Rh2AG103500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017214
Physical Location & Seq
Reverse (-)
8896192 .. 8903433
7242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG103500.1

Sequence Viewer

Length: 1170 bp
ATGGCTAGCCTGCAGATTTCTCTCTCGGGTCAGCTAATCATAATCTGTGTGATGATGATGAGTGGCTGCCATGGAGCATTTGCTACTAGTGTTGCCTTGTTCATTTTCGGGGACTCGACTGTGGATACTGGCAACAACAACTACATCACCACCGTCCCGGAAAACCAAGCACATTACAAGCCCTACGGCCAGAATGGTTTCTTTGACCACCCCACCGGACGCTTCTCCGATGGTCGTGTCATTGTAGACTACATAGCGGAATATGCAAAGCTGCCAATAATCCCTCCATTTTTGGGACCATCTGATGACTACACAAATGGAGTCAACTTTGCATCGGGTGGTGCTGGTGTTCTTCCGGAAACCAATCAAGGATCGGCGATTGATCTTCCGACACAATTGAGATACTTTGAAGAGGTGCAAAAACGGTTCACAAAAAAATTGGGAGAAGAGAAAGCAAAGGAAGTCATATCAGAGGCAGTGTATTTCATTAGTATTGGAAGCAATGACTATATGGGCGGTTACCTAGGTAATCCAAAAATGCAGGAACAATATAACCCTGAACAATATGTGGGGATGGTCATCGGAAACTTGACGCAGGCAATCCAAGTGCTGTATGAAAAAGGGGGTAGAAAATTTGGTTTCCTAAGATTGACTCCGCTTGGTTGCTTGCCTGCCTTTAGAGCTGTGAACCTCAGAGCCACGAAAGGAGGAAGGGGCTGTTTCGAAGGAGCTTCTGCCCTTGCACTAGCTCATAATGATGCGCTTAATACTGTGCTCGCAAACCTTGGATATGTTCTCAAAGAGTTCAAGTACTGCAGCTCCAATTTCTATGACTGGCTCCAGGAAAGAATAGATCATCCTTACAAATATGGTTTCAAGGATGGAATCAATGCTTGTTGCGGAAGCGGACCATATGGAGGTTCGTTCACCTGTGGAGGCACAAAGGAGGATAAAGATTACCAGTTATGTAGCAATGCTGATGATTATATATGGTGGGACTCCTTCCATCCAACAGAGAGGATCCATGAGCAACTTGCAAAGGCTCTATGGAATGGACCGCCTTCTTCTGTCGGGCCATACAACCTAGAAGGTCTCTTCTTTGAGAAGGAGAAGCTCACCATTGGCAGCATAGTTGATAAACCAGAAGCAGATCAGTTTCAGTCCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

42.92

Weight (kDa)

5.26

Isoelectric Point (pI)

27.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 349 7.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09430
malus_domestica MD02G1102600.v1.1
prunus_persica Prupe.7G191700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095981
rosa_laevigata RLG00000016614
rosa_multiflora Rmu_sc0002497.1_g000009
rosa_roxburghii Rroxscaffold_2G00145890
rosa_rugosa Rorug02G0055600
rosa_samantha Rh2AG103500 Rh2BG105000 Rh2CG107500 Rh2DG105400
rosa_wichuraiana Rw2G007890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 246
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 6 cut(s) 257, 516, 656, 900, 906, 1058
AclWI GGATC 3 cut(s) 379, 1015, 1028
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 632
AfaI GTAC 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 293, 917
AgsI TTSAA 3 cut(s) 410, 808, 877
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 840
AluBI AGCT 7 cut(s) 34, 271, 683, 731, 749, 819, 1114
AluI AGCT 7 cut(s) 34, 271, 683, 731, 749, 819, 1114
Alw21I GWGCWC 1 cut(s) 777
Alw26I GTCTC 1 cut(s) 1097
AlwI GGATC 3 cut(s) 379, 1015, 1028
Ama87I CYCGRG 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 2 cut(s) 187, 1073
ApeKI GCWGC 4 cut(s) 66, 271, 816, 1125
ApoI RAATTY 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 197
AspA2I CCTAGG 1 cut(s) 523
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 4 cut(s) 296, 908, 1055, 1073
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 139, 919, 1108
AsuII TTCGAA 1 cut(s) 723
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 3 cut(s) 296, 908, 1055
AvrII CCTAGG 1 cut(s) 523
BamHI GGATCC 1 cut(s) 1020
Bbv12I GWGCWC 1 cut(s) 777
BbvI GCAGC 4 cut(s) 53, 258, 828, 1137
BccI CCATC 5 cut(s) 224, 307, 568, 875, 1014
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 842
BciVI GTATCC 1 cut(s) 118
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 1097
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 5 cut(s) 6, 87, 524, 746, 1085
BfmI CTRYAG 2 cut(s) 11, 814
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 4 cut(s) 67, 272, 817, 1126
BlnI CCTAGG 1 cut(s) 523
BlsI GCNGC 4 cut(s) 68, 273, 818, 1127
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 2 cut(s) 158, 842
Bme18I GGWCC 3 cut(s) 296, 908, 1055
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 296, 908, 1055, 1073
BmiI GGNNCC 3 cut(s) 297, 839, 1022
BmrFI CCNGG 2 cut(s) 158, 842
BmsI GCATC 2 cut(s) 341, 748
BmtI GCTAGC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 824
Bpu14I TTCGAA 1 cut(s) 723
BpuMI CCSGG 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 578
BsaI GGTCTC 1 cut(s) 1097
BsaJI CCNNGG 3 cut(s) 70, 523, 784
BsaWI WCCGGW 2 cut(s) 215, 355
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 917
Bse1I ACTGG 3 cut(s) 133, 839, 961
Bse3DI GCAATG 2 cut(s) 508, 979
Bse8I GATNNNNATC 1 cut(s) 578
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 3 cut(s) 70, 523, 784
BseGI GGATG 4 cut(s) 579, 856, 886, 1006
BseJI GATNNNNATC 1 cut(s) 578
BseLI CCNNNNNNNGG 2 cut(s) 293, 917
BseMI GCAATG 2 cut(s) 508, 979
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 3 cut(s) 133, 839, 961
BseXI GCAGC 4 cut(s) 53, 258, 828, 1137
BshFI GGCC 2 cut(s) 189, 1075
BsiHKAI GWGCWC 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 3 cut(s) 158, 216, 356
BslFI GGGAC 4 cut(s) 125, 140, 309, 1010
BslI CCNNNNNNNGG 2 cut(s) 293, 917
BsmAI GTCTC 1 cut(s) 1097
BsmFI GGGAC 4 cut(s) 125, 140, 309, 1010
BsnI GGCC 2 cut(s) 189, 1075
Bso31I GGTCTC 1 cut(s) 1097
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 723
Bsp1286I GDGCHC 1 cut(s) 777
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 5 cut(s) 371, 382, 853, 1020, 1150
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 6 cut(s) 257, 516, 656, 900, 906, 1058
BspANI GGCC 2 cut(s) 189, 1075
BspCNI CTCAG 1 cut(s) 705
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 3 cut(s) 297, 839, 1022
BspMAI CTGCAG 2 cut(s) 15, 818
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 3 cut(s) 379, 1015, 1028
BspT104I TTCGAA 1 cut(s) 723
BspTNI GGTCTC 1 cut(s) 1097
BsrDI GCAATG 2 cut(s) 508, 979
BsrI ACTGG 3 cut(s) 133, 839, 961
BssECI CCNNGG 3 cut(s) 70, 523, 784
BssMI GATC 5 cut(s) 371, 382, 853, 1020, 1150
BssT1I CCWWGG 3 cut(s) 70, 523, 784
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 4 cut(s) 121, 154, 426, 772
Bst6I CTCTTC 3 cut(s) 405, 441, 1100
BstBI TTCGAA 1 cut(s) 723
BstC8I GCNNGC 6 cut(s) 7, 11, 597, 668, 672, 777
BstDEI CTNAG 2 cut(s) 644, 692
BstDSI CCRYGG 1 cut(s) 70
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 4 cut(s) 579, 856, 886, 1006
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 5 cut(s) 374, 385, 856, 1023, 1153
BstMAI GTCTC 1 cut(s) 1097
BstMBI GATC 5 cut(s) 371, 382, 853, 1020, 1150
BstMWI GCNNNNNNNGC 2 cut(s) 263, 680
BstNI CCWGG 1 cut(s) 842
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 156, 840
BstSFI CTRYAG 2 cut(s) 11, 814
BstV1I GCAGC 4 cut(s) 53, 258, 828, 1137
BstX2I RGATCY 1 cut(s) 1020
BstYI RGATCY 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 2 cut(s) 189, 1075
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 4 cut(s) 579, 856, 886, 1006
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 6 cut(s) 7, 11, 597, 668, 672, 777
CfoI GCGC 1 cut(s) 763
Cfr13I GGNCC 4 cut(s) 296, 908, 1055, 1073
CseI GACGC 2 cut(s) 228, 601
Csp6I GTAC 1 cut(s) 811
CviAII CATG 2 cut(s) 71, 1025
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 2 cut(s) 644, 692
DpnI GATC 5 cut(s) 373, 384, 855, 1022, 1152
DpnII GATC 5 cut(s) 371, 382, 853, 1020, 1150
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 3 cut(s) 405, 441, 1100
EarI CTCTTC 3 cut(s) 405, 441, 1100
Eco130I CCWWGG 3 cut(s) 70, 523, 784
Eco31I GGTCTC 1 cut(s) 1097
Eco47I GGWCC 3 cut(s) 296, 908, 1055
Eco88I CYCGRG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 840
EcoT14I CCWWGG 3 cut(s) 70, 523, 784
ErhI CCWWGG 3 cut(s) 70, 523, 784
FaeI CATG 2 cut(s) 74, 1028
FaqI GGGAC 4 cut(s) 125, 140, 309, 1010
FatI CATG 2 cut(s) 70, 1024
FauNDI CATATG 1 cut(s) 913
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 67, 272, 817, 1126
FokI GGATG 4 cut(s) 586, 843, 893, 993
Fsp4HI GCNGC 4 cut(s) 67, 272, 817, 1126
FspBI CTAG 5 cut(s) 6, 87, 524, 746, 1085
GlaI GCGC 1 cut(s) 762
GluI GCNGC 4 cut(s) 67, 272, 817, 1126
GsuI CTGGAG 1 cut(s) 824
HaeIII GGCC 2 cut(s) 189, 1075
HapII CCGG 3 cut(s) 158, 216, 356
HgaI GACGC 2 cut(s) 228, 601
HhaI GCGC 1 cut(s) 763
Hin1II CATG 2 cut(s) 74, 1028
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 5 cut(s) 113, 321, 652, 885, 998
HpaII CCGG 3 cut(s) 158, 216, 356
HphI GGTGA 3 cut(s) 139, 919, 1108
Hpy166II GTNNAC 5 cut(s) 247, 325, 429, 688, 927
Hpy188I TCNGA 6 cut(s) 229, 304, 390, 472, 584, 695
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 5 cut(s) 247, 325, 429, 688, 927
HpyAV CCTTC 6 cut(s) 705, 719, 1012, 1071, 1082, 1099
HpyCH4III ACNGT 4 cut(s) 121, 154, 426, 772
HpyCH4V TGCA 8 cut(s) 13, 266, 332, 418, 541, 743, 816, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 680
HpyF3I CTNAG 2 cut(s) 644, 692
Hsp92II CATG 2 cut(s) 74, 1028
HspAI GCGC 1 cut(s) 761
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 5 cut(s) 371, 382, 853, 1020, 1150
LmnI GCTCC 4 cut(s) 74, 728, 824, 843
Lsp1109I GCAGC 4 cut(s) 53, 258, 828, 1137
LweI GCATC 2 cut(s) 341, 748
MaeI CTAG 5 cut(s) 6, 87, 524, 746, 1085
MaeIII GTNAC 1 cut(s) 518
MalI GATC 5 cut(s) 373, 384, 855, 1022, 1152
MboI GATC 5 cut(s) 371, 382, 853, 1020, 1150
MboII GAAGA 6 cut(s) 344, 377, 422, 458, 1056, 1087
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 1 cut(s) 1020
MhlI GDGCHC 1 cut(s) 777
MluCI AATT 4 cut(s) 395, 437, 632, 823
MlyI GAGTC 4 cut(s) 107, 330, 646, 992
MmeI TCCRAC 2 cut(s) 413, 1034
MnlI CCTC 9 cut(s) 294, 406, 466, 701, 701, 911, 929, 940, 1011
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 756
MspI CCGG 3 cut(s) 158, 216, 356
MspR9I CCNGG 2 cut(s) 158, 842
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 842
MwoI GCNNNNNNNGC 2 cut(s) 263, 680
NciI CCSGG 1 cut(s) 158
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 913
NdeII GATC 5 cut(s) 371, 382, 853, 1020, 1150
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 74, 1028
NlaIV GGNNCC 3 cut(s) 297, 839, 1022
NspV TTCGAA 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 885
PfoI TCCNGGA 2 cut(s) 156, 840
PkrI GCNGC 4 cut(s) 68, 273, 818, 1127
PleI GAGTC 4 cut(s) 107, 329, 646, 992
PpsI GAGTC 4 cut(s) 107, 329, 646, 992
Psp6I CCWGG 1 cut(s) 840
PspEI GGTNACC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 3 cut(s) 297, 839, 1022
PspPI GGNCC 4 cut(s) 296, 908, 1055, 1073
PstI CTGCAG 2 cut(s) 15, 818
PsuI RGATCY 1 cut(s) 1020
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 756
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 4 cut(s) 67, 272, 817, 1126
Sau3AI GATC 5 cut(s) 371, 382, 853, 1020, 1150
Sau96I GGNCC 4 cut(s) 296, 908, 1055, 1073
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 4 cut(s) 107, 330, 646, 992
ScrFI CCNGG 2 cut(s) 158, 842
SduI GDGCHC 1 cut(s) 777
SfaNI GCATC 2 cut(s) 341, 748
SfcI CTRYAG 2 cut(s) 11, 814
SfuI TTCGAA 1 cut(s) 723
SinI GGWCC 3 cut(s) 296, 908, 1055
SmiMI CAYNNNNRTG 1 cut(s) 756
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 4 cut(s) 395, 437, 632, 823
SsiI CCGC 6 cut(s) 257, 516, 656, 900, 906, 1058
SspMI CTAG 5 cut(s) 6, 87, 524, 746, 1085
StyD4I CCNGG 2 cut(s) 156, 840
StyI CCWWGG 3 cut(s) 70, 523, 784
TaaI ACNGT 4 cut(s) 121, 154, 426, 772
TaqI TCGA 2 cut(s) 116, 723
TasI AATT 4 cut(s) 395, 437, 632, 823
TatI WGTACW 1 cut(s) 810
TfiI GAWTC 1 cut(s) 885
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 1 cut(s) 483
TseI GCWGC 4 cut(s) 66, 271, 816, 1125
TspDTI ATGAA 3 cut(s) 91, 475, 630
TspRI CASTG 1 cut(s) 483
VpaK11BI GGWCC 3 cut(s) 296, 908, 1055
XapI RAATTY 1 cut(s) 632
XmaJI CCTAGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 5 cut(s) 6, 87, 524, 746, 1085
ZrmI AGTACT 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.