Rw2G008540

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011468
Physical Location & Seq
Forward (+)
8499460 .. 8503388
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008540.1

Sequence Viewer

Length: 2556 bp
ATGAAGCACCACCACCAGCACCATGCCGCCACCTGGAAACTCCTCCTCCTCCTCCTCCTCATCCTCGGCTCCACAGGCCCAAACGCCGCCGTTTCGGCCTCCGCGCTTCTGCGATCGCAGCCGAACCACAACGTCGGCCTCTCTCGCTCTCCCAGCCGATCGCTCCTCTCCCATCCACCGCCAAAGCGTAGCACTCAATTTGTGGCGGTGCCGGAGGGGAAGTTACGGTTAGTGGACACTGATTCCGGCGCAGTAATTTATACTGTGGATTTAGGGGCGCTGCTGTACAGTTCATACCAGGCTCCTCAAGGCAAAGATAACAAAAGGTTCCACGTAGATGTTGATGAAGATGGCGAGGTCTATTTGGTCAGTAATGATATCAAACAGAACCTGCATATTCCAATCTCAGAGTTTGTCCACAAGACGCCTTATACATTAGAGGATGGATCAGTTACTGTTGGATCGAAGAAGACCACTACCTTTGTGATTGATCCGATGACCGGAAGGATAATTCACACATACACTTCATCTCGCGGAGAGGAGCAGGGTCTTCTGTACCCTGGTTTTATTAAAGGCATTAAGCAATGGATCAAGCCTGTTCCAACCAATCCAAATGCTGCTAAGGCGCTACTATATGTCCTGATGACAGAGTATTTCTTGAAGGCATACTTTCCACATTCAAATGAAGTCTCATGGAATTTGACGTATTCTGAAATTGGAGTTATTTCATTTTGTTCAGATATTGAGAGTCCGGTTAGTGGGGCCTTTGGTTCTGAAGCAGGCAGTGATATTGTTTTACCGTTATCATGTCAATCAAAAATAAGAGTCCGCCATCAAAATCGTATATCGATTGAACCATCCAGACATGGCAGATTGTTAGAGGAACATAACACAGATAAGATCCTCTCAGATCCCACTTCAAATGCAATGCCTGTTGTGAGAAAATCTCCTAAAGGCATGTTAGTCATGCTTTTTGATTGGTCACGAGTTTTGACTCTCAGTTTCTCCTTTATCATCCTCATGGGCTTTGTTATATACTGCTATACTTATGTAGTTAAAAGGCAACAGCTCAGTAATTCTGATTCAAATACTGCACCTTCCAAGAGAAGGAAGAGTCGGAAATCAGAAAAGAATGCTAGTGGCATTGGTGAAGAAAAGGAGAAACATGTATCATCTCAGGATGAGGAAGCTTTAGCACACTCTGAGGGTGATAACAAAACATTGTTCCTTGATAAACTATTTTATGGTGCTGCCGTCGGTCGCAGGATTGGTAAACTACTTGTATCAAATACAGAGATCGCCAAGGGAAGCAATGGCACCATTGTCCTGGAGGGAGTTTATGAAGGTCGACCTGTTGCAGTCAAACGCCTTGTTCTAGCTCATCATGACGTGGCTTTTAAAGAAATTCAGAATCTTATTGCATCTGACCAACATCAGAACATAGTCCGATGGTACGGTGTGGAGTACGATCGAGATTTTGTCTATCTCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTACTCAAATTCCGGTGAAAATCCTGTAGCTGGTGAGGAATATGCTATCATTCAAAATAAAGTTCGCTTGTCAACAGTAAAGAATCTTATGCCGAACGTTAATTTGTGGAAAGAAAATGGTCTATTGTCCCCCCTATTGTTGAAATTGATGAGGGATGTCGTCTGTGGGCTTGTGCATTTACATGAATTGGGGATAATTCATAGGGACTTAAAGCCTCAAAATGTTTTGTTAATTAAGGAAAGTTCATTTTGTGCAAAGCTTTCTGACATGGGCATCAGCAAGCGACTTATTGGGGACATGTCTTCTTTGGGTTCTGGTAGTTCGGGTTGGCAAGCACCTGAACAGCTTCTTCACGGGCGGCAAACACGTGCAGTGGATTTGTTTAGTTTAGGTTGTGTCCTCTTCTTTTGCATCACTGGTGGTAGACATCCATTTGGTGATCGCCTCGAACGTGATATAAATATTGTGAAGAACCAAATGGATCTCTTCTTGGTGGAGTATATTCCGGAAGCTTTGGATCTTATATCTCGATTGTTAAATCGTGACCCTGAATTGAGGCCAAAGGCTTTGGAGGTGTTGCACCATCCTCTTTTCTGGAGCTCTGAGTTGAGACTCTCATTCCTGCGAGACTATAGTGACAGGGTAGAACTGGAAGATAGACAGGCTAACTCTGATCTTCTGAAAGCCCTAGAAAGTACCGCGCCCGTGGCTTTGGGTGGGAAATGGAATGAAAAGATGGATCCTGCATTCCTCACTAACATTGGCCATTACAGGCGTTATAAGTTTGACAGTGTCCGGGACTTACTGCGAGTCATGCGGAACAAATATAATCACTATAGAGAGCTCCCAAAGGAAATCCAGGAACTCCTAGGTCCAGTGCCCGAAGGATATGATCACTACTTTGCAAGTCGGTTCCCAAAACTTCTGATTGAAGTATACAAAGTTGCGTGCAAACATTGTAGAGAAGAGGAATGGTTTCAGAAGTATTTCAAAAGCACTGCCAATGAGTCCTCGGGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

851

Amino Acids

96.11

Weight (kDa)

8.49

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 430 - 703 9.1e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 431 - 709 7.6e-34 Protein kinase domain
Ribonuc_2-5A PF06479 715 - 841 8.6e-47 Ribonuclease 2-5A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2313
Acc36I ACCTGC 1 cut(s) 399
AccB1I GGYRCC 2 cut(s) 208, 1316
AccI GTMKAC 3 cut(s) 1348, 1957, 2469
AccII CGCG 3 cut(s) 104, 534, 2234
AccIII TCCGGA 1 cut(s) 2038
AclI AACGTT 1 cut(s) 1629
AcoI YGGCCR 1 cut(s) 2296
AcsI RAATTY 4 cut(s) 697, 1404, 1530, 1540
AcuI CTGAAG 1 cut(s) 795
AcvI CACGTG 1 cut(s) 1901
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 5 cut(s) 287, 557, 1454, 1466, 2230
AfiI CCNNNNNNNGG 5 cut(s) 33, 500, 758, 1107, 1562
AflIII ACRYGT 3 cut(s) 1165, 1830, 1898
AgsI TTSAA 9 cut(s) 661, 681, 854, 921, 1086, 1586, 1675, 2465, 2524
AjiI CACGTC 1 cut(s) 1390
AjnI CCWGG 5 cut(s) 32, 297, 559, 1326, 2391
AluBI AGCT 9 cut(s) 1069, 1190, 1379, 1562, 1792, 1879, 2045, 2133, 2377
AluI AGCT 9 cut(s) 1069, 1190, 1379, 1562, 1792, 1879, 2045, 2133, 2377
Alw21I GWGCWC 2 cut(s) 2135, 2379
Alw26I GTCTC 3 cut(s) 694, 2137, 2154
AlwNI CAGNNNCTG 2 cut(s) 391, 455
Ama87I CYCGRG 1 cut(s) 2545
Aor13HI TCCGGA 1 cut(s) 2038
AoxI GGCC 6 cut(s) 76, 96, 136, 762, 2090, 2296
ApeKI GCWGC 4 cut(s) 118, 280, 617, 1250
ApoI RAATTY 4 cut(s) 697, 1404, 1530, 1540
AsiSI GCGATCGC 1 cut(s) 116
Asp700I GAANNNNTTC 3 cut(s) 1878, 2508, 2519
AspA2I CCTAGG 1 cut(s) 2401
AspLEI GCGC 5 cut(s) 106, 251, 280, 628, 2236
AspS9I GGNCC 3 cut(s) 77, 762, 2405
AsuC2I CCSGG 1 cut(s) 2330
AsuHPI GGTGA 5 cut(s) 1160, 1220, 1559, 1577, 1982
AvaI CYCGRG 1 cut(s) 2545
AvaII GGWCC 1 cut(s) 2405
AvrII CCTAGG 1 cut(s) 2401
BaeGI GKGCMC 1 cut(s) 2415
BalI TGGCCA 1 cut(s) 2298
BamHI GGATCC 1 cut(s) 2272
BanI GGYRCC 2 cut(s) 208, 1316
BanII GRGCYC 2 cut(s) 2135, 2379
BauI CACGAG 1 cut(s) 984
BbrPI CACGTG 1 cut(s) 1901
BbsI GAAGAC 3 cut(s) 476, 542, 1827
Bbv12I GWGCWC 2 cut(s) 2135, 2379
BbvI GCAGC 4 cut(s) 130, 267, 604, 1237
BccI CCATC 8 cut(s) 180, 344, 437, 840, 865, 1443, 2124, 2263
BceAI ACGGC 2 cut(s) 74, 1238
BciT130I CCWGG 5 cut(s) 34, 299, 561, 1328, 2393
BclI TGATCA 1 cut(s) 2425
BcnI CCSGG 1 cut(s) 2330
BcoDI GTCTC 3 cut(s) 694, 2137, 2154
BfaI CTAG 5 cut(s) 1137, 1376, 2222, 2402, 2554
BfmI CTRYAG 3 cut(s) 1557, 2164, 2368
BfoI RGCGCY 2 cut(s) 281, 629
BfuAI ACCTGC 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 7 cut(s) 27, 87, 119, 281, 618, 1251, 1892
BlnI CCTAGG 1 cut(s) 2401
BlsI GCNGC 7 cut(s) 28, 88, 120, 282, 619, 1252, 1893
Bme1390I CCNGG 6 cut(s) 34, 299, 561, 1328, 2330, 2393
Bme18I GGWCC 1 cut(s) 2405
BmeT110I CYCGRG 1 cut(s) 2545
BmgBI CACGTC 1 cut(s) 1390
BmgT120I GGNCC 3 cut(s) 77, 762, 2405
BmiI GGNNCC 8 cut(s) 70, 210, 303, 329, 763, 1318, 2274, 2447
BmrFI CCNGG 6 cut(s) 34, 299, 561, 1328, 2330, 2393
BmsI GCATC 3 cut(s) 1430, 1815, 1953
BpiI GAAGAC 3 cut(s) 476, 542, 1827
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 3 cut(s) 1349, 1514, 2149
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 2330
Bsa29I ATCGAT 1 cut(s) 848
BsaAI YACGTR 2 cut(s) 334, 1901
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 6 cut(s) 64, 559, 1302, 2238, 2401, 2544
BsaWI WCCGGW 4 cut(s) 500, 751, 1544, 2038
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 206, 236
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 500, 758, 1107, 1562
Bse1I ACTGG 3 cut(s) 1954, 2187, 2408
Bse3DI GCAATG 3 cut(s) 590, 933, 1318
BseAI TCCGGA 1 cut(s) 2038
BseBI CCWGG 5 cut(s) 34, 299, 561, 1328, 2393
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 6 cut(s) 64, 559, 1302, 2238, 2401, 2544
BseLI CCNNNNNNNGG 5 cut(s) 33, 500, 758, 1107, 1562
BseMI GCAATG 3 cut(s) 590, 933, 1318
BseMII CTCAG 7 cut(s) 420, 921, 1012, 1084, 1190, 1194, 2127
BseNI ACTGG 3 cut(s) 1954, 2187, 2408
BseRI GAGGAG 9 cut(s) 32, 35, 38, 41, 44, 47, 155, 294, 554
BseSI GKGCMC 1 cut(s) 2415
BseXI GCAGC 4 cut(s) 130, 267, 604, 1237
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 2 cut(s) 1077, 1923
Bsh1236I CGCG 3 cut(s) 104, 534, 2234
Bsh1285I CGRYCG 4 cut(s) 116, 161, 1261, 1471
BshFI GGCC 6 cut(s) 78, 98, 138, 764, 2092, 2298
BshNI GGYRCC 2 cut(s) 208, 1316
BshVI ATCGAT 1 cut(s) 848
BsiEI CGRYCG 4 cut(s) 116, 161, 1261, 1471
BsiHKAI GWGCWC 2 cut(s) 2135, 2379
BsiHKCI CYCGRG 1 cut(s) 2545
BsiSI CCGG 7 cut(s) 212, 246, 501, 752, 1545, 2039, 2329
BslFI GGGAC 4 cut(s) 1645, 1751, 1841, 2345
BslI CCNNNNNNNGG 5 cut(s) 33, 500, 758, 1107, 1562
BsmAI GTCTC 3 cut(s) 694, 2137, 2154
BsmFI GGGAC 4 cut(s) 1645, 1751, 1841, 2345
BsmI GAATGC 2 cut(s) 1138, 2279
BsnI GGCC 6 cut(s) 78, 98, 138, 764, 2092, 2298
BsoBI CYCGRG 1 cut(s) 2545
Bsp1286I GDGCHC 3 cut(s) 2135, 2379, 2415
Bsp13I TCCGGA 1 cut(s) 2038
Bsp1407I TGTACA 1 cut(s) 285
BspANI GGCC 6 cut(s) 78, 98, 138, 764, 2092, 2298
BspCNI CTCAG 7 cut(s) 419, 920, 1011, 1083, 1189, 1195, 2128
BspDI ATCGAT 1 cut(s) 848
BspEI TCCGGA 1 cut(s) 2038
BspFNI CGCG 3 cut(s) 104, 534, 2234
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 8 cut(s) 70, 210, 303, 329, 763, 1318, 2274, 2447
BspMI ACCTGC 1 cut(s) 399
BspT107I GGYRCC 2 cut(s) 208, 1316
BsrDI GCAATG 3 cut(s) 590, 933, 1318
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 3 cut(s) 1954, 2187, 2408
BssECI CCNNGG 6 cut(s) 64, 559, 1302, 2238, 2401, 2544
BssNAI GTATAC 1 cut(s) 2470
BssNI GRCGYC 1 cut(s) 425
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 2 cut(s) 1302, 2401
Bst1107I GTATAC 1 cut(s) 2470
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 5 cut(s) 34, 299, 561, 1328, 2393
Bst4CI ACNGT 8 cut(s) 228, 265, 290, 457, 801, 1457, 1609, 2324
Bst6I CTCTTC 4 cut(s) 1106, 1940, 2024, 2493
BstACI GRCGYC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 2 cut(s) 334, 1901
BstC8I GCNNGC 4 cut(s) 781, 1814, 1866, 2482
BstDEI CTNAG 8 cut(s) 406, 621, 907, 998, 1070, 1176, 1203, 2136
BstDSI CCRYGG 1 cut(s) 2238
BstFNI CGCG 3 cut(s) 104, 534, 2234
BstH2I RGCGCY 2 cut(s) 281, 629
BstHHI GCGC 5 cut(s) 106, 251, 280, 628, 2236
BstMAI GTCTC 3 cut(s) 694, 2137, 2154
BstMCI CGRYCG 4 cut(s) 116, 161, 1261, 1471
BstMWI GCNNNNNNNGC 8 cut(s) 75, 95, 118, 144, 153, 623, 2240, 2347
BstNI CCWGG 5 cut(s) 34, 299, 561, 1328, 2393
BstNSI RCATGY 3 cut(s) 961, 1169, 1834
BstSCI CCNGG 6 cut(s) 32, 297, 559, 1326, 2328, 2391
BstSFI CTRYAG 3 cut(s) 1557, 2164, 2368
BstSLI GKGCMC 1 cut(s) 2415
BstUI CGCG 3 cut(s) 104, 534, 2234
BstV1I GCAGC 4 cut(s) 130, 267, 604, 1237
BstV2I GAAGAC 3 cut(s) 476, 542, 1827
BstX2I RGATCY 5 cut(s) 900, 910, 2014, 2050, 2272
BstXI CCANNNNNNTGG 1 cut(s) 1327
BstYI RGATCY 5 cut(s) 900, 910, 2014, 2050, 2272
BstZ17I GTATAC 1 cut(s) 2470
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 6 cut(s) 78, 98, 138, 764, 2092, 2298
BsuTUI ATCGAT 1 cut(s) 848
BtgI CCRYGG 1 cut(s) 2238
BtrI CACGTC 1 cut(s) 1390
BtsI GCAGTG 3 cut(s) 790, 1911, 2529
BtsIMutI CAGTG 7 cut(s) 237, 790, 1911, 1947, 2329, 2415, 2529
BveI ACCTGC 1 cut(s) 399
Cac8I GCNNGC 4 cut(s) 781, 1814, 1866, 2482
CaiI CAGNNNCTG 2 cut(s) 391, 455
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 5 cut(s) 106, 251, 280, 628, 2236
Cfr13I GGNCC 3 cut(s) 77, 762, 2405
ClaI ATCGAT 1 cut(s) 848
CseI GACGC 1 cut(s) 433
Csp6I GTAC 5 cut(s) 286, 556, 1453, 1465, 2229
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviQI GTAC 5 cut(s) 286, 556, 1453, 1465, 2229
DdeI CTNAG 8 cut(s) 406, 621, 907, 998, 1070, 1176, 1203, 2136
DraI TTTAAA 1 cut(s) 1399
EaeI YGGCCR 1 cut(s) 2296
Eam1104I CTCTTC 4 cut(s) 1106, 1940, 2024, 2493
EarI CTCTTC 4 cut(s) 1106, 1940, 2024, 2493
EciI GGCGGA 1 cut(s) 818
Ecl136II GAGCTC 2 cut(s) 2133, 2377
Eco130I CCWWGG 2 cut(s) 1302, 2401
Eco24I GRGCYC 2 cut(s) 2135, 2379
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 2405
Eco53kI GAGCTC 2 cut(s) 2133, 2377
Eco57I CTGAAG 1 cut(s) 795
Eco72I CACGTG 1 cut(s) 1901
Eco88I CYCGRG 1 cut(s) 2545
EcoICRI GAGCTC 2 cut(s) 2133, 2377
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 5 cut(s) 32, 297, 559, 1326, 2391
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 1302, 2401
EcoT38I GRGCYC 2 cut(s) 2135, 2379
ErhI CCWWGG 2 cut(s) 1302, 2401
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 4 cut(s) 1645, 1751, 1841, 2345
FbaI TGATCA 1 cut(s) 2425
FblI GTMKAC 3 cut(s) 1348, 1957, 2469
Fnu4HI GCNGC 7 cut(s) 27, 87, 119, 281, 618, 1251, 1892
FriOI GRGCYC 2 cut(s) 2135, 2379
Fsp4HI GCNGC 7 cut(s) 27, 87, 119, 281, 618, 1251, 1892
FspBI CTAG 5 cut(s) 1137, 1376, 2222, 2402, 2554
GlaI GCGC 5 cut(s) 105, 250, 279, 627, 2235
GluI GCNGC 7 cut(s) 27, 87, 119, 281, 618, 1251, 1892
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 3 cut(s) 1349, 1514, 2149
HaeII RGCGCY 2 cut(s) 281, 629
HaeIII GGCC 6 cut(s) 78, 98, 138, 764, 2092, 2298
HapII CCGG 7 cut(s) 212, 246, 501, 752, 1545, 2039, 2329
HgaI GACGC 1 cut(s) 433
HhaI GCGC 5 cut(s) 106, 251, 280, 628, 2236
Hin1I GRCGYC 1 cut(s) 425
Hin6I GCGC 5 cut(s) 104, 249, 278, 626, 2234
HinP1I GCGC 5 cut(s) 104, 249, 278, 626, 2234
HincII GTYRAC 2 cut(s) 1349, 1605
HindII GTYRAC 2 cut(s) 1349, 1605
HindIII AAGCTT 3 cut(s) 1188, 1790, 2043
HpaII CCGG 7 cut(s) 212, 246, 501, 752, 1545, 2039, 2329
HphI GGTGA 5 cut(s) 1160, 1220, 1559, 1577, 1982
Hpy166II GTNNAC 7 cut(s) 235, 418, 1274, 1349, 1605, 1958, 2470
Hpy8I GTNNAC 7 cut(s) 235, 418, 1274, 1349, 1605, 1958, 2470
Hpy99I CGWCG 2 cut(s) 137, 1259
HpyAV CCTTC 6 cut(s) 498, 655, 1101, 1107, 1337, 2411
HpyCH4III ACNGT 8 cut(s) 228, 265, 290, 457, 801, 1457, 1609, 2324
HpyCH4IV ACGT 7 cut(s) 132, 333, 704, 1389, 1629, 1900, 1984
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 95, 118, 144, 153, 623, 2240, 2347
HpyF3I CTNAG 8 cut(s) 406, 621, 907, 998, 1070, 1176, 1203, 2136
HpySE526I ACGT 7 cut(s) 132, 333, 704, 1389, 1629, 1900, 1984
Hsp92I GRCGYC 1 cut(s) 425
HspAI GCGC 5 cut(s) 104, 249, 278, 626, 2234
Kpn2I TCCGGA 1 cut(s) 2038
Ksp22I TGATCA 1 cut(s) 2425
LmnI GCTCC 7 cut(s) 74, 168, 307, 541, 1495, 2130, 2382
Lsp1109I GCAGC 4 cut(s) 130, 267, 604, 1237
LweI GCATC 3 cut(s) 1430, 1815, 1953
MaeI CTAG 5 cut(s) 1137, 1376, 2222, 2402, 2554
MaeII ACGT 7 cut(s) 132, 333, 704, 1389, 1629, 1900, 1984
MaeIII GTNAC 5 cut(s) 222, 451, 981, 2075, 2168
MflI RGATCY 5 cut(s) 900, 910, 2014, 2050, 2272
MhlI GDGCHC 3 cut(s) 2135, 2379, 2415
MlsI TGGCCA 1 cut(s) 2298
MluNI TGGCCA 1 cut(s) 2298
MlyI GAGTC 8 cut(s) 757, 834, 988, 1123, 2139, 2352, 2544, 2549
MmeI TCCRAC 3 cut(s) 439, 626, 1097
Mox20I TGGCCA 1 cut(s) 2298
MroI TCCGGA 1 cut(s) 2038
MroXI GAANNNNTTC 3 cut(s) 1878, 2508, 2519
MscI TGGCCA 1 cut(s) 2298
MseI TTAA 9 cut(s) 570, 579, 1056, 1398, 1632, 1742, 1763, 1767, 2069
MslI CAYNNNNRTG 4 cut(s) 336, 681, 1019, 1713
Msp20I TGGCCA 1 cut(s) 2298
MspI CCGG 7 cut(s) 212, 246, 501, 752, 1545, 2039, 2329
MspR9I CCNGG 6 cut(s) 34, 299, 561, 1328, 2330, 2393
Mva1269I GAATGC 2 cut(s) 1138, 2279
MvaI CCWGG 5 cut(s) 34, 299, 561, 1328, 2393
MvnI CGCG 3 cut(s) 104, 534, 2234
MwoI GCNNNNNNNGC 8 cut(s) 75, 95, 118, 144, 153, 623, 2240, 2347
NciI CCSGG 1 cut(s) 2330
NlaIV GGNNCC 8 cut(s) 70, 210, 303, 329, 763, 1318, 2274, 2447
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 3 cut(s) 981, 2075, 2168
NspI RCATGY 3 cut(s) 961, 1169, 1834
PacI TTAATTAA 1 cut(s) 1767
PagI TCATGA 1 cut(s) 1384
PciI ACATGT 2 cut(s) 1165, 1830
PcsI WCGNNNNNNNCGW 1 cut(s) 1981
PctI GAATGC 2 cut(s) 1138, 2279
PdmI GAANNNNTTC 3 cut(s) 1878, 2508, 2519
PfeI GAWTC 4 cut(s) 242, 1082, 1411, 1615
PflFI GACNNNGTC 1 cut(s) 2324
PfoI TCCNGGA 3 cut(s) 1326, 2328, 2391
PkrI GCNGC 7 cut(s) 28, 88, 120, 282, 619, 1252, 1893
Ple19I CGATCG 3 cut(s) 116, 161, 1471
PleI GAGTC 8 cut(s) 756, 833, 988, 1122, 2139, 2351, 2544, 2548
PmaCI CACGTG 1 cut(s) 1901
PmlI CACGTG 1 cut(s) 1901
PpsI GAGTC 8 cut(s) 756, 833, 988, 1122, 2139, 2351, 2544, 2548
Ppu21I YACGTR 2 cut(s) 334, 1901
PscI ACATGT 2 cut(s) 1165, 1830
PsiI TTATAA 1 cut(s) 2313
Psp124BI GAGCTC 2 cut(s) 2135, 2379
Psp1406I AACGTT 1 cut(s) 1629
Psp6I CCWGG 5 cut(s) 32, 297, 559, 1326, 2391
PspCI CACGTG 1 cut(s) 1901
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 5 cut(s) 32, 297, 559, 1326, 2391
PspN4I GGNNCC 8 cut(s) 70, 210, 303, 329, 763, 1318, 2274, 2447
PspPI GGNCC 3 cut(s) 77, 762, 2405
PstNI CAGNNNCTG 2 cut(s) 391, 455
PsuI RGATCY 5 cut(s) 900, 910, 2014, 2050, 2272
PsyI GACNNNGTC 1 cut(s) 2324
PvuI CGATCG 3 cut(s) 116, 161, 1471
RgaI GCGATCGC 1 cut(s) 116
RsaI GTAC 5 cut(s) 287, 557, 1454, 1466, 2230
RsaNI GTAC 5 cut(s) 286, 556, 1453, 1465, 2229
RseI CAYNNNNRTG 4 cut(s) 336, 681, 1019, 1713
SacI GAGCTC 2 cut(s) 2135, 2379
SalI GTCGAC 1 cut(s) 1347
SaqAI TTAA 9 cut(s) 570, 579, 1056, 1398, 1632, 1742, 1763, 1767, 2069
SatI GCNGC 7 cut(s) 27, 87, 119, 281, 618, 1251, 1892
Sau96I GGNCC 3 cut(s) 77, 762, 2405
SchI GAGTC 8 cut(s) 757, 834, 988, 1123, 2139, 2352, 2544, 2549
ScrFI CCNGG 6 cut(s) 34, 299, 561, 1328, 2330, 2393
SduI GDGCHC 3 cut(s) 2135, 2379, 2415
SfaAI GCGATCGC 1 cut(s) 116
SfaNI GCATC 3 cut(s) 1430, 1815, 1953
SfcI CTRYAG 3 cut(s) 1557, 2164, 2368
SgfI GCGATCGC 1 cut(s) 116
SinI GGWCC 1 cut(s) 2405
SmiMI CAYNNNNRTG 4 cut(s) 336, 681, 1019, 1713
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
SspI AATATT 1 cut(s) 1996
SspMI CTAG 5 cut(s) 1137, 1376, 2222, 2402, 2554
SstI GAGCTC 2 cut(s) 2135, 2379
StyD4I CCNGG 6 cut(s) 32, 297, 559, 1326, 2328, 2391
StyI CCWWGG 2 cut(s) 1302, 2401
TaaI ACNGT 8 cut(s) 228, 265, 290, 457, 801, 1457, 1609, 2324
TaiI ACGT 7 cut(s) 135, 336, 707, 1392, 1632, 1903, 1987
TaqI TCGA 6 cut(s) 464, 848, 1348, 1471, 1980, 2062
TaqII GACCGA 1 cut(s) 1247
TatI WGTACW 1 cut(s) 285
TauI GCSGC 3 cut(s) 29, 89, 1894
TfiI GAWTC 4 cut(s) 242, 1082, 1411, 1615
Tru1I TTAA 9 cut(s) 570, 579, 1056, 1398, 1632, 1742, 1763, 1767, 2069
Tru9I TTAA 9 cut(s) 570, 579, 1056, 1398, 1632, 1742, 1763, 1767, 2069
TscAI CASTG 7 cut(s) 244, 790, 1911, 1954, 2329, 2415, 2536
TseFI GTSAC 3 cut(s) 981, 2075, 2168
TseI GCWGC 4 cut(s) 118, 280, 617, 1250
Tsp45I GTSAC 3 cut(s) 981, 2075, 2168
TspRI CASTG 7 cut(s) 244, 790, 1911, 1954, 2329, 2415, 2536
Tth111I GACNNNGTC 1 cut(s) 2324
VpaK11BI GGWCC 1 cut(s) 2405
XapI RAATTY 4 cut(s) 697, 1404, 1530, 1540
XceI RCATGY 3 cut(s) 961, 1169, 1834
XmaJI CCTAGG 1 cut(s) 2401
XmiI GTMKAC 3 cut(s) 1348, 1957, 2469
XmnI GAANNNNTTC 3 cut(s) 1878, 2508, 2519
XspI CTAG 5 cut(s) 1137, 1376, 2222, 2402, 2554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.