RLG00000016657

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014297
Physical Location & Seq
Forward (+)
8626352 .. 8628859
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016657

Sequence Viewer

Length: 564 bp
ATGATTAATGCTTATCAGGTTATTAAGATTAACACAGATGAGAAGATAGTAGAGCTGTTTCGAAGCTCTGCCCAAAAGCCCCAATCTTCAGCGGTGCCGCTTCAGCATCCGGTGAAAATGCAGGATTCAAAGATCCCCCTTTCGGATGAATTTTCGATTCCCAAGTCACGGAAGAAGAAAGTCTCGGCCCAGAAAGGCCCCCCCTTGTTTCAGGTACAAGGGAACGATGTGAATGGGGCTCCTTCTCTTCCCCTAAAAGCTGGGAACAAGCCATCCAAAAGGAACTTGAAGAGGGAAGTTTCTCCAATGGTCCAGCAACCAGAGAGGTCAAACTCAAATTCGCTGCCTGATTCATCTTCGTCTGGAAGCAACTACCGTGAGCTAAGGCTTAAGTACTTGATGATGGAGGAAGAGAGCTTTGCAGTGGGGAAAGATCTACGAGAGGTTGAAGACGAGGTTAAGACCCTTGAGGAGGAGAAGTTTGCACTCTTGGATGAGCTTGTTGTGTTAGAAGGCCTCGTTGATCCTTCAGAGTTGCACCCCCAGGGGTTGCATTCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.87

Weight (kDa)

6.34

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51500
fragaria_vesca FvH4_1g09810 FvH4_1g09810
malus_domestica MD02G1107400.v1.1 MD15G1226600.v1.1
prunus_persica Prupe.7G188600_v2.0.a1
pyrus_communis pycom15g20150
rosa_chinensis RchiOBHm_Chr2g0096641
rosa_laevigata RLG00000016657
rosa_multiflora Rmu_sc0003565.1_g000016
rosa_roxburghii Rroxscaffold_2G00145460
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2AG106600 Rh2BG109000 Rh2CG110800 Rh2DG110300
rosa_wichuraiana Rw2G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AciI CCGC 2 cut(s) 92, 98
AclWI GGATC 2 cut(s) 127, 518
AcsI RAATTY 2 cut(s) 149, 337
AcuI CTGAAG 3 cut(s) 72, 86, 513
AfaI GTAC 2 cut(s) 216, 395
AfiI CCNNNNNNNGG 3 cut(s) 142, 168, 472
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 3 cut(s) 129, 289, 449
AjnI CCWGG 1 cut(s) 543
AloI GAACNNNNNNTCC 2 cut(s) 257, 289
AluBI AGCT 6 cut(s) 55, 66, 260, 382, 417, 499
AluI AGCT 6 cut(s) 55, 66, 260, 382, 417, 499
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 2 cut(s) 127, 518
AoxI GGCC 3 cut(s) 186, 196, 514
ApeKI GCWGC 1 cut(s) 343
ApoI RAATTY 2 cut(s) 149, 337
AseI ATTAAT 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 187, 197, 310
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 330
BccI CCATC 2 cut(s) 280, 397
BciT130I CCWGG 1 cut(s) 545
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 1 cut(s) 562
BfrI CTTAAG 1 cut(s) 389
BglII AGATCT 1 cut(s) 433
BisI GCNGC 2 cut(s) 98, 344
BlsI GCNGC 2 cut(s) 99, 345
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 545
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 3 cut(s) 187, 197, 310
BmiI GGNNCC 3 cut(s) 96, 199, 240
BmrFI CCNGG 1 cut(s) 545
BmsI GCATC 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 383
Bpu14I TTCGAA 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 543, 544
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 168, 472
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 543, 544
BseGI GGATG 5 cut(s) 106, 151, 272, 499, 557
BseLI CCNNNNNNNGG 3 cut(s) 142, 168, 472
BseRI GAGGAG 2 cut(s) 485, 488
BseXI GCAGC 1 cut(s) 330
BseYI CCCAGC 1 cut(s) 260
BshFI GGCC 3 cut(s) 188, 198, 516
BshNI GGYRCC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 3 cut(s) 142, 168, 472
BsmAI GTCTC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 3 cut(s) 188, 198, 516
Bsp119I TTCGAA 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 3 cut(s) 132, 433, 523
BspACI CCGC 2 cut(s) 92, 98
BspANI GGCC 3 cut(s) 188, 198, 516
BspLI GGNNCC 3 cut(s) 96, 199, 240
BspPI GGATC 2 cut(s) 127, 518
BspT104I TTCGAA 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 543, 544
BssMI GATC 3 cut(s) 132, 433, 523
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 3 cut(s) 252, 284, 405
BstAFI CTTAAG 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 383
BstENI CCTNNNNNAGG 1 cut(s) 470
BstF5I GGATG 5 cut(s) 106, 151, 272, 499, 557
BstKTI GATC 3 cut(s) 135, 436, 526
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 3 cut(s) 132, 433, 523
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 330
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 2 cut(s) 132, 433
BstYI RGATCY 2 cut(s) 132, 433
BsuRI GGCC 3 cut(s) 188, 198, 516
BtsCI GGATG 5 cut(s) 106, 151, 272, 499, 557
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 429
Cfr13I GGNCC 3 cut(s) 187, 197, 310
Csp6I GTAC 2 cut(s) 215, 394
CviQI GTAC 2 cut(s) 215, 394
DdeI CTNAG 1 cut(s) 383
DpnI GATC 3 cut(s) 134, 435, 525
DpnII GATC 3 cut(s) 132, 433, 523
Eam1104I CTCTTC 3 cut(s) 252, 284, 405
EarI CTCTTC 3 cut(s) 252, 284, 405
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 3 cut(s) 72, 86, 513
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 98, 344
FokI GGATG 5 cut(s) 93, 158, 259, 506, 544
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 2 cut(s) 98, 344
FspBI CTAG 1 cut(s) 562
GluI GCNGC 2 cut(s) 98, 344
GsaI CCCAGC 1 cut(s) 264
HaeIII GGCC 3 cut(s) 188, 198, 516
HapII CCGG 1 cut(s) 110
HinfI GANTC 3 cut(s) 125, 157, 350
HpaII CCGG 1 cut(s) 110
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 145, 532
Hpy188III TCNNGA 1 cut(s) 363
HpyAV CCTTC 3 cut(s) 252, 506, 537
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4V TGCA 5 cut(s) 121, 422, 485, 538, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
HpyF3I CTNAG 1 cut(s) 383
Kzo9I GATC 3 cut(s) 132, 433, 523
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 1 cut(s) 330
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 165
MalI GATC 3 cut(s) 134, 435, 525
MboI GATC 3 cut(s) 132, 433, 523
MboII GAAGA 9 cut(s) 55, 78, 184, 187, 239, 301, 348, 422, 461
MflI RGATCY 2 cut(s) 132, 433
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 2 cut(s) 149, 337
MnlI CCTC 8 cut(s) 285, 318, 400, 436, 448, 463, 466, 527
MseI TTAA 5 cut(s) 6, 24, 30, 390, 459
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 545
Mva1269I GAATGC 1 cut(s) 553
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 103
NdeII GATC 3 cut(s) 132, 433, 523
NlaIV GGNNCC 3 cut(s) 96, 199, 240
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 165
NspV TTCGAA 1 cut(s) 61
PasI CCCWGGG 1 cut(s) 544
PceI AGGCCT 1 cut(s) 516
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 3 cut(s) 125, 157, 350
PkrI GCNGC 2 cut(s) 99, 345
PshBI ATTAAT 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 3 cut(s) 96, 199, 240
PspPI GGNCC 3 cut(s) 187, 197, 310
PsuI RGATCY 2 cut(s) 132, 433
RsaI GTAC 2 cut(s) 216, 395
RsaNI GTAC 2 cut(s) 215, 394
SaqAI TTAA 5 cut(s) 6, 24, 30, 390, 459
SatI GCNGC 2 cut(s) 98, 344
Sau3AI GATC 3 cut(s) 132, 433, 523
Sau96I GGNCC 3 cut(s) 187, 197, 310
ScaI AGTACT 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 545
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 61
SinI GGWCC 1 cut(s) 310
SmlI CTYRAG 2 cut(s) 389, 467
SmoI CTYRAG 2 cut(s) 389, 467
Sse9I AATT 2 cut(s) 149, 337
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 2 cut(s) 92, 98
SspMI CTAG 1 cut(s) 562
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 377
TaqI TCGA 2 cut(s) 61, 155
TasI AATT 2 cut(s) 149, 337
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 3 cut(s) 125, 157, 350
Tru1I TTAA 5 cut(s) 6, 24, 30, 390, 459
Tru9I TTAA 5 cut(s) 6, 24, 30, 390, 459
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 165
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 3 cut(s) 162, 342, 546
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 1 cut(s) 429
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 310
VspI ATTAAT 1 cut(s) 6
XagI CCTNNNNNAGG 1 cut(s) 470
XapI RAATTY 2 cut(s) 149, 337
XspI CTAG 1 cut(s) 562
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.