Rh2BG121300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010681
Physical Location & Seq
Reverse (-)
10318850 .. 10325753
6904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG121300.1

Sequence Viewer

Length: 1536 bp
ATGGCGATCGACGAGGACTTGGAGAGCTGCGGCAGCAGAGCCGCTGCCGCGTTCTCGTCTCAGGCGCAGCCTCGCCACCAGCGGCAGAAGCTCGAGGTCTACAACGAGGTGCTGAGGAGAATTCAGGAGTACAATTATGAGGAGGCCAGTCTTCCTGGATTTGATGATCAGCTTTGGCTCCATTTCAATCGATTACCTGCTCGATACGCGTTGGATGTGAATGTGGAGAGGGCAGAGGATGTGCTTACACATAAGAGATTGCTGCAACTTGCAGAGGACCCTGCTTACCAACCCGCTTTTGAAGTTCGCACTGTGCAGGTTTATCCTGTGTACAATGGGATTCCTGGTGATTCGGTCCATTCAGATTCTTCAATGAAAGAAGATGCACAAAGTTCTTTTAATTATAACAGACAAGGGATTCATCCACCACCTACCTTTGGTTCGTCTCCTAATCTCGAGGCACTTTCACTTCTAGCCAATAGATCCCATGTTGATGATGGAGACAGTGCAATGAGCGTAACACCCTATGTTTCTAGGCCTATGCATGAGATCACTTTTTCAACAGTTGACAAGCCTAAACTTCTTAGTCAGTTAACTTCATTACTTGCTGATATTGGACTGAATATTCAAGAAGCTCATGCCTTTTCCACTGTTGATGGATTTTCTCTGGATGTTTTCGTAGTTGATGGTTGGCACTTAGAGGAAACTGAAGAACTTAAAAGGGTGTTGGAGAAGGAAATTTCAAAATTTAAGAAGTCAAAGCAGTATCCAGTTGCTGCAATTAGCGAGGATAACCAAATAAAAATTGAGTCTTCCCACGACGCCCTACAAATACCAACTGATGGTATAGATGTATGGGAGATTGACACAAGGCAGCTGAAATTTGAGAACAAAGTTGGATCTGGATCATTCGGTGATCTGTACAGAGGCACATATTGTAGTCAGGAAGTTGCCATCAAAGTTTTAAAACCTGAGCGTGTCAATGCAGAGATGCTGAGAGATTTTTCACAGGAAGTTTATATACTGAGGAAAATTCGGCACAAGAATGTTGTGCAATTTATTGGTGCATGTACACAACCTCCAAACATATGTATTGTGACTGAGTTTATGTCCAGGGGAAGTGTATATGACTTTCTGCATAAACAAAAGGGTGTGTTCAAGCTTCCGTCTCTGCTCAAAGTAGCAATTGATGTTTCGAAGGGAATGAATTATTTGCACCAAAATAATATAGTCCACAGAGACCTTAAGACTGCCAATCTTCTGATGGATGAACACGAAGTTGTTAAGGTTGCTGATTTTGGGGTTGCCAGAGTCCAAGCACAATCTGGAGTGATGACAGCTGAAACCGGAACATACCGATGGATGGCTCCTGAGGTCATTGAACACAAACCGTATGATCACAAGGCAGATGTTTTCAGTTTTGGTATAGTGTTGTGGGAGCTTCTAACAGGAGAACTCCCCTACTCATCCTTGACCCCATTACAAGCAGCAGTGGGTGTGGTGCAAAAGGTACTCATCATATACGATTTGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

511

Amino Acids

57.66

Weight (kDa)

5.43

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 293 - 496 1.1e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 295 - 491 1.4e-56 Protein kinase domain
Pkinase_fungal PF17667 387 - 474 6.7e-06 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17700 AT4G35780
fragaria_vesca FvH4_1g10940 FvH4_1g10940 FvH4_1g10940
malus_domestica MD02G1122500.v1.1 MD15G1236700.v1.1
prunus_persica Prupe.7G176000_v2.0.a1
pyrus_communis pycom02g09550 pycom15g21040
rosa_chinensis RchiOBHm_Chr2g0098201
rosa_laevigata RLG00000016776
rosa_multiflora Rmu_sc0009178.1_g000003
rosa_roxburghii Rroxscaffold_2G00144160
rosa_rugosa Rorug02G0071100 Rorug02G0071200.1
rosa_samantha Rh2AG118300 Rh2BG121300 Rh2DG123000
rosa_wichuraiana Rw2G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc36I ACCTGC 2 cut(s) 205, 307
AccB7I CCANNNNNTGG 1 cut(s) 842
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 50, 209
AciI CCGC 5 cut(s) 30, 42, 48, 82, 294
AclWI GGATC 3 cut(s) 477, 907, 913
AcsI RAATTY 5 cut(s) 120, 738, 746, 881, 1032
AcuI CTGAAG 1 cut(s) 729
AcyI GRCGYC 1 cut(s) 822
AfaI GTAC 5 cut(s) 131, 332, 923, 1072, 1512
AfiI CCNNNNNNNGG 3 cut(s) 437, 842, 1363
AflII CTTAAG 1 cut(s) 1244
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 8 cut(s) 187, 302, 372, 561, 629, 744, 1159, 1382
AjnI CCWGG 3 cut(s) 154, 343, 1112
AluBI AGCT 8 cut(s) 27, 91, 172, 635, 877, 1162, 1340, 1441
AluI AGCT 8 cut(s) 27, 91, 172, 635, 877, 1162, 1340, 1441
Alw26I GTCTC 5 cut(s) 63, 450, 495, 1173, 1233
AlwI GGATC 3 cut(s) 477, 907, 913
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 2 cut(s) 92, 455
AoxI GGCC 2 cut(s) 144, 536
ApeKI GCWGC 8 cut(s) 27, 33, 44, 67, 262, 776, 874, 1487
ApoI RAATTY 5 cut(s) 120, 738, 746, 881, 1032
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 277, 355
AsuHPI GGTGA 2 cut(s) 359, 926
AsuII TTCGAA 1 cut(s) 1196
AvaI CYCGRG 2 cut(s) 92, 455
AvaII GGWCC 2 cut(s) 277, 355
AxyI CCTNAGG 1 cut(s) 1371
BarI GAAGNNNNNNTAC 2 cut(s) 1005, 1037
BbsI GAAGAC 2 cut(s) 143, 804
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 8 cut(s) 14, 31, 45, 79, 249, 763, 886, 1499
BccI CCATC 8 cut(s) 491, 650, 680, 836, 962, 1258, 1353, 1357
BciT130I CCWGG 3 cut(s) 156, 345, 1114
BciVI GTATCC 1 cut(s) 777
BclI TGATCA 2 cut(s) 166, 1396
BcoDI GTCTC 5 cut(s) 63, 450, 495, 1173, 1233
BfaI CTAG 2 cut(s) 473, 534
BfrI CTTAAG 1 cut(s) 1244
BfuAI ACCTGC 2 cut(s) 205, 307
BfuI GTATCC 1 cut(s) 777
Bme1390I CCNGG 3 cut(s) 156, 345, 1114
Bme18I GGWCC 2 cut(s) 277, 355
BmeT110I CYCGRG 2 cut(s) 92, 455
BmgT120I GGNCC 2 cut(s) 277, 355
BmiI GGNNCC 3 cut(s) 179, 279, 1368
BmrFI CCNGG 3 cut(s) 156, 345, 1114
BmsI GCATC 2 cut(s) 373, 981
BpiI GAAGAC 2 cut(s) 143, 804
BpmI CTGGAG 1 cut(s) 1347
Bpu10I CCTNAGC 2 cut(s) 113, 972
Bpu14I TTCGAA 1 cut(s) 1196
Bsa29I ATCGAT 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 904
BsaHI GRCGYC 1 cut(s) 822
BsaI GGTCTC 1 cut(s) 1233
BsaJI CCNNGG 1 cut(s) 1113
BsaWI WCCGGW 1 cut(s) 1346
BsaXI ACNNNNNCTCC 2 cut(s) 1063, 1093
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 842, 1363
Bse1I ACTGG 2 cut(s) 147, 770
Bse21I CCTNAGG 1 cut(s) 1371
Bse3DI GCAATG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 904
BseBI CCWGG 3 cut(s) 156, 345, 1114
BseCI ATCGAT 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 1113
BseGI GGATG 7 cut(s) 220, 244, 421, 676, 1273, 1368, 1466
BseJI GATNNNNATC 1 cut(s) 904
BseLI CCNNNNNNNGG 3 cut(s) 437, 842, 1363
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 7 cut(s) 74, 104, 963, 986, 1016, 1092, 1362
BseNI ACTGG 2 cut(s) 147, 770
BseRI GAGGAG 2 cut(s) 130, 155
BseXI GCAGC 8 cut(s) 14, 31, 45, 79, 249, 763, 886, 1499
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 2 cut(s) 50, 209
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 146, 538
BshVI ATCGAT 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 2 cut(s) 92, 455
BsiSI CCGG 1 cut(s) 1347
BslI CCNNNNNNNGG 3 cut(s) 437, 842, 1363
BsmAI GTCTC 5 cut(s) 63, 450, 495, 1173, 1233
BsmBI CGTCTC 3 cut(s) 63, 450, 1173
BsnI GGCC 2 cut(s) 146, 538
Bso31I GGTCTC 1 cut(s) 1233
BsoBI CYCGRG 2 cut(s) 92, 455
Bsp119I TTCGAA 1 cut(s) 1196
Bsp1407I TGTACA 3 cut(s) 330, 921, 1070
Bsp143I GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
BspACI CCGC 5 cut(s) 30, 42, 48, 82, 294
BspANI GGCC 2 cut(s) 146, 538
BspCNI CTCAG 7 cut(s) 73, 105, 964, 987, 1017, 1093, 1363
BspDI ATCGAT 1 cut(s) 190
BspFNI CGCG 2 cut(s) 50, 209
BspLI GGNNCC 3 cut(s) 179, 279, 1368
BspMI ACCTGC 2 cut(s) 205, 307
BspPI GGATC 3 cut(s) 477, 907, 913
BspT104I TTCGAA 1 cut(s) 1196
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 1233
BsrDI GCAATG 1 cut(s) 516
BsrGI TGTACA 3 cut(s) 330, 921, 1070
BsrI ACTGG 2 cut(s) 147, 770
BssECI CCNNGG 1 cut(s) 1113
BssMI GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
BssNI GRCGYC 1 cut(s) 822
Bst2UI CCWGG 3 cut(s) 156, 345, 1114
Bst4CI ACNGT 5 cut(s) 313, 506, 565, 652, 1392
BstACI GRCGYC 1 cut(s) 822
BstAFI CTTAAG 1 cut(s) 1244
BstAUI TGTACA 3 cut(s) 330, 921, 1070
BstBI TTCGAA 1 cut(s) 1196
BstDEI CTNAG 9 cut(s) 60, 113, 584, 697, 972, 995, 1025, 1101, 1371
BstF5I GGATG 7 cut(s) 220, 244, 421, 676, 1273, 1368, 1466
BstFNI CGCG 2 cut(s) 50, 209
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 8 cut(s) 9, 169, 485, 552, 902, 908, 919, 1399
BstMAI GTCTC 5 cut(s) 63, 450, 495, 1173, 1233
BstMBI GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 33, 47, 88, 206
BstNI CCWGG 3 cut(s) 156, 345, 1114
BstNSI RCATGY 1 cut(s) 1071
BstSCI CCNGG 3 cut(s) 154, 343, 1112
BstUI CGCG 2 cut(s) 50, 209
BstV1I GCAGC 8 cut(s) 14, 31, 45, 79, 249, 763, 886, 1499
BstV2I GAAGAC 2 cut(s) 143, 804
BstX2I RGATCY 2 cut(s) 482, 899
BstYI RGATCY 2 cut(s) 482, 899
Bsu15I ATCGAT 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 1371
BsuI GTATCC 1 cut(s) 777
BsuRI GGCC 2 cut(s) 146, 538
BsuTUI ATCGAT 1 cut(s) 190
BtsCI GGATG 7 cut(s) 220, 244, 421, 676, 1273, 1368, 1466
BtsI GCAGTG 1 cut(s) 1497
BtsIMutI CAGTG 4 cut(s) 309, 511, 648, 1497
BveI ACCTGC 2 cut(s) 205, 307
CaiI CAGNNNCTG 1 cut(s) 776
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 277, 355
ClaI ATCGAT 1 cut(s) 190
CseI GACGC 1 cut(s) 830
Csp6I GTAC 5 cut(s) 130, 331, 922, 1071, 1511
CviAII CATG 4 cut(s) 488, 545, 638, 1068
CviQI GTAC 5 cut(s) 130, 331, 922, 1071, 1511
DdeI CTNAG 9 cut(s) 60, 113, 584, 697, 972, 995, 1025, 1101, 1371
DpnI GATC 8 cut(s) 8, 168, 484, 551, 901, 907, 918, 1398
DpnII GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
DraI TTTAAA 1 cut(s) 966
Eco147I AGGCCT 1 cut(s) 538
Eco31I GGTCTC 1 cut(s) 1233
Eco47I GGWCC 2 cut(s) 277, 355
Eco57I CTGAAG 1 cut(s) 729
Eco81I CCTNAGG 1 cut(s) 1371
Eco88I CYCGRG 2 cut(s) 92, 455
EcoO109I RGGNCCY 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 3 cut(s) 154, 343, 1112
EcoT22I ATGCAT 1 cut(s) 546
Esp3I CGTCTC 3 cut(s) 63, 450, 1173
FaeI CATG 4 cut(s) 491, 548, 641, 1071
FatI CATG 4 cut(s) 487, 544, 637, 1067
FauI CCCGC 1 cut(s) 301
FauNDI CATATG 1 cut(s) 1088
FbaI TGATCA 2 cut(s) 166, 1396
FblI GTMKAC 1 cut(s) 99
FokI GGATG 7 cut(s) 227, 251, 408, 683, 1280, 1375, 1453
FspBI CTAG 2 cut(s) 473, 534
GlaI GCGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 1347
HaeIII GGCC 2 cut(s) 146, 538
HapII CCGG 1 cut(s) 1347
HgaI GACGC 1 cut(s) 830
HhaI GCGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 822
Hin1II CATG 4 cut(s) 491, 548, 641, 1071
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 2 cut(s) 568, 594
HindII GTYRAC 2 cut(s) 568, 594
HindIII AAGCTT 1 cut(s) 1160
HinfI GANTC 6 cut(s) 340, 350, 365, 418, 809, 1311
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 1 cut(s) 1347
HphI GGTGA 2 cut(s) 359, 926
Hpy166II GTNNAC 6 cut(s) 100, 331, 568, 594, 1073, 1234
Hpy188I TCNGA 2 cut(s) 364, 1263
Hpy188III TCNNGA 8 cut(s) 125, 455, 629, 668, 903, 944, 1326, 1370
Hpy8I GTNNAC 6 cut(s) 100, 331, 568, 594, 1073, 1234
Hpy99I CGWCG 2 cut(s) 14, 824
HpyAV CCTTC 2 cut(s) 727, 1192
HpyCH4III ACNGT 5 cut(s) 313, 506, 565, 652, 1392
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 47, 88, 206
HpyF3I CTNAG 9 cut(s) 60, 113, 584, 697, 972, 995, 1025, 1101, 1371
Hsp92I GRCGYC 1 cut(s) 822
Hsp92II CATG 4 cut(s) 491, 548, 641, 1071
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 2 cut(s) 166, 1396
KspAI GTTAAC 1 cut(s) 594
Kzo9I GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
LmnI GCTCC 3 cut(s) 183, 1372, 1438
Lsp1109I GCAGC 8 cut(s) 14, 31, 45, 79, 249, 763, 886, 1499
LweI GCATC 2 cut(s) 373, 981
MaeI CTAG 2 cut(s) 473, 534
MaeIII GTNAC 2 cut(s) 517, 1096
MalI GATC 8 cut(s) 8, 168, 484, 551, 901, 907, 918, 1398
MboI GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
MboII GAAGA 6 cut(s) 143, 360, 392, 722, 804, 1250
MfeI CAATTG 1 cut(s) 1185
MflI RGATCY 2 cut(s) 482, 899
MluI ACGCGT 1 cut(s) 207
MlyI GAGTC 2 cut(s) 818, 1320
MmeI TCCRAC 3 cut(s) 192, 708, 877
Mph1103I ATGCAT 1 cut(s) 546
MseI TTAA 7 cut(s) 399, 593, 717, 750, 965, 1245, 1284
MslI CAYNNNNRTG 3 cut(s) 492, 1044, 1357
MspA1I CMGCKG 4 cut(s) 44, 82, 877, 1340
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 1 cut(s) 1347
MspR9I CCNGG 3 cut(s) 156, 345, 1114
MunI CAATTG 1 cut(s) 1185
MvaI CCWGG 3 cut(s) 156, 345, 1114
MvnI CGCG 2 cut(s) 50, 209
MwoI GCNNNNNNNGC 4 cut(s) 33, 47, 88, 206
NdeI CATATG 1 cut(s) 1088
NdeII GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
NlaIII CATG 4 cut(s) 491, 548, 641, 1071
NlaIV GGNNCC 3 cut(s) 179, 279, 1368
NmuCI GTSAC 1 cut(s) 1096
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 1 cut(s) 1071
NspV TTCGAA 1 cut(s) 1196
PaeR7I CTCGAG 2 cut(s) 92, 455
PceI AGGCCT 1 cut(s) 538
PfeI GAWTC 4 cut(s) 340, 350, 365, 418
PflMI CCANNNNNTGG 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 154
Ple19I CGATCG 1 cut(s) 9
PleI GAGTC 2 cut(s) 817, 1319
PpsI GAGTC 2 cut(s) 817, 1319
PpuMI RGGWCCY 1 cut(s) 277
PsiI TTATAA 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 3 cut(s) 154, 343, 1112
PspGI CCWGG 3 cut(s) 154, 343, 1112
PspN4I GGNNCC 3 cut(s) 179, 279, 1368
PspPI GGNCC 2 cut(s) 277, 355
PspPPI RGGWCCY 1 cut(s) 277
PspXI VCTCGAGB 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 2 cut(s) 482, 899
PvuI CGATCG 1 cut(s) 9
PvuII CAGCTG 2 cut(s) 877, 1340
RsaI GTAC 5 cut(s) 131, 332, 923, 1072, 1512
RsaNI GTAC 5 cut(s) 130, 331, 922, 1071, 1511
RseI CAYNNNNRTG 3 cut(s) 492, 1044, 1357
SaqAI TTAA 7 cut(s) 399, 593, 717, 750, 965, 1245, 1284
Sau3AI GATC 8 cut(s) 6, 166, 482, 549, 899, 905, 916, 1396
Sau96I GGNCC 2 cut(s) 277, 355
SchI GAGTC 2 cut(s) 818, 1320
ScrFI CCNGG 3 cut(s) 156, 345, 1114
SfaNI GCATC 2 cut(s) 373, 981
Sfr274I CTCGAG 2 cut(s) 92, 455
SfuI TTCGAA 1 cut(s) 1196
SinI GGWCC 2 cut(s) 277, 355
SlaI CTCGAG 2 cut(s) 92, 455
SmiMI CAYNNNNRTG 3 cut(s) 492, 1044, 1357
SmlI CTYRAG 3 cut(s) 92, 455, 1244
SmoI CTYRAG 3 cut(s) 92, 455, 1244
SseBI AGGCCT 1 cut(s) 538
SsiI CCGC 5 cut(s) 30, 42, 48, 82, 294
SspI AATATT 1 cut(s) 625
SspMI CTAG 2 cut(s) 473, 534
StuI AGGCCT 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 154, 343, 1112
TaaI ACNGT 5 cut(s) 313, 506, 565, 652, 1392
TaqI TCGA 6 cut(s) 9, 93, 190, 202, 456, 1196
TaqII GACCGA 1 cut(s) 343
TatI WGTACW 4 cut(s) 129, 330, 921, 1070
TauI GCSGC 4 cut(s) 33, 44, 50, 85
TfiI GAWTC 4 cut(s) 340, 350, 365, 418
Tru1I TTAA 7 cut(s) 399, 593, 717, 750, 965, 1245, 1284
Tru9I TTAA 7 cut(s) 399, 593, 717, 750, 965, 1245, 1284
TscAI CASTG 4 cut(s) 316, 511, 655, 1497
TseFI GTSAC 1 cut(s) 1096
TseI GCWGC 8 cut(s) 27, 33, 44, 67, 262, 776, 874, 1487
Tsp45I GTSAC 1 cut(s) 1096
TspDTI ATGAA 5 cut(s) 389, 410, 588, 1220, 1284
TspGWI ACGGA 1 cut(s) 1155
TspRI CASTG 4 cut(s) 316, 511, 655, 1497
Van91I CCANNNNNTGG 1 cut(s) 842
Vha464I CTTAAG 1 cut(s) 1244
VpaK11BI GGWCC 2 cut(s) 277, 355
XapI RAATTY 5 cut(s) 120, 738, 746, 881, 1032
XceI RCATGY 1 cut(s) 1071
XcmI CCANNNNNNNNNTGG 3 cut(s) 494, 1261, 1322
XhoI CTCGAG 2 cut(s) 92, 455
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 473, 534
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.