pycom02g08880

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011468
Physical Location & Seq
Forward (+)
6141865 .. 6145406
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08880.2

Sequence Viewer

Length: 2154 bp
ATGAGAATACGAAAATGCGGAGAAAATCATTCACGAGACATACATCCCGACAGGCAACGCAGCCGTACGGAGTCGGAGATCCTAAGAGTGGATGGTCCGCCGACGTTTCGAATCTCCGACGCTGTCTTCTCCCCAAAATCCAAAAACCCCTCAGCTAAATCATCGATACGCGTCATGTTCTTCTCCATCCTATCTCCGCCGATGGATCGCCGCAATTCAATTCCGATATTTATCCTACTCGTAGCACTGATCGCCGGTTTTCTCACCCGAAACGCCGACGTTTCGGATCTTACCGACTTCCAGATTATCGGTCCGCCTCGGAGCTATGACGGCGCCGTTTTGAACTCGGTCAGCAGCAGCCAGTTGGAGCTTCCGGTCTTTTACCAGACTAGAGACGGCGACGTTTTGAACTGGGAGAACGGGGAGAGCAGGGCATTGCAGCCGTTGGGGTCGCAGCAGAAGGAGCTCGAAGTCGTCTCTCGCTCTCCAAGCCGAAGACTTCACTCCCATACGGAGGATGAACCTAGAACTGCATTAGTTGCTCTACTGGATGGAACAATACAATTAGTGGATAGAAATTCGATGGGAGTTAGATGGGCGTTTGATTCGGGGCCGCCAATTTACAGTTCCTATCAGGCTCAAAGCGGCAGTGGGTACTTCATATATTGTGGTGATGACTGGGACTTGTACGAGCAAAGCGATCATTTTGGTCGACGGAAAATTCCATCGACTATTGCTGAGTATGTGGATAAAAAAACGCCTAAAGTGGAGGAGGATGGATCAGTTACACTTGGACATAAGAAGGTCACCTTCTTTGAGGTTGGTCTTTATACCGGAAAGGTATACCGCTCATACACTCTATCCGAAGATAATGTTAGAGATCGAAAGAAGTTTGTCAAGCCCAGTTTGATAAACGGAAATGCTGGTGAGCTGCGAATAGTATTCTTCAGAATAGATTATTACTTGGTGTCTTACCATCCGTATTCAGAGGAAGTATCTTGGAATATGACAATTGCTGAAATCAGGGCTGCTATCCCTTGCAAAGATATTGAGAATCTGATTCGTGGTTCTAAAACTGGCAGTCAGATTGCATTACCATTTGACTGTCAGTCAAAAGCACTTGTTAAGCGCGAGCGAGATCATGGATCATTAGAGCATCAACAGGCAGACACTGTGGTCAAAAAGTCTGCTTCAGATCTGGTGGTTCCTTCAGAGCCTGAGGTTCACAATATGTTTCTTCATTGGTCAAAAGCATTTGCTCTTGCGTTTTTGTTTGTAATCCTCATGGGCTTTGTTGTTTACCACTTTGTTTTTCTGGTTAATGGTCAAAAGCCTGATCCGAAAGCTTCACCTTCCAAGAGGAAGAAGAATCGGAAATTGGGAAAAAATGGAATTGTTGAAAGAAAAGAGGAAAGTGTATTATCTCAAGATGAGGAAGCTTTAACAAACACCGAAGGTGATAACAAGATATTGCCATTTCTTAACAAACTCTTTGATGGTGGTACTAATGGTCGCAGGATTGGTAAACTAGTGATTTCAAGTACAGAAATTTCCAAGGGAAGTAATGGTACCATTGTCCTAGAGGGAGTTTATGAAGGTCGACCTGTTGCTGTAAAACGCCTTGTATTAGCCCATCATGACATGGCTTTTAAAGAGATTCAGAATCTTATTGCATCTGACCGACACCCAAACATAGTAAGATGGTATGGAGTGGACTATGATAAAGATTTTGTCTATATCTCTCTGGAGCGATGTATATGCAACTTGGATGATTTGATACAAATTTGCCCAAATTCGTCTCAAAATCCAGTAGCTGGAGAAGAAGATGCAAAACGAGTTCTGATTGAAAACGAAGTTCGCTTGAAGTCATTGAAGAATATTATGTCAGATGTTAACTTGTGGAAAGTAGATGGCTTTCCGTCACTTCTACTATTGAAATTAATGAGGGATGTGGTTTCTGGTCTTGTGCATTTGCATGAAATGGGGATCATTCATCGTGACTTAAAGCCTCAAAATGTTTTGTTGATTAAGGAAAGAACCTTATGTGCAAAGCTTTCCGACATGGGTATTAGCAAGCGCCTCATCGGGGACATGTCTTCTTTGGGTCATCTTGCCACTGGAAAGTTGTGGCAGTTCGGGTTGGCAAGCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

718

Amino Acids

80.75

Weight (kDa)

8.62

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1175
Acc65I GGTACC 1 cut(s) 1568
AccB1I GGYRCC 2 cut(s) 332, 1568
AccB7I CCANNNNNTGG 1 cut(s) 1814
AccBSI CCGCTC 1 cut(s) 849
AccI GTMKAC 3 cut(s) 712, 843, 1600
AccII CGCG 2 cut(s) 171, 1131
AciI CCGC 8 cut(s) 18, 98, 197, 211, 314, 614, 645, 847
AclWI GGATC 7 cut(s) 73, 213, 294, 787, 1153, 1331, 1994
AcsI RAATTY 5 cut(s) 577, 720, 1548, 1782, 1792
AcuI CTGAAG 3 cut(s) 931, 1176, 1194
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 6 cut(s) 67, 656, 689, 1504, 1543, 1570
AfiI CCNNNNNNNGG 4 cut(s) 88, 514, 1814, 2086
AflIII ACRYGT 2 cut(s) 169, 2091
AgsI TTSAA 9 cut(s) 219, 343, 409, 1400, 1539, 1847, 1864, 1873, 1936
AhdI GACNNNNNGTC 1 cut(s) 1108
AhlI ACTAGT 1 cut(s) 1528
AjuI GAANNNNNNNTTGG 4 cut(s) 610, 642, 1361, 1393
AluBI AGCT 9 cut(s) 155, 324, 370, 466, 931, 1346, 1439, 1814, 2053
AluI AGCT 9 cut(s) 155, 324, 370, 466, 931, 1346, 1439, 1814, 2053
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 4 cut(s) 30, 387, 481, 1803
AlwI GGATC 7 cut(s) 73, 213, 294, 787, 1153, 1331, 1994
AlwNI CAGNNNCTG 3 cut(s) 1172, 1217, 1814
AoxI GGCC 1 cut(s) 611
ApeKI GCWGC 7 cut(s) 60, 354, 357, 439, 454, 931, 1028
ApoI RAATTY 5 cut(s) 577, 720, 1548, 1782, 1792
AseI ATTAAT 1 cut(s) 1940
Asp718I GGTACC 1 cut(s) 1568
AspLEI GCGC 4 cut(s) 335, 1131, 2079, 2150
AspS9I GGNCC 3 cut(s) 95, 311, 611
AsuHPI GGTGA 6 cut(s) 256, 683, 799, 938, 1341, 1469
AsuII TTCGAA 1 cut(s) 109
AvaII GGWCC 2 cut(s) 95, 311
AxyI CCTNAGG 1 cut(s) 1218
BaeI ACNNNNGTAYC 2 cut(s) 1560, 1593
BanI GGYRCC 2 cut(s) 332, 1568
BanII GRGCYC 1 cut(s) 468
BarI GAAGNNNNNNTAC 2 cut(s) 1552, 1584
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 3 cut(s) 118, 502, 2088
Bbv12I GWGCWC 1 cut(s) 468
BbvCI CCTCAGC 1 cut(s) 151
BbvI GCAGC 7 cut(s) 72, 366, 369, 451, 466, 918, 1015
BceAI ACGGC 5 cut(s) 48, 320, 346, 412, 427
BcgI CGANNNNNNTGC 2 cut(s) 1740, 1774
BcoDI GTCTC 4 cut(s) 30, 387, 481, 1803
BcuI ACTAGT 1 cut(s) 1528
BfaI CTAG 4 cut(s) 390, 525, 1529, 1580
BfoI RGCGCY 3 cut(s) 336, 2080, 2151
BglII AGATCT 1 cut(s) 1195
Bme18I GGWCC 2 cut(s) 95, 311
BmeRI GACNNNNNGTC 1 cut(s) 1108
BmgT120I GGNCC 3 cut(s) 95, 311, 611
BmiI GGNNCC 4 cut(s) 334, 612, 1206, 1570
BmrI ACTGGG 3 cut(s) 421, 688, 897
BmsI GCATC 3 cut(s) 1165, 1682, 1816
BmuI ACTGGG 3 cut(s) 421, 688, 897
BpiI GAAGAC 3 cut(s) 118, 502, 2088
BpmI CTGGAG 2 cut(s) 1766, 1836
Bpu10I CCTNAGC 1 cut(s) 151
Bpu14I TTCGAA 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 1410
Bsa29I ATCGAT 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 317, 1554
BsaWI WCCGGW 2 cut(s) 373, 833
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 514, 1814, 2086
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 8 cut(s) 361, 416, 552, 683, 903, 1081, 1808, 2122
Bse21I CCTNAGG 1 cut(s) 1218
Bse3DI GCAATG 1 cut(s) 434
Bse8I GATNNNNATC 1 cut(s) 230
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 317, 1554
BseGI GGATG 9 cut(s) 43, 97, 186, 523, 556, 781, 976, 1774, 1954
BseJI GATNNNNATC 1 cut(s) 230
BseLI CCNNNNNNNGG 4 cut(s) 88, 514, 1814, 2086
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 3 cut(s) 165, 729, 1209
BseNI ACTGG 8 cut(s) 361, 416, 552, 683, 903, 1081, 1808, 2122
BseRI GAGGAG 1 cut(s) 785
BseXI GCAGC 7 cut(s) 72, 366, 369, 451, 466, 918, 1015
Bsh1236I CGCG 2 cut(s) 171, 1131
BshFI GGCC 1 cut(s) 613
BshNI GGYRCC 2 cut(s) 332, 1568
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 3 cut(s) 255, 374, 834
BsiWI CGTACG 1 cut(s) 65
BslFI GGGAC 2 cut(s) 695, 2102
BslI CCNNNNNNNGG 4 cut(s) 88, 514, 1814, 2086
BsmAI GTCTC 4 cut(s) 30, 387, 481, 1803
BsmBI CGTCTC 3 cut(s) 387, 481, 1803
BsmFI GGGAC 2 cut(s) 695, 2102
BsnI GGCC 1 cut(s) 613
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 468
BspACI CCGC 8 cut(s) 18, 98, 197, 211, 314, 614, 645, 847
BspANI GGCC 1 cut(s) 613
BspCNI CTCAG 3 cut(s) 164, 730, 1210
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 2 cut(s) 171, 1131
BspHI TCATGA 1 cut(s) 1636
BspLI GGNNCC 4 cut(s) 334, 612, 1206, 1570
BspPI GGATC 7 cut(s) 73, 213, 294, 787, 1153, 1331, 1994
BspT104I TTCGAA 1 cut(s) 109
BspT107I GGYRCC 2 cut(s) 332, 1568
BsrBI CCGCTC 1 cut(s) 849
BsrDI GCAATG 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 8 cut(s) 361, 416, 552, 683, 903, 1081, 1808, 2122
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 317, 1554
BssNAI GTATAC 1 cut(s) 844
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 1554
Bst1107I GTATAC 1 cut(s) 844
Bst2BI CACGAG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 626, 1106, 1174
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 539
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 1133, 2075, 2146
BstDEI CTNAG 4 cut(s) 83, 151, 738, 1218
BstEII GGTNACC 1 cut(s) 805
BstF5I GGATG 9 cut(s) 43, 97, 186, 523, 556, 781, 976, 1774, 1954
BstFNI CGCG 2 cut(s) 171, 1131
BstH2I RGCGCY 3 cut(s) 336, 2080, 2151
BstHHI GCGC 4 cut(s) 335, 1131, 2079, 2150
BstMAI GTCTC 4 cut(s) 30, 387, 481, 1803
BstMWI GCNNNNNNNGC 5 cut(s) 251, 330, 463, 489, 539
BstNSI RCATGY 1 cut(s) 2095
BstPI GGTNACC 1 cut(s) 805
BstUI CGCG 2 cut(s) 171, 1131
BstV1I GCAGC 7 cut(s) 72, 366, 369, 451, 466, 918, 1015
BstV2I GAAGAC 3 cut(s) 118, 502, 2088
BstX2I RGATCY 3 cut(s) 78, 286, 1195
BstYI RGATCY 3 cut(s) 78, 286, 1195
BstZ17I GTATAC 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 1218
BsuRI GGCC 1 cut(s) 613
BsuTUI ATCGAT 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 1765
BtsCI GGATG 9 cut(s) 43, 97, 186, 523, 556, 781, 976, 1774, 1954
BtsI GCAGTG 1 cut(s) 655
BtsIMutI CAGTG 4 cut(s) 245, 655, 1170, 2115
Cac8I GCNNGC 3 cut(s) 1133, 2075, 2146
CaiI CAGNNNCTG 3 cut(s) 1172, 1217, 1814
CciI TCATGA 1 cut(s) 1636
CfoI GCGC 4 cut(s) 335, 1131, 2079, 2150
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 3 cut(s) 95, 311, 611
ClaI ATCGAT 1 cut(s) 164
CpoI CGGWCCG 1 cut(s) 311
CseI GACGC 2 cut(s) 128, 160
Csp6I GTAC 6 cut(s) 66, 655, 688, 1503, 1542, 1569
CspI CGGWCCG 1 cut(s) 311
CviAII CATG 8 cut(s) 175, 1142, 1285, 1637, 1642, 1976, 2062, 2092
CviQI GTAC 6 cut(s) 66, 655, 688, 1503, 1542, 1569
DdeI CTNAG 4 cut(s) 83, 151, 738, 1218
DinI GGCGCC 1 cut(s) 334
DraI TTTAAA 1 cut(s) 1651
DrdI GACNNNNNNGTC 1 cut(s) 1175
DriI GACNNNNNGTC 1 cut(s) 1108
DseDI GACNNNNNNGTC 1 cut(s) 1175
Eam1105I GACNNNNNGTC 1 cut(s) 1108
EciI GGCGGA 3 cut(s) 87, 186, 303
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 1554
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 95, 311
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 3 cut(s) 931, 1176, 1194
Eco81I CCTNAGG 1 cut(s) 1218
Eco91I GGTNACC 1 cut(s) 805
EcoICRI GAGCTC 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 1554
EcoT38I GRGCYC 1 cut(s) 468
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 1554
Esp3I CGTCTC 3 cut(s) 387, 481, 1803
FaeI CATG 8 cut(s) 178, 1145, 1288, 1640, 1645, 1979, 2065, 2095
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 2 cut(s) 695, 2102
FatI CATG 8 cut(s) 174, 1141, 1284, 1636, 1641, 1975, 2061, 2091
FblI GTMKAC 3 cut(s) 712, 843, 1600
FokI GGATG 9 cut(s) 30, 104, 173, 530, 563, 788, 963, 1781, 1961
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 4 cut(s) 390, 525, 1529, 1580
GlaI GCGC 4 cut(s) 334, 1130, 2078, 2149
GsuI CTGGAG 2 cut(s) 1766, 1836
HaeII RGCGCY 3 cut(s) 336, 2080, 2151
HaeIII GGCC 1 cut(s) 613
HapII CCGG 3 cut(s) 255, 374, 834
HgaI GACGC 2 cut(s) 128, 160
HhaI GCGC 4 cut(s) 335, 1131, 2079, 2150
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 8 cut(s) 178, 1145, 1288, 1640, 1645, 1979, 2065, 2095
Hin6I GCGC 4 cut(s) 333, 1129, 2077, 2148
HinP1I GCGC 4 cut(s) 333, 1129, 2077, 2148
HincII GTYRAC 3 cut(s) 713, 1601, 1894
HindII GTYRAC 3 cut(s) 713, 1601, 1894
HindIII AAGCTT 3 cut(s) 1344, 1437, 2051
HinfI GANTC 8 cut(s) 71, 111, 605, 1054, 1060, 1369, 1657, 1663
HpaI GTTAAC 1 cut(s) 1894
HpaII CCGG 3 cut(s) 255, 374, 834
HphI GGTGA 6 cut(s) 256, 683, 799, 938, 1341, 1469
Hpy166II GTNNAC 8 cut(s) 713, 844, 1225, 1300, 1526, 1601, 1714, 1894
Hpy188III TCNNGA 7 cut(s) 33, 47, 301, 1427, 1637, 1745, 1997
Hpy8I GTNNAC 8 cut(s) 713, 844, 1225, 1300, 1526, 1601, 1714, 1894
Hpy99I CGWCG 5 cut(s) 106, 122, 281, 404, 717
HpyAV CCTTC 7 cut(s) 454, 796, 820, 1218, 1362, 1448, 1589
HpyCH4III ACNGT 3 cut(s) 626, 1106, 1174
HpyCH4IV ACGT 3 cut(s) 104, 279, 402
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 330, 463, 489, 539
HpyF3I CTNAG 4 cut(s) 83, 151, 738, 1218
HpySE526I ACGT 3 cut(s) 104, 279, 402
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 8 cut(s) 178, 1145, 1288, 1640, 1645, 1979, 2065, 2095
HspAI GCGC 4 cut(s) 333, 1129, 2077, 2148
KasI GGCGCC 1 cut(s) 332
KpnI GGTACC 1 cut(s) 1572
KspAI GTTAAC 1 cut(s) 1894
LmnI GCTCC 4 cut(s) 321, 367, 463, 1747
Lsp1109I GCAGC 7 cut(s) 72, 366, 369, 451, 466, 918, 1015
LweI GCATC 3 cut(s) 1165, 1682, 1816
MaeI CTAG 4 cut(s) 390, 525, 1529, 1580
MaeII ACGT 3 cut(s) 104, 279, 402
MaeIII GTNAC 4 cut(s) 784, 805, 1920, 1997
MbiI CCGCTC 1 cut(s) 849
MfeI CAATTG 1 cut(s) 1011
MflI RGATCY 3 cut(s) 78, 286, 1195
MhlI GDGCHC 1 cut(s) 468
MluI ACGCGT 1 cut(s) 169
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 4 cut(s) 54, 141, 345, 2082
MseI TTAA 9 cut(s) 1125, 1320, 1442, 1482, 1650, 1893, 1940, 2003, 2028
MslI CAYNNNNRTG 1 cut(s) 1974
MspI CCGG 3 cut(s) 255, 374, 834
MunI CAATTG 1 cut(s) 1011
MvnI CGCG 2 cut(s) 171, 1131
MwoI GCNNNNNNNGC 5 cut(s) 251, 330, 463, 489, 539
NarI GGCGCC 1 cut(s) 333
NlaIII CATG 8 cut(s) 178, 1145, 1288, 1640, 1645, 1979, 2065, 2095
NlaIV GGNNCC 4 cut(s) 334, 612, 1206, 1570
NmuCI GTSAC 3 cut(s) 805, 1920, 1997
NspI RCATGY 1 cut(s) 2095
NspV TTCGAA 1 cut(s) 109
PagI TCATGA 1 cut(s) 1636
PciI ACATGT 1 cut(s) 2091
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PfeI GAWTC 7 cut(s) 111, 605, 1054, 1060, 1369, 1657, 1663
Pfl23II CGTACG 1 cut(s) 65
PflFI GACNNNGTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 1814
PleI GAGTC 1 cut(s) 79
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 79
PscI ACATGT 1 cut(s) 2091
PshBI ATTAAT 1 cut(s) 1940
Psp124BI GAGCTC 1 cut(s) 468
PspEI GGTNACC 1 cut(s) 805
PspLI CGTACG 1 cut(s) 65
PspN4I GGNNCC 4 cut(s) 334, 612, 1206, 1570
PspPI GGNCC 3 cut(s) 95, 311, 611
PstNI CAGNNNCTG 3 cut(s) 1172, 1217, 1814
PsuI RGATCY 3 cut(s) 78, 286, 1195
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 6 cut(s) 67, 656, 689, 1504, 1543, 1570
RsaNI GTAC 6 cut(s) 66, 655, 688, 1503, 1542, 1569
RseI CAYNNNNRTG 1 cut(s) 1974
Rsr2I CGGWCCG 1 cut(s) 311
RsrII CGGWCCG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 2 cut(s) 711, 1599
SaqAI TTAA 9 cut(s) 1125, 1320, 1442, 1482, 1650, 1893, 1940, 2003, 2028
Sau96I GGNCC 3 cut(s) 95, 311, 611
SchI GAGTC 1 cut(s) 80
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 3 cut(s) 1165, 1682, 1816
SfoI GGCGCC 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 2 cut(s) 95, 311
SmiMI CAYNNNNRTG 1 cut(s) 1974
SmlI CTYRAG 1 cut(s) 1425
SmoI CTYRAG 1 cut(s) 1425
SpeI ACTAGT 1 cut(s) 1528
SsiI CCGC 8 cut(s) 18, 98, 197, 211, 314, 614, 645, 847
SspDI GGCGCC 1 cut(s) 332
SspI AATATT 1 cut(s) 1879
SspMI CTAG 4 cut(s) 390, 525, 1529, 1580
SstI GAGCTC 1 cut(s) 468
StyI CCWWGG 1 cut(s) 1554
TaaI ACNGT 3 cut(s) 626, 1106, 1174
TaiI ACGT 3 cut(s) 107, 282, 405
TaqI TCGA 8 cut(s) 109, 164, 468, 581, 712, 728, 883, 1600
TaqII GACCGA 3 cut(s) 299, 337, 1695
TatI WGTACW 1 cut(s) 1541
TauI GCSGC 3 cut(s) 213, 616, 648
TfiI GAWTC 7 cut(s) 111, 605, 1054, 1060, 1369, 1657, 1663
Tru1I TTAA 9 cut(s) 1125, 1320, 1442, 1482, 1650, 1893, 1940, 2003, 2028
Tru9I TTAA 9 cut(s) 1125, 1320, 1442, 1482, 1650, 1893, 1940, 2003, 2028
TscAI CASTG 4 cut(s) 252, 655, 1177, 2122
TseFI GTSAC 3 cut(s) 805, 1920, 1997
TseI GCWGC 7 cut(s) 60, 354, 357, 439, 454, 931, 1028
Tsp45I GTSAC 3 cut(s) 805, 1920, 1997
TspDTI ATGAA 6 cut(s) 534, 649, 1229, 1608, 1982, 1992
TspGWI ACGGA 6 cut(s) 83, 527, 730, 930, 969, 1908
TspRI CASTG 4 cut(s) 252, 655, 1177, 2122
Tth111I GACNNNGTC 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 1814
VpaK11BI GGWCC 2 cut(s) 95, 311
VspI ATTAAT 1 cut(s) 1940
XapI RAATTY 5 cut(s) 577, 720, 1548, 1782, 1792
XceI RCATGY 1 cut(s) 2095
XmiI GTMKAC 3 cut(s) 712, 843, 1600
XspI CTAG 4 cut(s) 390, 525, 1529, 1580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.