Rroxscaffold_2G00145110

Serine threonine-protein kinase endoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011468
Physical Location & Seq
Reverse (-)
82973869 .. 82978072
4204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145110.1

Sequence Viewer

Length: 2562 bp
ATGAAGCACCACCACCAGCACCATGCCGCCACATGGAAACTCCTACTCCTCCTCCTCCTCCTCCTCATCCTCGGCTCCACAGGCCCAAACGCCGCCGTTTCGGCTTCCGCGCTTCTGCGATCGCAGCCGAACCATGACGCCGGCCTCTCTCGCTCTCCTAGCCGATCGCTCCTCTCCCATCCACCGCCTAAGCGTAGCACTCAATTTGTGGCGTTGCCGGAGGGGAAGTTACGGTTAGTGGACACTGATTCCGGTGCAGTAATTTATACTTTGGATTTAGGGGCGCCGCTGTACAGTTCATACCAGGCTCCTCAAGGCAAAGATAACAATAGGTTCCACGTAGATGTTGATGAAGATGGCGACGTCTATTTGGTCAGTAATGATATCAAACAGAACCTGCATATTCCAATGGCAGAGTTTGTCCACAAGACGCCTTACACATTAGAGGATGGATCAGTTACACTTGGATCGAAGAAGACCACTACCTTTGTGATTGATCCGATGACCGGAAGGACAATTCACACATACACTTCATCTCGTGGCGAGGAGCAGGGTGTTCTGTACCCTGGTTTTATTAAAGGCATTAAGCAATGGATCAAGCCTGTTCCAACCAATCCAAATGCTGCTAAGTCGCTACTATATGTCTTGGTGACAGAGTATTCCTTGAAGGCATACTTTCCACATTCAAATGAAGTCTCATGGAATTTGACATATTCTGAAATTGGAGTTATTTCATTTTGTTCAGATATTGAGAGTCCGGTTAGTGGGGCCTTTGGTTCTGAAGCAGGCAGTGATATTGTTTTACCATTATCATGTCAATCAAAAATAAGAGTCCGCCATCAAAATCGTATATCGATTGAACCATCTAGACATGGCAGATTGTTAGAGGAACATAACACAGATAAGATCCTCTCAGATCCCACTTCAAATGCAATGCATGTTGTGAGAAAATCTCCTAAAGGCATGTTAGTCATGCTTTTTGATTGGTCACGAGTTTTGACTCTCACTTTCTCCTTTGTCATCCTCATGGGCTTTGTTATATACTGCTATACTTATGTAGTTAAAAGGCAACAGCTCAGTAATTCTGATTCAAATACTGCACCTTCCAAGAGAAGGAAGAGTAGGAAATCAGAAAAGAATGCTAGTGGCATTGGTGAAGAAAAGGAGAAACATGTATCATCTCAGGATGAGGAAGCTTTAGCACACTCTGAGGGTGATAACAAAATGTTGTTCCTTGATAAACTATTTTATGGTGCTACAGTTGGTCGCAGGATTGGTAAACTACTTGTATCAAATACAGAGATCGCCAAGGGAAGCAATGGCACCATTGTCCTGGAGGGAGTTTATGAAGGTCGACCTGTTGCAGTCAAACGCCTTGTTCTAGCTCATCATGATGTGGCTTTTAAAGAAATTCAGAATCTTATCGCATCTGACCAACATCAGAACATAGTCCGATGGTACGGCGTGGAGTACGATCGAGATTTTGTCTATCTCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTACTCAAATTCTGGTGAAAATCCTGTAGCTGGTGAGGAATATGCTATGATTCAAAATAAAGTTCGCTTGTCAACAGTAAAGAATCTTATGCCGAACGTTAATTTGTGGAAAGAAAATGGTCTATTGTCCCCCCTATTGTTGAAATTGATGAGGGATGTCGTCTGTGGGCTTGTGCATTTACATGAATTGGGGATAATTCATAGGGACTTAAAGCCTCAAAATGTTTTGTTAATTAAGGAAAGTTCATTTTGTGCAAAGCTTTCTGACATGGGCATCAGCAAGCGACTTATTGGGGACATGTCTTCTTTGGGTTCTGGTAGTTCGGGTTGGCAAGCACCTGAACAGCTTCTTCATGGGCGACAAACACGTGCAGTGGATTTGTTTAGTTTAGGTTGTGTCCTCTTCTTTTGCATCACTGGTGGTAGACATCCATTTGGTGATCGCCTCGAACGTGATATAAATATTGTGAAGAACCAAATGGATCTCTTCTTGGTGGAGTATATTCCGGAAGCTTTGGATCTTATATCTCGATTGTTAAATCGTGACCCTGAATTGAGGCCAAAGGCTTTGGAGGTGTTGCACCATCCTCTTTTCTGGAGCTCTGAGTTGAGACTCTCATTCCTGCGAGACTATAGTGACAGGGTAGAACTGGAAGATAGACAGGCTAACTCTGATCTTCTGAAAGCCCTAGAAAGTACCGCGCCTGTGGCTTTGGGTGGGAAATGGAATGAAAAGATGGATCCTGCATTCCTCACTAACATTGGCCATTACAGGCGATATAAGTTTGACAGTGTTCGAGACTTACTGCGAGTCATGCGGAACAAATATAATCACTATAGAGAGCTCCCAAAGGAAATCCAGGAACTCCTAGGTCAAGTGCCCGAAGGATATGATCACTACTTTGCAAGTCGGTTCCCAAAACTTCTGATTGAAGTATACAAAGTTGCGTGCAAACATTGTAGAGAAGAGGAATGGTTTCAGAAGTATTTCAAAAGCACTGCCAATGAGTCCTCGGGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005975 GO:0006020 GO:0006066 GO:0006139 GO:0006396 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006986 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010467 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0019538 GO:0019751 GO:0019898 GO:0022607 GO:0023052 GO:0030176 GO:0030968 GO:0031090 GO:0031224 GO:0031227 GO:0031312 GO:0031505 GO:0031984 GO:0033036 GO:0033365 GO:0033554 GO:0034067 GO:0034613 GO:0034620 GO:0034641 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036290 GO:0042175 GO:0042221 GO:0042406 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046677 GO:0046777 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051179 GO:0051259 GO:0051260 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0098542 GO:0098827 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1901615 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010
Pfam Domains
Protein Families
Pkinase Ribonuc_2-5A

Protein Analysis

853

Amino Acids

96.36

Weight (kDa)

8.27

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 432 - 705 9.2e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 433 - 711 7.6e-34 Protein kinase domain
Ribonuc_2-5A PF06479 717 - 843 9.6e-47 Ribonuclease 2-5A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17520
fragaria_vesca FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190 FvH4_1g10190
malus_domestica MD02G1114000.v1.1
prunus_persica Prupe.7G185000_v2.0.a1
pyrus_communis pycom02g08880
rosa_chinensis RchiOBHm_Chr2g0096971
rosa_laevigata RLG00000016688
rosa_multiflora Rmu_sc0001651.1_g000010
rosa_roxburghii Rroxscaffold_2G00145110
rosa_rugosa Rorug02G0062600 Rorug02G0062600
rosa_samantha Rh2AG110400 Rh2BG112200 Rh2CG114300 Rh2DG113400
rosa_wichuraiana Rw2G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 405
AccB1I GGYRCC 2 cut(s) 283, 1322
AccI GTMKAC 3 cut(s) 1354, 1963, 2475
AccII CGCG 2 cut(s) 110, 2240
AccIII TCCGGA 1 cut(s) 2044
AciI CCGC 8 cut(s) 27, 93, 108, 185, 287, 835, 2238, 2356
AclI AACGTT 1 cut(s) 1635
AcoI YGGCCR 1 cut(s) 2302
AcsI RAATTY 4 cut(s) 703, 1410, 1536, 1546
AcuI CTGAAG 1 cut(s) 801
AcvI CACGTG 1 cut(s) 1907
AcyI GRCGYC 4 cut(s) 138, 284, 363, 431
AfaI GTAC 5 cut(s) 293, 563, 1460, 1472, 2236
AfiI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
AflIII ACRYGT 3 cut(s) 1171, 1836, 1904
AgsI TTSAA 9 cut(s) 667, 687, 860, 927, 1092, 1592, 1681, 2471, 2530
AjnI CCWGG 4 cut(s) 303, 565, 1332, 2397
AluBI AGCT 9 cut(s) 1075, 1196, 1385, 1568, 1798, 1885, 2051, 2139, 2383
AluI AGCT 9 cut(s) 1075, 1196, 1385, 1568, 1798, 1885, 2051, 2139, 2383
Alw21I GWGCWC 2 cut(s) 2141, 2385
Alw26I GTCTC 4 cut(s) 700, 2143, 2160, 2331
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 2551
Aor13HI TCCGGA 1 cut(s) 2044
AoxI GGCC 5 cut(s) 82, 142, 768, 2096, 2302
ApeKI GCWGC 2 cut(s) 124, 623
ApoI RAATTY 4 cut(s) 703, 1410, 1536, 1546
AsiSI GCGATCGC 1 cut(s) 122
Asp700I GAANNNNTTC 3 cut(s) 1884, 2514, 2525
AspA2I CCTAGG 1 cut(s) 2407
AspLEI GCGC 3 cut(s) 112, 286, 2242
AspS9I GGNCC 2 cut(s) 83, 768
AsuHPI GGTGA 6 cut(s) 661, 1166, 1226, 1565, 1583, 1988
AvaI CYCGRG 1 cut(s) 2551
AvrII CCTAGG 1 cut(s) 2407
BaeGI GKGCMC 1 cut(s) 2421
BalI TGGCCA 1 cut(s) 2304
BamHI GGATCC 1 cut(s) 2278
BanI GGYRCC 2 cut(s) 283, 1322
BanII GRGCYC 2 cut(s) 2141, 2385
BauI CACGAG 2 cut(s) 537, 990
BbrPI CACGTG 1 cut(s) 1907
BbsI GAAGAC 2 cut(s) 482, 1833
Bbv12I GWGCWC 2 cut(s) 2141, 2385
BbvI GCAGC 2 cut(s) 136, 610
BccI CCATC 8 cut(s) 186, 350, 443, 846, 871, 1449, 2130, 2269
BceAI ACGGC 2 cut(s) 80, 1477
BciT130I CCWGG 4 cut(s) 305, 567, 1334, 2399
BclI TGATCA 1 cut(s) 2431
BcoDI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BfaI CTAG 7 cut(s) 159, 867, 1143, 1382, 2228, 2408, 2560
BfmI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 5 cut(s) 27, 93, 125, 287, 624
BlnI CCTAGG 1 cut(s) 2407
BlsI GCNGC 5 cut(s) 28, 94, 126, 288, 625
Bme1390I CCNGG 4 cut(s) 305, 567, 1334, 2399
BmeT110I CYCGRG 1 cut(s) 2551
BmgT120I GGNCC 2 cut(s) 83, 768
BmiI GGNNCC 8 cut(s) 76, 285, 309, 335, 769, 1324, 2280, 2453
BmrFI CCNGG 4 cut(s) 305, 567, 1334, 2399
BmsI GCATC 3 cut(s) 1436, 1821, 1959
BpiI GAAGAC 2 cut(s) 482, 1833
BplI GAGNNNNNCTC 2 cut(s) 937, 969
BpmI CTGGAG 3 cut(s) 1355, 1520, 2155
Bpu10I CCTNAGC 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 297
Bsa29I ATCGAT 1 cut(s) 854
BsaAI YACGTR 2 cut(s) 340, 1907
BsaHI GRCGYC 4 cut(s) 138, 284, 363, 431
BsaJI CCNNGG 5 cut(s) 70, 565, 1308, 2407, 2550
BsaWI WCCGGW 4 cut(s) 251, 506, 757, 2044
BsaXI ACNNNNNCTCC 6 cut(s) 30, 36, 60, 66, 212, 242
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 1960, 2193
Bse3DI GCAATG 3 cut(s) 596, 939, 1324
BseAI TCCGGA 1 cut(s) 2044
BseBI CCWGG 4 cut(s) 305, 567, 1334, 2399
BseCI ATCGAT 1 cut(s) 854
BseDI CCNNGG 5 cut(s) 70, 565, 1308, 2407, 2550
BseGI GGATG 9 cut(s) 66, 178, 454, 1020, 1192, 1528, 1699, 1966, 2122
BseLI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
BseMI GCAATG 3 cut(s) 596, 939, 1324
BseMII CTCAG 5 cut(s) 927, 1090, 1196, 1200, 2133
BseNI ACTGG 2 cut(s) 1960, 2193
BseRI GAGGAG 9 cut(s) 38, 41, 44, 47, 50, 53, 161, 300, 560
BseSI GKGCMC 1 cut(s) 2421
BseXI GCAGC 2 cut(s) 136, 610
BsgI GTGCAG 3 cut(s) 276, 1083, 1929
Bsh1236I CGCG 2 cut(s) 110, 2240
Bsh1285I CGRYCG 3 cut(s) 122, 167, 1477
BshFI GGCC 5 cut(s) 84, 144, 770, 2098, 2304
BshNI GGYRCC 2 cut(s) 283, 1322
BshVI ATCGAT 1 cut(s) 854
BsiEI CGRYCG 3 cut(s) 122, 167, 1477
BsiHKAI GWGCWC 2 cut(s) 2141, 2385
BsiHKCI CYCGRG 1 cut(s) 2551
BsiSI CCGG 6 cut(s) 141, 218, 252, 507, 758, 2045
BslFI GGGAC 3 cut(s) 1651, 1757, 1847
BslI CCNNNNNNNGG 5 cut(s) 33, 506, 764, 1113, 1568
BsmAI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BsmFI GGGAC 3 cut(s) 1651, 1757, 1847
BsmI GAATGC 2 cut(s) 1144, 2285
BsnI GGCC 5 cut(s) 84, 144, 770, 2098, 2304
BsoBI CYCGRG 1 cut(s) 2551
Bsp1286I GDGCHC 3 cut(s) 2141, 2385, 2421
Bsp13I TCCGGA 1 cut(s) 2044
Bsp1407I TGTACA 1 cut(s) 291
BspACI CCGC 8 cut(s) 27, 93, 108, 185, 287, 835, 2238, 2356
BspANI GGCC 5 cut(s) 84, 144, 770, 2098, 2304
BspCNI CTCAG 5 cut(s) 926, 1089, 1195, 1201, 2134
BspDI ATCGAT 1 cut(s) 854
BspEI TCCGGA 1 cut(s) 2044
BspFNI CGCG 2 cut(s) 110, 2240
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 8 cut(s) 76, 285, 309, 335, 769, 1324, 2280, 2453
BspMI ACCTGC 1 cut(s) 405
BspT107I GGYRCC 2 cut(s) 283, 1322
BsrDI GCAATG 3 cut(s) 596, 939, 1324
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 2 cut(s) 1960, 2193
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 5 cut(s) 70, 565, 1308, 2407, 2550
BssNAI GTATAC 1 cut(s) 2476
BssNI GRCGYC 4 cut(s) 138, 284, 363, 431
BssSI CACGAG 2 cut(s) 537, 990
BssT1I CCWWGG 2 cut(s) 1308, 2407
Bst1107I GTATAC 1 cut(s) 2476
Bst2BI CACGAG 2 cut(s) 537, 990
Bst2UI CCWGG 4 cut(s) 305, 567, 1334, 2399
Bst4CI ACNGT 5 cut(s) 234, 296, 1261, 1615, 2330
Bst6I CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
BstACI GRCGYC 4 cut(s) 138, 284, 363, 431
BstAUI TGTACA 1 cut(s) 291
BstBAI YACGTR 2 cut(s) 340, 1907
BstC8I GCNNGC 5 cut(s) 142, 787, 1820, 1872, 2488
BstDEI CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
BstF5I GGATG 9 cut(s) 66, 178, 454, 1020, 1192, 1528, 1699, 1966, 2122
BstFNI CGCG 2 cut(s) 110, 2240
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 3 cut(s) 112, 286, 2242
BstMAI GTCTC 4 cut(s) 700, 2143, 2160, 2331
BstMCI CGRYCG 3 cut(s) 122, 167, 1477
BstMWI GCNNNNNNNGC 7 cut(s) 81, 101, 124, 150, 159, 2246, 2353
BstNI CCWGG 4 cut(s) 305, 567, 1334, 2399
BstNSI RCATGY 4 cut(s) 941, 967, 1175, 1840
BstSCI CCNGG 4 cut(s) 303, 565, 1332, 2397
BstSFI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
BstSLI GKGCMC 1 cut(s) 2421
BstUI CGCG 2 cut(s) 110, 2240
BstV1I GCAGC 2 cut(s) 136, 610
BstV2I GAAGAC 2 cut(s) 482, 1833
BstX2I RGATCY 5 cut(s) 906, 916, 2020, 2056, 2278
BstXI CCANNNNNNTGG 1 cut(s) 1333
BstYI RGATCY 5 cut(s) 906, 916, 2020, 2056, 2278
BstZ17I GTATAC 1 cut(s) 2476
Bsu15I ATCGAT 1 cut(s) 854
BsuRI GGCC 5 cut(s) 84, 144, 770, 2098, 2304
BsuTUI ATCGAT 1 cut(s) 854
BtsCI GGATG 9 cut(s) 66, 178, 454, 1020, 1192, 1528, 1699, 1966, 2122
BtsI GCAGTG 3 cut(s) 796, 1917, 2535
BtsIMutI CAGTG 6 cut(s) 243, 796, 1917, 1953, 2335, 2535
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 5 cut(s) 142, 787, 1820, 1872, 2488
CaiI CAGNNNCTG 1 cut(s) 397
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 3 cut(s) 112, 286, 2242
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 83, 768
ClaI ATCGAT 1 cut(s) 854
CseI GACGC 2 cut(s) 146, 439
Csp6I GTAC 5 cut(s) 292, 562, 1459, 1471, 2235
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviQI GTAC 5 cut(s) 292, 562, 1459, 1471, 2235
DdeI CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
DinI GGCGCC 1 cut(s) 285
DraI TTTAAA 1 cut(s) 1405
EaeI YGGCCR 1 cut(s) 2302
Eam1104I CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
EarI CTCTTC 4 cut(s) 1112, 1946, 2030, 2499
EciI GGCGGA 1 cut(s) 824
Ecl136II GAGCTC 2 cut(s) 2139, 2383
Eco130I CCWWGG 2 cut(s) 1308, 2407
Eco24I GRGCYC 2 cut(s) 2141, 2385
Eco32I GATATC 1 cut(s) 385
Eco53kI GAGCTC 2 cut(s) 2139, 2383
Eco57I CTGAAG 1 cut(s) 801
Eco72I CACGTG 1 cut(s) 1907
Eco88I CYCGRG 1 cut(s) 2551
EcoICRI GAGCTC 2 cut(s) 2139, 2383
EcoO109I RGGNCCY 1 cut(s) 768
EcoRII CCWGG 4 cut(s) 303, 565, 1332, 2397
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 2 cut(s) 1308, 2407
EcoT22I ATGCAT 1 cut(s) 939
EcoT38I GRGCYC 2 cut(s) 2141, 2385
EgeI GGCGCC 1 cut(s) 285
EheI GGCGCC 1 cut(s) 285
ErhI CCWWGG 2 cut(s) 1308, 2407
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FaqI GGGAC 3 cut(s) 1651, 1757, 1847
FbaI TGATCA 1 cut(s) 2431
FblI GTMKAC 3 cut(s) 1354, 1963, 2475
Fnu4HI GCNGC 5 cut(s) 27, 93, 125, 287, 624
FokI GGATG 9 cut(s) 53, 165, 461, 1007, 1199, 1535, 1706, 1953, 2109
FriOI GRGCYC 2 cut(s) 2141, 2385
Fsp4HI GCNGC 5 cut(s) 27, 93, 125, 287, 624
FspBI CTAG 7 cut(s) 159, 867, 1143, 1382, 2228, 2408, 2560
GlaI GCGC 3 cut(s) 111, 285, 2241
GluI GCNGC 5 cut(s) 27, 93, 125, 287, 624
GsuI CTGGAG 3 cut(s) 1355, 1520, 2155
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 5 cut(s) 84, 144, 770, 2098, 2304
HapII CCGG 6 cut(s) 141, 218, 252, 507, 758, 2045
HgaI GACGC 2 cut(s) 146, 439
HhaI GCGC 3 cut(s) 112, 286, 2242
Hin1I GRCGYC 4 cut(s) 138, 284, 363, 431
Hin6I GCGC 3 cut(s) 110, 284, 2240
HinP1I GCGC 3 cut(s) 110, 284, 2240
HincII GTYRAC 2 cut(s) 1355, 1611
HindII GTYRAC 2 cut(s) 1355, 1611
HindIII AAGCTT 3 cut(s) 1194, 1796, 2049
HpaII CCGG 6 cut(s) 141, 218, 252, 507, 758, 2045
HphI GGTGA 6 cut(s) 661, 1166, 1226, 1565, 1583, 1988
Hpy166II GTNNAC 7 cut(s) 241, 424, 1280, 1355, 1611, 1964, 2476
Hpy8I GTNNAC 7 cut(s) 241, 424, 1280, 1355, 1611, 1964, 2476
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 6 cut(s) 504, 661, 1107, 1113, 1343, 2417
HpyCH4III ACNGT 5 cut(s) 234, 296, 1261, 1615, 2330
HpyCH4IV ACGT 5 cut(s) 339, 363, 1635, 1906, 1990
HpyF10VI GCNNNNNNNGC 7 cut(s) 81, 101, 124, 150, 159, 2246, 2353
HpyF3I CTNAG 7 cut(s) 189, 627, 913, 1076, 1182, 1209, 2142
HpySE526I ACGT 5 cut(s) 339, 363, 1635, 1906, 1990
Hsp92I GRCGYC 4 cut(s) 138, 284, 363, 431
HspAI GCGC 3 cut(s) 110, 284, 2240
KasI GGCGCC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 2044
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 2431
LmnI GCTCC 7 cut(s) 80, 174, 313, 547, 1501, 2136, 2388
Lsp1109I GCAGC 2 cut(s) 136, 610
LweI GCATC 3 cut(s) 1436, 1821, 1959
MaeI CTAG 7 cut(s) 159, 867, 1143, 1382, 2228, 2408, 2560
MaeII ACGT 5 cut(s) 339, 363, 1635, 1906, 1990
MaeIII GTNAC 6 cut(s) 228, 457, 649, 987, 2081, 2174
MflI RGATCY 5 cut(s) 906, 916, 2020, 2056, 2278
MhlI GDGCHC 3 cut(s) 2141, 2385, 2421
MlsI TGGCCA 1 cut(s) 2304
MluNI TGGCCA 1 cut(s) 2304
Mly113I GGCGCC 1 cut(s) 284
MlyI GAGTC 7 cut(s) 763, 840, 994, 2145, 2358, 2550, 2555
MmeI TCCRAC 1 cut(s) 632
Mox20I TGGCCA 1 cut(s) 2304
Mph1103I ATGCAT 1 cut(s) 939
MroI TCCGGA 1 cut(s) 2044
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
MscI TGGCCA 1 cut(s) 2304
MseI TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
MslI CAYNNNNRTG 6 cut(s) 342, 687, 811, 1025, 1392, 1719
Msp20I TGGCCA 1 cut(s) 2304
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 6 cut(s) 141, 218, 252, 507, 758, 2045
MspR9I CCNGG 4 cut(s) 305, 567, 1334, 2399
Mva1269I GAATGC 2 cut(s) 1144, 2285
MvaI CCWGG 4 cut(s) 305, 567, 1334, 2399
MvnI CGCG 2 cut(s) 110, 2240
MwoI GCNNNNNNNGC 7 cut(s) 81, 101, 124, 150, 159, 2246, 2353
NaeI GCCGGC 1 cut(s) 142
NarI GGCGCC 1 cut(s) 284
NgoMIV GCCGGC 1 cut(s) 140
NlaIV GGNNCC 8 cut(s) 76, 285, 309, 335, 769, 1324, 2280, 2453
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 4 cut(s) 649, 987, 2081, 2174
NsiI ATGCAT 1 cut(s) 939
NspI RCATGY 4 cut(s) 941, 967, 1175, 1840
PacI TTAATTAA 1 cut(s) 1773
PagI TCATGA 1 cut(s) 1390
PciI ACATGT 2 cut(s) 1171, 1836
PcsI WCGNNNNNNNCGW 1 cut(s) 1987
PctI GAATGC 2 cut(s) 1144, 2285
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
PfeI GAWTC 5 cut(s) 248, 1088, 1417, 1588, 1621
PfoI TCCNGGA 2 cut(s) 1332, 2397
PkrI GCNGC 5 cut(s) 28, 94, 126, 288, 625
Ple19I CGATCG 3 cut(s) 122, 167, 1477
PleI GAGTC 7 cut(s) 762, 839, 994, 2145, 2357, 2550, 2554
PluTI GGCGCC 1 cut(s) 287
PmaCI CACGTG 1 cut(s) 1907
PmlI CACGTG 1 cut(s) 1907
PpsI GAGTC 7 cut(s) 762, 839, 994, 2145, 2357, 2550, 2554
Ppu21I YACGTR 2 cut(s) 340, 1907
PscI ACATGT 2 cut(s) 1171, 1836
Psp124BI GAGCTC 2 cut(s) 2141, 2385
Psp1406I AACGTT 1 cut(s) 1635
Psp6I CCWGG 4 cut(s) 303, 565, 1332, 2397
PspCI CACGTG 1 cut(s) 1907
PspGI CCWGG 4 cut(s) 303, 565, 1332, 2397
PspN4I GGNNCC 8 cut(s) 76, 285, 309, 335, 769, 1324, 2280, 2453
PspPI GGNCC 2 cut(s) 83, 768
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 5 cut(s) 906, 916, 2020, 2056, 2278
PvuI CGATCG 3 cut(s) 122, 167, 1477
RgaI GCGATCGC 1 cut(s) 122
RsaI GTAC 5 cut(s) 293, 563, 1460, 1472, 2236
RsaNI GTAC 5 cut(s) 292, 562, 1459, 1471, 2235
RseI CAYNNNNRTG 6 cut(s) 342, 687, 811, 1025, 1392, 1719
SacI GAGCTC 2 cut(s) 2141, 2385
SalI GTCGAC 1 cut(s) 1353
SaqAI TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
SatI GCNGC 5 cut(s) 27, 93, 125, 287, 624
Sau96I GGNCC 2 cut(s) 83, 768
SchI GAGTC 7 cut(s) 763, 840, 994, 2145, 2358, 2550, 2555
ScrFI CCNGG 4 cut(s) 305, 567, 1334, 2399
SduI GDGCHC 3 cut(s) 2141, 2385, 2421
SfaAI GCGATCGC 1 cut(s) 122
SfaNI GCATC 3 cut(s) 1436, 1821, 1959
SfcI CTRYAG 4 cut(s) 1257, 1563, 2170, 2374
SfoI GGCGCC 1 cut(s) 285
SgfI GCGATCGC 1 cut(s) 122
SmiMI CAYNNNNRTG 6 cut(s) 342, 687, 811, 1025, 1392, 1719
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SsiI CCGC 8 cut(s) 27, 93, 108, 185, 287, 835, 2238, 2356
SspDI GGCGCC 1 cut(s) 283
SspI AATATT 1 cut(s) 2002
SspMI CTAG 7 cut(s) 159, 867, 1143, 1382, 2228, 2408, 2560
SstI GAGCTC 2 cut(s) 2141, 2385
StyD4I CCNGG 4 cut(s) 303, 565, 1332, 2397
StyI CCWWGG 2 cut(s) 1308, 2407
TaaI ACNGT 5 cut(s) 234, 296, 1261, 1615, 2330
TaiI ACGT 5 cut(s) 342, 366, 1638, 1909, 1993
TaqI TCGA 7 cut(s) 470, 854, 1354, 1477, 1986, 2068, 2335
TatI WGTACW 1 cut(s) 291
TauI GCSGC 3 cut(s) 29, 95, 289
TfiI GAWTC 5 cut(s) 248, 1088, 1417, 1588, 1621
Tru1I TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
Tru9I TTAA 9 cut(s) 576, 585, 1062, 1404, 1638, 1748, 1769, 1773, 2075
TscAI CASTG 6 cut(s) 250, 796, 1917, 1960, 2335, 2542
TseFI GTSAC 4 cut(s) 649, 987, 2081, 2174
TseI GCWGC 2 cut(s) 124, 623
Tsp45I GTSAC 4 cut(s) 649, 987, 2081, 2174
TspRI CASTG 6 cut(s) 250, 796, 1917, 1960, 2335, 2542
XapI RAATTY 4 cut(s) 703, 1410, 1536, 1546
XbaI TCTAGA 1 cut(s) 866
XceI RCATGY 4 cut(s) 941, 967, 1175, 1840
XmaJI CCTAGG 1 cut(s) 2407
XmiI GTMKAC 3 cut(s) 1354, 1963, 2475
XmnI GAANNNNTTC 3 cut(s) 1884, 2514, 2525
XspI CTAG 7 cut(s) 159, 867, 1143, 1382, 2228, 2408, 2560
ZraI GACGTC 1 cut(s) 364
Zsp2I ATGCAT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.