MD02G1102600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017214
Physical Location & Seq
Reverse (-)
8225074 .. 8227220
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1102600.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGCAAGCCTGCAGATTTGTCTCTTGAACCATAAAAAAATCATCATCTGTTTAAGTACTCTACTTTTCTCAATTGGGTTTGGATCATGTGGTAGTGATCAAACACTAAGCAGGAGGACTGCTGCCTTGTTCATCTTTGGTGACTCCACAGTTGATCCTGGCAACAACAATCACATCACCACCATTCCTGAGAACCAAGCACATTACGAGCCATATGGGAAGAATGGCTTCTTTGAAACTCCCACCGGACGCTTCTCCGACGGTCGAATCATCGTAGATTATATAGCTGAATATGCAAAGCTGCCACTAATCCCTCCATTTTTACAACCATCAACTGCTGATTATGCCAATGGTGTTAACTTTGCATCTGGTGGAGCTGGAGTCCTTTCTGAAACCAATCAAGGATTGGCGATTGATCTTCAAACACAATTGAAAAACTTTGAAGAGGTGCAAAAATCATTGACTGAAAAATTAGGAGAAGTGGAAGCAAAGGAAGTGATATCAGAAGCAGTTTATTTCATTAGCATGGGAAGCAACGATTACATGGGTGGTTATTTAGGCAGTCCAAAAATGCAGGAACAATATAACCCTGAACAATTTGTGGGAATGGTCATTGGAAACTTGACACAGACAATTCAAGCGTTATATGAGAAAGGCGGGCGAAAGTTTGCCTTCCTAAGTTTGTGTCCACTAGGTTGCTTGCCTGCCTTTAGAGCTCTGAACAGTATGAACCCGGAATCCGGTGGAGGGGGATGTTTTGAAGCAGCTTCTGCCCTTGCACTAGCACATAACAATGCCCTAAAATCTGTACTCAACAGTCTTCAATATATGCTCAAGGATTTCAAGTACTGCAACTCCAATTTTTATGATTGGCTCCTTGAAAGAATAGAGGATCCCTCCAAATATGGTTTCAAGGATGGAGTGAATGCTTGTTGTGGAACTGGACCATATGGGGGCATATTTAGCTGTGGAGGCACAAAGGAAGTTAAGGATTACCAGTTATGCGATAATGCTGATGAATATATATGGTGGGATTCTTTTCATCCCACTGAGAGGATCCACAAGCAGCTTGCAAAGGCTCTCTGGAATGGACCCCCTTCTGATGTAGGGCCATACAACCTAGAACATCTCTTTTCTGATAAGGAGAAGCTCACCATTGGCGACGTGGTTGATGACCCAGAAGCACAGCAATTTTCACTTCTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

403

Amino Acids

44.2

Weight (kDa)

4.94

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 361 3.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09430
malus_domestica MD02G1102600.v1.1
prunus_persica Prupe.7G191700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095981
rosa_laevigata RLG00000016614
rosa_multiflora Rmu_sc0002497.1_g000009
rosa_roxburghii Rroxscaffold_2G00145890
rosa_rugosa Rorug02G0055600
rosa_samantha Rh2AG103500 Rh2BG105000 Rh2CG107500 Rh2DG105400
rosa_wichuraiana Rw2G007890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 657
AclWI GGATC 6 cut(s) 91, 149, 887, 900, 1051, 1064
AfaI GTAC 3 cut(s) 58, 810, 848
AfiI CCNNNNNNNGG 4 cut(s) 740, 747, 953, 1053
AjiI CACGTC 1 cut(s) 1165
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 8 cut(s) 287, 301, 377, 716, 767, 966, 1069, 1150
AluI AGCT 8 cut(s) 287, 301, 377, 716, 767, 966, 1069, 1150
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 6 cut(s) 91, 149, 887, 900, 1051, 1064
AlwNI CAGNNNCTG 1 cut(s) 770
AoxI GGCC 1 cut(s) 1109
ApeKI GCWGC 4 cut(s) 122, 301, 764, 1066
Asp700I GAANNNNTTC 1 cut(s) 227
AspS9I GGNCC 3 cut(s) 944, 1091, 1109
AsuC2I CCSGG 1 cut(s) 734
AsuHPI GGTGA 3 cut(s) 152, 169, 1144
AvaII GGWCC 2 cut(s) 944, 1091
BamHI GGATCC 2 cut(s) 892, 1056
BanII GRGCYC 1 cut(s) 718
BbsI GAAGAC 1 cut(s) 812
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 4 cut(s) 109, 288, 776, 1078
BccI CCATC 2 cut(s) 337, 911
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 1 cut(s) 734
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 3 cut(s) 692, 782, 1121
BfmI CTRYAG 1 cut(s) 11
BisI GCNGC 4 cut(s) 123, 302, 765, 1067
BlsI GCNGC 4 cut(s) 124, 303, 766, 1068
BmcAI AGTACT 2 cut(s) 58, 848
Bme1390I CCNGG 2 cut(s) 159, 734
Bme18I GGWCC 2 cut(s) 944, 1091
BmgBI CACGTC 1 cut(s) 1165
BmgT120I GGNCC 3 cut(s) 944, 1091, 1109
BmiI GGNNCC 4 cut(s) 875, 894, 1058, 1093
BmrFI CCNGG 2 cut(s) 159, 734
BmsI GCATC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 812
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 1 cut(s) 734
BsaWI WCCGGW 2 cut(s) 245, 740
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 839, 869
Bsc4I CCNNNNNNNGG 4 cut(s) 740, 747, 953, 1053
Bse1I ACTGG 2 cut(s) 946, 997
BseBI CCWGG 1 cut(s) 159
BseGI GGATG 3 cut(s) 758, 922, 1042
BseLI CCNNNNNNNGG 4 cut(s) 740, 747, 953, 1053
BseMII CTCAG 2 cut(s) 180, 1041
BseNI ACTGG 2 cut(s) 946, 997
BseXI GCAGC 4 cut(s) 109, 288, 776, 1078
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 1111
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 718
BsiSI CCGG 3 cut(s) 246, 734, 741
BslI CCNNNNNNNGG 4 cut(s) 740, 747, 953, 1053
BsmAI GTCTC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 931
BsnI GGCC 1 cut(s) 1111
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
BspACI CCGC 1 cut(s) 657
BspANI GGCC 1 cut(s) 1111
BspCNI CTCAG 2 cut(s) 181, 1042
BspLI GGNNCC 4 cut(s) 875, 894, 1058, 1093
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 6 cut(s) 91, 149, 887, 900, 1051, 1064
BsrI ACTGG 2 cut(s) 946, 997
BssMI GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 4 cut(s) 151, 263, 725, 818
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 770
BstC8I GCNNGC 6 cut(s) 7, 11, 659, 701, 705, 1071
BstDEI CTNAG 4 cut(s) 107, 189, 677, 1050
BstF5I GGATG 3 cut(s) 758, 922, 1042
BstKTI GATC 6 cut(s) 86, 100, 157, 418, 895, 1059
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 7 cut(s) 293, 344, 531, 713, 770, 963, 972
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 157, 732
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 4 cut(s) 109, 288, 776, 1078
BstV2I GAAGAC 1 cut(s) 812
BstX2I RGATCY 2 cut(s) 892, 1056
BstYI RGATCY 2 cut(s) 892, 1056
BsuRI GGCC 1 cut(s) 1111
BtrI CACGTC 1 cut(s) 1165
BtsCI GGATG 3 cut(s) 758, 922, 1042
BtsIMutI CAGTG 1 cut(s) 1047
Cac8I GCNNGC 6 cut(s) 7, 11, 659, 701, 705, 1071
CaiI CAGNNNCTG 1 cut(s) 770
Cfr13I GGNCC 3 cut(s) 944, 1091, 1109
CseI GACGC 1 cut(s) 258
Csp6I GTAC 3 cut(s) 57, 809, 847
CviAII CATG 3 cut(s) 87, 526, 544
CviQI GTAC 3 cut(s) 57, 809, 847
DdeI CTNAG 4 cut(s) 107, 189, 677, 1050
DpnI GATC 6 cut(s) 85, 99, 156, 417, 894, 1058
DpnII GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 718
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 944, 1091
Eco53kI GAGCTC 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 716
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 718
FaeI CATG 3 cut(s) 90, 529, 547
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 656, 688
FatI CATG 3 cut(s) 86, 525, 543
FauI CCCGC 1 cut(s) 650
FauNDI CATATG 2 cut(s) 214, 949
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 4 cut(s) 123, 302, 765, 1067
FokI GGATG 3 cut(s) 765, 929, 1029
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 4 cut(s) 123, 302, 765, 1067
FspBI CTAG 3 cut(s) 692, 782, 1121
GluI GCNGC 4 cut(s) 123, 302, 765, 1067
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 1 cut(s) 1111
HapII CCGG 3 cut(s) 246, 734, 741
HgaI GACGC 1 cut(s) 258
Hin1II CATG 3 cut(s) 90, 529, 547
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 5 cut(s) 143, 267, 381, 737, 1034
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 3 cut(s) 246, 734, 741
HphI GGTGA 3 cut(s) 152, 169, 1144
Hpy166II GTNNAC 2 cut(s) 358, 689
Hpy188I TCNGA 7 cut(s) 259, 391, 505, 720, 1102, 1138, 1203
Hpy188III TCNNGA 3 cut(s) 25, 188, 1084
Hpy8I GTNNAC 2 cut(s) 358, 689
Hpy99I CGWCG 2 cut(s) 263, 1166
HpyAV CCTTC 2 cut(s) 682, 1107
HpyCH4III ACNGT 4 cut(s) 151, 263, 725, 818
HpyCH4IV ACGT 1 cut(s) 1164
HpyCH4V TGCA 8 cut(s) 13, 296, 365, 451, 574, 779, 852, 1073
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 344, 531, 713, 770, 963, 972
HpyF3I CTNAG 4 cut(s) 107, 189, 677, 1050
HpySE526I ACGT 1 cut(s) 1164
Hsp92II CATG 3 cut(s) 90, 529, 547
Ksp22I TGATCA 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
LmnI GCTCC 2 cut(s) 374, 879
Lsp1109I GCAGC 4 cut(s) 109, 288, 776, 1078
LweI GCATC 1 cut(s) 374
MaeI CTAG 3 cut(s) 692, 782, 1121
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 1 cut(s) 140
MalI GATC 6 cut(s) 85, 99, 156, 417, 894, 1058
MboI GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
MboII GAAGA 4 cut(s) 232, 410, 455, 812
MfeI CAATTG 2 cut(s) 72, 428
MflI RGATCY 2 cut(s) 892, 1056
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 7 cut(s) 72, 428, 470, 596, 633, 859, 1190
MlyI GAGTC 2 cut(s) 137, 390
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 8 cut(s) 108, 324, 439, 740, 883, 907, 965, 1047
MroXI GAANNNNTTC 1 cut(s) 227
MseI TTAA 3 cut(s) 53, 357, 987
MslI CAYNNNNRTG 2 cut(s) 524, 792
MspI CCGG 3 cut(s) 246, 734, 741
MspR9I CCNGG 2 cut(s) 159, 734
MunI CAATTG 2 cut(s) 72, 428
Mva1269I GAATGC 1 cut(s) 931
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 7 cut(s) 293, 344, 531, 713, 770, 963, 972
NciI CCSGG 1 cut(s) 734
NdeI CATATG 2 cut(s) 214, 949
NdeII GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
NlaIII CATG 3 cut(s) 90, 529, 547
NlaIV GGNNCC 4 cut(s) 875, 894, 1058, 1093
NmuCI GTSAC 1 cut(s) 140
PctI GAATGC 1 cut(s) 931
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 267, 737, 1034
PkrI GCNGC 4 cut(s) 124, 303, 766, 1068
PleI GAGTC 2 cut(s) 137, 389
PpsI GAGTC 2 cut(s) 137, 389
Psp124BI GAGCTC 1 cut(s) 718
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 4 cut(s) 875, 894, 1058, 1093
PspPI GGNCC 3 cut(s) 944, 1091, 1109
PstI CTGCAG 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 2 cut(s) 892, 1056
RsaI GTAC 3 cut(s) 58, 810, 848
RsaNI GTAC 3 cut(s) 57, 809, 847
RseI CAYNNNNRTG 2 cut(s) 524, 792
SacI GAGCTC 1 cut(s) 718
SaqAI TTAA 3 cut(s) 53, 357, 987
SatI GCNGC 4 cut(s) 123, 302, 765, 1067
Sau3AI GATC 6 cut(s) 83, 97, 154, 415, 892, 1056
Sau96I GGNCC 3 cut(s) 944, 1091, 1109
ScaI AGTACT 2 cut(s) 58, 848
SchI GAGTC 2 cut(s) 137, 390
ScrFI CCNGG 2 cut(s) 159, 734
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 11
SinI GGWCC 2 cut(s) 944, 1091
SmiMI CAYNNNNRTG 2 cut(s) 524, 792
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
Sse9I AATT 7 cut(s) 72, 428, 470, 596, 633, 859, 1190
SsiI CCGC 1 cut(s) 657
SspMI CTAG 3 cut(s) 692, 782, 1121
SstI GAGCTC 1 cut(s) 718
StyD4I CCNGG 2 cut(s) 157, 732
TaaI ACNGT 4 cut(s) 151, 263, 725, 818
TaiI ACGT 1 cut(s) 1167
TaqI TCGA 1 cut(s) 265
TasI AATT 7 cut(s) 72, 428, 470, 596, 633, 859, 1190
TatI WGTACW 3 cut(s) 56, 808, 846
TfiI GAWTC 3 cut(s) 267, 737, 1034
Tru1I TTAA 3 cut(s) 53, 357, 987
Tru9I TTAA 3 cut(s) 53, 357, 987
TscAI CASTG 1 cut(s) 1054
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 4 cut(s) 122, 301, 764, 1066
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 5 cut(s) 121, 508, 743, 1031, 1032
TspRI CASTG 1 cut(s) 1054
VpaK11BI GGWCC 2 cut(s) 944, 1091
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 1162
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 3 cut(s) 692, 782, 1121
ZrmI AGTACT 2 cut(s) 58, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.