Rroxscaffold_2G00145890

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017214
Physical Location & Seq
Forward (+)
83739499 .. 83743965
4467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145890.1

Sequence Viewer

Length: 1173 bp
ATGGCTAGCCTGCAGATTTCTCTCTCGGGTCAGCTAATCACGATCTGTGTAATGATGATGAGTAGCTGCCATGGAGCATTTGCTACTAGTAGCGTTGCCTTGTTCATTTTCGGGGACTCGACTGTGGATACTGGCAACAACAACTACATCACCACCATCCCGGAAAACCAAGCACATTACAAGCCCTACGGCCAGAATGGTTTCTTTGACCACCCCACCGGACGCTTCTCCGATGGTCGTGTCATTGTAGACTACATAGCGGAGTATGCAAAGCTGCCAATAATCCCTCCATTTTTGGGACCATCTGCTGACTACACAAATGGAGTCAACTTTGCATCGGGTGGAGCTGGTGTTCTTCCGGAAACCAATCAAGGATCGGCGATTGATCTTCCGACACAATTGAGATACTTTGAAGAGGTGCAAAAACGGTTGACAAAAAAATTGGGAGAAGAGAAAGCAAAGGAAGTGATATCAGAGGCAGTGTATTTCATTAGTATTGGAAGCAATGACTATATGGGAGGTTACTTGGGTAATCCAAAAATGCAGGAACAATATAACCCTGAACAATATGTGGGGATGGTCATCGGAAACTTGACGCAGGCAATCCAGGTGCTGTATGAGAAAGGAGGTAGAAAATTTGGTTTTCTAAGATTGATTCCGCTTGGTTGCGTGCCTGCCTTTAGAGCTGTGAACCTCAGAGCCACGAAAGGAGGAAGGGGCTGTTTCGAAGGAGCTTCTGCCCTTGCACTAGCTCATAATGATGCGCTTAATACTGTGCTCGCAAACCTTGGCTATGTTCTCAAAGAGTTCAAGTACTGCACCTCCAATTTCTATGACTGGCTCCAGGAGAGAATAGATCATCCTTACAAATATGGTTTCAAGGACGGAATCAATGCTTGTTGCGGAAGCGGACCATATGGAGGTTCGTTCACCTGTGGAGGCACAAAGGAGGATAAAGATTACCAGTTATGCAGCAATGCTGATGATTATATATGGTGGGACTCTTTCCATCCAACAGAGAGGATCCATGAGCAACTTGCAAAGGCTCTCTGGAATGGACCGCCTTCTTCTGTCGGGCCATACAACCTAGAAGGTCTCTTCTTTGAGAAGGAGAAGCTCACTATTGGCAGCATAGTTGATAAACCAGAAGCAGATCAGTTTCAGTCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.94

Weight (kDa)

5.35

Isoelectric Point (pI)

27.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 350 4.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09430
malus_domestica MD02G1102600.v1.1
prunus_persica Prupe.7G191700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095981
rosa_laevigata RLG00000016614
rosa_multiflora Rmu_sc0002497.1_g000009
rosa_roxburghii Rroxscaffold_2G00145890
rosa_rugosa Rorug02G0055600
rosa_samantha Rh2AG103500 Rh2BG105000 Rh2CG107500 Rh2DG105400
rosa_wichuraiana Rw2G007890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 5 cut(s) 260, 659, 903, 909, 1061
AclWI GGATC 3 cut(s) 382, 1018, 1031
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 635
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 2 cut(s) 296, 920
AgsI TTSAA 3 cut(s) 413, 811, 880
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 2 cut(s) 606, 843
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 8 cut(s) 34, 66, 274, 347, 686, 734, 752, 1117
AluI AGCT 8 cut(s) 34, 66, 274, 347, 686, 734, 752, 1117
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 1100
AlwI GGATC 3 cut(s) 382, 1018, 1031
AlwNI CAGNNNCTG 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 2 cut(s) 190, 1076
ApeKI GCWGC 4 cut(s) 66, 274, 972, 1128
ApoI RAATTY 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 766
AspS9I GGNCC 4 cut(s) 299, 911, 1058, 1076
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 142, 922
AsuII TTCGAA 1 cut(s) 726
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 3 cut(s) 299, 911, 1058
BamHI GGATCC 1 cut(s) 1023
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 4 cut(s) 53, 261, 984, 1140
BccI CCATC 5 cut(s) 164, 227, 310, 571, 1017
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 2 cut(s) 608, 845
BciVI GTATCC 1 cut(s) 121
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 1100
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 6, 87, 749, 1088
BfmI CTRYAG 1 cut(s) 11
BfuI GTATCC 1 cut(s) 121
BisI GCNGC 4 cut(s) 67, 275, 973, 1129
BlsI GCNGC 4 cut(s) 68, 276, 974, 1130
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 3 cut(s) 161, 608, 845
Bme18I GGWCC 3 cut(s) 299, 911, 1058
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 299, 911, 1058, 1076
BmiI GGNNCC 3 cut(s) 300, 842, 1025
BmrFI CCNGG 3 cut(s) 161, 608, 845
BmsI GCATC 2 cut(s) 344, 751
BmtI GCTAGC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 827
Bpu14I TTCGAA 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 2 cut(s) 70, 787
BsaWI WCCGGW 2 cut(s) 218, 358
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 806, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 920
Bse1I ACTGG 3 cut(s) 136, 842, 964
Bse3DI GCAATG 2 cut(s) 511, 982
Bse8I GATNNNNATC 1 cut(s) 581
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 2 cut(s) 608, 845
BseDI CCNNGG 2 cut(s) 70, 787
BseGI GGATG 4 cut(s) 156, 582, 859, 1009
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 2 cut(s) 296, 920
BseMI GCAATG 2 cut(s) 511, 982
BseMII CTCAG 1 cut(s) 709
BseNI ACTGG 3 cut(s) 136, 842, 964
BseXI GCAGC 4 cut(s) 53, 261, 984, 1140
BsgI GTGCAG 1 cut(s) 802
BshFI GGCC 2 cut(s) 192, 1078
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 3 cut(s) 161, 219, 359
BslFI GGGAC 3 cut(s) 128, 312, 1013
BslI CCNNNNNNNGG 2 cut(s) 296, 920
BsmAI GTCTC 1 cut(s) 1100
BsmFI GGGAC 3 cut(s) 128, 312, 1013
BsnI GGCC 2 cut(s) 192, 1078
Bso31I GGTCTC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 780
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 5 cut(s) 260, 659, 903, 909, 1061
BspANI GGCC 2 cut(s) 192, 1078
BspCNI CTCAG 1 cut(s) 708
BspEI TCCGGA 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 300, 842, 1025
BspMAI CTGCAG 1 cut(s) 15
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 3 cut(s) 382, 1018, 1031
BspT104I TTCGAA 1 cut(s) 726
BspTNI GGTCTC 1 cut(s) 1100
BsrDI GCAATG 2 cut(s) 511, 982
BsrI ACTGG 3 cut(s) 136, 842, 964
BssECI CCNNGG 2 cut(s) 70, 787
BssMI GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
BssT1I CCWWGG 2 cut(s) 70, 787
Bst2UI CCWGG 2 cut(s) 608, 845
Bst4CI ACNGT 3 cut(s) 124, 429, 775
Bst6I CTCTTC 3 cut(s) 408, 444, 1103
BstBI TTCGAA 1 cut(s) 726
BstC8I GCNNGC 6 cut(s) 7, 11, 600, 671, 675, 780
BstDEI CTNAG 2 cut(s) 647, 695
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 4 cut(s) 156, 582, 859, 1009
BstHHI GCGC 1 cut(s) 766
BstKTI GATC 6 cut(s) 45, 377, 388, 859, 1026, 1156
BstMAI GTCTC 1 cut(s) 1100
BstMBI GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
BstMWI GCNNNNNNNGC 2 cut(s) 266, 683
BstNI CCWGG 2 cut(s) 608, 845
BstSCI CCNGG 3 cut(s) 159, 606, 843
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 4 cut(s) 53, 261, 984, 1140
BstX2I RGATCY 1 cut(s) 1023
BstYI RGATCY 1 cut(s) 1023
BsuI GTATCC 1 cut(s) 121
BsuRI GGCC 2 cut(s) 192, 1078
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 4 cut(s) 156, 582, 859, 1009
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 6 cut(s) 7, 11, 600, 671, 675, 780
CaiI CAGNNNCTG 1 cut(s) 613
CfoI GCGC 1 cut(s) 766
Cfr13I GGNCC 4 cut(s) 299, 911, 1058, 1076
CseI GACGC 2 cut(s) 231, 604
Csp6I GTAC 1 cut(s) 814
CviAII CATG 2 cut(s) 71, 1028
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 2 cut(s) 647, 695
DpnI GATC 6 cut(s) 44, 376, 387, 858, 1025, 1155
DpnII GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 408, 444, 1103
EarI CTCTTC 3 cut(s) 408, 444, 1103
Eco130I CCWWGG 2 cut(s) 70, 787
Eco31I GGTCTC 1 cut(s) 1100
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 3 cut(s) 299, 911, 1058
Eco88I CYCGRG 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 606, 843
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 70, 787
ErhI CCWWGG 2 cut(s) 70, 787
FaeI CATG 2 cut(s) 74, 1031
FaqI GGGAC 3 cut(s) 128, 312, 1013
FatI CATG 2 cut(s) 70, 1027
FauNDI CATATG 1 cut(s) 916
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 4 cut(s) 67, 275, 973, 1129
FokI GGATG 4 cut(s) 143, 589, 846, 996
Fsp4HI GCNGC 4 cut(s) 67, 275, 973, 1129
FspBI CTAG 4 cut(s) 6, 87, 749, 1088
GlaI GCGC 1 cut(s) 765
GluI GCNGC 4 cut(s) 67, 275, 973, 1129
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 2 cut(s) 192, 1078
HapII CCGG 3 cut(s) 161, 219, 359
HgaI GACGC 2 cut(s) 231, 604
HhaI GCGC 1 cut(s) 766
Hin1II CATG 2 cut(s) 74, 1031
Hin6I GCGC 1 cut(s) 764
HinP1I GCGC 1 cut(s) 764
HincII GTYRAC 2 cut(s) 328, 432
HindII GTYRAC 2 cut(s) 328, 432
HinfI GANTC 5 cut(s) 116, 324, 655, 888, 1001
HpaII CCGG 3 cut(s) 161, 219, 359
HphI GGTGA 2 cut(s) 142, 922
Hpy166II GTNNAC 5 cut(s) 250, 328, 432, 691, 930
Hpy188I TCNGA 5 cut(s) 232, 393, 475, 587, 698
Hpy188III TCNNGA 3 cut(s) 40, 359, 1051
Hpy8I GTNNAC 5 cut(s) 250, 328, 432, 691, 930
HpyAV CCTTC 5 cut(s) 708, 722, 1074, 1085, 1102
HpyCH4III ACNGT 3 cut(s) 124, 429, 775
HpyCH4V TGCA 9 cut(s) 13, 269, 335, 421, 544, 746, 819, 972, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 683
HpyF3I CTNAG 2 cut(s) 647, 695
Hsp92II CATG 2 cut(s) 74, 1031
HspAI GCGC 1 cut(s) 764
Kpn2I TCCGGA 1 cut(s) 358
Kzo9I GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
LmnI GCTCC 4 cut(s) 74, 344, 731, 846
Lsp1109I GCAGC 4 cut(s) 53, 261, 984, 1140
LweI GCATC 2 cut(s) 344, 751
MaeI CTAG 4 cut(s) 6, 87, 749, 1088
MaeIII GTNAC 1 cut(s) 521
MalI GATC 6 cut(s) 44, 376, 387, 858, 1025, 1155
MboI GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
MboII GAAGA 6 cut(s) 347, 380, 425, 461, 1059, 1090
MfeI CAATTG 1 cut(s) 398
MflI RGATCY 1 cut(s) 1023
MhlI GDGCHC 1 cut(s) 780
MluCI AATT 4 cut(s) 398, 440, 635, 826
MlyI GAGTC 3 cut(s) 110, 333, 995
MmeI TCCRAC 2 cut(s) 416, 1037
MroI TCCGGA 1 cut(s) 358
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 1 cut(s) 759
MspI CCGG 3 cut(s) 161, 219, 359
MspR9I CCNGG 3 cut(s) 161, 608, 845
MunI CAATTG 1 cut(s) 398
MvaI CCWGG 2 cut(s) 608, 845
MwoI GCNNNNNNNGC 2 cut(s) 266, 683
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 916
NdeII GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 74, 1031
NlaIV GGNNCC 3 cut(s) 300, 842, 1025
NspV TTCGAA 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 655, 888
PfoI TCCNGGA 2 cut(s) 159, 843
PkrI GCNGC 4 cut(s) 68, 276, 974, 1130
PleI GAGTC 3 cut(s) 110, 332, 995
PpsI GAGTC 3 cut(s) 110, 332, 995
Psp6I CCWGG 2 cut(s) 606, 843
PspGI CCWGG 2 cut(s) 606, 843
PspN4I GGNNCC 3 cut(s) 300, 842, 1025
PspPI GGNCC 4 cut(s) 299, 911, 1058, 1076
PstI CTGCAG 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 1023
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
RseI CAYNNNNRTG 1 cut(s) 759
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 4 cut(s) 67, 275, 973, 1129
Sau3AI GATC 6 cut(s) 42, 374, 385, 856, 1023, 1153
Sau96I GGNCC 4 cut(s) 299, 911, 1058, 1076
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 3 cut(s) 110, 333, 995
ScrFI CCNGG 3 cut(s) 161, 608, 845
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 2 cut(s) 344, 751
SfcI CTRYAG 1 cut(s) 11
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 3 cut(s) 299, 911, 1058
SmiMI CAYNNNNRTG 1 cut(s) 759
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 4 cut(s) 398, 440, 635, 826
SsiI CCGC 5 cut(s) 260, 659, 903, 909, 1061
SspMI CTAG 4 cut(s) 6, 87, 749, 1088
StyD4I CCNGG 3 cut(s) 159, 606, 843
StyI CCWWGG 2 cut(s) 70, 787
TaaI ACNGT 3 cut(s) 124, 429, 775
TaqI TCGA 2 cut(s) 119, 726
TasI AATT 4 cut(s) 398, 440, 635, 826
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 2 cut(s) 655, 888
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 1 cut(s) 486
TseI GCWGC 4 cut(s) 66, 274, 972, 1128
TspDTI ATGAA 2 cut(s) 94, 478
TspGWI ACGGA 1 cut(s) 900
TspRI CASTG 1 cut(s) 486
VpaK11BI GGWCC 3 cut(s) 299, 911, 1058
XapI RAATTY 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 4 cut(s) 6, 87, 749, 1088
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.