pycom15g21030

Thiosulfate sulfurtransferase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005733
Physical Location & Seq
Forward (+)
15059486 .. 15061731
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21030.1

Sequence Viewer

Length: 549 bp
ATGATGGCGGTTTCGTCTGTGTCATCCTCCTCTTGCTTTACTAGCTCTGCTGCAATTCAACATCCTTCACTTCTTTCTCACAAAAATCTCATCCACAAATCTTCGTTTTCTCTAAACGGGAAAAGACTTGGCACTGGACTCCAAAACAACAACTTCAACTTCTGTGTGAAAGCTTATCAGAAAAGGAATTTGGAGGCTGTTGGAGTTCCAACTTCCGTGCCAGTTCGTGTGGCTCACGAGCTTCTTCAAGCTGGACACAAATATTTGGATGTCAGGTCTCCTGAGGAGTTTAGTGCAGGGCATGCACCTGGGGCCGTTAACATCCCTTACTTGTACAAAGTTGGCTCAGGGATATCAAGGAACCCAGAATTTCTAAAAGAAGTGTCATCACATTTCAGAAAACATGATGAGATTATAGTTGGATGCCAGCTTGGGAAAAGGTCGATGATGGCTGCAACTGATCTTGTGGCTGCTGGTTTCACTGGCATTACAGATATAGCTGGTGGGTATGCTGTTTGGACGCAGACTGGACTTCCAACAGAGATATGA

Protein Analysis

183

Amino Acids

19.56

Weight (kDa)

9.22

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 79 - 175 8.4e-16 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 8
AcsI RAATTY 2 cut(s) 187, 368
AfaI GTAC 1 cut(s) 335
AgsI TTSAA 3 cut(s) 59, 157, 248
AjnI CCWGG 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 6 cut(s) 45, 173, 241, 251, 430, 500
AluI AGCT 6 cut(s) 45, 173, 241, 251, 430, 500
Alw26I GTCTC 1 cut(s) 282
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 3 cut(s) 50, 452, 470
ApoI RAATTY 2 cut(s) 187, 368
AspS9I GGNCC 1 cut(s) 312
AxyI CCTNAGG 1 cut(s) 282
BauI CACGAG 1 cut(s) 236
BbvI GCAGC 3 cut(s) 37, 439, 457
BccI CCATC 1 cut(s) 442
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 1 cut(s) 42
BisI GCNGC 3 cut(s) 51, 453, 471
BlsI GCNGC 3 cut(s) 52, 454, 472
Bme1390I CCNGG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 2 cut(s) 313, 362
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 413
Bpu10I CCTNAGC 1 cut(s) 346
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 308
Bse1I ACTGG 4 cut(s) 139, 221, 487, 532
Bse21I CCTNAGG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 6 cut(s) 23, 61, 90, 274, 321, 428
BseMII CTCAG 2 cut(s) 273, 360
BseNI ACTGG 4 cut(s) 139, 221, 487, 532
BseRI GAGGAG 2 cut(s) 19, 299
BseXI GCAGC 3 cut(s) 37, 439, 457
BsgI GTGCAG 1 cut(s) 315
BshFI GGCC 1 cut(s) 314
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 2 cut(s) 274, 359
BspLI GGNNCC 2 cut(s) 313, 362
BspTNI GGTCTC 1 cut(s) 282
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 4 cut(s) 139, 221, 487, 532
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 1 cut(s) 460
BssSI CACGAG 1 cut(s) 236
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 309
BstAPI GCANNNNNTGC 1 cut(s) 302
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 303, 428
BstDEI CTNAG 2 cut(s) 282, 346
BstF5I GGATG 6 cut(s) 23, 61, 90, 274, 321, 428
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 3 cut(s) 42, 302, 311
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 307
BstV1I GCAGC 3 cut(s) 37, 439, 457
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 1 cut(s) 314
BtsCI GGATG 6 cut(s) 23, 61, 90, 274, 321, 428
BtsIMutI CAGTG 2 cut(s) 132, 480
Cac8I GCNNGC 2 cut(s) 303, 428
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 1 cut(s) 529
Csp6I GTAC 1 cut(s) 334
CviAII CATG 2 cut(s) 302, 404
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 282, 346
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 354
Eco81I CCTNAGG 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 307
EcoRV GATATC 1 cut(s) 354
FaeI CATG 2 cut(s) 305, 407
FaiI YATR 6 cut(s) 303, 405, 416, 497, 510, 547
FatI CATG 2 cut(s) 301, 403
Fnu4HI GCNGC 3 cut(s) 51, 453, 471
FokI GGATG 6 cut(s) 10, 48, 77, 281, 308, 435
Fsp4HI GCNGC 3 cut(s) 51, 453, 471
FspBI CTAG 1 cut(s) 42
GluI GCNGC 3 cut(s) 51, 453, 471
HaeIII GGCC 1 cut(s) 314
HgaI GACGC 1 cut(s) 529
Hin1II CATG 2 cut(s) 305, 407
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 1 cut(s) 138
HpaI GTTAAC 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 2 cut(s) 180, 398
Hpy188III TCNNGA 2 cut(s) 236, 281
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 75
HpyCH4V TGCA 4 cut(s) 53, 296, 305, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 302, 311
HpyF3I CTNAG 2 cut(s) 282, 346
Hsp92II CATG 2 cut(s) 305, 407
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 1 cut(s) 460
Lsp1109I GCAGC 3 cut(s) 37, 439, 457
LweI GCATC 1 cut(s) 413
MaeI CTAG 1 cut(s) 42
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 2 cut(s) 93, 236
MluCI AATT 3 cut(s) 54, 187, 368
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 3 cut(s) 181, 233, 400
MnlI CCTC 4 cut(s) 37, 40, 187, 277
MseI TTAA 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 42, 302, 311
NdeII GATC 1 cut(s) 460
NlaIII CATG 2 cut(s) 305, 407
NlaIV GGNNCC 2 cut(s) 313, 362
NspI RCATGY 1 cut(s) 305
PaeI GCATGC 1 cut(s) 305
PkrI GCNGC 3 cut(s) 52, 454, 472
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 313, 362
PspPI GGNCC 1 cut(s) 312
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 3 cut(s) 51, 453, 471
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 309
SetI ASST 9 cut(s) 47, 175, 243, 253, 278, 310, 432, 443, 502
SfaNI GCATC 1 cut(s) 413
SphI GCATGC 1 cut(s) 305
Sse9I AATT 3 cut(s) 54, 187, 368
SsiI CCGC 1 cut(s) 8
SspI AATATT 1 cut(s) 263
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 307
TaqI TCGA 1 cut(s) 443
TasI AATT 3 cut(s) 54, 187, 368
TatI WGTACW 1 cut(s) 333
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 2 cut(s) 139, 487
TseI GCWGC 3 cut(s) 50, 452, 470
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 2 cut(s) 139, 487
XapI RAATTY 2 cut(s) 187, 368
XceI RCATGY 1 cut(s) 305
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.