Rw2G009250

Thiosulfate sulfurtransferase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005733
Physical Location & Seq
Forward (+)
9316450 .. 9318338
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009250.1

Sequence Viewer

Length: 543 bp
ATGTCTTCTTTGTCCACGTCTTGCTTCACTGGTTCTGTTCTTCGAATCCCTTCTCTTCTTTCTCCTCAGAATCTCAATCAGAGGTCTTTGTTGTCACTAACTGGGAACCCAGATCGAATTCGTGGCAATCGGATTGCATGCAAGAACTTAAACTTCTGTTCGAAGGCTAGTCTGAGAGGGAATTTGGAGGCCACCGGAGTTCCGACGTCGGTGCCGGTGCGTGTGGCTCACGAGCTTCTTCTAGCCGGACACAAGTATTTGGATGTCAGGACTCCTGAAGAGTTTAGTGCAGGACATCCTTCTGGTGCAGTTAACATTCCTTACTTGTACAAAGTTGGATCAGGGATGTCAAAGAACCCAGAGTTTGTGAAGGAAGTGTCATCACATTTCAGAAAACATGATGAAATTATTGTTGGATGTCAGCTCGGGAAAAGATCAATGATGGCTGCAACTGATCTTGTAGCTGCTGGTTTCTCTGGGATTACAGACATAGCTGGTGGGTATGCTGCTTGGACTCAAAATAGACTTCCAACAGAGTTGTGA

Protein Analysis

180

Amino Acids

19.36

Weight (kDa)

9.35

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 85 - 173 4e-15 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 211
AclWI GGATC 1 cut(s) 346
AcsI RAATTY 2 cut(s) 117, 181
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 1 cut(s) 329
AjiI CACGTC 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 4 cut(s) 235, 424, 464, 494
AluI AGCT 4 cut(s) 235, 424, 464, 494
AlwI GGATC 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 3 cut(s) 446, 464, 506
ApoI RAATTY 2 cut(s) 117, 181
Asp700I GAANNNNTTC 1 cut(s) 49
AsuII TTCGAA 2 cut(s) 43, 161
AvaI CYCGRG 1 cut(s) 425
BanI GGYRCC 1 cut(s) 211
BauI CACGAG 1 cut(s) 230
BbvI GCAGC 3 cut(s) 433, 451, 493
BccI CCATC 1 cut(s) 436
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BfaI CTAG 2 cut(s) 168, 242
BisI GCNGC 3 cut(s) 447, 465, 507
BlsI GCNGC 3 cut(s) 448, 466, 508
BmeT110I CYCGRG 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 107, 213
BmrI ACTGGG 1 cut(s) 111
BmuI ACTGGG 1 cut(s) 111
Bpu14I TTCGAA 2 cut(s) 43, 161
BsaHI GRCGYC 1 cut(s) 206
BsaWI WCCGGW 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 34, 106
BseGI GGATG 4 cut(s) 268, 295, 351, 422
BseMII CTCAG 2 cut(s) 80, 164
BseNI ACTGG 2 cut(s) 34, 106
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 3 cut(s) 433, 451, 493
BsgI GTGCAG 2 cut(s) 309, 327
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 3 cut(s) 195, 215, 246
BsnI GGCC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 2 cut(s) 43, 161
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 112, 338, 434, 454
BspANI GGCC 1 cut(s) 191
BspCNI CTCAG 2 cut(s) 79, 165
BspLI GGNNCC 2 cut(s) 107, 213
BspPI GGATC 1 cut(s) 346
BspT104I TTCGAA 2 cut(s) 43, 161
BspT107I GGYRCC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 2 cut(s) 34, 106
BssAI RCCGGY 1 cut(s) 214
BssMI GATC 4 cut(s) 112, 338, 434, 454
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
Bst6I CTCTTC 2 cut(s) 60, 273
BstACI GRCGYC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 327
BstBI TTCGAA 2 cut(s) 43, 161
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 2 cut(s) 66, 173
BstF5I GGATG 4 cut(s) 268, 295, 351, 422
BstKTI GATC 4 cut(s) 115, 341, 437, 457
BstMBI GATC 4 cut(s) 112, 338, 434, 454
BstNSI RCATGY 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 433, 451, 493
BsuRI GGCC 1 cut(s) 191
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 4 cut(s) 268, 295, 351, 422
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 214
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 138, 398
CviJI RGCY 9 cut(s) 167, 191, 227, 235, 245, 424, 446, 464, 494
CviKI_1 RGCY 9 cut(s) 167, 191, 227, 235, 245, 424, 446, 464, 494
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 66, 173
DpnI GATC 4 cut(s) 114, 340, 436, 456
DpnII GATC 4 cut(s) 112, 338, 434, 454
Eam1104I CTCTTC 2 cut(s) 60, 273
EarI CTCTTC 2 cut(s) 60, 273
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 117
FaeI CATG 2 cut(s) 141, 401
FaiI YATR 4 cut(s) 139, 399, 491, 504
FatI CATG 2 cut(s) 137, 397
Fnu4HI GCNGC 3 cut(s) 447, 465, 507
FokI GGATG 4 cut(s) 275, 282, 358, 429
Fsp4HI GCNGC 3 cut(s) 447, 465, 507
FspBI CTAG 2 cut(s) 168, 242
GluI GCNGC 3 cut(s) 447, 465, 507
HaeIII GGCC 1 cut(s) 191
HapII CCGG 3 cut(s) 195, 215, 246
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 2 cut(s) 141, 401
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 4 cut(s) 45, 70, 271, 514
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 3 cut(s) 195, 215, 246
Hpy166II GTNNAC 2 cut(s) 15, 313
Hpy188I TCNGA 6 cut(s) 69, 81, 132, 174, 204, 392
Hpy188III TCNNGA 4 cut(s) 230, 268, 275, 427
Hpy8I GTNNAC 2 cut(s) 15, 313
Hpy99I CGWCG 2 cut(s) 208, 211
HpyAV CCTTC 4 cut(s) 60, 157, 309, 364
HpyCH4IV ACGT 2 cut(s) 17, 206
HpyCH4V TGCA 5 cut(s) 137, 141, 290, 308, 449
HpyF3I CTNAG 2 cut(s) 66, 173
HpySE526I ACGT 2 cut(s) 17, 206
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 2 cut(s) 141, 401
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 112, 338, 434, 454
Lsp1109I GCAGC 3 cut(s) 433, 451, 493
MaeI CTAG 2 cut(s) 168, 242
MaeII ACGT 2 cut(s) 17, 206
MaeIII GTNAC 1 cut(s) 93
MalI GATC 4 cut(s) 114, 340, 436, 456
MboI GATC 4 cut(s) 112, 338, 434, 454
MboII GAAGA 4 cut(s) 32, 47, 230, 290
MluCI AATT 3 cut(s) 117, 181, 405
MlyI GAGTC 2 cut(s) 265, 508
MmeI TCCRAC 3 cut(s) 227, 316, 394
MnlI CCTC 4 cut(s) 75, 75, 170, 181
MroXI GAANNNNTTC 1 cut(s) 49
MseI TTAA 2 cut(s) 149, 312
MspI CCGG 3 cut(s) 195, 215, 246
NdeII GATC 4 cut(s) 112, 338, 434, 454
NlaIII CATG 2 cut(s) 141, 401
NlaIV GGNNCC 2 cut(s) 107, 213
NmuCI GTSAC 1 cut(s) 93
NspI RCATGY 1 cut(s) 141
NspV TTCGAA 2 cut(s) 43, 161
PaeI GCATGC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 2 cut(s) 45, 70
PkrI GCNGC 3 cut(s) 448, 466, 508
PleI GAGTC 2 cut(s) 265, 508
PpsI GAGTC 2 cut(s) 265, 508
PspN4I GGNNCC 2 cut(s) 107, 213
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 2 cut(s) 149, 312
SatI GCNGC 3 cut(s) 447, 465, 507
Sau3AI GATC 4 cut(s) 112, 338, 434, 454
SchI GAGTC 2 cut(s) 265, 508
SetI ASST 7 cut(s) 20, 86, 209, 237, 426, 466, 496
SfuI TTCGAA 2 cut(s) 43, 161
SphI GCATGC 1 cut(s) 141
Sse9I AATT 3 cut(s) 117, 181, 405
SspMI CTAG 2 cut(s) 168, 242
TaiI ACGT 2 cut(s) 20, 209
TaqI TCGA 3 cut(s) 43, 115, 161
TasI AATT 3 cut(s) 117, 181, 405
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 2 cut(s) 45, 70
Tru1I TTAA 2 cut(s) 149, 312
Tru9I TTAA 2 cut(s) 149, 312
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 1 cut(s) 93
TseI GCWGC 3 cut(s) 446, 464, 506
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 1 cut(s) 417
TspRI CASTG 1 cut(s) 34
XapI RAATTY 2 cut(s) 117, 181
XceI RCATGY 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 2 cut(s) 168, 242
ZraI GACGTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.