AT5G66040

Rhodanese-like domain-containing protein 15, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005733
Physical Location & Seq
Forward (+)
26410344 .. 26411355
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66040.1

Sequence Viewer

Length: 363 bp
ATGGCGGAGGAGAGCAGAGTCCCATCATCGGTGTCGGTAACAGTCGCCCACGACCTTCTTCTAGCCGGCCACCGCTATCTAGACGTCAGGACTCCAGAAGAGTTCAGCCAGGGACATGCCTGTGGGGCGATCAACGTGCCTTACATGAATCGAGGAGCATCAGGGATGTCAAAGAACCCCGACTTCCTGGAACAAGTCTCCTCCCATTTCGGACAATCCGATAACATCATTGTTGGCTGCCAGAGCGGTGGTAGATCTATCAAAGCCACCACCGATCTTCTCCATGCAGGTTTCACGGGAGTCAAAGATATCGTCGGTGGCTATAGTGCCTGGGCCAAGAATGGCCTTCCTACAAAGGCTTGA

Protein Analysis

120

Amino Acids

12.68

Weight (kDa)

6.49

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 25 - 113 7.9e-16 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 246
AciI CCGC 3 cut(s) 5, 73, 246
AcoI YGGCCR 1 cut(s) 67
AcyI GRCGYC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 28
AjnI CCWGG 3 cut(s) 108, 186, 329
Alw26I GTCTC 1 cut(s) 202
AoxI GGCC 3 cut(s) 67, 333, 343
ApeKI GCWGC 1 cut(s) 237
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
AspS9I GGNCC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 3 cut(s) 110, 188, 331
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 2 cut(s) 62, 80
BfmI CTRYAG 1 cut(s) 322
BfuAI ACCTGC 1 cut(s) 278
BglI GCCNNNNNGGC 1 cut(s) 125
BglII AGATCT 1 cut(s) 254
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 3 cut(s) 110, 188, 331
BmgT120I GGNCC 1 cut(s) 333
BmrFI CCNGG 3 cut(s) 110, 188, 331
BmsI GCATC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 109, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 65
BseBI CCWGG 3 cut(s) 110, 188, 331
BseDI CCNNGG 2 cut(s) 109, 330
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 28
BseRI GAGGAG 3 cut(s) 23, 168, 190
BseXI GCAGC 1 cut(s) 224
BshFI GGCC 3 cut(s) 69, 335, 345
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 2 cut(s) 5, 126
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 5, 126
BsnI GGCC 3 cut(s) 69, 335, 345
Bsp143I GATC 3 cut(s) 129, 254, 274
BspACI CCGC 3 cut(s) 5, 73, 246
BspANI GGCC 3 cut(s) 69, 335, 345
BspMI ACCTGC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 109, 330
BssMI GATC 3 cut(s) 129, 254, 274
BssNI GRCGYC 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 110, 188, 331
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 67
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 3 cut(s) 132, 257, 277
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 3 cut(s) 129, 254, 274
BstMWI GCNNNNNNNGC 2 cut(s) 125, 243
BstNI CCWGG 3 cut(s) 110, 188, 331
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 3 cut(s) 108, 186, 329
BstSFI CTRYAG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 248
BstYI RGATCY 1 cut(s) 254
BsuRI GGCC 3 cut(s) 69, 335, 345
BtsCI GGATG 1 cut(s) 171
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 333
CviAII CATG 3 cut(s) 116, 145, 284
CviJI RGCY 9 cut(s) 65, 69, 108, 237, 266, 321, 335, 345, 359
CviKI_1 RGCY 9 cut(s) 65, 69, 108, 237, 266, 321, 335, 345, 359
DpnI GATC 3 cut(s) 131, 256, 276
DpnII GATC 3 cut(s) 129, 254, 274
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 20
Eco32I GATATC 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 108, 186, 329
EcoRV GATATC 1 cut(s) 310
FaeI CATG 3 cut(s) 119, 148, 287
FaiI YATR 4 cut(s) 117, 146, 285, 324
FaqI GGGAC 2 cut(s) 5, 126
FatI CATG 3 cut(s) 115, 144, 283
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 2 cut(s) 62, 80
GluI GCNGC 1 cut(s) 238
GsuI CTGGAG 1 cut(s) 78
HaeIII GGCC 3 cut(s) 69, 335, 345
HapII CCGG 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 119, 148, 287
HinfI GANTC 4 cut(s) 18, 91, 148, 300
HpaII CCGG 1 cut(s) 66
Hpy188I TCNGA 2 cut(s) 212, 220
Hpy188III TCNNGA 3 cut(s) 80, 88, 95
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 2 cut(s) 65, 356
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 84, 135
HpyCH4V TGCA 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 243
HpySE526I ACGT 2 cut(s) 84, 135
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 119, 148, 287
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 129, 254, 274
LmnI GCTCC 1 cut(s) 155
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 167
MaeI CTAG 2 cut(s) 62, 80
MaeII ACGT 2 cut(s) 84, 135
MaeIII GTNAC 1 cut(s) 37
MalI GATC 3 cut(s) 131, 256, 276
MbiI CCGCTC 1 cut(s) 246
MboI GATC 3 cut(s) 129, 254, 274
MboII GAAGA 3 cut(s) 50, 110, 269
MflI RGATCY 1 cut(s) 254
MlyI GAGTC 3 cut(s) 27, 85, 309
MnlI CCTC 2 cut(s) 146, 211
MroNI GCCGGC 1 cut(s) 65
MslI CAYNNNNRTG 1 cut(s) 120
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 3 cut(s) 110, 188, 331
MvaI CCWGG 3 cut(s) 110, 188, 331
MwoI GCNNNNNNNGC 2 cut(s) 125, 243
NaeI GCCGGC 1 cut(s) 67
NdeII GATC 3 cut(s) 129, 254, 274
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 3 cut(s) 119, 148, 287
NspI RCATGY 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 148
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 3 cut(s) 26, 85, 308
PpsI GAGTC 3 cut(s) 26, 85, 308
Psp6I CCWGG 3 cut(s) 108, 186, 329
PspGI CCWGG 3 cut(s) 108, 186, 329
PspPI GGNCC 1 cut(s) 333
PsuI RGATCY 1 cut(s) 254
RseI CAYNNNNRTG 1 cut(s) 120
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 129, 254, 274
Sau96I GGNCC 1 cut(s) 333
SchI GAGTC 3 cut(s) 27, 85, 309
ScrFI CCNGG 3 cut(s) 110, 188, 331
SetI ASST 4 cut(s) 57, 87, 138, 292
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 120
SsiI CCGC 3 cut(s) 5, 73, 246
SspMI CTAG 2 cut(s) 62, 80
StyD4I CCNGG 3 cut(s) 108, 186, 329
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 2 cut(s) 87, 138
TaqI TCGA 1 cut(s) 151
TfiI GAWTC 1 cut(s) 148
TseI GCWGC 1 cut(s) 237
TspDTI ATGAA 1 cut(s) 161
XbaI TCTAGA 1 cut(s) 79
XceI RCATGY 1 cut(s) 119
XspI CTAG 2 cut(s) 62, 80
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.