Rh2CG107500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017214
Physical Location & Seq
Reverse (-)
9349317 .. 9355069
5753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG107500.1

Sequence Viewer

Length: 879 bp
ATGGCTAGCCTGCAGATTTCTCTCTCGGGTCAGCTAATCATAATCTGTGTGATGATGATGAGTAGCTGCCATGGAGCATTTGCTACTAGTGTTGCCTTGTTCATTTTCGGGGACTCGACTGTGGATACTGGCAACAACAACTACATCACCACCGTCCCGGAAAACCAAGCACATTACAAGCCCTACGGCCAGAATGGTTTCTTTGACCACCCCACCGGACGCTTCTCCGATGGTCGTGTCATTGTAGACTACATAGCGGAGTATGCAAAGCTGCCAATAATCCCTCCATTTTTGGGACCATCTGCTGACTACACAAATGGAGTCAACTTTGCATCGGGTGGAGCTGGTGTTCTTCCGGAAACCAATCAAGGATCGGCGATTGATCTTCCGACACAATTGAGATACTTTGAAGAGGTGCAAAAACGGTTCACAAAAGAATTGGGAGAAGAGAAAGCAAAGGAAGTGATATCAGAGGCAGTGTATTTCATTAGTATTGGAAGCAATGACTATATGGGCGGTTACCTAGGTAATCCAAAAATGCAGGAACAATATAACCCTGAACAATATGTGGGGATGGTCATCGGAAACTTGACACAGGCAATCCAAGTGCTGTATGAAAAAGGAGGTAGAAAATTTGGTTTTCTAAGATTGACTCCGCTTGGTTGCTTGCCTGCCTTTAGAGCTGTGAACCTCAGAGCCATGAAAGGAGGAAGGGGCTGTTTCGAAGGAGCTTCTGCCCTTGCACTAGCTCATAATGATGCGCTTAATACTGTGCTCGCAAACCTTGGATATGTTCTCAAAGAGTTCAAGCACTGCAGCTCCAATTTCTATGACTGGCTCCAGGAAAGAATAGATCATCCTTATAAATATGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

292

Amino Acids

32.13

Weight (kDa)

5.88

Isoelectric Point (pI)

28.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 275 3.3e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09430
malus_domestica MD02G1102600.v1.1
prunus_persica Prupe.7G191700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095981
rosa_laevigata RLG00000016614
rosa_multiflora Rmu_sc0002497.1_g000009
rosa_roxburghii Rroxscaffold_2G00145890
rosa_rugosa Rorug02G0055600
rosa_samantha Rh2AG103500 Rh2BG105000 Rh2CG107500 Rh2DG105400
rosa_wichuraiana Rw2G007890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AccI GTMKAC 1 cut(s) 246
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 3 cut(s) 257, 516, 656
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 632
AfaI GTAC 1 cut(s) 874
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 410, 808
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 840
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 8 cut(s) 34, 66, 271, 344, 683, 731, 749, 819
AluI AGCT 8 cut(s) 34, 66, 271, 344, 683, 731, 749, 819
Alw21I GWGCWC 1 cut(s) 777
AlwI GGATC 1 cut(s) 379
Ama87I CYCGRG 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 3 cut(s) 66, 271, 816
ApoI RAATTY 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 197
AspA2I CCTAGG 1 cut(s) 523
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 139
AsuII TTCGAA 1 cut(s) 723
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 1 cut(s) 296
AvrII CCTAGG 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 777
BbvI GCAGC 3 cut(s) 53, 258, 828
BccI CCATC 3 cut(s) 224, 307, 568
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 842
BciVI GTATCC 1 cut(s) 118
BcnI CCSGG 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 6, 87, 524, 746
BfmI CTRYAG 2 cut(s) 11, 814
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 3 cut(s) 67, 272, 817
BlnI CCTAGG 1 cut(s) 523
BlsI GCNGC 3 cut(s) 68, 273, 818
Bme1390I CCNGG 2 cut(s) 158, 842
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 297, 839
BmrFI CCNGG 2 cut(s) 158, 842
BmsI GCATC 2 cut(s) 341, 748
BmtI GCTAGC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 824
Bpu14I TTCGAA 1 cut(s) 723
BpuMI CCSGG 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 578
BsaJI CCNNGG 3 cut(s) 70, 523, 784
BsaWI WCCGGW 2 cut(s) 215, 355
BsaXI ACNNNNNCTCC 4 cut(s) 333, 363, 803, 833
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 133, 839
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 578
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 3 cut(s) 70, 523, 784
BseGI GGATG 2 cut(s) 579, 856
BseJI GATNNNNATC 1 cut(s) 578
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 2 cut(s) 133, 839
BseXI GCAGC 3 cut(s) 53, 258, 828
BshFI GGCC 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 3 cut(s) 158, 216, 356
BslFI GGGAC 3 cut(s) 125, 140, 309
BslI CCNNNNNNNGG 1 cut(s) 293
BsmFI GGGAC 3 cut(s) 125, 140, 309
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 723
Bsp1286I GDGCHC 1 cut(s) 777
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 3 cut(s) 371, 382, 853
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 3 cut(s) 257, 516, 656
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 1 cut(s) 705
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 297, 839
BspMAI CTGCAG 2 cut(s) 15, 818
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 2 cut(s) 133, 839
BssECI CCNNGG 3 cut(s) 70, 523, 784
BssMI GATC 3 cut(s) 371, 382, 853
BssT1I CCWWGG 3 cut(s) 70, 523, 784
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 4 cut(s) 121, 154, 426, 772
Bst6I CTCTTC 2 cut(s) 405, 441
BstBI TTCGAA 1 cut(s) 723
BstC8I GCNNGC 5 cut(s) 7, 11, 668, 672, 777
BstDEI CTNAG 2 cut(s) 644, 692
BstDSI CCRYGG 1 cut(s) 70
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 2 cut(s) 579, 856
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 3 cut(s) 374, 385, 856
BstMBI GATC 3 cut(s) 371, 382, 853
BstMWI GCNNNNNNNGC 2 cut(s) 263, 680
BstNI CCWGG 1 cut(s) 842
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 156, 840
BstSFI CTRYAG 2 cut(s) 11, 814
BstV1I GCAGC 3 cut(s) 53, 258, 828
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 579, 856
BtsI GCAGTG 2 cut(s) 483, 811
BtsIMutI CAGTG 2 cut(s) 483, 811
Cac8I GCNNGC 5 cut(s) 7, 11, 668, 672, 777
CfoI GCGC 1 cut(s) 763
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 873
CviAII CATG 2 cut(s) 71, 700
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 2 cut(s) 644, 692
DpnI GATC 3 cut(s) 373, 384, 855
DpnII GATC 3 cut(s) 371, 382, 853
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 405, 441
EarI CTCTTC 2 cut(s) 405, 441
Eco130I CCWWGG 3 cut(s) 70, 523, 784
Eco32I GATATC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 840
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 3 cut(s) 70, 523, 784
ErhI CCWWGG 3 cut(s) 70, 523, 784
FaeI CATG 2 cut(s) 74, 703
FaqI GGGAC 3 cut(s) 125, 140, 309
FatI CATG 2 cut(s) 70, 699
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 67, 272, 817
FokI GGATG 2 cut(s) 586, 843
Fsp4HI GCNGC 3 cut(s) 67, 272, 817
FspBI CTAG 4 cut(s) 6, 87, 524, 746
GlaI GCGC 1 cut(s) 762
GluI GCNGC 3 cut(s) 67, 272, 817
GsuI CTGGAG 1 cut(s) 824
HaeIII GGCC 1 cut(s) 189
HapII CCGG 3 cut(s) 158, 216, 356
HgaI GACGC 1 cut(s) 228
HhaI GCGC 1 cut(s) 763
Hin1II CATG 2 cut(s) 74, 703
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 3 cut(s) 113, 321, 652
HpaII CCGG 3 cut(s) 158, 216, 356
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 4 cut(s) 247, 325, 429, 688
Hpy188I TCNGA 5 cut(s) 229, 390, 472, 584, 695
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 4 cut(s) 247, 325, 429, 688
HpyAV CCTTC 2 cut(s) 705, 719
HpyCH4III ACNGT 4 cut(s) 121, 154, 426, 772
HpyCH4V TGCA 7 cut(s) 13, 266, 332, 418, 541, 743, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 680
HpyF3I CTNAG 2 cut(s) 644, 692
Hsp92II CATG 2 cut(s) 74, 703
HspAI GCGC 1 cut(s) 761
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 371, 382, 853
LmnI GCTCC 5 cut(s) 74, 341, 728, 824, 843
Lsp1109I GCAGC 3 cut(s) 53, 258, 828
LweI GCATC 2 cut(s) 341, 748
MaeI CTAG 4 cut(s) 6, 87, 524, 746
MaeIII GTNAC 1 cut(s) 518
MalI GATC 3 cut(s) 373, 384, 855
MboI GATC 3 cut(s) 371, 382, 853
MboII GAAGA 4 cut(s) 344, 377, 422, 458
MfeI CAATTG 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 777
MluCI AATT 4 cut(s) 395, 437, 632, 823
MlyI GAGTC 3 cut(s) 107, 330, 646
MmeI TCCRAC 1 cut(s) 413
MnlI CCTC 6 cut(s) 294, 406, 466, 617, 701, 701
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 756
MspI CCGG 3 cut(s) 158, 216, 356
MspR9I CCNGG 2 cut(s) 158, 842
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 842
MwoI GCNNNNNNNGC 2 cut(s) 263, 680
NciI CCSGG 1 cut(s) 158
NcoI CCATGG 1 cut(s) 70
NdeII GATC 3 cut(s) 371, 382, 853
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 74, 703
NlaIV GGNNCC 2 cut(s) 297, 839
NspV TTCGAA 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 197
PfoI TCCNGGA 2 cut(s) 156, 840
PkrI GCNGC 3 cut(s) 68, 273, 818
PleI GAGTC 3 cut(s) 107, 329, 646
PpsI GAGTC 3 cut(s) 107, 329, 646
PsiI TTATAA 1 cut(s) 864
Psp6I CCWGG 1 cut(s) 840
PspEI GGTNACC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 2 cut(s) 297, 839
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 2 cut(s) 15, 818
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
RseI CAYNNNNRTG 1 cut(s) 756
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 3 cut(s) 67, 272, 817
Sau3AI GATC 3 cut(s) 371, 382, 853
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 3 cut(s) 107, 330, 646
ScrFI CCNGG 2 cut(s) 158, 842
SduI GDGCHC 1 cut(s) 777
SfaNI GCATC 2 cut(s) 341, 748
SfcI CTRYAG 2 cut(s) 11, 814
SfuI TTCGAA 1 cut(s) 723
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 756
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 4 cut(s) 395, 437, 632, 823
SsiI CCGC 3 cut(s) 257, 516, 656
SspMI CTAG 4 cut(s) 6, 87, 524, 746
StyD4I CCNGG 2 cut(s) 156, 840
StyI CCWWGG 3 cut(s) 70, 523, 784
TaaI ACNGT 4 cut(s) 121, 154, 426, 772
TaqI TCGA 2 cut(s) 116, 723
TasI AATT 4 cut(s) 395, 437, 632, 823
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 2 cut(s) 483, 818
TseI GCWGC 3 cut(s) 66, 271, 816
TspDTI ATGAA 4 cut(s) 91, 475, 630, 716
TspRI CASTG 2 cut(s) 483, 818
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 632
XmaJI CCTAGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 4 cut(s) 6, 87, 524, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.