Rroxscaffold_2G00145450

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016727
Physical Location & Seq
Forward (+)
83378778 .. 83383685
4908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145450.1

Sequence Viewer

Length: 2088 bp
ATGAATGCGGTAGTGATTAAGAAGTTGGTAATGGGTTATGATGATGACGAGGTGTATGTGGTTTATATGGGAAGCAAAAATGGTGAAGACCCAGATGAGATTCTGGCGCAAAACCAGCAAATACTTGCTTCTGTTCACACAGGAAGCATAGAGGAGGCCCAAGCATCTCACATTCATAGTTACCGACATGGTTTTAAAGGCTTTGCTGCCAGGTTGACTGATCAGCAAGCTTTCCAAATTTCAAGAATTTGGCCTGAATCTCCAAGTTTTAATGATGACAACATGCCTCCAGTGCCAGCTAGATGGAAGGGGGTTTGTCAATCAGGAGAGGCATTCAATGCTTCAACTTGCAACAGAAAAGTGATTGGGGCTAGATACTATAAGTCCGGTTATGAAGCCGAGGAAGATTCAGCGGACACAGTGGCATTCAGTTCTCCAAGGGACAGTGCCGGTCATGGCAGCCACACAGCCTCTATAGCTGCTGGTCGTTATGTTTCAAACATGACTTATAAGGGATTGGCATCTGGAGGAGCTAGAGGAGGTGCACCAATGGCCAGAATTGCAGTATATAAAACTTGTTGGGACACTGGTTGTTATGACGTAGACTTACTAGCAGCTTTTGATGATGCAATTAGAGATGGGGTTCACATTCTGTCTCTGTCTCTCGGGCCTGATGCTCCTCAGGGAGACTATTTCACTGATGCCATTTCTGTGGGGTCATTCCATGCTGCTAGGCATGGAATTCTAGTGGTTGCTTCTGCTGGAAATGAAGGATACCCGGGTTCTGCAACAAATCTCGCTCCATGGATGATTACTGTTGCTGCAAGTTCAACGGATAGGGATTTCACTTCTGATATCGTTCTTGGAAATGGTGTTAATGTCACGGGTGAAAGCCTCAGTGTATTTGGAATGAAAGCCTCAGCAAGTATCATTTCCGCCTCTGAAGCCTTTGCAGGGTACTTCACTCCATATCAATCCAGTTATTGTTTAGAAAGTTCCTTGAACAAGACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGTCATGCTGAGAGTTCAACAGAGTCGAAGCTTGCAAAAAGTGTAGTAGTTAAAGAAGCTGGTGGTGTTGGGATGGTTCTTATCGATGAGGCAGATATGGATGTTGCCGTCCCTTTTGTTATCCCTTCAGCAATTATTAGACGGAGGATAGGAAAACATCTTCTATCTTACATTAATCGCACACGCCAACCAATGTCGAGAATTCTCCCTGCAAAGACTACACTGGGATTGAAACCTGCACCTCATGTTACAGCATTTTCTGCAAAAGGTCCCAATGCTTTGACACCAGAAATTTTGAAGCCTGATATCACAGCTCCTGGACTGAACATACTTGCATCATGGTCCCCAGCAGTAGCTGGTAAGCAGTTCAACATTCTTTCTGGTACATCCATGGCTTGCCCGCATGTAACAGGAATTGCCACCTTGATAAAAGCCATTCATCCTTCATGGTCCCCAGCAGCTATTAGATCGGCAATCATGACTACTGCTACTCTTCTGGACAAGATGCACAAGCCGATTACAGTGGACCCTGAAGGCAAAAAGGGTAATGCATTTGATTATGGCTCAGGCTTCCTGAACCCAACAAGAGCACTTGATCCAGGTCTTGTCTTTGACGCACAGCCAGCAGATTATGTATCATTCCTTTGTTCAGTTGGATATGATGAGAAGTCATTGCATCAAATCACACTAGACAACAGTACATGTGCTCAGTCATTCAGAACAGCATCTGACCTTAACTATCCATCTATCACTGTACCAAATCTCGAAGGGAACATCTCAGTAACTAGAACAGTGACCAACGTCGGGAAGCCAAAGAATATCTATAAAGCCGCTGTGTCCTCACCCAAAGGAATCAATGTTACTGTTATTCCAAATCAACTAGTCTTCACGAGCCTCGGTCAGAAGCTAAGCTTCACTGTGAATTTCAAGGTGGTTGCTCCATCAAAGGGATATGCATTTGGGTTCTTGTCATGGATGAGTGGACGATCGAGGGTTACCAGTCCTCTCGTTGTCCAAGTTGCGCATTCAAACTCAGGGCTGATGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

695

Amino Acids

74.06

Weight (kDa)

8.52

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 18 - 82 2.9e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 131 - 534 1.9e-42 Subtilase family
fn3_6 PF17766 590 - 684 1.9e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 510
Acc16I TGCGCA 1 cut(s) 2061
Acc36I ACCTGC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 603
AciI CCGC 5 cut(s) 8, 413, 936, 1439, 1869
AclWI GGATC 1 cut(s) 1628
AcoI YGGCCR 1 cut(s) 552
AcsI RAATTY 6 cut(s) 237, 246, 741, 1239, 1329, 1960
AcuI CTGAAG 3 cut(s) 963, 1149, 1590
AfaI GTAC 4 cut(s) 959, 1423, 1738, 1794
AfiI CCNNNNNNNGG 3 cut(s) 954, 1842, 1985
AflIII ACRYGT 1 cut(s) 1739
AhlI ACTAGT 1 cut(s) 1918
AjnI CCWGG 3 cut(s) 209, 1354, 1636
Alw21I GWGCWC 3 cut(s) 547, 1630, 1747
Alw26I GTCTC 3 cut(s) 660, 666, 681
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 1 cut(s) 1628
AlwNI CAGNNNCTG 3 cut(s) 1355, 1394, 1766
Ama87I CYCGRG 2 cut(s) 665, 778
AoxI GGCC 5 cut(s) 156, 251, 552, 668, 1014
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 7 cut(s) 206, 459, 479, 614, 728, 821, 1496
ApoI RAATTY 6 cut(s) 237, 246, 741, 1239, 1329, 1960
AseI ATTAAT 1 cut(s) 1212
AspLEI GCGC 2 cut(s) 109, 2062
AspS9I GGNCC 6 cut(s) 157, 668, 1307, 1380, 1488, 1564
AsuC2I CCSGG 2 cut(s) 779, 780
AsuHPI GGTGA 3 cut(s) 95, 899, 1872
AvaI CYCGRG 2 cut(s) 665, 778
AvaII GGWCC 4 cut(s) 1307, 1380, 1488, 1564
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 547
BaeI ACNNNNGTAYC 2 cut(s) 766, 799
BalI TGGCCA 1 cut(s) 554
BauI CACGAG 1 cut(s) 1927
BbsI GAAGAC 2 cut(s) 93, 1915
Bbv12I GWGCWC 3 cut(s) 547, 1630, 1747
BbvCI CCTCAGC 1 cut(s) 919
BbvI GCAGC 7 cut(s) 193, 466, 471, 626, 715, 808, 1508
BccI CCATC 5 cut(s) 297, 632, 1105, 1789, 1987
BceAI ACGGC 1 cut(s) 1130
BciT130I CCWGG 3 cut(s) 211, 1356, 1638
BciVI GTATCC 1 cut(s) 767
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 779, 780
BcoDI GTCTC 3 cut(s) 660, 666, 681
BcuI ACTAGT 1 cut(s) 1918
BfaI CTAG 9 cut(s) 300, 372, 534, 611, 732, 746, 1727, 1824, 1919
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 1282
BfuI GTATCC 1 cut(s) 767
BisI GCNGC 8 cut(s) 207, 460, 480, 615, 729, 822, 1497, 1869
BlpI GCTNAGC 1 cut(s) 1946
BlsI GCNGC 8 cut(s) 208, 461, 481, 616, 730, 823, 1498, 1870
Bme1390I CCNGG 5 cut(s) 211, 779, 780, 1356, 1638
Bme18I GGWCC 4 cut(s) 1307, 1380, 1488, 1564
BmeT110I CYCGRG 2 cut(s) 665, 778
BmgT120I GGNCC 6 cut(s) 157, 668, 1307, 1380, 1488, 1564
BmiI GGNNCC 4 cut(s) 1309, 1382, 1490, 1566
BmrFI CCNGG 5 cut(s) 211, 779, 780, 1356, 1638
BmrI ACTGGG 1 cut(s) 1271
BmsI GCATC 9 cut(s) 173, 530, 616, 664, 691, 1382, 1533, 1723, 1772
BmuI ACTGGG 1 cut(s) 1271
BoxI GACNNNNGTC 1 cut(s) 1838
BpiI GAAGAC 2 cut(s) 93, 1915
BpmI CTGGAG 2 cut(s) 273, 546
Bpu10I CCTNAGC 2 cut(s) 919, 1603
Bpu1102I GCTNAGC 1 cut(s) 1946
BpuMI CCSGG 2 cut(s) 779, 780
Bsa29I ATCGAT 1 cut(s) 1122
BsaBI GATNNNNATC 1 cut(s) 520
BsaJI CCNNGG 7 cut(s) 399, 437, 778, 803, 1011, 1428, 1933
BsaWI WCCGGW 1 cut(s) 386
Bsc4I CCNNNNNNNGG 3 cut(s) 954, 1842, 1985
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 5 cut(s) 290, 592, 978, 1266, 2037
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 1709
Bse8I GATNNNNATC 1 cut(s) 520
BseBI CCWGG 3 cut(s) 211, 1356, 1638
BseCI ATCGAT 1 cut(s) 1122
BseDI CCNNGG 7 cut(s) 399, 437, 778, 803, 1011, 1428, 1933
BseGI GGATG 6 cut(s) 813, 1116, 1144, 1424, 1477, 2019
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 3 cut(s) 954, 1842, 1985
BseMI GCAATG 1 cut(s) 1709
BseMII CTCAG 8 cut(s) 695, 910, 933, 1038, 1617, 1760, 1830, 2085
BseNI ACTGG 5 cut(s) 290, 592, 978, 1266, 2037
BseRI GAGGAG 4 cut(s) 167, 543, 552, 669
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 7 cut(s) 193, 466, 471, 626, 715, 808, 1508
BseYI CCCAGC 2 cut(s) 1384, 1492
BsgI GTGCAG 1 cut(s) 1260
Bsh1285I CGRYCG 1 cut(s) 2027
BshFI GGCC 5 cut(s) 158, 253, 554, 670, 1016
BshVI ATCGAT 1 cut(s) 1122
BsiEI CGRYCG 1 cut(s) 2027
BsiHKAI GWGCWC 3 cut(s) 547, 1630, 1747
BsiHKCI CYCGRG 2 cut(s) 665, 778
BsiSI CCGG 3 cut(s) 387, 450, 779
BslFI GGGAC 6 cut(s) 455, 596, 1133, 1293, 1366, 1474
BslI CCNNNNNNNGG 3 cut(s) 954, 1842, 1985
BsmAI GTCTC 3 cut(s) 660, 666, 681
BsmFI GGGAC 6 cut(s) 455, 596, 1133, 1293, 1366, 1474
BsmI GAATGC 4 cut(s) 10, 332, 425, 2062
BsnI GGCC 5 cut(s) 158, 253, 554, 670, 1016
BsoBI CYCGRG 2 cut(s) 665, 778
Bsp1286I GDGCHC 3 cut(s) 547, 1630, 1747
Bsp143I GATC 4 cut(s) 220, 1505, 1633, 2024
Bsp1720I GCTNAGC 1 cut(s) 1946
Bsp19I CCATGG 2 cut(s) 803, 1428
BspACI CCGC 5 cut(s) 8, 413, 936, 1439, 1869
BspANI GGCC 5 cut(s) 158, 253, 554, 670, 1016
BspCNI CTCAG 8 cut(s) 694, 909, 932, 1039, 1616, 1759, 1829, 2084
BspDI ATCGAT 1 cut(s) 1122
BspHI TCATGA 1 cut(s) 1515
BspLI GGNNCC 4 cut(s) 1309, 1382, 1490, 1566
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 1 cut(s) 1628
BsrDI GCAATG 1 cut(s) 1709
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 5 cut(s) 290, 592, 978, 1266, 2037
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 7 cut(s) 399, 437, 778, 803, 1011, 1428, 1933
BssMI GATC 4 cut(s) 220, 1505, 1633, 2024
BssSI CACGAG 1 cut(s) 1927
BssT1I CCWWGG 4 cut(s) 437, 803, 1011, 1428
Bst2BI CACGAG 1 cut(s) 1927
Bst2UI CCWGG 3 cut(s) 211, 1356, 1638
Bst4CI ACNGT 9 cut(s) 421, 446, 817, 1561, 1736, 1792, 1831, 1903, 1957
Bst6I CTCTTC 1 cut(s) 1536
BstAPI GCANNNNNTGC 2 cut(s) 338, 1298
BstC8I GCNNGC 6 cut(s) 228, 297, 1071, 1435, 1439, 1662
BstDEI CTNAG 9 cut(s) 681, 896, 919, 1047, 1603, 1746, 1816, 1946, 2071
BstDSI CCRYGG 2 cut(s) 803, 1428
BstEII GGTNACC 1 cut(s) 2032
BstF5I GGATG 6 cut(s) 813, 1116, 1144, 1424, 1477, 2019
BstHHI GCGC 2 cut(s) 109, 2062
BstKTI GATC 4 cut(s) 223, 1508, 1636, 2027
BstMAI GTCTC 3 cut(s) 660, 666, 681
BstMBI GATC 4 cut(s) 220, 1505, 1633, 2024
BstMCI CGRYCG 1 cut(s) 2027
BstMWI GCNNNNNNNGC 9 cut(s) 115, 292, 338, 476, 551, 560, 944, 1298, 1661
BstNI CCWGG 3 cut(s) 211, 1356, 1638
BstNSI RCATGY 3 cut(s) 286, 1445, 1743
BstPAI GACNNNNGTC 1 cut(s) 1838
BstPI GGTNACC 1 cut(s) 2032
BstSCI CCNGG 5 cut(s) 209, 777, 778, 1354, 1636
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 7 cut(s) 193, 466, 471, 626, 715, 808, 1508
BstV2I GAAGAC 2 cut(s) 93, 1915
BstXI CCANNNNNNTGG 2 cut(s) 303, 712
Bsu15I ATCGAT 1 cut(s) 1122
Bsu36I CCTNAGG 1 cut(s) 681
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 5 cut(s) 158, 253, 554, 670, 1016
BsuTUI ATCGAT 1 cut(s) 1122
BtgI CCRYGG 2 cut(s) 803, 1428
BtsCI GGATG 6 cut(s) 813, 1116, 1144, 1424, 1477, 2019
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 6 cut(s) 228, 297, 1071, 1435, 1439, 1662
CaiI CAGNNNCTG 3 cut(s) 1355, 1394, 1766
CciI TCATGA 1 cut(s) 1515
CfoI GCGC 2 cut(s) 109, 2062
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 6 cut(s) 157, 668, 1307, 1380, 1488, 1564
Cfr9I CCCGGG 1 cut(s) 778
ClaI ATCGAT 1 cut(s) 1122
CseI GACGC 1 cut(s) 1661
Csp6I GTAC 4 cut(s) 958, 1422, 1737, 1793
CviQI GTAC 4 cut(s) 958, 1422, 1737, 1793
DdeI CTNAG 9 cut(s) 681, 896, 919, 1047, 1603, 1746, 1816, 1946, 2071
DpnI GATC 4 cut(s) 222, 1507, 1635, 2026
DpnII GATC 4 cut(s) 220, 1505, 1633, 2024
DraI TTTAAA 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 552
Eam1104I CTCTTC 1 cut(s) 1536
EarI CTCTTC 1 cut(s) 1536
EciI GGCGGA 1 cut(s) 925
Eco130I CCWWGG 4 cut(s) 437, 803, 1011, 1428
Eco32I GATATC 2 cut(s) 856, 1345
Eco47I GGWCC 4 cut(s) 1307, 1380, 1488, 1564
Eco57I CTGAAG 3 cut(s) 963, 1149, 1590
Eco81I CCTNAGG 1 cut(s) 681
Eco88I CYCGRG 2 cut(s) 665, 778
Eco91I GGTNACC 1 cut(s) 2032
EcoO109I RGGNCCY 1 cut(s) 1307
EcoO65I GGTNACC 1 cut(s) 2032
EcoRI GAATTC 2 cut(s) 741, 1239
EcoRII CCWGG 3 cut(s) 209, 1354, 1636
EcoRV GATATC 2 cut(s) 856, 1345
EcoT14I CCWWGG 4 cut(s) 437, 803, 1011, 1428
EcoT22I ATGCAT 2 cut(s) 1591, 1996
ErhI CCWWGG 4 cut(s) 437, 803, 1011, 1428
FalI AAGNNNNNCTT 2 cut(s) 1934, 1966
FaqI GGGAC 6 cut(s) 455, 596, 1133, 1293, 1366, 1474
FauI CCCGC 1 cut(s) 1446
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 8 cut(s) 207, 460, 480, 615, 729, 822, 1497, 1869
FokI GGATG 6 cut(s) 820, 1123, 1151, 1411, 1464, 2026
Fsp4HI GCNGC 8 cut(s) 207, 460, 480, 615, 729, 822, 1497, 1869
FspBI CTAG 9 cut(s) 300, 372, 534, 611, 732, 746, 1727, 1824, 1919
FspI TGCGCA 1 cut(s) 2061
GlaI GCGC 2 cut(s) 108, 2061
GluI GCNGC 8 cut(s) 207, 460, 480, 615, 729, 822, 1497, 1869
GsaI CCCAGC 2 cut(s) 1388, 1496
GsuI CTGGAG 2 cut(s) 273, 546
HaeIII GGCC 5 cut(s) 158, 253, 554, 670, 1016
HapII CCGG 3 cut(s) 387, 450, 779
HgaI GACGC 1 cut(s) 1661
HhaI GCGC 2 cut(s) 109, 2062
Hin6I GCGC 2 cut(s) 107, 2060
HinP1I GCGC 2 cut(s) 107, 2060
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 3 cut(s) 228, 1067, 1948
HinfI GANTC 5 cut(s) 100, 257, 407, 1061, 1890
HpaII CCGG 3 cut(s) 387, 450, 779
HphI GGTGA 3 cut(s) 95, 899, 1872
Hpy166II GTNNAC 7 cut(s) 136, 216, 545, 604, 646, 1564, 2021
Hpy188I TCNGA 5 cut(s) 853, 943, 1757, 1768, 1941
Hpy8I GTNNAC 7 cut(s) 136, 216, 545, 604, 646, 1564, 2021
Hpy99I CGWCG 1 cut(s) 1844
HpyAV CCTTC 7 cut(s) 301, 764, 1018, 1173, 1491, 1565, 1799
HpyCH4III ACNGT 9 cut(s) 421, 446, 817, 1561, 1736, 1792, 1831, 1903, 1957
HpyCH4IV ACGT 2 cut(s) 600, 1839
HpyF10VI GCNNNNNNNGC 9 cut(s) 115, 292, 338, 476, 551, 560, 944, 1298, 1661
HpyF3I CTNAG 9 cut(s) 681, 896, 919, 1047, 1603, 1746, 1816, 1946, 2071
HpySE526I ACGT 2 cut(s) 600, 1839
HspAI GCGC 2 cut(s) 107, 2060
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 4 cut(s) 220, 1505, 1633, 2024
LmnI GCTCC 5 cut(s) 530, 682, 805, 1357, 1981
Lsp1109I GCAGC 7 cut(s) 193, 466, 471, 626, 715, 808, 1508
LweI GCATC 9 cut(s) 173, 530, 616, 664, 691, 1382, 1533, 1723, 1772
MaeI CTAG 9 cut(s) 300, 372, 534, 611, 732, 746, 1727, 1824, 1919
MaeII ACGT 2 cut(s) 600, 1839
MaeIII GTNAC 8 cut(s) 179, 880, 1285, 1444, 1819, 1831, 1897, 2032
MalI GATC 4 cut(s) 222, 1507, 1635, 2026
MboI GATC 4 cut(s) 220, 1505, 1633, 2024
MboII GAAGA 5 cut(s) 98, 416, 1190, 1523, 1915
MhlI GDGCHC 3 cut(s) 547, 1630, 1747
MlsI TGGCCA 1 cut(s) 554
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 1 cut(s) 1672
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 2 cut(s) 1591, 1996
MscI TGGCCA 1 cut(s) 554
MseI TTAA 7 cut(s) 18, 195, 270, 876, 1089, 1212, 1773
MslI CAYNNNNRTG 1 cut(s) 710
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 2 cut(s) 413, 1871
MspI CCGG 3 cut(s) 387, 450, 779
MspR9I CCNGG 5 cut(s) 211, 779, 780, 1356, 1638
Mva1269I GAATGC 4 cut(s) 10, 332, 425, 2062
MvaI CCWGG 3 cut(s) 211, 1356, 1638
MwoI GCNNNNNNNGC 9 cut(s) 115, 292, 338, 476, 551, 560, 944, 1298, 1661
NciI CCSGG 2 cut(s) 779, 780
NcoI CCATGG 2 cut(s) 803, 1428
NdeII GATC 4 cut(s) 220, 1505, 1633, 2024
NlaIV GGNNCC 4 cut(s) 1309, 1382, 1490, 1566
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 880, 1831
NsbI TGCGCA 1 cut(s) 2061
NsiI ATGCAT 2 cut(s) 1591, 1996
NspI RCATGY 3 cut(s) 286, 1445, 1743
PagI TCATGA 1 cut(s) 1515
PciI ACATGT 1 cut(s) 1739
PctI GAATGC 4 cut(s) 10, 332, 425, 2062
PfeI GAWTC 4 cut(s) 100, 257, 407, 1890
PfoI TCCNGGA 1 cut(s) 1354
PkrI GCNGC 8 cut(s) 208, 461, 481, 616, 730, 823, 1498, 1870
Ple19I CGATCG 1 cut(s) 2027
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
PpuMI RGGWCCY 1 cut(s) 1307
PscI ACATGT 1 cut(s) 1739
PshAI GACNNNNGTC 1 cut(s) 1838
PshBI ATTAAT 1 cut(s) 1212
PsiI TTATAA 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 1307
Psp6I CCWGG 3 cut(s) 209, 1354, 1636
PspEI GGTNACC 1 cut(s) 2032
PspFI CCCAGC 2 cut(s) 1384, 1492
PspGI CCWGG 3 cut(s) 209, 1354, 1636
PspN4I GGNNCC 4 cut(s) 1309, 1382, 1490, 1566
PspPI GGNCC 6 cut(s) 157, 668, 1307, 1380, 1488, 1564
PspPPI RGGWCCY 1 cut(s) 1307
PstNI CAGNNNCTG 3 cut(s) 1355, 1394, 1766
PvuI CGATCG 1 cut(s) 2027
RsaI GTAC 4 cut(s) 959, 1423, 1738, 1794
RsaNI GTAC 4 cut(s) 958, 1422, 1737, 1793
RseI CAYNNNNRTG 1 cut(s) 710
SaqAI TTAA 7 cut(s) 18, 195, 270, 876, 1089, 1212, 1773
SatI GCNGC 8 cut(s) 207, 460, 480, 615, 729, 822, 1497, 1869
Sau3AI GATC 4 cut(s) 220, 1505, 1633, 2024
Sau96I GGNCC 6 cut(s) 157, 668, 1307, 1380, 1488, 1564
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 5 cut(s) 211, 779, 780, 1356, 1638
SduI GDGCHC 3 cut(s) 547, 1630, 1747
SfaNI GCATC 9 cut(s) 173, 530, 616, 664, 691, 1382, 1533, 1723, 1772
SfcI CTRYAG 1 cut(s) 474
SinI GGWCC 4 cut(s) 1307, 1380, 1488, 1564
SmaI CCCGGG 1 cut(s) 780
SmiMI CAYNNNNRTG 1 cut(s) 710
SpeI ACTAGT 1 cut(s) 1918
SsiI CCGC 5 cut(s) 8, 413, 936, 1439, 1869
SspMI CTAG 9 cut(s) 300, 372, 534, 611, 732, 746, 1727, 1824, 1919
StyD4I CCNGG 5 cut(s) 209, 777, 778, 1354, 1636
StyI CCWWGG 4 cut(s) 437, 803, 1011, 1428
TaaI ACNGT 9 cut(s) 421, 446, 817, 1561, 1736, 1792, 1831, 1903, 1957
TaiI ACGT 2 cut(s) 603, 1842
TaqI TCGA 5 cut(s) 1064, 1122, 1235, 1803, 2027
TaqII GACCGA 1 cut(s) 1925
TatI WGTACW 1 cut(s) 1736
TauI GCSGC 1 cut(s) 1871
TfiI GAWTC 4 cut(s) 100, 257, 407, 1890
Tru1I TTAA 7 cut(s) 18, 195, 270, 876, 1089, 1212, 1773
Tru9I TTAA 7 cut(s) 18, 195, 270, 876, 1089, 1212, 1773
TseFI GTSAC 2 cut(s) 880, 1831
TseI GCWGC 7 cut(s) 206, 459, 479, 614, 728, 821, 1496
Tsp45I GTSAC 2 cut(s) 880, 1831
TspDTI ATGAA 7 cut(s) 17, 164, 408, 783, 926, 1466, 1473
TspGWI ACGGA 2 cut(s) 848, 1195
TspMI CCCGGG 1 cut(s) 778
VneI GTGCAC 1 cut(s) 543
VpaK11BI GGWCC 4 cut(s) 1307, 1380, 1488, 1564
VspI ATTAAT 1 cut(s) 1212
XapI RAATTY 6 cut(s) 237, 246, 741, 1239, 1329, 1960
XceI RCATGY 3 cut(s) 286, 1445, 1743
XmaI CCCGGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 9 cut(s) 300, 372, 534, 611, 732, 746, 1727, 1824, 1919
Zsp2I ATGCAT 2 cut(s) 1591, 1996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.