RLG00000016614

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017214
Physical Location & Seq
Reverse (-)
8244704 .. 8246704
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016614

Sequence Viewer

Length: 1101 bp
ATGATCTGTGTGATGATGATAAGTAGCTGCCATGGAGCATTTGCTACTAGTAGTGTTGCCTTGTTCATTTTCGGGGACTCGACTGTGGATACTGGCAACAACAACTACATCACCACCGTCCCGGAAAACCAAGCACATTACAAGCCCTACGGCCAGAATGGTTTCTTTGACCACCCCACCGGACGCTTCTCCGATGGTCGTGTCATTGTAGACTACATGGTGGAGTCTTATTTGATGATCGATCGAAATCTTTTTACTAAGATAATATACGTACTGACTCTGTTTGATTGGCAATCTGAATCATTGGCGATTGATCTTCCGACACAATTGAGATACTTTGAAGAGGTGCAAAAACGGTTGACAAAAAAATTGGGAGAAGAGAAAGCAAAGGAAGTGATATCAGAGGCAGTGTATTTCATTAGTATTGGAAGCAATGACTATATGGGCGGTTACTTAGGTAATCCAAAAATGCAGGAACAATATAACCCTGAACAATATGTGGGGATGGTCATCGGAAACTTGACGCAGGCAATCCAGGTGCTGTATGAGAAAGGAGGTAGAAAATTTGGTTTTCTAAGATTGGCTCCGCTTGGTTGCTTGCCTGCCTTTAGAGCTGTGAACCTCAGAGCCACGAAAGGAGGAAGGGGCTGTTTCGAAGGAGCTTCTGCCCTTGCACTAGCTCATAATGATGCGCTTAATACTGTGCTCGCTAACCTTGGATATGTTCTCAAAGAGTTCAAGTACTGCAGCTCCAATTTCTATGACTGGCTCCAGGAAAGAATAGATCATCCTTACAGATATGGTTTCAAGGATGGAATCAATGCTTGTTGCGGAAGCGGACCATATGGAGGTTCGTTCACTTGTGGAGGCACAAAGGAGGATAAAGATTACCAGTTATGTAGCAATGCTGATGATTATATATGGTGGGACTCTTTCCATCCAACAGAGAGGATCCATGAGCAACTTGCAAAGGCTCTCTGGAATGGACCGCCTTCTTCTGTCGGGCCATACAACCTAGAAGGTCTCTTCTTTGAGAAGGAGAAGCTCACCATTGGCAGCATAGTTGATAAACCAGAAGCAGATCAGTTTCAGTCCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

41.15

Weight (kDa)

5.36

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 326 2.8e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09430
malus_domestica MD02G1102600.v1.1
prunus_persica Prupe.7G191700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095981
rosa_laevigata RLG00000016614
rosa_multiflora Rmu_sc0002497.1_g000009
rosa_roxburghii Rroxscaffold_2G00145890
rosa_rugosa Rorug02G0055600
rosa_samantha Rh2AG103500 Rh2BG105000 Rh2CG107500 Rh2DG105400
rosa_wichuraiana Rw2G007890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 210
AciI CCGC 5 cut(s) 447, 587, 831, 837, 989
AclWI GGATC 2 cut(s) 946, 959
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 563
AfaI GTAC 2 cut(s) 273, 743
AfiI CCNNNNNNNGG 1 cut(s) 848
AgsI TTSAA 3 cut(s) 341, 739, 808
AhlI ACTAGT 1 cut(s) 47
AjnI CCWGG 2 cut(s) 534, 771
AluBI AGCT 6 cut(s) 27, 614, 662, 680, 750, 1045
AluI AGCT 6 cut(s) 27, 614, 662, 680, 750, 1045
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 1 cut(s) 1028
AlwI GGATC 2 cut(s) 946, 959
AlwNI CAGNNNCTG 1 cut(s) 541
AoxI GGCC 2 cut(s) 151, 1004
ApeKI GCWGC 3 cut(s) 27, 747, 1056
ApoI RAATTY 1 cut(s) 563
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 3 cut(s) 839, 986, 1004
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 103, 1039
AsuII TTCGAA 1 cut(s) 654
AvaII GGWCC 2 cut(s) 839, 986
BamHI GGATCC 1 cut(s) 951
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 3 cut(s) 14, 759, 1068
BccI CCATC 4 cut(s) 188, 499, 806, 945
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 2 cut(s) 536, 773
BciVI GTATCC 1 cut(s) 82
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 1028
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 3 cut(s) 48, 677, 1016
BfmI CTRYAG 1 cut(s) 745
BfuI GTATCC 1 cut(s) 82
BisI GCNGC 3 cut(s) 28, 748, 1057
BlsI GCNGC 3 cut(s) 29, 749, 1058
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 122, 536, 773
Bme18I GGWCC 2 cut(s) 839, 986
BmgT120I GGNCC 3 cut(s) 839, 986, 1004
BmiI GGNNCC 3 cut(s) 585, 770, 953
BmrFI CCNGG 3 cut(s) 122, 536, 773
BmsI GCATC 1 cut(s) 679
BpmI CTGGAG 1 cut(s) 755
Bpu14I TTCGAA 1 cut(s) 654
BpuMI CCSGG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 271
BsaBI GATNNNNATC 2 cut(s) 246, 509
BsaI GGTCTC 1 cut(s) 1028
BsaJI CCNNGG 2 cut(s) 31, 715
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 3 cut(s) 97, 770, 892
Bse3DI GCAATG 2 cut(s) 439, 910
Bse8I GATNNNNATC 2 cut(s) 246, 509
BseBI CCWGG 2 cut(s) 536, 773
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 31, 715
BseGI GGATG 4 cut(s) 510, 787, 817, 937
BseJI GATNNNNATC 2 cut(s) 246, 509
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 2 cut(s) 439, 910
BseMII CTCAG 1 cut(s) 637
BseNI ACTGG 3 cut(s) 97, 770, 892
BseXI GCAGC 3 cut(s) 14, 759, 1068
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 153, 1006
BshVI ATCGAT 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 2 cut(s) 122, 180
BslFI GGGAC 3 cut(s) 89, 104, 941
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 1 cut(s) 1028
BsmFI GGGAC 3 cut(s) 89, 104, 941
BsnI GGCC 2 cut(s) 153, 1006
Bso31I GGTCTC 1 cut(s) 1028
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 5 cut(s) 447, 587, 831, 837, 989
BspANI GGCC 2 cut(s) 153, 1006
BspCNI CTCAG 1 cut(s) 636
BspDI ATCGAT 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 585, 770, 953
BspMAI CTGCAG 1 cut(s) 749
BspPI GGATC 2 cut(s) 946, 959
BspT104I TTCGAA 1 cut(s) 654
BspTNI GGTCTC 1 cut(s) 1028
BsrDI GCAATG 2 cut(s) 439, 910
BsrI ACTGG 3 cut(s) 97, 770, 892
BssECI CCNNGG 2 cut(s) 31, 715
BssMI GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
BssT1I CCWWGG 2 cut(s) 31, 715
Bst2UI CCWGG 2 cut(s) 536, 773
Bst4CI ACNGT 4 cut(s) 85, 118, 357, 703
Bst6I CTCTTC 3 cut(s) 336, 372, 1031
BstBAI YACGTR 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 4 cut(s) 528, 599, 603, 708
BstDEI CTNAG 4 cut(s) 258, 454, 575, 623
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 4 cut(s) 510, 787, 817, 937
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 7 cut(s) 6, 240, 244, 316, 787, 954, 1084
BstMAI GTCTC 1 cut(s) 1028
BstMBI GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 611
BstNI CCWGG 2 cut(s) 536, 773
BstSCI CCNGG 3 cut(s) 120, 534, 771
BstSFI CTRYAG 1 cut(s) 745
BstSNI TACGTA 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 14, 759, 1068
BstX2I RGATCY 1 cut(s) 951
BstYI RGATCY 1 cut(s) 951
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 153, 1006
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 4 cut(s) 510, 787, 817, 937
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 4 cut(s) 528, 599, 603, 708
CaiI CAGNNNCTG 1 cut(s) 541
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 3 cut(s) 839, 986, 1004
ClaI ATCGAT 1 cut(s) 240
CseI GACGC 2 cut(s) 192, 532
Csp6I GTAC 2 cut(s) 272, 742
CviAII CATG 3 cut(s) 32, 217, 956
CviQI GTAC 2 cut(s) 272, 742
DdeI CTNAG 4 cut(s) 258, 454, 575, 623
DpnI GATC 7 cut(s) 5, 239, 243, 315, 786, 953, 1083
DpnII GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 3 cut(s) 336, 372, 1031
EarI CTCTTC 3 cut(s) 336, 372, 1031
Eco105I TACGTA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 31, 715
Eco31I GGTCTC 1 cut(s) 1028
Eco32I GATATC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 839, 986
EcoRII CCWGG 2 cut(s) 534, 771
EcoRV GATATC 1 cut(s) 399
EcoT14I CCWWGG 2 cut(s) 31, 715
ErhI CCWWGG 2 cut(s) 31, 715
FaeI CATG 3 cut(s) 35, 220, 959
FaqI GGGAC 3 cut(s) 89, 104, 941
FatI CATG 3 cut(s) 31, 216, 955
FauNDI CATATG 1 cut(s) 844
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 28, 748, 1057
FokI GGATG 4 cut(s) 517, 774, 824, 924
Fsp4HI GCNGC 3 cut(s) 28, 748, 1057
FspBI CTAG 3 cut(s) 48, 677, 1016
GlaI GCGC 1 cut(s) 693
GluI GCNGC 3 cut(s) 28, 748, 1057
GsuI CTGGAG 1 cut(s) 755
HaeIII GGCC 2 cut(s) 153, 1006
HapII CCGG 2 cut(s) 122, 180
HgaI GACGC 2 cut(s) 192, 532
HhaI GCGC 1 cut(s) 694
Hin1II CATG 3 cut(s) 35, 220, 959
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 6 cut(s) 77, 224, 277, 299, 816, 929
HpaII CCGG 2 cut(s) 122, 180
HphI GGTGA 2 cut(s) 103, 1039
Hpy166II GTNNAC 4 cut(s) 211, 360, 619, 858
Hpy188I TCNGA 6 cut(s) 193, 298, 321, 403, 515, 626
Hpy188III TCNNGA 1 cut(s) 979
Hpy8I GTNNAC 4 cut(s) 211, 360, 619, 858
HpyAV CCTTC 5 cut(s) 636, 650, 1002, 1013, 1030
HpyCH4III ACNGT 4 cut(s) 85, 118, 357, 703
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 5 cut(s) 349, 472, 674, 747, 968
HpyF10VI GCNNNNNNNGC 1 cut(s) 611
HpyF3I CTNAG 4 cut(s) 258, 454, 575, 623
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 3 cut(s) 35, 220, 959
HspAI GCGC 1 cut(s) 692
Kzo9I GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
LmnI GCTCC 5 cut(s) 35, 589, 659, 755, 774
Lsp1109I GCAGC 3 cut(s) 14, 759, 1068
LweI GCATC 1 cut(s) 679
MaeI CTAG 3 cut(s) 48, 677, 1016
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 449
MalI GATC 7 cut(s) 5, 239, 243, 315, 786, 953, 1083
MboI GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
MboII GAAGA 5 cut(s) 308, 353, 389, 987, 1018
MfeI CAATTG 1 cut(s) 326
MflI RGATCY 1 cut(s) 951
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 4 cut(s) 326, 368, 563, 754
MlyI GAGTC 4 cut(s) 71, 233, 271, 923
MmeI TCCRAC 2 cut(s) 344, 965
MnlI CCTC 9 cut(s) 337, 397, 548, 632, 632, 842, 860, 871, 942
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 1 cut(s) 696
MslI CAYNNNNRTG 1 cut(s) 687
MspI CCGG 2 cut(s) 122, 180
MspR9I CCNGG 3 cut(s) 122, 536, 773
MunI CAATTG 1 cut(s) 326
MvaI CCWGG 2 cut(s) 536, 773
MwoI GCNNNNNNNGC 1 cut(s) 611
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 844
NdeII GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
NlaIII CATG 3 cut(s) 35, 220, 959
NlaIV GGNNCC 3 cut(s) 585, 770, 953
NspV TTCGAA 1 cut(s) 654
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 299, 816
PfoI TCCNGGA 2 cut(s) 120, 771
PkrI GCNGC 3 cut(s) 29, 749, 1058
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 4 cut(s) 71, 232, 271, 923
PpsI GAGTC 4 cut(s) 71, 232, 271, 923
Ppu21I YACGTR 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 534, 771
PspGI CCWGG 2 cut(s) 534, 771
PspN4I GGNNCC 3 cut(s) 585, 770, 953
PspPI GGNCC 3 cut(s) 839, 986, 1004
PstI CTGCAG 1 cut(s) 749
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 951
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 2 cut(s) 273, 743
RsaNI GTAC 2 cut(s) 272, 742
RseI CAYNNNNRTG 1 cut(s) 687
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 3 cut(s) 28, 748, 1057
Sau3AI GATC 7 cut(s) 3, 237, 241, 313, 784, 951, 1081
Sau96I GGNCC 3 cut(s) 839, 986, 1004
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 4 cut(s) 71, 233, 271, 923
ScrFI CCNGG 3 cut(s) 122, 536, 773
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 1 cut(s) 679
SfcI CTRYAG 1 cut(s) 745
SfuI TTCGAA 1 cut(s) 654
SinI GGWCC 2 cut(s) 839, 986
SmiMI CAYNNNNRTG 1 cut(s) 687
SnaBI TACGTA 1 cut(s) 271
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 4 cut(s) 326, 368, 563, 754
SsiI CCGC 5 cut(s) 447, 587, 831, 837, 989
SspMI CTAG 3 cut(s) 48, 677, 1016
StyD4I CCNGG 3 cut(s) 120, 534, 771
StyI CCWWGG 2 cut(s) 31, 715
TaaI ACNGT 4 cut(s) 85, 118, 357, 703
TaiI ACGT 1 cut(s) 273
TaqI TCGA 4 cut(s) 80, 240, 244, 654
TasI AATT 4 cut(s) 326, 368, 563, 754
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 2 cut(s) 299, 816
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TscAI CASTG 1 cut(s) 414
TseI GCWGC 3 cut(s) 27, 747, 1056
TspDTI ATGAA 2 cut(s) 55, 406
TspRI CASTG 1 cut(s) 414
VpaK11BI GGWCC 2 cut(s) 839, 986
XapI RAATTY 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 3 cut(s) 48, 677, 1016
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.