MD15G1221700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013587
Physical Location & Seq
Forward (+)
17964329 .. 17966578
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1221700.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCGACGGGAAACGATGTCGTAGAGATTGATAGCTTGGAGAAAGGGTTACTGTCAGAAACTGCAACCGAGGCTGAAGCTGAAGAAGCAGTTCTGTATTCAGCTTCGTTTCAGGAAATGGAGGAGAATTTTGTCAAGTACCAAACAGCACAATGGCTTCTTTACTCTGCGCTTTTGATACTGGCGTGGGGGATTGGGATTTTTATGCTGCTTTATCTGCCCGTCCGGCGCCACATTCTGCGAAAGGATATTCGTTCGCGGAAGCTCTATCTCACTCCCAATGCCATTGTCTACAAGTATGCAAAGCCAGTTCCATTTCCAATTTTCGGGGTGTTGAAGAAAGAGAAGCATGTTTTGTTGCCTTCAGTGGCGGATGTTGTGATCGAGCAAGGATATCTGCAGTCTCTCTTCGGTGTCTACTCACTTAGAATAGAGCATGTTGGTGTCAGAAGGCCGCCAAGCGATGATGTTCAAATTCATGGCATTGCAAATCCCAGTGCTTTCAGGAAGGCGGTCCTCATGCGCCTTGCAAGCATGACAAATGAGGTCTTCACTAGACAAGTTTCCACACTCGAAGATATTCCAAATTTGAAGCTTCAGGGGTCCCCGTCAAAGTCTCTCAGGTATGGGGATCTAACGCTACTGCAGAAACTTGAGGAAGTAGGAAGTTCTGTAAAGAGGGTTCAAACTATACTCGAGGAACAACACCCTCAAACGCTAGAACCTATAGGTGGAAGCGCGCGAAGAGTGTTAAATTTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.62

Weight (kDa)

7.89

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 82 - 180 6.1e-49 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013587)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09130
malus_domestica MD02G1096900.v1.1 MD15G1221700.v1.1
prunus_persica Prupe.7G194000_v2.0.a1
pyrus_communis pycom02g07690 pycom15g19620
rosa_chinensis RchiOBHm_Chr2g0095521
rosa_laevigata RLG00000016575
rosa_multiflora Rmu_sc0002541.1_g000031
rosa_roxburghii Rroxscaffold_2G00146230
rosa_rugosa Rorug02G0052100 Rorug02G0471700
rosa_samantha Rh2AG099900 Rh2BG101300 Rh2CG103500 Rh2DG100800
rosa_wichuraiana Rw2G007580 Rw7G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 228
AccI GTMKAC 2 cut(s) 291, 417
AccII CGCG 3 cut(s) 259, 740, 742
AciI CCGC 4 cut(s) 259, 371, 455, 512
AclWI GGATC 1 cut(s) 639
AcsI RAATTY 4 cut(s) 127, 474, 586, 754
AcuI CTGAAG 4 cut(s) 96, 102, 348, 581
AcyI GRCGYC 1 cut(s) 229
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 2 cut(s) 326, 731
AgsI TTSAA 4 cut(s) 337, 473, 592, 686
AluBI AGCT 5 cut(s) 36, 80, 104, 265, 595
AluI AGCT 5 cut(s) 36, 80, 104, 265, 595
Alw26I GTCTC 2 cut(s) 408, 621
AlwI GGATC 1 cut(s) 639
AlwNI CAGNNNCTG 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 695
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 4 cut(s) 127, 474, 586, 754
Asp700I GAANNNNTTC 2 cut(s) 90, 579
AspLEI GCGC 5 cut(s) 172, 231, 525, 740, 742
AspS9I GGNCC 2 cut(s) 514, 603
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 2 cut(s) 514, 603
BaeI ACNNNNGTAYC 2 cut(s) 170, 203
BanI GGYRCC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 195
BcoDI GTCTC 2 cut(s) 408, 621
BfaI CTAG 2 cut(s) 555, 719
BfmI CTRYAG 3 cut(s) 398, 644, 726
BfoI RGCGCY 1 cut(s) 232
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 209, 455
BlsI GCNGC 2 cut(s) 210, 456
Bme18I GGWCC 2 cut(s) 514, 603
BmeT110I CYCGRG 1 cut(s) 695
BmgT120I GGNCC 2 cut(s) 514, 603
BmiI GGNNCC 3 cut(s) 230, 604, 605
BmrI ACTGGG 1 cut(s) 489
BmuI ACTGGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 731
Bse1I ACTGG 3 cut(s) 186, 308, 495
Bse3DI GCAATG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 2 cut(s) 326, 731
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 634
BseNI ACTGG 3 cut(s) 186, 308, 495
BsePI GCGCGC 1 cut(s) 738
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 195
Bsh1236I CGCG 3 cut(s) 259, 740, 742
BshFI GGCC 1 cut(s) 454
BshNI GGYRCC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 1 cut(s) 226
BslFI GGGAC 1 cut(s) 589
BslI CCNNNNNNNGG 2 cut(s) 326, 731
BsmAI GTCTC 2 cut(s) 408, 621
BsmFI GGGAC 1 cut(s) 589
BsnI GGCC 1 cut(s) 454
BsoBI CYCGRG 1 cut(s) 695
Bsp143I GATC 2 cut(s) 381, 631
BspACI CCGC 4 cut(s) 259, 371, 455, 512
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 3 cut(s) 259, 740, 742
BspLI GGNNCC 3 cut(s) 230, 604, 605
BspMAI CTGCAG 2 cut(s) 402, 648
BspPI GGATC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 3 cut(s) 186, 308, 495
BssECI CCNNGG 1 cut(s) 69
BssHII GCGCGC 1 cut(s) 738
BssMI GATC 2 cut(s) 381, 631
BssNI GRCGYC 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 54
Bst6I CTCTTC 2 cut(s) 413, 739
BstACI GRCGYC 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 532, 740
BstDEI CTNAG 2 cut(s) 425, 620
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 3 cut(s) 259, 740, 742
BstH2I RGCGCY 1 cut(s) 232
BstHHI GCGC 5 cut(s) 172, 231, 525, 740, 742
BstKTI GATC 2 cut(s) 384, 634
BstMAI GTCTC 2 cut(s) 408, 621
BstMBI GATC 2 cut(s) 381, 631
BstMWI GCNNNNNNNGC 5 cut(s) 71, 86, 217, 226, 531
BstNSI RCATGY 2 cut(s) 353, 440
BstSFI CTRYAG 3 cut(s) 398, 644, 726
BstUI CGCG 3 cut(s) 259, 740, 742
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 1 cut(s) 454
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 372, 502
Cac8I GCNNGC 2 cut(s) 532, 740
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 5 cut(s) 172, 231, 525, 740, 742
Cfr13I GGNCC 2 cut(s) 514, 603
Csp6I GTAC 1 cut(s) 139
CviAII CATG 5 cut(s) 350, 437, 479, 520, 535
CviJI RGCY 9 cut(s) 36, 74, 80, 104, 157, 265, 307, 454, 595
CviKI_1 RGCY 9 cut(s) 36, 74, 80, 104, 157, 265, 307, 454, 595
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 425, 620
DinI GGCGCC 1 cut(s) 230
DpnI GATC 2 cut(s) 383, 633
DpnII GATC 2 cut(s) 381, 631
Eam1104I CTCTTC 2 cut(s) 413, 739
EarI CTCTTC 2 cut(s) 413, 739
EciI GGCGGA 1 cut(s) 386
Eco32I GATATC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 514, 603
Eco57I CTGAAG 4 cut(s) 96, 102, 348, 581
Eco88I CYCGRG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 603
EcoRV GATATC 1 cut(s) 395
EgeI GGCGCC 1 cut(s) 230
EheI GGCGCC 1 cut(s) 230
FaeI CATG 5 cut(s) 353, 440, 482, 523, 538
FaqI GGGAC 1 cut(s) 589
FatI CATG 5 cut(s) 349, 436, 478, 519, 534
FblI GTMKAC 2 cut(s) 291, 417
Fnu4HI GCNGC 2 cut(s) 209, 455
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 209, 455
FspBI CTAG 2 cut(s) 555, 719
GlaI GCGC 5 cut(s) 171, 230, 524, 739, 741
GluI GCNGC 2 cut(s) 209, 455
HaeII RGCGCY 1 cut(s) 232
HaeIII GGCC 1 cut(s) 454
HapII CCGG 1 cut(s) 226
HhaI GCGC 5 cut(s) 172, 231, 525, 740, 742
Hin1I GRCGYC 1 cut(s) 229
Hin1II CATG 5 cut(s) 353, 440, 482, 523, 538
Hin6I GCGC 5 cut(s) 170, 229, 523, 738, 740
HinP1I GCGC 5 cut(s) 170, 229, 523, 738, 740
HindIII AAGCTT 1 cut(s) 593
HpaII CCGG 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 292, 418
Hpy188I TCNGA 2 cut(s) 58, 449
Hpy188III TCNNGA 2 cut(s) 113, 505
Hpy8I GTNNAC 2 cut(s) 292, 418
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 372, 444, 502
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 6 cut(s) 65, 302, 400, 488, 530, 646
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 86, 217, 226, 531
HpyF3I CTNAG 2 cut(s) 425, 620
Hsp92I GRCGYC 1 cut(s) 229
Hsp92II CATG 5 cut(s) 353, 440, 482, 523, 538
HspAI GCGC 5 cut(s) 170, 229, 523, 738, 740
KasI GGCGCC 1 cut(s) 228
KflI GGGWCCC 1 cut(s) 603
Kzo9I GATC 2 cut(s) 381, 631
LpnPI CCDG 8 cut(s) 98, 167, 239, 321, 490, 508, 584, 607
Lsp1109I GCAGC 1 cut(s) 195
MaeI CTAG 2 cut(s) 555, 719
MaeIII GTNAC 1 cut(s) 48
MalI GATC 2 cut(s) 383, 633
MboI GATC 2 cut(s) 381, 631
MboII GAAGA 6 cut(s) 95, 349, 400, 541, 587, 756
MflI RGATCY 1 cut(s) 631
MluCI AATT 5 cut(s) 127, 321, 474, 586, 754
Mly113I GGCGCC 1 cut(s) 229
MnlI CCTC 8 cut(s) 64, 115, 527, 538, 649, 672, 691, 720
MroXI GAANNNNTTC 2 cut(s) 90, 579
MseI TTAA 1 cut(s) 752
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 1 cut(s) 226
MvnI CGCG 3 cut(s) 259, 740, 742
MwoI GCNNNNNNNGC 5 cut(s) 71, 86, 217, 226, 531
NarI GGCGCC 1 cut(s) 229
NdeII GATC 2 cut(s) 381, 631
NlaIII CATG 5 cut(s) 353, 440, 482, 523, 538
NlaIV GGNNCC 3 cut(s) 230, 604, 605
NspI RCATGY 2 cut(s) 353, 440
PaeR7I CTCGAG 1 cut(s) 695
PauI GCGCGC 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 90, 579
PkrI GCNGC 2 cut(s) 210, 456
PluTI GGCGCC 1 cut(s) 232
PpuMI RGGWCCY 1 cut(s) 603
Psp5II RGGWCCY 1 cut(s) 603
PspN4I GGNNCC 3 cut(s) 230, 604, 605
PspPI GGNCC 2 cut(s) 514, 603
PspPPI RGGWCCY 1 cut(s) 603
PspXI VCTCGAGB 1 cut(s) 695
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstI CTGCAG 2 cut(s) 402, 648
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 631
PteI GCGCGC 1 cut(s) 738
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 1 cut(s) 752
SatI GCNGC 2 cut(s) 209, 455
Sau3AI GATC 2 cut(s) 381, 631
Sau96I GGNCC 2 cut(s) 514, 603
SetI ASST 9 cut(s) 38, 82, 106, 267, 549, 597, 626, 727, 733
SfcI CTRYAG 3 cut(s) 398, 644, 726
SfoI GGCGCC 1 cut(s) 230
Sfr274I CTCGAG 1 cut(s) 695
SinI GGWCC 2 cut(s) 514, 603
SlaI CTCGAG 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 2 cut(s) 653, 695
SmoI CTYRAG 2 cut(s) 653, 695
Sse9I AATT 5 cut(s) 127, 321, 474, 586, 754
SsiI CCGC 4 cut(s) 259, 371, 455, 512
SspDI GGCGCC 1 cut(s) 228
SspMI CTAG 2 cut(s) 555, 719
TaaI ACNGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 384, 573, 696
TasI AATT 5 cut(s) 127, 321, 474, 586, 754
TauI GCSGC 1 cut(s) 457
Tru1I TTAA 1 cut(s) 752
Tru9I TTAA 1 cut(s) 752
TscAI CASTG 2 cut(s) 372, 502
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 2 cut(s) 372, 502
VpaK11BI GGWCC 2 cut(s) 514, 603
XapI RAATTY 4 cut(s) 127, 474, 586, 754
XceI RCATGY 2 cut(s) 353, 440
XhoI CTCGAG 1 cut(s) 695
XmiI GTMKAC 2 cut(s) 291, 417
XmnI GAANNNNTTC 2 cut(s) 90, 579
XspI CTAG 2 cut(s) 555, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.