pycom02g08610

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016727
Physical Location & Seq
Reverse (-)
5958722 .. 5962269
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08610.3

Sequence Viewer

Length: 2295 bp
ATGGGGTCTTTTTGTTGCACTGGGAGAATCAGCAGCGTTCTGCTTCTGTTCTTATATGTTCTTGCTGCTGAAATTAGCATTTGCCTTTCATCCAAGGTGTATGTAGTTTATATGGGAAGCAAAAATGGTGAGGACCCCGATGAAATTTTGACACAAAACCATGAAATGCTTGCTTTTGTGCATACTGGAAGCATGGAGGAAGCTCGGGCATCTCACCTTTACAGTTACAGACATGGCTTCAGAGGTTTTGCAGCCAAGTTGACTGACCTCCAAGCTTTCCAAATTTCCAAGATGCCGGGAGTTGTTTCGGTTTTCCCCAATTCGAAAAGAAGCTTGCACACCACACATTCCTGGGATTTCATGGGCCTTTTGGGTGAAGAAACCATGGAGATTATTGGGTATTCCACCAAGAATCAAGTTGATGTCATTGTTGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCAAGTTTTGATGATGCCAACATGCCTCCTGTGCCAGCTAGATGGAAGGGGCGTTGTGAATCAGGAGAAAAATTCACCGCTTTAACTTGCAACAGGAAAGTGATTGGTGCTCGATACTATAATTCTGGTTATGAAGCCGAGGAAGAGTCAACAACTACAGTGTCATTCAGGTCACCAAGGGACAGCTCAGGTCATGGCAGTCACACAGCCTCTATAGCTGCTGGTCGGTACGTGTTAAACATGACTTATAAGGGATTGGCAGCTGGAGGGGCAAGAGGAGGGGCACCAATGGCTAGGATTGCAGTATACAAGACATGTTGGAACTCTGGTTGTTATGACGTTGACTTACTAGCGGCATTTGATGATGCTATCAGAGATGGGGTTGACATCATGTCTGTGTCTCTAGGTCCTGATGCTCCTCAGGGAGACTATTTCAGTGACGCCATTTCTATCGGATCATTTCATGCTGCTAGGCATGGAATTCTAGTGGTTGCTTCTTCTGGAAATGAAGGAAACCCGGGTTCTGCAACTAATTTAGCCCCATGGATGCTCACTGTTGGTGCAAGTTCAACGGATAGGGATTTCACTTCTGATATCATACTAGAAAATGGTGCTAATTTCACGGGTGAAAGTCTCAGTCTCTTCGAAATGAAAGCCTCAGCAAGGATTATTTCTGCCTCTGAAGCCAATGCAGGGTATTTTACTCCGTATCAATCCAGTTACTGTTTAGAAAGTTCCTTGAATAGAACCAAGGCCAGAGGGAAGGTTCTGGTATGTCGACACGCTGAGAGTTCAACGGAGTCAAAGCTTGCTAAAAGTATGCTAGTGGAAAAGGCTGGTGGTGTCGGGATGGTTCTCATTGATGATGCAGATAAGGATATTGCTGTCCCATTTGTTATCCCTTCTGCTATCGTTGGACAGAGGATAGGGCATCAAATTTTATCTTACATTATACGTGCACGAAAACCCAAGTCACGAATTTTGCCTGCAAAAACTATACTGGGACTGAAACCTGCACCTCGTGTTACAGCATTTTCTTCAAAAGGCCCCAATTCTTTGACACCGGAGATTTTGAAGCCCGATGTCACAGCTCCTGGACTAAATATACTAGCATCTTGGTCCCCAGCAGCAGGGAATAAGCTGTTCAACATTCTTTCTGGTACTTCCATGGCTTGCCCACATGTAACAGGAATCGCCGCCTTGATCAAAGCCGTTTATCCATCATGGTCCCCAGCAGCCATTAGATCTGCAATCATGACTACAGCTACACTTCTGGATAAGAACTACAAACGCATCCTAGTGGACCCTGAAGGAAGAAGGGGCAACGCATTCGATTATGGCTCAGGCTTTGTGAACCCGAAAAGAGTCCTTGACCCAGGTCTTGTCTACGACGCACATTCAGCAGACTACGTAGAATTCCTTTGTTCAGTTGGTTATGACAAGAAGGCAGTCCACCAAATCACACGAGACAATAGTACATGTAAGCAGGCATTCCGAACAGCATCTGACCTGAACTATCCATCTATCACAGTGCCATATCTTGTAGGCAACTTCTCGGTAACTAGAACAGTGACCAATGTTGGGAAGCCAAAGAGTATCTATAGAGCAGCGGTGTCCCCACCCATCGGAATCAATGTTACCATTGTCCCAAATCGATTAATCTTCAATAGGTTCGGCCAGAAGATAAAGTTCACAGTAAACTTCAAGGTGGCTGCTCCACCAGCGAAGGGATATGCATTCGGGTTCTTTTCGTGGGTACGTGGACGATCAAGAGTTACCAGCCCCCTGGTTGTCCGAGTTGCACATTCAAACTCAGGGCTGCTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

765

Amino Acids

82.47

Weight (kDa)

9.18

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 1486
AccB1I GGYRCC 1 cut(s) 748
AccI GTMKAC 3 cut(s) 771, 1243, 1851
AciI CCGC 4 cut(s) 543, 818, 1662, 2075
AclWI GGATC 1 cut(s) 928
AcoI YGGCCR 1 cut(s) 2140
AcsI RAATTY 8 cut(s) 144, 282, 450, 536, 945, 1401, 1443, 1880
AcuI CTGAAG 3 cut(s) 223, 1167, 1794
AcyI GRCGYC 1 cut(s) 906
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 4 cut(s) 695, 1627, 1942, 2223
AfiI CCNNNNNNNGG 6 cut(s) 1128, 1158, 1595, 2046, 2090, 2192
AflIII ACRYGT 4 cut(s) 696, 779, 1645, 1943
AgsI TTSAA 9 cut(s) 1035, 1207, 1260, 1506, 1540, 1612, 2131, 2170, 2274
AhdI GACNNNNNGTC 1 cut(s) 856
AjnI CCWGG 4 cut(s) 350, 1558, 1840, 2250
Alw21I GWGCWC 2 cut(s) 577, 1426
Alw26I GTCTC 5 cut(s) 870, 885, 1103, 1109, 1926
Alw44I GTGCAC 1 cut(s) 1422
AlwI GGATC 1 cut(s) 928
AlwNI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
Ama87I CYCGRG 2 cut(s) 204, 982
AoxI GGCC 5 cut(s) 364, 455, 1218, 1510, 2140
ApaLI GTGCAC 1 cut(s) 1422
ApoI RAATTY 8 cut(s) 144, 282, 450, 536, 945, 1401, 1443, 1880
AseI ATTAAT 1 cut(s) 2123
AspS9I GGNCC 7 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768
AsuC2I CCSGG 3 cut(s) 297, 983, 984
AsuHPI GGTGA 6 cut(s) 140, 206, 386, 532, 630, 1103
AsuII TTCGAA 2 cut(s) 323, 1110
AvaI CYCGRG 2 cut(s) 204, 982
AvaII GGWCC 5 cut(s) 133, 872, 1584, 1692, 1768
AxyI CCTNAGG 1 cut(s) 885
BaeGI GKGCMC 2 cut(s) 751, 1426
BanI GGYRCC 1 cut(s) 748
BauI CACGAG 2 cut(s) 1485, 1929
Bbv12I GWGCWC 2 cut(s) 577, 1426
BbvCI CCTCAGC 2 cut(s) 1123, 2288
BccI CCATC 6 cut(s) 501, 836, 1309, 1693, 1993, 2096
BceAI ACGGC 1 cut(s) 1661
BcgI CGANNNNNNTGC 2 cut(s) 1777, 1811
BciT130I CCWGG 4 cut(s) 352, 1560, 1842, 2252
BclI TGATCA 1 cut(s) 1668
BcnI CCSGG 3 cut(s) 297, 983, 984
BcoDI GTCTC 5 cut(s) 870, 885, 1103, 1109, 1926
BfmI CTRYAG 4 cut(s) 621, 678, 1725, 2065
BfuAI ACCTGC 1 cut(s) 1486
BglII AGATCT 1 cut(s) 1709
Bme1390I CCNGG 7 cut(s) 297, 352, 983, 984, 1560, 1842, 2252
Bme18I GGWCC 5 cut(s) 133, 872, 1584, 1692, 1768
BmeRI GACNNNNNGTC 1 cut(s) 856
BmeT110I CYCGRG 2 cut(s) 204, 982
BmgT120I GGNCC 7 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768
BmiI GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
BmrFI CCNGG 7 cut(s) 297, 352, 983, 984, 1560, 1842, 2252
BmrI ACTGGG 2 cut(s) 30, 1475
BmuI ACTGGG 2 cut(s) 30, 1475
BoxI GACNNNNGTC 1 cut(s) 1842
BpmI CTGGAG 1 cut(s) 750
Bpu10I CCTNAGC 4 cut(s) 652, 1123, 1807, 2288
Bpu14I TTCGAA 2 cut(s) 323, 1110
BpuMI CCSGG 3 cut(s) 297, 983, 984
Bsa29I ATCGAT 1 cut(s) 2119
BsaAI YACGTR 4 cut(s) 697, 1421, 1876, 2225
BsaHI GRCGYC 1 cut(s) 906
BsaWI WCCGGW 1 cut(s) 1528
Bsc4I CCNNNNNNNGG 6 cut(s) 1128, 1158, 1595, 2046, 2090, 2192
Bse1I ACTGG 5 cut(s) 25, 190, 451, 1182, 1470
Bse21I CCTNAGG 1 cut(s) 885
BseBI CCWGG 4 cut(s) 352, 1560, 1842, 2252
BseCI ATCGAT 1 cut(s) 2119
BseGI GGATG 4 cut(s) 89, 1017, 1320, 1758
BseLI CCNNNNNNNGG 6 cut(s) 1128, 1158, 1595, 2046, 2090, 2192
BseMII CTCAG 8 cut(s) 666, 899, 1114, 1137, 1242, 1821, 2279, 2292
BseNI ACTGG 5 cut(s) 25, 190, 451, 1182, 1470
BseRI GAGGAG 2 cut(s) 756, 873
BseSI GKGCMC 2 cut(s) 751, 1426
BseYI CCCAGC 2 cut(s) 1588, 1696
BsgI GTGCAG 1 cut(s) 1464
BshFI GGCC 5 cut(s) 366, 457, 1220, 1512, 2142
BshNI GGYRCC 1 cut(s) 748
BshVI ATCGAT 1 cut(s) 2119
BsiHKAI GWGCWC 2 cut(s) 577, 1426
BsiHKCI CYCGRG 2 cut(s) 204, 982
BsiSI CCGG 3 cut(s) 296, 983, 1529
BslFI GGGAC 7 cut(s) 659, 1337, 1482, 1570, 1678, 2065, 2096
BslI CCNNNNNNNGG 6 cut(s) 1128, 1158, 1595, 2046, 2090, 2192
BsmAI GTCTC 5 cut(s) 870, 885, 1103, 1109, 1926
BsmFI GGGAC 7 cut(s) 659, 1337, 1482, 1570, 1678, 2065, 2096
BsmI GAATGC 3 cut(s) 1793, 1955, 2201
BsnI GGCC 5 cut(s) 366, 457, 1220, 1512, 2142
BsoBI CYCGRG 2 cut(s) 204, 982
Bsp119I TTCGAA 2 cut(s) 323, 1110
Bsp1286I GDGCHC 3 cut(s) 577, 751, 1426
Bsp143I GATC 4 cut(s) 920, 1668, 1709, 2231
Bsp19I CCATGG 3 cut(s) 384, 1007, 1632
BspACI CCGC 4 cut(s) 543, 818, 1662, 2075
BspANI GGCC 5 cut(s) 366, 457, 1220, 1512, 2142
BspCNI CTCAG 8 cut(s) 665, 898, 1113, 1136, 1243, 1820, 2280, 2291
BspDI ATCGAT 1 cut(s) 2119
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
BspMI ACCTGC 1 cut(s) 1486
BspPI GGATC 1 cut(s) 928
BspT104I TTCGAA 2 cut(s) 323, 1110
BspT107I GGYRCC 1 cut(s) 748
BsrI ACTGG 5 cut(s) 25, 190, 451, 1182, 1470
BssMI GATC 4 cut(s) 920, 1668, 1709, 2231
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 906
BssSI CACGAG 2 cut(s) 1485, 1929
BssT1I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Bst1107I GTATAC 1 cut(s) 772
Bst2BI CACGAG 2 cut(s) 1485, 1929
Bst2UI CCWGG 4 cut(s) 352, 1560, 1842, 2252
Bst4CI ACNGT 7 cut(s) 224, 625, 1021, 1190, 1996, 2035, 2161
Bst6I CTCTTC 2 cut(s) 603, 1112
BstACI GRCGYC 1 cut(s) 906
BstBAI YACGTR 4 cut(s) 697, 1421, 1876, 2225
BstBI TTCGAA 2 cut(s) 323, 1110
BstC8I GCNNGC 7 cut(s) 171, 335, 501, 1275, 1452, 1639, 1953
BstDEI CTNAG 8 cut(s) 652, 885, 1100, 1123, 1251, 1807, 2278, 2288
BstDSI CCRYGG 3 cut(s) 384, 1007, 1632
BstEII GGTNACC 1 cut(s) 636
BstENI CCTNNNNNAGG 1 cut(s) 1126
BstF5I GGATG 4 cut(s) 89, 1017, 1320, 1758
BstKTI GATC 4 cut(s) 923, 1671, 1712, 2234
BstMAI GTCTC 5 cut(s) 870, 885, 1103, 1109, 1926
BstMBI GATC 4 cut(s) 920, 1668, 1709, 2231
BstMWI GCNNNNNNNGC 8 cut(s) 496, 680, 734, 755, 764, 1148, 1865, 2186
BstNI CCWGG 4 cut(s) 352, 1560, 1842, 2252
BstNSI RCATGY 4 cut(s) 490, 783, 1649, 1947
BstPAI GACNNNNGTC 1 cut(s) 1842
BstPI GGTNACC 1 cut(s) 636
BstSCI CCNGG 7 cut(s) 295, 350, 981, 982, 1558, 1840, 2250
BstSFI CTRYAG 4 cut(s) 621, 678, 1725, 2065
BstSLI GKGCMC 2 cut(s) 751, 1426
BstSNI TACGTA 1 cut(s) 1876
BstX2I RGATCY 1 cut(s) 1709
BstXI CCANNNNNNTGG 2 cut(s) 507, 2251
BstYI RGATCY 1 cut(s) 1709
BstZ17I GTATAC 1 cut(s) 772
Bsu15I ATCGAT 1 cut(s) 2119
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 5 cut(s) 366, 457, 1220, 1512, 2142
BsuTUI ATCGAT 1 cut(s) 2119
BtgI CCRYGG 3 cut(s) 384, 1007, 1632
BtsCI GGATG 4 cut(s) 89, 1017, 1320, 1758
BtsIMutI CAGTG 6 cut(s) 18, 630, 907, 1017, 2001, 2040
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 7 cut(s) 171, 335, 501, 1275, 1452, 1639, 1953
CaiI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
CciI TCATGA 1 cut(s) 1719
Cfr13I GGNCC 7 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768
Cfr9I CCCGGG 1 cut(s) 982
ClaI ATCGAT 1 cut(s) 2119
CseI GACGC 2 cut(s) 914, 1865
Csp6I GTAC 4 cut(s) 694, 1626, 1941, 2222
CviQI GTAC 4 cut(s) 694, 1626, 1941, 2222
DdeI CTNAG 8 cut(s) 652, 885, 1100, 1123, 1251, 1807, 2278, 2288
DpnI GATC 4 cut(s) 922, 1670, 1711, 2233
DpnII GATC 4 cut(s) 920, 1668, 1709, 2231
DraIII CACNNNGTG 1 cut(s) 1487
DriI GACNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 2140
Eam1104I CTCTTC 2 cut(s) 603, 1112
Eam1105I GACNNNNNGTC 1 cut(s) 856
EarI CTCTTC 2 cut(s) 603, 1112
Eco105I TACGTA 1 cut(s) 1876
Eco130I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 5 cut(s) 133, 872, 1584, 1692, 1768
Eco57I CTGAAG 3 cut(s) 223, 1167, 1794
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 2 cut(s) 204, 982
Eco91I GGTNACC 1 cut(s) 636
EcoNI CCTNNNNNAGG 1 cut(s) 1126
EcoO109I RGGNCCY 3 cut(s) 133, 872, 1511
EcoO65I GGTNACC 1 cut(s) 636
EcoRI GAATTC 2 cut(s) 945, 1880
EcoRII CCWGG 4 cut(s) 350, 1558, 1840, 2250
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
EcoT22I ATGCAT 1 cut(s) 2203
ErhI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
FaqI GGGAC 7 cut(s) 659, 1337, 1482, 1570, 1678, 2065, 2096
FbaI TGATCA 1 cut(s) 1668
FblI GTMKAC 3 cut(s) 771, 1243, 1851
FokI GGATG 4 cut(s) 76, 1024, 1327, 1745
GsaI CCCAGC 2 cut(s) 1592, 1700
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 5 cut(s) 366, 457, 1220, 1512, 2142
HapII CCGG 3 cut(s) 296, 983, 1529
HgaI GACGC 2 cut(s) 914, 1865
Hin1I GRCGYC 1 cut(s) 906
HincII GTYRAC 5 cut(s) 261, 615, 808, 850, 1244
HindII GTYRAC 5 cut(s) 261, 615, 808, 850, 1244
HindIII AAGCTT 3 cut(s) 273, 331, 1271
HinfI GANTC 9 cut(s) 27, 412, 461, 524, 611, 1265, 1656, 1830, 2094
HpaII CCGG 3 cut(s) 296, 983, 1529
HphI GGTGA 6 cut(s) 140, 206, 386, 532, 630, 1103
Hpy188I TCNGA 9 cut(s) 242, 839, 920, 1057, 1147, 1961, 1972, 2093, 2261
Hpy188III TCNNGA 8 cut(s) 528, 875, 966, 1312, 1440, 1720, 1739, 2235
Hpy99I CGWCG 1 cut(s) 1859
HpyAV CCTTC 8 cut(s) 505, 968, 1222, 1377, 1769, 1776, 1903, 2185
HpyCH4III ACNGT 7 cut(s) 224, 625, 1021, 1190, 1996, 2035, 2161
HpyCH4IV ACGT 5 cut(s) 696, 804, 1420, 1875, 2224
HpyF10VI GCNNNNNNNGC 8 cut(s) 496, 680, 734, 755, 764, 1148, 1865, 2186
HpyF3I CTNAG 8 cut(s) 652, 885, 1100, 1123, 1251, 1807, 2278, 2288
HpySE526I ACGT 5 cut(s) 696, 804, 1420, 1875, 2224
Hsp92I GRCGYC 1 cut(s) 906
Ksp22I TGATCA 1 cut(s) 1668
Kzo9I GATC 4 cut(s) 920, 1668, 1709, 2231
LmnI GCTCC 3 cut(s) 886, 1561, 2185
MaeII ACGT 5 cut(s) 696, 804, 1420, 1875, 2224
MalI GATC 4 cut(s) 922, 1670, 1711, 2233
MboI GATC 4 cut(s) 920, 1668, 1709, 2231
MboII GAAGA 8 cut(s) 389, 620, 954, 1099, 1494, 1791, 2119, 2158
MflI RGATCY 1 cut(s) 1709
MhlI GDGCHC 3 cut(s) 577, 751, 1426
MlyI GAGTC 3 cut(s) 620, 1274, 1839
MmeI TCCRAC 2 cut(s) 764, 1360
Mph1103I ATGCAT 1 cut(s) 2203
MseI TTAA 3 cut(s) 548, 701, 2123
MslI CAYNNNNRTG 2 cut(s) 860, 1763
MspA1I CMGCKG 2 cut(s) 728, 2075
MspI CCGG 3 cut(s) 296, 983, 1529
MspR9I CCNGG 7 cut(s) 297, 352, 983, 984, 1560, 1842, 2252
Mva1269I GAATGC 3 cut(s) 1793, 1955, 2201
MvaI CCWGG 4 cut(s) 352, 1560, 1842, 2252
MwoI GCNNNNNNNGC 8 cut(s) 496, 680, 734, 755, 764, 1148, 1865, 2186
NciI CCSGG 3 cut(s) 297, 983, 984
NcoI CCATGG 3 cut(s) 384, 1007, 1632
NdeII GATC 4 cut(s) 920, 1668, 1709, 2231
NlaIV GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 6 cut(s) 636, 665, 902, 1437, 1549, 2035
NsiI ATGCAT 1 cut(s) 2203
NspI RCATGY 4 cut(s) 490, 783, 1649, 1947
NspV TTCGAA 2 cut(s) 323, 1110
PagI TCATGA 1 cut(s) 1719
PciI ACATGT 3 cut(s) 779, 1645, 1943
PctI GAATGC 3 cut(s) 1793, 1955, 2201
PfeI GAWTC 6 cut(s) 27, 412, 461, 524, 1656, 2094
PfoI TCCNGGA 1 cut(s) 1558
PleI GAGTC 3 cut(s) 619, 1273, 1838
PpsI GAGTC 3 cut(s) 619, 1273, 1838
Ppu21I YACGTR 4 cut(s) 697, 1421, 1876, 2225
PpuMI RGGWCCY 2 cut(s) 133, 872
PscI ACATGT 3 cut(s) 779, 1645, 1943
PshAI GACNNNNGTC 1 cut(s) 1842
PshBI ATTAAT 1 cut(s) 2123
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 2 cut(s) 133, 872
Psp6I CCWGG 4 cut(s) 350, 1558, 1840, 2250
PspEI GGTNACC 1 cut(s) 636
PspFI CCCAGC 2 cut(s) 1588, 1696
PspGI CCWGG 4 cut(s) 350, 1558, 1840, 2250
PspN4I GGNNCC 6 cut(s) 135, 750, 1513, 1586, 1694, 1770
PspPI GGNCC 7 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768
PspPPI RGGWCCY 2 cut(s) 133, 872
PstNI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
PsuI RGATCY 1 cut(s) 1709
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 4 cut(s) 695, 1627, 1942, 2223
RsaNI GTAC 4 cut(s) 694, 1626, 1941, 2222
RseI CAYNNNNRTG 2 cut(s) 860, 1763
SalI GTCGAC 1 cut(s) 1242
SaqAI TTAA 3 cut(s) 548, 701, 2123
Sau3AI GATC 4 cut(s) 920, 1668, 1709, 2231
Sau96I GGNCC 7 cut(s) 133, 364, 872, 1511, 1584, 1692, 1768
SchI GAGTC 3 cut(s) 620, 1274, 1839
ScrFI CCNGG 7 cut(s) 297, 352, 983, 984, 1560, 1842, 2252
SduI GDGCHC 3 cut(s) 577, 751, 1426
SfcI CTRYAG 4 cut(s) 621, 678, 1725, 2065
SfuI TTCGAA 2 cut(s) 323, 1110
SinI GGWCC 5 cut(s) 133, 872, 1584, 1692, 1768
SmaI CCCGGG 1 cut(s) 984
SmiMI CAYNNNNRTG 2 cut(s) 860, 1763
SnaBI TACGTA 1 cut(s) 1876
SsiI CCGC 4 cut(s) 543, 818, 1662, 2075
StyD4I CCNGG 7 cut(s) 295, 350, 981, 982, 1558, 1840, 2250
StyI CCWWGG 6 cut(s) 93, 384, 641, 1007, 1215, 1632
TaaI ACNGT 7 cut(s) 224, 625, 1021, 1190, 1996, 2035, 2161
TaiI ACGT 5 cut(s) 699, 807, 1423, 1878, 2227
TaqI TCGA 6 cut(s) 323, 577, 1110, 1243, 1797, 2119
TatI WGTACW 1 cut(s) 1940
TauI GCSGC 2 cut(s) 821, 1664
TfiI GAWTC 6 cut(s) 27, 412, 461, 524, 1656, 2094
Tru1I TTAA 3 cut(s) 548, 701, 2123
Tru9I TTAA 3 cut(s) 548, 701, 2123
TscAI CASTG 6 cut(s) 25, 630, 907, 1024, 2001, 2040
TseFI GTSAC 6 cut(s) 636, 665, 902, 1437, 1549, 2035
Tsp45I GTSAC 6 cut(s) 636, 665, 902, 1437, 1549, 2035
TspDTI ATGAA 9 cut(s) 78, 156, 177, 349, 427, 612, 917, 987, 1130
TspGWI ACGGA 3 cut(s) 1052, 1161, 1277
TspMI CCCGGG 1 cut(s) 982
TspRI CASTG 6 cut(s) 25, 630, 907, 1024, 2001, 2040
VneI GTGCAC 1 cut(s) 1422
VpaK11BI GGWCC 5 cut(s) 133, 872, 1584, 1692, 1768
VspI ATTAAT 1 cut(s) 2123
XagI CCTNNNNNAGG 1 cut(s) 1126
XapI RAATTY 8 cut(s) 144, 282, 450, 536, 945, 1401, 1443, 1880
XceI RCATGY 4 cut(s) 490, 783, 1649, 1947
XmaI CCCGGG 1 cut(s) 982
XmiI GTMKAC 3 cut(s) 771, 1243, 1851
Zsp2I ATGCAT 1 cut(s) 2203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.