
HinP1I GCGC 2 cut(s) 523, 738
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 1 cut(s) 490
HpaII CCGG 2 cut(s) 308, 455
HphI GGTGA 2 cut(s) 92, 824
Hpy166II GTNNAC 2 cut(s) 292, 418
Hpy188I TCNGA 2 cut(s) 58, 449
Hpy188III TCNNGA 3 cut(s) 113, 505, 555
Hpy8I GTNNAC 2 cut(s) 292, 418
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 71, 372, 502, 762
HpyCH4IV ACGT 2 cut(s) 18, 874
HpyCH4V TGCA 5 cut(s) 65, 149, 302, 400, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 217, 531
HpyF3I CTNAG 2 cut(s) 425, 620
HpySE526I ACGT 2 cut(s) 18, 874
Hsp92II CATG 7 cut(s) 189, 440, 482, 523, 538, 786, 825
HspAI GCGC 2 cut(s) 523, 738
KflI GGGWCCC 1 cut(s) 603
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 254, 381, 631
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 1 cut(s) 195
MaeI CTAG 2 cut(s) 555, 635
MaeII ACGT 2 cut(s) 18, 874
MaeIII GTNAC 1 cut(s) 48
MalI GATC 3 cut(s) 256, 383, 633
MboI GATC 3 cut(s) 254, 381, 631
MboII GAAGA 7 cut(s) 95, 349, 400, 541, 587, 756, 877
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 908
MluCI AATT 7 cut(s) 127, 321, 474, 561, 586, 754, 777
MmeI TCCRAC 1 cut(s) 643
MnlI CCTC 9 cut(s) 34, 37, 115, 527, 538, 649, 672, 691, 720
MroNI GCCGGC 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 90, 579
MseI TTAA 1 cut(s) 752
MslI CAYNNNNRTG 2 cut(s) 441, 820
MspI CCGG 2 cut(s) 308, 455
MspR9I CCNGG 1 cut(s) 855
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 2 cut(s) 10, 259
MwoI GCNNNNNNNGC 3 cut(s) 71, 217, 531
NaeI GCCGGC 1 cut(s) 456
NdeII GATC 3 cut(s) 254, 381, 631
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 7 cut(s) 189, 440, 482, 523, 538, 786, 825
NlaIV GGNNCC 4 cut(s) 312, 604, 605, 905
NspI RCATGY 1 cut(s) 440
PceI AGGCCT 1 cut(s) 791
PciI ACATGT 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 456
PdmI GAANNNNTTC 2 cut(s) 90, 579
PfeI GAWTC 1 cut(s) 490
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 2 cut(s) 210, 230
Ple19I CGATCG 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 603
PscI ACATGT 1 cut(s) 436
Psp1406I AACGTT 1 cut(s) 874
Psp5II RGGWCCY 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 853
PspGI CCWGG 1 cut(s) 853
PspN4I GGNNCC 4 cut(s) 312, 604, 605, 905
PspPI GGNCC 2 cut(s) 603, 918
PspPPI RGGWCCY 1 cut(s) 603
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstI CTGCAG 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 631
PvuI CGATCG 1 cut(s) 257
RsaI GTAC 3 cut(s) 140, 180, 802
RsaNI GTAC 3 cut(s) 139, 179, 801
RseI CAYNNNNRTG 2 cut(s) 441, 820
SaqAI TTAA 1 cut(s) 752
SatI GCNGC 2 cut(s) 209, 229
Sau3AI GATC 3 cut(s) 254, 381, 631
Sau96I GGNCC 2 cut(s) 603, 918
ScrFI CCNGG 1 cut(s) 855
SduI GDGCHC 1 cut(s) 908
SfcI CTRYAG 2 cut(s) 398, 726
SinI GGWCC 1 cut(s) 603
SmiMI CAYNNNNRTG 2 cut(s) 441, 820
SmlI CTYRAG 2 cut(s) 653, 695
SmoI CTYRAG 2 cut(s) 653, 695
Sse9I AATT 7 cut(s) 127, 321, 474, 561, 586, 754, 777
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 5 cut(s) 226, 229, 259, 371, 868
SspMI CTAG 2 cut(s) 555, 635
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 1 cut(s) 853
TaiI ACGT 2 cut(s) 21, 877
TaqI TCGA 2 cut(s) 253, 384
TasI AATT 7 cut(s) 127, 321, 474, 561, 586, 754, 777
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 1 cut(s) 752
Tru9I TTAA 1 cut(s) 752
TscAI CASTG 1 cut(s) 502
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 467, 771
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 603
XapI RAATTY 6 cut(s) 127, 474, 561, 586, 754, 777
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 291, 417
XmnI GAANNNNTTC 2 cut(s) 90, 579
XspI CTAG 2 cut(s) 555, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.