MD02G1114000.v1.1

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011468
Physical Location & Seq
Forward (+)
9280796 .. 9285336
4541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1114000.v1.1.491

Sequence Viewer

Length: 2655 bp
ATGTTCTTGTCCATCCAATCTCCTCCGATGGATCGCCGCAATTCGATTCCGATATTTATCCTAATCGTATCACTGATCGCCTGTTTTCTCATCCGAAACGCCGACGTTTCGGATCTCATCGACTTCCAGATTATCGGTCCGCCTCGGAGCTATGACGGCGCCGTTTTGAACTCGGTCAGCAGCAGCCAGTTGGAGCTTCCGGTGTTTTACCAGACTAGAGACGGCGACGTTTTGAACTGGGAGAGCGGGGAGAGCAGGGCATTGCAGCCGTTGGGGTCGCAGCAGAAGGAGCTCGAAGTCGTCTCTCGCTCTCCAAGCCGAAGACTTCACTCCCATACGGAGGATGAGCCTAGAACTGCATTAGTTGCTCTACTGGATGGAACAATACAATTAGTGGATAGAAATTTGATGGGAGTAAGATGGGCGTTTGATTCGGGGCCGCCAATTTACAGTTCCTATCAGGCTCAAAGCAGTAGTGGGTACTTCATATATTGTGGTGATGACTGGGACTTGTACGAGCACAGCGATCATTTTGGTCGACGGAAAATTCCATCGACTATTGCTGAGTATGTGGATAAAAAAACGCCTAAGGTGGAGGAGGATGGATCAGTTACACTTGGACATAAGAAGGTCACTTTCTTTGAGGTTGGTCTTTTTACCGGAAAGGTATACCGCTCATACACTCTATCCGAAGATCATGTTAGAGATCGAAAGAAGTTTGTCAAGCCCAGTTTGATAAATGGAAATGCTGGTGAGCCGCGAATAGTATTCTTCAGAATAGATTATTACTTGGTGTCTTACCATCCGTATTCAGAGGAAGTATCTTGGAATATGACAATTGCTGAAATCAGGGCTGCTATCCCTTGCAAAGATATTGAGAATCTGATTCGTGGTTCTAAAACTGGCAGTCAGATTGCATTACCGTTTGACTGTCAGTCAAAAGCACTTGTTAAGCGCGAGCGAGATCATGGATCATTAGAGCATCAACGGGCAGAAACTGTGGTAAAAAAGTCTGCTTCAGATCTGGTTGTTCCTTTGAACAAAAAAGATCTGGTTGTTCCTTCAAAGCCTGAGGTTCACAATATGTTTCTTCATTGGTCAAAAGCATTTGCTCTCGCGTTTTTGTTTGTAATCCTCATGGGCTTTGTTGTTTACCACTTTGTTTTTCTGGTTAATGGTCAAAAGCCTGATCCGAAAGCTTCACCGTCCAAGAGGAAGAAGAGTAGGAAATTGGGAAAAAATGGAATTGTTGAAAGAAAAGAGGAAAGTGTATTATCTCAAGATGAGGAAGCTTTAACACACACCGAAGGTGATAACAAGATATTGCCATTTCTTAACAAACTCTTTGATGGTGGTACTAATGGTCGCAGGATTGGTAAACTAGTGATTTCAAGTACAGAAATTTCTAAGGGAAGTAATGGTACCATTGTCCTAGAGGGAGTTTATGAAGGTCGACCTGTTGCTGTAAAACGCCTTGTATTAGCCCATCATGACATGGCCTTTAAAGAGATTCAGAATCTTATTGCATCTGACCGACACCCAAACATAGTAAGATGGTATGGAGTGGACTATGATAAAGATTTTGTCTATATCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTGCCCAAATTCCTCTCAAAATCCAGTAGCTGGAGAAGAAGATGCAAAACGAGTTCTGATTGAAAACGAAGTTCGCTTGAAGTCATTGAAGAATATTATGTCAGATGTTAATTTGTGGAAAATAGATGGCTTTCCGTCGCTTCTACTATTGAAATTAATGAGGGATGTGGTTTCTGGTCTTGTGCATTTGCATGAAATGGGGATCATTCATCGCGACTTAAAGCCTCAAAATGTTTTGTTGATTAAGGAAAGAACCTTATGTGCAAAGCTTTCCGACATGGGTATTAGCAAGCGCCTTATCGGGGACATGTCTTCTTTGGGTTGTGGCAGTTCGGGTTGGCAAGCGCCTGAACAGCTTCTCCACGGGCGGCAAACACGTGCAGTGGATATGTTTAGTTTAGGTTGTCTCCTATTTTATTGCATCACAGGTGGTAGACATCCATTCGGTGATCAGCTTGAACGTGATATAAATATTGTAAAGAACCAAATGGATCTCTTCTTGGTAGAGCATATTCCTGAAGCTGTAGATCTTATATCTCGTTTATTAAACCCCGACCCGGAGATAAGGCCAAAGGTATCGGAAGTGCTGCTCCATCCTCTTTTTTGGAACTCCGAGACAAGACTCTCGTTTCTTCGCGACTCCAGTGATAGGGTAGAATTGGAAGATAGAGAGGCTAACTCTGGTGTTTTGAGAGCGCTAGAAAGTATTGCACCCATCGCTTTGGGTGGGAAGTGGGATGAAAAGATGGAGCCTGCATTTCTCACAAACATCGGTAATTACAGGCGGTACAAGTTTGACAGTGTTCGAGACTTATTGCGAGTCATTCGAAACAAATCAAATCACTACAGAGAGCTTCCAAATCAAATTCAGGAACTCGTCGGACCAGTTCCAGAAGGATTCGATTTCTACTTTGCAAGTCGGTTCCCGAGACTTCTGATTGAAGTATACAAAGTTGTGTGCCAAAACTGTAGAGAAGAAGAATGCTTCGGAAAGTATTTCAAAAGCAATGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

885

Amino Acids

100.4

Weight (kDa)

6.94

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 466 - 741 2.6e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 467 - 747 3.4e-35 Protein kinase domain
Ribonuc_2-5A PF06479 753 - 879 1.4e-44 Ribonuclease 2-5A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1421
AccB1I GGYRCC 2 cut(s) 158, 1421
AccB7I CCANNNNNTGG 1 cut(s) 1667
AccBSI CCGCTC 2 cut(s) 246, 675
AccI GTMKAC 5 cut(s) 538, 669, 1453, 2071, 2583
AccII CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
AciI CCGC 8 cut(s) 37, 140, 246, 440, 673, 758, 2005, 2422
AclWI GGATC 7 cut(s) 39, 120, 613, 979, 1184, 1847, 2136
AcsI RAATTY 6 cut(s) 403, 546, 1401, 1635, 1645, 2502
AcuI CTGAAG 3 cut(s) 757, 1002, 2175
AcvI CACGTG 1 cut(s) 2015
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 6 cut(s) 482, 515, 1357, 1396, 1423, 2426
AfeI AGCGCT 1 cut(s) 2334
AfiI CCNNNNNNNGG 5 cut(s) 340, 1667, 1939, 2194, 2286
AflIII ACRYGT 2 cut(s) 1944, 2012
AhdI GACNNNNNGTC 1 cut(s) 934
AhlI ACTAGT 1 cut(s) 1381
AjuI GAANNNNNNNTTGG 2 cut(s) 436, 468
AloI GAACNNNNNNTCC 2 cut(s) 1980, 2012
Alw21I GWGCWC 2 cut(s) 294, 522
Alw26I GTCTC 6 cut(s) 213, 307, 2048, 2246, 2439, 2560
AlwI GGATC 7 cut(s) 39, 120, 613, 979, 1184, 1847, 2136
AlwNI CAGNNNCTG 2 cut(s) 998, 1667
Ama87I CYCGRG 1 cut(s) 2563
Aor51HI AGCGCT 1 cut(s) 2334
AoxI GGCC 3 cut(s) 437, 1497, 2204
ApeKI GCWGC 6 cut(s) 180, 183, 265, 280, 854, 2224
ApoI RAATTY 6 cut(s) 403, 546, 1401, 1635, 1645, 2502
AseI ATTAAT 1 cut(s) 1793
Asp700I GAANNNNTTC 1 cut(s) 1992
Asp718I GGTACC 1 cut(s) 1421
AspLEI GCGC 5 cut(s) 161, 957, 1932, 1984, 2335
AspS9I GGNCC 3 cut(s) 137, 437, 2519
AsuC2I CCSGG 1 cut(s) 2195
AsuHPI GGTGA 5 cut(s) 509, 764, 1194, 1322, 2096
AsuII TTCGAA 1 cut(s) 2464
AvaI CYCGRG 1 cut(s) 2563
AvaII GGWCC 2 cut(s) 137, 2519
AxyI CCTNAGG 2 cut(s) 588, 1071
BaeI ACNNNNGTAYC 2 cut(s) 1413, 1446
BanI GGYRCC 2 cut(s) 158, 1421
BanII GRGCYC 1 cut(s) 294
BarI GAAGNNNNNNTAC 2 cut(s) 1405, 1437
BbrPI CACGTG 1 cut(s) 2015
BbsI GAAGAC 2 cut(s) 328, 1941
Bbv12I GWGCWC 2 cut(s) 294, 522
BbvI GCAGC 6 cut(s) 192, 195, 277, 292, 841, 2211
BceAI ACGGC 4 cut(s) 146, 172, 238, 253
BclI TGATCA 1 cut(s) 2086
BcnI CCSGG 1 cut(s) 2195
BcoDI GTCTC 6 cut(s) 213, 307, 2048, 2246, 2439, 2560
BcuI ACTAGT 1 cut(s) 1381
BfaI CTAG 5 cut(s) 216, 351, 1382, 1433, 2336
BfmI CTRYAG 3 cut(s) 2160, 2482, 2605
BfoI RGCGCY 4 cut(s) 162, 1933, 1985, 2336
BglII AGATCT 3 cut(s) 1021, 1048, 2164
Bme1390I CCNGG 1 cut(s) 2195
Bme18I GGWCC 2 cut(s) 137, 2519
BmeRI GACNNNNNGTC 1 cut(s) 934
BmeT110I CYCGRG 1 cut(s) 2563
BmgT120I GGNCC 3 cut(s) 137, 437, 2519
BmiI GGNNCC 5 cut(s) 160, 438, 1423, 2388, 2561
BmrFI CCNGG 1 cut(s) 2195
BmrI ACTGGG 3 cut(s) 247, 514, 723
BmsI GCATC 4 cut(s) 991, 1535, 1669, 2067
BmuI ACTGGG 3 cut(s) 247, 514, 723
BpiI GAAGAC 2 cut(s) 328, 1941
BplI GAGNNNNNCTC 2 cut(s) 2300, 2332
BpmI CTGGAG 3 cut(s) 1619, 1689, 2263
Bpu14I TTCGAA 1 cut(s) 2464
BpuEI CTTGAG 1 cut(s) 1263
BpuMI CCSGG 1 cut(s) 2195
BsaAI YACGTR 1 cut(s) 2015
BsaBI GATNNNNATC 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 143, 1999
BsaWI WCCGGW 2 cut(s) 199, 659
BsaXI ACNNNNNCTCC 2 cut(s) 1980, 2010
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 1667, 1939, 2194, 2286
Bse1I ACTGG 9 cut(s) 187, 242, 378, 509, 729, 907, 1661, 2280, 2522
Bse21I CCTNAGG 2 cut(s) 588, 1071
Bse3DI GCAATG 2 cut(s) 260, 2650
Bse8I GATNNNNATC 1 cut(s) 56
BseDI CCNNGG 2 cut(s) 143, 1999
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 5 cut(s) 340, 1667, 1939, 2194, 2286
BseMI GCAATG 2 cut(s) 260, 2650
BseMII CTCAG 2 cut(s) 555, 1062
BseNI ACTGG 9 cut(s) 187, 242, 378, 509, 729, 907, 1661, 2280, 2522
BseRI GAGGAG 2 cut(s) 12, 611
BseXI GCAGC 6 cut(s) 192, 195, 277, 292, 841, 2211
BsgI GTGCAG 1 cut(s) 2037
Bsh1236I CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
BshFI GGCC 3 cut(s) 439, 1499, 2206
BshNI GGYRCC 2 cut(s) 158, 1421
BsiHKAI GWGCWC 2 cut(s) 294, 522
BsiHKCI CYCGRG 1 cut(s) 2563
BsiSI CCGG 3 cut(s) 200, 660, 2195
BslFI GGGAC 2 cut(s) 521, 1955
BslI CCNNNNNNNGG 5 cut(s) 340, 1667, 1939, 2194, 2286
BsmAI GTCTC 6 cut(s) 213, 307, 2048, 2246, 2439, 2560
BsmBI CGTCTC 2 cut(s) 213, 307
BsmFI GGGAC 2 cut(s) 521, 1955
BsmI GAATGC 1 cut(s) 2624
BsnI GGCC 3 cut(s) 439, 1499, 2206
BsoBI CYCGRG 1 cut(s) 2563
Bsp119I TTCGAA 1 cut(s) 2464
Bsp1286I GDGCHC 2 cut(s) 294, 522
Bsp68I TCGCGA 2 cut(s) 1851, 2274
BspACI CCGC 8 cut(s) 37, 140, 246, 440, 673, 758, 2005, 2422
BspANI GGCC 3 cut(s) 439, 1499, 2206
BspCNI CTCAG 2 cut(s) 556, 1063
BspFNI CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
BspHI TCATGA 1 cut(s) 1489
BspLI GGNNCC 5 cut(s) 160, 438, 1423, 2388, 2561
BspPI GGATC 7 cut(s) 39, 120, 613, 979, 1184, 1847, 2136
BspT104I TTCGAA 1 cut(s) 2464
BspT107I GGYRCC 2 cut(s) 158, 1421
BsrBI CCGCTC 2 cut(s) 246, 675
BsrDI GCAATG 2 cut(s) 260, 2650
BsrI ACTGG 9 cut(s) 187, 242, 378, 509, 729, 907, 1661, 2280, 2522
BssECI CCNNGG 2 cut(s) 143, 1999
BssNAI GTATAC 2 cut(s) 670, 2584
BssNI GRCGYC 1 cut(s) 159
Bst1107I GTATAC 2 cut(s) 670, 2584
Bst4CI ACNGT 7 cut(s) 452, 924, 932, 1000, 1206, 2438, 2606
Bst6I CTCTTC 2 cut(s) 1214, 2138
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 2015
BstBI TTCGAA 1 cut(s) 2464
BstC8I GCNNGC 4 cut(s) 959, 1928, 1980, 2391
BstDEI CTNAG 4 cut(s) 564, 588, 1071, 1407
BstDSI CCRYGG 1 cut(s) 1999
BstFNI CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
BstH2I RGCGCY 4 cut(s) 162, 1933, 1985, 2336
BstHHI GCGC 5 cut(s) 161, 957, 1932, 1984, 2335
BstMAI GTCTC 6 cut(s) 213, 307, 2048, 2246, 2439, 2560
BstMWI GCNNNNNNNGC 7 cut(s) 156, 252, 289, 315, 365, 1990, 2354
BstNSI RCATGY 1 cut(s) 1948
BstSCI CCNGG 1 cut(s) 2193
BstSFI CTRYAG 3 cut(s) 2160, 2482, 2605
BstUI CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
BstV1I GCAGC 6 cut(s) 192, 195, 277, 292, 841, 2211
BstV2I GAAGAC 2 cut(s) 328, 1941
BstX2I RGATCY 5 cut(s) 112, 1021, 1048, 2128, 2164
BstXI CCANNNNNNTGG 1 cut(s) 2359
BstYI RGATCY 5 cut(s) 112, 1021, 1048, 2128, 2164
BstZ17I GTATAC 2 cut(s) 670, 2584
Bsu36I CCTNAGG 2 cut(s) 588, 1071
BsuRI GGCC 3 cut(s) 439, 1499, 2206
BtgI CCRYGG 1 cut(s) 1999
BtgZI GCGATG 2 cut(s) 1832, 2338
BtsI GCAGTG 1 cut(s) 2025
BtsIMutI CAGTG 4 cut(s) 71, 2025, 2287, 2443
BtuMI TCGCGA 2 cut(s) 1851, 2274
Cac8I GCNNGC 4 cut(s) 959, 1928, 1980, 2391
CaiI CAGNNNCTG 2 cut(s) 998, 1667
CciI TCATGA 1 cut(s) 1489
CfoI GCGC 5 cut(s) 161, 957, 1932, 1984, 2335
Cfr13I GGNCC 3 cut(s) 137, 437, 2519
CpoI CGGWCCG 1 cut(s) 137
Csp6I GTAC 6 cut(s) 481, 514, 1356, 1395, 1422, 2425
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 8 cut(s) 698, 968, 1138, 1490, 1495, 1829, 1915, 1945
CviQI GTAC 6 cut(s) 481, 514, 1356, 1395, 1422, 2425
DdeI CTNAG 4 cut(s) 564, 588, 1071, 1407
DinI GGCGCC 1 cut(s) 160
DraI TTTAAA 1 cut(s) 1504
DriI GACNNNNNGTC 1 cut(s) 934
Eam1104I CTCTTC 2 cut(s) 1214, 2138
Eam1105I GACNNNNNGTC 1 cut(s) 934
EarI CTCTTC 2 cut(s) 1214, 2138
EciI GGCGGA 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 137, 2519
Eco47III AGCGCT 1 cut(s) 2334
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 3 cut(s) 757, 1002, 2175
Eco72I CACGTG 1 cut(s) 2015
Eco81I CCTNAGG 2 cut(s) 588, 1071
Eco88I CYCGRG 1 cut(s) 2563
EcoICRI GAGCTC 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 294
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
Esp3I CGTCTC 2 cut(s) 213, 307
FaeI CATG 8 cut(s) 701, 971, 1141, 1493, 1498, 1832, 1918, 1948
FaqI GGGAC 2 cut(s) 521, 1955
FatI CATG 8 cut(s) 697, 967, 1137, 1489, 1494, 1828, 1914, 1944
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 1 cut(s) 2086
FblI GTMKAC 5 cut(s) 538, 669, 1453, 2071, 2583
FriOI GRGCYC 1 cut(s) 294
FspBI CTAG 5 cut(s) 216, 351, 1382, 1433, 2336
GlaI GCGC 5 cut(s) 160, 956, 1931, 1983, 2334
GsuI CTGGAG 3 cut(s) 1619, 1689, 2263
HaeII RGCGCY 4 cut(s) 162, 1933, 1985, 2336
HaeIII GGCC 3 cut(s) 439, 1499, 2206
HapII CCGG 3 cut(s) 200, 660, 2195
HhaI GCGC 5 cut(s) 161, 957, 1932, 1984, 2335
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 8 cut(s) 701, 971, 1141, 1493, 1498, 1832, 1918, 1948
Hin6I GCGC 5 cut(s) 159, 955, 1930, 1982, 2333
HinP1I GCGC 5 cut(s) 159, 955, 1930, 1982, 2333
HincII GTYRAC 2 cut(s) 539, 1454
HindII GTYRAC 2 cut(s) 539, 1454
HindIII AAGCTT 3 cut(s) 1197, 1290, 1904
HpaII CCGG 3 cut(s) 200, 660, 2195
HphI GGTGA 5 cut(s) 509, 764, 1194, 1322, 2096
Hpy166II GTNNAC 9 cut(s) 539, 670, 1078, 1153, 1379, 1454, 1567, 2072, 2584
Hpy8I GTNNAC 9 cut(s) 539, 670, 1078, 1153, 1379, 1454, 1567, 2072, 2584
Hpy99I CGWCG 5 cut(s) 107, 230, 543, 1777, 2519
HpyAV CCTTC 6 cut(s) 280, 622, 1071, 1301, 1442, 2525
HpyCH4III ACNGT 7 cut(s) 452, 924, 932, 1000, 1206, 2438, 2606
HpyCH4IV ACGT 4 cut(s) 105, 228, 2014, 2098
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 252, 289, 315, 365, 1990, 2354
HpyF3I CTNAG 4 cut(s) 564, 588, 1071, 1407
HpySE526I ACGT 4 cut(s) 105, 228, 2014, 2098
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 8 cut(s) 701, 971, 1141, 1493, 1498, 1832, 1918, 1948
HspAI GCGC 5 cut(s) 159, 955, 1930, 1982, 2333
KasI GGCGCC 1 cut(s) 158
KpnI GGTACC 1 cut(s) 1425
Ksp22I TGATCA 1 cut(s) 2086
LmnI GCTCC 6 cut(s) 147, 193, 289, 1600, 2232, 2386
Lsp1109I GCAGC 6 cut(s) 192, 195, 277, 292, 841, 2211
LweI GCATC 4 cut(s) 991, 1535, 1669, 2067
MaeI CTAG 5 cut(s) 216, 351, 1382, 1433, 2336
MaeII ACGT 4 cut(s) 105, 228, 2014, 2098
MaeIII GTNAC 2 cut(s) 610, 631
MbiI CCGCTC 2 cut(s) 246, 675
MfeI CAATTG 1 cut(s) 837
MflI RGATCY 5 cut(s) 112, 1021, 1048, 2128, 2164
MhlI GDGCHC 2 cut(s) 294, 522
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 3 cut(s) 2253, 2270, 2466
MmeI TCCRAC 3 cut(s) 171, 1935, 2497
MroXI GAANNNNTTC 1 cut(s) 1992
MslI CAYNNNNRTG 1 cut(s) 1827
MspI CCGG 3 cut(s) 200, 660, 2195
MspR9I CCNGG 1 cut(s) 2195
MunI CAATTG 1 cut(s) 837
Mva1269I GAATGC 1 cut(s) 2624
MvnI CGCG 5 cut(s) 760, 957, 1118, 1851, 2274
MwoI GCNNNNNNNGC 7 cut(s) 156, 252, 289, 315, 365, 1990, 2354
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 2195
NlaIII CATG 8 cut(s) 701, 971, 1141, 1493, 1498, 1832, 1918, 1948
NlaIV GGNNCC 5 cut(s) 160, 438, 1423, 2388, 2561
NmuCI GTSAC 1 cut(s) 631
NruI TCGCGA 2 cut(s) 1851, 2274
NspI RCATGY 1 cut(s) 1948
NspV TTCGAA 1 cut(s) 2464
PagI TCATGA 1 cut(s) 1489
PciI ACATGT 1 cut(s) 1944
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 2624
PdmI GAANNNNTTC 1 cut(s) 1992
PfeI GAWTC 7 cut(s) 46, 431, 880, 886, 1510, 1516, 2535
PflMI CCANNNNNTGG 1 cut(s) 1667
PleI GAGTC 3 cut(s) 2253, 2270, 2465
PluTI GGCGCC 1 cut(s) 162
PmaCI CACGTG 1 cut(s) 2015
PmlI CACGTG 1 cut(s) 2015
PpsI GAGTC 3 cut(s) 2253, 2270, 2465
Ppu21I YACGTR 1 cut(s) 2015
PscI ACATGT 1 cut(s) 1944
PshBI ATTAAT 1 cut(s) 1793
Psp124BI GAGCTC 1 cut(s) 294
PspCI CACGTG 1 cut(s) 2015
PspN4I GGNNCC 5 cut(s) 160, 438, 1423, 2388, 2561
PspPI GGNCC 3 cut(s) 137, 437, 2519
PstNI CAGNNNCTG 2 cut(s) 998, 1667
PsuI RGATCY 5 cut(s) 112, 1021, 1048, 2128, 2164
RruI TCGCGA 2 cut(s) 1851, 2274
RsaI GTAC 6 cut(s) 482, 515, 1357, 1396, 1423, 2426
RsaNI GTAC 6 cut(s) 481, 514, 1356, 1395, 1422, 2425
RseI CAYNNNNRTG 1 cut(s) 1827
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 2 cut(s) 537, 1452
Sau96I GGNCC 3 cut(s) 137, 437, 2519
SchI GAGTC 3 cut(s) 2253, 2270, 2466
ScrFI CCNGG 1 cut(s) 2195
SduI GDGCHC 2 cut(s) 294, 522
SfaNI GCATC 4 cut(s) 991, 1535, 1669, 2067
SfcI CTRYAG 3 cut(s) 2160, 2482, 2605
SfoI GGCGCC 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 2464
SinI GGWCC 2 cut(s) 137, 2519
SmiMI CAYNNNNRTG 1 cut(s) 1827
SmlI CTYRAG 1 cut(s) 1278
SmoI CTYRAG 1 cut(s) 1278
SpeI ACTAGT 1 cut(s) 1381
SsiI CCGC 8 cut(s) 37, 140, 246, 440, 673, 758, 2005, 2422
SspDI GGCGCC 1 cut(s) 158
SspI AATATT 2 cut(s) 1732, 2110
SspMI CTAG 5 cut(s) 216, 351, 1382, 1433, 2336
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 1 cut(s) 2193
TaaI ACNGT 7 cut(s) 452, 924, 932, 1000, 1206, 2438, 2606
TaiI ACGT 4 cut(s) 108, 231, 2017, 2101
TaqII GACCGA 3 cut(s) 125, 163, 1548
TatI WGTACW 1 cut(s) 1394
TauI GCSGC 4 cut(s) 39, 442, 760, 2008
TfiI GAWTC 7 cut(s) 46, 431, 880, 886, 1510, 1516, 2535
TscAI CASTG 4 cut(s) 78, 2025, 2287, 2443
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 6 cut(s) 180, 183, 265, 280, 854, 2224
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 6 cut(s) 475, 1082, 1461, 1835, 1845, 2391
TspGWI ACGGA 4 cut(s) 353, 556, 795, 1761
TspRI CASTG 4 cut(s) 78, 2025, 2287, 2443
Van91I CCANNNNNTGG 1 cut(s) 1667
VpaK11BI GGWCC 2 cut(s) 137, 2519
VspI ATTAAT 1 cut(s) 1793
XapI RAATTY 6 cut(s) 403, 546, 1401, 1635, 1645, 2502
XceI RCATGY 1 cut(s) 1948
XmiI GTMKAC 5 cut(s) 538, 669, 1453, 2071, 2583
XmnI GAANNNNTTC 1 cut(s) 1992
XspI CTAG 5 cut(s) 216, 351, 1382, 1433, 2336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.