Rorug02G0059200

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014297
Physical Location & Seq
Reverse (-)
4559494 .. 4562629
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0059200.1

Sequence Viewer

Length: 1095 bp
ATGGTGAGGATAATACCTATGGCCGCCTCCTCCGTCCGACCCTCCCTCTCCACCTTCAGGTTCGCCGGAGCTTCCCGCCTCGGCGGCCTCTCCCTCACCGCCTTCGCTCCGCCCCGGAGGCAGCTCGCCTTCTTCCACCTCGGTAGCGCTGTGTCGCAGTCGCAGTCGTTTGGTTTGAAAGCTTCGAGGATGTTGAGGAGTGATGGTAAAACCATGGGGGTGGTGTCTGCTGGTAATGCAGCACAGGCAAGCACAACAGCTTCACCGGAGAATGTCCTGGAGTGGGTCAAGCAGGACAAACGGAGGCTTCTCCATGTTGTATACCGCGTTGGGGATTTGGACAGGACCATAAAGTTCTACACGGAGTGCCTGGGAATGAAATTGCTGCGGAAACGTGACATACCAGAGGAAAGATATACAAATGCTTTTCTTGGATATGGGCCGGAGGATTCCCATTTTGTCATCGAACTCACTTACAATTATGGGGTTGACAAGTATGATATTGGAACTGCATTTGGTCATTTTGGTGTTGCTGTTGAGGATGTTGCTAAGACTGTGGAACTTGTAAAAGCTAAGGGAGGCAAGGTAACCCGAGAACCTGGTCCAGTGAAAGGTGGAAATACAGTAATTGCTTTTGTTGAAGATCCTGATGGTTATAAGTTTGAACTCTTGGAAAGAGGGCCTACACCTGAGCCCTTGTGTCAAATTATGCTTCGTGTAGGTGATCTTGATCGTTCCATAGCTTTTTATGAGAAGGCTTTTGGCATGGAGCTTCTTCGCAAGAGAGATAATCCAGAGTACAAGTATACTATCGCAATGCTTGGTTATGGCCCTGAAGACAAAAATGCTGTTCTGGAATTGACATACAACTATGGGGTCACTGAATACGACAAAGGAAATGCTTATGCTCAGATAGCGGTAGGCACGGATGATGTCTATAAAACTGCTGAAGCAATTAAACTCTGCGGAGGCAAGATTACCCGGGAACCTGGGCCATTACCTGGTCTTAACACAAAGATCACTGCTTGCTTGGACCCTGATGGTTGGAAGTCGGTTTTCGTGGATAATGTTGATTTCCTCAAGGAATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

364

Amino Acids

40.17

Weight (kDa)

7.6

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 102 - 223 4.9e-27 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 105 - 212 2e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 235 - 350 5.5e-19 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51500
fragaria_vesca FvH4_1g09810 FvH4_1g09810
malus_domestica MD02G1107400.v1.1 MD15G1226600.v1.1
prunus_persica Prupe.7G188600_v2.0.a1
pyrus_communis pycom15g20150
rosa_chinensis RchiOBHm_Chr2g0096641
rosa_laevigata RLG00000016657
rosa_multiflora Rmu_sc0003565.1_g000016
rosa_roxburghii Rroxscaffold_2G00145460
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2AG106600 Rh2BG109000 Rh2CG110800 Rh2DG110300
rosa_wichuraiana Rw2G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccI GTMKAC 2 cut(s) 321, 806
AccII CGCG 1 cut(s) 327
AciI CCGC 9 cut(s) 24, 76, 84, 99, 110, 325, 388, 917, 966
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 3 cut(s) 40, 855, 969
AdeI CACNNNGTG 1 cut(s) 366
AfaI GTAC 1 cut(s) 800
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 5 cut(s) 57, 283, 331, 611, 1001
AgsI TTSAA 3 cut(s) 178, 641, 665
AjnI CCWGG 5 cut(s) 276, 369, 598, 988, 1000
AluBI AGCT 7 cut(s) 71, 124, 182, 260, 572, 743, 772
AluI AGCT 7 cut(s) 71, 124, 182, 260, 572, 743, 772
AlwI GGATC 1 cut(s) 638
Ama87I CYCGRG 2 cut(s) 591, 981
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 6 cut(s) 21, 85, 440, 680, 829, 992
ApeKI GCWGC 3 cut(s) 121, 239, 385
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 7 cut(s) 345, 440, 602, 680, 830, 992, 1033
AsuC2I CCSGG 3 cut(s) 115, 982, 983
AsuHPI GGTGA 4 cut(s) 16, 88, 255, 734
AvaI CYCGRG 2 cut(s) 591, 981
AvaII GGWCC 3 cut(s) 345, 602, 1033
BanII GRGCYC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 133, 251, 372
BccI CCATC 3 cut(s) 197, 644, 1034
BciT130I CCWGG 5 cut(s) 278, 371, 600, 990, 1002
BcnI CCSGG 3 cut(s) 115, 982, 983
BfoI RGCGCY 1 cut(s) 150
BglI GCCNNNNNGGC 2 cut(s) 84, 118
BisI GCNGC 5 cut(s) 24, 85, 122, 240, 386
BlsI GCNGC 5 cut(s) 25, 86, 123, 241, 387
Bme1390I CCNGG 8 cut(s) 115, 278, 371, 600, 982, 983, 990, 1002
Bme18I GGWCC 3 cut(s) 345, 602, 1033
BmeT110I CYCGRG 2 cut(s) 591, 981
BmgT120I GGNCC 7 cut(s) 345, 440, 602, 680, 830, 992, 1033
BmiI GGNNCC 2 cut(s) 987, 1035
BmrFI CCNGG 8 cut(s) 115, 278, 371, 600, 982, 983, 990, 1002
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 2 cut(s) 573, 690
BpuEI CTTGAG 1 cut(s) 1064
BpuMI CCSGG 3 cut(s) 115, 982, 983
BsaBI GATNNNNATC 1 cut(s) 729
BsaJI CCNNGG 7 cut(s) 79, 113, 139, 213, 370, 981, 989
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 190, 220, 570, 600
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 283, 331, 611, 1001
Bse1I ACTGG 1 cut(s) 605
Bse3DI GCAATG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 5 cut(s) 278, 371, 600, 990, 1002
BseDI CCNNGG 7 cut(s) 79, 113, 139, 213, 370, 981, 989
BseGI GGATG 3 cut(s) 195, 547, 934
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 5 cut(s) 57, 283, 331, 611, 1001
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 2 cut(s) 681, 923
BseNI ACTGG 1 cut(s) 605
BseRI GAGGAG 2 cut(s) 19, 211
BseXI GCAGC 3 cut(s) 133, 251, 372
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 6 cut(s) 23, 87, 442, 682, 831, 994
BsiHKCI CYCGRG 2 cut(s) 591, 981
BsiSI CCGG 5 cut(s) 66, 115, 266, 443, 982
BslI CCNNNNNNNGG 5 cut(s) 57, 283, 331, 611, 1001
BsnI GGCC 6 cut(s) 23, 87, 442, 682, 831, 994
BsoBI CYCGRG 2 cut(s) 591, 981
Bsp1286I GDGCHC 1 cut(s) 696
Bsp143I GATC 4 cut(s) 643, 724, 730, 1017
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 9 cut(s) 24, 76, 84, 99, 110, 325, 388, 917, 966
BspANI GGCC 6 cut(s) 23, 87, 442, 682, 831, 994
BspCNI CTCAG 2 cut(s) 682, 922
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 2 cut(s) 987, 1035
BspPI GGATC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 822
BsrI ACTGG 1 cut(s) 605
BssECI CCNNGG 7 cut(s) 79, 113, 139, 213, 370, 981, 989
BssMI GATC 4 cut(s) 643, 724, 730, 1017
BssNAI GTATAC 2 cut(s) 322, 807
BssT1I CCWWGG 1 cut(s) 213
Bst1107I GTATAC 2 cut(s) 322, 807
Bst2UI CCWGG 5 cut(s) 278, 371, 600, 990, 1002
Bst4CI ACNGT 2 cut(s) 556, 625
BstC8I GCNNGC 3 cut(s) 126, 250, 1027
BstDEI CTNAG 4 cut(s) 549, 573, 690, 909
BstDSI CCRYGG 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 586
BstF5I GGATG 3 cut(s) 195, 547, 934
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 4 cut(s) 646, 727, 733, 1020
BstMBI GATC 4 cut(s) 643, 724, 730, 1017
BstMWI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 914
BstNI CCWGG 5 cut(s) 278, 371, 600, 990, 1002
BstPI GGTNACC 1 cut(s) 586
BstSCI CCNGG 8 cut(s) 113, 276, 369, 598, 980, 981, 988, 1000
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 3 cut(s) 133, 251, 372
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 1 cut(s) 643
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 1 cut(s) 643
BstZ17I GTATAC 2 cut(s) 322, 807
BsuRI GGCC 6 cut(s) 23, 87, 442, 682, 831, 994
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 3 cut(s) 195, 547, 934
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 3 cut(s) 612, 879, 1020
Cac8I GCNNGC 3 cut(s) 126, 250, 1027
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 7 cut(s) 345, 440, 602, 680, 830, 992, 1033
Cfr9I CCCGGG 1 cut(s) 981
CsiI ACCWGGT 2 cut(s) 598, 1000
Csp6I GTAC 1 cut(s) 799
CviAII CATG 3 cut(s) 214, 314, 766
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 4 cut(s) 549, 573, 690, 909
DpnI GATC 4 cut(s) 645, 726, 732, 1019
DpnII GATC 4 cut(s) 643, 724, 730, 1017
DraIII CACNNNGTG 1 cut(s) 366
EaeI YGGCCR 1 cut(s) 21
EciI GGCGGA 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 696
Eco47I GGWCC 3 cut(s) 345, 602, 1033
Eco47III AGCGCT 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 40, 855, 969
Eco88I CYCGRG 2 cut(s) 591, 981
Eco91I GGTNACC 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 680
EcoO65I GGTNACC 1 cut(s) 586
EcoRII CCWGG 5 cut(s) 276, 369, 598, 988, 1000
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 3 cut(s) 217, 317, 769
FatI CATG 3 cut(s) 213, 313, 765
FauI CCCGC 1 cut(s) 83
FblI GTMKAC 2 cut(s) 321, 806
Fnu4HI GCNGC 5 cut(s) 24, 85, 122, 240, 386
FokI GGATG 3 cut(s) 202, 554, 941
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 5 cut(s) 24, 85, 122, 240, 386
GlaI GCGC 1 cut(s) 148
GluI GCNGC 5 cut(s) 24, 85, 122, 240, 386
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 6 cut(s) 23, 87, 442, 682, 831, 994
HapII CCGG 5 cut(s) 66, 115, 266, 443, 982
HhaI GCGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 217, 317, 769
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 1 cut(s) 449
HpaII CCGG 5 cut(s) 66, 115, 266, 443, 982
HphI GGTGA 4 cut(s) 16, 88, 255, 734
Hpy166II GTNNAC 3 cut(s) 322, 490, 807
Hpy188I TCNGA 2 cut(s) 38, 912
Hpy188III TCNNGA 4 cut(s) 647, 728, 794, 854
Hpy8I GTNNAC 3 cut(s) 322, 490, 807
HpyAV CCTTC 4 cut(s) 64, 112, 139, 748
HpyCH4III ACNGT 2 cut(s) 556, 625
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 2 cut(s) 239, 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 914
HpyF3I CTNAG 4 cut(s) 549, 573, 690, 909
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 3 cut(s) 217, 317, 769
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 4 cut(s) 643, 724, 730, 1017
LmnI GCTCC 3 cut(s) 68, 112, 769
Lsp1109I GCAGC 3 cut(s) 133, 251, 372
MabI ACCWGGT 2 cut(s) 598, 1000
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 3 cut(s) 395, 586, 877
MalI GATC 4 cut(s) 645, 726, 732, 1019
MboI GATC 4 cut(s) 643, 724, 730, 1017
MboII GAAGA 4 cut(s) 124, 653, 767, 848
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 7 cut(s) 380, 478, 627, 705, 857, 954, 1085
MmeI TCCRAC 2 cut(s) 61, 1025
MseI TTAA 2 cut(s) 957, 1008
MslI CAYNNNNRTG 2 cut(s) 218, 525
MspI CCGG 5 cut(s) 66, 115, 266, 443, 982
MspR9I CCNGG 8 cut(s) 115, 278, 371, 600, 982, 983, 990, 1002
MvaI CCWGG 5 cut(s) 278, 371, 600, 990, 1002
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 914
NciI CCSGG 3 cut(s) 115, 982, 983
NcoI CCATGG 1 cut(s) 213
NdeII GATC 4 cut(s) 643, 724, 730, 1017
NlaIII CATG 3 cut(s) 217, 317, 769
NlaIV GGNNCC 2 cut(s) 987, 1035
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 395, 877
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 1001
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 5 cut(s) 25, 86, 123, 241, 387
PsiI TTATAA 1 cut(s) 657
Psp6I CCWGG 5 cut(s) 276, 369, 598, 988, 1000
PspEI GGTNACC 1 cut(s) 586
PspGI CCWGG 5 cut(s) 276, 369, 598, 988, 1000
PspN4I GGNNCC 2 cut(s) 987, 1035
PspPI GGNCC 7 cut(s) 345, 440, 602, 680, 830, 992, 1033
PsuI RGATCY 1 cut(s) 643
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 2 cut(s) 218, 525
SaqAI TTAA 2 cut(s) 957, 1008
SatI GCNGC 5 cut(s) 24, 85, 122, 240, 386
Sau3AI GATC 4 cut(s) 643, 724, 730, 1017
Sau96I GGNCC 7 cut(s) 345, 440, 602, 680, 830, 992, 1033
ScrFI CCNGG 8 cut(s) 115, 278, 371, 600, 982, 983, 990, 1002
SduI GDGCHC 1 cut(s) 696
SexAI ACCWGGT 2 cut(s) 598, 1000
SinI GGWCC 3 cut(s) 345, 602, 1033
SmaI CCCGGG 1 cut(s) 983
SmiMI CAYNNNNRTG 2 cut(s) 218, 525
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
Sse9I AATT 7 cut(s) 380, 478, 627, 705, 857, 954, 1085
SsiI CCGC 9 cut(s) 24, 76, 84, 99, 110, 325, 388, 917, 966
StyD4I CCNGG 8 cut(s) 113, 276, 369, 598, 980, 981, 988, 1000
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 556, 625
TaiI ACGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 185, 465
TasI AATT 7 cut(s) 380, 478, 627, 705, 857, 954, 1085
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 26, 87
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 957, 1008
Tru9I TTAA 2 cut(s) 957, 1008
TscAI CASTG 3 cut(s) 612, 886, 1027
TseFI GTSAC 2 cut(s) 395, 877
TseI GCWGC 3 cut(s) 121, 239, 385
Tsp45I GTSAC 2 cut(s) 395, 877
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 4 cut(s) 22, 316, 377, 941
TspMI CCCGGG 1 cut(s) 981
TspRI CASTG 3 cut(s) 612, 886, 1027
Van91I CCANNNNNTGG 1 cut(s) 1001
VpaK11BI GGWCC 3 cut(s) 345, 602, 1033
XmaI CCCGGG 1 cut(s) 981
XmiI GTMKAC 2 cut(s) 321, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.