MD02G1122500.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010681
Physical Location & Seq
Reverse (-)
9964937 .. 9970543
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1122500.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGGCTATCGACGAGGACGTGGAGAGCTGCGGCAGCCGAGCGGTGCTGTCGTCGCAGGCGCAGCCTCGCCACCACCGGCAGAAGCTGGAGGTCTACAACGAGGTGCTCCGACGAATTCAAGAGTCCAATTTTGAGGAGGCGAATCTTCCTGGATTTGATGATCAGCTATGGCTTCATTTCAATCGGTTGCCTGCTCGTTACGCGTTGGATGTGAATGTGGAGAGGGCAGAAGATGTTCTAGCACATAAGAGATTGCTGCAATTTGCAGAAGACCCTGCTAATCAACCTGCCTTTGAAGTTCGCTCTGTGCAGGTTTATCCTGTCTTCAATGGAAATTCTGTTGACTCGGTCCATTCAGATTCTTTGATGAAAGAAGATGCACAAAGTTCTTACAGGTACTCTAACGGACGAGGGATTCATGCACCTCCCACCTTTGGTTCATCTCCTACTCTTGACGCACTGGCACTTCAAGCCAATAGATACCATGTTGAAGATGGAGGTAGTGCTATGAGTGCAACACCAGTCTTTTCGGGGCCTATGCATGAGATCACGTTTTCAACAGTTGACAAGCCCAAACTTCTTAGTCAGTTAACTTCATTACTTGCTGAGATTGGACTGAATATTCAAGAAGCTCATGCTTTCTCCACTGTTGATGGATTTTCTCTTGATGTTTTCGTGGTTGATGGGTGGAAATACGAGGAAACCGAGGAGCTCAAAAGTGTTCTAGAAAAGGAAATTTCAAAATTTAAGGAGCAACCTTGGTCAAAACCACATCCAATCGCTGCTGTTGGTGAGGACAATCAAACAAGGGATGAGTCCTTCCCCAGTTGCATAGAAATACCAACTGATGGAACAGATGTCTGGGAGATTGATGCAAGGCAGCTGAAATTTGAGAACAAAGTTGGATCTGGGTCATTCGGTGATCTGTACAGAGGCACTTATTGTACTCAGGAAGTTGCCATAAAAGTTCTCAAACCTGAGCGTGTCAATGCAGAGATGCTCAGGGATTTTTCACAGGAAGTTTATATATTGAGGAAGATTCGGCACAAGAATGTTGTGCAATTTATAGGAGCATGTACACGACCTCCAAACCTATGCATTGTGACAGAATTTATGTCCAAAGGAAGTGTATACGATTTTCTGCATAAGCACAAGGGTGTGTTTAAGCTTCCATCTCTACTCAAAGTAGCAATTGATGTCTCCAAGGGAATGAACTATTTGCACCAAAATAATATAATCCACAGGGATCTTAAGACTGCCAATCTTCTTATGGATGAACATGAAGTCGTTAAGGTTGCTGATTTTGGGGTTGCCAGAGTGCAGACTCAATCTGGAGTGATGACAGCTGAAACTGGTACATACCGCTGGATGGCTCCTGAGGTCATCGAACACAAACCATATGATCACAAGGCAGACGTTTTCAGTTTTGGAATAGTACTGTGGGAGCTTCTAACTGGACAAATCCCCTACTCATCCTTGACCCCGTTACAAGCGGCAGTAGGTGTGGTGCAAAAGAGCCTACGACCTACAATTCCCAAGACCACTCACCCAAGATTCGCCGAACTGCTTGAGAGATGCTGGCAACAGGACCCGACTCAAAGACCCCCCTTTTCCGATATTATAGAAATCCTGCAGAATTTGGCCAAAGAGGTTGGTAATGAGGGAGAAGATCGACGCAGACCTGGTGGTTTTTTCTCGGCACTTAGATGGGGCAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

574

Amino Acids

64.71

Weight (kDa)

5.71

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 295 - 544 2.1e-74 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 297 - 541 4e-61 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17700 AT4G35780
fragaria_vesca FvH4_1g10940 FvH4_1g10940 FvH4_1g10940
malus_domestica MD02G1122500.v1.1 MD15G1236700.v1.1
prunus_persica Prupe.7G176000_v2.0.a1
pyrus_communis pycom02g09550 pycom15g21040
rosa_chinensis RchiOBHm_Chr2g0098201
rosa_laevigata RLG00000016776
rosa_multiflora Rmu_sc0009178.1_g000003
rosa_roxburghii Rroxscaffold_2G00144160
rosa_rugosa Rorug02G0071100 Rorug02G0071200.1
rosa_samantha Rh2AG118300 Rh2BG121300 Rh2DG123000
rosa_wichuraiana Rw2G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 295, 301
AccB7I CCANNNNNTGG 1 cut(s) 848
AccBSI CCGCTC 1 cut(s) 41
AccI GTMKAC 2 cut(s) 93, 1131
AccII CGCG 1 cut(s) 203
AciI CCGC 4 cut(s) 30, 41, 1363, 1493
AclWI GGATC 2 cut(s) 913, 1254
AcoI YGGCCR 1 cut(s) 1641
AcsI RAATTY 7 cut(s) 114, 334, 735, 743, 887, 1109, 1636
AfaI GTAC 6 cut(s) 398, 929, 946, 1078, 1357, 1437
AfiI CCNNNNNNNGG 3 cut(s) 434, 848, 1369
AflII CTTAAG 1 cut(s) 1250
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 9 cut(s) 119, 181, 296, 328, 470, 491, 558, 626, 741
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 2 cut(s) 148, 1681
AleI CACNNNNGTG 1 cut(s) 1155
AluBI AGCT 9 cut(s) 27, 85, 166, 632, 712, 883, 1168, 1346, 1447
AluI AGCT 9 cut(s) 27, 85, 166, 632, 712, 883, 1168, 1346, 1447
Alw21I GWGCWC 2 cut(s) 108, 714
Alw26I GTCTC 1 cut(s) 1204
AlwI GGATC 2 cut(s) 913, 1254
AlwNI CAGNNNCTG 1 cut(s) 85
AoxI GGCC 2 cut(s) 533, 1641
ApeKI GCWGC 6 cut(s) 27, 33, 61, 256, 782, 880
ApoI RAATTY 7 cut(s) 114, 334, 735, 743, 887, 1109, 1636
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 3 cut(s) 349, 533, 1588
AsuHPI GGTGA 3 cut(s) 803, 932, 1538
AvaII GGWCC 2 cut(s) 349, 1588
AxyI CCTNAGG 1 cut(s) 1377
BalI TGGCCA 1 cut(s) 1643
BanII GRGCYC 1 cut(s) 714
BbsI GAAGAC 2 cut(s) 276, 316
Bbv12I GWGCWC 2 cut(s) 108, 714
BbvI GCAGC 6 cut(s) 14, 45, 73, 243, 769, 892
BccI CCATC 7 cut(s) 488, 647, 677, 842, 1180, 1363, 1701
BciT130I CCWGG 2 cut(s) 150, 1683
BclI TGATCA 2 cut(s) 160, 1402
BcoDI GTCTC 1 cut(s) 1204
BfaI CTAG 2 cut(s) 239, 725
BfmI CTRYAG 1 cut(s) 1631
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 2 cut(s) 295, 301
BisI GCNGC 8 cut(s) 28, 31, 34, 62, 257, 783, 881, 1494
BlsI GCNGC 8 cut(s) 29, 32, 35, 63, 258, 784, 882, 1495
BmcAI AGTACT 1 cut(s) 1437
Bme1390I CCNGG 2 cut(s) 150, 1683
Bme18I GGWCC 2 cut(s) 349, 1588
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 3 cut(s) 349, 533, 1588
BmiI GGNNCC 3 cut(s) 534, 1374, 1590
BmrFI CCNGG 2 cut(s) 150, 1683
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 4 cut(s) 367, 862, 987, 1565
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 2 cut(s) 276, 316
BpmI CTGGAG 2 cut(s) 107, 1353
Bpu10I CCTNAGC 2 cut(s) 978, 1001
BpuEI CTTGAG 1 cut(s) 1589
BsaJI CCNNGG 3 cut(s) 705, 758, 1203
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 848, 1369
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 5 cut(s) 465, 521, 825, 1357, 1459
Bse21I CCTNAGG 1 cut(s) 1377
BseBI CCWGG 2 cut(s) 150, 1683
BseDI CCNNGG 3 cut(s) 705, 758, 1203
BseGI GGATG 6 cut(s) 214, 772, 817, 1279, 1374, 1472
BseLI CCNNNNNNNGG 3 cut(s) 434, 848, 1369
BseMII CTCAG 5 cut(s) 597, 962, 969, 1015, 1368
BseNI ACTGG 5 cut(s) 465, 521, 825, 1357, 1459
BseRI GAGGAG 2 cut(s) 149, 722
BseXI GCAGC 6 cut(s) 14, 45, 73, 243, 769, 892
BsgI GTGCAG 2 cut(s) 329, 1340
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 2 cut(s) 535, 1643
BsiHKAI GWGCWC 2 cut(s) 108, 714
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 3 cut(s) 434, 848, 1369
BsmAI GTCTC 1 cut(s) 1204
BsnI GGCC 2 cut(s) 535, 1643
Bsp1286I GDGCHC 2 cut(s) 108, 714
Bsp1407I TGTACA 2 cut(s) 927, 1076
Bsp143I GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
BspACI CCGC 4 cut(s) 30, 41, 1363, 1493
BspANI GGCC 2 cut(s) 535, 1643
BspCNI CTCAG 5 cut(s) 598, 961, 970, 1014, 1369
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 3 cut(s) 534, 1374, 1590
BspMAI CTGCAG 1 cut(s) 1635
BspMI ACCTGC 2 cut(s) 295, 301
BspPI GGATC 2 cut(s) 913, 1254
BspTI CTTAAG 1 cut(s) 1250
BsrBI CCGCTC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 75
BsrGI TGTACA 2 cut(s) 927, 1076
BsrI ACTGG 5 cut(s) 465, 521, 825, 1357, 1459
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 3 cut(s) 705, 758, 1203
BssMI GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
BssNAI GTATAC 1 cut(s) 1132
BssT1I CCWWGG 2 cut(s) 758, 1203
Bst1107I GTATAC 1 cut(s) 1132
Bst2UI CCWGG 2 cut(s) 150, 1683
Bst4CI ACNGT 3 cut(s) 562, 649, 1440
BstAFI CTTAAG 1 cut(s) 1250
BstAUI TGTACA 2 cut(s) 927, 1076
BstC8I GCNNGC 3 cut(s) 57, 192, 1580
BstDEI CTNAG 7 cut(s) 581, 606, 948, 978, 1001, 1377, 1703
BstF5I GGATG 6 cut(s) 214, 772, 817, 1279, 1374, 1472
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 7 cut(s) 163, 549, 908, 925, 1249, 1405, 1672
BstMAI GTCTC 1 cut(s) 1204
BstMBI GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
BstMWI GCNNNNNNNGC 6 cut(s) 33, 52, 61, 200, 470, 512
BstNI CCWGG 2 cut(s) 150, 1683
BstNSI RCATGY 1 cut(s) 1077
BstSCI CCNGG 2 cut(s) 148, 1681
BstSFI CTRYAG 1 cut(s) 1631
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 6 cut(s) 14, 45, 73, 243, 769, 892
BstV2I GAAGAC 2 cut(s) 276, 316
BstX2I RGATCY 2 cut(s) 905, 1246
BstYI RGATCY 2 cut(s) 905, 1246
BstZ17I GTATAC 1 cut(s) 1132
Bsu36I CCTNAGG 1 cut(s) 1377
BsuRI GGCC 2 cut(s) 535, 1643
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 6 cut(s) 214, 772, 817, 1279, 1374, 1472
BtsIMutI CAGTG 3 cut(s) 458, 645, 1717
BveI ACCTGC 2 cut(s) 295, 301
Cac8I GCNNGC 3 cut(s) 57, 192, 1580
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 349, 533, 1588
CseI GACGC 2 cut(s) 464, 1683
CsiI ACCWGGT 1 cut(s) 1681
Csp6I GTAC 6 cut(s) 397, 928, 945, 1077, 1356, 1436
CviAII CATG 6 cut(s) 419, 485, 542, 635, 1074, 1280
CviQI GTAC 6 cut(s) 397, 928, 945, 1077, 1356, 1436
DdeI CTNAG 7 cut(s) 581, 606, 948, 978, 1001, 1377, 1703
DpnI GATC 7 cut(s) 162, 548, 907, 924, 1248, 1404, 1671
DpnII GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
EaeI YGGCCR 1 cut(s) 1641
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 2 cut(s) 758, 1203
Eco24I GRGCYC 1 cut(s) 714
Eco47I GGWCC 2 cut(s) 349, 1588
Eco53kI GAGCTC 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 1377
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 2 cut(s) 533, 1588
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 2 cut(s) 148, 1681
EcoT14I CCWWGG 2 cut(s) 758, 1203
EcoT22I ATGCAT 2 cut(s) 543, 1100
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 2 cut(s) 758, 1203
FaeI CATG 6 cut(s) 422, 488, 545, 638, 1077, 1283
FatI CATG 6 cut(s) 418, 484, 541, 634, 1073, 1279
FauNDI CATATG 1 cut(s) 1399
FbaI TGATCA 2 cut(s) 160, 1402
FblI GTMKAC 2 cut(s) 93, 1131
Fnu4HI GCNGC 8 cut(s) 28, 31, 34, 62, 257, 783, 881, 1494
FokI GGATG 6 cut(s) 221, 759, 824, 1286, 1381, 1459
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 8 cut(s) 28, 31, 34, 62, 257, 783, 881, 1494
FspBI CTAG 2 cut(s) 239, 725
GlaI GCGC 1 cut(s) 60
GluI GCNGC 8 cut(s) 28, 31, 34, 62, 257, 783, 881, 1494
GsuI CTGGAG 2 cut(s) 107, 1353
HaeIII GGCC 2 cut(s) 535, 1643
HapII CCGG 1 cut(s) 76
HgaI GACGC 2 cut(s) 464, 1683
HhaI GCGC 1 cut(s) 61
Hin1II CATG 6 cut(s) 422, 488, 545, 638, 1077, 1283
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 3 cut(s) 343, 565, 591
HindII GTYRAC 3 cut(s) 343, 565, 591
HindIII AAGCTT 1 cut(s) 1166
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 1 cut(s) 76
HphI GGTGA 3 cut(s) 803, 932, 1538
Hpy166II GTNNAC 6 cut(s) 94, 343, 565, 591, 1079, 1132
Hpy188I TCNGA 3 cut(s) 110, 358, 1615
Hpy188III TCNNGA 8 cut(s) 119, 452, 626, 665, 725, 950, 1332, 1376
Hpy8I GTNNAC 6 cut(s) 94, 343, 565, 591, 1079, 1132
Hpy99I CGWCG 4 cut(s) 14, 55, 114, 1677
HpyAV CCTTC 1 cut(s) 829
HpyCH4III ACNGT 3 cut(s) 562, 649, 1440
HpyCH4IV ACGT 3 cut(s) 18, 551, 1416
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 52, 61, 200, 470, 512
HpyF3I CTNAG 7 cut(s) 581, 606, 948, 978, 1001, 1377, 1703
HpySE526I ACGT 3 cut(s) 18, 551, 1416
Hsp92II CATG 6 cut(s) 422, 488, 545, 638, 1077, 1283
HspAI GCGC 1 cut(s) 59
Ksp22I TGATCA 2 cut(s) 160, 1402
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
LmnI GCTCC 6 cut(s) 111, 709, 751, 1070, 1378, 1444
Lsp1109I GCAGC 6 cut(s) 14, 45, 73, 243, 769, 892
LweI GCATC 4 cut(s) 367, 862, 987, 1565
MabI ACCWGGT 1 cut(s) 1681
MaeI CTAG 2 cut(s) 239, 725
MaeII ACGT 3 cut(s) 18, 551, 1416
MaeIII GTNAC 3 cut(s) 197, 1102, 1485
MalI GATC 7 cut(s) 162, 548, 907, 924, 1248, 1404, 1671
MbiI CCGCTC 1 cut(s) 41
MboI GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
MboII GAAGA 9 cut(s) 137, 242, 281, 316, 386, 503, 1048, 1256, 1679
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 2 cut(s) 905, 1246
MhlI GDGCHC 2 cut(s) 108, 714
MlsI TGGCCA 1 cut(s) 1643
MluI ACGCGT 1 cut(s) 201
MluNI TGGCCA 1 cut(s) 1643
MlyI GAGTC 5 cut(s) 131, 338, 824, 1318, 1588
MmeI TCCRAC 3 cut(s) 133, 186, 883
Mox20I TGGCCA 1 cut(s) 1643
Mph1103I ATGCAT 2 cut(s) 543, 1100
MroXI GAANNNNTTC 1 cut(s) 234
MscI TGGCCA 1 cut(s) 1643
MseI TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
MslI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
Msp20I TGGCCA 1 cut(s) 1643
MspA1I CMGCKG 3 cut(s) 883, 1346, 1365
MspCI CTTAAG 1 cut(s) 1250
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 150, 1683
MunI CAATTG 1 cut(s) 1191
MvaI CCWGG 2 cut(s) 150, 1683
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 6 cut(s) 33, 52, 61, 200, 470, 512
NdeI CATATG 1 cut(s) 1399
NdeII GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
NlaIII CATG 6 cut(s) 422, 488, 545, 638, 1077, 1283
NlaIV GGNNCC 3 cut(s) 534, 1374, 1590
NmeAIII GCCGAG 2 cut(s) 62, 1676
NmuCI GTSAC 1 cut(s) 1102
NsiI ATGCAT 2 cut(s) 543, 1100
NspI RCATGY 1 cut(s) 1077
OliI CACNNNNGTG 1 cut(s) 1155
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 702
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 5 cut(s) 142, 359, 415, 1039, 1554
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 848
PfoI TCCNGGA 1 cut(s) 148
PkrI GCNGC 8 cut(s) 29, 32, 35, 63, 258, 784, 882, 1495
PleI GAGTC 5 cut(s) 130, 338, 823, 1318, 1588
PpsI GAGTC 5 cut(s) 130, 338, 823, 1318, 1588
PpuMI RGGWCCY 1 cut(s) 1588
Psp124BI GAGCTC 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1588
Psp6I CCWGG 2 cut(s) 148, 1681
PspGI CCWGG 2 cut(s) 148, 1681
PspN4I GGNNCC 3 cut(s) 534, 1374, 1590
PspPI GGNCC 3 cut(s) 349, 533, 1588
PspPPI RGGWCCY 1 cut(s) 1588
PstI CTGCAG 1 cut(s) 1635
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 905, 1246
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 2 cut(s) 883, 1346
RsaI GTAC 6 cut(s) 398, 929, 946, 1078, 1357, 1437
RsaNI GTAC 6 cut(s) 397, 928, 945, 1077, 1356, 1436
RseI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
SacI GAGCTC 1 cut(s) 714
SaqAI TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
SatI GCNGC 8 cut(s) 28, 31, 34, 62, 257, 783, 881, 1494
Sau3AI GATC 7 cut(s) 160, 546, 905, 922, 1246, 1402, 1669
Sau96I GGNCC 3 cut(s) 349, 533, 1588
ScaI AGTACT 1 cut(s) 1437
SchI GAGTC 5 cut(s) 131, 338, 824, 1318, 1588
ScrFI CCNGG 2 cut(s) 150, 1683
SduI GDGCHC 2 cut(s) 108, 714
SexAI ACCWGGT 1 cut(s) 1681
SfaNI GCATC 4 cut(s) 367, 862, 987, 1565
SfcI CTRYAG 1 cut(s) 1631
SinI GGWCC 2 cut(s) 349, 1588
SmiMI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
SmlI CTYRAG 2 cut(s) 1250, 1568
SmoI CTYRAG 2 cut(s) 1250, 1568
SsiI CCGC 4 cut(s) 30, 41, 1363, 1493
SspI AATATT 1 cut(s) 622
SspMI CTAG 2 cut(s) 239, 725
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 148, 1681
StyI CCWWGG 2 cut(s) 758, 1203
TaaI ACNGT 3 cut(s) 562, 649, 1440
TaiI ACGT 3 cut(s) 21, 554, 1419
TaqI TCGA 3 cut(s) 9, 1386, 1672
TaqII GACCGA 1 cut(s) 337
TatI WGTACW 4 cut(s) 927, 944, 1076, 1435
TauI GCSGC 2 cut(s) 33, 1496
TfiI GAWTC 5 cut(s) 142, 359, 415, 1039, 1554
Tru1I TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
Tru9I TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
TscAI CASTG 2 cut(s) 465, 652
TseFI GTSAC 1 cut(s) 1102
TseI GCWGC 6 cut(s) 27, 33, 61, 256, 782, 880
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 8 cut(s) 164, 383, 407, 429, 585, 1226, 1290, 1296
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 2 cut(s) 465, 652
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 848
Vha464I CTTAAG 1 cut(s) 1250
VpaK11BI GGWCC 2 cut(s) 349, 1588
XapI RAATTY 7 cut(s) 114, 334, 735, 743, 887, 1109, 1636
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 1077
XcmI CCANNNNNNNNNTGG 2 cut(s) 491, 1267
XmiI GTMKAC 2 cut(s) 93, 1131
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 2 cut(s) 239, 725
ZrmI AGTACT 1 cut(s) 1437
Zsp2I ATGCAT 2 cut(s) 543, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.