MD02G1107500.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016727
Physical Location & Seq
Reverse (-)
8675682 .. 8679919
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1107500.v1.1.491

Sequence Viewer

Length: 2070 bp
ATGGAGTCTTTTTGTTGTACTGGGAGAATCAGCAGCGTTCTTCTTCTGTTCTTATGTGTTCTTGCTGCTGAAATTAGCATTTGCCTTTCATCCAAGGTGTATGTAGTTTATATGGGAAGCAAAAATGGTGAGGACCCAGATGAAATTTTGACACAAAACCATGAAATGCTTGCTTTTGTGCATACTGGAAGCATGGAGGAAGCTCGGGCATCTCACCTTTACAGTTACAGACATGGCTTCAGAGGCTTTGCAGCCAAGTTAACTGACCTCCAAGCTTTCCAAATTTCCAAGATGCCGGGAGTTGTTTCGGTTTTCCCCAATTCGAAAAGAAAGTTGCACACCACACATTCCTGGGATTTCATGGGCCTTTTGGGTGAAGAAACCATGGAGATTATTGGGTATTCCACCAAGAACCAAGTTGATGTCATTGTTGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCAAGTTTTGATGATGCCAACATGCCTCCTGTGCCAGCTAGATGGAAGGGGCGTTGTGAATCAGGAGAAAAATTCACCGCTTTAACTTGCAACAGGAAAGTGATTGGCGCTCGATACTATAATTCTGGTTATGAAGCCGAGGAAGAGTCAACAACTACAGTGTCATTCAGATCACCAAGGGACAGCTCAGGTCATGGCAGTCACACAGCCTCTATAGCTGCTGGTCGGTACGTGTTAAACATGACTTATAAGGGATTGGCGGCTGGAGGGGCAAGAGGAGGGGCACCAATGGCTAGGATTGCAGTATACAAGACATGTTGGAACTCTGGTTGTTATGACGTTGACTTACTAGCGGCCTTTGATGATGCTATCAGAGACGGGGTTGACATCATGTCTGTGTCTCTAGGTCCTGATGCTCCTCAGGGAGACTATTTCAGTGACGCCATTTCTATCGGATCATTTCATGCTGCTAGGCATGGAATTCTAGTGGTTGCTTCTTCTGGAAATGAAGGAAACCCAGGTTCTGCAACAAATTTAGCCCCATGGATGCTCACTGTTGGTGCAAGTTCAACAGATAGGGATTTCACTTCTGATATCATACTAGAAAATGGTGCTAATTTCACGGGTGAAAGTCTCAGTGTCCTCGAAATGAAAGCCTCAGCAAGAATTATTTCTGCCTCTGAAGCCAATGCAGGGTATTTTACTCCGTATCAATCCAGTTACTGTTTAGAAAGTTCCTTGAATAGAACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGACACGCTGAGAGTTCAACGGAGTCAAAGCTCGCTAAAAGTATGCTAGTGGAAAAAGCTGGTGGTGTTGGGATGGTTCTCATTGATGATGCAGATAAGGATATTGCTGTCCCATTTGTTATCCCTTCTGCTATCGTTGGACAGAGGATAGGGCATCAAATTTTATCTTACATTATACGTGCACGAAAACCCAAGTCGCGAATTTTGCCTGCAAAAACTATACTTGGACTGAAACCTGCACCTCGTGTTACAGCATTTTCTTCAAAAGGCCCCAATTCTTTGACACCAGAGATTTTGAAGCCCGATGTCACAGCTCCTGGACTAAATATACTAGCATCTTGGTCCCCAGCAGCAGGGAATAAGCTGTTCAACATTCTTTCTGGTACTTCCATGGCTTGCCCACATGTAACAGGAATCGCCGCCTTGATCAAGGCCGTTTATCCATCATGGTCCCCAGCAGCCATTAGATCCGCAATCATGATTACAGCTACACTTCTGGATAAGAACTCCAAACCCATCCTAGTGGACCCGGAAGGAAGAAGGGGCAACGCATTCGATTATGGCTCAGGCTTTGTGAACCCGAAAAGAGTCCTTGACCCAGGTCTTGTCTACGACGCACATTCAGCAGACTACGTAGAATTCCTTTGTTCAGTTGGTTATGACAAGAAGGCGGTCCACCAAATCACACGAGACAATAGTACATGTAAGCAGGCATTCCGAACAGCATCTGACCTGAACTATCCATCTATCACAGTGCCATATCGTGTGCGCAACTTCTCGGTAACTAGAACAGTGACCAATGTTGGGAAGGCCAAAGAGTATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

690

Amino Acids

74.28

Weight (kDa)

8.4

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 1.3e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 585 5e-48 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc16I TGCGCA 1 cut(s) 2012
Acc36I ACCTGC 1 cut(s) 1486
AccB1I GGYRCC 1 cut(s) 748
AccI GTMKAC 3 cut(s) 771, 1243, 1851
AccII CGCG 1 cut(s) 1441
AciI CCGC 6 cut(s) 543, 725, 818, 1662, 1713, 1913
AclWI GGATC 2 cut(s) 928, 1704
AcsI RAATTY 9 cut(s) 144, 282, 450, 536, 945, 997, 1401, 1443, 1880
AcuI CTGAAG 2 cut(s) 223, 1167
AcyI GRCGYC 1 cut(s) 906
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 4 cut(s) 19, 695, 1627, 1942
AfiI CCNNNNNNNGG 3 cut(s) 1158, 1595, 2046
AflIII ACRYGT 4 cut(s) 696, 779, 1645, 1943
AgsI TTSAA 6 cut(s) 1035, 1207, 1260, 1506, 1540, 1612
AhdI GACNNNNNGTC 1 cut(s) 856
AjnI CCWGG 4 cut(s) 350, 982, 1558, 1840
Alw21I GWGCWC 1 cut(s) 1426
Alw26I GTCTC 5 cut(s) 834, 870, 885, 1103, 1926
Alw44I GTGCAC 1 cut(s) 1422
AlwI GGATC 2 cut(s) 928, 1704
AlwNI CAGNNNCTG 4 cut(s) 989, 1188, 1559, 1970
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 7 cut(s) 364, 455, 819, 1218, 1510, 1674, 2052
ApaLI GTGCAC 1 cut(s) 1422
ApeKI GCWGC 7 cut(s) 33, 65, 251, 683, 932, 1592, 1700
ApoI RAATTY 9 cut(s) 144, 282, 450, 536, 945, 997, 1401, 1443, 1880
Asp700I GAANNNNTTC 1 cut(s) 1135
AspLEI GCGC 2 cut(s) 575, 2013
AspS9I GGNCC 8 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768, 1915
AsuC2I CCSGG 2 cut(s) 297, 1772
AsuHPI GGTGA 6 cut(s) 140, 206, 386, 532, 630, 1103
AsuII TTCGAA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 6 cut(s) 133, 872, 1584, 1692, 1768, 1915
AxyI CCTNAGG 1 cut(s) 885
BaeGI GKGCMC 2 cut(s) 751, 1426
BanI GGYRCC 1 cut(s) 748
BauI CACGAG 2 cut(s) 1485, 1929
Bbv12I GWGCWC 1 cut(s) 1426
BbvCI CCTCAGC 1 cut(s) 1123
BbvI GCAGC 7 cut(s) 45, 52, 263, 670, 919, 1604, 1712
BccI CCATC 5 cut(s) 501, 1309, 1693, 1766, 1993
BceAI ACGGC 1 cut(s) 1661
BcgI CGANNNNNNTGC 2 cut(s) 1777, 1811
BciT130I CCWGG 4 cut(s) 352, 984, 1560, 1842
BclI TGATCA 1 cut(s) 1668
BcnI CCSGG 2 cut(s) 297, 1772
BcoDI GTCTC 5 cut(s) 834, 870, 885, 1103, 1926
BfmI CTRYAG 3 cut(s) 621, 678, 2066
BfoI RGCGCY 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 1486
Bme1390I CCNGG 6 cut(s) 297, 352, 984, 1560, 1772, 1842
Bme18I GGWCC 6 cut(s) 133, 872, 1584, 1692, 1768, 1915
BmeRI GACNNNNNGTC 1 cut(s) 856
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 8 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768, 1915
BmiI GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
BmrFI CCNGG 6 cut(s) 297, 352, 984, 1560, 1772, 1842
BmrI ACTGGG 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 30
BoxI GACNNNNGTC 1 cut(s) 1842
BpmI CTGGAG 1 cut(s) 750
Bpu10I CCTNAGC 3 cut(s) 652, 1123, 1807
Bpu14I TTCGAA 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 297, 1772
BsaAI YACGTR 3 cut(s) 697, 1421, 1876
BsaHI GRCGYC 1 cut(s) 906
Bsc4I CCNNNNNNNGG 3 cut(s) 1158, 1595, 2046
Bse1I ACTGG 4 cut(s) 25, 190, 451, 1182
Bse21I CCTNAGG 1 cut(s) 885
BseBI CCWGG 4 cut(s) 352, 984, 1560, 1842
BseGI GGATG 4 cut(s) 89, 1017, 1320, 1758
BseLI CCNNNNNNNGG 3 cut(s) 1158, 1595, 2046
BseMII CTCAG 6 cut(s) 666, 899, 1114, 1137, 1242, 1821
BseNI ACTGG 4 cut(s) 25, 190, 451, 1182
BseRI GAGGAG 2 cut(s) 756, 873
BseSI GKGCMC 2 cut(s) 751, 1426
BseXI GCAGC 7 cut(s) 45, 52, 263, 670, 919, 1604, 1712
BseYI CCCAGC 2 cut(s) 1588, 1696
BsgI GTGCAG 1 cut(s) 1464
Bsh1236I CGCG 1 cut(s) 1441
BshFI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1676, 2054
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 1426
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 296, 1772
BslFI GGGAC 4 cut(s) 659, 1337, 1570, 1678
BslI CCNNNNNNNGG 3 cut(s) 1158, 1595, 2046
BsmAI GTCTC 5 cut(s) 834, 870, 885, 1103, 1926
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 4 cut(s) 659, 1337, 1570, 1678
BsmI GAATGC 2 cut(s) 1793, 1955
BsnI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1676, 2054
BsoBI CYCGRG 1 cut(s) 204
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 2 cut(s) 751, 1426
Bsp143I GATC 4 cut(s) 635, 920, 1668, 1709
Bsp19I CCATGG 3 cut(s) 384, 1007, 1632
Bsp68I TCGCGA 1 cut(s) 1441
BspACI CCGC 6 cut(s) 543, 725, 818, 1662, 1713, 1913
BspANI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1676, 2054
BspCNI CTCAG 6 cut(s) 665, 898, 1113, 1136, 1243, 1820
BspFNI CGCG 1 cut(s) 1441
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
BspMI ACCTGC 1 cut(s) 1486
BspPI GGATC 2 cut(s) 928, 1704
BspT104I TTCGAA 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 748
BsrI ACTGG 4 cut(s) 25, 190, 451, 1182
BssMI GATC 4 cut(s) 635, 920, 1668, 1709
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 906
BssSI CACGAG 2 cut(s) 1485, 1929
BssT1I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Bst1107I GTATAC 1 cut(s) 772
Bst2BI CACGAG 2 cut(s) 1485, 1929
Bst2UI CCWGG 4 cut(s) 352, 984, 1560, 1842
Bst4CI ACNGT 6 cut(s) 224, 625, 1021, 1190, 1996, 2035
Bst6I CTCTTC 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 906
BstBAI YACGTR 3 cut(s) 697, 1421, 1876
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 6 cut(s) 171, 501, 1275, 1452, 1639, 1953
BstDEI CTNAG 6 cut(s) 652, 885, 1100, 1123, 1251, 1807
BstDSI CCRYGG 3 cut(s) 384, 1007, 1632
BstF5I GGATG 4 cut(s) 89, 1017, 1320, 1758
BstFNI CGCG 1 cut(s) 1441
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 2 cut(s) 575, 2013
BstKTI GATC 4 cut(s) 638, 923, 1671, 1712
BstMAI GTCTC 5 cut(s) 834, 870, 885, 1103, 1926
BstMBI GATC 4 cut(s) 635, 920, 1668, 1709
BstMWI GCNNNNNNNGC 9 cut(s) 243, 496, 680, 734, 755, 764, 1148, 1447, 1865
BstNI CCWGG 4 cut(s) 352, 984, 1560, 1842
BstNSI RCATGY 4 cut(s) 490, 783, 1649, 1947
BstPAI GACNNNNGTC 1 cut(s) 1842
BstSCI CCNGG 6 cut(s) 295, 350, 982, 1558, 1770, 1840
BstSFI CTRYAG 3 cut(s) 621, 678, 2066
BstSLI GKGCMC 2 cut(s) 751, 1426
BstSNI TACGTA 1 cut(s) 1876
BstUI CGCG 1 cut(s) 1441
BstV1I GCAGC 7 cut(s) 45, 52, 263, 670, 919, 1604, 1712
BstX2I RGATCY 1 cut(s) 1709
BstXI CCANNNNNNTGG 2 cut(s) 507, 1765
BstYI RGATCY 1 cut(s) 1709
BstZ17I GTATAC 1 cut(s) 772
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1676, 2054
BtgI CCRYGG 3 cut(s) 384, 1007, 1632
BtsCI GGATG 4 cut(s) 89, 1017, 1320, 1758
BtsIMutI CAGTG 6 cut(s) 630, 907, 1017, 1108, 2001, 2040
BtuMI TCGCGA 1 cut(s) 1441
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 6 cut(s) 171, 501, 1275, 1452, 1639, 1953
CaiI CAGNNNCTG 4 cut(s) 989, 1188, 1559, 1970
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 2 cut(s) 575, 2013
Cfr13I GGNCC 8 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768, 1915
CseI GACGC 2 cut(s) 914, 1865
Csp6I GTAC 4 cut(s) 18, 694, 1626, 1941
CviQI GTAC 4 cut(s) 18, 694, 1626, 1941
DdeI CTNAG 6 cut(s) 652, 885, 1100, 1123, 1251, 1807
DpnI GATC 4 cut(s) 637, 922, 1670, 1711
DpnII GATC 4 cut(s) 635, 920, 1668, 1709
DraIII CACNNNGTG 1 cut(s) 1487
DriI GACNNNNNGTC 1 cut(s) 856
Eam1104I CTCTTC 1 cut(s) 603
Eam1105I GACNNNNNGTC 1 cut(s) 856
EarI CTCTTC 1 cut(s) 603
Eco105I TACGTA 1 cut(s) 1876
Eco130I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 6 cut(s) 133, 872, 1584, 1692, 1768, 1915
Eco57I CTGAAG 2 cut(s) 223, 1167
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 204
EcoO109I RGGNCCY 3 cut(s) 133, 872, 1511
EcoRI GAATTC 2 cut(s) 945, 1880
EcoRII CCWGG 4 cut(s) 350, 982, 1558, 1840
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
ErhI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Esp3I CGTCTC 1 cut(s) 834
FaqI GGGAC 4 cut(s) 659, 1337, 1570, 1678
FbaI TGATCA 1 cut(s) 1668
FblI GTMKAC 3 cut(s) 771, 1243, 1851
FokI GGATG 4 cut(s) 76, 1024, 1327, 1745
FspI TGCGCA 1 cut(s) 2012
GlaI GCGC 2 cut(s) 574, 2012
GsaI CCCAGC 2 cut(s) 1592, 1700
GsuI CTGGAG 1 cut(s) 750
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1676, 2054
HapII CCGG 2 cut(s) 296, 1772
HgaI GACGC 2 cut(s) 914, 1865
HhaI GCGC 2 cut(s) 575, 2013
Hin1I GRCGYC 1 cut(s) 906
Hin6I GCGC 2 cut(s) 573, 2011
HinP1I GCGC 2 cut(s) 573, 2011
HincII GTYRAC 5 cut(s) 261, 615, 808, 850, 1244
HindII GTYRAC 5 cut(s) 261, 615, 808, 850, 1244
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 8 cut(s) 5, 27, 461, 524, 611, 1265, 1656, 1830
HpaI GTTAAC 1 cut(s) 261
HpaII CCGG 2 cut(s) 296, 1772
HphI GGTGA 6 cut(s) 140, 206, 386, 532, 630, 1103
Hpy188I TCNGA 8 cut(s) 242, 635, 839, 920, 1057, 1147, 1961, 1972
Hpy188III TCNNGA 6 cut(s) 528, 875, 966, 1440, 1720, 1739
Hpy99I CGWCG 1 cut(s) 1859
HpyAV CCTTC 8 cut(s) 505, 968, 1222, 1377, 1769, 1776, 1903, 2044
HpyCH4III ACNGT 6 cut(s) 224, 625, 1021, 1190, 1996, 2035
HpyCH4IV ACGT 4 cut(s) 696, 804, 1420, 1875
HpyF10VI GCNNNNNNNGC 9 cut(s) 243, 496, 680, 734, 755, 764, 1148, 1447, 1865
HpyF3I CTNAG 6 cut(s) 652, 885, 1100, 1123, 1251, 1807
HpySE526I ACGT 4 cut(s) 696, 804, 1420, 1875
Hsp92I GRCGYC 1 cut(s) 906
HspAI GCGC 2 cut(s) 573, 2011
Ksp22I TGATCA 1 cut(s) 1668
KspAI GTTAAC 1 cut(s) 261
Kzo9I GATC 4 cut(s) 635, 920, 1668, 1709
LmnI GCTCC 2 cut(s) 886, 1561
Lsp1109I GCAGC 7 cut(s) 45, 52, 263, 670, 919, 1604, 1712
MaeII ACGT 4 cut(s) 696, 804, 1420, 1875
MaeIII GTNAC 9 cut(s) 224, 665, 902, 1184, 1489, 1549, 1648, 2023, 2035
MalI GATC 4 cut(s) 637, 922, 1670, 1711
MboI GATC 4 cut(s) 635, 920, 1668, 1709
MboII GAAGA 7 cut(s) 32, 35, 389, 620, 954, 1494, 1791
MflI RGATCY 1 cut(s) 1709
MhlI GDGCHC 2 cut(s) 751, 1426
MlyI GAGTC 4 cut(s) 14, 620, 1274, 1839
MmeI TCCRAC 2 cut(s) 764, 1360
MroXI GAANNNNTTC 1 cut(s) 1135
MseI TTAA 3 cut(s) 260, 548, 701
MslI CAYNNNNRTG 2 cut(s) 860, 1763
MspI CCGG 2 cut(s) 296, 1772
MspR9I CCNGG 6 cut(s) 297, 352, 984, 1560, 1772, 1842
Mva1269I GAATGC 2 cut(s) 1793, 1955
MvaI CCWGG 4 cut(s) 352, 984, 1560, 1842
MvnI CGCG 1 cut(s) 1441
MwoI GCNNNNNNNGC 9 cut(s) 243, 496, 680, 734, 755, 764, 1148, 1447, 1865
NciI CCSGG 2 cut(s) 297, 1772
NcoI CCATGG 3 cut(s) 384, 1007, 1632
NdeII GATC 4 cut(s) 635, 920, 1668, 1709
NlaIV GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 4 cut(s) 665, 902, 1549, 2035
NruI TCGCGA 1 cut(s) 1441
NsbI TGCGCA 1 cut(s) 2012
NspI RCATGY 4 cut(s) 490, 783, 1649, 1947
NspV TTCGAA 1 cut(s) 323
PagI TCATGA 1 cut(s) 1719
PciI ACATGT 3 cut(s) 779, 1645, 1943
PctI GAATGC 2 cut(s) 1793, 1955
PdmI GAANNNNTTC 1 cut(s) 1135
PfeI GAWTC 4 cut(s) 27, 461, 524, 1656
PfoI TCCNGGA 1 cut(s) 1558
PleI GAGTC 4 cut(s) 13, 619, 1273, 1838
PpsI GAGTC 4 cut(s) 13, 619, 1273, 1838
Ppu21I YACGTR 3 cut(s) 697, 1421, 1876
PpuMI RGGWCCY 2 cut(s) 133, 872
PscI ACATGT 3 cut(s) 779, 1645, 1943
PshAI GACNNNNGTC 1 cut(s) 1842
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 2 cut(s) 133, 872
Psp6I CCWGG 4 cut(s) 350, 982, 1558, 1840
PspFI CCCAGC 2 cut(s) 1588, 1696
PspGI CCWGG 4 cut(s) 350, 982, 1558, 1840
PspN4I GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
PspPI GGNCC 8 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768, 1915
PspPPI RGGWCCY 2 cut(s) 133, 872
PstNI CAGNNNCTG 4 cut(s) 989, 1188, 1559, 1970
PsuI RGATCY 1 cut(s) 1709
RruI TCGCGA 1 cut(s) 1441
RsaI GTAC 4 cut(s) 19, 695, 1627, 1942
RsaNI GTAC 4 cut(s) 18, 694, 1626, 1941
RseI CAYNNNNRTG 2 cut(s) 860, 1763
SalI GTCGAC 1 cut(s) 1242
SaqAI TTAA 3 cut(s) 260, 548, 701
Sau3AI GATC 4 cut(s) 635, 920, 1668, 1709
Sau96I GGNCC 8 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768, 1915
SchI GAGTC 4 cut(s) 14, 620, 1274, 1839
ScrFI CCNGG 6 cut(s) 297, 352, 984, 1560, 1772, 1842
SduI GDGCHC 2 cut(s) 751, 1426
SfcI CTRYAG 3 cut(s) 621, 678, 2066
SfuI TTCGAA 1 cut(s) 323
SinI GGWCC 6 cut(s) 133, 872, 1584, 1692, 1768, 1915
SmiMI CAYNNNNRTG 2 cut(s) 860, 1763
SnaBI TACGTA 1 cut(s) 1876
SsiI CCGC 6 cut(s) 543, 725, 818, 1662, 1713, 1913
StyD4I CCNGG 6 cut(s) 295, 350, 982, 1558, 1770, 1840
StyI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
TaaI ACNGT 6 cut(s) 224, 625, 1021, 1190, 1996, 2035
TaiI ACGT 4 cut(s) 699, 807, 1423, 1878
TaqI TCGA 5 cut(s) 323, 577, 1110, 1243, 1797
TatI WGTACW 2 cut(s) 17, 1940
TauI GCSGC 3 cut(s) 728, 821, 1664
TfiI GAWTC 4 cut(s) 27, 461, 524, 1656
Tru1I TTAA 3 cut(s) 260, 548, 701
Tru9I TTAA 3 cut(s) 260, 548, 701
TscAI CASTG 6 cut(s) 630, 907, 1024, 1108, 2001, 2040
TseFI GTSAC 4 cut(s) 665, 902, 1549, 2035
TseI GCWGC 7 cut(s) 33, 65, 251, 683, 932, 1592, 1700
Tsp45I GTSAC 4 cut(s) 665, 902, 1549, 2035
TspDTI ATGAA 9 cut(s) 78, 156, 177, 349, 427, 612, 917, 987, 1130
TspGWI ACGGA 2 cut(s) 1161, 1277
TspRI CASTG 6 cut(s) 630, 907, 1024, 1108, 2001, 2040
VneI GTGCAC 1 cut(s) 1422
VpaK11BI GGWCC 6 cut(s) 133, 872, 1584, 1692, 1768, 1915
XapI RAATTY 9 cut(s) 144, 282, 450, 536, 945, 997, 1401, 1443, 1880
XceI RCATGY 4 cut(s) 490, 783, 1649, 1947
XmiI GTMKAC 3 cut(s) 771, 1243, 1851
XmnI GAANNNNTTC 1 cut(s) 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.