MD15G1226700.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016727
Physical Location & Seq
Reverse (-)
18420048 .. 18423974
3927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1226700.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGGGGTCTTTTTGTTGTAGTGGGAGAATTAGCAGCATTCTTCTTCTGTTCTTATGTGTTCTTGCTGCTGAAATTAGCATTTGCCTTTCATCCAAGGTGTATGTTGTTTATATGGGAAGCAAAAATGGTGAGGATCCAGATGAGATTTTGACACAAAACCATGAAATGCTTGCTTCTGTGCACACTGGAAGCATGGAGGAAGCTCAGGCATCTCACATTTACAGTTACAGACATGGCTTCAGAGGCTTTGCTGCCAAGTTAACCGACCTCCAAGCATTCCAAATTTCCCAGATGCCAGGAGTTGTTTCGGTTTTCCCCAATTCGAAAAGAAGCTTGCACACCACTCATTCCTGGGATTTCATGGGCCTTTTGGGTGAAGAAACTATGGAGATTTCTGGGTTTTCCACCAAGAACCAAGTTAATGTCATTGTTGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCGAGTTTTGATGATGCCAACATGCCTCCGGTGCCAGCTAGATGGAAGGGGCGTTGTCAATTAGGAGAAAAATTCACTGCTTCAACTTGCAACAGGAAAGTGATTGGCGCTCGATACTATAATTCTGGTTATGAAGCTGAGGAAGAGTCAACAACCAGAGTGTCATTCAGATCACCAAGGGACAGCTCCGGTCATGGCAGTCACACAGCCTCTATAGCTGCTGGTCGGTATGTGTCAAACATGACTTATAAGGGATTGGCAGCTGGAGGGGCAAGAGGAGGTGCACCAATGGCTAGGATTGCAGTGTACAAGACATGCTGGAACTCTGGTTGTTATGACGTTGACCTACTAGCGGCCTTTGATGATGCAATCAGAGACGGGGTTGATATCATGTCTCTGTCTCTAGGTCCTGATGCTCCTCAGGGTGACTATTTCAGTGATGCCATTTCTGTCGGATCATTTCATGCTGCTAGTCATGGAATTTTAGTGGTTGCTTCTTCTGGAAATGAAGGAAACCCCGGTTCTGCAACAAATTTAGCCCCATGGATGCTCACTGTTGCAGCAAGTTCAACAGATAGGGATTTCACTTCTGATATCATACTAGAAAATGGTGCTAATTTCACGGGTGAAAGTCTCAGTCTCTTCGAAATGAAAACCTCAGCAAGAATTATTTCCGCCTCTGAAGCCAATGCAGGGTATTTTACTCCGTATCAATCCAGTTACTGTTTAGAAAGTTCCTTGAATAGAACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGACATGCTGAGAGTTCAACAGAGTCAAAGCTCACTAAAAGTATGCTAGTGAAAAAAGCTGGTGGTGTCGGGATGGTTCTCATTGATGGGGCAGATAAGGGTATTGCTGTCCCATTTGTCATCCCTTCAGCTATTGTTGAACAGAGGATAGGGCATCGTATTTTATCTTATATTAAACGCACACGGAAACCCATGTCTCGAATTTTGCCCGCAAAGACTATACTGGGACTGAAACCTGCACCTCGTGTTACTGCGTTTTCTTCAAAAGGCCCCAATTCATTGACACCGGAGATTTTGAAGCCCGATGTCACAGCTCCTGGACTGAATATACTAGCATCTTGGTCCCCAGCAGCAGGGAATAAGCAGTTCAACATTCTTTCTGGTACTTCCATGGCTTGCCCACATGTAACAGGAATCGCCGCCTTGATCAAAGCTGTTTATCCATCATGGTCCCCAGCAGCTATTAGATCTGCAATCATGACTACGGCTACTCTTCTGGATAAGAACCACAAACCCATCCTAGTGGACCCTGAAGGAAGAAGGGGTAACGCATTCGATTATGGCTCAGGCTTTGTGAACCCGAAAAGAGTCCTTGACCCCGGCCTTGTCTATGACGTGCATTCAGCAGACTACGTAGAATTCCTTTGTTCAGTTGGTTATGACGAGAAGGCAGTCCATCAAATCACACAAGACAACAGTACGTGTAAGCAGGCATTTCGAACAGCGTCTGACCTGAACTATCCATCTATCACAGTGCCATATCTTGTAGGTAACTTCTCGGTAACTAGAACAGTGACCAATGTTGGTAAGCCAAAGCGTATATATAAAGCTGTGGTGTCCTCACCCGTTGGAATCAGTGTTACTATTGTCCCGAATCGATTAATCTTCAAGAGGTTTGGCCAGAAGATACAGTTCACTGTAAACTTCAAGGTGGCTGCTCCACCATCGAAGGGATATGCATTCGGGTTCTTTTCATGGGTAAGTGGACGATCAAGAGTTACCAGCCCCCTGGTTGTCCGAGTTGCACATTCAAACTCAGGGCTGCTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

765

Amino Acids

82.26

Weight (kDa)

9.17

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 1.9e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 585 7.1e-49 Subtilase family
fn3_6 PF17766 658 - 753 1e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 1486
AccB1I GGYRCC 1 cut(s) 496
AccI GTMKAC 1 cut(s) 1243
AciI CCGC 4 cut(s) 818, 1140, 1452, 1662
AclWI GGATC 3 cut(s) 128, 141, 928
AcoI YGGCCR 1 cut(s) 2140
AcsI RAATTY 7 cut(s) 282, 450, 536, 945, 997, 1443, 1880
AcuI CTGAAG 4 cut(s) 223, 1167, 1353, 1794
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 3 cut(s) 773, 1627, 1942
AfiI CCNNNNNNNGG 4 cut(s) 817, 1158, 1595, 2192
AflIII ACRYGT 2 cut(s) 1645, 1943
AjiI CACGTC 1 cut(s) 1858
AjnI CCWGG 4 cut(s) 295, 350, 1558, 2250
Alw21I GWGCWC 2 cut(s) 183, 751
Alw26I GTCTC 6 cut(s) 834, 864, 870, 1103, 1109, 1443
Alw44I GTGCAC 2 cut(s) 179, 747
AlwI GGATC 3 cut(s) 128, 141, 928
AlwNI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
AoxI GGCC 7 cut(s) 364, 455, 819, 1218, 1510, 1843, 2140
ApaLI GTGCAC 2 cut(s) 179, 747
ApoI RAATTY 7 cut(s) 282, 450, 536, 945, 997, 1443, 1880
AseI ATTAAT 1 cut(s) 2123
Asp700I GAANNNNTTC 1 cut(s) 1135
AspLEI GCGC 1 cut(s) 575
AspS9I GGNCC 6 cut(s) 364, 872, 1511, 1584, 1692, 1768
AsuC2I CCSGG 2 cut(s) 984, 1842
AsuHPI GGTGA 6 cut(s) 140, 386, 630, 902, 1103, 2076
AsuII TTCGAA 3 cut(s) 323, 1110, 1960
AvaII GGWCC 4 cut(s) 872, 1584, 1692, 1768
AxyI CCTNAGG 1 cut(s) 885
BaeGI GKGCMC 2 cut(s) 183, 751
BalI TGGCCA 1 cut(s) 2142
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 496
BauI CACGAG 1 cut(s) 1485
Bbv12I GWGCWC 2 cut(s) 183, 751
BbvCI CCTCAGC 2 cut(s) 603, 1123
BccI CCATC 8 cut(s) 501, 1309, 1322, 1693, 1766, 1926, 1993, 2194
BceAI ACGGC 1 cut(s) 1743
BcgI CGANNNNNNTGC 2 cut(s) 1940, 1974
BciT130I CCWGG 4 cut(s) 297, 352, 1560, 2252
BclI TGATCA 1 cut(s) 1668
BcnI CCSGG 2 cut(s) 984, 1842
BcoDI GTCTC 6 cut(s) 834, 864, 870, 1103, 1109, 1443
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 1486
BglII AGATCT 1 cut(s) 1709
Bme1390I CCNGG 6 cut(s) 297, 352, 984, 1560, 1842, 2252
Bme18I GGWCC 4 cut(s) 872, 1584, 1692, 1768
BmgBI CACGTC 1 cut(s) 1858
BmgT120I GGNCC 6 cut(s) 364, 872, 1511, 1584, 1692, 1768
BmiI GGNNCC 6 cut(s) 135, 498, 1513, 1586, 1694, 1770
BmrFI CCNGG 6 cut(s) 297, 352, 984, 1560, 1842, 2252
BmrI ACTGGG 1 cut(s) 1475
BmsI GCATC 9 cut(s) 218, 282, 469, 820, 868, 895, 1002, 1405, 1586
BmuI ACTGGG 1 cut(s) 1475
BpmI CTGGAG 1 cut(s) 750
Bpu10I CCTNAGC 4 cut(s) 204, 603, 1123, 1807
Bpu14I TTCGAA 3 cut(s) 323, 1110, 1960
BpuMI CCSGG 2 cut(s) 984, 1842
Bsa29I ATCGAT 1 cut(s) 2119
BsaAI YACGTR 2 cut(s) 1876, 1944
BsaJI CCNNGG 9 cut(s) 93, 351, 641, 982, 1007, 1215, 1632, 1840, 2250
BsaWI WCCGGW 3 cut(s) 493, 653, 1528
Bsc4I CCNNNNNNNGG 4 cut(s) 817, 1158, 1595, 2192
Bse1I ACTGG 4 cut(s) 190, 451, 1182, 1470
Bse21I CCTNAGG 1 cut(s) 885
BseBI CCWGG 4 cut(s) 297, 352, 1560, 2252
BseCI ATCGAT 1 cut(s) 2119
BseDI CCNNGG 9 cut(s) 93, 351, 641, 982, 1007, 1215, 1632, 1840, 2250
BseGI GGATG 5 cut(s) 89, 1017, 1320, 1362, 1758
BseLI CCNNNNNNNGG 4 cut(s) 817, 1158, 1595, 2192
BseMII CTCAG 9 cut(s) 218, 594, 899, 1114, 1137, 1242, 1821, 2279, 2292
BseNI ACTGG 4 cut(s) 190, 451, 1182, 1470
BseRI GAGGAG 2 cut(s) 756, 873
BseSI GKGCMC 2 cut(s) 183, 751
BseYI CCCAGC 2 cut(s) 1588, 1696
BsgI GTGCAG 1 cut(s) 1464
BshFI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1845, 2142
BshNI GGYRCC 1 cut(s) 496
BshVI ATCGAT 1 cut(s) 2119
BsiHKAI GWGCWC 2 cut(s) 183, 751
BsiSI CCGG 5 cut(s) 494, 654, 984, 1529, 1842
BslFI GGGAC 6 cut(s) 659, 1337, 1482, 1570, 1678, 2096
BslI CCNNNNNNNGG 4 cut(s) 817, 1158, 1595, 2192
BsmAI GTCTC 6 cut(s) 834, 864, 870, 1103, 1109, 1443
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 6 cut(s) 659, 1337, 1482, 1570, 1678, 2096
BsmI GAATGC 5 cut(s) 36, 275, 1793, 1861, 2201
BsnI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1845, 2142
Bsp119I TTCGAA 3 cut(s) 323, 1110, 1960
Bsp1286I GDGCHC 2 cut(s) 183, 751
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
Bsp19I CCATGG 2 cut(s) 1007, 1632
BspACI CCGC 4 cut(s) 818, 1140, 1452, 1662
BspANI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1845, 2142
BspCNI CTCAG 9 cut(s) 217, 595, 898, 1113, 1136, 1243, 1820, 2280, 2291
BspDI ATCGAT 1 cut(s) 2119
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 6 cut(s) 135, 498, 1513, 1586, 1694, 1770
BspMI ACCTGC 1 cut(s) 1486
BspPI GGATC 3 cut(s) 128, 141, 928
BspT104I TTCGAA 3 cut(s) 323, 1110, 1960
BspT107I GGYRCC 1 cut(s) 496
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 4 cut(s) 190, 451, 1182, 1470
BssECI CCNNGG 9 cut(s) 93, 351, 641, 982, 1007, 1215, 1632, 1840, 2250
BssMI GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
BssSI CACGAG 1 cut(s) 1485
BssT1I CCWWGG 5 cut(s) 93, 641, 1007, 1215, 1632
Bst2BI CACGAG 1 cut(s) 1485
Bst2UI CCWGG 4 cut(s) 297, 352, 1560, 2252
Bst4CI ACNGT 8 cut(s) 224, 1021, 1190, 1940, 1996, 2035, 2154, 2161
Bst6I CTCTTC 3 cut(s) 603, 1112, 1740
BstAUI TGTACA 1 cut(s) 771
BstBAI YACGTR 2 cut(s) 1876, 1944
BstBI TTCGAA 3 cut(s) 323, 1110, 1960
BstC8I GCNNGC 6 cut(s) 171, 335, 501, 1452, 1639, 1953
BstDEI CTNAG 9 cut(s) 204, 603, 885, 1100, 1123, 1251, 1807, 2278, 2288
BstDSI CCRYGG 2 cut(s) 1007, 1632
BstF5I GGATG 5 cut(s) 89, 1017, 1320, 1362, 1758
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 1 cut(s) 575
BstKTI GATC 6 cut(s) 136, 638, 923, 1671, 1712, 2234
BstMAI GTCTC 6 cut(s) 834, 864, 870, 1103, 1109, 1443
BstMBI GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
BstMWI GCNNNNNNNGC 7 cut(s) 243, 496, 680, 734, 755, 764, 1148
BstNI CCWGG 4 cut(s) 297, 352, 1560, 2252
BstNSI RCATGY 4 cut(s) 490, 783, 1250, 1649
BstSCI CCNGG 6 cut(s) 295, 350, 982, 1558, 1840, 2250
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 2 cut(s) 183, 751
BstSNI TACGTA 1 cut(s) 1876
BstX2I RGATCY 2 cut(s) 133, 1709
BstXI CCANNNNNNTGG 3 cut(s) 507, 1765, 2251
BstYI RGATCY 2 cut(s) 133, 1709
Bsu15I ATCGAT 1 cut(s) 2119
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1845, 2142
BsuTUI ATCGAT 1 cut(s) 2119
BtgI CCRYGG 2 cut(s) 1007, 1632
BtrI CACGTC 1 cut(s) 1858
BtsCI GGATG 5 cut(s) 89, 1017, 1320, 1362, 1758
BtsI GCAGTG 2 cut(s) 540, 774
BtsIMutI CAGTG 9 cut(s) 183, 540, 774, 907, 1017, 2001, 2040, 2104, 2157
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 6 cut(s) 171, 335, 501, 1452, 1639, 1953
CaiI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 1 cut(s) 575
Cfr13I GGNCC 6 cut(s) 364, 872, 1511, 1584, 1692, 1768
ClaI ATCGAT 1 cut(s) 2119
CseI GACGC 1 cut(s) 1956
Csp6I GTAC 3 cut(s) 772, 1626, 1941
CviQI GTAC 3 cut(s) 772, 1626, 1941
DdeI CTNAG 9 cut(s) 204, 603, 885, 1100, 1123, 1251, 1807, 2278, 2288
DpnI GATC 6 cut(s) 135, 637, 922, 1670, 1711, 2233
DpnII GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
DraIII CACNNNGTG 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 2140
Eam1104I CTCTTC 3 cut(s) 603, 1112, 1740
EarI CTCTTC 3 cut(s) 603, 1112, 1740
EciI GGCGGA 1 cut(s) 1129
Eco105I TACGTA 1 cut(s) 1876
Eco130I CCWWGG 5 cut(s) 93, 641, 1007, 1215, 1632
Eco32I GATATC 2 cut(s) 853, 1060
Eco47I GGWCC 4 cut(s) 872, 1584, 1692, 1768
Eco57I CTGAAG 4 cut(s) 223, 1167, 1353, 1794
Eco81I CCTNAGG 1 cut(s) 885
EcoO109I RGGNCCY 2 cut(s) 872, 1511
EcoRI GAATTC 1 cut(s) 1880
EcoRII CCWGG 4 cut(s) 295, 350, 1558, 2250
EcoRV GATATC 2 cut(s) 853, 1060
EcoT14I CCWWGG 5 cut(s) 93, 641, 1007, 1215, 1632
EcoT22I ATGCAT 1 cut(s) 2203
ErhI CCWWGG 5 cut(s) 93, 641, 1007, 1215, 1632
Esp3I CGTCTC 1 cut(s) 834
FaqI GGGAC 6 cut(s) 659, 1337, 1482, 1570, 1678, 2096
FauI CCCGC 1 cut(s) 1459
FbaI TGATCA 1 cut(s) 1668
FblI GTMKAC 1 cut(s) 1243
FokI GGATG 5 cut(s) 76, 1024, 1327, 1349, 1745
GlaI GCGC 1 cut(s) 574
GsaI CCCAGC 2 cut(s) 1592, 1700
GsuI CTGGAG 1 cut(s) 750
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 7 cut(s) 366, 457, 821, 1220, 1512, 1845, 2142
HapII CCGG 5 cut(s) 494, 654, 984, 1529, 1842
HgaI GACGC 1 cut(s) 1956
HhaI GCGC 1 cut(s) 575
Hin6I GCGC 1 cut(s) 573
HinP1I GCGC 1 cut(s) 573
HincII GTYRAC 4 cut(s) 261, 615, 808, 1244
HindII GTYRAC 4 cut(s) 261, 615, 808, 1244
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 7 cut(s) 461, 611, 1265, 1656, 1830, 2094, 2116
HpaI GTTAAC 1 cut(s) 261
HpaII CCGG 5 cut(s) 494, 654, 984, 1529, 1842
HphI GGTGA 6 cut(s) 140, 386, 630, 902, 1103, 2076
Hpy188I TCNGA 9 cut(s) 242, 468, 635, 839, 920, 1057, 1147, 1972, 2261
HpyAV CCTTC 8 cut(s) 505, 968, 1222, 1377, 1769, 1776, 1903, 2185
HpyCH4III ACNGT 8 cut(s) 224, 1021, 1190, 1940, 1996, 2035, 2154, 2161
HpyCH4IV ACGT 4 cut(s) 804, 1857, 1875, 1943
HpyF10VI GCNNNNNNNGC 7 cut(s) 243, 496, 680, 734, 755, 764, 1148
HpyF3I CTNAG 9 cut(s) 204, 603, 885, 1100, 1123, 1251, 1807, 2278, 2288
HpySE526I ACGT 4 cut(s) 804, 1857, 1875, 1943
HspAI GCGC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 1668
KspAI GTTAAC 1 cut(s) 261
Kzo9I GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
LmnI GCTCC 4 cut(s) 656, 886, 1561, 2185
LweI GCATC 9 cut(s) 218, 282, 469, 820, 868, 895, 1002, 1405, 1586
MaeII ACGT 4 cut(s) 804, 1857, 1875, 1943
MalI GATC 6 cut(s) 135, 637, 922, 1670, 1711, 2233
MboI GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
MflI RGATCY 2 cut(s) 133, 1709
MhlI GDGCHC 2 cut(s) 183, 751
MlsI TGGCCA 1 cut(s) 2142
MluNI TGGCCA 1 cut(s) 2142
MlyI GAGTC 3 cut(s) 620, 1274, 1839
MmeI TCCRAC 2 cut(s) 898, 2071
Mox20I TGGCCA 1 cut(s) 2142
Mph1103I ATGCAT 1 cut(s) 2203
MroXI GAANNNNTTC 1 cut(s) 1135
MscI TGGCCA 1 cut(s) 2142
MseI TTAA 4 cut(s) 260, 420, 1416, 2123
MslI CAYNNNNRTG 1 cut(s) 1763
Msp20I TGGCCA 1 cut(s) 2142
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 5 cut(s) 494, 654, 984, 1529, 1842
MspR9I CCNGG 6 cut(s) 297, 352, 984, 1560, 1842, 2252
Mva1269I GAATGC 5 cut(s) 36, 275, 1793, 1861, 2201
MvaI CCWGG 4 cut(s) 297, 352, 1560, 2252
MwoI GCNNNNNNNGC 7 cut(s) 243, 496, 680, 734, 755, 764, 1148
NciI CCSGG 2 cut(s) 984, 1842
NcoI CCATGG 2 cut(s) 1007, 1632
NdeII GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
NlaIV GGNNCC 6 cut(s) 135, 498, 1513, 1586, 1694, 1770
NmuCI GTSAC 4 cut(s) 665, 890, 1549, 2035
NsiI ATGCAT 1 cut(s) 2203
NspI RCATGY 4 cut(s) 490, 783, 1250, 1649
NspV TTCGAA 3 cut(s) 323, 1110, 1960
PagI TCATGA 1 cut(s) 1719
PciI ACATGT 1 cut(s) 1645
PctI GAATGC 5 cut(s) 36, 275, 1793, 1861, 2201
PdmI GAANNNNTTC 1 cut(s) 1135
PfeI GAWTC 4 cut(s) 461, 1656, 2094, 2116
PfoI TCCNGGA 1 cut(s) 1558
PleI GAGTC 3 cut(s) 619, 1273, 1838
PpsI GAGTC 3 cut(s) 619, 1273, 1838
Ppu21I YACGTR 2 cut(s) 1876, 1944
PpuMI RGGWCCY 1 cut(s) 872
PscI ACATGT 1 cut(s) 1645
PshBI ATTAAT 1 cut(s) 2123
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 4 cut(s) 295, 350, 1558, 2250
PspFI CCCAGC 2 cut(s) 1588, 1696
PspGI CCWGG 4 cut(s) 295, 350, 1558, 2250
PspN4I GGNNCC 6 cut(s) 135, 498, 1513, 1586, 1694, 1770
PspPI GGNCC 6 cut(s) 364, 872, 1511, 1584, 1692, 1768
PspPPI RGGWCCY 1 cut(s) 872
PstNI CAGNNNCTG 3 cut(s) 1188, 1559, 1970
PsuI RGATCY 2 cut(s) 133, 1709
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 3 cut(s) 773, 1627, 1942
RsaNI GTAC 3 cut(s) 772, 1626, 1941
RseI CAYNNNNRTG 1 cut(s) 1763
SalI GTCGAC 1 cut(s) 1242
SaqAI TTAA 4 cut(s) 260, 420, 1416, 2123
Sau3AI GATC 6 cut(s) 133, 635, 920, 1668, 1709, 2231
Sau96I GGNCC 6 cut(s) 364, 872, 1511, 1584, 1692, 1768
SchI GAGTC 3 cut(s) 620, 1274, 1839
ScrFI CCNGG 6 cut(s) 297, 352, 984, 1560, 1842, 2252
SduI GDGCHC 2 cut(s) 183, 751
SfaNI GCATC 9 cut(s) 218, 282, 469, 820, 868, 895, 1002, 1405, 1586
SfcI CTRYAG 1 cut(s) 678
SfuI TTCGAA 3 cut(s) 323, 1110, 1960
SinI GGWCC 4 cut(s) 872, 1584, 1692, 1768
SmiMI CAYNNNNRTG 1 cut(s) 1763
SnaBI TACGTA 1 cut(s) 1876
SsiI CCGC 4 cut(s) 818, 1140, 1452, 1662
StyD4I CCNGG 6 cut(s) 295, 350, 982, 1558, 1840, 2250
StyI CCWWGG 5 cut(s) 93, 641, 1007, 1215, 1632
TaaI ACNGT 8 cut(s) 224, 1021, 1190, 1940, 1996, 2035, 2154, 2161
TaiI ACGT 4 cut(s) 807, 1860, 1878, 1946
TaqI TCGA 9 cut(s) 323, 577, 1110, 1243, 1441, 1797, 1960, 2119, 2189
TatI WGTACW 1 cut(s) 771
TauI GCSGC 2 cut(s) 821, 1664
TfiI GAWTC 4 cut(s) 461, 1656, 2094, 2116
Tru1I TTAA 4 cut(s) 260, 420, 1416, 2123
Tru9I TTAA 4 cut(s) 260, 420, 1416, 2123
TscAI CASTG 9 cut(s) 190, 547, 774, 907, 1024, 2001, 2040, 2104, 2164
TseFI GTSAC 4 cut(s) 665, 890, 1549, 2035
Tsp45I GTSAC 4 cut(s) 665, 890, 1549, 2035
TspGWI ACGGA 2 cut(s) 1161, 1441
TspRI CASTG 9 cut(s) 190, 547, 774, 907, 1024, 2001, 2040, 2104, 2164
VneI GTGCAC 2 cut(s) 179, 747
VpaK11BI GGWCC 4 cut(s) 872, 1584, 1692, 1768
VspI ATTAAT 1 cut(s) 2123
XapI RAATTY 7 cut(s) 282, 450, 536, 945, 997, 1443, 1880
XceI RCATGY 4 cut(s) 490, 783, 1250, 1649
XmiI GTMKAC 1 cut(s) 1243
XmnI GAANNNNTTC 1 cut(s) 1135
Zsp2I ATGCAT 1 cut(s) 2203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.