RLG00000016688

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011468
Physical Location & Seq
Forward (+)
8998154 .. 9001998
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016688

Sequence Viewer

Length: 2559 bp
ATGAAGCACCACCACCAGCACCATGCCGCCACCTGGAAACTCCTTCTACTCCTCCTCCTCCTCCTCATCCTCGGCTCCACAGCCCCAAACGCCGCCGTTTCGCCCTCCGCGCTTCAGCGATCGCAGCCGAACCACAACGCTGGCCTCTCTCGCTCTCCCAGCCGATCTCTCCTCTCCCATCCACCGCCTAAGCGTACCACTCAATTTGTGGCGGTGCCGGAGGGGAATTTACGGTTAGTGGACACTGATTCCGGCGCAGTAATTTATACTCTGGATTTAGGGGCGCCGCTGTACAGTTCATACCAGGCTCCTCAAGGCAAAGATAACAATAGGTTCCACGTAGATGTTGATGAAGATGGCGAGGTCTGTTTGGTCAGTAATGATATCAAACAGAACCTGCATATTCCAATGGCAGAGTTTGTCCACAAGACGCCTTATACATTAGATGATGGATCAGTTACACTTGGATCGAAGAAGACCACTACCTTTGTGATTGATCCGATGACCGGAAGGAGAATTCACACATACACTTCATCTCGTGGAGAGGAGCAGAATGTTCTGTACCCTGGTTTTATTAAAAGCATTAAGCAATGGATCAAGCCTGTTCCAACCAATCCAAATGCTGCTAAGTCGCTACTATATGTCATGGTGACAGAGTATTCCTTGAAGGCATACTTTCCACATTCAAATGAAGTCTCATGGAATTTGACATATTCTGAAATTGGAGTTATTTCATTTTGTTCAGATATTGAGAGTCCGGTTAGTGGGGCCTTTGGTTCTGAAGCAGGCAGTGACATTGTTTTACCGTTATCATGTCAATCAAAAATAAGAGTCCGCCATCAAAATCGTATATCGATTGAACCATCTAGACATGGCAGATTGTTAGAGGAACATAACACAGATAAGATCCTCTCAGATCCCACTTCAAATGCAATGCATGTTGTGAGAAAATCTCCTAAAGGCATGTTAGTCGTGCTTTTTGATTGGTCACGAGTTTTGACTCTCACTTTCTCCTTTGTCATCCTCACGGGCTTTGTTATATACTGCTATACTTATTTAGTTAAAAGGCAACAGCTCAGTAATTCTGATTCAAATACTGCACCTTCCAAGAGAAGGAAGAGTCGGAAATCAGAAAAGAATGCTAGTGGCATTGGTGAAGAAAAGGAGAAACATGTATCATCTCAGGATGAGGAAGCTTTAGCACACTCTGAGGGTGATAACAAAACATTGTTCCTTGATAAACTATTTTATGGTGCTGCAGTCGGTCGCAGGATTGGTAAACTACTTGTATCAAATACAGAGATCGCCAAGGGAAGCAATGGCACCATTGTCCTGGAGGGAGTTTATGAAGGTCGACCTGTTGCAGTCAAACGCCTTGTTCTAGCTCATCATGACGTGGCTTTTAAAGAAATTCAGAATCTTATTGCATCTGACCAACATCAGAACATAGTACGATGGTATGGTGTGGAGTACGATCAAGATTTTGTCTATCTCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTACTCAAGTTCCGGTCAAAATCCTGTAGCTGGTGAGGAATATGCTATGATTCAAAATAAAGTTCGCTTGTCAACAGTAAAGAATCTTATGCCGAACGTTAATTTGTGGAAAGAAAATGGTCTATTGTCCCCCCTATTGTTGAAATTGATGAGGGATGTCGTCTGTGGGCTTGTGCATTTACATGAATTGGGGATAATTCATAGGGACTTAAAGCCTCAAAATGTTTTATTAATTAAGGAAAGTTCATTTTGTGCAAAGCTTTCTGACATGGGCATCAGCAAGCGACTTATCGGGGACATGTCTTCTTTGGGTTCTGGTAGTTCGGGTTGGCAAGCACCTGAACAGCTTCTTCATGGGCGGCAAACACGTGCAGTGGATTTGTTTAGTTTAGGTTGTGTCCTCTTCTTTTGCATCACTGGTGGTAGACATCCATTTGGTGATCACCTCGAACGTGATATAAATATTGTGAAGCACCAAATGGATCTCTTCTTGGTGGAGTATATTCCGGAAGCTTTGGATCTTATATCTCGATTGTTAAATCGTGACCCTGAATTGAGGCCAAAGGCTTTGGAGGTGTTGCACCATCCTCTTTTCTGGAGCTCTGAGTTGAGACTCTCATTCCTGCGAGACTATAGTGACAGGGTAGAACTGGAAGATAGACAGGCTAACTCTGATCTTCTGAAAGCCCTAGAAAGTACTGCGCCCGTGGCTTTGGGTGGGAAATGGAATGAAAAGATGGAGCCTGCATTCCTCACTAACATTGGCCATTACAGGCGTTATAAGTTTGACAGTGTTCGAGACTTACTGCGAGTCATGCGGAACAAATATAATCACTATAGAGAGCTCCCAAAGGAAATCCAGGAACTCCTAGGTCCAGTGCCCGAAGGATATGATCACTACTATGCAAGTCGGTTCCCAAAACTTCTGATTGAAGTATACAAAGTTGCGTGCAAACATTGTAGAGAAGAGGAATGGTTTCAGAAGTACTTCAAAAGCACTGCCAATCCCTCGGGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

853

Amino Acids

96.22

Weight (kDa)

8.37

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 432 - 705 5.5e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 433 - 711 3.8e-34 Protein kinase domain
Ribonuc_2-5A PF06479 717 - 843 7.5e-46 Ribonuclease 2-5A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2319
Acc36I ACCTGC 1 cut(s) 405
AccB1I GGYRCC 3 cut(s) 214, 283, 1322
AccI GTMKAC 3 cut(s) 1354, 1963, 2475
AccII CGCG 1 cut(s) 110
AccIII TCCGGA 1 cut(s) 2044
AciI CCGC 9 cut(s) 27, 93, 108, 185, 212, 287, 835, 1897, 2356
AclI AACGTT 1 cut(s) 1635
AclWI GGATC 8 cut(s) 460, 475, 491, 602, 901, 911, 2028, 2064
AcoI YGGCCR 1 cut(s) 2302
AcsI RAATTY 5 cut(s) 226, 516, 703, 1410, 1536
AcuI CTGAAG 2 cut(s) 98, 801
AcvI CACGTG 1 cut(s) 1907
AcyI GRCGYC 2 cut(s) 284, 431
AfaI GTAC 7 cut(s) 196, 293, 563, 1452, 1472, 2236, 2525
AfiI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
AflIII ACRYGT 3 cut(s) 1171, 1836, 1904
AgsI TTSAA 9 cut(s) 667, 687, 860, 927, 1092, 1592, 1681, 2471, 2530
AjiI CACGTC 1 cut(s) 1396
AjnI CCWGG 5 cut(s) 32, 303, 565, 1332, 2397
AluBI AGCT 9 cut(s) 1075, 1196, 1385, 1568, 1798, 1885, 2051, 2139, 2383
AluI AGCT 9 cut(s) 1075, 1196, 1385, 1568, 1798, 1885, 2051, 2139, 2383
Alw21I GWGCWC 2 cut(s) 2141, 2385
Alw26I GTCTC 4 cut(s) 700, 2143, 2160, 2331
AlwI GGATC 8 cut(s) 460, 475, 491, 602, 901, 911, 2028, 2064
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 2548
Aor13HI TCCGGA 1 cut(s) 2044
AoxI GGCC 5 cut(s) 142, 768, 2096, 2302, 2551
ApeKI GCWGC 3 cut(s) 124, 623, 1256
ApoI RAATTY 5 cut(s) 226, 516, 703, 1410, 1536
AseI ATTAAT 1 cut(s) 1769
AsiSI GCGATCGC 1 cut(s) 122
Asp700I GAANNNNTTC 3 cut(s) 1884, 2514, 2525
AspA2I CCTAGG 1 cut(s) 2407
AspLEI GCGC 4 cut(s) 112, 257, 286, 2242
AspS9I GGNCC 3 cut(s) 768, 2411, 2551
AsuHPI GGTGA 6 cut(s) 661, 1166, 1226, 1583, 1973, 1988
AvaI CYCGRG 1 cut(s) 2548
AvaII GGWCC 1 cut(s) 2411
AvrII CCTAGG 1 cut(s) 2407
BaeGI GKGCMC 1 cut(s) 2421
BalI TGGCCA 1 cut(s) 2304
BanI GGYRCC 3 cut(s) 214, 283, 1322
BanII GRGCYC 2 cut(s) 2141, 2385
BauI CACGAG 2 cut(s) 537, 990
BbrPI CACGTG 1 cut(s) 1907
BbsI GAAGAC 2 cut(s) 482, 1833
Bbv12I GWGCWC 2 cut(s) 2141, 2385
BbvI GCAGC 3 cut(s) 136, 610, 1243
BccI CCATC 8 cut(s) 186, 350, 443, 846, 871, 1449, 2130, 2269
BceAI ACGGC 1 cut(s) 80
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BciT130I CCWGG 5 cut(s) 34, 305, 567, 1334, 2399
BclI TGATCA 2 cut(s) 1978, 2431
BcoDI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BfaI CTAG 6 cut(s) 867, 1143, 1382, 2228, 2408, 2557
BfmI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 405
BisI GCNGC 7 cut(s) 27, 93, 125, 287, 624, 1257, 1898
BlnI CCTAGG 1 cut(s) 2407
BlsI GCNGC 7 cut(s) 28, 94, 126, 288, 625, 1258, 1899
BmcAI AGTACT 2 cut(s) 2236, 2525
Bme1390I CCNGG 5 cut(s) 34, 305, 567, 1334, 2399
Bme18I GGWCC 1 cut(s) 2411
BmeT110I CYCGRG 1 cut(s) 2548
BmgBI CACGTC 1 cut(s) 1396
BmgT120I GGNCC 3 cut(s) 768, 2411, 2551
BmiI GGNNCC 9 cut(s) 76, 216, 285, 309, 335, 769, 1324, 2280, 2453
BmrFI CCNGG 5 cut(s) 34, 305, 567, 1334, 2399
BmsI GCATC 3 cut(s) 1436, 1821, 1959
BpiI GAAGAC 2 cut(s) 482, 1833
BplI GAGNNNNNCTC 2 cut(s) 937, 969
BpmI CTGGAG 3 cut(s) 1355, 1520, 2155
Bpu10I CCTNAGC 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 297, 1528
Bsa29I ATCGAT 1 cut(s) 854
BsaAI YACGTR 2 cut(s) 340, 1907
BsaHI GRCGYC 2 cut(s) 284, 431
BsaJI CCNNGG 6 cut(s) 70, 565, 1308, 2244, 2407, 2547
BsaWI WCCGGW 4 cut(s) 506, 757, 1550, 2044
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
Bse1I ACTGG 3 cut(s) 1960, 2193, 2414
Bse3DI GCAATG 3 cut(s) 596, 939, 1324
BseAI TCCGGA 1 cut(s) 2044
BseBI CCWGG 5 cut(s) 34, 305, 567, 1334, 2399
BseCI ATCGAT 1 cut(s) 854
BseDI CCNNGG 6 cut(s) 70, 565, 1308, 2244, 2407, 2547
BseGI GGATG 8 cut(s) 66, 178, 1020, 1192, 1528, 1699, 1966, 2122
BseLI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
BseMI GCAATG 3 cut(s) 596, 939, 1324
BseMII CTCAG 5 cut(s) 927, 1090, 1196, 1200, 2133
BseNI ACTGG 3 cut(s) 1960, 2193, 2414
BseRI GAGGAG 8 cut(s) 41, 44, 47, 50, 53, 161, 300, 560
BseSI GKGCMC 1 cut(s) 2421
BseXI GCAGC 3 cut(s) 136, 610, 1243
BseYI CCCAGC 1 cut(s) 158
BsgI GTGCAG 2 cut(s) 1083, 1929
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 2 cut(s) 122, 1267
BshFI GGCC 5 cut(s) 144, 770, 2098, 2304, 2553
BshNI GGYRCC 3 cut(s) 214, 283, 1322
BshVI ATCGAT 1 cut(s) 854
BsiEI CGRYCG 2 cut(s) 122, 1267
BsiHKAI GWGCWC 2 cut(s) 2141, 2385
BsiHKCI CYCGRG 1 cut(s) 2548
BsiSI CCGG 6 cut(s) 218, 252, 507, 758, 1551, 2045
BslFI GGGAC 3 cut(s) 1651, 1757, 1847
BslI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
BsmAI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BsmFI GGGAC 3 cut(s) 1651, 1757, 1847
BsmI GAATGC 2 cut(s) 1144, 2285
BsnI GGCC 5 cut(s) 144, 770, 2098, 2304, 2553
BsoBI CYCGRG 1 cut(s) 2548
Bsp1286I GDGCHC 3 cut(s) 2141, 2385, 2421
Bsp13I TCCGGA 1 cut(s) 2044
Bsp1407I TGTACA 1 cut(s) 291
BspACI CCGC 9 cut(s) 27, 93, 108, 185, 212, 287, 835, 1897, 2356
BspANI GGCC 5 cut(s) 144, 770, 2098, 2304, 2553
BspCNI CTCAG 5 cut(s) 926, 1089, 1195, 1201, 2134
BspDI ATCGAT 1 cut(s) 854
BspEI TCCGGA 1 cut(s) 2044
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 9 cut(s) 76, 216, 285, 309, 335, 769, 1324, 2280, 2453
BspMAI CTGCAG 1 cut(s) 1261
BspMI ACCTGC 1 cut(s) 405
BspPI GGATC 8 cut(s) 460, 475, 491, 602, 901, 911, 2028, 2064
BspT107I GGYRCC 3 cut(s) 214, 283, 1322
BsrDI GCAATG 3 cut(s) 596, 939, 1324
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 3 cut(s) 1960, 2193, 2414
BssECI CCNNGG 6 cut(s) 70, 565, 1308, 2244, 2407, 2547
BssNAI GTATAC 1 cut(s) 2476
BssNI GRCGYC 2 cut(s) 284, 431
BssSI CACGAG 2 cut(s) 537, 990
BssT1I CCWWGG 2 cut(s) 1308, 2407
Bst1107I GTATAC 1 cut(s) 2476
Bst2BI CACGAG 2 cut(s) 537, 990
Bst2UI CCWGG 5 cut(s) 34, 305, 567, 1334, 2399
Bst4CI ACNGT 5 cut(s) 234, 296, 807, 1615, 2330
Bst6I CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
BstACI GRCGYC 2 cut(s) 284, 431
BstAUI TGTACA 1 cut(s) 291
BstBAI YACGTR 2 cut(s) 340, 1907
BstC8I GCNNGC 6 cut(s) 142, 787, 1820, 1872, 2283, 2488
BstDEI CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
BstDSI CCRYGG 1 cut(s) 2244
BstF5I GGATG 8 cut(s) 66, 178, 1020, 1192, 1528, 1699, 1966, 2122
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 4 cut(s) 112, 257, 286, 2242
BstMAI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BstMCI CGRYCG 2 cut(s) 122, 1267
BstMWI GCNNNNNNNGC 7 cut(s) 89, 109, 124, 150, 159, 2246, 2353
BstNI CCWGG 5 cut(s) 34, 305, 567, 1334, 2399
BstNSI RCATGY 4 cut(s) 941, 967, 1175, 1840
BstSCI CCNGG 5 cut(s) 32, 303, 565, 1332, 2397
BstSFI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
BstSLI GKGCMC 1 cut(s) 2421
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 3 cut(s) 136, 610, 1243
BstV2I GAAGAC 2 cut(s) 482, 1833
BstX2I RGATCY 4 cut(s) 906, 916, 2020, 2056
BstXI CCANNNNNNTGG 2 cut(s) 140, 1333
BstYI RGATCY 4 cut(s) 906, 916, 2020, 2056
BstZ17I GTATAC 1 cut(s) 2476
Bsu15I ATCGAT 1 cut(s) 854
BsuRI GGCC 5 cut(s) 144, 770, 2098, 2304, 2553
BsuTUI ATCGAT 1 cut(s) 854
BtgI CCRYGG 1 cut(s) 2244
BtrI CACGTC 1 cut(s) 1396
BtsCI GGATG 8 cut(s) 66, 178, 1020, 1192, 1528, 1699, 1966, 2122
BtsI GCAGTG 3 cut(s) 796, 1917, 2535
BtsIMutI CAGTG 7 cut(s) 243, 796, 1917, 1953, 2335, 2421, 2535
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 6 cut(s) 142, 787, 1820, 1872, 2283, 2488
CaiI CAGNNNCTG 1 cut(s) 397
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 4 cut(s) 112, 257, 286, 2242
Cfr13I GGNCC 3 cut(s) 768, 2411, 2551
ClaI ATCGAT 1 cut(s) 854
CseI GACGC 1 cut(s) 439
Csp6I GTAC 7 cut(s) 195, 292, 562, 1451, 1471, 2235, 2524
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 469, 504
CviQI GTAC 7 cut(s) 195, 292, 562, 1451, 1471, 2235, 2524
DdeI CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
DinI GGCGCC 1 cut(s) 285
DraI TTTAAA 1 cut(s) 1405
EaeI YGGCCR 1 cut(s) 2302
Eam1104I CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
EarI CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
EciI GGCGGA 1 cut(s) 824
Ecl136II GAGCTC 2 cut(s) 2139, 2383
Eco130I CCWWGG 2 cut(s) 1308, 2407
Eco24I GRGCYC 2 cut(s) 2141, 2385
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 2411
Eco53kI GAGCTC 2 cut(s) 2139, 2383
Eco57I CTGAAG 2 cut(s) 98, 801
Eco72I CACGTG 1 cut(s) 1907
Eco88I CYCGRG 1 cut(s) 2548
EcoICRI GAGCTC 2 cut(s) 2139, 2383
EcoO109I RGGNCCY 1 cut(s) 768
EcoRI GAATTC 1 cut(s) 516
EcoRII CCWGG 5 cut(s) 32, 303, 565, 1332, 2397
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 2 cut(s) 1308, 2407
EcoT22I ATGCAT 1 cut(s) 939
EcoT38I GRGCYC 2 cut(s) 2141, 2385
EgeI GGCGCC 1 cut(s) 285
EheI GGCGCC 1 cut(s) 285
ErhI CCWWGG 2 cut(s) 1308, 2407
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FaqI GGGAC 3 cut(s) 1651, 1757, 1847
FbaI TGATCA 2 cut(s) 1978, 2431
FblI GTMKAC 3 cut(s) 1354, 1963, 2475
Fnu4HI GCNGC 7 cut(s) 27, 93, 125, 287, 624, 1257, 1898
FokI GGATG 8 cut(s) 53, 165, 1007, 1199, 1535, 1706, 1953, 2109
FriOI GRGCYC 2 cut(s) 2141, 2385
Fsp4HI GCNGC 7 cut(s) 27, 93, 125, 287, 624, 1257, 1898
FspBI CTAG 6 cut(s) 867, 1143, 1382, 2228, 2408, 2557
GlaI GCGC 4 cut(s) 111, 256, 285, 2241
GluI GCNGC 7 cut(s) 27, 93, 125, 287, 624, 1257, 1898
GsaI CCCAGC 1 cut(s) 162
GsuI CTGGAG 3 cut(s) 1355, 1520, 2155
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 5 cut(s) 144, 770, 2098, 2304, 2553
HapII CCGG 6 cut(s) 218, 252, 507, 758, 1551, 2045
HgaI GACGC 1 cut(s) 439
HhaI GCGC 4 cut(s) 112, 257, 286, 2242
Hin1I GRCGYC 2 cut(s) 284, 431
Hin6I GCGC 4 cut(s) 110, 255, 284, 2240
HinP1I GCGC 4 cut(s) 110, 255, 284, 2240
HincII GTYRAC 2 cut(s) 1355, 1611
HindII GTYRAC 2 cut(s) 1355, 1611
HindIII AAGCTT 3 cut(s) 1194, 1796, 2049
HpaII CCGG 6 cut(s) 218, 252, 507, 758, 1551, 2045
HphI GGTGA 6 cut(s) 661, 1166, 1226, 1583, 1973, 1988
Hpy166II GTNNAC 7 cut(s) 241, 424, 1280, 1355, 1611, 1964, 2476
Hpy8I GTNNAC 7 cut(s) 241, 424, 1280, 1355, 1611, 1964, 2476
HpyAV CCTTC 7 cut(s) 53, 504, 661, 1107, 1113, 1343, 2417
HpyCH4III ACNGT 5 cut(s) 234, 296, 807, 1615, 2330
HpyCH4IV ACGT 5 cut(s) 339, 1395, 1635, 1906, 1990
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 109, 124, 150, 159, 2246, 2353
HpyF3I CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
HpySE526I ACGT 5 cut(s) 339, 1395, 1635, 1906, 1990
Hsp92I GRCGYC 2 cut(s) 284, 431
HspAI GCGC 4 cut(s) 110, 255, 284, 2240
KasI GGCGCC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 2044
Ksp22I TGATCA 2 cut(s) 1978, 2431
LmnI GCTCC 7 cut(s) 80, 313, 547, 1501, 2136, 2278, 2388
Lsp1109I GCAGC 3 cut(s) 136, 610, 1243
LweI GCATC 3 cut(s) 1436, 1821, 1959
MaeI CTAG 6 cut(s) 867, 1143, 1382, 2228, 2408, 2557
MaeII ACGT 5 cut(s) 339, 1395, 1635, 1906, 1990
MaeIII GTNAC 6 cut(s) 457, 649, 791, 987, 2081, 2174
MflI RGATCY 4 cut(s) 906, 916, 2020, 2056
MhlI GDGCHC 3 cut(s) 2141, 2385, 2421
MlsI TGGCCA 1 cut(s) 2304
MluNI TGGCCA 1 cut(s) 2304
Mly113I GGCGCC 1 cut(s) 284
MlyI GAGTC 6 cut(s) 763, 840, 994, 1129, 2145, 2358
MmeI TCCRAC 2 cut(s) 632, 1103
Mox20I TGGCCA 1 cut(s) 2304
Mph1103I ATGCAT 1 cut(s) 939
MroI TCCGGA 1 cut(s) 2044
MroXI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
MscI TGGCCA 1 cut(s) 2304
MseI TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
MslI CAYNNNNRTG 4 cut(s) 342, 687, 1719, 2439
Msp20I TGGCCA 1 cut(s) 2304
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 6 cut(s) 218, 252, 507, 758, 1551, 2045
MspR9I CCNGG 5 cut(s) 34, 305, 567, 1334, 2399
Mva1269I GAATGC 2 cut(s) 1144, 2285
MvaI CCWGG 5 cut(s) 34, 305, 567, 1334, 2399
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 7 cut(s) 89, 109, 124, 150, 159, 2246, 2353
NarI GGCGCC 1 cut(s) 284
NlaIV GGNNCC 9 cut(s) 76, 216, 285, 309, 335, 769, 1324, 2280, 2453
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 5 cut(s) 649, 791, 987, 2081, 2174
NsiI ATGCAT 1 cut(s) 939
NspI RCATGY 4 cut(s) 941, 967, 1175, 1840
PacI TTAATTAA 1 cut(s) 1773
PagI TCATGA 1 cut(s) 1390
PciI ACATGT 2 cut(s) 1171, 1836
PctI GAATGC 2 cut(s) 1144, 2285
PdmI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
PfeI GAWTC 5 cut(s) 248, 1088, 1417, 1588, 1621
PfoI TCCNGGA 2 cut(s) 1332, 2397
PkrI GCNGC 7 cut(s) 28, 94, 126, 288, 625, 1258, 1899
Ple19I CGATCG 1 cut(s) 122
PleI GAGTC 6 cut(s) 762, 839, 994, 1128, 2145, 2357
PluTI GGCGCC 1 cut(s) 287
PmaCI CACGTG 1 cut(s) 1907
PmlI CACGTG 1 cut(s) 1907
PpsI GAGTC 6 cut(s) 762, 839, 994, 1128, 2145, 2357
Ppu21I YACGTR 2 cut(s) 340, 1907
PscI ACATGT 2 cut(s) 1171, 1836
PshBI ATTAAT 1 cut(s) 1769
PsiI TTATAA 1 cut(s) 2319
Psp124BI GAGCTC 2 cut(s) 2141, 2385
Psp1406I AACGTT 1 cut(s) 1635
Psp6I CCWGG 5 cut(s) 32, 303, 565, 1332, 2397
PspCI CACGTG 1 cut(s) 1907
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 5 cut(s) 32, 303, 565, 1332, 2397
PspN4I GGNNCC 9 cut(s) 76, 216, 285, 309, 335, 769, 1324, 2280, 2453
PspPI GGNCC 3 cut(s) 768, 2411, 2551
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 4 cut(s) 906, 916, 2020, 2056
PvuI CGATCG 1 cut(s) 122
RgaI GCGATCGC 1 cut(s) 122
RsaI GTAC 7 cut(s) 196, 293, 563, 1452, 1472, 2236, 2525
RsaNI GTAC 7 cut(s) 195, 292, 562, 1451, 1471, 2235, 2524
RseI CAYNNNNRTG 4 cut(s) 342, 687, 1719, 2439
SacI GAGCTC 2 cut(s) 2141, 2385
SalI GTCGAC 1 cut(s) 1353
SaqAI TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
SatI GCNGC 7 cut(s) 27, 93, 125, 287, 624, 1257, 1898
Sau96I GGNCC 3 cut(s) 768, 2411, 2551
ScaI AGTACT 2 cut(s) 2236, 2525
SchI GAGTC 6 cut(s) 763, 840, 994, 1129, 2145, 2358
ScrFI CCNGG 5 cut(s) 34, 305, 567, 1334, 2399
SduI GDGCHC 3 cut(s) 2141, 2385, 2421
SfaAI GCGATCGC 1 cut(s) 122
SfaNI GCATC 3 cut(s) 1436, 1821, 1959
SfcI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
SfoI GGCGCC 1 cut(s) 285
SgfI GCGATCGC 1 cut(s) 122
SinI GGWCC 1 cut(s) 2411
SmiMI CAYNNNNRTG 4 cut(s) 342, 687, 1719, 2439
SmlI CTYRAG 2 cut(s) 312, 1543
SmoI CTYRAG 2 cut(s) 312, 1543
SsiI CCGC 9 cut(s) 27, 93, 108, 185, 212, 287, 835, 1897, 2356
SspDI GGCGCC 1 cut(s) 283
SspI AATATT 1 cut(s) 2002
SspMI CTAG 6 cut(s) 867, 1143, 1382, 2228, 2408, 2557
SstI GAGCTC 2 cut(s) 2141, 2385
StyD4I CCNGG 5 cut(s) 32, 303, 565, 1332, 2397
StyI CCWWGG 2 cut(s) 1308, 2407
TaaI ACNGT 5 cut(s) 234, 296, 807, 1615, 2330
TaiI ACGT 5 cut(s) 342, 1398, 1638, 1909, 1993
TaqI TCGA 6 cut(s) 470, 854, 1354, 1986, 2068, 2335
TaqII GACCGA 1 cut(s) 1253
TatI WGTACW 3 cut(s) 291, 2234, 2523
TauI GCSGC 4 cut(s) 29, 95, 289, 1900
TfiI GAWTC 5 cut(s) 248, 1088, 1417, 1588, 1621
Tru1I TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
Tru9I TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
TscAI CASTG 7 cut(s) 250, 796, 1917, 1960, 2335, 2421, 2542
TseFI GTSAC 5 cut(s) 649, 791, 987, 2081, 2174
TseI GCWGC 3 cut(s) 124, 623, 1256
Tsp45I GTSAC 5 cut(s) 649, 791, 987, 2081, 2174
TspRI CASTG 7 cut(s) 250, 796, 1917, 1960, 2335, 2421, 2542
VpaK11BI GGWCC 1 cut(s) 2411
VspI ATTAAT 1 cut(s) 1769
XapI RAATTY 5 cut(s) 226, 516, 703, 1410, 1536
XbaI TCTAGA 1 cut(s) 866
XceI RCATGY 4 cut(s) 941, 967, 1175, 1840
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmaJI CCTAGG 1 cut(s) 2407
XmiI GTMKAC 3 cut(s) 1354, 1963, 2475
XmnI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
XspI CTAG 6 cut(s) 867, 1143, 1382, 2228, 2408, 2557
ZrmI AGTACT 2 cut(s) 2236, 2525
Zsp2I ATGCAT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.