Rh2DG113400

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011468
Physical Location & Seq
Forward (+)
9362013 .. 9367116
5104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG113400.1

Sequence Viewer

Length: 1905 bp
ATGAAGCACCACCACCAGCACCATGCCGCCACCTGTAAACTCCTCCTACTCCTCCTCCTCCTCATCCTCGGCTCCACAGGCCCAAACGCCACCGTTTCGGCCTCCGCGCTTCTGCGATCGCAGCCGAACCACGACGCCGGCCTCTCTCGCTCTCCCAGCCGATCGCTCCTCTCCCATCCACCGCCTAAGCGTAGCACTCAATTTGTGGCGGCGCCGGAGGGGAAGTTACGGTTAGTGGACACTGATTCCGGCGCAGTAATTTATACTGTGGGTTTAGGGGCGCCGCTGTACAGTTCATACCAGGCTCCTCAGGGCAAAGATAACAATAGGTTCCACGTAGATGTTGATGAAGATGGCGAGGTCTATTTGGTCAGTAATGATATCAAACAGAACCTGCATATTCCAATCTCAGAGTTTGTCCACAAGACGCCTTATGCATTAGAGGATGGATCAGTTACAGTTGGATCGAAGAAGACCACTACCTTTGTGATTGATCCGATGACCGGAAGGACAATTCACACATACACTTCATCTCGTGGAGAGGAGCAGGGTCTTCTGTACCTTGGTTTTATTAAAGGCATTAAGCAATGGATCAAGCCTGTTCCAACCAATCCAAATGCTGCTAAGGCGCTACTATATGTCCTGGTGACAGAGTATTCCTTGAAGGCATACTTTCCACATTCAAATGAAGTCTCATGGAATTTGACATATTCTGAAATTGGAGTTATTTCATTTTGTTCGGATATTGAGAGTCCGGTTAGTGGGGCCTTTGGTTCTGAAGCAGGCAGTGATATTGTTTTACCGTTATCATGTCAATCAAAAATAAGAGTCCGCCATCAAAATCGTATATCGATTGAACCATCTAGACATGGCAGATTGTTAGAGGAACATAACGCAGATAAGATCCTCTCAGATCCCACTTCAAATGCAATGCCTGTTGTGAGAAAATCTCCTAAAGGCATGTTAGTCATGCTTTTTGATTGGTCACGAGTTTTGACTCTCAGTTTCTCCTTTATCATCCTCATGGCCTTTGTTATATACTGCTATACTTATGTAGTCAAAAGGCAACAGCTCAGTAATTCTGATTCCAATACTGCACCTTCCAAGAGAAGGAAGAGTCGGAAATCAGAAAAGAATGCTAGTGGCATTGGTGAAGAAAAGGAGAAACATGTATCATCTCAGGATGAGGAAGCTTTAGCACACTCTGAGGGTGATAACAAAACATTGTTCCTTGATAAACTATTTTATGGTGCTGCAGTCGGTCGCAGGATTGGTAAACTACTTGTATCAAATACAGAGATCGCCAAGGGAAGCAATGGCACCATTGTCCTGGAGGGAGTTTATGAAGGTCGACCTGTTGCAGTCAAACGCCTTGTTCTAGCTCATCATGACGTGGCTTTTAAAGAAATTCAGAATCTTATTGCATCTGACCAACATCAGAACATAGTCCGATGGTACGGTGTGGAGTACGATCGAGATTTTGTCTATCTCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTACTCAAATTCCGGTGAAAATCCTGTAGCTGGTGAGGAATATGCTATGATTCAAAATAAAGTTCGCTTGTCAACAGTAAAGAATCTTATGCCGAACGTTAACTTGTGGAAAGAAAATGGTCTATTGTCCCCCCTATTGTTGAAATTGATGAGGGATGTCGTCTGTGGGCTTGTGCATTTACATGAATTGGGGATAATTCATAGGGACTTAAAGCCTCAAAATGTTTTGTTAATTAAGGAAAGTTCATTTTGTGCAAAGCTTTCTGACATGGGCATCAGCAAGCGACTTATTGGGGACATGTCTTCTTTGGGTCATCATTCTACTGGAAAGTACTCTTTACTTGTCTCACTTGCGAACTTTAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

634

Amino Acids

70.31

Weight (kDa)

8.82

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 431 - 609 5.7e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 432 - 611 1.9e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 402
AccB1I GGYRCC 3 cut(s) 211, 280, 1319
AccI GTMKAC 1 cut(s) 1351
AccII CGCG 1 cut(s) 107
AciI CCGC 6 cut(s) 27, 105, 182, 209, 284, 832
AclI AACGTT 1 cut(s) 1632
AclWI GGATC 6 cut(s) 457, 472, 488, 599, 898, 908
AcsI RAATTY 4 cut(s) 700, 1407, 1533, 1543
AcuI CTGAAG 1 cut(s) 798
AcyI GRCGYC 4 cut(s) 135, 212, 281, 428
AfaI GTAC 5 cut(s) 290, 560, 1457, 1469, 1868
AfiI CCNNNNNNNGG 4 cut(s) 503, 761, 1110, 1565
AflIII ACRYGT 2 cut(s) 1168, 1833
AgsI TTSAA 6 cut(s) 664, 684, 857, 924, 1589, 1678
AjiI CACGTC 1 cut(s) 1393
AjnI CCWGG 3 cut(s) 300, 642, 1329
AluBI AGCT 5 cut(s) 1072, 1193, 1382, 1565, 1795
AluI AGCT 5 cut(s) 1072, 1193, 1382, 1565, 1795
Alw26I GTCTC 2 cut(s) 697, 1885
AlwI GGATC 6 cut(s) 457, 472, 488, 599, 898, 908
AlwNI CAGNNNCTG 1 cut(s) 394
AoxI GGCC 5 cut(s) 79, 99, 139, 765, 1026
ApeKI GCWGC 3 cut(s) 121, 620, 1253
ApoI RAATTY 4 cut(s) 700, 1407, 1533, 1543
AsiSI GCGATCGC 1 cut(s) 119
AspLEI GCGC 5 cut(s) 109, 214, 254, 283, 631
AspS9I GGNCC 2 cut(s) 80, 765
AsuHPI GGTGA 5 cut(s) 658, 1163, 1223, 1562, 1580
AxyI CCTNAGG 1 cut(s) 309
BanI GGYRCC 3 cut(s) 211, 280, 1319
BauI CACGAG 2 cut(s) 534, 987
BbsI GAAGAC 3 cut(s) 479, 545, 1830
BbvI GCAGC 3 cut(s) 133, 607, 1240
BccI CCATC 6 cut(s) 183, 347, 440, 843, 868, 1446
BciT130I CCWGG 3 cut(s) 302, 644, 1331
BcoDI GTCTC 2 cut(s) 697, 1885
BfaI CTAG 3 cut(s) 864, 1140, 1379
BfmI CTRYAG 2 cut(s) 1254, 1560
BfoI RGCGCY 3 cut(s) 215, 284, 632
BfuAI ACCTGC 1 cut(s) 402
BisI GCNGC 6 cut(s) 27, 122, 210, 284, 621, 1254
BlsI GCNGC 6 cut(s) 28, 123, 211, 285, 622, 1255
BmcAI AGTACT 1 cut(s) 1868
Bme1390I CCNGG 3 cut(s) 302, 644, 1331
BmgBI CACGTC 1 cut(s) 1393
BmgT120I GGNCC 2 cut(s) 80, 765
BmiI GGNNCC 7 cut(s) 73, 213, 282, 306, 332, 766, 1321
BmrFI CCNGG 3 cut(s) 302, 644, 1331
BmsI GCATC 2 cut(s) 1433, 1818
BpiI GAAGAC 3 cut(s) 479, 545, 1830
BplI GAGNNNNNCTC 2 cut(s) 934, 966
BpmI CTGGAG 2 cut(s) 1352, 1517
Bpu10I CCTNAGC 2 cut(s) 186, 624
Bsa29I ATCGAT 1 cut(s) 851
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 4 cut(s) 135, 212, 281, 428
BsaJI CCNNGG 3 cut(s) 67, 562, 1305
BsaWI WCCGGW 3 cut(s) 503, 754, 1547
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 209, 239
Bsc4I CCNNNNNNNGG 4 cut(s) 503, 761, 1110, 1565
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 1864
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 3 cut(s) 593, 936, 1321
BseBI CCWGG 3 cut(s) 302, 644, 1331
BseCI ATCGAT 1 cut(s) 851
BseDI CCNNGG 3 cut(s) 67, 562, 1305
BseGI GGATG 7 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696
BseLI CCNNNNNNNGG 4 cut(s) 503, 761, 1110, 1565
BseMI GCAATG 3 cut(s) 593, 936, 1321
BseMII CTCAG 7 cut(s) 323, 423, 924, 1015, 1087, 1193, 1197
BseNI ACTGG 1 cut(s) 1864
BseRI GAGGAG 8 cut(s) 32, 41, 44, 47, 50, 158, 297, 557
BseXI GCAGC 3 cut(s) 133, 607, 1240
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 4 cut(s) 119, 164, 1264, 1474
BshFI GGCC 5 cut(s) 81, 101, 141, 767, 1028
BshNI GGYRCC 3 cut(s) 211, 280, 1319
BshVI ATCGAT 1 cut(s) 851
BsiEI CGRYCG 4 cut(s) 119, 164, 1264, 1474
BsiSI CCGG 6 cut(s) 138, 215, 249, 504, 755, 1548
BslFI GGGAC 3 cut(s) 1648, 1754, 1844
BslI CCNNNNNNNGG 4 cut(s) 503, 761, 1110, 1565
BsmAI GTCTC 2 cut(s) 697, 1885
BsmFI GGGAC 3 cut(s) 1648, 1754, 1844
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 5 cut(s) 81, 101, 141, 767, 1028
Bsp1407I TGTACA 1 cut(s) 288
BspACI CCGC 6 cut(s) 27, 105, 182, 209, 284, 832
BspANI GGCC 5 cut(s) 81, 101, 141, 767, 1028
BspCNI CTCAG 7 cut(s) 322, 422, 923, 1014, 1086, 1192, 1198
BspDI ATCGAT 1 cut(s) 851
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 1387
BspLI GGNNCC 7 cut(s) 73, 213, 282, 306, 332, 766, 1321
BspMAI CTGCAG 1 cut(s) 1258
BspMI ACCTGC 1 cut(s) 402
BspPI GGATC 6 cut(s) 457, 472, 488, 599, 898, 908
BspT107I GGYRCC 3 cut(s) 211, 280, 1319
BsrDI GCAATG 3 cut(s) 593, 936, 1321
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 1864
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 3 cut(s) 67, 562, 1305
BssNI GRCGYC 4 cut(s) 135, 212, 281, 428
BssSI CACGAG 2 cut(s) 534, 987
BssT1I CCWWGG 2 cut(s) 562, 1305
Bst2BI CACGAG 2 cut(s) 534, 987
Bst2UI CCWGG 3 cut(s) 302, 644, 1331
Bst4CI ACNGT 8 cut(s) 94, 231, 268, 293, 460, 804, 1460, 1612
Bst6I CTCTTC 1 cut(s) 1109
BstACI GRCGYC 4 cut(s) 135, 212, 281, 428
BstAUI TGTACA 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 3 cut(s) 139, 784, 1817
BstDEI CTNAG 9 cut(s) 186, 309, 409, 624, 910, 1001, 1073, 1179, 1206
BstF5I GGATG 7 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 3 cut(s) 215, 284, 632
BstHHI GCGC 5 cut(s) 109, 214, 254, 283, 631
BstMAI GTCTC 2 cut(s) 697, 1885
BstMCI CGRYCG 4 cut(s) 119, 164, 1264, 1474
BstMWI GCNNNNNNNGC 5 cut(s) 78, 121, 147, 156, 626
BstNI CCWGG 3 cut(s) 302, 644, 1331
BstNSI RCATGY 3 cut(s) 964, 1172, 1837
BstSCI CCNGG 3 cut(s) 300, 642, 1329
BstSFI CTRYAG 2 cut(s) 1254, 1560
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 133, 607, 1240
BstV2I GAAGAC 3 cut(s) 479, 545, 1830
BstX2I RGATCY 2 cut(s) 903, 913
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 2 cut(s) 903, 913
Bsu15I ATCGAT 1 cut(s) 851
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 5 cut(s) 81, 101, 141, 767, 1028
BsuTUI ATCGAT 1 cut(s) 851
BtrI CACGTC 1 cut(s) 1393
BtsCI GGATG 7 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 2 cut(s) 240, 793
BveI ACCTGC 1 cut(s) 402
Cac8I GCNNGC 3 cut(s) 139, 784, 1817
CaiI CAGNNNCTG 1 cut(s) 394
CciI TCATGA 1 cut(s) 1387
CfoI GCGC 5 cut(s) 109, 214, 254, 283, 631
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 80, 765
ClaI ATCGAT 1 cut(s) 851
CseI GACGC 2 cut(s) 143, 436
Csp6I GTAC 5 cut(s) 289, 559, 1456, 1468, 1867
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviQI GTAC 5 cut(s) 289, 559, 1456, 1468, 1867
DdeI CTNAG 9 cut(s) 186, 309, 409, 624, 910, 1001, 1073, 1179, 1206
DinI GGCGCC 2 cut(s) 213, 282
DraI TTTAAA 2 cut(s) 1402, 1897
Eam1104I CTCTTC 1 cut(s) 1109
EarI CTCTTC 1 cut(s) 1109
EciI GGCGGA 1 cut(s) 821
Eco130I CCWWGG 2 cut(s) 562, 1305
Eco32I GATATC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 798
Eco81I CCTNAGG 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 765
EcoRII CCWGG 3 cut(s) 300, 642, 1329
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 562, 1305
EcoT22I ATGCAT 1 cut(s) 439
EgeI GGCGCC 2 cut(s) 213, 282
EheI GGCGCC 2 cut(s) 213, 282
ErhI CCWWGG 2 cut(s) 562, 1305
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 3 cut(s) 1648, 1754, 1844
FblI GTMKAC 1 cut(s) 1351
Fnu4HI GCNGC 6 cut(s) 27, 122, 210, 284, 621, 1254
FokI GGATG 7 cut(s) 50, 162, 458, 1004, 1196, 1532, 1703
Fsp4HI GCNGC 6 cut(s) 27, 122, 210, 284, 621, 1254
FspBI CTAG 3 cut(s) 864, 1140, 1379
GlaI GCGC 5 cut(s) 108, 213, 253, 282, 630
GluI GCNGC 6 cut(s) 27, 122, 210, 284, 621, 1254
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 2 cut(s) 1352, 1517
HaeII RGCGCY 3 cut(s) 215, 284, 632
HaeIII GGCC 5 cut(s) 81, 101, 141, 767, 1028
HapII CCGG 6 cut(s) 138, 215, 249, 504, 755, 1548
HgaI GACGC 2 cut(s) 143, 436
HhaI GCGC 5 cut(s) 109, 214, 254, 283, 631
Hin1I GRCGYC 4 cut(s) 135, 212, 281, 428
Hin6I GCGC 5 cut(s) 107, 212, 252, 281, 629
HinP1I GCGC 5 cut(s) 107, 212, 252, 281, 629
HincII GTYRAC 3 cut(s) 1352, 1608, 1636
HindII GTYRAC 3 cut(s) 1352, 1608, 1636
HindIII AAGCTT 2 cut(s) 1191, 1793
HinfI GANTC 9 cut(s) 245, 751, 828, 997, 1085, 1117, 1414, 1585, 1618
HpaI GTTAAC 1 cut(s) 1636
HpaII CCGG 6 cut(s) 138, 215, 249, 504, 755, 1548
HphI GGTGA 5 cut(s) 658, 1163, 1223, 1562, 1580
Hpy166II GTNNAC 7 cut(s) 38, 238, 421, 1277, 1352, 1608, 1636
Hpy188III TCNNGA 6 cut(s) 864, 987, 1181, 1388, 1475, 1496
Hpy8I GTNNAC 7 cut(s) 38, 238, 421, 1277, 1352, 1608, 1636
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 5 cut(s) 501, 658, 1104, 1110, 1340
HpyCH4III ACNGT 8 cut(s) 94, 231, 268, 293, 460, 804, 1460, 1612
HpyCH4IV ACGT 3 cut(s) 336, 1392, 1632
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 121, 147, 156, 626
HpyF3I CTNAG 9 cut(s) 186, 309, 409, 624, 910, 1001, 1073, 1179, 1206
HpySE526I ACGT 3 cut(s) 336, 1392, 1632
Hsp92I GRCGYC 4 cut(s) 135, 212, 281, 428
HspAI GCGC 5 cut(s) 107, 212, 252, 281, 629
KasI GGCGCC 2 cut(s) 211, 280
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
KspAI GTTAAC 1 cut(s) 1636
LmnI GCTCC 5 cut(s) 77, 171, 310, 544, 1498
Lsp1109I GCAGC 3 cut(s) 133, 607, 1240
LweI GCATC 2 cut(s) 1433, 1818
MaeI CTAG 3 cut(s) 864, 1140, 1379
MaeII ACGT 3 cut(s) 336, 1392, 1632
MaeIII GTNAC 4 cut(s) 225, 454, 646, 984
MboII GAAGA 7 cut(s) 362, 481, 484, 545, 1126, 1166, 1830
MflI RGATCY 2 cut(s) 903, 913
Mly113I GGCGCC 2 cut(s) 212, 281
MlyI GAGTC 4 cut(s) 760, 837, 991, 1126
MmeI TCCRAC 3 cut(s) 442, 629, 1100
Mph1103I ATGCAT 1 cut(s) 439
MroNI GCCGGC 1 cut(s) 137
MseI TTAA 9 cut(s) 573, 582, 1401, 1635, 1745, 1766, 1770, 1896, 1903
MslI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 6 cut(s) 138, 215, 249, 504, 755, 1548
MspR9I CCNGG 3 cut(s) 302, 644, 1331
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 3 cut(s) 302, 644, 1331
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 5 cut(s) 78, 121, 147, 156, 626
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 2 cut(s) 212, 281
NgoMIV GCCGGC 1 cut(s) 137
NlaIV GGNNCC 7 cut(s) 73, 213, 282, 306, 332, 766, 1321
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 646, 984
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 3 cut(s) 964, 1172, 1837
PacI TTAATTAA 1 cut(s) 1770
PagI TCATGA 1 cut(s) 1387
PciI ACATGT 2 cut(s) 1168, 1833
PctI GAATGC 1 cut(s) 1141
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 5 cut(s) 245, 1085, 1414, 1585, 1618
PfoI TCCNGGA 1 cut(s) 1329
PkrI GCNGC 6 cut(s) 28, 123, 211, 285, 622, 1255
Ple19I CGATCG 3 cut(s) 119, 164, 1474
PleI GAGTC 4 cut(s) 759, 836, 991, 1125
PluTI GGCGCC 2 cut(s) 215, 284
PpsI GAGTC 4 cut(s) 759, 836, 991, 1125
Ppu21I YACGTR 1 cut(s) 337
PscI ACATGT 2 cut(s) 1168, 1833
Psp1406I AACGTT 1 cut(s) 1632
Psp6I CCWGG 3 cut(s) 300, 642, 1329
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 3 cut(s) 300, 642, 1329
PspN4I GGNNCC 7 cut(s) 73, 213, 282, 306, 332, 766, 1321
PspPI GGNCC 2 cut(s) 80, 765
PstI CTGCAG 1 cut(s) 1258
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 903, 913
PvuI CGATCG 3 cut(s) 119, 164, 1474
RgaI GCGATCGC 1 cut(s) 119
RsaI GTAC 5 cut(s) 290, 560, 1457, 1469, 1868
RsaNI GTAC 5 cut(s) 289, 559, 1456, 1468, 1867
RseI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
SalI GTCGAC 1 cut(s) 1350
SaqAI TTAA 9 cut(s) 573, 582, 1401, 1635, 1745, 1766, 1770, 1896, 1903
SatI GCNGC 6 cut(s) 27, 122, 210, 284, 621, 1254
Sau96I GGNCC 2 cut(s) 80, 765
ScaI AGTACT 1 cut(s) 1868
SchI GAGTC 4 cut(s) 760, 837, 991, 1126
ScrFI CCNGG 3 cut(s) 302, 644, 1331
SfaAI GCGATCGC 1 cut(s) 119
SfaNI GCATC 2 cut(s) 1433, 1818
SfcI CTRYAG 2 cut(s) 1254, 1560
SfoI GGCGCC 2 cut(s) 213, 282
SgfI GCGATCGC 1 cut(s) 119
SmiMI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
SsiI CCGC 6 cut(s) 27, 105, 182, 209, 284, 832
SspDI GGCGCC 2 cut(s) 211, 280
SspMI CTAG 3 cut(s) 864, 1140, 1379
StyD4I CCNGG 3 cut(s) 300, 642, 1329
StyI CCWWGG 2 cut(s) 562, 1305
TaaI ACNGT 8 cut(s) 94, 231, 268, 293, 460, 804, 1460, 1612
TaiI ACGT 3 cut(s) 339, 1395, 1635
TaqI TCGA 4 cut(s) 467, 851, 1351, 1474
TaqII GACCGA 1 cut(s) 1250
TatI WGTACW 2 cut(s) 288, 1866
TauI GCSGC 3 cut(s) 29, 212, 286
TfiI GAWTC 5 cut(s) 245, 1085, 1414, 1585, 1618
Tru1I TTAA 9 cut(s) 573, 582, 1401, 1635, 1745, 1766, 1770, 1896, 1903
Tru9I TTAA 9 cut(s) 573, 582, 1401, 1635, 1745, 1766, 1770, 1896, 1903
TscAI CASTG 2 cut(s) 247, 793
TseFI GTSAC 2 cut(s) 646, 984
TseI GCWGC 3 cut(s) 121, 620, 1253
Tsp45I GTSAC 2 cut(s) 646, 984
TspRI CASTG 2 cut(s) 247, 793
XapI RAATTY 4 cut(s) 700, 1407, 1533, 1543
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 3 cut(s) 964, 1172, 1837
XmiI GTMKAC 1 cut(s) 1351
XspI CTAG 3 cut(s) 864, 1140, 1379
ZrmI AGTACT 1 cut(s) 1868
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.