Rorug02G0071100

Thiosulfate sulfurtransferase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005733
Physical Location & Seq
Reverse (-)
5523905 .. 5531221
7317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0071100.1

Sequence Viewer

Length: 3609 bp
ATGGGAGCGTCACCGGATGCCAACGGAGATATAGGTATGAGTAGCAAGGGAAAGGGCTTTTCCAATGGGGATATTGAGGACATGGATATTGATGACATGGATTTTGAGGATGATGAAGTTGATGATTCGACTAGCATTCATGATCTGGGAGAAGTGTTTCTCAAGGATTTCTGTAAGGAGGCGTCAGTGTCATTCTTCAAGGAGTATGGCCTTCTCAGTCACCAGATAAACTCATACAATGACTTCGTTAAAAATGGCATACAAAGGGTCTTTGATTCTTTCGGTGAGATTGTAGTTGAGCCTGGGTATGACCCATCAAAGAAGGGAGATGCTGAGTGGCGATTTGCATCTGTCAAATTTGGGAAGGTCACCCTTAACCGACCCTGTTTTTGGGGAGGTTCTGACAATGATAAGGAGTATAATATGTTACCCAGACATGCTAGGCTTCAGAACATGACATACTCAGCCAGGATGAAAGTCAATATTACGGTCCAGGTATATACTCAAAATCTTGTCAAAAGTGACAAGTTTAAAACTGGAAGAGAGCAATATCTGGACAAGCAGACTGTGAGTACTGACAGCAGGGACATCATAATTGGTCGGGTTCCTGTAATGGTGAAGTCTGACTTGTGTTGGATGAAGGAAGTCGAGAAAGGTGACTGTGAGTTTGACCAGGGAGGCTACTTTATAATCAAGGGTGCAGAGAAGACGTTTATCGCTCAAGAACAGAGCTGTTTGAAGAGACTTCTGATTACAAATAATCAGGGTCTCACAGTAGCATATAGGTCAGAGGTCAAGCGGCATAGGCTCATAATTAGATTATCCGGGATTTCGAAACTTGAAAATGTTGAAGGAGTGGAGAAAGTCCTGTCAGTATACTTTATGTCAACAGAAATCCCTGTTTGGATTTGGTTTTTCGCCCTTGGGGTTAGTTCAGACAAAGAAGTTATCGATCTTATTGATTATGGCAAAGAAGATGCAAGCATTTCAAACATACTGTTTGCCTCAATTCGTGATGCTGACGAGAGAAAAGATGACTTTCGTAGGGGGAAGAATGCTCTGAGACATGTTGAAGTAGCGATAAAGAATACGACGTTTCCACCTGGGGAAAGCATTGAGGACTGCATTAGCATGTATCTGTTTCCTAATCTGAGAGGCATGAAGCAGAAGGCTCGGTTTCTAGGGTATATGGTGAAGTGTCTTTTACAGACCTTCAGTGGCCGAAGGAAATGCGATAATAGGGATGATTTTAGAAACAAGAGGTTGGAGTTAGCAGGTGAGCTTCTAGAACGAGAGCTGAAGGCACATATTTCACATGCACGGAGGCGCATGGCAAAGGCTCTGCAGAGAGATCTATGTGGTGATCGTGTTGTCCGCCCGGTGGAGCACTATCTAGATGCATCTATAGTTACCAGTGGTGTTTCCAGAGCATTCTCTACTGGAGCATGGTGTCATCCTTTCAAGAGGATGGAGAGGATATCAGGTGTGGTCGCAAATGTTGGGCGAGCAAATCCACTGCAGACAATGATTGATATGAGGAAGACACGCCAGCAAGTTCAATACACTGGCAAGGCTGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGTAAGGTTTGCTTTCTCTCAACCCCAGATGGTGAGAACTGTGGACTTGTAAAAAATTTGGCTACCACTGCTCTTGTAAGTACAAACATATTGGAGCCCATAATGCCCAAATTGTTCCGATGTGGCATGGAGGAGTTGGTAGATGATACTTCTACTTCACTGGATGGGAAGCATAAAGTTTTTCTGAATGGTGACTGGGCTGGCGTTTGTGTAGATTCCCATGGTTTTGTCATGAAACTAAGGACATTGCGACGCGGGAAAAAGTTGCCACACGAGGTGGAAATCAAAAGAGATGAACCCCAAGGAGAAGTGCGCATATTTTCTGATGCTGGAAGGATTCTACGTCCTCTCTTGGTAGTCCAGAATTTGAAGAAGATTAAAGCAGCAAAAGGAGAAAAACTTACCTTCAAAGATTTTCTTGACAAGGGAATAATTGAGCTCATTGGCCCGGAAGAAGAAGAGGATTGCCGTACAGCATGGGCCATTAAGTATCTGTTTATGGAAGAACAGGGAAACTCAGTTGTCAAGTACACACACTGTGAGCTGGACATGTCGTTTTTGTTGGGTTTAAGCTGTGGTATCATTCCCTTTGCAAATCATGATCATGCAAGGAGGGTGCTGTACCAGTCTCAGAAGCACTCTCAGCAGGCCATTGGTTTTTCTACTACAAATCCTAACCTCAGAGTGGATACTTTGTCTCACAACTTACACTACCCCCAGAGGCCGCTCTTTCGTACAATGATATCTGATTCCCTTGGAAAACCAGGATACCCGGTGGGACATAACGGTATCATGCCAAAGCCTGAGTACTTCAATGGCCAGAATGCAATTGTTGCAGTCAACGTGCACCTCGGATATAACCAAGAAGATTCAATAGTAATGAACCGGGCATCACTAGAACGTGGTATGTTCCGATCTGAGCACATAAGGAGTTACAAGGCTGAAGTTGAAAACAAGCTCTCATTGGAAAAAAGACGAAAACCAGATGAGTGTGTAAATTTTGGCAAAATCCAGAGTAAATTTGGACGTGTTGACAGCCTTGATGATGATGGATTCCCCTGTGTTGGTGCAAATCTGCAGAATGGGGATATTATTATTGGGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAATTGAAGCATACTGAAAGGGGAATGGTGCAGAAGGTTGTGCTTTCCTCTAATGACGATGGCAAGAATTTTGCTGTTGTATCGTTAAGACAGGTTCGTTCTCCTTCTCTTGGAGACAAGTTCTCAAGCATGCATGGGCAAAAGGGTGTCCTAGGTTTTTTGGAGTCTCAAGAGAATTTCCCTTTCACAATACAAGGGATAGTTCCAGATATTGTAATTAATCCACATGCGTTTCCTTCAAGACAAACTCCCGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGTTCAAAGAGATACGCGACCCCTTTCTCTACTCTCTCTGTTGATGACATCACAGACCAGCTTCATAGAGCTGGATTTTCAAGATGGGGAAATGAGAGGGTATACAATGGTCGAACTGGTGAAATGGTTCGCCAACTGATATTCATGGGCCCAACATTCTATCAACGTCTGATTCACATGTCTGAAGACAAAGTAAAGTTTAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGCGATTTGGCGGGATCAAGTTTGGTGAGATGGAGCGCGACTGTCTTATAGCCCATGGAGCATCAGCAAACTTGCATGAACGCCTCTTCACTCTCAGTGACTCCTCCCTGATGAATGTTTGCCAGAAATGCCAGAATGCAGCTAATGTGATAGAGCGGAAGGTCACTGGTGGCCGCAAAATCAGGGGTCCCTATTGCCGGGTCTGCGACTCTGCTGATGACATTGTGAGGGTGAATGTGCCATATGGGGCCAAGCTATTGTGCCAGGAGCTATTCAGCATGGGCATCAGTCTCAAGTTTGAAACCAGGCTCTGTTGA

Protein Analysis

1202

Amino Acids

135.55

Weight (kDa)

8.71

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 70 - 453 7.1e-35 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 284 - 423 7.2e-11 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 502 - 561 6.2e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 596 - 657 5e-21 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 672 - 719 3.4e-06 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 728 - 1099 7.6e-111 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1101 - 1198 2.8e-26 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AarI CACCTGC 1 cut(s) 1265
Acc16I TGCGCA 1 cut(s) 1922
Acc36I ACCTGC 1 cut(s) 1265
AccBSI CCGCTC 2 cut(s) 2335, 3448
AccI GTMKAC 2 cut(s) 876, 3141
AccII CGCG 3 cut(s) 1863, 3056, 3330
AciI CCGC 8 cut(s) 799, 1375, 1592, 1863, 2333, 3303, 3448, 3466
AclWI GGATC 1 cut(s) 3314
AcoI YGGCCR 3 cut(s) 1219, 2425, 3463
AcsI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
AcuI CTGAAG 5 cut(s) 431, 1198, 1319, 2571, 3243
AcyI GRCGYC 1 cut(s) 182
AdeI CACNNNGTG 2 cut(s) 1885, 2147
AfaI GTAC 8 cut(s) 574, 1690, 2080, 2138, 2231, 2344, 2417, 2728
AfiI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
AflIII ACRYGT 4 cut(s) 1066, 2157, 2635, 3216
AjiI CACGTC 1 cut(s) 2636
AjnI CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
Alw21I GWGCWC 4 cut(s) 1389, 2049, 2457, 2532
Alw26I GTCTC 8 cut(s) 736, 773, 1057, 2241, 2310, 2850, 2913, 3587
Alw44I GTGCAC 1 cut(s) 2453
AlwI GGATC 1 cut(s) 3314
AlwNI CAGNNNCTG 1 cut(s) 3603
ApaI GGGCCC 1 cut(s) 3191
ApaLI GTGCAC 1 cut(s) 2453
ApeKI GCWGC 4 cut(s) 1991, 3011, 3274, 3431
ApoI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
ArsI GACNNNNNNTTYG 8 cut(s) 86, 118, 372, 404, 650, 682, 2980, 3012
AseI ATTAAT 1 cut(s) 2961
Asp700I GAANNNNTTC 2 cut(s) 156, 3165
AspA2I CCTAGG 1 cut(s) 2893
AspLEI GCGC 3 cut(s) 1329, 1923, 3330
AspS9I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
AsuC2I CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
AsuII TTCGAA 1 cut(s) 833
AvaII GGWCC 2 cut(s) 490, 3479
AvrII CCTAGG 1 cut(s) 2893
BaeGI GKGCMC 2 cut(s) 2457, 3191
BalI TGGCCA 1 cut(s) 2427
BanII GRGCYC 3 cut(s) 1707, 2049, 3191
BauI CACGAG 1 cut(s) 1880
BbsI GAAGAC 3 cut(s) 713, 1547, 3231
Bbv12I GWGCWC 4 cut(s) 1389, 2049, 2457, 2532
BbvI GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BccI CCATC 8 cut(s) 322, 1462, 1631, 1766, 2651, 2795, 3117, 3316
BceAI ACGGC 1 cut(s) 2061
BcgI CGANNNNNNTGC 4 cut(s) 1154, 1188, 1212, 1246
BciT130I CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BciVI GTATCC 2 cut(s) 2290, 2369
BclI TGATCA 2 cut(s) 2209, 2731
BcnI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
BcoDI GTCTC 8 cut(s) 736, 773, 1057, 2241, 2310, 2850, 2913, 3587
BfaI CTAG 8 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894, 3005
BfmI CTRYAG 4 cut(s) 1343, 1404, 1517, 2684
BfuAI ACCTGC 1 cut(s) 1265
BfuI GTATCC 2 cut(s) 2290, 2369
BglII AGATCT 1 cut(s) 1351
BisI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
BlnI CCTAGG 1 cut(s) 2893
BlsI GCNGC 7 cut(s) 801, 1993, 2334, 3013, 3276, 3433, 3467
BmcAI AGTACT 2 cut(s) 574, 2417
Bme18I GGWCC 2 cut(s) 490, 3479
BmgBI CACGTC 1 cut(s) 2636
BmgT120I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
BmiI GGNNCC 6 cut(s) 606, 1704, 3189, 3480, 3481, 3541
BmrI ACTGGG 3 cut(s) 1615, 1813, 3258
BmuI ACTGGG 3 cut(s) 1615, 1813, 3258
BpiI GAAGAC 3 cut(s) 713, 1547, 3231
BpmI CTGGAG 2 cut(s) 1461, 1596
Bpu14I TTCGAA 1 cut(s) 833
BpuEI CTTGAG 5 cut(s) 146, 705, 2851, 2895, 3569
BpuMI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
Bsa29I ATCGAT 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 773
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 2701, 2731
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
Bse3DI GCAATG 1 cut(s) 1853
Bse8I GATNNNNATC 1 cut(s) 346
BseBI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BseCI ATCGAT 1 cut(s) 951
BseGI GGATG 9 cut(s) 22, 115, 477, 642, 1249, 1453, 1473, 1594, 1777
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
BseMI GCAATG 1 cut(s) 1853
BseRI GAGGAG 2 cut(s) 1754, 3385
BseSI GKGCMC 2 cut(s) 2457, 3191
BseXI GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BsgI GTGCAG 3 cut(s) 720, 2734, 2792
Bsh1236I CGCG 3 cut(s) 1863, 3056, 3330
BshVI ATCGAT 1 cut(s) 951
BsiHKAI GWGCWC 4 cut(s) 1389, 2049, 2457, 2532
BsiSI CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
BslFI GGGAC 3 cut(s) 599, 2400, 3465
BslI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
BsmAI GTCTC 8 cut(s) 736, 773, 1057, 2241, 2310, 2850, 2913, 3587
BsmFI GGGAC 3 cut(s) 599, 2400, 3465
BsmI GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
Bso31I GGTCTC 1 cut(s) 773
Bsp119I TTCGAA 1 cut(s) 833
Bsp120I GGGCCC 1 cut(s) 3187
Bsp1286I GDGCHC 6 cut(s) 1389, 1707, 2049, 2457, 2532, 3191
Bsp143I GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
Bsp19I CCATGG 2 cut(s) 1828, 3346
BspACI CCGC 8 cut(s) 799, 1375, 1592, 1863, 2333, 3303, 3448, 3466
BspDI ATCGAT 1 cut(s) 951
BspFNI CGCG 3 cut(s) 1863, 3056, 3330
BspHI TCATGA 3 cut(s) 139, 1839, 2206
BspLI GGNNCC 6 cut(s) 606, 1704, 3189, 3480, 3481, 3541
BspMAI CTGCAG 3 cut(s) 1347, 1521, 2688
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 1 cut(s) 3314
BspT104I TTCGAA 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 773
BsrBI CCGCTC 2 cut(s) 2335, 3448
BsrDI GCAATG 1 cut(s) 1853
BssMI GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
BssNAI GTATAC 2 cut(s) 877, 3142
BssNI GRCGYC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 1880
BssT1I CCWWGG 6 cut(s) 922, 1828, 1909, 2362, 2893, 3346
Bst1107I GTATAC 2 cut(s) 877, 3142
Bst2BI CACGAG 1 cut(s) 1880
Bst2UI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
Bst4CI ACNGT 9 cut(s) 490, 568, 662, 775, 999, 1649, 2147, 2396, 3335
Bst6I CTCTTC 4 cut(s) 535, 734, 2061, 3383
BstACI GRCGYC 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 2441
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 6 cut(s) 982, 1506, 1550, 1810, 2256, 2873
BstDSI CCRYGG 2 cut(s) 1828, 3346
BstEII GGTNACC 1 cut(s) 367
BstF5I GGATG 9 cut(s) 22, 115, 477, 642, 1249, 1453, 1473, 1594, 1777
BstFNI CGCG 3 cut(s) 1863, 3056, 3330
BstHHI GCGC 3 cut(s) 1329, 1923, 3330
BstKTI GATC 8 cut(s) 145, 955, 1354, 1366, 2212, 2525, 2734, 3309
BstMAI GTCTC 8 cut(s) 736, 773, 1057, 2241, 2310, 2850, 2913, 3587
BstMBI GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
BstMWI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 1711, 2251, 2441, 3350, 3420, 3495
BstNI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BstNSI RCATGY 8 cut(s) 440, 1070, 1135, 1319, 2161, 2875, 2972, 3220
BstPI GGTNACC 1 cut(s) 367
BstSFI CTRYAG 4 cut(s) 1343, 1404, 1517, 2684
BstSLI GKGCMC 2 cut(s) 2457, 3191
BstUI CGCG 3 cut(s) 1863, 3056, 3330
BstV1I GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BstV2I GAAGAC 3 cut(s) 713, 1547, 3231
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BstZ17I GTATAC 2 cut(s) 877, 3142
Bsu15I ATCGAT 1 cut(s) 951
BsuI GTATCC 2 cut(s) 2290, 2369
BsuTUI ATCGAT 1 cut(s) 951
BtgI CCRYGG 2 cut(s) 1828, 3346
BtrI CACGTC 1 cut(s) 2636
BtsCI GGATG 9 cut(s) 22, 115, 477, 642, 1249, 1453, 1473, 1594, 1777
BtsI GCAGTG 3 cut(s) 1514, 1674, 2722
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 6 cut(s) 982, 1506, 1550, 1810, 2256, 2873
CaiI CAGNNNCTG 1 cut(s) 3603
CciI TCATGA 3 cut(s) 139, 1839, 2206
CfoI GCGC 3 cut(s) 1329, 1923, 3330
Cfr13I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
ClaI ATCGAT 1 cut(s) 951
CseI GACGC 2 cut(s) 171, 1869
Csp6I GTAC 8 cut(s) 573, 1689, 2079, 2137, 2230, 2343, 2416, 2727
CspCI CAANNNNNGTGG 2 cut(s) 1663, 1698
CviQI GTAC 8 cut(s) 573, 1689, 2079, 2137, 2230, 2343, 2416, 2727
DpnI GATC 8 cut(s) 144, 954, 1353, 1365, 2211, 2524, 2733, 3308
DpnII GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
DraI TTTAAA 1 cut(s) 532
DraIII CACNNNGTG 2 cut(s) 1885, 2147
EaeI YGGCCR 3 cut(s) 1219, 2425, 3463
Eam1104I CTCTTC 4 cut(s) 535, 734, 2061, 3383
EarI CTCTTC 4 cut(s) 535, 734, 2061, 3383
EciI GGCGGA 1 cut(s) 1364
Ecl136II GAGCTC 1 cut(s) 2047
Eco130I CCWWGG 6 cut(s) 922, 1828, 1909, 2362, 2893, 3346
Eco24I GRGCYC 3 cut(s) 1707, 2049, 3191
Eco31I GGTCTC 1 cut(s) 773
Eco32I GATATC 3 cut(s) 1479, 1589, 2352
Eco47I GGWCC 2 cut(s) 490, 3479
Eco53kI GAGCTC 1 cut(s) 2047
Eco57I CTGAAG 5 cut(s) 431, 1198, 1319, 2571, 3243
Eco91I GGTNACC 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 2047
EcoO109I RGGNCCY 1 cut(s) 3479
EcoO65I GGTNACC 1 cut(s) 367
EcoRII CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
EcoRV GATATC 3 cut(s) 1479, 1589, 2352
EcoT14I CCWWGG 6 cut(s) 922, 1828, 1909, 2362, 2893, 3346
EcoT22I ATGCAT 2 cut(s) 1402, 2877
EcoT38I GRGCYC 3 cut(s) 1707, 2049, 3191
ErhI CCWWGG 6 cut(s) 922, 1828, 1909, 2362, 2893, 3346
FalI AAGNNNNNCTT 6 cut(s) 611, 643, 1604, 1636, 2010, 2042
FaqI GGGAC 3 cut(s) 599, 2400, 3465
FauI CCCGC 2 cut(s) 1856, 3296
FauNDI CATATG 1 cut(s) 3535
FbaI TGATCA 2 cut(s) 2209, 2731
FblI GTMKAC 2 cut(s) 876, 3141
Fnu4HI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
FokI GGATG 9 cut(s) 29, 122, 484, 649, 1256, 1440, 1480, 1581, 1784
FriOI GRGCYC 3 cut(s) 1707, 2049, 3191
Fsp4HI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
FspAI RTGCGCAY 1 cut(s) 1922
FspBI CTAG 8 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894, 3005
FspI TGCGCA 1 cut(s) 1922
GlaI GCGC 3 cut(s) 1328, 1922, 3329
GluI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
GsuI CTGGAG 2 cut(s) 1461, 1596
HapII CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
HgaI GACGC 2 cut(s) 171, 1869
HhaI GCGC 3 cut(s) 1329, 1923, 3330
Hin1I GRCGYC 1 cut(s) 182
Hin6I GCGC 3 cut(s) 1327, 1921, 3328
HinP1I GCGC 3 cut(s) 1327, 1921, 3328
HincII GTYRAC 4 cut(s) 888, 2449, 2641, 2999
HindII GTYRAC 4 cut(s) 888, 2449, 2641, 2999
HpaII CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
Hpy99I CGWCG 2 cut(s) 1096, 1863
HpyCH4III ACNGT 9 cut(s) 490, 568, 662, 775, 999, 1649, 2147, 2396, 3335
HpyCH4IV ACGT 7 cut(s) 710, 1094, 1951, 2451, 2509, 2635, 3205
HpyF10VI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 1711, 2251, 2441, 3350, 3420, 3495
HpySE526I ACGT 7 cut(s) 710, 1094, 1951, 2451, 2509, 2635, 3205
Hsp92I GRCGYC 1 cut(s) 182
HspAI GCGC 3 cut(s) 1327, 1921, 3328
KflI GGGWCCC 1 cut(s) 3479
Ksp22I TGATCA 2 cut(s) 2209, 2731
Kzo9I GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
LmnI GCTCC 7 cut(s) 5, 1384, 1442, 1702, 3325, 3350, 3559
Lsp1109I GCAGC 4 cut(s) 2003, 2998, 3286, 3443
MaeI CTAG 8 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894, 3005
MaeII ACGT 7 cut(s) 710, 1094, 1951, 2451, 2509, 2635, 3205
MalI GATC 8 cut(s) 144, 954, 1353, 1365, 2211, 2524, 2733, 3308
MbiI CCGCTC 2 cut(s) 2335, 3448
MboI GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
MfeI CAATTG 1 cut(s) 2436
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 6 cut(s) 1389, 1707, 2049, 2457, 2532, 3191
MlsI TGGCCA 1 cut(s) 2427
MluNI TGGCCA 1 cut(s) 2427
MlyI GAGTC 3 cut(s) 2915, 3386, 3494
MmeI TCCRAC 2 cut(s) 614, 1245
Mox20I TGGCCA 1 cut(s) 2427
Mph1103I ATGCAT 2 cut(s) 1402, 2877
MroXI GAANNNNTTC 2 cut(s) 156, 3165
MscI TGGCCA 1 cut(s) 2427
MseI TTAA 8 cut(s) 249, 375, 531, 1986, 2094, 2177, 2828, 2961
MslI CAYNNNNRTG 2 cut(s) 1130, 2211
Msp20I TGGCCA 1 cut(s) 2427
MspI CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
MunI CAATTG 1 cut(s) 2436
Mva1269I GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
MvaI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
MvnI CGCG 3 cut(s) 1863, 3056, 3330
MwoI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 1711, 2251, 2441, 3350, 3420, 3495
NciI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
NcoI CCATGG 2 cut(s) 1828, 3346
NdeI CATATG 1 cut(s) 3535
NdeII GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
NlaIV GGNNCC 6 cut(s) 606, 1704, 3189, 3480, 3481, 3541
NmuCI GTSAC 8 cut(s) 9, 218, 367, 521, 656, 1799, 3389, 3454
NsbI TGCGCA 1 cut(s) 1922
NsiI ATGCAT 2 cut(s) 1402, 2877
NspI RCATGY 8 cut(s) 440, 1070, 1135, 1319, 2161, 2875, 2972, 3220
NspV TTCGAA 1 cut(s) 833
PaeI GCATGC 1 cut(s) 2875
PagI TCATGA 3 cut(s) 139, 1839, 2206
PaqCI CACCTGC 1 cut(s) 1265
PciI ACATGT 3 cut(s) 1066, 2157, 3216
PctI GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
PdmI GAANNNNTTC 2 cut(s) 156, 3165
PfeI GAWTC 9 cut(s) 125, 275, 1823, 1945, 2357, 2477, 2661, 2720, 3211
PfoI TCCNGGA 1 cut(s) 824
PkrI GCNGC 7 cut(s) 801, 1993, 2334, 3013, 3276, 3433, 3467
PleI GAGTC 3 cut(s) 2914, 3386, 3494
PpsI GAGTC 3 cut(s) 2914, 3386, 3494
PpuMI RGGWCCY 1 cut(s) 3479
PscI ACATGT 3 cut(s) 1066, 2157, 3216
PshBI ATTAAT 1 cut(s) 2961
PsiI TTATAA 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 2049
Psp5II RGGWCCY 1 cut(s) 3479
Psp6I CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
PspEI GGTNACC 1 cut(s) 367
PspGI CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
PspN4I GGNNCC 6 cut(s) 606, 1704, 3189, 3480, 3481, 3541
PspOMI GGGCCC 1 cut(s) 3187
PspPI GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
PspPPI RGGWCCY 1 cut(s) 3479
PsrI GAACNNNNNNTAC 2 cut(s) 443, 475
PstI CTGCAG 3 cut(s) 1347, 1521, 2688
PstNI CAGNNNCTG 1 cut(s) 3603
PsuI RGATCY 1 cut(s) 1351
RsaI GTAC 8 cut(s) 574, 1690, 2080, 2138, 2231, 2344, 2417, 2728
RsaNI GTAC 8 cut(s) 573, 1689, 2079, 2137, 2230, 2343, 2416, 2727
RseI CAYNNNNRTG 2 cut(s) 1130, 2211
SacI GAGCTC 1 cut(s) 2049
SaqAI TTAA 8 cut(s) 249, 375, 531, 1986, 2094, 2177, 2828, 2961
SatI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
Sau3AI GATC 8 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306
Sau96I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
ScaI AGTACT 2 cut(s) 574, 2417
SchI GAGTC 3 cut(s) 2915, 3386, 3494
SduI GDGCHC 6 cut(s) 1389, 1707, 2049, 2457, 2532, 3191
SfcI CTRYAG 4 cut(s) 1343, 1404, 1517, 2684
SfuI TTCGAA 1 cut(s) 833
SinI GGWCC 2 cut(s) 490, 3479
SmiMI CAYNNNNRTG 2 cut(s) 1130, 2211
SmlI CTYRAG 5 cut(s) 161, 720, 2866, 2910, 3584
SmoI CTYRAG 5 cut(s) 161, 720, 2866, 2910, 3584
SphI GCATGC 1 cut(s) 2875
SsiI CCGC 8 cut(s) 799, 1375, 1592, 1863, 2333, 3303, 3448, 3466
SspI AATATT 1 cut(s) 484
SspMI CTAG 8 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894, 3005
SstI GAGCTC 1 cut(s) 2049
StyI CCWWGG 6 cut(s) 922, 1828, 1909, 2362, 2893, 3346
TaaI ACNGT 9 cut(s) 490, 568, 662, 775, 999, 1649, 2147, 2396, 3335
TaiI ACGT 7 cut(s) 713, 1097, 1954, 2454, 2512, 2638, 3208
TaqI TCGA 5 cut(s) 128, 648, 833, 951, 3151
TatI WGTACW 4 cut(s) 572, 1688, 2136, 2415
TauI GCSGC 3 cut(s) 802, 2335, 3468
TfiI GAWTC 9 cut(s) 125, 275, 1823, 1945, 2357, 2477, 2661, 2720, 3211
Tru1I TTAA 8 cut(s) 249, 375, 531, 1986, 2094, 2177, 2828, 2961
Tru9I TTAA 8 cut(s) 249, 375, 531, 1986, 2094, 2177, 2828, 2961
TseFI GTSAC 8 cut(s) 9, 218, 367, 521, 656, 1799, 3389, 3454
TseI GCWGC 4 cut(s) 1991, 3011, 3274, 3431
Tsp45I GTSAC 8 cut(s) 9, 218, 367, 521, 656, 1799, 3389, 3454
TspGWI ACGGA 2 cut(s) 39, 1336
VneI GTGCAC 1 cut(s) 2453
VpaK11BI GGWCC 2 cut(s) 490, 3479
VspI ATTAAT 1 cut(s) 2961
XapI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
XbaI TCTAGA 2 cut(s) 1285, 1393
XceI RCATGY 8 cut(s) 440, 1070, 1135, 1319, 2161, 2875, 2972, 3220
XcmI CCANNNNNNNNNTGG 1 cut(s) 2627
XmaJI CCTAGG 1 cut(s) 2893
XmiI GTMKAC 2 cut(s) 876, 3141
XmnI GAANNNNTTC 2 cut(s) 156, 3165
XspI CTAG 8 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894, 3005
ZrmI AGTACT 2 cut(s) 574, 2417
Zsp2I ATGCAT 2 cut(s) 1402, 2877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.