RLG00000016658

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016727
Physical Location & Seq
Reverse (-)
8629515 .. 8633016
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016658

Sequence Viewer

Length: 2292 bp
ATGGCGTCTTTTTGTTCTGCTTGGAGAAGAAGCAGTCTTCTTTTCATGCTGCTTTGTGTTCTTGTTGCTCAAATTAGCACTTGTTTTTCATCCGAGGTGTATGTGGTTTATTTGGGAAGCAAAAATGGTGAAGACCCAGATGAGATTCTGGCGCAAAACCATCAAATACTTGCTTCTGTTCACACAGGAAGCATAGAGGAAGCTCAAGCATCTCACATTCATAGTTACCGACATGGTTTTAAAGGCTTTGCTGCCAGGTTGACTGATCAGCAAGCTTTCCAAATTTCAAAGATGCCTGGAGTTGTTTCGGTTTTTCCCAACTCGAAAAGGAGTTTGCACACCACTCATTCTTGGGATTTCATGGGTCTTTTGGGTGAACAGACCTTGGAGGTTTCCGGCTTTAACATCAAGAATCAAGTAAATGTCATTGTCGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCAAGTTTTAATGATGACAACATGCCTCCAGTGCCAGCTAGCTGGAAGGGGGTTTGTCAATCAGGAGAGGCATTCAATGCTTCAACTTGCAACAGAAAAGTGATTGGGGCTAGATACTATAAGTCCGGTTATGAAGCCGAGGAAGATTCAGCGGACACAGTGGCATTCAGTTCTCCAAGGGACAGTGCCGGTCATGGCAGCCACACAGCCTCTATAGCTGCTGGTCGTTATGTGTCAAACATGACTTATAAGGGATTGGCATCTGGAGGAGCTAGAGGAGGTGCACCAATGGCCAGAATTGCAGTATATAAAACTTGTTGGGACACTGGTTGTTATGACGTAGACTTACTAGCAGCTTTTGATGATGCAATTAGAGATGGGGTTCACATTCTGTCTCTGTCTCTCGGGCCTGATGCTCCTCAGGGAGACTATTTCACTGATGCCATTTCTGTGGGGTCATTCCATGCTGCTAGGCATGGAATTCTAGTGGTTGCTTCTGCTGGAAATGAAGGATACCCGGGTTCTGCAACAAATCTCGCCCCATGGATGATTACTGTTGCTGCAAGTTCAACAGATAGGGATTTCACTTCTGATATCGTTCTTGGAAATGGTGTTAATGTCACGGGTGAAAGCCTCAGTGTATTTGGAATGAAAGCCTCAGCAAGCATCATTTCCGCCTCTGAAGCCTTTGCAGGGTACTTCACTCCATATCAATCCAGTTATTGTTTAGAAAGTTCCTTGAACAAGACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGTCATGCTGAGAGTTCAACAGAGTCGAAGCTTGCAAAAAGTGTAGTAGTTAAAGAAGCTGGTGGTGTCGGGATGGTTCTTATCGATGAGGCAGATATGGATGTTGCCGTCCCTTTTGTTATCCCTTCAGCAATCATTAGACGTAGGATAGGAAAACATCTTCTATCTTACATTAATCGCACACGCCAACCAATGTCGAGAATTCTCCCTGCAAAGACTACACTGGGATTGAAACCTGCACCTCATGTTACAGCATTTTCTGCAAAAGGTCCCAATGGTTTGACACCAGAAATTTTGAAGCCTGATATCACAGCTCCTGGACTGAACATACTTGCATCTTGGTCCCCAGCAGTAGCTGGTAAGCAGTTCAACATTCTTTCTGGTACATCCATGGCTTGCCCGCATGTAACAGGAATTGCCACCTTGATAAAAGCCATTCATCCTTCATGGTCCCCAGCAGCTATTAGATCGGCAATCATGACTACTGCTACTCTTCTGGACAAGAAGCACAAGCCGATTACAGTGGACCCTGAAGGCAAAAAGGGTAATGCATTTGATTATGGCTCAGGCTTCCTGAACCCAACAAGAGCACTTGATCCAGGTCTTGTCTTTGACGCACAGCCAGCAGATTATGTATCATTCCTTTGTTCAGTTGGATATGATGAGAAGTCATTGCATCAAATCACACTAGACAACAGTACATGCGCTCAGTCATTCAGAACAGCATCTGACCTTAACTATCCATCTATCACTGTACCAAATCTCGAAGGGAACATCTCAGTAACTAGAACAGTGACCAACGTCGGGAAGCCAAAGAATATCTATAAAGCCGCTGTGTCCTCACCCAAAGGAATCAATGTTACTGTTATCCCAAATCGACTGGTCTTCACCAGCCTTGGTCAGAAGCTAAGCTTCACTGTGAATTTCAAGGTGGTTGCTCCATCAAAGGGATATGCATTTGGGTTCTTGTCATGGATGAGTGGAAGATCGAGGGTTACCAGTCCTCTCGTTGTCCAAGTTGCGCATTCAAACTCAGGGCTGATGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

764

Amino Acids

81.4

Weight (kDa)

8.72

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 6.2e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 601 5.2e-45 Subtilase family
fn3_6 PF17766 658 - 752 2.2e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc16I TGCGCA 1 cut(s) 2265
Acc36I ACCTGC 1 cut(s) 1486
AccI GTMKAC 1 cut(s) 807
AciI CCGC 4 cut(s) 617, 1140, 1643, 2073
AclWI GGATC 1 cut(s) 1832
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 6 cut(s) 282, 450, 945, 1443, 1533, 2164
AcuI CTGAAG 3 cut(s) 1167, 1353, 1794
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 1163, 1627, 1942, 1998
AfiI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
AjnI CCWGG 4 cut(s) 254, 295, 1558, 1840
Alw21I GWGCWC 2 cut(s) 751, 1834
Alw26I GTCTC 3 cut(s) 864, 870, 885
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 1832
AlwNI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
Ama87I CYCGRG 2 cut(s) 869, 982
AoxI GGCC 4 cut(s) 455, 756, 872, 1218
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 8 cut(s) 49, 251, 663, 683, 818, 932, 1025, 1700
ApoI RAATTY 6 cut(s) 282, 450, 945, 1443, 1533, 2164
AseI ATTAAT 1 cut(s) 1416
AspLEI GCGC 3 cut(s) 154, 1949, 2266
AspS9I GGNCC 5 cut(s) 872, 1511, 1584, 1692, 1768
AsuC2I CCSGG 2 cut(s) 983, 984
AsuHPI GGTGA 5 cut(s) 140, 386, 1103, 2076, 2122
AsuNHI GCTAGC 1 cut(s) 503
AvaI CYCGRG 2 cut(s) 869, 982
AvaII GGWCC 4 cut(s) 1511, 1584, 1692, 1768
AxyI CCTNAGG 1 cut(s) 885
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BalI TGGCCA 1 cut(s) 758
BbsI GAAGAC 3 cut(s) 29, 138, 2119
Bbv12I GWGCWC 2 cut(s) 751, 1834
BbvCI CCTCAGC 1 cut(s) 1123
BbvI GCAGC 8 cut(s) 36, 238, 670, 675, 830, 919, 1012, 1712
BccI CCATC 5 cut(s) 168, 836, 1309, 1993, 2191
BceAI ACGGC 1 cut(s) 1334
BciT130I CCWGG 4 cut(s) 256, 297, 1560, 1842
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 2 cut(s) 983, 984
BcoDI GTCTC 3 cut(s) 864, 870, 885
BfaI CTAG 8 cut(s) 504, 576, 738, 815, 936, 950, 1931, 2028
BfmI CTRYAG 1 cut(s) 678
BfuAI ACCTGC 1 cut(s) 1486
BfuI GTATCC 1 cut(s) 971
BisI GCNGC 9 cut(s) 50, 252, 664, 684, 819, 933, 1026, 1701, 2073
BlpI GCTNAGC 1 cut(s) 2150
BlsI GCNGC 9 cut(s) 51, 253, 665, 685, 820, 934, 1027, 1702, 2074
Bme1390I CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
Bme18I GGWCC 4 cut(s) 1511, 1584, 1692, 1768
BmeT110I CYCGRG 2 cut(s) 869, 982
BmgT120I GGNCC 5 cut(s) 872, 1511, 1584, 1692, 1768
BmiI GGNNCC 4 cut(s) 1513, 1586, 1694, 1770
BmrFI CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
BmrI ACTGGG 1 cut(s) 1475
BmtI GCTAGC 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 1475
BoxI GACNNNNGTC 1 cut(s) 2042
BpiI GAAGAC 3 cut(s) 29, 138, 2119
BpmI CTGGAG 3 cut(s) 318, 477, 750
Bpu10I CCTNAGC 2 cut(s) 1123, 1807
Bpu1102I GCTNAGC 1 cut(s) 2150
BpuEI CTTGAG 1 cut(s) 189
BpuMI CCSGG 2 cut(s) 983, 984
Bsa29I ATCGAT 1 cut(s) 1326
BsaBI GATNNNNATC 1 cut(s) 724
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BsaWI WCCGGW 1 cut(s) 590
Bsc4I CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 7 cut(s) 451, 494, 796, 1182, 1470, 2127, 2241
Bse21I CCTNAGG 1 cut(s) 885
Bse3DI GCAATG 1 cut(s) 1913
Bse8I GATNNNNATC 1 cut(s) 724
BseBI CCWGG 4 cut(s) 256, 297, 1560, 1842
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BseGI GGATG 7 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2223
BseJI GATNNNNATC 1 cut(s) 724
BseLI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
BseMI GCAATG 1 cut(s) 1913
BseMII CTCAG 8 cut(s) 899, 1114, 1137, 1242, 1821, 1964, 2034, 2289
BseNI ACTGG 7 cut(s) 451, 494, 796, 1182, 1470, 2127, 2241
BseRI GAGGAG 3 cut(s) 747, 756, 873
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 8 cut(s) 36, 238, 670, 675, 830, 919, 1012, 1712
BseYI CCCAGC 2 cut(s) 1588, 1696
BsgI GTGCAG 1 cut(s) 1464
BshFI GGCC 4 cut(s) 457, 758, 874, 1220
BshVI ATCGAT 1 cut(s) 1326
BsiHKAI GWGCWC 2 cut(s) 751, 1834
BsiHKCI CYCGRG 2 cut(s) 869, 982
BsiSI CCGG 4 cut(s) 396, 591, 654, 983
BslFI GGGAC 6 cut(s) 659, 800, 1337, 1497, 1570, 1678
BslI CCNNNNNNNGG 3 cut(s) 1158, 2046, 2189
BsmAI GTCTC 3 cut(s) 864, 870, 885
BsmFI GGGAC 6 cut(s) 659, 800, 1337, 1497, 1570, 1678
BsmI GAATGC 3 cut(s) 536, 629, 2266
BsnI GGCC 4 cut(s) 457, 758, 874, 1220
BsoBI CYCGRG 2 cut(s) 869, 982
Bsp1286I GDGCHC 2 cut(s) 751, 1834
Bsp143I GATC 4 cut(s) 265, 1709, 1837, 2228
Bsp1720I GCTNAGC 1 cut(s) 2150
Bsp19I CCATGG 2 cut(s) 1007, 1632
BspACI CCGC 4 cut(s) 617, 1140, 1643, 2073
BspANI GGCC 4 cut(s) 457, 758, 874, 1220
BspCNI CTCAG 8 cut(s) 898, 1113, 1136, 1243, 1820, 1963, 2033, 2288
BspDI ATCGAT 1 cut(s) 1326
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 4 cut(s) 1513, 1586, 1694, 1770
BspMI ACCTGC 1 cut(s) 1486
BspOI GCTAGC 1 cut(s) 507
BspPI GGATC 1 cut(s) 1832
BsrDI GCAATG 1 cut(s) 1913
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 7 cut(s) 451, 494, 796, 1182, 1470, 2127, 2241
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 9 cut(s) 93, 384, 603, 641, 982, 1007, 1215, 1632, 2137
BssMI GATC 4 cut(s) 265, 1709, 1837, 2228
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 6 cut(s) 384, 641, 1007, 1215, 1632, 2137
Bst2UI CCWGG 4 cut(s) 256, 297, 1560, 1842
Bst4CI ACNGT 9 cut(s) 625, 650, 1021, 1765, 1940, 1996, 2035, 2107, 2161
Bst6I CTCTTC 1 cut(s) 1740
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 542, 1502
BstC8I GCNNGC 8 cut(s) 273, 501, 505, 1129, 1275, 1639, 1643, 1866
BstDEI CTNAG 9 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2150, 2275
BstDSI CCRYGG 2 cut(s) 1007, 1632
BstEII GGTNACC 1 cut(s) 2236
BstF5I GGATG 7 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2223
BstHHI GCGC 3 cut(s) 154, 1949, 2266
BstKTI GATC 4 cut(s) 268, 1712, 1840, 2231
BstMAI GTCTC 3 cut(s) 864, 870, 885
BstMBI GATC 4 cut(s) 265, 1709, 1837, 2228
BstMWI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
BstNI CCWGG 4 cut(s) 256, 297, 1560, 1842
BstNSI RCATGY 3 cut(s) 490, 1649, 1947
BstPAI GACNNNNGTC 1 cut(s) 2042
BstPI GGTNACC 1 cut(s) 2236
BstSCI CCNGG 6 cut(s) 254, 295, 981, 982, 1558, 1840
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 8 cut(s) 36, 238, 670, 675, 830, 919, 1012, 1712
BstV2I GAAGAC 3 cut(s) 29, 138, 2119
BstXI CCANNNNNNTGG 2 cut(s) 507, 916
Bsu15I ATCGAT 1 cut(s) 1326
Bsu36I CCTNAGG 1 cut(s) 885
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 4 cut(s) 457, 758, 874, 1220
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 2 cut(s) 1007, 1632
BtsCI GGATG 7 cut(s) 89, 1017, 1320, 1348, 1628, 1681, 2223
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 8 cut(s) 273, 501, 505, 1129, 1275, 1639, 1643, 1866
CaiI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 3 cut(s) 154, 1949, 2266
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 5 cut(s) 872, 1511, 1584, 1692, 1768
Cfr9I CCCGGG 1 cut(s) 982
ClaI ATCGAT 1 cut(s) 1326
CseI GACGC 1 cut(s) 1865
Csp6I GTAC 4 cut(s) 1162, 1626, 1941, 1997
CviQI GTAC 4 cut(s) 1162, 1626, 1941, 1997
DdeI CTNAG 9 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2150, 2275
DpnI GATC 4 cut(s) 267, 1711, 1839, 2230
DpnII GATC 4 cut(s) 265, 1709, 1837, 2228
DraI TTTAAA 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 1740
EarI CTCTTC 1 cut(s) 1740
EciI GGCGGA 1 cut(s) 1129
Eco130I CCWWGG 6 cut(s) 384, 641, 1007, 1215, 1632, 2137
Eco32I GATATC 2 cut(s) 1060, 1549
Eco47I GGWCC 4 cut(s) 1511, 1584, 1692, 1768
Eco57I CTGAAG 3 cut(s) 1167, 1353, 1794
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 2 cut(s) 869, 982
Eco91I GGTNACC 1 cut(s) 2236
EcoO109I RGGNCCY 1 cut(s) 1511
EcoO65I GGTNACC 1 cut(s) 2236
EcoRI GAATTC 2 cut(s) 945, 1443
EcoRII CCWGG 4 cut(s) 254, 295, 1558, 1840
EcoRV GATATC 2 cut(s) 1060, 1549
EcoT14I CCWWGG 6 cut(s) 384, 641, 1007, 1215, 1632, 2137
EcoT22I ATGCAT 2 cut(s) 1795, 2200
ErhI CCWWGG 6 cut(s) 384, 641, 1007, 1215, 1632, 2137
FalI AAGNNNNNCTT 2 cut(s) 2138, 2170
FaqI GGGAC 6 cut(s) 659, 800, 1337, 1497, 1570, 1678
FauI CCCGC 1 cut(s) 1650
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 807
Fnu4HI GCNGC 9 cut(s) 50, 252, 664, 684, 819, 933, 1026, 1701, 2073
FokI GGATG 7 cut(s) 76, 1024, 1327, 1355, 1615, 1668, 2230
Fsp4HI GCNGC 9 cut(s) 50, 252, 664, 684, 819, 933, 1026, 1701, 2073
FspBI CTAG 8 cut(s) 504, 576, 738, 815, 936, 950, 1931, 2028
FspI TGCGCA 1 cut(s) 2265
GlaI GCGC 3 cut(s) 153, 1948, 2265
GluI GCNGC 9 cut(s) 50, 252, 664, 684, 819, 933, 1026, 1701, 2073
GsaI CCCAGC 2 cut(s) 1592, 1700
GsuI CTGGAG 3 cut(s) 318, 477, 750
HaeIII GGCC 4 cut(s) 457, 758, 874, 1220
HapII CCGG 4 cut(s) 396, 591, 654, 983
HgaI GACGC 1 cut(s) 1865
HhaI GCGC 3 cut(s) 154, 1949, 2266
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 152, 1947, 2264
HinP1I GCGC 3 cut(s) 152, 1947, 2264
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 3 cut(s) 273, 1271, 2152
HinfI GANTC 6 cut(s) 145, 412, 461, 611, 1265, 2094
HpaII CCGG 4 cut(s) 396, 591, 654, 983
HphI GGTGA 5 cut(s) 140, 386, 1103, 2076, 2122
Hpy166II GTNNAC 7 cut(s) 181, 261, 377, 749, 808, 850, 1768
Hpy188I TCNGA 6 cut(s) 94, 1057, 1147, 1961, 1972, 2145
Hpy8I GTNNAC 7 cut(s) 181, 261, 377, 749, 808, 850, 1768
Hpy99I CGWCG 1 cut(s) 2048
HpyAV CCTTC 7 cut(s) 505, 968, 1222, 1377, 1695, 1769, 2003
HpyCH4III ACNGT 9 cut(s) 625, 650, 1021, 1765, 1940, 1996, 2035, 2107, 2161
HpyCH4IV ACGT 3 cut(s) 804, 1384, 2043
HpyF10VI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
HpyF3I CTNAG 9 cut(s) 885, 1100, 1123, 1251, 1807, 1950, 2020, 2150, 2275
HpySE526I ACGT 3 cut(s) 804, 1384, 2043
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 152, 1947, 2264
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 265, 1709, 1837, 2228
LmnI GCTCC 4 cut(s) 734, 886, 1561, 2185
Lsp1109I GCAGC 8 cut(s) 36, 238, 670, 675, 830, 919, 1012, 1712
MaeI CTAG 8 cut(s) 504, 576, 738, 815, 936, 950, 1931, 2028
MaeII ACGT 3 cut(s) 804, 1384, 2043
MaeIII GTNAC 8 cut(s) 224, 1084, 1489, 1648, 2023, 2035, 2101, 2236
MalI GATC 4 cut(s) 267, 1711, 1839, 2230
MboI GATC 4 cut(s) 265, 1709, 1837, 2228
MboII GAAGA 8 cut(s) 29, 39, 143, 620, 1394, 1727, 2119, 2238
MhlI GDGCHC 2 cut(s) 751, 1834
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 1 cut(s) 1274
MmeI TCCRAC 1 cut(s) 1876
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 2 cut(s) 1795, 2200
MscI TGGCCA 1 cut(s) 758
MseI TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
MslI CAYNNNNRTG 1 cut(s) 914
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 2 cut(s) 617, 2075
MspI CCGG 4 cut(s) 396, 591, 654, 983
MspR9I CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
Mva1269I GAATGC 3 cut(s) 536, 629, 2266
MvaI CCWGG 4 cut(s) 256, 297, 1560, 1842
MwoI GCNNNNNNNGC 8 cut(s) 496, 542, 680, 755, 764, 1148, 1502, 1865
NciI CCSGG 2 cut(s) 983, 984
NcoI CCATGG 2 cut(s) 1007, 1632
NdeII GATC 4 cut(s) 265, 1709, 1837, 2228
NheI GCTAGC 1 cut(s) 503
NlaIV GGNNCC 4 cut(s) 1513, 1586, 1694, 1770
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 1084, 2035
NsbI TGCGCA 1 cut(s) 2265
NsiI ATGCAT 2 cut(s) 1795, 2200
NspI RCATGY 3 cut(s) 490, 1649, 1947
PagI TCATGA 1 cut(s) 1719
PctI GAATGC 3 cut(s) 536, 629, 2266
PfeI GAWTC 5 cut(s) 145, 412, 461, 611, 2094
PfoI TCCNGGA 1 cut(s) 1558
PkrI GCNGC 9 cut(s) 51, 253, 665, 685, 820, 934, 1027, 1702, 2074
PleI GAGTC 1 cut(s) 1273
PpsI GAGTC 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 1511
PshAI GACNNNNGTC 1 cut(s) 2042
PshBI ATTAAT 1 cut(s) 1416
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1511
Psp6I CCWGG 4 cut(s) 254, 295, 1558, 1840
PspEI GGTNACC 1 cut(s) 2236
PspFI CCCAGC 2 cut(s) 1588, 1696
PspGI CCWGG 4 cut(s) 254, 295, 1558, 1840
PspN4I GGNNCC 4 cut(s) 1513, 1586, 1694, 1770
PspPI GGNCC 5 cut(s) 872, 1511, 1584, 1692, 1768
PspPPI RGGWCCY 1 cut(s) 1511
PstNI CAGNNNCTG 3 cut(s) 1559, 1598, 1970
RsaI GTAC 4 cut(s) 1163, 1627, 1942, 1998
RsaNI GTAC 4 cut(s) 1162, 1626, 1941, 1997
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
SatI GCNGC 9 cut(s) 50, 252, 664, 684, 819, 933, 1026, 1701, 2073
Sau3AI GATC 4 cut(s) 265, 1709, 1837, 2228
Sau96I GGNCC 5 cut(s) 872, 1511, 1584, 1692, 1768
SchI GAGTC 1 cut(s) 1274
ScrFI CCNGG 6 cut(s) 256, 297, 983, 984, 1560, 1842
SduI GDGCHC 2 cut(s) 751, 1834
SfcI CTRYAG 1 cut(s) 678
SinI GGWCC 4 cut(s) 1511, 1584, 1692, 1768
SmaI CCCGGG 1 cut(s) 984
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
SsiI CCGC 4 cut(s) 617, 1140, 1643, 2073
SspMI CTAG 8 cut(s) 504, 576, 738, 815, 936, 950, 1931, 2028
StyD4I CCNGG 6 cut(s) 254, 295, 981, 982, 1558, 1840
StyI CCWWGG 6 cut(s) 384, 641, 1007, 1215, 1632, 2137
TaaI ACNGT 9 cut(s) 625, 650, 1021, 1765, 1940, 1996, 2035, 2107, 2161
TaiI ACGT 3 cut(s) 807, 1387, 2046
TaqI TCGA 7 cut(s) 323, 1268, 1326, 1439, 2007, 2119, 2231
TatI WGTACW 1 cut(s) 1940
TauI GCSGC 1 cut(s) 2075
TfiI GAWTC 5 cut(s) 145, 412, 461, 611, 2094
Tru1I TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
Tru9I TTAA 7 cut(s) 240, 402, 474, 1080, 1293, 1416, 1977
TseFI GTSAC 2 cut(s) 1084, 2035
TseI GCWGC 8 cut(s) 49, 251, 663, 683, 818, 932, 1025, 1700
Tsp45I GTSAC 2 cut(s) 1084, 2035
TspMI CCCGGG 1 cut(s) 982
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 4 cut(s) 1511, 1584, 1692, 1768
VspI ATTAAT 1 cut(s) 1416
XapI RAATTY 6 cut(s) 282, 450, 945, 1443, 1533, 2164
XceI RCATGY 3 cut(s) 490, 1649, 1947
XmaI CCCGGG 1 cut(s) 982
XmiI GTMKAC 1 cut(s) 807
XspI CTAG 8 cut(s) 504, 576, 738, 815, 936, 950, 1931, 2028
Zsp2I ATGCAT 2 cut(s) 1795, 2200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.