Rh2DG110400

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016727
Physical Location & Seq
Reverse (-)
9037528 .. 9039695
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG110400.1

Sequence Viewer

Length: 1308 bp
ATGACTTATAAGGGATTGGCATCTGGAGGAGCTAGAGGAGGTGCACCAATGGCCAGAATTGCAGTATATAAAACTTGTTGGGACACTGGTTGTTATGACGTTGACTTACTAGCAGCTTTTGATGATGCAATTAGAGATGGGGTTCACATTCTATCTCTGTCTCTCGGGCCTGATGCTCCTCAGGGAGACTATTTCACTGATGCCATTTCTGTGGGGTCATTCCATGCTGTTAGGCATGGAATTCTAGTGGTTGCTTCTGCTGGAAATGAAGGAAACCCGGGTTCTGTAACAAATCTCGCCCCATGGATGATTACTGTTGCTGCAAGTTCAACGGATAGGGATTTCACTTCTGATATCGTTCTTGGAAATGGTGTTAATGTCACGGGTGAAAGCCTCAGTGTCTTTGGAATGAAAGCCTCAGCAAGTATCATTTCCGCCTCTGAAGCCTTTGCAGGGTACTTCACTCCATATCAATCCAGTTATTGTTTAGAAAGTTCCTTGAACAAGACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGTCATGCTGAGAGTTCAACAGAGTCGAAGCTTGCAAAAAGTGTAGTAGTTAAAGAAGCTGGTGGTGTCGGGATGGTTCTTATCGATGAGGCAGATATGGATGTTGCCGTCCCATTTGTTATCCCTTCAGCAATCATTAGACGGAGGATAGGAAAACATCTTCTATCTTACATTAATCGCACACGTCTGACAACAAATGAACATACAGCTACTCTTCTGGACAAGAAGCACAAGCCAATTGCAGTGGACCCTGAAGGCAAAAATGGTAATGCATTTGATTACGGCTCAGGCTTCCTGAACCCAACAAGAGCACTTGATCCAGGTCTTATCTTTGACGCACAGCCAGCAGATTATGTATCATTCCTTTGTTCAGTTGGATATGATGAGAAGTCATTGCATCAAATCACACTAGACAACAGTACATGTGCTCAGTCATTCAGAACAGCATCTGACCTTAACTATCCATCTATCACTGTACCAAATCTCGAAGGGAACATCTCAGTAACTAGAACAGTGACCAACGTCGGGAAGCCAAAAAATATCTATAAAGCCGCTGTGTCCTCACCCAAAGGAATCAATGTTACTGTCATCCCAAATCAACTAGTCTTCACCAGCCTCGGTCAGAAGCTAAACTTTACTGTGAATTTCAAGGTGGTTGCTCCATCAAAGGGATATAAATTTGGGTTCTTGTCATGGATGAGTGGAAGATCGAGGGTTACCAGTCCTCTCGTTGTCCAAGTTGCGCATTCAAACTCAGGGCTGATGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

435

Amino Acids

46.34

Weight (kDa)

8.74

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 8 - 146 3.7e-14 Subtilase family
fn3_6 PF17766 330 - 424 3.3e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9
Acc16I TGCGCA 1 cut(s) 1281
AciI CCGC 2 cut(s) 435, 1089
AclWI GGATC 1 cut(s) 848
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 3 cut(s) 240, 1180, 1214
AcuI CTGAAG 3 cut(s) 462, 648, 810
AfaI GTAC 3 cut(s) 458, 958, 1014
AfiI CCNNNNNNNGG 3 cut(s) 453, 1062, 1205
AflIII ACRYGT 2 cut(s) 719, 959
AgsI TTSAA 5 cut(s) 330, 502, 555, 1186, 1287
AhlI ACTAGT 1 cut(s) 1138
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 1 cut(s) 856
AluBI AGCT 6 cut(s) 32, 116, 568, 596, 746, 1165
AluI AGCT 6 cut(s) 32, 116, 568, 596, 746, 1165
Alw21I GWGCWC 3 cut(s) 46, 850, 967
Alw26I GTCTC 2 cut(s) 165, 180
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 1 cut(s) 848
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 2 cut(s) 164, 277
AoxI GGCC 3 cut(s) 51, 167, 513
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 2 cut(s) 113, 320
ApoI RAATTY 3 cut(s) 240, 1180, 1214
AseI ATTAAT 1 cut(s) 711
AspLEI GCGC 1 cut(s) 1282
AspS9I GGNCC 2 cut(s) 167, 784
AsuC2I CCSGG 2 cut(s) 278, 279
AsuHPI GGTGA 3 cut(s) 398, 1092, 1138
AvaI CYCGRG 2 cut(s) 164, 277
AvaII GGWCC 1 cut(s) 784
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 1135
Bbv12I GWGCWC 3 cut(s) 46, 850, 967
BbvCI CCTCAGC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 125, 307
BccI CCATC 4 cut(s) 131, 604, 1009, 1207
BceAI ACGGC 2 cut(s) 629, 835
BciT130I CCWGG 1 cut(s) 858
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 2 cut(s) 165, 180
BcuI ACTAGT 1 cut(s) 1138
BfaI CTAG 6 cut(s) 33, 110, 245, 947, 1044, 1139
BisI GCNGC 3 cut(s) 114, 321, 1089
BlsI GCNGC 3 cut(s) 115, 322, 1090
Bme1390I CCNGG 3 cut(s) 278, 279, 858
Bme18I GGWCC 1 cut(s) 784
BmeT110I CYCGRG 2 cut(s) 164, 277
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 2 cut(s) 167, 784
BmiI GGNNCC 1 cut(s) 786
BmrFI CCNGG 3 cut(s) 278, 279, 858
BmsI GCATC 6 cut(s) 29, 115, 163, 190, 943, 992
BoxI GACNNNNGTC 1 cut(s) 1058
BpiI GAAGAC 1 cut(s) 1135
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 2 cut(s) 418, 823
BpuMI CCSGG 2 cut(s) 278, 279
Bsa29I ATCGAT 1 cut(s) 621
BsaBI GATNNNNATC 1 cut(s) 19
BsaJI CCNNGG 4 cut(s) 277, 302, 510, 1153
Bsc4I CCNNNNNNNGG 3 cut(s) 453, 1062, 1205
Bse1I ACTGG 3 cut(s) 91, 477, 1257
Bse21I CCTNAGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 929
Bse8I GATNNNNATC 1 cut(s) 19
BseBI CCWGG 1 cut(s) 858
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 4 cut(s) 277, 302, 510, 1153
BseGI GGATG 5 cut(s) 312, 615, 643, 1125, 1239
BseJI GATNNNNATC 1 cut(s) 19
BseLI CCNNNNNNNGG 3 cut(s) 453, 1062, 1205
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 8 cut(s) 194, 409, 432, 537, 837, 980, 1050, 1305
BseNI ACTGG 3 cut(s) 91, 477, 1257
BseRI GAGGAG 3 cut(s) 42, 51, 168
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 2 cut(s) 125, 307
BshFI GGCC 3 cut(s) 53, 169, 515
BshVI ATCGAT 1 cut(s) 621
BsiHKAI GWGCWC 3 cut(s) 46, 850, 967
BsiHKCI CYCGRG 2 cut(s) 164, 277
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 95, 632
BslI CCNNNNNNNGG 3 cut(s) 453, 1062, 1205
BsmAI GTCTC 2 cut(s) 165, 180
BsmFI GGGAC 2 cut(s) 95, 632
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 3 cut(s) 53, 169, 515
BsoBI CYCGRG 2 cut(s) 164, 277
Bsp1286I GDGCHC 3 cut(s) 46, 850, 967
Bsp143I GATC 2 cut(s) 853, 1244
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 2 cut(s) 435, 1089
BspANI GGCC 3 cut(s) 53, 169, 515
BspCNI CTCAG 8 cut(s) 193, 408, 431, 538, 836, 979, 1049, 1304
BspDI ATCGAT 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 786
BspPI GGATC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 929
BsrI ACTGG 3 cut(s) 91, 477, 1257
BssECI CCNNGG 4 cut(s) 277, 302, 510, 1153
BssMI GATC 2 cut(s) 853, 1244
BssT1I CCWWGG 2 cut(s) 302, 510
Bst2UI CCWGG 1 cut(s) 858
Bst4CI ACNGT 6 cut(s) 316, 956, 1012, 1051, 1123, 1177
Bst6I CTCTTC 1 cut(s) 756
BstC8I GCNNGC 2 cut(s) 570, 882
BstDEI CTNAG 8 cut(s) 180, 395, 418, 546, 823, 966, 1036, 1291
BstDSI CCRYGG 1 cut(s) 302
BstEII GGTNACC 1 cut(s) 1252
BstF5I GGATG 5 cut(s) 312, 615, 643, 1125, 1239
BstHHI GCGC 1 cut(s) 1282
BstKTI GATC 2 cut(s) 856, 1247
BstMAI GTCTC 2 cut(s) 165, 180
BstMBI GATC 2 cut(s) 853, 1244
BstMWI GCNNNNNNNGC 4 cut(s) 50, 59, 443, 881
BstNI CCWGG 1 cut(s) 858
BstNSI RCATGY 1 cut(s) 963
BstPAI GACNNNNGTC 1 cut(s) 1058
BstPI GGTNACC 1 cut(s) 1252
BstSCI CCNGG 3 cut(s) 276, 277, 856
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 125, 307
BstV2I GAAGAC 1 cut(s) 1135
BstXI CCANNNNNNTGG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 621
Bsu36I CCTNAGG 1 cut(s) 180
BsuRI GGCC 3 cut(s) 53, 169, 515
BsuTUI ATCGAT 1 cut(s) 621
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 5 cut(s) 312, 615, 643, 1125, 1239
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 6 cut(s) 84, 195, 403, 786, 1008, 1056
Cac8I GCNNGC 2 cut(s) 570, 882
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 1 cut(s) 1282
Cfr13I GGNCC 2 cut(s) 167, 784
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 621
CseI GACGC 1 cut(s) 881
Csp6I GTAC 3 cut(s) 457, 957, 1013
CspCI CAANNNNNGTGG 2 cut(s) 762, 797
CviAII CATG 6 cut(s) 224, 236, 303, 542, 960, 1230
CviQI GTAC 3 cut(s) 457, 957, 1013
DdeI CTNAG 8 cut(s) 180, 395, 418, 546, 823, 966, 1036, 1291
DpnI GATC 2 cut(s) 855, 1246
DpnII GATC 2 cut(s) 853, 1244
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
EciI GGCGGA 1 cut(s) 424
Eco130I CCWWGG 2 cut(s) 302, 510
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 3 cut(s) 462, 648, 810
Eco81I CCTNAGG 1 cut(s) 180
Eco88I CYCGRG 2 cut(s) 164, 277
Eco91I GGTNACC 1 cut(s) 1252
EcoO65I GGTNACC 1 cut(s) 1252
EcoRI GAATTC 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 856
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 302, 510
EcoT22I ATGCAT 1 cut(s) 811
ErhI CCWWGG 2 cut(s) 302, 510
FaeI CATG 6 cut(s) 227, 239, 306, 545, 963, 1233
FalI AAGNNNNNCTT 2 cut(s) 1154, 1186
FaqI GGGAC 2 cut(s) 95, 632
FatI CATG 6 cut(s) 223, 235, 302, 541, 959, 1229
Fnu4HI GCNGC 3 cut(s) 114, 321, 1089
FokI GGATG 5 cut(s) 319, 622, 650, 1112, 1246
Fsp4HI GCNGC 3 cut(s) 114, 321, 1089
FspBI CTAG 6 cut(s) 33, 110, 245, 947, 1044, 1139
FspI TGCGCA 1 cut(s) 1281
GlaI GCGC 1 cut(s) 1281
GluI GCNGC 3 cut(s) 114, 321, 1089
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 3 cut(s) 53, 169, 515
HapII CCGG 1 cut(s) 278
HgaI GACGC 1 cut(s) 881
HhaI GCGC 1 cut(s) 1282
Hin1II CATG 6 cut(s) 227, 239, 306, 545, 963, 1233
Hin6I GCGC 1 cut(s) 1280
HinP1I GCGC 1 cut(s) 1280
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 2 cut(s) 560, 1110
HpaII CCGG 1 cut(s) 278
HphI GGTGA 3 cut(s) 398, 1092, 1138
Hpy166II GTNNAC 4 cut(s) 44, 103, 145, 784
Hpy188I TCNGA 6 cut(s) 352, 442, 726, 977, 988, 1161
Hpy188III TCNNGA 6 cut(s) 24, 607, 755, 832, 1022, 1063
Hpy8I GTNNAC 4 cut(s) 44, 103, 145, 784
Hpy99I CGWCG 1 cut(s) 1064
HpyAV CCTTC 5 cut(s) 263, 517, 672, 785, 1019
HpyCH4III ACNGT 6 cut(s) 316, 956, 1012, 1051, 1123, 1177
HpyCH4IV ACGT 3 cut(s) 99, 721, 1059
HpyCH4V TGCA 9 cut(s) 44, 62, 128, 323, 452, 572, 779, 809, 934
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 59, 443, 881
HpyF3I CTNAG 8 cut(s) 180, 395, 418, 546, 823, 966, 1036, 1291
HpySE526I ACGT 3 cut(s) 99, 721, 1059
Hsp92II CATG 6 cut(s) 227, 239, 306, 545, 963, 1233
HspAI GCGC 1 cut(s) 1280
Kzo9I GATC 2 cut(s) 853, 1244
LmnI GCTCC 3 cut(s) 29, 181, 1201
Lsp1109I GCAGC 2 cut(s) 125, 307
LweI GCATC 6 cut(s) 29, 115, 163, 190, 943, 992
MaeI CTAG 6 cut(s) 33, 110, 245, 947, 1044, 1139
MaeII ACGT 3 cut(s) 99, 721, 1059
MaeIII GTNAC 6 cut(s) 286, 379, 1039, 1051, 1117, 1252
MalI GATC 2 cut(s) 855, 1246
MboI GATC 2 cut(s) 853, 1244
MboII GAAGA 4 cut(s) 689, 743, 1135, 1254
MfeI CAATTG 1 cut(s) 774
MhlI GDGCHC 3 cut(s) 46, 850, 967
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 6 cut(s) 57, 129, 240, 774, 1180, 1214
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 892
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 811
MscI TGGCCA 1 cut(s) 53
MseI TTAA 4 cut(s) 375, 588, 711, 993
MslI CAYNNNNRTG 1 cut(s) 209
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 3 cut(s) 278, 279, 858
MunI CAATTG 1 cut(s) 774
Mva1269I GAATGC 1 cut(s) 1282
MvaI CCWGG 1 cut(s) 858
MwoI GCNNNNNNNGC 4 cut(s) 50, 59, 443, 881
NciI CCSGG 2 cut(s) 278, 279
NcoI CCATGG 1 cut(s) 302
NdeII GATC 2 cut(s) 853, 1244
NlaIII CATG 6 cut(s) 227, 239, 306, 545, 963, 1233
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 2 cut(s) 379, 1051
NsbI TGCGCA 1 cut(s) 1281
NsiI ATGCAT 1 cut(s) 811
NspI RCATGY 1 cut(s) 963
PciI ACATGT 1 cut(s) 959
PctI GAATGC 1 cut(s) 1282
PfeI GAWTC 1 cut(s) 1110
PkrI GCNGC 3 cut(s) 115, 322, 1090
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PscI ACATGT 1 cut(s) 959
PshAI GACNNNNGTC 1 cut(s) 1058
PshBI ATTAAT 1 cut(s) 711
PsiI TTATAA 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 856
PspEI GGTNACC 1 cut(s) 1252
PspGI CCWGG 1 cut(s) 856
PspN4I GGNNCC 1 cut(s) 786
PspPI GGNCC 2 cut(s) 167, 784
PstNI CAGNNNCTG 1 cut(s) 986
RsaI GTAC 3 cut(s) 458, 958, 1014
RsaNI GTAC 3 cut(s) 457, 957, 1013
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 4 cut(s) 375, 588, 711, 993
SatI GCNGC 3 cut(s) 114, 321, 1089
Sau3AI GATC 2 cut(s) 853, 1244
Sau96I GGNCC 2 cut(s) 167, 784
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 3 cut(s) 278, 279, 858
SduI GDGCHC 3 cut(s) 46, 850, 967
SfaNI GCATC 6 cut(s) 29, 115, 163, 190, 943, 992
SinI GGWCC 1 cut(s) 784
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 209
SpeI ACTAGT 1 cut(s) 1138
Sse9I AATT 6 cut(s) 57, 129, 240, 774, 1180, 1214
SsiI CCGC 2 cut(s) 435, 1089
SspMI CTAG 6 cut(s) 33, 110, 245, 947, 1044, 1139
StyD4I CCNGG 3 cut(s) 276, 277, 856
StyI CCWWGG 2 cut(s) 302, 510
TaaI ACNGT 6 cut(s) 316, 956, 1012, 1051, 1123, 1177
TaiI ACGT 3 cut(s) 102, 724, 1062
TaqI TCGA 4 cut(s) 563, 621, 1023, 1247
TaqII GACCGA 1 cut(s) 1145
TasI AATT 6 cut(s) 57, 129, 240, 774, 1180, 1214
TatI WGTACW 1 cut(s) 956
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 1 cut(s) 1110
Tru1I TTAA 4 cut(s) 375, 588, 711, 993
Tru9I TTAA 4 cut(s) 375, 588, 711, 993
TscAI CASTG 6 cut(s) 91, 202, 403, 786, 1015, 1056
TseFI GTSAC 2 cut(s) 379, 1051
TseI GCWGC 2 cut(s) 113, 320
Tsp45I GTSAC 2 cut(s) 379, 1051
TspDTI ATGAA 3 cut(s) 282, 425, 750
TspGWI ACGGA 2 cut(s) 347, 694
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 6 cut(s) 91, 202, 403, 786, 1015, 1056
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 784
VspI ATTAAT 1 cut(s) 711
XapI RAATTY 3 cut(s) 240, 1180, 1214
XceI RCATGY 1 cut(s) 963
XmaI CCCGGG 1 cut(s) 277
XspI CTAG 6 cut(s) 33, 110, 245, 947, 1044, 1139
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.