RchiOBHm_Chr2g0096651

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016727
Physical Location & Seq
Reverse (-)
9376913 .. 9380963
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47155

Sequence Viewer

Length: 2292 bp
ATGGCTTCTTTTTGTTCTGCTTGGAGAAGAAGCAGTATTCTTTTCATGTTGCTTTGTGTTCTTGTTGCTCAAATTAGCACTTGTTTTTCATCCAAGGTGTATGTGGTTTATATGGGAAGCAAAAATGGTGAAGACCCAGATGAGATTCTGGCGCAAAACCATCAAATACTTGCTTCTGTTCACACAGGAAGCATAGAGGAAGCTCAAGCATCTCACATTCATAGTTACCGACATGGTTTTAAAGGCTTTGCTGCCAGGTTGACTGATCAGCAAGCTTTCCAAATTTCAAAGATGCCTGGAGTTGTTTCGGTTTTTCCCAACTCGAAAAGGAGTTTGCACACCACTCATTCTTGGGATTTCATGGGTCTTTTGGGTGAACAGACCTTGGAGGTTTCCGGCTTTAACATCAAGAATCAAGTAAATGTCATTGTCGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCAAGTTTTAATGATGACAACATGCCTCCAGTGCCAGCTAGATGGAAAGGGGTTTGTCAATCAGGAGAGGCATTCAATGCTTCAACTTGCAACAGAAAAGTGATTGGGGCTAGATACTATAAGTCCGGTTATGAAGCCGAGGAAGATTCAGCGGACACAGTGGCATTCAGTTCTCCAAGGGACAGTGCCGGTCATGGCAGCCACACAGCCTCTATAGCTGCTGGTCGTTATGTGTCAAACATGACTTATAAGGGATTGGCATCTGGAGGAGCTAGAGGAGGTGCACCAATGGCCAGAATTGCAGTATATAAAACTTGTTGGGACACTGGTTGTTATGACGTTGACTTACTAGCAGCTTTTGATGATGCAATTAGAGATGGGGTTCACATTCTATCTCTGTCTCTCGGGCCTGATGCTCCTCAGGGAGACTATTTCACTGATGCCATTTCTGTGGGGTCATTCCATGCTGTTAGGCATGGAATTCTAGTGGTTGCTTCTGCTGGAAATGAAGGAAACCCGGGTTCTGTAACAAATCTCGCCCCATGGATGATTACTGTTGCTGCAAGTTCAACGGATAGGGATTTCACTTCTGATATCGTTCTTGGAAATGGTGTTAATGTCACGGGTGAAAGCCTCAGTGTCTTTGGAATGAAAGCCTCAGCAAGTATCATTTCCGCCTCTGAAGCCTTTGCAGGGTACTTCACTCCATATCAATCCAGTTATTGTTTAGAAAGTTCCTTGAACAAGACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGTCATGCTGAGAGTTCAACAGAGTCGAAGCTTGCAAAAAGTGTAGTAGTTAAAGAAGCTGGTGGTGTCGGGATGGTTCTTATCGATGAGGCAGATATGGATGTTGCCGTCCCATTTGTTATCCCTTCAGCAATCATTAGACGGAGGATAGGAAAACATCTTCTATCTTACATTAATCGCACACGCCAACCAATGTCAATAATTCTCCCTGCAAAGACTACACTGGGATTGAAACCTGCACCTCATGTTACAGCATTTTCTGCAAAAGGTCCCAATGCTTTGACCCCAGAAATTTTGAAGCCTGATATCACAGCTCCTGGACTGAACATTCTTGCATCTTGGTCTCCAGCAGTAGCTGGTAAGCAGTTCAACATTTTTTCTGGTACATCCATGGCTTGCCCGCATGTAACAGGAATTGCCGCCTTGATAAAAGCCGTTCATCCTTTATGGTCCCCAGCAGCTATTAGATCGGCAATCATGACTACTGCTACTCTTCTGGACAAGAAGCACAAGCCAATTGCAGTGGACCCTGAAGGCAAAAATGGTAATGCATTTGATTACGGCTCAGGCTTCCTGAACCCAACAAGAGCACTTGATCCAGGTCTTATCTTTGACGCACAGCCAGCAGATTATGTATCATTCCTTTGTTCAGTTGGATATGATGAGAAGTCATTGCATCAAATCACACTAGACAACAGTACATGTGCTCAGTCATTCAGAACAGCATCTGACCTTAACTATCCATCTATCACTGTACCAAATCTCGAAGGGAACATCTCAGTAACTAGAACAGTGACCAACGTCGGGAAGCCAAAAAATATCTATAAAGCCGCTGTGTCCTCACCCAAAGGAATCAATGTTACTGTCATCCCAAATCAACTAGTCTTCACCAGCCTCGGTCAGAAGCTAAACTTTACTGTGAATTTCAAGGTGGTTGCTCCATCAAAGGGATATAAATTTGGGTTCTTGTCATGGATGAGTGGAAGATCGAGGGTTACCAGTCCTCTCGTTGTCCAAGTTGCGCATTCAAACTCAGGGCTGATGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

763

Amino Acids

81.51

Weight (kDa)

8.79

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 2.6e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 603 7.1e-47 Subtilase family
fn3_6 PF17766 658 - 752 7.9e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc16I TGCGCA 1 cut(s) 2265
Acc36I ACCTGC 1 cut(s) 1486
AciI CCGC 5 cut(s) 617, 1140, 1643, 1662, 2073
AclWI GGATC 1 cut(s) 1832
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 6 cut(s) 282, 450, 945, 1533, 2164, 2198
AcuI CTGAAG 3 cut(s) 1167, 1353, 1794
AfaI GTAC 4 cut(s) 1163, 1627, 1942, 1998
AfiI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
AflIII ACRYGT 1 cut(s) 1943
AhlI ACTAGT 1 cut(s) 2122
AjnI CCWGG 4 cut(s) 254, 295, 1558, 1840
Alw21I GWGCWC 3 cut(s) 751, 1834, 1951
Alw26I GTCTC 3 cut(s) 870, 885, 1590
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 1832
AlwNI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
Ama87I CYCGRG 2 cut(s) 869, 982
AoxI GGCC 4 cut(s) 455, 756, 872, 1218
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 6 cut(s) 251, 663, 683, 818, 1025, 1700
ApoI RAATTY 6 cut(s) 282, 450, 945, 1533, 2164, 2198
AseI ATTAAT 1 cut(s) 1416
AspLEI GCGC 2 cut(s) 154, 2266
AspS9I GGNCC 4 cut(s) 872, 1511, 1692, 1768
AsuC2I CCSGG 2 cut(s) 983, 984
AsuHPI GGTGA 5 cut(s) 140, 386, 1103, 2076, 2122
AvaI CYCGRG 2 cut(s) 869, 982
AvaII GGWCC 3 cut(s) 1511, 1692, 1768
AxyI CCTNAGG 1 cut(s) 885
BaeGI GKGCMC 1 cut(s) 751
BalI TGGCCA 1 cut(s) 758
BbsI GAAGAC 2 cut(s) 138, 2119
Bbv12I GWGCWC 3 cut(s) 751, 1834, 1951
BbvCI CCTCAGC 1 cut(s) 1123
BbvI GCAGC 6 cut(s) 238, 670, 675, 830, 1012, 1712
BccI CCATC 6 cut(s) 168, 501, 836, 1309, 1993, 2191
BceAI ACGGC 3 cut(s) 1334, 1661, 1819
BciT130I CCWGG 4 cut(s) 256, 297, 1560, 1842
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 2 cut(s) 983, 984
BcoDI GTCTC 3 cut(s) 870, 885, 1590
BcuI ACTAGT 1 cut(s) 2122
BfaI CTAG 8 cut(s) 504, 576, 738, 815, 950, 1931, 2028, 2123
BfmI CTRYAG 1 cut(s) 678
BfuAI ACCTGC 1 cut(s) 1486
BisI GCNGC 8 cut(s) 252, 664, 684, 819, 1026, 1662, 1701, 2073
BlsI GCNGC 8 cut(s) 253, 665, 685, 820, 1027, 1663, 1702, 2074
Bme1390I CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
Bme18I GGWCC 3 cut(s) 1511, 1692, 1768
BmeT110I CYCGRG 2 cut(s) 869, 982
BmgT120I GGNCC 4 cut(s) 872, 1511, 1692, 1768
BmiI GGNNCC 3 cut(s) 1513, 1694, 1770
BmrFI CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
BmrI ACTGGG 1 cut(s) 1475
BmsI GCATC 9 cut(s) 218, 282, 734, 820, 868, 895, 1586, 1927, 1976
BmuI ACTGGG 1 cut(s) 1475
BoxI GACNNNNGTC 1 cut(s) 2042
BpiI GAAGAC 2 cut(s) 138, 2119
BpmI CTGGAG 4 cut(s) 318, 477, 750, 1572
Bpu10I CCTNAGC 2 cut(s) 1123, 1807
BpuEI CTTGAG 1 cut(s) 189
BpuMI CCSGG 2 cut(s) 983, 984
Bsa29I ATCGAT 1 cut(s) 1326
BsaBI GATNNNNATC 1 cut(s) 724
BsaI GGTCTC 1 cut(s) 1590
BsaJI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BsaWI WCCGGW 1 cut(s) 590
Bsc4I CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 6 cut(s) 451, 494, 796, 1182, 1470, 2241
Bse21I CCTNAGG 1 cut(s) 885
Bse3DI GCAATG 1 cut(s) 1913
Bse8I GATNNNNATC 1 cut(s) 724
BseBI CCWGG 4 cut(s) 256, 297, 1560, 1842
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BseGI GGATG 8 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2109, 2223
BseJI GATNNNNATC 1 cut(s) 724
BseLI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
BseMI GCAATG 1 cut(s) 1913
BseMII CTCAG 8 cut(s) 899, 1114, 1137, 1242, 1821, 1964, 2034, 2289
BseNI ACTGG 6 cut(s) 451, 494, 796, 1182, 1470, 2241
BseRI GAGGAG 3 cut(s) 747, 756, 873
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 6 cut(s) 238, 670, 675, 830, 1012, 1712
BseYI CCCAGC 1 cut(s) 1696
BsgI GTGCAG 1 cut(s) 1464
BshFI GGCC 4 cut(s) 457, 758, 874, 1220
BshVI ATCGAT 1 cut(s) 1326
BsiHKAI GWGCWC 3 cut(s) 751, 1834, 1951
BsiHKCI CYCGRG 2 cut(s) 869, 982
BsiSI CCGG 4 cut(s) 396, 591, 654, 983
BslFI GGGAC 5 cut(s) 659, 800, 1337, 1497, 1678
BslI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
BsmAI GTCTC 3 cut(s) 870, 885, 1590
BsmFI GGGAC 5 cut(s) 659, 800, 1337, 1497, 1678
BsmI GAATGC 3 cut(s) 536, 629, 2266
BsnI GGCC 4 cut(s) 457, 758, 874, 1220
Bso31I GGTCTC 1 cut(s) 1590
BsoBI CYCGRG 2 cut(s) 869, 982
Bsp1286I GDGCHC 3 cut(s) 751, 1834, 1951
Bsp143I GATC 4 cut(s) 265, 1709, 1837, 2228
Bsp19I CCATGG 2 cut(s) 1007, 1632
BspACI CCGC 5 cut(s) 617, 1140, 1643, 1662, 2073
BspANI GGCC 4 cut(s) 457, 758, 874, 1220
BspCNI CTCAG 8 cut(s) 898, 1113, 1136, 1243, 1820, 1963, 2033, 2288
BspDI ATCGAT 1 cut(s) 1326
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 3 cut(s) 1513, 1694, 1770
BspMI ACCTGC 1 cut(s) 1486
BspPI GGATC 1 cut(s) 1832
BspTNI GGTCTC 1 cut(s) 1590
BsrDI GCAATG 1 cut(s) 1913
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 6 cut(s) 451, 494, 796, 1182, 1470, 2241
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BssMI GATC 4 cut(s) 265, 1709, 1837, 2228
BssT1I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Bst2UI CCWGG 4 cut(s) 256, 297, 1560, 1842
Bst4CI ACNGT 8 cut(s) 625, 650, 1021, 1940, 1996, 2035, 2107, 2161
Bst6I CTCTTC 1 cut(s) 1740
BstAPI GCANNNNNTGC 2 cut(s) 542, 1502
BstC8I GCNNGC 6 cut(s) 273, 501, 1275, 1639, 1643, 1866
BstDEI CTNAG 8 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2275
BstDSI CCRYGG 2 cut(s) 1007, 1632
BstEII GGTNACC 1 cut(s) 2236
BstF5I GGATG 8 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2109, 2223
BstHHI GCGC 2 cut(s) 154, 2266
BstKTI GATC 4 cut(s) 268, 1712, 1840, 2231
BstMAI GTCTC 3 cut(s) 870, 885, 1590
BstMBI GATC 4 cut(s) 265, 1709, 1837, 2228
BstMWI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
BstNI CCWGG 4 cut(s) 256, 297, 1560, 1842
BstNSI RCATGY 3 cut(s) 490, 1649, 1947
BstPAI GACNNNNGTC 1 cut(s) 2042
BstPI GGTNACC 1 cut(s) 2236
BstSCI CCNGG 6 cut(s) 254, 295, 981, 982, 1558, 1840
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 6 cut(s) 238, 670, 675, 830, 1012, 1712
BstV2I GAAGAC 2 cut(s) 138, 2119
BstXI CCANNNNNNTGG 2 cut(s) 507, 916
Bsu15I ATCGAT 1 cut(s) 1326
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 4 cut(s) 457, 758, 874, 1220
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 2 cut(s) 1007, 1632
BtsCI GGATG 8 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2109, 2223
BtsI GCAGTG 1 cut(s) 1770
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 6 cut(s) 273, 501, 1275, 1639, 1643, 1866
CaiI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 2 cut(s) 154, 2266
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 4 cut(s) 872, 1511, 1692, 1768
Cfr9I CCCGGG 1 cut(s) 982
ClaI ATCGAT 1 cut(s) 1326
CseI GACGC 1 cut(s) 1865
Csp6I GTAC 4 cut(s) 1162, 1626, 1941, 1997
CspCI CAANNNNNGTGG 2 cut(s) 1746, 1781
CviQI GTAC 4 cut(s) 1162, 1626, 1941, 1997
DdeI CTNAG 8 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2275
DpnI GATC 4 cut(s) 267, 1711, 1839, 2230
DpnII GATC 4 cut(s) 265, 1709, 1837, 2228
DraI TTTAAA 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 1740
EarI CTCTTC 1 cut(s) 1740
EciI GGCGGA 1 cut(s) 1129
Eco130I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Eco31I GGTCTC 1 cut(s) 1590
Eco32I GATATC 2 cut(s) 1060, 1549
Eco47I GGWCC 3 cut(s) 1511, 1692, 1768
Eco57I CTGAAG 3 cut(s) 1167, 1353, 1794
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 2 cut(s) 869, 982
Eco91I GGTNACC 1 cut(s) 2236
EcoO109I RGGNCCY 1 cut(s) 1511
EcoO65I GGTNACC 1 cut(s) 2236
EcoRI GAATTC 1 cut(s) 945
EcoRII CCWGG 4 cut(s) 254, 295, 1558, 1840
EcoRV GATATC 2 cut(s) 1060, 1549
EcoT14I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
EcoT22I ATGCAT 1 cut(s) 1795
ErhI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
FalI AAGNNNNNCTT 2 cut(s) 2138, 2170
FaqI GGGAC 5 cut(s) 659, 800, 1337, 1497, 1678
FauI CCCGC 1 cut(s) 1650
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 8 cut(s) 252, 664, 684, 819, 1026, 1662, 1701, 2073
FokI GGATG 8 cut(s) 76, 1024, 1327, 1355, 1615, 1668, 2096, 2230
Fsp4HI GCNGC 8 cut(s) 252, 664, 684, 819, 1026, 1662, 1701, 2073
FspBI CTAG 8 cut(s) 504, 576, 738, 815, 950, 1931, 2028, 2123
FspI TGCGCA 1 cut(s) 2265
GlaI GCGC 2 cut(s) 153, 2265
GluI GCNGC 8 cut(s) 252, 664, 684, 819, 1026, 1662, 1701, 2073
GsaI CCCAGC 1 cut(s) 1700
GsuI CTGGAG 4 cut(s) 318, 477, 750, 1572
HaeIII GGCC 4 cut(s) 457, 758, 874, 1220
HapII CCGG 4 cut(s) 396, 591, 654, 983
HgaI GACGC 1 cut(s) 1865
HhaI GCGC 2 cut(s) 154, 2266
Hin6I GCGC 2 cut(s) 152, 2264
HinP1I GCGC 2 cut(s) 152, 2264
HincII GTYRAC 2 cut(s) 261, 808
HindII GTYRAC 2 cut(s) 261, 808
HindIII AAGCTT 2 cut(s) 273, 1271
HinfI GANTC 6 cut(s) 145, 412, 461, 611, 1265, 2094
HpaII CCGG 4 cut(s) 396, 591, 654, 983
HphI GGTGA 5 cut(s) 140, 386, 1103, 2076, 2122
Hpy166II GTNNAC 7 cut(s) 181, 261, 377, 749, 808, 850, 1768
Hpy188I TCNGA 5 cut(s) 1057, 1147, 1961, 1972, 2145
Hpy188III TCNNGA 9 cut(s) 409, 528, 729, 1312, 1720, 1739, 1816, 2006, 2047
Hpy8I GTNNAC 7 cut(s) 181, 261, 377, 749, 808, 850, 1768
Hpy99I CGWCG 1 cut(s) 2048
HpyAV CCTTC 5 cut(s) 968, 1222, 1377, 1769, 2003
HpyCH4III ACNGT 8 cut(s) 625, 650, 1021, 1940, 1996, 2035, 2107, 2161
HpyCH4IV ACGT 2 cut(s) 804, 2043
HpyF10VI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
HpyF3I CTNAG 8 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2275
HpySE526I ACGT 2 cut(s) 804, 2043
HspAI GCGC 2 cut(s) 152, 2264
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 265, 1709, 1837, 2228
LmnI GCTCC 4 cut(s) 734, 886, 1561, 2185
Lsp1109I GCAGC 6 cut(s) 238, 670, 675, 830, 1012, 1712
LweI GCATC 9 cut(s) 218, 282, 734, 820, 868, 895, 1586, 1927, 1976
MaeI CTAG 8 cut(s) 504, 576, 738, 815, 950, 1931, 2028, 2123
MaeII ACGT 2 cut(s) 804, 2043
MaeIII GTNAC 9 cut(s) 224, 991, 1084, 1489, 1648, 2023, 2035, 2101, 2236
MalI GATC 4 cut(s) 267, 1711, 1839, 2230
MboI GATC 4 cut(s) 265, 1709, 1837, 2228
MboII GAAGA 7 cut(s) 39, 143, 620, 1394, 1727, 2119, 2238
MfeI CAATTG 1 cut(s) 1758
MhlI GDGCHC 3 cut(s) 751, 1834, 1951
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 1 cut(s) 1274
MmeI TCCRAC 1 cut(s) 1876
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 1 cut(s) 1795
MscI TGGCCA 1 cut(s) 758
MseI TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
MslI CAYNNNNRTG 1 cut(s) 914
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 2 cut(s) 617, 2075
MspI CCGG 4 cut(s) 396, 591, 654, 983
MspR9I CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
MunI CAATTG 1 cut(s) 1758
Mva1269I GAATGC 3 cut(s) 536, 629, 2266
MvaI CCWGG 4 cut(s) 256, 297, 1560, 1842
MwoI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
NciI CCSGG 2 cut(s) 983, 984
NcoI CCATGG 2 cut(s) 1007, 1632
NdeII GATC 4 cut(s) 265, 1709, 1837, 2228
NlaIV GGNNCC 3 cut(s) 1513, 1694, 1770
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 1084, 2035
NsbI TGCGCA 1 cut(s) 2265
NsiI ATGCAT 1 cut(s) 1795
NspI RCATGY 3 cut(s) 490, 1649, 1947
PagI TCATGA 1 cut(s) 1719
PciI ACATGT 1 cut(s) 1943
PctI GAATGC 3 cut(s) 536, 629, 2266
PfeI GAWTC 5 cut(s) 145, 412, 461, 611, 2094
PfoI TCCNGGA 1 cut(s) 1558
PkrI GCNGC 8 cut(s) 253, 665, 685, 820, 1027, 1663, 1702, 2074
PleI GAGTC 1 cut(s) 1273
PpsI GAGTC 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 1511
PscI ACATGT 1 cut(s) 1943
PshAI GACNNNNGTC 1 cut(s) 2042
PshBI ATTAAT 1 cut(s) 1416
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1511
Psp6I CCWGG 4 cut(s) 254, 295, 1558, 1840
PspEI GGTNACC 1 cut(s) 2236
PspFI CCCAGC 1 cut(s) 1696
PspGI CCWGG 4 cut(s) 254, 295, 1558, 1840
PspN4I GGNNCC 3 cut(s) 1513, 1694, 1770
PspPI GGNCC 4 cut(s) 872, 1511, 1692, 1768
PspPPI RGGWCCY 1 cut(s) 1511
PstNI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
RsaI GTAC 4 cut(s) 1163, 1627, 1942, 1998
RsaNI GTAC 4 cut(s) 1162, 1626, 1941, 1997
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
SatI GCNGC 8 cut(s) 252, 664, 684, 819, 1026, 1662, 1701, 2073
Sau3AI GATC 4 cut(s) 265, 1709, 1837, 2228
Sau96I GGNCC 4 cut(s) 872, 1511, 1692, 1768
SchI GAGTC 1 cut(s) 1274
ScrFI CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
SduI GDGCHC 3 cut(s) 751, 1834, 1951
SfaNI GCATC 9 cut(s) 218, 282, 734, 820, 868, 895, 1586, 1927, 1976
SfcI CTRYAG 1 cut(s) 678
SinI GGWCC 3 cut(s) 1511, 1692, 1768
SmaI CCCGGG 1 cut(s) 984
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
SpeI ACTAGT 1 cut(s) 2122
SsiI CCGC 5 cut(s) 617, 1140, 1643, 1662, 2073
SspMI CTAG 8 cut(s) 504, 576, 738, 815, 950, 1931, 2028, 2123
StyD4I CCNGG 6 cut(s) 254, 295, 981, 982, 1558, 1840
StyI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
TaaI ACNGT 8 cut(s) 625, 650, 1021, 1940, 1996, 2035, 2107, 2161
TaiI ACGT 2 cut(s) 807, 2046
TaqI TCGA 5 cut(s) 323, 1268, 1326, 2007, 2231
TaqII GACCGA 1 cut(s) 2129
TatI WGTACW 1 cut(s) 1940
TauI GCSGC 2 cut(s) 1664, 2075
TfiI GAWTC 5 cut(s) 145, 412, 461, 611, 2094
Tru1I TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
Tru9I TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
TseFI GTSAC 2 cut(s) 1084, 2035
TseI GCWGC 6 cut(s) 251, 663, 683, 818, 1025, 1700
Tsp45I GTSAC 2 cut(s) 1084, 2035
TspDTI ATGAA 9 cut(s) 34, 78, 209, 349, 427, 612, 987, 1130, 1670
TspGWI ACGGA 2 cut(s) 1052, 1399
TspMI CCCGGG 1 cut(s) 982
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 3 cut(s) 1511, 1692, 1768
VspI ATTAAT 1 cut(s) 1416
XapI RAATTY 6 cut(s) 282, 450, 945, 1533, 2164, 2198
XceI RCATGY 3 cut(s) 490, 1649, 1947
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmaI CCCGGG 1 cut(s) 982
XspI CTAG 8 cut(s) 504, 576, 738, 815, 950, 1931, 2028, 2123
Zsp2I ATGCAT 1 cut(s) 1795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.